Digital Commons Network^™

Osmosensitive Release Of Neurotransmitter Amino Acids: Relevance And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, Lenin Ochoa, Benito Ordaz

School of Veterinary and Biomedical Sciences: Faculty Publications

Hyposmolarity activates amino acid efflux as part of the corrective volume process in a variety of cells. This review discusses the mechanism of amino acid release in brain cells preparations. Results present evidence of substantial differences between the efflux of taurine and that of GABA and glu-tamate, which besides a possible role as osmolytes, have a main function as synaptic transmitters. The differences found concern the efflux time course, the sensitivity to Cl^– channel blockers, the modulation by tyrosine kinases, the influence of PKC and the effect of cytoskeleton disruptive agents. While taurine efflux features fit well with the …

Displacement Of Escherichia Coli O157:H7 From Rumen Medium Containing Prebiotic Sugars, Albane De Vaux, Mark Morrison, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

A fed-batch, anaerobic culture system was developed to assess the behavior of Escherichia coli O157:H7 in a rumen-like environment. Fermentation medium consisted of either 50% (vol/vol) raw or sterile rumen fluid and 50% phosphate buffer. Additional rumen fluid was added twice per day, and samples were removed three times per day to simulate the exiting of digesta and microbes from the rumen environment under typical feeding regimens. With both types of medium, anaerobic and enteric bacteria reached 10¹⁰ and 10⁴ cells/ml, respectively, and were maintained at these levels for at least 5 days. When a rifampin-resistant strain of …

Adenylyl Cyclase Functions Downstream Of The Gα Protein Gpa1 And Controls Mating And Pathogenicity Of Cryptococcus Neoformans, J. Andrew Alspaugh, Read Pukkila-Worley, Toshiaki Harashima, Lora M. Cavallo, Deanna L. Funnell, Gary M. Cox, John R. Perfect, James W. Kronstad, Joseph Heitman

Department of Plant Pathology: Faculty Publications

The signaling molecule cyclic AMP (cAMP) is a ubiquitous second messenger that enables cells to detect and respond to extracellular signals. cAMP is generated by the enzyme adenylyl cyclase, which is activated or inhibited by the Gα subunits of heterotrimeric G proteins in response to ligand-activated G-protein-coupled receptors. Here we identified the unique gene (CAC1) encoding adenylyl cyclase in the opportunistic fungal pathogen Cryptococcus neoformans. The CAC1 gene was disrupted by transformation and homologous recombination. In stark contrast to the situation for Saccharomyces cerevisiae, in which adenylyl cyclase is essential, C. neoformans cac1 mutant strains were viable and …

Suppressors Of Transcriptional Transgenic Silencing In Chlamydomonas Are Sensitive To Dna-Damaging Agents And Reactivate Transposable Elements, Byeong-Ryool Jeong, Dancia Wu-Scharf, Chaomei Zhang, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty Publications

In the unicellular green alga Chlamydomonas reinhardtii, the epigenetic silencing of transgenes occurs, as in land plants, at both the transcriptional and posttranscriptional levels. In the case of single-copy transgenes, transcriptional silencing takes place without detectable cytosine methylation of the introduced DNA. We have isolated two mutant strains, Mut-9 and Mut-11, that reactivate expression of a transcriptionally silenced single-copy transgene. These suppressors are deficient in the repression of a DNA transposon and a retrotransposon-like element. In addition, the mutants show enhanced sensitivity to DNA-damaging agents, particularly radiomimetic chemicals inducing DNA double- strand breaks. All of these phenotypes are much …

White-Light-Induced Fragmentation Of Toluene, A. M. Müller, B. Witzel, Cornelis J. Uiterwaal, J. Wanner, K.-L. Kompa

C.J.G.J. Uiterwaal Publications

We investigate the influence of continuum generation on the photofragmentation dynamics of the toluene molecule. By sending an 80-fs Ti:sapphire laser beam through a 2-mm-thick CaF₂ plate we show that even moderately broadened laser frequency spectra considerably increase the degree of fragmentation, while it is nearly absent without continuum generation. The measured broadening of the frequency spectra and the known Raman-active vibrational modes of toluene afford an explanation in terms of a stimulated Raman scattering scheme, in which the molecule absorbs large amounts of vibrational energy.

Vadose Zone Processes And Chemical Transport, H. M. Gaber, Steven Comfort, Patrick J. Shea, Thomas A. Machacek

School of Natural Resources: Faculty Publications

Permeable zerovalent iron (Fe⁰) barriers have become an estab- lished technology for remediating contaminated ground water. This same technology may be applicable for treating pesticides amenable to dehalogenation as they move downward in the vadose zone. By conducting miscible displacement experiments in the laboratory with metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl) acetamide; a chloroacetanilide herbicide] under unsaturated flow, we provide proof-of-concept for such an approach. Transport experiments were conducted in repacked, unsaturated soil columns attached to vacuum chambers and run under constant matrix potential (-30 kPa) and Darcy flux (approximately 2 cm d^-1 ). Treatments included soil …

Novel Algorithms For Remote Estimation Of Vegetation Fraction, Anatoly A. Gitelson, Yoram J. Kaufman, Robert Stark, Don Rundquist

School of Natural Resources: Faculty Publications

Spectral properties of a wheat canopy with vegetation fraction (VF) from 0% to 100% in visible and near-infrared (NIR) ranges of the spectrum were studied in order to devise a technique for remote estimation of VF. When VF was < 60%, from emergence till middle of the elongation stage, four distinct, and quite independent, spectral bands of reflectance existed in the visible range of the spectrum: 400 to 500 nm, 530 to 600 nm, near 670 nm, and around 700 nm. When VF was between 60% and 100%, reflectance in the NIR leveled off or even decreases with an increase of VF. The decreased reflectance in the NIR, occurring at or near the midseason, can be a limiting factor in the use of that spectral region for VF estimation. It was found that for VF > 60%, the information content of reflectance spectra in visible range can be expressed by only two independent pairs of spectral bands: (1) the blue from 400 to 500 nm and the red near 670 nm; (2) the green around 550 nm and the red edge region near 700 nm. We propose using only the visible range of the spectrum to quantitatively …

Selected Reflection Imaging: A Useful Tool For Imaging Nanocomposite Magnetic Materials, Yi Liu, Y. Qiang, M. Yu, J. Ping Liu, David J. Sellmyer

David Sellmyer Publications

The great strength of transmission electron microscope (TEM) is its capability to access to both real space (imaging) and reciprocal space (diffraction). Selected reflection imaging (SRI) can relate the diffraction of nanostructured materials with the image, providing information otherwise not possible. The experimental set up of SRI is given by Liu and Sellmyer [I]. In this paper we present some new measurements on nanocomposite magnetic materials.

Wildland Fire In Ecosystems Effects Of Fire On Air, David V. Sandberg, Roger D. Ottmar, Janice L. Peterson, John Core

Joint Fire Science Program Synthesis Reports

This state-of-knowledge review about the effects of fire on air quality can assist land, fire, and air resource managers with fire and smoke planning, and their efforts to explain to others the science behind fire-related program policies and practices to improve air quality. Chapter topics include air quality regulations and fire; characterization of emissions from fire; the transport, dispersion, and modeling of fire emissions; atmospheric and plume chemistry; air quality impacts of fire; social consequences of air quality impacts; and recommendations for future research.

The Magnetism-Nanostructure Interface In Advanced Magnetic Materials, David J. Sellmyer

David Sellmyer Publications

The critical relationship between microstructure and nanostructure of materials and their magnetic properties has been appreciated for decades. Electromagnetic machinery, permanent magnets, and data recording and electronic devices all have seen steady and sometimes spectacular advances over this period. At the present time the most interesting research in magnetism and magnetic materials arises from new developments in structuring materials on the nanometer length scale. This talk will present recent advances and challenges in furthering this work, with particular attention paid to extremely high density magnetic recording films, exchange-coupled high-energy-product permanent-magnet materials, high-temperature permanent-magnet materials, and self-organized and patterned magnetic nanoarrays.

Teachers And Scholars As Designers: The Art And Practice Of Instructional Design, Charles M. Spuches

Professional and Organizational Development Network in Higher Education: Archives

Instructional design theory and practice can help us meet increasing challenges, employ new knowledge and resources, and create optimal learning environments.

Community Services Implementation Project

University of Nebraska Public Policy Center: Publications

In November 2000, the University of Nebraska Public Policy Center (PPC) and the University of Nebraska-Lincoln Center on Children, Families and the Law (CCFL) entered into a partnership with the Lincoln/Lancaster County Joint Budget Committee (JBC) and United Way of Lincoln/Lancaster County (UW). The purpose of this University-Community partnership has been to facilitate implementation of the Lincoln/Lancaster County Human Services Three-Year Comprehensive Plan (Plan). It has since become known as C-SIP (the Community Services Implementation Project). PPC and CCFL have been serving in a coordinating and technical assistance role for the overall process including: providing staff support and technical assistance …

Geometric Patterns And The Interpretation Of Meaning: Two Monuments In Iran, Carol Bier

Textile Research Works

The Alhambra has often served in the West as the paradigm for understanding geometric pattern in Islamic art. Constructed in Spain in the 13th century as a highly defended palace, it is a relatively late manifestation of an Islamic fascination with geometric pattern. Numerous earlier Islamic buildings, from Spain to India, exhibit extensive geometric patterning, which substantiate a mathematical interest in the spatial dimension and its manifold potential for meaning. This paper examines two monuments on the Iranian plateau, dating from the 11 th century of our era, in which more than one hundred exterior surface areas have received patterns …

Regulation Of Endometrial Granulocyte Macrophage-Colony Stimulating Factor (Gm-Csf) In The Ewe, W. J. Mcguire, K. Imakawa, K. Tamura, C. S. R. Meka, R. K. Christenson

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Granulocyte macrophage-colony stimulating factor (GM-CSF) increases ovine interferon-tau (oIFNτ) secretion by ovine conceptuses, but endometrial production of GM-CSF has not been characterized. Endometrial GM-CSF expression was evaluated in ovariectomized ewes implanted with estradiol-17β (E₂) and/or progesterone (P₄) for 14 days, in day 14 cyclic and day 14 pregnant ewes. Relative levels of endometrial GM-CSF mRNA were 3-fold higher in E₂- and E₂/P₄-treated ewes than that of control or P₄-treated ovariectomized ewes. Levels of endometrial GM-CSF mRNA for cyclic ewes were similar to E₂- and E_{2 …}

Combating Drought Through Preparedness, Donald A. Wilhite

National Drought Mitigation Center: Faculty Publications

Drought is a complex, slow-onset phenomenon that affects more people than any other natural haz-ard and results in serious economic, social, and environmental impacts. Although drought affects virtually all climatic regimes and has significant consequences in both developed and developing countries, its impacts are especially serious in developing countries where dryland agriculture pre-dominates. The impacts of drought are often an indicator of unsustainable land and water manage-ment practices, and drought assistance or relief provided by governments and donors encourages land managers and others to continue these practices. This often results in a greater dependence on government and a decline in …

G02-1445 Understanding Vaccines, Dicky D. Griffin, Steve Ensley, David R. Smith, Grant Dewell

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide explains the basics of vaccine value, the differences between types of vaccines used in animals, and discusses vaccine selection and vaccination program development.

Vaccines are an important part of disease prevention and control. Like insurance, vaccines come at a cost, including the price of the vaccine, labor to administer the vaccine, localized tissue damage from vaccine injections, and increased metabolic demand of the animal causing potential performance loss during the time the animal is developing a proper immune response. The increased metabolic demand can cause the animal to look depressed and therefore may be confused with illness. This …

Safe By A Mile, Charlie Metro, Tom Altherr

University of Nebraska Press: Sample Books and Chapters

Charlie Metro's career runs the gamut of the specialties found in baseball-player, coach, manager, scout, inventor. Metro has lived baseball at every level from the Great Depression to today's multimillion dollar contracts. One of a kind, Metro's life mirrors the astounding changes in the game as well as in the nation. Metro's tale is full of heart and a wealth of anecdotes, the result of a fascinating life and a true love of the game.

Charlie Metro was born Charles Moreskonich in Nanty-Glo, Pennsylvania, in 1919. He played in the major leagues from 1943 to 1945 with the Detroit Tigers …

2002-03 Unopa Annual Report

UNOPA: Annual Reports

INSIDE THIS REPORT President’s Report ..................................... 1 President-elect’s Report .......................... 3 Financial Statement .................................. 4 Secretaries Reports .................................. 5 Standing Committee Reports ................ 6 Special Committee Reports .................. 17 National Activities .................................. 20 University Support .................................. 23

Giving Baculoviruses A Better Edge, Arthur H. Mcintosh, Cynthia L. Goodman, James J. Grasela, Ben Hardin

Agricultural Research Magazine

Scientific advancements built on an understanding of what nature already provides are leading to environmentally friendly crop-pest control by either biological agents or specifically designed synthetic antiinsect compounds. A case in point: research on baculoviruses.

Baculoviruses are rod-shaped DNA viruses, many of which begin their life cycle reproducing inside cells. In the nuclei of caterpillar cells infected with baculoviruses, viral progeny multiply and are incorporated into protective polyhedronshaped protein structures called occlusion bodies. Infected caterpillars die and contaminate the leaf surfaces with the occlusion bodies. Then, healthy caterpillars ingest the occlusion bodies and release the virus when feeding on contaminated …

Victim, Perpetrator, Family, And Incident Characteristics Of Infant And Child Homicide In The United States Air Force, Don R. Lucas, Kelley C. Wezner, Joel S. Milner, Thomas R. Mccanne, I. Nell Harris, Carla Monroe-Posey, John P. Nelson

United States Air Force: Publications

Objective: The present study describes factors related to fatal abuse in three age groups in the United States Air Force (USAF).

Method: Records from 32 substantiated cases of fatal child abuse in the USAF were independently reviewed for 60 predefined factors.

Results: Males were over-represented in young child victims (between 1 year and 4 years of age) and child victims (between 4 years and 15 years of age) but not in infant victims (between 24 hours and 1 year of age). African-American infant victims and perpetrators were over-represented. Younger victims were more likely to have been previously …

Introduction To Endangered Peoples Of Africa And The Middle East : Struggles To Survive And Thrive, Robert K. Hitchcock, Alan J. Osborn

Department of Anthropology: Faculty Publications

Endangered Peoples of Africa and the Middle East: Struggles to Survive and Thrive is about human populations residing in Africa and the Middle East, a diverse region that is connected geographically, culturally, and historically. The African continent is vast and covers 11.7 million square miles, or an area slightly larger than the combined area of the United States and South America (Table 1). Today, the African continent is home to some 771 million people distributed within fifty-four separate countries. Of the world's continents, Africa is by far the most diverse culturally. In Sudan, for example, there are over 200 ethnic …

Biomineralization Of Poorly Crystalline Fe(Iii) Oxides By Dissimilatory Metal Reducing Bacteria (Dmrb), John M. Zachara, Ravi K. Kukkadapu, James K. Fredrickson, Yuri A. Gorby, Steven C. Smith

United States Department of Energy: Publications

Dissimilatory metal reducing bacteria (DMRB) catalyze the reduction of Fe(III) to Fe(II) in anoxic soils, sediments, and groundwater. Two-line ferrihydrite is a bioavailable Fe(III) oxide form that is exploited by DMRB as a terminal electron acceptor. A wide variety of biomineralization products result from the interaction of DMRB with 2-line ferrihydrite. Here we describe the state of knowledge on the biotransformation of synthetic 2-line ferrihydrite by laboratory cultures of DMRB using select published data and new experimental results. A facultative DMRB is emphasized (Shewanella putrefaciens) upon which most of this work has been performed. Key factors controlling the identity of …

Influence Of Mn Oxides On The Reduction Of Uranium(Vi) By The Metal-Reducing Bacterium Shewanella Putrefaciens, James K. Fredrickson, John M. Zachara, David Kennedy, Chongxuan Liu, Martine Duff, Douglas Hunter, Alice Dohnalkova

United States Department of Energy: Publications

The potential for Mn oxides to modify the biogeochemical behavior of U during reduction by the subsurface bacterium Shewanella putrefaciens strain CN32 was investigated using synthetic Mn(III/IV) oxides (pyrolusite [β-MnO₂], bixbyite [Mn₂O₃] and K⁺-birnessite [K₄Mn₁₄O₂₇ • 8H₂O]). In the absence of bacteria, pyrolusite and bixbyite oxidized biogenic uraninite (UO₂[s]) to soluble U(VI) species, with bixbyite being the most rapid oxidant. The Mn(III/IV) oxides lowered the bioreduction rate of U(VI) relative to rates in their absence or in the presence of gibbsite …

A Saccharomyces Cerevisiae Gene Required For Heterologous Fatty Acid Elongase Activity Encodes A Microsomal Β-Keto-Reductase, Frédéric Beaudoin, Ken Gable, Olga Sayanova, Teresa Dunn, Johnathan A. Napier

Uniformed Services University of the Health Sciences

A number of Saccharomyces cerevisiae membranebound oxidoreductases were examined for potential roles in microsomal fatty acid elongation, by assaying heterologous elongating activities in individual deletion mutants. One yeast gene, YBR159w, was identified as being required for activity of both the Caenorhabditis elegans elongase PEA₁ (F₅₆H_11.4) and the Arabidopsis thaliana elongase FAE₁. Ybr159p shows some limited homology to human steroid dehydrogenases and is a member of the short-chain alcohol dehydrogenase superfamily. Disruption of YBR159w is not lethal, in contrast to previous reports, although the mutants are slow growing and display high temperature sensitivity. Both …

The Saccharomyces Cerevisiae Ybr159w Gene Encodes The 3-Ketoreductase Of The Microsomal Fatty Acid Elongase, Gongshe Han, Ken Gable, Sepp D. Kohlwein, Frédéric Beaudoin, Johnathan A. Napier, Teresa M. Dunn

Uniformed Services University of the Health Sciences

The YBR159w gene encodes the major 3-ketoreductase activity of the elongase system of enzymes required for very long-chain fatty acid (VLCFA) synthesis. Mutants lacking the YBR159w gene display many of the phenotypes that have previously been described for mutants with defects in fatty acid elongation. These phenotypes include reduced VLCFA synthesis, accumulation of high levels of dihydrosphingosine and phytosphingosine, and accumulation of medium-chain ceramides. In vitro elongation assays confirm that the ybr159Δ mutant is deficient in the reduction of the 3-ketoacyl intermediates of fatty acid elongation. The ybr159Δ mutant also displays reduced dehydration of the 3-OH acyl intermediates of fatty …

Unopa Notes 2002

UNOPA: Newsletters

No abstract provided.

Review Of The Background And Application Of Triolein-Containing Semipermeable Membrane Devices In Aquatic Environmental Study, Yibing Lu, Zijian Wang, James Huckins

United States Geological Survey: Staff Publications

This paper briefly reviews research on passive in situ samplers for aquatic environments but focuses on the development and application of the triolein-containing semipermeable membrane device in aquatic environmental monitoring. Special attention is paid to the calibration of the devices, quality control issues, and its potential uses in environmental assessments of aquatic contaminants. Also, the suitability of the technique for incorporation with selected bioassays is examined.

Elemental Mapping Of Co-Pr Nanostructured Powders By Eels Image Filtering, Yi Liu, C. Nelson, H. Tang, David J. Sellmyer

David Sellmyer Publications

In current extremely high density recording media design, the signal to noise ratio SNR is related to the number of magnetic grains N in a recording bit by
SNR = 10 log_l0 N .................................... (1)
In earlier studies we have found that a metallurgical grain can act as a magnetic grain when grains are magnetically decoupled by a non-magnetic phase [1,2]. Alternatively, several metallurgical grains can be exchange-coupled together when they are small [3]. An ideal morphology is one in which the non-magnetic atoms are segregated at the grain boundaries forming the non-magnetic phase while keeping the grains closely …

Wood Thrush Movements And Habitat Use: Effects Of Forest Management For Red-Cockaded Woodpeckers, Jason Lang, Larkin A. Powell, David Krementz, Michael J. Conroy

School of Natural Resources: Faculty Publications

We monitored adult and juvenile breeding-season movements and habitat use of radio-tagged Wood Thrushes (Hylocichla mustelina) at the Piedmont National Wildlife Refuge, central Georgia, USA. We investigated the effects that management for Red-cockaded Woodpeckers (Picoides borealis), thinning and burning >30 year old loblolly pine (Pinus taeda) habitat, had on Wood Thrushes, a ground-foraging and midstory-nesting species. Adult Wood Thrush pairs regularly moved long distances between nesting attempts (range 1 to 17,388 m). The only experimental effect we found on adult movements was a decrease in weekly emigration rates (AP) from thinned and burned compartments …

Conference Program: Hold Fast The Dream!

Professional and Organizational Development Network in Higher Education: Conference Materials

Welcome, by G. Roger Sell (President), the Conference Planning Team (Karron G. Lewis, Nancy Simpson, Marilla Svinicki, and Dorothy Zinsmeister), Sally Kuhlenschmidt (Program Chair)

Core Committee, 2002-2003

POD Presidents and Conference Sites

Conference Overview

Acknowledgements

POD Honored Presentation Awards in Recognition of Robert J. Menges

Schedule