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Ec98-752 Farm*A*Syst Nebraska’S System For Assessing Water Contamination Risk Worksheet 9: Livestock Manure Storage, Robert Grisso, Delynn Hay, Paul J. Jasa, Richard K. Koelsch, Sharon Skipton, Wayne Woldt

University of Nebraska-Lincoln Extension: Historical Materials

Why should I be concerned? Accumulating manure in storage incurs certain risks to the environment and to human and animal health unless appropriate precautions are taken.Manure can contribute nutrients and disease-causing organisms to both surface water and groundwater.

Flea News 55 (January 1998), Robert E. Lewis

Flea News

Miscellanea

Flea species

Material request

Siphonaptera literature

Mammalian Reservoirs And The Changing Epidemiology Of Rabies In The United States, James E. Childs, John W. Krebs, Charles E. Rupprecht

Vertebrate Pest Conference Proceedings: 18th (1998)

The epidemiology of rabies in the United States has changed dramatically over the past few decades. Greater than 90% of all animal rabies cases reported annually to the Centers for Disease Control and Prevention now occur in wildlife, whereas before 1960 the majority were domestic animals. The principal rabies reservoirs today are wild carnivores and bats, infected with many different types of rabies virus variants. Annual reporting of human deaths have fallen from more than 100 at the turn of the century to one to six per year, despite major outbreaks of animal rabies in several distinct geographic areas. Most …

Veterinary And Biomedical Sciences: Annual Report 1998

School of Veterinary and Biomedical Sciences: Information and History

Personnel
Awards and Recognition
Committees
Teaching Program
Graduate Program
GPVEC
Research Program
Extension Program
NVDLS
Active Grants
Publications
Presentations
Selected Committees, Editorial and Other Appointments
Nebraska Agricultural Statistics

Age-Dependent Characteristics Of Protection V. Susceptibility To Plasmodium Falciparum, J. Kevin Baird

United States Public Health Resources

Naturally acquired immunity to Plasmodium falciparum may be linked to key features of the immune system that change during normal development and ageing. Evidence of this was seen in non-immune Javanese transmigrants taking up residence in hyperendemic Irian Jaya, Indonesia. After 1± 2 years of residence, the adult migrants had less frequent and less intense parasitaemias than their children. Splenomegaly and malaria-like symptoms were also less common in the adults. These age-dependent patterns of relative resistance to P. falciparum mirrored those in lifelong residents. The Javanese adults acquired protective immunity against chronic exposure to infection relatively quickly compared with their …

Age-Dependent Susceptibility To Severe Disease With Primary Exposure To Plasmodium Falciparum, J. Kevin Baird, Sofyan Masbar, Hasan Basri, Soekartono Tirtokusumo, Budi Subianto, Stephen L. Hoffman

United States Public Health Resources

This study investigated the incidence of severe disease following primary exposure to Plasmodium falciparum by nonimmune children and adults in Irian Jaya, Indonesia. Four months after arrival, the cross-sectional prevalence of P. falciparum was 72%, and the monthly cumulative incidence of clinical diagnoses of malaria was 81%. Delirium or unconsciousness prompted evacuation to the hospital. Records of emergency evacuation of persons with a clinical diagnosis of malaria revealed an incidence density among adults (>15 years) of 1.34 events/person-year in the third month, whereas the rate in children remained stable at ~0.25 events/person-year( relative risk = 4.51, 95% confidence interval …

A Prolonged Outbreak Of Shigella Sonnei Infections In Traditionally Observant Jewish Communities In North America Caused By A Molecularly Distinct Bacterial Subtype, Jeremy Sobel, Daniel N. Cameron, Johanne Ismail, Nancy Strockbine, Michael Williams, Pamela S. Diaz, Barbara Westley, Marilyn Rittmann, Joseph Dicristina, Halina Ragazzoni, Robert V. Tauxe, Eric D. Mintz

United States Public Health Resources

During 1994–1996, Shigella sonnei outbreaks occurred in 8 North American traditionally observant Jewish communities. These communities remain relatively separate from neighboring populations while maintaining close contact by travel with coreligionists in other cities. Epidemiologic investigations suggested community-to-community transmission via travel. Outbreak-related and control isolates of S. sonnei from each city were subtyped by pulsed-field gel electrophoresis (PFGE) to confirm an epidemiologic linkage between outbreaks. Forty-three (94%) of 46 outbreak-related isolates had closely related PFGE patterns, constituting a single subtype; 33 (94%) of 35 control isolates demonstrated unrelated PFGE patterns. Several patterns differing by ≤3 bands were identified within the …

Human Herpesvirus 8 As A Potential Sexually Transmitted Agent In Honduras, C. Sosa, W. Klaskala, B. Chandran, R. Soto, L. Sieczkowski, M. H. Wu, M. Baum, Charles Wood

Nebraska Center for Virology: Faculty Publications

The seroprevalence of human herpesvirus 8 (HHV-8) was studied in 326 human immunodeficiency virus (HIV)–positive and –negative persons from Honduras; women constituted 77% (n = 251) of the subjects. Sera were tested for lytic HHV-8 antibodies by an IFA, and positive samples were confirmed by a radioimmunoprecipitation assay. Of the 326 persons tested, 58 (17.8%) had HHV- 8 antibodies. Among the HIV-infected women, 22.7% were seropositive; 11.3% of the HIV-negative women were seropositive. HHV-8 seroprevalence was almost four times higher in HIV-positive female commercial sex workers (36%) than in HIV-negative female non–commercial sex workers (9.9%; odds ratio = 3.8, 95% …

Seroprevalence Of Human Herpesvirus 8 Among Zambian Women Of Childbearing Age Without Kaposi’S Sarcoma (Ks) And Mother-Child Pairs With Ks, Jun He, Ganapati Bhat, Chipepo Kankasa, Chifumbe Chintu, Charles Mitchell, Wenjing Duan, Charles Wood

Nebraska Center for Virology: Faculty Publications

The seroprevalence of human herpesvirus 8 (HHV-8) among a group of Zambian women of reproductive age and among mother-child pairs in which either one of them has Kaposi’s sarcoma (KS) was determined. A cross-sectional group of 378 pregnant women was randomly recruited into the study, and 183 (48.4%) had HHV-8 antibodies. Among the human immunodeficiency virus (HIV)-1–infected women, 51.1% were HHV-8–seropositive, whereas of HIV- 1–negative women, 47.3% were HHV-8–seropositive. In addition, 21 women index patients with KS and 5 young children index patients with KS were studied. All children with KS had mothers who were HHV-8–seropositive, while not all children …

Ec98-761 Farm*A*Syst Nebraska’S System For Assessing Water Contamination Worksheet 10: Livestock Yards Management, Robert Grisso, Delynn Hay, Paul J. Jasa, Richard K. Koelsch, Sharon Skipton, Wayne Woldt

University of Nebraska-Lincoln Extension: Historical Materials

Why should I be concerned? Livestock yards, such as barnyards, holding areas and feedlots, are areas of concentrated livestock wastes and a potential source of nutrient, solids, and bacteria contamination of surface and groundwater. Yards that are abandoned permanently or are inactive temporarily also represent a potential risk.

Genetic Variation In North American Black Flies In The Subgenus Psilopelmia (Simulium: Diptera: Simuliidae), Jianming Tang, John K. Moulton, Kenneth Pruess, Eddie W. Cupp, Thomas R. Unnasch

Department of Entomology: Faculty Publications

Resolution of the genetic heterogeneity of closely related insect species depends on the selection of reliable genetic markers derived from representative specimens. We report the results of a survey of genetic variability in nine species of black flies in the subgenus Psilopelmia Enderlein. Three regions of the mitochondrial genome and an amplicon including the internal transcribed spacer 1 of the nuclear ribosomal RNA gene cluster (ITS1) were amplified using the polymerase chain reaction (PCR), and the amplicons were examined for intraspecific and interspecific polymorphisms. Six of the seven Psilopelmia species that yielded PCR products in the ITS1 PCR reaction were …

A Mouse Model For Evaluation Of Prophylaxis And Therapy Of Ebola Hemorrhagic Fever, Mike Bray, Kelly Davis, Tom Geisbert, Connie Schmaljohn, John Higgins

United States Army: Publications

The Zaire subtype of Ebola virus (EBO-Z) is lethal for newborn mice, but adult mice are resistant to the virus, which prevents their use as an animal model of lethal Ebola infection. We serially passed EBO-Z virus in progressively older suckling mice, eventually obtaining a plaque-purified virus that was lethal for mature, immunocompetent BALB/c and C57BL/6 inbred and ICR (CD-1) outbred mice. Pathologic changes in the liver and spleen of infected mice resembled those in EBOZ– infected primates. Virus titers in these tissues reached 109 pfu/g. The LD50 of mouse-adapted EBO-Z virus inoculated into the peritoneal cavity was ~1 virion. …

Polyadenylation Of Vesicular Stomatitis Virus Mrna Dictates Efficient Transcription Termination At The Intercistronic Gene Junctions, Leroy N. Hwang, Nathan Englund, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The intercistronic gene junctions of vesicular stomatitis virus (VSV) contain conserved sequence elements that are important for polyadenylation and transcription termination of upstream transcript as well as reinitiation of transcription of downstream transcript. To examine the role of the putative polyadenylation signal 3' AUACU₇5' at the gene junctions in polyadenylation and transcription termination, we constructed plasmids encoding antigenomic minireplicons containing one or two transcription units. In plasmid-transfected cells, analyses of the bicistronic minireplicon containing the wild-type or mutant intercistronic gene junctions for the ability to direct synthesis of polyadenylated upstream, downstream, and readthrough mRNAs showed that the AUACU …

On The Proper Definition Of Cognitive Ethology, Alan Kamil

Papers in Behavior in Biological Sciences

The last 20-30 years have seen two “scientific revolutions” in the study of animal behavior: the cognitive revolution that originated in psychology, and the Darwinian, behavioral ecology revolution that originated in biology. Among psychologists, the cognitive revolution has had enormous impact. Similarly, among biologists, the Darwinian revolution has had enormous impact. The major theme of this chapter is that these two scientific research programs need to be combined into a single approach, simultaneously cognitive and Darwinian, and that this single approach is most appropriately called cognitive ethology.

Revised List Of Type Specimens On Deposit In The University Of California Davis Nematode Collection, S. Patricia Stock, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The list of deposited type specimens is updated for the University of California Davis Nematode Collection, as recommended by the International Code of Zoological Nomenclature. The type collection includes 1,001 species and more than 11,000 individual specimens mounted on microscope slides. This list can be used as a reference to locate specimens but is not meant to clarify ambiguities that may exist concerning the type status of particular specimens.

Metazoan Parasites And Other Symbionts Of Cetaceans In The Caribbean, Antonio A. Mignucci-Giannoni, Eric P. Hoberg, Doug Siegel-Causey, Ernest H. Williams Jr.

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The parasite fauna in cetaceans from Puerto Rico, the Virgin Islands, and the larger Caribbean region is poorly known. We provide the first records for parasite biodiversity among a diverse assemblage of cetaceans from the Caribbean Sea. Internal and external parasites and commensals were collected from stranded whales and dolphins salvaged in Puerto Rico, the United States and British Virgin Islands, Jamaica, and Venezuela between 1989 and 1997. A total of 47 individuals of 16 species of whales and dolphins (15 odontocetes and one mysticete) were examined. Overall, parasites and commensals were found in 34 (72.3%) animals, representing 13 species …

The Identification And Characteristics Of Echinoparyphium Rubrum (Cort, 1914) Comb. New (Trematoda, Echinostomatidae) Based On Experimental Evidence Of The Life Cycle, Ivan Kanev, Robert Sorensen, Mauritz Strerner, Rebecca Cole, Bernard Fried

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The life cycle of Echinoparyphium rubrum (Cort, 1914) comb. n. has been completed experimentally. All of the developmental stages - egg, miracidium, sporocyst, mother and daughter rediae, cercaria, metacercaria, and adult - were examined and described. The miracidia infected freshwater snails of the genus Physa, P. gyrina and P. occidentalis. Attempts to infect snails of the genera Lymnaea, L. auricularis, L. peregra. L. truncatula and Bulinus, B. truncatus failed. Cercariae infected various pulmonate and prosobranch freshwater snails, mussels, frogs, water turtles and planarians. The adults developed in the small intestine of birds and mammals. The identity and major characteristics of …

Mummies And Mummification Practices In The Southern And Southwestern United States, Mahmoud Y. El-Najjar, Thomas M. J. Mulinski, Karl Reinhard

Karl Reinhard Publications

Mummification was not intentional for most North American prehistoric cultures. Natural mummification occurred in the dry areas of North America, where mummies have been recovered from rock shelters, caves, and overhangs. In these places, corpses desiccated and spontaneously mummified. In North America, mummies are recovered from four main regions: the southern and southwestern United States, the Aleutian Islands, and the Ozark Mountains of Arkansas. This chapter is limited to a discussion of burial practices and a tabulation of the location of mummies in the southwestern United States with some comments on mummies of the southern United States, including the Ozarks.

New Investigative Techniques [In Mummies, Disease & Ancient Cultures], Theodore A. Reyman, Henrick Nielsen, Ingolf Thuesen, Derek N. H. Notman, Karl Reinhard, Edmund Tapp, Tony Waldron

Karl Reinhard Publications

In this chapter, we shall see how the amplification of minute amounts of genetic material by the polymerase chain reaction (PCR) has enabled researchers to identifY both native and infectious DNA in these bodies. The use of amplified bacterial and viral DNA may turn out to be Aidan Cockburn's 'magic bullet' for solving some of the mysteries of infectious disorders in ancient populations. In the same fashion, native DNA may give us clues to a variety of genetic abnormalities that existed in those distant times. These newer techniques will be the focus of the revised technical section in this second …

Human Dental Microwear Caused By Calcium Oxalate Phytoliths In Prehistoric Diet Of The Lower Pecos Region, Texas, Dennis Danielson, Karl Reinhard

Karl Reinhard Publications

Recent research demonstrates that silica phytoliths of dietary origin are associated with microwear of human teeth. Previous research has shown that severe enamel microwear and dental wear characterizes Archaic hunter-gatherers in the lower Pecos region of west Texas. Calcium oxalate crystals are especially common in Archaic coprolites. The vast majority are derived from prickly pear and agave, which were the dietary staples in west Texas for 6,000 years. The calcium oxalate phytoliths are harder than enamel. Therefore, calcium oxalate crystals are the most likely source of previously documented dental microwear and wear in the lower Pecos region.Am J Phys Anthropol …

Comparisons Of Two Polymorphic Species Of Ostertagia And Phylogenetic Relationships Within The Ostertagiinae (Nematoda: Trichostrongyloidea) Inferred From Ribosomal Dna Repeat And Mitochondrial Dna Sequences, Dante S. Zarlenga, Eric P. Hoberg, Frank Stringfellow, J. Ralph Lichtenfels

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The first internal transcribed spacer DNA (ITS-1) (rDNA) and the mitochondrial (mt) DNA-derived cytochrome oxidase I gene (COX-1) were enzymatically amplified, cloned and sequenced from 6 nominal species of Ostertagiinae as well as Haemonchus contortus and Haemonchus placei. The portion of the COX-1 gene analyzed was 393 base pairs (bp) in length and contained 33 within species polymorphic base changes at 28 synonymous sites. The ITS-1 rDNA consensus sequences ranged from 392 bp (Ostertagia ostertagi/Ostertagia lyrata, Teladorsagia circumcincta) to 404 bp (H. contortus, H. placei). These data were used both in …

Stretching The Limits Of The Ada: Asymptomatic Hiv-Positive Status As A Disability In Bragdon V. Abbott, 118 S. Ct. 2196 (1998), Teresa A. Schneider

Nebraska Law Review

This Note points out problems with the Bragdon v. Abbott Supreme Court decision and ultimately argues that an asymptomatic HIV-positive person is not disabled under the first definition of disability contained in the Americans with Disabilities Act (ADA); that is, “a physical or mental impairment that substantially limits one or more of the major life activities of such individual.” An asymptomatic HIV-positive individual is not disabled within that definition because, even though he or she suffers from a physical impairment, the impairment does not substantially limit any major life activity. Although this Note rejects the argument that reproduction is a …

Basic Immunology, Lynn F. Woodward

Range Beef Cow Symposium

The immune system can be thought of as a surveillance system to discriminate between “self" and "non-self." From simple life forms such as insects to advanced life forms such as humans, all living organisms have some form of host defense mechanism. Most have multiple, overlapping mechanisms ranging from very non-specific resistance to highly specific immunity. White blood cells forming "pus" around a splinter is representative of non-specific host resistance to invasion while immunity to IBR virus after vaccination or following recovery from active infection is representative of specific active immunity.

These host defense systems protect livestock and man from the …

Porcine Reproductive And Respiratory Syndrome Virus Replicates In Testicular Germ Cells, Alters Spermatogenesis, And Induces Germ Cell Death By Apoptosis, Jung-Hyang Sur, Alan R. Doster, James S. Christian, Judith A. Galeota, Robert W. Wills, Jeff J. Zimmerman, Fernando A. Osorio

Nebraska Center for Virology: Faculty Publications

Like other arteriviruses, porcine reproductive and respiratory syndrome virus (PRRSV) is shed in semen, a feature that is critical for the venereal transmission of this group of viruses. In spite of its epidemiological importance, little is known of the association of PRRSV or other arteriviruses with gonadal tissues. We experimentally infected a group of boars with PRRSV 12068-96, a virulent field strain. By combined use of in situ hybridization and immunohistochemistry, we detected infection by PRRSV in the testes of these boars. The PRRSV testicular replication in testis centers on two types of cells: (i) epithelial germ cells of the …

Genetic Polymorphism Among Cryptosporidium Parvum Isolates: Evidence Of Two Distinct Human Transmission Cycles, Michael M. Peng, Lihua Xiao, Amanda R. Freeman, Michael J. Arrowood, Ananias A. Escalante, André C. Weltman, Corinne S.L. Ong, William R. Mac Kenzie, Altaf A. Lal, Charles B. Beard

Harold W. Manter Laboratory of Parasitology: Library Materials

We report the results of molecular analysis of 39 isolates of Cryptosporidium parvum from human and bovine sources in nine human outbreaks and from bovine sources from a wide geographic distribution. All 39 isolates could be divided into either of two genotypes, on the basis of genetic polymorphism observed at the thrombospondin-related adhesion protein (TRAP-C2) locus. Genotype 1 was observed only in isolates from humans. Genotype 2, however, was seen in calf isolates and in isolates from a subset of human patients who reported direct exposure to infected cattle or consumed items thought to be contaminated with cattle feces. Furthermore, …

Evolution Of The Schistosomes (Digenea: Schistosomatoidea): The Origin Of Dioecy And Colonization Of The Venous System, Thomas R. Platt, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Trematodes of the family Schistosomatidae are considered venous system specialists whose sister group is the vascular system generalists (Spirorchidae) of turtles. Colonization of homeotherms by vascular trematodes required precision egg laying near the conduit for egg passage to the external environment and avoidance of pathogenesis that might result in the premature death of the host. Evolution of dioecy from the hermaphroditic condition may have proceeded through androdioecy in which hermaphrodites were specialized for precision egg placement in the vascular system and larger adults became functional males. The evolution of nuclear genes suppressing female function along with cytoplasmic genes suppressing male …

Limited Range Of Genetic Variation In Echinococcus Multilocularis, Heinz Rinder, Robert L. Rausch, Kenichi Takahashi, Hartmuth Kopp, Angelika Thomschke, Thomas Löscher

Harold W. Manter Laboratory of Parasitology: Faculty Publications

DNA sequencing of 1.3 kb of rDNA containing both internal transcribed spacers (ITS1, ITS2) and adjoining rRNA coding regions in each of 11 Echinococcus multilocularis isolates from Germany, Japan, and Alaska resulted in identical nucleotide sequences except for a single polymorphic locus 54 bp upstream of the 3' end of the 18S coding region, separating Eurasian isolates from an Alaskan isolate. The same base substitution was found in each of two additional isolates from Alaska. The distribution of the resulting genotypes with regard to their origin is highly significant (>99.9%) and corresponds to the traditional subspecies Echinococcus multilocularis multilocularis …

A Revision Of The Calicotylinae Monticelli, 1903 (Monogenea: Monocotylidae), Leslie A. Chisholm, Tom J. Hansknecht, Ian D. Whittington, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A total of 153 elasmobranchs (46 species), either freshly collected from the Gulf of Mexico, USA, and Tasmania, Australia, or museum specimens collected from various localities worldwide, were examined for calicotyline (Monocotylidae) monogeneans. Thirty-five elasmobranchs, representing 17 species, were infected with Calicotyle spp. which we identified as the following previously described species: C. asterii (Szidat, 1970) Timofeeva, 1985; C. kroyeri Diesing, 1850; C. macrocotyle Cordero, 1944; C. similis (Szidat, 1972) Timofeeva, 1985; C. splendens (Szidat, 1970) Timofeeva, 1985; C. stossichi Braun, 1899; and C. urolophi Chisholm, Beverley-Burton & Last, 1991. The Calicotylinae, which comprises the genera Calicotyle and Dictyocotyle, …

Commentary On Linda Mealey (1995), The Sociobiology Of Sociopathy: An Integrated Evolutionary Model, Scott F. Stoltenberg, Joseph L. Hernandez Cruz, Don Joseph Stein, Linda Mealey

Department of Psychology: Faculty Publications

[SFS] When Genotype × Environment (G × E) interactions are present, heritability estimates are not interpretable. Mealey cites abundant evidence for G × E interactions in the etiology of sociopathy, thereby completely undermining estimates of the heritability of sociopathy which form the foundation of her model. Without proper evidence for a genetic basis of sociopathy, Mealey’s sociobiological model collapses under its own great weight.

[LM] It is impossible to discuss the constructs “heritability,” “theory of mind,” and “normality” in a single coherent essay. The following three rejoinders address each of these exceedingly complex constructs individually, as each relates to the …

Evolutionary Constraints On Population Structure: The Parasites Of Fundulus Zebrinus (Pisces: Cyprinodontidae) In The South Platte River Of Nebraska, John J. Janovy Jr., Scott D. Snyder, Richard E. Clopton

John J. Janovy, Jr. Publications

Population and community descriptor values (parasites per host, prevalence per parasite species, variance/mean ratios, species density, and diversity indices) for the 7-species parasite community of 61 relatively homogeneous samples of Fundulus zebrinus (Pisces: Cyprinodontidae) in the South Platte River of Nebraska, U.S.A., taken over a 14-yr period, are reported. South Platte River streamflow fluctuates over two orders of magnitude on several time scales-monthly, annually, and over multiple year wet-dry cycles. Relatively homogeneous sampling of a single host species with several parasite species provided a system that allowed assessment of the contribution of evolved parasite life cycles to population structure in …