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The Nematode Fauna Of Long-Nosed Mice Oxymycterus Spp. From The Bolivian Yungas, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner Apr 2003

The Nematode Fauna Of Long-Nosed Mice Oxymycterus Spp. From The Bolivian Yungas, F. Agustin Jimenez-Ruiz, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

During a long-term survey of the parasites of mammals from all over Bolivia, 44 individuals of Oxymycterus inca and 6 of Oxymycterus paramensis were collected and examined for parasites from the foothills and Eastern Cordillera (Los Yungas) of the Andes of Bolivia. Three species of nematode were found including, from the cecum, a previously unknown genus and species of pinworm, Caroloxyuris boliviensis n. gen, n. sp., aspidoderids representing Nematomystes rodentophilus, and from the stomach, Protospirura numidica criceticola. These helminths occurred in prevalences of 25, 9, and 14%, respectively, in O. paramensis. Caroloxyuris boliviensis resembles species included in …

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Prevalence Of Antibodies To Neospora Caninum, Sarcocystis Neurona, And Toxoplasma Gondii In Wild Horses From Central Wyoming, J. P. Dubey, S.M. Mitchell, J.K. Morrow, J.C. Rhyan, L.M. Stewart, D.E. Granstrom, S. Romand, P. Thulliez, W.J. Saville, D.S. Lindsay Mar 2003

Prevalence Of Antibodies To Neospora Caninum, Sarcocystis Neurona, And Toxoplasma Gondii In Wild Horses From Central Wyoming, J. P. Dubey, S.M. Mitchell, J.K. Morrow, J.C. Rhyan, L.M. Stewart, D.E. Granstrom, S. Romand, P. Thulliez, W.J. Saville, D.S. Lindsay

United States Department of Agriculture Wildlife Services: Staff Publications

Sarcocystis neurona, Neospora caninum, N. hughesi, and Toxoplasma gondii are 4 related coccidians considered to be associated with encephalomyelitis in horses. The source of infection for N. hughesi is unknown, whereas opossums, dogs, and cats are the definitive hosts for S. neurona, N. caninum, and T. gondii, respectively. Seroprevalence of these coccidians in 276 wild horses from central Wyoming outside the known range of the opossum (Didelphis virginiana) was determined. Antibodies to T. gondii were found only in 1 of 276 horses tested with the modified agglutination test using 1: 25, 1:50, …

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Paleopharmacology And Pollen: Theory, Method, And Application, Sérgio A.M. Chaves, Karl J. Reinhard Feb 2003

Paleopharmacology And Pollen: Theory, Method, And Application, Sérgio A.M. Chaves, Karl J. Reinhard

School of Natural Resources: Faculty Publications

Parasitism was a universal human condition. Because of this, people developed herbal medicines to treat parasites as part of their pharmacopoeias. We propose that it is possible to recover evidence of medicinal plants from archaeological sites and link their use to specific health conditions. This is a multidisciplinary approach that must involve at least paleoethnobotanists, archaeoparasitologists, paleopathologists, and pharmacologists.

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Inca Expansion And Parasitism In The Lluta Valley: Preliminary Data, Santoro Calagero, Karl J. Reinhard, Sheila D. Vinton Feb 2003

Inca Expansion And Parasitism In The Lluta Valley: Preliminary Data, Santoro Calagero, Karl J. Reinhard, Sheila D. Vinton

School of Natural Resources: Faculty Publications

Assessing the impact of cultural change on parasitism has been a central goal in archaeoparasitology. The influence of civilization and the development of empires on parasitism has not been evaluated. Presented here is a preliminary analysis of the change in human parasitism associated with the Inca conquest of the Lluta Valley in Northern Chile. Changes in parasite prevalence are described. It can be seen that the change in life imposed on the inhabitants of the Lluta Valley by the Incas caused an increase in parasitism.

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Parasite Remains In Archaeological Sites, Françoise Bouchet, Niéde Guidon, Katharina Dittmar, Stephanie Harter, Luiz F. Ferreira, Sérgio A.M. Chaves, Karl J. Reinhard, Adauto Araújo Feb 2003

Parasite Remains In Archaeological Sites, Françoise Bouchet, Niéde Guidon, Katharina Dittmar, Stephanie Harter, Luiz F. Ferreira, Sérgio A.M. Chaves, Karl J. Reinhard, Adauto Araújo

School of Natural Resources: Faculty Publications

Organic remains can be found in many different environments. They are the most significant source for paleoparasitological studies as well as for other paleoecological reconstruction. Preserved paleoparasitological remains are found from the driest to the moistest conditions. They help us to understand past and present diseases and therefore contribute to understanding the evolution of present human sociality, biology, and behavior. In this paper, the scope of the surviving evidence will be briefly surveyed, and the great variety of ways it has been preserved in different environments will be discussed. This is done to develop to the most appropriated techniques to …

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A Comprehensive Review Of The Ecological And Human Social Effects Of Artificial Feeding And Baiting Of Wildlife, L. Dunkley, M.R.L. Cattet Feb 2003

A Comprehensive Review Of The Ecological And Human Social Effects Of Artificial Feeding And Baiting Of Wildlife, L. Dunkley, M.R.L. Cattet

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

In recent years, events within Canada and the United States have drawn attention to potential negative consequences of feeding and baiting wild animals, especially enhanced transmission of infectious diseases such as bovine tuberculosis and chronic wasting disease. This report was prepared to gather available science-based information on the ecological and human social effects of artificial feeding and baiting of wildlife into one readily accessible document. The contracting agencies, Parks Canada and Saskatchewan Environment, recognize that an objective review of existing literature may help to answer questions and concerns within and outside the agencies, and help to guide subsequent decision-making concerning …

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Host Specificity Among Ancyrocephalinae (Monogenoidea) Of Nebraska Sunfish, Megan R. Collins, John J. Janovy Jr. Feb 2003

Host Specificity Among Ancyrocephalinae (Monogenoidea) Of Nebraska Sunfish, Megan R. Collins, John J. Janovy Jr.

John J. Janovy, Jr. Publications

This study addressed the problem of local patterns of host specificity among Ancyrocephalinae (Monogenoidea) on bass and sunfish species, when the hosts occur in different species combinations in separate ponds. One hundred fifty-three fish of the Centrarchidae, from four study sites in Nebraska, were collected. Host species included bluegill (Lepomis macrochirus), green sunfish (L. cyanellus), largemouth bass (Micropterus salmoides), black crappie (Pomoxis nigromaculatus), white crappie (P. annularis), and rock bass (Ambloplites rupestris). These fish occurred in different species combinations, depending on the pond sampled. Results indicated that several …

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Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente Feb 2003

Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente

School of Natural Resources: Faculty Publications

A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.

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Parasitism, The Diversity Of Life, And Paleoparasitology, Adauto Araújo, Ana M. Jansen, Françoise Bouchet, Karl J. Reinhard, Luiz F. Ferreira Feb 2003

Parasitism, The Diversity Of Life, And Paleoparasitology, Adauto Araújo, Ana M. Jansen, Françoise Bouchet, Karl J. Reinhard, Luiz F. Ferreira

School of Natural Resources: Faculty Publications

The parasite-host-environment system is dynamic, with several points of equilibrium. This makes it difficult to trace the thresholds between benefit and damage, and therefore, the definitions of commensalism, mutualism, and symbiosis become worthless. Therefore, the same concept of parasitism may encompass commensalism, mutualism, and symbiosis. Parasitism is essential for life. Life emerged as a consequence of parasitism at the molecular level, and intracellular parasitism created evolutive events that allowed species to diversify. An ecological and evolutive approach to the study of parasitism is presented here. Studies of the origin and evolution of parasitism have new perspectives with the development of …

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Postnatal Human Herpesvirus 8 And Human Immunodeficiency Virus Type 1 Infection In Mothers And Infants From Zambia, Brad P. Brayfield, Saul Phiri, Chipepo Kankasa, Jubra Muyanga, Hamakwa Mantina, Geoffrey Kwenda, John T. West, Ganapati Bhat, Dave B. Marx, Winslow Klaskala, Charles D. Mitchell, Charles Wood Feb 2003

Postnatal Human Herpesvirus 8 And Human Immunodeficiency Virus Type 1 Infection In Mothers And Infants From Zambia, Brad P. Brayfield, Saul Phiri, Chipepo Kankasa, Jubra Muyanga, Hamakwa Mantina, Geoffrey Kwenda, John T. West, Ganapati Bhat, Dave B. Marx, Winslow Klaskala, Charles D. Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

The specific route and timing of human herpesvirus (HHV) 8 infection in regions where Kaposi sarcoma is endemic are not known. HHV-8 infection and any risk factors that may be associated with HHV-8, including human immunodeficiency virus (HIV) type 1 infection, were monitored during the 12-month post-delivery period for 416 mothers and 485 infants from Lusaka, Zambia. HHV-8 incident infection rates during this period were 3.2 and 5.3 infections/100 person-years for infants and mothers, respectively. HHV-8 infection among infants was not associated with HHV-8 or HIV-1 infection in the mother. Among the HHV-8–positive infants, 2 of 12 tested were found …

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Bibliography Of Helminth Species Described From African Vertebrates 1800-1967, Albert G. Canaris, Scott Lyell Gardner Jan 2003

Bibliography Of Helminth Species Described From African Vertebrates 1800-1967, Albert G. Canaris, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Parasite lists, hosts lists, and bibliographies for Trematoda, Cestoda, Nematoda, and Acanthocephala.
100 pages.

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Pichia Pastoris Fermentation With Mixed-Feeds Of Glycerol And Methanol: Growth Kinetics And Production Improvement, Wenhui Zhang, Karen J. Hywood Potter, Bradley A. Plantz, Vicki L. Schlegel, Leonard A. Smith, Michael M. Meagher Jan 2003

Pichia Pastoris Fermentation With Mixed-Feeds Of Glycerol And Methanol: Growth Kinetics And Production Improvement, Wenhui Zhang, Karen J. Hywood Potter, Bradley A. Plantz, Vicki L. Schlegel, Leonard A. Smith, Michael M. Meagher

Papers in Biochemical Engineering

Fed-batch fermentation of a methanol utilization plus (Mut+) Pichia pastoris strain typically has a growth phase followed by a production phase (induction phase). In the growth phase glycerol is usually used as carbon for cell growth while in the production phase methanol serves as both inducer and carbon source for recombinant protein expression. Some researchers employed a mixed glycerol-methanol feeding strategy during the induction phase to improve production, but growth kinetics on glycerol and methanol and the interaction between them were not reported. The objective of this paper is to optimize the mixed feeding strategy based on growth kinetic studies …

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Use Of Ikonos And Landsat For Malaria Control In The Republic Of Korea, Penny M. Masuoka, David M. Claborn, Richard G. Andre, Joseph Nigro, Scott W. Gordon, Terry A. Klein, Hung-Chol Kim Jan 2003

Use Of Ikonos And Landsat For Malaria Control In The Republic Of Korea, Penny M. Masuoka, David M. Claborn, Richard G. Andre, Joseph Nigro, Scott W. Gordon, Terry A. Klein, Hung-Chol Kim

United States Army: Publications

Malaria reemerged in the Republic of Korea (ROK) in 1993. While limited numbers of U.S. soldiers in high-risk areas use chloroquine/ primaquine chemoprophylaxis to prevent malaria, control of mosquito larvae through larviciding also can be used to reduce the risk of malaria transmission. In order to estimate the cost of larviciding, accurate estimates of the spatial extent of mosquito larval habitats are necessary. The purpose of this study was to determine whether an accurate estimate of the area covered by mosquito larval habitats can be obtained using Landsat 7 Enhanced Thematic Mapper+ (ETM+) and/or IKONOS data for the Korean test …

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Generation Of An Hfrs Patient-Derived Neutralizing Recombinant Antibody To Hantaan Virus G1 Protein And Definition Of The Neutralizing Domain, Mifang Liang, Michael Mahler, Joachim Kock, Yan Ji, Dexin Li, Connie Schmaljohn, Ekkehard K.F. Bautz Jan 2003

Generation Of An Hfrs Patient-Derived Neutralizing Recombinant Antibody To Hantaan Virus G1 Protein And Definition Of The Neutralizing Domain, Mifang Liang, Michael Mahler, Joachim Kock, Yan Ji, Dexin Li, Connie Schmaljohn, Ekkehard K.F. Bautz

United States Army: Publications

Hantaan virus (HTNV) in the Hantavirus genus, family Bunyaviridae, is the major cause of severe hemorrhagic fever with renal syndrome (HFRS). We prepared a combinatorial phage display library of human Fabs to HTNV from RNA extracted from the blood lymphocytes of a convalescent HFRS patient. We selected two G1 glycoproteinspecific clones and one nucleocapsid protein (N)-specific clone from the Fab library for further studies. The human Fab antibodies were converted to IgG form in baculovirus/insect cells system by using cassette vectors that we developed earlier. Characterization of the recombinant antibodies revealed that the two G1-specific IgGs, could bind to and …

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Age-Dependent Impairment Of Igg Responses To Glycosylphosphatidylinositol With Equal Exposure To Plasmodium Falciparum Among Javanese Migrants To Papua, Indonesia, Sarah N. Hudson Keenihan, Sutanti Ratiwayanto, Saraswati Soebianto, Krisin, Harijani Marwoto, Gowdahalli Krishnegowda, D. Channe Gowda, Michael J. Bangs, David J. Frayauff, Thomas L. Richie, Sanjai Kumar, J. Kevin Baird Jan 2003

Age-Dependent Impairment Of Igg Responses To Glycosylphosphatidylinositol With Equal Exposure To Plasmodium Falciparum Among Javanese Migrants To Papua, Indonesia, Sarah N. Hudson Keenihan, Sutanti Ratiwayanto, Saraswati Soebianto, Krisin, Harijani Marwoto, Gowdahalli Krishnegowda, D. Channe Gowda, Michael J. Bangs, David J. Frayauff, Thomas L. Richie, Sanjai Kumar, J. Kevin Baird

United States Navy: Publications

Immune responses directed at glycosylphosphatidylinositol (GPI) anchors of Plasmodium falciparum may offer protection against symptomatic malaria. To independently explore the effect of age on generation of the anti-GPI IgG response, we measured serum anti-GPI IgGs in a longitudinal cohort of migrant Javanese children (6–12 years old) and adults (≥ 20 years old) with equivalent numbers of exposures to P. falciparum in Papua, Indonesia. While the peak response in adults was achieved after a single infection, comparable responses in children required ≥ 3–4 infections. Significantly fewer children (16%) than adults (41%) showed a high (optical density > 0.44) anti-GPI IgG response (odds …

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Very High Risk Of Therapeutic Failure With Chloroquine For Uncomplicated Plasmodium Falciparum And P. Vivax Malaria In Indonesian Papua, Iwa W. Sumawinata, Bernadeta, Budhi Leksana, Awalludin Sutamihardja, Purnomo, Budi Subianto, Sekartuti, David J. Fryauff, J. Kevin Baird Jan 2003

Very High Risk Of Therapeutic Failure With Chloroquine For Uncomplicated Plasmodium Falciparum And P. Vivax Malaria In Indonesian Papua, Iwa W. Sumawinata, Bernadeta, Budhi Leksana, Awalludin Sutamihardja, Purnomo, Budi Subianto, Sekartuti, David J. Fryauff, J. Kevin Baird

United States Navy: Publications

Chloroquine remains the first-line therapy for uncomplicated malaria in Indonesia. Among a series of trials of chloroquine for malaria on this archipelago conducted since 1990, we now report the highest risk of therapeutic failure yet observed. A clinical trial of standard chloroquine therapy for uncomplicated malaria at Arso PIR V in northeastern Indonesian Papua was conducted during 1995. We enrolled 104 non-immune subjects infected with Plasmodium falciparum (n=55), P. vivax (n=29), or P. falciparum plus P. vivax (n=20) and administered supervised standard chloroquine therapy (10 + 10 + 5 mg/kg at 24-hour intervals). The 28-day cumulative incidence of therapeutic failure …

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Primaquine For Prevention Of Malaria In Travelers, J. Kevin Baird, David J. Fryauff, Stephen L. Hoffman Jan 2003

Primaquine For Prevention Of Malaria In Travelers, J. Kevin Baird, David J. Fryauff, Stephen L. Hoffman

United States Navy: Publications

An expanding risk and range of endemic malaria threatens travelers. Primaquine is an old drug recently demonstrated to offer effective prophylaxis. Clinical trials conducted in Indonesia, Kenya, and Colombia showed that a primaquine base (30 mg per day) had protective efficacy against Plasmodium falciparum and Plasmodium vivax of 85%–93%. Among 339 children (age, >8 years) and adults taking this regimen for 12– 52 weeks, there was no greater risk of adverse symptomatic events among primaquine users than among recipients of placebo in double-blind studies. Among 151 subjects evaluated after 20 or 52 weeks of daily primaquine therapy, methemoglobinemia was found …

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Research At Nebraska, 2002-2003 Report, University Of Nebraska-Lincoln Jan 2003

Research At Nebraska, 2002-2003 Report, University Of Nebraska-Lincoln

Office of Research and Economic Development: Publications

This publication is a snapshot of the fiscal year’s activities. To read more stories about research and creativity at Nebraska, visit the online edition at http://research.unl.edu/news/03_ORGS_(AR_2.9.03)_Final.pdf. Additional multimedia are available for many stories.

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Ehrlichia Chaffeensis: A Prototypical Emerging Pathogen, Christopher D. Paddock, James E. Childs Jan 2003

Ehrlichia Chaffeensis: A Prototypical Emerging Pathogen, Christopher D. Paddock, James E. Childs

Zoonotics and Wildlife Disease: Publications

In April 1986, a medical intern scanning the peripheral blood smear of a severely ill man with an unexplained illness observed peculiar intracytoplasmic inclusions in several of the patient’s monocytes. The patient described multiple tick bites sustained approximately 2 weeks earlier during a visit to a rural area in northern Arkansas, and a presumptive diagnosis of Rocky Mountain spotted fever had been made (104, 174). Clinicians and scientists subsequently identified these inclusions as clusters of bacteria belonging to the genus Ehrlichia, previously known in the United States solely as veterinary pathogens (174). Within the next 5 years, the organism …

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Cd21-Positive Follicular Dendritic Cells A Possible Source Of PrpSc In Lymph Node Macrophages Of Scrapie-Infected Sheep, Lynn M. Herrmann, William P. Cheevers, William C. Davis, Donald P. Knowles, Katherine I. O'Rourke Jan 2003

Cd21-Positive Follicular Dendritic Cells A Possible Source Of PrpSc In Lymph Node Macrophages Of Scrapie-Infected Sheep, Lynn M. Herrmann, William P. Cheevers, William C. Davis, Donald P. Knowles, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Natural sheep scrapie is a prion disease characterized by the accumulation of PrPSc in brain and lymphoid tissues. Previous studies suggested that lymph node macrophages and follicular dendritic cells (FDC) accumulate PrPSc. In this study, lymph nodes were analyzed for the presence of PrPSc and macrophage or FDC markers using dual immunohistochemistry. A monoclonal antibody (mAb) to the C-terminus of PrP reacted with CD172a+ macrophages and CD21+ FDC processes in secondary follicles. However, a PrP N-terminus- specific mAb reacted with CD21+ FDC processes but not CD172a+ macrophages in secondary follicles. Neither the PrP N-terminus nor C-terminus-specific …

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Immunohistochemical Detection And Distribution Of Prion Protein In A Goat With Natural Scrapie, Reginald A. Valdez, Matthew J. Rock, Anne K. Anderson, Katherine I. O'Rourke Jan 2003

Immunohistochemical Detection And Distribution Of Prion Protein In A Goat With Natural Scrapie, Reginald A. Valdez, Matthew J. Rock, Anne K. Anderson, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Formalin-fixed, paraffin-embedded tissue sections from a 3-year-old female Angora goat suffering from clinical scrapie were immunostained after hydrated autoclaving using a monoclonal antibody (mAb, F99/ 97.6.1; IgG1) specific for a conserved epitope on the prion protein. Widespread and prominent deposition of the scrapie isoform of the prion protein (PrPSc) was observed in the brain, brainstem, spinal cord, retina, postganglionic neurons associated with parasympathetic ganglia of myenteric and submucosal plexuses, Peyer’s patches, peripheral lymph nodes, and pharyngeal and palatine tonsils. The goat was homozygous for PrP alleles encoding 5 octapeptide repeat sequences in the N-terminal region of the prion …

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A Prion Protein Epitope Selective For The Pathologically Misfolded Conformation, Eustache Paramithiotis, Marc Pinard, Trebor Lawton, Sylvie Laboissiere, Valerie I. Leathers, Wen-Quan Zou, Lisa A. Estey, Julie Lamontagne, Marty T. Lehto, Leslie H. Kondejewski, Gregory P. Francoeur, Maria Papadopoulos, Ashkan Haghighat, Stephen J. Spatz, Mark Head, Robert Will, James Ironside, Katherine I. O'Rourke, Quentin Tonelli, Harry C. Ledebur, Avi Chakrabartty, Neil R. Cashman Jan 2003

A Prion Protein Epitope Selective For The Pathologically Misfolded Conformation, Eustache Paramithiotis, Marc Pinard, Trebor Lawton, Sylvie Laboissiere, Valerie I. Leathers, Wen-Quan Zou, Lisa A. Estey, Julie Lamontagne, Marty T. Lehto, Leslie H. Kondejewski, Gregory P. Francoeur, Maria Papadopoulos, Ashkan Haghighat, Stephen J. Spatz, Mark Head, Robert Will, James Ironside, Katherine I. O'Rourke, Quentin Tonelli, Harry C. Ledebur, Avi Chakrabartty, Neil R. Cashman

Zoonotics and Wildlife Disease: Publications

Conformational conversion of proteins in disease is likely to be accompanied by molecular surface exposure of previously sequestered amino-acid side chains. We found that induction of β-sheet structures in recombinant prion proteins is associated with increased solvent accessibility of tyrosine. Antibodies directed against the prion protein repeat motif, tyrosine-tyrosinearginine, recognize the pathological isoform of the prion protein but not the normal cellular isoform, as assessed by immunoprecipitation, plate capture immunoassay and flow cytometry. Antibody binding to the pathological epitope is saturable and specific, and can be created in vitro by partial denaturation of normal brain prion protein. Conformation-selective exposure of …

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Properties Of A Disease-Specific Prion Probe, Guy Gorochov, Jean-Phillippe Deslys, Eustache Paramithiotis, Marc Pinard, Trebor Lawton, Sylvie Laboissiere, Valerie L. Leathers, Wen-Quan Zou, Lisa A. Estey, Julie Lamontagne, Marty T. Lehto, Leslie H. Kondejewski, Gregory P. Francoeur, Maria Paoadopoulos, Ashkan Haghighat, Stephen J. Spatz, Mark Head, Robert Will, James Ironside, Katherine I. O'Rourke, Quentin Tonelli, Harry C. Ledebur, Avi Chakrabartty, Neil R. Cashman Jan 2003

Properties Of A Disease-Specific Prion Probe, Guy Gorochov, Jean-Phillippe Deslys, Eustache Paramithiotis, Marc Pinard, Trebor Lawton, Sylvie Laboissiere, Valerie L. Leathers, Wen-Quan Zou, Lisa A. Estey, Julie Lamontagne, Marty T. Lehto, Leslie H. Kondejewski, Gregory P. Francoeur, Maria Paoadopoulos, Ashkan Haghighat, Stephen J. Spatz, Mark Head, Robert Will, James Ironside, Katherine I. O'Rourke, Quentin Tonelli, Harry C. Ledebur, Avi Chakrabartty, Neil R. Cashman

Zoonotics and Wildlife Disease: Publications

In a recently published article, Paramithiotis et al. describe antibodies specific for the prion Tyr-Tyr-Arg (YYR) repeat motif. These antibodies interact with the pathological isoform of the prion protein (PrPSC), but not with the normal cellular isoform (PrpC). Because of this restricted specificity, they suggest that YYR-specific antibodies could be useful for the diagnosis and treatment of prion diseases (Fig. O. The monoclonal antibodies, all of the IgM isotype, were produced by immunizing mice with a synthetic peptide (CYYRRYYRYY). When coupled to magnetic beads, these YYR-specific antibodies immunoprecipitate Prpsc much more efficiently than PrpC …

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Rapid Communication Rescue Of Hantaan Virus Minigenomes, Kirsten Flick, Connie S. Schmaljohn, Ralf F. Petterson, Heinz Feldmann, Ramon Flick, Jay Hooper Jan 2003

Rapid Communication Rescue Of Hantaan Virus Minigenomes, Kirsten Flick, Connie S. Schmaljohn, Ralf F. Petterson, Heinz Feldmann, Ramon Flick, Jay Hooper

United States Army: Publications

Hantavirus infections are a major public health concern worldwide. Their widespread geographical distribution and their ability to produce serious, often fatal, human disease underline the need for a system that allows manipulation of these viruses. We describe here the first successful establishment of a reverse genetics technology for Hantaan virus, the prototype of the genus Hantavirus. The system offers a unique opportunity to study the biology of hantaviruses, the pathogenesis of the diseases, and the efficacy of antiviral and prophylactic measures against hantavirus infections.

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2003 Trafficking In Persons Report, U.S. Department Of State Jan 2003

2003 Trafficking In Persons Report, U.S. Department Of State

Human Trafficking: Data and Documents

AS unimaginable as it seems, slavery and bondage still persist in the early twenty-first century. Millions of people around the world still suffer in silence in slave-like situations of forced labor and commercial sexual exploitation from which they cannot free themselves. Trafficking in persons is one of the greatest human rights challenges of our time. It is, as the International Labour Organization (ILO) points out, the “underside of globalization.”

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Monte Carlo Assessments Of Goodness-Of-Fit For Ecological Simulation Models, Lance A. Waller, David Smith, James E. Childs, Leslie A. Real Jan 2003

Monte Carlo Assessments Of Goodness-Of-Fit For Ecological Simulation Models, Lance A. Waller, David Smith, James E. Childs, Leslie A. Real

Zoonotics and Wildlife Disease: Publications

One often develops stochastic ecologic simulation models based on local interactions between individuals or groups and bases systemic conclusions on trends summarized over multiple data sets generated from the model. In many cases, such models generate data sets (“realizations”) each violating the usual assumptions associated with traditional statistical tests of goodness-of-fit, most notably that of independent observations. Monte Carlo hypothesis tests applied to multiple realizations from such models provide appropriate goodness-of-fit tests regardless of within-model peculiarities. The Monte Carlo tests address the question “Do the observed data appear consistent with the model?” in contrast to the usual question “Does the …

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Abundant PrpCwd In Tonsil From Mule Deer With Preclinical Chronic Wasting Disease, Katherine I. O'Rourke, Dongyue Zhuang, Amy Lyda, Gabriel Gomez, Elizabeth S. Williams, Wenbin Tuo, Michael W. Miller Jan 2003

Abundant PrpCwd In Tonsil From Mule Deer With Preclinical Chronic Wasting Disease, Katherine I. O'Rourke, Dongyue Zhuang, Amy Lyda, Gabriel Gomez, Elizabeth S. Williams, Wenbin Tuo, Michael W. Miller

Zoonotics and Wildlife Disease: Publications

A monoclonal antibody dot-blot assay was used to evaluate detergent lysates of tonsil tissue from mule deer to detect PrPCWD, the marker for the cervid transmissible spongiform encephalopathy chronic wasting disease (CWD). Samples of formalin-fixed brain and tonsil tissues from mule deer were examined for PrPCWD using immunohistochemistry (IHC) with Mab F99/97.6.1, the gold standard for diagnosis of preclinical CWD. The contralateral tonsil from each of the 143 deer was prepared for confirmatory IHC and as a 10% (wt/vol) detergent lysate without purification or enrichment steps for monoclonal antibody dot-blot assay. PrPCWD was detected by dot-blot …

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Comparison Of The Protective Efficacy Of Dna And Baculovirusderived Protein Vaccines For Ebola Virus In Guinea Pigs, Jenny Mellquist-Riemenschneider, Aura Garrison, Joan Geisbert, Kamal Saikh, Kelli Heidebrink, Peter Jahrling, Robert Ulrich, Connie Schmaljohn Jan 2003

Comparison Of The Protective Efficacy Of Dna And Baculovirusderived Protein Vaccines For Ebola Virus In Guinea Pigs, Jenny Mellquist-Riemenschneider, Aura Garrison, Joan Geisbert, Kamal Saikh, Kelli Heidebrink, Peter Jahrling, Robert Ulrich, Connie Schmaljohn

United States Army: Publications

The filoviruses Ebola virus (EBOV) and Marburg virus (MARV) cause severe hemorrhagic fever in humans for which no vaccines are available. Previously, a priming dose of a DNA vaccine expressing the glycoprotein (GP) gene of MARV followed by boosting with recombinant baculovirus-derived GP protein was found to confer protective immunity to guinea pigs (Hevey et al., 2001. Vaccine 20, 568-593). To determine whether a similar prime-boost vaccine approach would be effective for EBOV, we generated and characterized recombinant baculoviruses expressing full-length EBOV GP (GP1,2) or a terminally-deleted GP (GPa-) and examined their immunogenicity in guinea pigs. As expected, …

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A Priori Prediction Of Disease Invasion Dynamics In A Novel Environment, Colin A. Russell, David L. Smith, Lance A. Waller, James E. Childs, Leslie A. Real Jan 2003

A Priori Prediction Of Disease Invasion Dynamics In A Novel Environment, Colin A. Russell, David L. Smith, Lance A. Waller, James E. Childs, Leslie A. Real

Zoonotics and Wildlife Disease: Publications

Directly transmitted infectious diseases spread through wildlife populations as travelling waves away from the sites of original introduction. These waves often become distorted through their interaction with environmental and population heterogeneities and by long-distance translocation of infected individuals. Accurate a priori predictions of travelling waves of infection depend upon understanding and quantifying these distorting factors. We assess the effects of anisotropies arising from the orientation of rivers in relation to the direction of disease-front propagation and the damming effect of mountains on disease movement in natural populations. The model successfully predicts the local and large-scale prevaccination spread of raccoon rabies …

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Office Of Research And Graduate Studies -- Annual Report 2002/2003 Jan 2003

Office Of Research And Graduate Studies -- Annual Report 2002/2003

Office of Research and Economic Development: Publications

Contents
01 Introduction
03 Nanomagnetics & Mesospin Structures
05 Redox Biology
07 Virology & Infectious Diseases
09 Bioprocessing & Vaccine Development
11 Plant Genomics
13 Complex Environmental Systems
15 Behavioral Sciences
17 Education in the 21st Century
19 Digital Texts & Contemporary Writing
21 Nanomanufacturing & Advanced Materials
23 Initiatives
25 Future
27 Graduate Studies
29 Technology Development
31 University of Nebraska Press
32 Financials
33 Inspiring Excellence

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