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The Incidence Of Genotypes At Codon 171 Of The Prion Protein Gene (Prnp) In Five Breeds Of Sheep And Production Traits Of Ewes Associated With Those Genotypes, B. M. Alexander, R. H. Stobart, W. C. Russell, Katherine I. O'Rourke, G. S. Lewis, J. R. Logan, J. V. Duncan, G. E. Moss

Zoonotics and Wildlife Disease: Publications

Scrapie is one of several transmissible spongiform encephalopathies of livestock. Disease susceptibility is linked to polymorphisms in the normal prion protein gene that encodes the mammalian prion precursor. Codon 171 of this gene is a major determinant of scrapie susceptibility. Selection for arginine (R) at codon 171 is encouraged by the USDA to decrease the incidence of scrapie. Objectives of this study were to determine the frequency of R allele variants at codon 171 in a sample of sheep from five breeds (Columbia, Hampshire, Rambouillet, Suffolk, and Targhee) and western white-faced commercial ewes and to determine whether the R allele …

Chronic Wasting Disease Of Elk: Transmissibility To Humans Examined By Transgenic Mouse Models, Qingzhong Kong, Shenghai Huang, Wenquan Zou, Difernando Vanegas, Meiling Wang, Di Wu, Jue Yuan, Mengjie Zheng, Hua Bai, Huayun Deng, Ken Chen, Allen L. Jenny, Katherine I. O'Rourke, Ermais D. Belay, Lawrence B. Schonberger, Robert B. Pertersen, Man-Sun Sy, Shu G. Chen, Pierluigi Gambetti

Zoonotics and Wildlife Disease: Publications

Chronic wasting disease (CWD), a prion disease affecting free-ranging and captive cervids (deer and elk), is widespread in the United States and parts of Canada. The large cervid population, the popularity of venison consumption, and the apparent spread of the CWD epidemic are likely resulting in increased human exposure to CWD in the United States. Whether CWDis transmissible to humans, as has been shown for bovine spongiform encephalopathy (the prion disease of cattle), is unknown. We generated transgenic mice expressing the elk or human prion protein (PrP) in a PrP-null background. After intracerebral inoculation with elk CWD prion, two lines …

Conformational Sampling Of The Botulinum Neurotoxin Serotype A Light Chain: Implications For Inhibitor Binding, James C. Burnett, James J. Schmidt, Connor F. Mcgrath, Tam L. Nguyen, Ann R. Hermone, Rekha G. Panchal, Jonathan L. Vennerstrom, Krishna Kodukula, Daniel W. Zaharevitz, Rick Gussio, Sina Bavari

United States Army: Publications

Botulinum neurotoxins (BoNTs) are the most potent of the known biological toxins, and consequently are listed as category A biowarfare agents. Currently, the only treatments against BoNTs include preventative antitoxins and long-term supportive care. Consequently, there is an urgent need for therapeutics to counter these enzymes––post exposure. In a previous study, we identified a number of small, nonpeptidic lead inhibitors of BoNT serotype A light chain (BoNT/A LC) metalloprotease activity, and we identified a common pharmacophore for these molecules. In this study, we have focused on how the dynamic movement of amino acid residues in and surrounding the substrate binding …

Support For Victims Of Human Trafficking, U.S. Department Of Health And Human Services

Human Trafficking: Data and Documents

Preventive health care for victims of human trafficking is virtually non-existent, and health problems are typically not treated in their early stages.

Predictive Spatial Dynamics And Strategic Planning For Raccoon Rabies Emergence In Ohio, James E. Childs, David L. Smith, Colin A. Russell, Leslie A. Real

Zoonotics and Wildlife Disease: Publications

Rabies is an important public health concern in North America because of recent epidemics of a rabies virus variant associated with raccoons. The costs associated with surveillance, diagnostic testing, and post-exposure treatment of humans exposed to rabies have fostered coordinated efforts to control rabies spread by distributing an oral rabies vaccine to wild raccoons. Authorities have tried to contain westward expansion of the epidemic front of raccoonassociated rabies via a vaccine corridor established in counties of eastern Ohio, western Pennsylvania, and West Virginia. Although sporadic cases of rabies have been identified in Ohio since oral rabies vaccine distribution in 1998, …

Experimental Transmission Of Chronic Wasting Disease Agent From Mule Deer To Cattle By The Intracerebral Route, Amir N. Hamir, Robert A. Kunkle, Randall C. Cutlip, Janice M. Miller, Katherine I. O'Rourke, Elizabeth S. Williams, Michael W. Miller, Mick J. Stack, Melanie J. Chaplin, Jügen A. Richt

Zoonotics and Wildlife Disease: Publications

This communication reports final observations on experimental transmission of chronic wasting disease (CWD) from mule deer to cattle by the intracerebral route. Thirteen calves were inoculated intracerebrally with brain suspension from mule deer naturally affected with CWD. Three other calves were kept as uninoculated controls. The experiment was terminated 6 years after inoculation. During that time, abnormal prion protein (PrP^res) was demonstrated in the central nervous system (CNS) of 5 cattle by both immunohistochemistry and Western blot. However, microscopic lesions suggestive of spongiform encephalopathy (SE) in the brains of these PrP^res-positive animals were subtle in 3 …

Assessment Of Texas Medical Providers Concerning Rabies Vaccines, Denise Ruffino, Scott Henke

Wildlife Damage Management Conference Proceedings

Rabies is an important zoonotic disease in Texas and thousands of people each year either request or require rabies prophylaxis because they have ‘high risk’ jobs or are exposed to the disease. After experiencing difficulty in receiving rabies prophylaxis from physicians, we conducted a survey of Texas medical providers to assess their knowledge of rabies vaccine procedures and their experience with rabies vaccines. Most providers in Texas (>95% of 297) rarely saw patients for rabies prophylaxis; therefore, providers have minimal, if any, experience with the procedures of acquiring and administering the vaccine. Providers varied greatly in their responses to …

Challenges Of Invasive Reptiles And Amphibians, William Pitt, Daniel Vice, Mike Pitzler

Wildlife Damage Management Conference Proceedings

Although worldwide distributions of many amphibians and reptiles are declining, a handful of species are spreading rapidly throughout tropical regions of the world. The species that have the greatest effect tend to be generalist feeders, have high reproductive rates, attain large population sizes, and often due to their behavior and or small size, are easily transported or are difficult to detect. The most notable of these species include the coqui frog, cane toad, bullfrog, brown tree snake, and Burmese pythons. The effect of a few individuals typically is small but the combined effect of large populations can be devastating to …

Preliminary Serologic Survey Of Selected Diseases And Movements Of Feral Swine In Texas, A. Christy Wykoff, Scott Henke, Tyler Campbell, David G. Hewitt, Kurt C. Vercauteren

Wildlife Damage Management Conference Proceedings

Feral swine (Sus scrofa) populations occur throughout eastern, central, and southern Texas, and their populations appear to be increasing. Despite their abundance and wide distribution, little is known about their range and interaction with domestic animals. In the last decade the national pork production industry has enforced an eradication program for economically detrimental swine diseases such as pseudorabies and brucellosis. It is hypothesized that feral hogs can be reservoirs that could reintroduce diseases to disease-free domestic swine herds. The objectives of this on-going project are to determine the prevalence of selected swine diseases that exist within feral hog …

Oral Rabies Vaccination—A Progress Report, Dennis Slate, Charles Rupprecht, Mike Dunbar, Robert Mclean

Wildlife Damage Management Conference Proceedings

Oral rabies vaccination (ORV) targeting specific wild Carnivora species has emerged as an integral adjunct to conventional rabies control strategies to protect humans and domestic animals. ORV has been applied with progress toward eliminating rabies in red foxes (Vulpes vulpes) in western Europe and southern Ontario, Canada. Beginning in the 1990’s, coordinated ORV was implemented in Texas to contain and eliminate variants of rabies virus in the gray fox (Urocyon cinereoargenteus ) and coyote (Canis latrans ) and in several eastern U.S. States with the goal of preventing spread of raccoon (Procyon lotor ) rabies. …

Compressed Air, Wooden Clappers, And Other Non-Traditional Methods For Dispersing European Starlings From An Urban Roost, Randolph White, Richard Dolbeer, Craig Hicks

Wildlife Damage Management Conference Proceedings

During autumn 2003, several thousand European starlings (Sturnus vulgaris) began roosting on exposed I-beams in a newly constructed, decorative glass canopy that covered the passenger pick-up area at the terminal building for Cleveland Hopkins International Airport, Ohio. The use of lethal control or conventional dispersal techniques, such as pyrotechnics and fire hoses, were not feasible in the airport terminal area. The design and aesthetics of the structure precluded the use of netting and other exclusion materials. In January 2004, an attempt was made to disperse the birds using recorded predator and distress calls broadcast from speakers installed in …

Department Of Veterinary And Biomedical Sciences: 2005 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Veterinary and Biomedical Sciences Personnel
VBMS Teaching Program - Courses 2005
VBMS Grants and Contracts Program, 2005
Publications by VBMS Faculty in 2005
Selected Committees, Editorial and Other Appointments. 2005
Articles Regarding the Department in 2005
Departmental Budget Summaries, 2005
Nebraska Agricultural Statistics, 2004/2005.

Genome Size And Organization In The Blacklegged Tick, Ixodes Scapularis And The Southern Cattle Tick, Boophilus Microplus, A. J. Ullmann, C. M. R. Lima, F. D. Guerrero, J. Piesman, W. C. Black Iv

United States Public Health Resources

Genome sizes and the organization of repetitive DNA were determined in the hard ticks Ixodes scapularis and Boophilus microplus using reassociation kinetics. The I. scapularis genome contains ~ 2.15 pg (2.1 × 10 ³ Mbp) of DNA and consists of no foldback (FB), 27% highly repetitive (HR), 39% moderately repetitive (MR), and 34% unique DNA. The B. microplus genome contains 7.5 pg (7.1 × 10 ³ Mbp) DNA, and consists of 0.82% FB, 31% HR, 38% MR, and 30% unique DNA. In both species, repetitive sequences occur in a mixture of long and short period interspersion but most (65–80%) of …

Evolutionary Genetics Of A New Pathogenic Escherichia Species: Escherichia Albertii And Related Shigella Boydii Strains, Katie E. Hyma, David W. Lacher, Adam M. Nelson, Alyssa C. Bumbaugh, J. Michael Janda, Nancy A. Strockbine, Vincent B. Young, Thomas S. Whittam

United States Public Health Resources

A bacterium originally described as Hafnia alvei induces diarrhea in rabbits and causes epithelial damage similar to the attachment and effacement associated with enteropathogenic Escherichia coli. Subsequent studies identified similar H. alvei-like strains that are positive for an intimin gene (eae) probe and, based on DNA relatedness, are classified as a distinct Escherichia species, Escherichia albertii. We determined sequences for multiple housekeeping genes in five E. albertii strains and compared these sequences to those of strains representing the major groups of pathogenic E. coli and Shigella. A comparison of 2,484 codon positions in 14 …

Non-O157 Shiga Toxin–Producing Escherichia Coli Infections In The United States, 1983–2002, John T. Brooks, Evangeline G. Sowers, Joy G. Wells, Katherine D. Greene Greene, Patricia M. Griffin, Robert M. Hoekstra, Nancy A. Strockbine

United States Public Health Resources

Background. Shiga toxin–producing Escherichia coli (STEC) O157:H7 is a well-recognized cause of bloody diarrhea and hemolytic-uremic syndrome (HUS). Non-O157 STEC contribute to this burden of illness but have been underrecognized as a result of diagnostic limitations and inadequate surveillance.

Methods. Between 1983 and 2002, 43 state public health laboratories submitted 940 human non-O157 STEC isolates from persons with sporadic illnesses to the Centers for Diseases Control and Prevention reference laboratory for confirmation and serotyping.

Results. The most common serogroups were O26 (22%), O111 (16%), O103 (12%), O121 (8%), O45 (7%), and O145 (5%). Non-O157 STEC infections were …

Identification And Characterization Of Shigella Boydii 20 Serovar Nov., A New And Emerging Shigella Serotype, David L. Woodward, Clifford G. Clark, Richard A. Caldeira, Rafiq Ahmed, Geoff Soule, Louis Bryden, Helen Tabor, Pasquale Melito, Roger Foster, Julie Walsh, Lai-King Ng, Georgia B. Malcolm, Nancy Strockbine, Frank G. Rodgers

United States Public Health Resources

Analysis of 163 putative Shigella isolates from Canada and the USA showed biochemical reactions consistent with Shigella species, although none of the isolates reacted with antiserum raised against any of the well-established or provisional Shigella serotypes. All these isolates, provisionally designated serotype SH108, were positive for the ipaH gene and the invasion-associated locus. All fermented mannitol, were serologically indistinguishable from each other and showed no reaction in antisera prepared against Escherichia coli serotypes O1 to O181. PCR-RFLP analysis of the genes involved in O-antigen synthesis revealed a common pattern among these isolates that was distinct from recognized Shigella serotypes and …

Phylogenetic Analysis Of North American West Nile Virus Isolates, 2001–2004: Evidence For The Emergence Of A Dominant Genotype, C. Todd Davis, Gregory D. Ebel, Robert S. Lanciotti, Aaron C. Brault, Hilda Guzman, Marina Siirin, Amy Lambert, Ray E. Parsons, David W.C. Beasley, Robert J. Novak, Darwin Elizondo-Quiroga, Emily N. Green, David S. Young, Lillian M. Stark, Michael A. Drebot, Harvey Artsob, Robert B. Tesh, Laura D. Kramer, Alan D.T. Barrett

United States Public Health Resources

The distribution of West Nile virus has expanded in the past 6 years to include the 48 contiguous United States and seven Canadian provinces, as well as Mexico, the Caribbean islands, and Colombia. The suggestion of the emergence of a dominant genetic variant has led to an intensive analysis of isolates made across North America. We have sequenced the premembrane and envelope genes of 74 isolates and the complete genomes of 25 isolates in order to determine if a dominant genotype has arisen and to better understand how the virus has evolved as its distribution has expanded. Phylogenetic analyses revealed …

Effect Of Cirrhosis On Antibiotic Efficacy In A Rat Model Of Pneumococcal Pneumonia, Laurel C. Preheim, Keith M. Olsen, Mei Yue, Mary U. Snitily, Martha J. Gentry-Nielsen

United States Public Health Resources

A rat model was used to study the effects of cirrhosis on antibiotic therapy of pneumococcal pneumonia. Cirrhotic and control male Sprague-Dawley rats were infected transtracheally with type 3 Streptococcus pneumonia. Treatment began 18 h later with phosphate-buffered saline (PBS), azithromycin (50 mg/kg), trovafloxacin (50 mg/kg), or ceftriaxone (100 mg/kg) injected subcutaneously twice daily for 5 days. Antibiotic concentrations were measured by high-performance liquid chromatography. Azithromycin, trovafloxacin, and ceftriaxone were all equally effective at preventing mortality in both cirrhotic and normal rats. Free fraction area under the curve to minimum inhibitory concentration ratio (AUC/MIC) and maximum calculated serum concentration …

Spatial Management Of Wildlife Disease, Richard Horan, Christopher Wolf, Eli P. Fenichel, Kenneth H. Mathews, Jr.

Michigan Bovine Tuberculosis Bibliography and Database

The spread of wildlife diseases is a major threat to livestock, human health, resource-based recreation, and biodiversity conservation (Cleaveland, Laurenson, and Taylor). The development of economically sound wildlife disease-management strategies requires an understanding of the links between ecological functions (e.g., disease transmission and wildlife dispersal) and economic choices, and the associated tradeoffs. Spatial linkages are particularly relevant. Yet while ecologists have long-argued that space is important (Hudson et al.), prior economic work has largely ignored spatial issues.

For instance, Horan and Wolf analyzed a case study of bovine tuberculosis (bTB) in Michigan deer, a problem where the disease appears to …

Michigan Bovine Tuberculosis Eradication Project: 2005 Activities Report And Conference Proceedings

Michigan Bovine Tuberculosis Bibliography and Database

On June 7 and 8, 2005, the State of Michigan and U.S. Department of Agriculture hosted the ninth annual bovine Tuberculosis (TB) meeting of scientists, with the intent to share research information and provide updates on policies, regulations and activities regarding bovine TB.

This document is a thank you to stakeholders and serves as an annual report offering insight into the finer points of this multi-agency project. The year 2005 has been one of milestones. The Upper Peninsula received TB-Free Status; only one TB positive farm was found during routine surveillance testing; and the disease prevalence rate in Deer Management …

Fence-Line Interactions Among Farmed And Free-Ranging Cervids: Preliminary Results, Kurt C. Vercauteren, Justin Fischer, Robert Pooler, Michael J. Lavelle, Greg Phillips

Wildlife Damage Management Conference Proceedings

Occurrences of disease outbreaks within and near captive cervid (mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginianus), and Rocky Mountain elk (Cervus elaphus nelsoni)) farms have recently drawn attention to these facilities. Some state wildlife and agricultural agencies have pondered making double fencing mandatory or otherwise increasing regulation of captive operations. Diseases such as Chronic Wasting Disease (CWD) and Bovine Tuberculosis (bovine TB) are a threat to captive and free-ranging cervid populations. Concerns over these and other diseases being transmitted from captive to wild cervids, and vice versa, have increased. In most cases, captive …

Scwds Briefs: Volume 20, Number 4 (January 2005), Michael J. Yabsley

Southeastern Cooperative Wildlife Disease Study: Publications

Dr. Tom Thorne, a 36-year veteran of the Wyoming Game and Fish Department, and his wife, Dr. Beth Williams, a professor of pathology at the University of Wyoming, were killed in a tragic automobile accident on December 29, 2004.
Dr. Al Kocan, recently retired professor of parasitology from Oklahoma State University, died of an apparent heart attack on December 15, 2004.
SCWDS Studies on Johne’s Disease in Wildlife Johne’s disease is a chronic, progressive disease of the gastrointestinal tract and lymphoid tissue that is caused by the bacterium Mycobacterium avium subspecies paratuberculosis (Map). Johne’s disease is known primarily as a …

Potential Roles Of Follicular Dendritic Cell–Associated Osteopontin In Lymphoid Follicle Pathology And Repair And In B Cell Regulation In Hiv-1 And Siv Infection, Qingsheng Li, Jeffrey D. Lifson, Lijie Duan, Timothy Schacker, Cavan Reilly, John V. Carlis, Jacob D. Estes, Ashley T. Haase

Qingsheng Li Publications

Osteopontin is a multifunctional protein with known roles in bone remodeling, wound healing, and normal and pathological immune responses. We showed in microarray studies that osteopontin gene expression is increased in human immunodeficiency virus type 1 (HIV-1)–infected lymphatic tissues after treatment, and we undertook mapping experiments to study osteopontin’s possible functions in this context. We discovered species-specific colocalization of osteopontin with the follicular dendritic cell (FDC) network in lymphatic tissues in HIV-1 and simian immunodeficiency virus infections, and we found that changes in FDC-associated osteopontin covary with changes in lymphoid follicles during acute and late stages of infection and in …

Breeding Behavior Of The Mongolian Pika (Ochotona Pallasi) In The Gobi Gurvan Saykhan Mountains, Mongolia, Tserendorjiin Munkhzul

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Behavior, morphology, and biology of the Mongolian pika (Ochotona pallasi) were investigated within the framework of the joint ’Grazing Research Project’ of the Philipps-University of Mar-burg, Germany and the Mongolian National University in Ulaanbaatar. The study took place in the Stipa-Allium-steppes of the upper pediments in the Dund Saykhan mountain of the Gobi Gurvan Saykhan National Park, where its burrows cover a significant part of the area.

The behavior of the Mongolian pikas changes seasonally. During the rut in spring males are generally more occupied with defending their territory and fighting off rivals, while females spend more time …

Overview Of Impacts Of Feral And Introduced Ungulates On The Environment In The Eastern United States And Caribbean, Martin Lowney, Paul Schoenfeld, William Haglan, Gary Witmer

Wildlife Damage Management Conference Proceedings

Non-native wild and feral ungulates have been introduced throughout the world for many centuries. Often the reasons for introductions were narrow in scope and benefits or the ungulates escaped or were released. Justifications for some introductions have included providing hunting opportunity, meeting cultural and dietary needs of people, fund raising, and aesthetics. Evaluations about the impacts to the environment, native wildlife, livestock, and people were most likely looked at in a narrow prism or not fully evaluated. Ungulates commonly introduced in the Eastern United States and Caribbean islands over the last 150 years included white-tailed deer, sika deer, hogs, horses, …

Monitoring Raccoon Rabies In Alabama: The Potential Effects Of Habitat And Demographics, Wendy Arjo, Christine Fisher, James Armstrong, Dana Johnson, Frank Boyd

Wildlife Damage Management Conference Proceedings

Density, morphometrics, and disease prevalence of raccoon populations were determined in 4 habitats (agriculture, riverine, managed, and forested) in central Alabama. In addition we monitored 71 collared raccoons to determine survival. Density estimates were similar in the agriculture (ag) and riverine habitats in central Alabama with 8 raccoons/km², and lower in the forested habitat at 5 raccoons/ km². Retention of juveniles did not appear to contribute to observed higher populations in the riverine and ag habitat. Although the riverine and ag, possibly due to supplemental resources, likely provide better habitat for raccoons, we found only body …

Morbidity And Mortality Factors In Pre-Fledged Florida Sandhill Crane (Grus Canadensis Pratensis) Chicks, Robert J. Dusek, Marilyn G. Spalding, Donald J. Forrester, Nicholas Komar, Jonathan F. Day

Proceedings of the North American Crane Workshop

One hundred and fifteen Florida sandhill crane (Grus canadensis pratensis) chicks were captured in Osceola and Lake Counties, Florida in 1998 - 2000 and examined for evidence of disease. Evidence of Eimeria gruis and/or E. reichenowi infection was found in 52% of chicks examined. Ten chicks were positive for antibodies to St. Louis encephalitis virus and 1 of these chicks was also positive for antibodies to eastern equine encephalitis virus. Predation was the most commonly identified cause of mortality. An unidentified microfilaria, and an unknown protozoan were detected in blood smears from crane chicks. A number of other …

Molecular Characterization Of Mycobacterium Tuberculosis Complex Isolates From Wild Ungulates In South-Central Spain, Christian Gortazar, Joaquín Vicente, Sofia Samper, Joseba M. Garrido, Isabel Fernandez-De-Mera, Patricia Gavín, Ramón A. Juste, Carlos Martín, Pelayo Acevedo, Manuel De La Puentea, Ursula Höflea

Michigan Bovine Tuberculosis Bibliography and Database

The role of European wild ungulates in the epidemiology of tuberculosis (TB) is still under discussion. This study describes the geographical distribution and molecular typing of 77 Mycobacterium tuberculosis complex isolates belonging either to M. bovis or to M. caprae, cultivated from hunter harvested red deer (Cervus elaphus) and European wild boar (Sus scrofa) in 24 Spanish localities, and compares them with spoligotypes detected previously in humans, livestock or wild animals, as described in the literature. The distribution of the molecular type patterns suggests that the population of M. tuberculosis complex strains isolated from Spanish …

Use Of An Electronic Nose To Diagnose Mycobacterium Bovis Infection In Badgers And Cattle, R. Fend, R. Geddes, S. Lesellier, H.-M. Vordermeier, L. A. L. Corner, E. Gormley, E. Costello, R. G. Hewinson, D. J. Marlin, A. C. Woodman, M. A. Chambers

Michigan Bovine Tuberculosis Bibliography and Database

It is estimated that more than 50 million cattle are infected with Mycobacterium bovis worldwide, resulting in severe economic losses. Current diagnosis of tuberculosis (TB) in cattle relies on tuberculin skin testing, and when combined with the slaughter of test-positive animals, it has significantly reduced the incidence of bovine TB. The failure to eradicate bovine TB in Great Britain has been attributed in part to a reservoir of the infection in badgers (Meles meles). Accurate and reliable diagnosis of infection is the cornerstone of TB control. Bacteriological diagnosis has these characteristics, but only with samples collected postmortem. Unlike …

Hidden Effects Of Chronic Tuberculosis In African Buffalo, Anna E. Jolles, David V. Cooper, Simon A. Levin

Michigan Bovine Tuberculosis Bibliography and Database

Infectious diseases can bring about population declines and local host extinctions, contributing significantly to the global biodiversity crisis. Nonetheless, studies measuring population-level effects of pathogens in wild host populations are rare, and taxonomically biased toward avian hosts and macroparasitic infections. We investigated the effects of bovine tuberculosis (bTB), caused by the bacterial pathogen Mycobacterium bovis, on African buffalo (Syncerus caffer) at Hluhluwe-iMfolozi Park, South Africa. We tested 1180 buffalo for bTB infection between May 2000 and November 2001. Most infections were mild, confirming the chronic nature of the disease in buffalo. However, our data indicate that bTB …