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Immune Evasion Of Porcine Reproductive And Respiratory Syndrome Virus Through Glycan Shielding Involves Both Glycoprotein 5 As Well As Glycoprotein 3, Hiep Vu, Byungjoon Kwon, Kyoung-Jin Yoon, William W. Laegreid, Asit K. Pattnaik, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

Passive administration of porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing antibodies (NAbs) can effectively protect pigs against PRRSV infection. However, after PRRSV infection, pigs typically develop a weak and deferred NAb response. One major reason for such a meager NAb response is the phenomenon of glycan shielding involving GP5, a major glycoprotein carrying one major neutralizing epitope. We describe here a type II PRRSV field isolate (PRRSV-01) that is highly susceptible to neutralization and induces an atypically rapid, robust NAb response in vivo. Sequence analysis shows that PRRSV-01 lacks two N-glycosylation sites, normally present in wild-type (wt) PRRSV strains, …

Analysis And Interpretation Of Factors Leading To Increased Aids Prevalence In Sub-Saharan Africa, William Kalhorn

Department of Environmental Studies: Undergraduate Student Theses

My thesis research project focuses on the major factors that are contributing to the worst disease epidemic on the planet today. I have aimed to determine what may be some of the most important factors contributing to highly variable difference in HIV/AIDS prevalence rates are among the regions of East, West, and South Africa.  The HIV/AIDS epidemic has been growing and expanding to new areas of the world since the first case arose in 1959 in what is now called Kinshasa, Congo (Avert, 2011). The AIDS/HIV epidemic is believed to have originated on the African continent, and this is where …

Archaeoparasitology Of Chaco Canyon, Rachel Paseka

Department of Environmental Studies: Undergraduate Student Theses

Ancient cultures of the Colorado Plateau have been a focus of archaeoparasitology since its inception, and a vast parasitological history is recorded in coprolites preserved in this arid region. The inhabitants of Chaco Canyon dominated Ancestral Puebloan culture between 1050 and 1120 AD and were responsible for the construction of great towns, road systems, and early agriculture. Analysis of the parasites preserved in fecal remains contributes to an increased knowledge of ancient Chacoan health and culture. Nineteen coprolites from four sites in Chaco Canyon were rehydrated and analyzed microscopically for parasite remains. Rhabditiform and filariform nematode larvae were found from …

Ontology For Psychophysiological Dysregulation Of Anger/Aggression, Swathi Vasanthapuram

School of Computing: Dissertations, Theses, and Student Research

The advancement of Information Technology in the last four decades led to the use of computers in medicine. A new area called Medical Informatics has emerged. This area comprises the application of IT to healthcare with the aim of creating tools that help healthcare personnel diagnose and treat patients more accurately and efficiently. IT not only provides tools for storing, integrating, and updating patient information base but also for processing information efficiently. One of such tools is a Clinical Decision Support System. Ontologies are an integral part of clinical decision support systems because they help formalize and integrate domain knowledge. …

Spatio-Temporal Analysis Of Malaria In Paraguay, Nicole M. Wayant

Department of Geography: Dissertations, Theses, and Student Research

Malaria is a mosquito-borne disease that has afflicted humans for thousands of years. Today it is considered a re-emerging disease. Malaria is most prevalent in tropical and subtropical parts of the world. The disease has been linked to several environmental parameters, including precipitation, temperature, and deforestation. However, these relationships have mainly been studied in Africa and have not been explored in other parts of the world. The study area for this thesis was the South American country of Paraguay.

Paraguay has experienced an oscillation in malaria cases over the past 20 years, with monthly cases ranging from 0 to 1,200. …

A Mark–Recapture Technique For Monitoring Feral Swine Populations, Matthew M. Reidy, Tyler A. Campbell, David G. Hewitt

United States Department of Agriculture Wildlife Services: Staff Publications

Techniques to monitor populations of feral swine (Sus scrofa) relative to damage control activities are needed on rangelands. Our objectives were to describe and assess a mark–recapture technique using tetracycline hydrochloride (TH) for monitoring feral swine populations. We established bait stations at study sites in southern and central Texas. During 1 d, we replaced normal soured corn bait with bait containing TH and counted the number of feral swine that consumed bait with observers. We conducted feral swine removal using box-style traps and helicopters, at which time we collected teeth for TH analysis. In southern Texas, we estimated …

Replacement Alternatives For Beef Cow Herds: An Analysis Of Retaining Non-Pregnant Cows, Trenton T. Bohling

Department of Agricultural Economics: Dissertations, Theses, and Student Research

A non-pregnant cow is a liability to a producer. Over the last four years, cow-calf producers have had an increased number of non-pregnant cows due to factors like environmental conditions and diseases like trichomoniasis. While most research has indicated that culling a non-pregnant female and replacing the cow with retained heifers, purchased heifers, or purchased cows are the only economic alternatives, recent trends in the cattle market have suggested that keeping a non-pregnant cow may also be an alternative.

Annual beef cow budgets were created based on typical Nebraska Sandhills conditions. Revenues and costs in these budgets vary according to …

Host And Distribution Lists Of Chiggers (Trombiculidae And Leeuwenhoekiidae), Of North American Wild Vertebrates North Of Mexico, Brianne L. Walters, John O. Whitaker Jr., Nicholas S. Gikas, William J. Wrenn

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Information concerning chiggers found on wild vertebrates of North America north of Mexico is summarized. Included are lists a) of the chiggers organized taxonomically, b) hosts from which each species has been reported, and c) states and provinces with references for each separate record

Adherence Inhibition Of Cronobacter Sakazakii And Other Pathogens By Prebiotic Oligosaccharides, Plant Extracts, And Other Naturally Derived Molecules, Maria I. Quintero

Department of Food Science and Technology: Dissertations, Theses, and Student Research

For most bacterial pathogens, adherence of the bacterium to the surface of the host cell tissue is a necessary first step for colonization and infection.  Agents that inhibit adherence, therefore, could be useful for preventing infections.  The goal of this research was to assess the anti-adherent activity of several food-grade prebiotic carbohydrates, plant extracts, and other naturally-derived molecules against enteric pathogens. First, the antiadherent effect of galactooligosaccharides (GOS), polydextrose (PDX), and a GOS-PDX blend was tested against two strains of Cronobacter “Enterobacter” sakazakii. When measured microscopically or by cultural methods, significant reductions in adherence (56% and 71%, respectively) of C. …

Molecular Structures Of Prrsv That Contribute To Prrsv Protective Immunity, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

One of the objectives of the proposal is to delineate the regions of the two glycoproteins (GP2 and GP4) of PRRSV that interact with CD163. Other objectives of the proposal are to generate antibodies to these small regions of the glycoproteins as well as to the full-length proteins for future studies to determine if any of these antibodies possess PRRSV neutralizing activity.

To carry out the studies in the proposed objectives, we generated a series of mutants of GP2 as well as GP4 proteins in which various regions were specifically removed by manipulating the plasmids encoding these proteins. We then …

La Crosse Fish Health Center Onalaska, Wisconsin

United States Fish and Wildlife Service: Publications

The La Crosse Fish Health Center (Center) is located in Onalaska, Wisconsin in the southwest corner of the state bordering the Mississippi River. The Center is one of nine fish health laboratories operated by the U.S. Fish and Wildlife Service to serve the National Fish Hatchery System. The Center was established in 1962 and moved to its current facilities in 1995. The staff consists of eight permanent w/ 1 vacancy, 4 experience program students, and 2 student volunteers.

As an aquatic animal health laboratory, the Center services include health inspections, diagnostics, training opportunities, and technical information exchange to five Regional …

Genomic And Resistance Gene Homolog Diversity Of The Dominant Tallgrass Prairie Species Across The U.S. Great Plains Precipitation Gradient, Matthew N. Rouse, Amgad A. Saleh, Amadou Seck, Kathleen H. Keeler, Steven E. Travers, Scot H. Hulbert, Karen A. Garrett

School of Biological Sciences: Faculty Publications

Background: Environmental variables such as moisture availability are often important in determining species prevalence and intraspecific diversity. The population genetic structure of dominant plant species in response to a cline of these variables has rarely been addressed. We evaluated the spatial genetic structure and diversity of Andropogon gerardii populations across the U.S. Great Plains precipitation gradient, ranging from approximately 48 cm/year to 105 cm/year.

Methodology/Principal Findings: Genomic diversity was evaluated with AFLP markers and diversity of a disease resistance gene homolog was evaluated by PCR-amplification and digestion with restriction enzymes. We determined the degree of spatial genetic structure …

Nefdc Exchnage, Volume 22, Number 2, Spring 2011, New England Faculty Development Consortium

New England Faculty Development Consortium Exchange

Contents

President's message: Who is your mentor? - Tom Thibodeau, New England Institute of Technology

Sparks, insights, and affirmations: reflections of a mentor - Denise Marchionda, Middlesex Community College

Engaging students through social media to promote learning - Suzanne Markham, Mount Ida College

Vampires, and zombies, and ghosts, oh my… run! The undead in the college classroom - Kristine Larsen, Central Connecticut State University

Service learning as a way to engage students and serve the community - William Stargard, Pine Manor College

Save the date! NEFDC 2011 Fall Conference, Friday, November 11, 2011, College of the Holy Cross

Models for …

Human Papillomavirus Genotype Concordance Within Zambian Couples, Kgomotso Makhaola

School of Biological Sciences: Dissertations, Theses, and Student Research

Human Papillomavirus (HPV) is strongly associated with the development of cervical cancer, and has also been identified in other anogenital cancers such as penile, oral, and anal cancers. In regions like Sub Saharan Africa where the prevalence of HIV/AIDS is high,   increased rates of HPV infections have also been observed, however the effect of HIV/AIDS on the transmission of HPV is not yet well understood.  In this study specimens for HIV, HPV testing and pathology were collected from male participants and their female partners at a Urology department in Lusaka University Teaching Hospital, Zambia. Seventy four samples were collected but …

Protection Against Divergent Influenza H1n1 Virus By A Centralized Influenza Hemagglutinin, Eric A. Weaver, Adam M. Rubrum, Richard J. Webby, Michael A. Barry

School of Biological Sciences: Faculty Publications

Influenza poses a persistent worldwide threat to the human population. As evidenced by the 2009 H1N1 pandemic, current vaccine technologies are unable to respond rapidly to this constantly diverging pathogen. We tested the utility of adenovirus (Ad) vaccines expressing centralized consensus influenza antigens. Ad vaccines were produced within 2 months and protected against influenza in mice within 3 days of vaccination. Ad vaccines were able to protect at doses as low as 107 virus particles/kg indicating that approximately 1,000 human doses could be rapidly generated from standard Ad preparations. To generate broadly cross-reactive immune responses, centralized consensus antigens were constructed …

Advances And Future Challenges In Adenoviral Vector Pharmacology And Targeting, Reeti Khare, Christopher Y. Chen, Eric A. Weaver, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Adenovirus is a robust vector for therapeutic applications, but its use is limited by our understanding of its complex in vivo pharmacology. In this review we describe the necessity of identifying its natural, widespread, and multifaceted interactions with the host since this information will be crucial for efficiently redirecting virus into target cells. In the rational design of vectors, the notion of overcoming a sequence of viral “sinks” must be combined with re-targeting to target populations with capsid as well as shielding the vectors from pre-existing or toxic immune responses. It must also be noted that most known adenoviral pharmacology …

Design Of A Protective Single-Dose Intranasal Nanoparticle-Based Vaccine Platform For Respiratory Infectious Diseases, Bret D. Ulery, Devender Kumar, Amanda E. Ramer-Tait, Dennis W. Metzger, Michael J. Wannemeuhler, Balaji Narasimhan

Department of Food Science and Technology: Faculty Publications

Despite the successes provided by vaccination, many challenges still exist with respect to controlling new and re-emerging infectious diseases. Innovative vaccine platforms composed of adaptable adjuvants able to appropriately modulate immune responses, induce long-lived immunity in a single dose, and deliver immunogens in a safe and stable manner via multiple routes of administration are needed. This work describes the development of a novel biodegradable polyanhydride nanoparticle-based vaccine platform administered as a single intranasal dose that induced long-lived protective immunity against respiratory disease caused by Yesinia pestis, the causative agent of pneumonic plague. Relative to the responses induced by the …

Isolation By Distance Explains Genetic Structure Of Buggy Creek Virus, A Bird-Associated Arbovirus, Abinash Padhi, Amy T. Moore, Mary Bomberger Brown, Jerome E. Foster, Martin Pfeffer, Charles R. Brown

School of Natural Resources: Faculty Publications

Many of the arthropod-borne viruses (arboviruses) show extensive genetic variability and are widely distributed over large geographic areas. Understanding how virus genetic structure varies in space may yield insight into how these pathogens are adapted to and dispersed by different hosts or vectors, the relative importance of mutation, drift, or selection in generating genetic variability, and where and when epidemics or epizootics are most likely to occur. However, because most arboviruses tend to be sampled opportunistically and often cannot be isolated in large numbers at a given locale, surprisingly little is known about their spatial genetic structure on the local …

Assessment Of Pathways For The Introduction And Spread Of Mycobacterium Bovis In The United States, Katie Portacci, Jason Lombard, Lauren Abrahamsen, Eric Bush, Charles Fossler, Robert Harris, Kamina Johnson, Ryan S. Miller, Dianna Mitchell, Randy Pritchard, Steven Sweeney, Todd Weaver

Zoonotics and Wildlife Disease: Publications

Bovine tuberculosis (TB) was responsible for more losses among U.S. farm animals in the early 20th century than all other infectious diseases combined. The Cooperative State-Federal Tuberculosis Eradication Program (established in 1917 and administered by APHIS, State animal health agencies, and U.S. livestock producers) has nearly eradicated bovine TB from the nation’s livestock population. However, despite the many accomplishments of the program, bovine TB remains a serious and costly disease of livestock in the United States. In 1992, VS conducted an assessment to identify pathways for the introduction and spread of bovine TB, in order to develop the most effective …

Invasive Plant Species As Potential Bioenergy Producers And Carbon Contributors, Stephen L. Young, Gayathri Gopalakrishnan, Deepak R. Keshwani

West Central Research and Extension Center, North Platte

Among recent advances in developing cellulosic and noncellulosic biofuel sources, corn, switchgrass, and others (for example, camelina and canola) have risen to the top. In surveying the landscape, a potentially promising new area of bioenergy production exists—invasive plant species. The addition of invasive plant species as a bioenergy source will help to diversify the nation’s energy dependence and help in the reduction of the negative environmental and social impacts from energy crop production. In addition, belowground carbon stores may provide an opportunity to reduce the impacts associated with global climate change.

In the agriculture sector, harvesting equipment is well advanced …

Factors Affecting Space Use Overlap By White-Tailed Deer In An Urban Landscape, W. David Walter, Jeff Beringer, Lonnie P. Hansen, Justin W. Fischer, Joshua J. Millspaugh, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Variation in the size and overlap of space use by white-tailed deer (Odocoileus virginianus) has broad implications for managing deer–human conflicts and disease spread and transmission in urban landscapes. Understanding which factors affect overlap of home range by various segments (i.e., age, sex) of an urban deer population has implications to direct contact between deer on disease epidemiology. We assessed size of home range and overlap of space use using the volume of intersection index (VI) for deer in an urban landscape by sex, age, season, and time of day. We found mean space use was larger for …

Persistent Infection Or Successive Reinfection Of Deer Mice With Bartonella Vinsonii Subsp. Arupensis, Ying Bai, Charles H. Calisher, Michael Y. Kosoy, J. Jeffrey Root, Jeffrey B. Doty

United States Department of Agriculture Wildlife Services: Staff Publications

Bartonella infections are common in rodents. From 1994 to 2006, longitudinal studies of a rodent community, consisting mainly of deer mice (Peromyscus maniculatus), were conducted in southwestern Colorado to study hantaviruses. Blood samples from deer mice captured one or more times during the period 2003 to 2006 (n = 737) were selected to study bartonellae in deer mice. Bartonellae were found to be widely distributed in that population, with an overall prevalence of 82.4% (607/737 mice). No correlation was found between bartonella prevalence and deer mouse weight or sex. Persistent or successive infections with bartonellae were observed …

Low Levels Of Hydrogen Peroxide Stimulate Corneal Epithelial Cell Adhesion, Migration, And Wound Healing, Qing Pan, Wen-Ya Qiu, Ya-Nan Huo, Yu-Feng Yao, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

PURPOSE. Intracellular reactive oxygen species have been reported to associate with growth factor and integrin signalings in promoting cell adhesion in many cell types. This study is to explore if exogenous H2O2 at low levels can be beneficial to cell adhesion, migration, and wound healing.

METHODS. Primary rabbit corneal epithelial cells treated with 0–70 M H2O2 were tested for viability by MTT assay, adhesion by centrifugation assay, focal contacts of vinculin and F-actin by immunofluorescence, activated Src(pY416), EGF receptor (pY845), vinculin(pY1065), FAK(pY397), and FAK(pY576) by immunoblotting. Cell migration was examined with 0–50 M H2O2 using the scratch wound technique. Corneal …

Host-Microbial Symbiosis In The Vertebrate Gastrointestinal Tract And The Lactobacillus Reuteri Paradigm, Jens Walter, Robert A. Britton, Stefan Roos

Department of Food Science and Technology: Faculty Publications

Vertebrates engage in symbiotic associations with vast and complex microbial communities that colonize their gastrointestinal tracts. Recent advances have provided mechanistic insight into the important contributions of the gut microbiome to vertebrate biology, but questions remain about the evolutionary processes that have shaped symbiotic interactions in the gut and the consequences that arise for both the microbes and the host. Here we discuss the biological principles that underlie microbial symbiosis in the vertebrate gut and the potential of the development of mutualism. We then review phylogenetic and experimental studies on the vertebrate symbiont Lactobacillus reuteri that have provided novel insight …

Real-Time Dynamic Imaging Of Virus Distribution In Vivo, Sean E. Hofherr, Kristen E. Adams, Christopher Y. Chen, Shannon May, Eric A. Weaver, Michael A. Barry

School of Biological Sciences: Faculty Publications

The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to ascertain tissue localization. They also obviate the ability to perform longitudinal or kinetic studies in one animal. To track viruses after systemic infection, we have labeled adenoviruses with a near-infrared (NIR) fluorophore and imaged these after intravenous injection in mice. Imaging was able to track and quantitate virus particles entering the jugular vein simultaneous with …

Is Wildlife Going To The Dogs? Impacts Of Feral And Free-Roaming Dogs On Wildlife Populations, Julie K. Young, Kirk A. Olson, Richard P. Reading, Sukh Amgalanbaatar, Joel Berger

United States Department of Agriculture Wildlife Services: Staff Publications

In human-populated landscapes, dogs (Canis familiaris) are often the most abundant terrestrial carnivore. However, dogs can significantly disrupt or modify intact ecosystems well beyond the areas occupied by people. Few studies have directly quantified the environmental or economic effects of free-roaming and feral dogs. Here, we review wildlife-dog interactions and provide a case study that focuses on interactions documented from our research in Mongolia to underscore the need for studies designed to best determine how dogs affect native wildlife and especially imperiled populations. We suggest additional research, public awareness campaigns, and the exclusion of dogs from critical wildlife …

Integrative Approaches To Disease Control: The Value Of International Collaborations, Thomas J. Deliberto, Dale L. Nolte, William (Bill) H. Clay

United States Department of Agriculture Wildlife Services: Staff Publications

One health and biodiversity issues are global and complex. As such, they require international collaboration if we hope to achieve the goal of minimizing disease impacts on humans and animals, while maintaining biodiversity and ecosystem integrity. This will require motivated teams to overcome barriers to collaboration, by focusing on the proven keys to success and developing networks of biological, physical and social scientists as well as decision-makers and stakeholders. These collaborations will require facilitation by governments and international organizations.

Finally, we must move towards a more holistic, transdisciplinary approach to international collaborations. New forms of knowledge, institutional structure and problem-solving …

Caring For Compromised Cattle: Assessing Animals At Risk

Nebraska Beef Quality Assurance Program

Cattlemen have long recognized the need to properly care for their livestock. Sound animal husbandry practices – based on research and decades of practical experience – are known to impact the well-being of cattle, individual animal health and herd productivity. There is no doubt that producers work hard to ensure that their cattle are properly cared for throughout all stages of production. Unfortunately, it’s a fact of raising cattle that some animals will become injured to the extent that they are unable to stand or walk without assistance.

Office Of Research And Economic Development Annual Report 2010-2011

Office of Research and Economic Development: Publications

Collaborations, Partnerships Drive Innovation 1

Discovery Could Spark Smaller, Faster Electronics 2

MRSEC Fosters Collaboration 3

Harnessing Laser Power Creates Precise Nanostructures 5

Nanohybrids Promise ‘Best of Both Worlds’ 6

Water for Food Institute Building Partnerships 8

World Water Expert to Lead Institute 9

Understanding Aquifer Recharge 10

Targeted Research Investments Hedge Against Food Crisis 11

Uncovering New Perspectives on Whitman 12

Civil War Washington Going Digital 14

Humanities Grants Support Language, Digital Initiatives 15

Improving Children’s Reading Comprehension 16

Transforming Early Childhood Education 17

Bullying: Filling Gaps Between Research, Practice 18

Preparing Military Kids for Success in School 19 …

Infectious Diseases In Yellowstone’S Canid Community, Emily S. Almberg, Paul C. Cross, L. David Mech, Doug W. Smith, Jennifer W. Sheldon, Robert L. Crabtree

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Each summer Yellowstone Wolf Project staff visit den sites to monitor the success of wolf reproduction and pup rearing behavior. For the purposes of wolf monitoring, Yellowstone National Park (YNP) is divided into two study areas, the northern range and the interior, each distinguished by their ecological and physiographical differences. The 1,000 square kilometer northern range, characterized by lower elevations (1,500–2,200 m), serves as prime winter habitat for ungulates and supports a higher density of wolves than the interior (20–99 wolves/1,000 km² versus 2–11 wolves/1,000 km²). The interior of the park encompasses 7,991 square kilometers, is higher …