Digital Commons Network^™

Using Network Sampling And Recruitment Data To Understand Social Structures Related To Community Health In A Population Of People Who Inject Drugs In Rural Puerto Rico, Mayra Coronado-García, Courtney R. Thrash, Melissa L. Welch-Lazoritz, G. Robin Gauthier, Juan Carlos Reyes, Bilal Khan, Kirk Dombrowski

Department of Sociology: Faculty Publications

Objective: This research examined the social network and recruitment patterns of a sample of people who inject drugs (PWIDs) in rural Puerto Rico, in an attempt to uncover systematic clustering and between-group social boundaries that potentially influence disease spread.

Methods: Respondent driven sampling was utilized to obtain a sample of PWID in rural Puerto Rico. Through eight initial “seeds”, 317 injection drug users were recruited. Using recruitment patterns of this sample, estimates of homophily and affiliation were calculated using RDSAT.

Results: Analyses showed clustering within the social network of PWID in rural Puerto Rico. In particular, females showed a very …

Implementing A Metapopulation Bass Diffusion Model Using The R Package Desolve, Jim Duggan

The R Journal

Diffusion is a fundamental process in physical, biological, social and economic settings. Consumer products often go viral, with sales driven by the word of mouth effect, as their adoption spreads through a population. The classic diffusion model used for product adoption is the Bass diffusion model, and this divides a population into two groups of people: potential adopters who are likely to adopt a product, and adopters who have purchased the product, and influence others to adopt. The Bass diffusion model is normally captured in an aggregate form, where no significant consumer differences are modeled. This paper extends the Bass …

Seroprevalence Of Antibodies Against Kaposi's Sarcoma-Associated Herpesvirus Among Hiv-Negative People In China, Tiejun Zhang, Zhenqiu Liu, Jun Wang, Veenu Minhas, Charles Wood, Gary M. Clifford, Na He, Silvia Franceschi

Nebraska Center for Virology: Faculty Publications

Background: Little information on the prevalence of Kaposi’s sarcoma associated herpesvirus (KSHV) among HIV-negative individuals is available from Asia.

Methods: In the present study, we report findings from a new survey of KSHV in 983 HIV-negative male migrants from Shanghai and their combination with previous similar surveys of 600 female migrants, 600 female sex-workers (FSW), 1336 sexually transmitted infection (STI) clinic male patients, 439 intravenous drug-users (IVDU), and 226 men having sex with men (MSM) from China. KSHV-specific antibodies against latent and lytic antigens were assessed using Sf9 and BC3 monoclonal immunofluorescence assay. Age-adjusted prevalence ratios (PR) and 95% confidence …

Peanut Allergen Threshold Study (Pats): Novel Single-Dose Oral Food Challenge Study To Validate Eliciting Doses In Children With Peanut Allergy, Jonathan O'B. Hourihane, Katrina J. Allen, Wayne G. Shreffler, Gillian Dunngalvin, Julie A. Nordlee, Giovanni A. Zurzolo, Audrey Dunngalvin, Lyle C. Gurrin, Joseph L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Background: Eliciting doses (EDs) of allergenic foods can be defined by the distribution of threshold doses for subjects within a specific population. The ED₀₅ is the dose that elicits a reaction in 5% of allergic subjects. The predicted ED₀₅ for peanut is 1.5 mg of peanut protein (6 mg of whole peanut).

Objective: We sought to validate the predicted peanut ED₀₅ (1.5 mg) with a novel single-dose challenge. Methods: Consecutive eligible children with peanut allergy in 3 centers were prospectively invited to participate, irrespective of previous reaction severity. Predetermined criteria for objective reactions were used …

Effects Of Egg Yolk On Piglet Growth Performance, Circulating Immunoglobulins, Biomarkers Of Intestinal Integrity, And Microbial Community, Kelly C. Moore

Department of Animal Science: Dissertations, Theses, and Student Research

A total of 72 crossbred pigs weaned at 24 days were assigned to a completely randomized design (CRD) arrangement of treatments to determine the effects of egg yolk on a nursery piglet’s growth performance, circulating immunoglobulins, biomarkers of intestinal integrity, and microbial community. Pigs were provided with spray-dried plasma (SDP), spray-dried egg yolk (SDEY), or a control diet. During Phase III, ADG was affected by treatment (P < 0.10). Pigs consuming the egg yolk diet had greater (P < 0.05) ADG vs. the plasma group for Phase III. Circulating immunoglobulins (IgA and IgG) were not affected by dietary treatment. C-reactive protein (CRP) and haptoglobin (Hp) concentrations remained constant during the experiment. Treatment did effect Glugacon like peptide-2 (GLP-2) concentration (P < 0.01) where pigs fed egg yolk had the greatest concentration. Microbial OTUs were similar among treatments (P > 0.8), but there were changes over time; from d 0 to Phase I and II there was an increase in diversity (P < 0.01) followed by a decrease in …

Lysine Residues Of Interferon Regulatory Factor 7 Affect The Replication And Transcription Activatormediated Lytic Replication Of Kaposi’S Sarcomaassociated Herpesvirus/Human Herpesvirus 8, Tianzheng Zhang, Ying Wang, Li Zhang, Bin Liu, Jinhui Xie, Charles Wood, Jinzhong Wang

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) infection goes through latent and lytic phases, which are controlled by the viral replication and transcription activator (RTA). Upon KSHV infection, the host responds by suppressing RTA-activated lytic gene expression through interferon regulatory factor 7 (IRF-7), a key regulator of host innate immune response. Lysine residues are potential sites for post-translational modification of IRF-7, and were suggested to be critical for its activity. In this study, we analysed the 15 lysine residues for their effects on IRF-7 function by site-directed mutagenesis. We found that some mutations affect the ability of IRF-7 to activate interferon (IFN)-a1 and …

Chitosan Nanoparticle Modifications For Improved Gene Delivery In An Oral Dna Vaccine Application, Austin Helmink

Honors Program: Senior Projects (Public)

Vaccines represent one of the most significant medical innovations of the 20^th century, resulting in the eradication or near eradication of a handful of deadly diseases. However, many infectious diseases remain resistant to effective vaccination, largely due to a lack full immune activation by traditional protein-based vaccines. A promising alternative vaccination strategy is the emerging development of DNA vaccines, which rely upon the delivery of exogenous genetic material to host cells encoding for a viral or bacterial antigen in order to induce a robust immune response by closely mimicking live infection. The delivery of genetic material requires a carrier …

Inventory Of Wildlife Use Of Mortality Pits As Feeding Sites: Implications Of Pathogen Exposure, Jeremy W. Ellis, Susan A. Shriner, Hailey E. Mclean, Lauren Petersen, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

To better understand the use of mortality pits by wildlife and possible pathogen dissemination from the resulting wildlife contact in these areas, we used 8 camera traps on 4 mortality pits in Colorado from June to December 2014 to create a species inventory and establish use estimates for those species. We observed 43 species visiting (in or near) the mortality pits during 1,168 total camera trap days. Of these, 24 species directly interacted with the mortality pits or carcasses contained within them. The most common visitors to mortality pits were raccoons (Procyon lotor), coyotes (Canis latrans), …

Th1 Differentiation Drives The Accumulation Of Intravascular, Non-Protective Cd4 T Cells During Tuberculosis, Michelle A. Sallin, Shunsuke Sakai, Keith D. Kauffman, Howard A. Young, Jinfang Zhu, Daniel L. Barber

United States Public Health Resources

Recent data indicate that the differentiation state of Th1 cells determines their protective capacity against tuberculosis. Therefore, we examined the role of Th1-polarizing factors in the generation of protective and non-protective subsets of Mtb-specific Th1 cells. We find that IL-12/23p40 promotes Th1 cell expansion and maturation beyond the CD73+CXCR3+T-betdim stage, and T-bet prevents deviation of Th1 cells into Th17 cells. Nevertheless, IL- 12/23p40 and T-bet are also essential for the production of a prominent subset of intravascular CX3CR1+KLRG1+ Th1 cells that persists poorly and can neither migrate into the lung parenchyma nor control Mtb growth. Furthermore, T-bet suppresses development of …

Coccolithoviruses: A Review Of Cross-Kingdom Genomic Thievery And Metabolic Thuggery, Jozef I. Nissimov, António Pagarete, Fangrui Ma, Sean Cody, David D. Dunigan, Susan A. Kimmance, Michael J. Allen

Nebraska Center for Virology: Faculty Publications

Coccolithoviruses (Phycodnaviridae) infect and lyse the most ubiquitous and successful coccolithophorid in modern oceans, Emiliania huxleyi. So far, the genomes of 13 of these giant lytic viruses (i.e., Emiliania huxleyi viruses—EhVs) have been sequenced, assembled, and annotated. Here, we performed an in-depth comparison of their genomes to try and contextualize the ecological and evolutionary traits of these viruses. The genomes of these EhVs have from 444 to 548 coding sequences (CDSs). Presence/absence analysis of CDSs identified putative genes with particular ecological significance, namely sialidase, phosphate permease, and sphingolipid biosynthesis. The viruses clustered into distinct clades, based on their DNA polymerase …

Foxo3 Increases Mir-34a To Cause Palmitate-Induced Cholangiocyte Lipoapoptosis, Sathish Kumar Natarajan, Bailey A. Stringham, Ashley M. Mohr, Cody J. Wehrkamp, Sizhao Lu, Mary Anne Phillippi, Duygu Dee Harrison-Findik, Justin L. Mott

School of Veterinary and Biomedical Sciences: Faculty Publications

Nonalcoholic steatohepatitis (NASH) patients have elevated plasma saturated free fatty acid levels. These toxic fatty acids can induce liver cell death and our recent results demonstrated that the biliary epithelium may be susceptible to lipotoxicity. Here, we explored the molecular mechanisms of cholangiocyte lipoapoptosis in cell culture and in an animal model of NASH. Treatment of cholangiocytes with palmitate (PA) showed increased caspase 3/7 activity and increased levels of cleaved poly (ADP-ribose) polymerase and cleaved caspase 3, demonstrating cholangiocyte lipoapoptosis. Interestingly, treatment with PA significantly increased the levels of microRNA miR-34a, a pro-apoptotic microRNA known to be elevated in NASH. …

Reoccurrence Of Avian Influenza A(H5n2) Virus Clade 2.3.4.4 In Wild Birds, Alaska, Usa, 2016, Dong-Hun Lee, Mia K. Torchetti, Mary Lea Killian, Thomas J. Deliberto, David E. Swayne

United States Department of Agriculture Wildlife Services: Staff Publications

We report reoccurrence of highly pathogenic avian influenza A(H5N2) virus clade 2.3.4.4 in a wild mallard in Alaska, USA, in August 2016. Identification of this virus in a migratory species confirms low-frequency persistence in North America and the potential for re-dissemination of the virus during the 2016 fall migration.

Habitat Selection By The Northern Long-Eared Myotis (Myotis Septentrionalis) In The Midwestern United States: Life In A Shredded Farmscape, Jeremy A. White, Patricia Freeman, Cliff A. Lemen

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Populations of the Northern Long-Eared Myotis (Myotis septentrionalis) have declined dramatically in recent years in eastern North America due to white-nose syndrome. Although M. septentrionalis was once common in parts of eastern North America, few studies have examined habitat selection of this species in an agricultural landscape. We used acoustical methods to quantify bat activity and construct a habitat model of M. septentrionalis in an intensively farmed area in the Midwestern United States, where mortality from white-nose syndrome has not yet been observed. Our study confirms that M. septentrionalis prefers forest and avoids open habitats in this agricultural …

Reframing Communication About Zika And Mosquitoes To Increase Disease Prevention Behavior, Amanda E. Sorensen, Rebecca C. Jordan, Shannon Ladeau

School of Natural Resources: Faculty Publications

Emergence of invasive vector species and the diseases they carry present a clear danger to the public as well as a challenge for scientists and experts to control effectively. Given the urgent need to address this phenomenon, we suggest that desired public action toward these invasive vectors can be motivated through intentional framing in science communication. In this paper, we sought to evaluate the effect of framing about ZIKV (Zika virus) on reported willingness to comply with mosquito prevention action. Post framing intervention, we found a significant increase (N = 26, p < 0.001) in individuals willing to take preventative action against mosquitoes. By methodically investigating best communication practices, this study and others can help practitioners mobilize communities to address large-scale ecological problems. Additionally, the principles outlined here may be transferrable to other communication efforts about ecological issues outside of ZIKV and mosquitoes.

A Quantitative Framework For Assessing Ecological Resilience, Didier L. Baho, Craig R. Allen, Ahjond Garmestani, Hannah Fried-Petersen, Sophia E. Renes, Lance Gunderson, David G. Angeler

School of Natural Resources: Faculty Publications

Quantitative approaches to measure and assess resilience are needed to bridge gaps between science, policy, and management. In this paper, we suggest a quantitative framework for assessing ecological resilience. Ecological resilience as an emergent ecosystem phenomenon can be decomposed into complementary attributes (scales, adaptive capacity, thresholds, and alternative regimes) that embrace the complexity inherent to ecosystems. Quantifying these attributes simultaneously provides opportunities to move from the assessment of specific resilience within an ecosystem toward a broader measurement of its general resilience. We provide a framework that is based on reiterative testing and recalibration of hypotheses that assess complementary attributes of …

Hexapod Herald - Vol. 29, No. 1, January 2017

Hexapod Herald and Other Entomology Department Newsletters

Content: Welcome - Faculty News - Grants - Congratulations - Student News - Blast from the Past - Publications - Condolences (Dr. Kenneth P. Pruess)

Research And Creative Activity, July 1, 2016-June 30, 2017: Major Sponsored Programs And Faculty Awards For Research And Creative Activity, University Of Nebraska-Lincoln, Office Of Research And Economic Development, University Of Nebraska-Lincoln

Office of Research and Economic Development: Publications

Introduction by Steve Goddard, Interim Vice Chancellor for Research and Economic Development, University of Nebraska-Lincoln:

This booklet highlights successes in research, scholarship and creative activity of the University of Nebraska–Lincoln faculty during the fiscal year July 1, 2016-June 30, 2017.

It lists investigators, project titles and funding sources on major grants and sponsored program awards received during the year; fellowships and other recognitions and honors bestowed on our faculty; books published by faculty; performances, exhibitions and other creative activity by our faculty; and intellectual property licenses and patents issued for the products of Nebraska research.

This booklet is an impressive …

Modeling And Statistical Issues Related To Salmonella In Low Water Activity Foods, Sofia M. Santillana Farakos, Michelle Danyluk, Donald Schaffner, Régis Pouillot, Linda J. Harris, Bradley P. Marks

United States Food and Drug Administration: Publications

The presence and survival of Salmonella in low water activity (a_w) foods continues to pose a challenge for the food industry. Peer‐reviewed literature data on prevalence and levels of contamination of Salmonella in low water activity foods in the United States are limited. Available published data include those on: Salmonella contamination on nuts and peanuts (Calhoun et al., 2013), almonds (Danyluk et al., 2007; Bansal et al., 2010), pecans (Brar, Strawn, and Danyluk, 2016), and walnuts (Davidson et al., 2015); prevalence and levels of Salmonella on spices (Van Doren et al., 2013); as …

Using Decision Analysis To Support Proactive Management Of Emerging Infectious Wildlife Diseases, Evan H. Campbell Grant, Erin Muths, Rachel A. Katz, Stefano Canessa, Michael J. Adams, Jennifer R. Ballard, Lee Berger, Cheryl J. Briggs, Jeremy Th Coleman, Matthew J. Gray, M Camille Harris, Reid N. Harris, Blake Hossack, Kathryn P. Huyvaert, Jonathan Kolby, Karen R. Lips, Robert E. Lovich, Hamish I. Mccallum, Joseph R. Mendelson Iii, Priya Nanjappa, Deanna H. Olson, Jenny G. Powers, Katherine Ld Richgels, Robin E. Russell, Benedikt R. Schmidt, Annemarieke Spitzen-Van Der Sluijs, Mary Kay Watry, Douglas C. Woodhams, C Leann White

United States Navy: Publications

Despite calls for improved responses to emerging infectious diseases in wildlife, management is seldom considered until a disease has been detected in affected populations. Reactive approaches may limit the potential for control and increase total response costs. An alternative, proactive management framework can identify immediate actions that reduce future impacts even before a disease is detected, and plan subsequent actions that are conditional on disease emergence. We identify four main obstacles to developing proactive management strategies for the newly discovered salamander pathogen Batrachochytrium salamandrivorans (Bsal). Given that uncertainty is a hallmark of wildlife disease management and that associated decisions are …

Microbial Ecology Of Fresh Vegetables, J. Zheng, A. De Jesus, S. Sahu, A. E. Hayford, Y. Luo, A. R. Datta, E. W. Brown, R. Bell

United States Food and Drug Administration: Publications

The total consumption of fresh and processed vegetables has exceeded 123 kg per capita in the United States and 118 kg per capita in the European Union (http://www.helgilibrary.com/ indicators/index/vegetable‐consumption‐percapita) in 2009. Changes in lifestyles and consumption trends have prompted the sustained growth of fresh‐cut or minimally processed vegetables that are fresh and ready‐to‐eat (RTE). In 2011, for example, total US fresh‐cut produce sales through both food service and retail channels were estimated to surpass $27 billion (Cook et al., 2012).

2017 Annual Campus Security And Fire Safety Report, University Of Nebraska-Lincoln, University Police, University Of Nebraska-Lincoln

University of Nebraska-Lincoln, University Police

2017 Annual Campus Security and Fire Safety Report, University of Nebraska-Lincoln

Safety and security information for the University of Nebraska-Lincoln, including crime and fire statistics for the 2016 calendar year, and the information required by the Drug-Free Schools and Communities Act of 1989. All data are submitted to the United States Department of Education according to law.

Varicella Seroepidemiology In United States Air Force Recruits: A Retrospective Cohort Study Comparing Immunogenicity Of Varicella Vaccination And Natural Infection, Joshua R. Duncan, Catherine T. Witkop, Bryant J. Webber, Amy A. Costello

Uniformed Services University of the Health Sciences

Background/Objectives: Infection with varicella zoster virus (VZV) produces lifelong immunity, but duration of post-vaccination immunity has not been established. The purpose of this study is to determine if a difference exists in the long-term seropositivity of anti-VZV antibodies in a cohort of young adults who were vaccinated against varicella as compared to a similar cohort with a history of chickenpox disease, and to determine which variables best predict waning seropositivity following varicella vaccination.

Methods: This retrospective cohort study captures immunization and serology data from approximately 10,000 recruits who entered basic military training between January 1, 2008, and December 31, 2015, …

Hypothesis: Exosomal Micrornas As Potential Biomarkers For Schizophrenia, Vijaya Raghavan, Manish Bhomia, Isabel Torres, Sanjeev Jain, Kevin K. Wang

Uniformed Services University of the Health Sciences

Schizophrenia is a serious mental disorder with lifelong morbidity and increased mortality. Currently, the diagnosis of the disorder is based on patient history and clinical examination, but it has a low inter-rater reliability and validity. Various biological variables, such as event related potentials, hormonal levels, brain ventricular volume and hippocampal size, have been put forth as objective markers to diagnose schizophrenia, but none with the desired sensitivity and specificity. It has been shown that microRNAs play a vital role in gene regulation in schizophrenia and have been proposed as possible biomarkers for the disease. When compared to the free microRNAs …

National And State-Specific Shingles Vaccination Among Adults Aged >60 Years, Peng-Jun Lu, Alissa O'Halloran, Walter W. Williams, Rafael Harpaz

United States Public Health Resources

Introduction: Shingles (herpes zoster) causes substantial morbidity, especially among older adults. The shingles vaccine has been recommended for people aged >60 years since 2006. This study assessed recent shingles vaccination at national and state levels among adults aged >60 years.

Methods: The 2014 Behavioral Risk Factor Surveillance System data were analyzed in 2015 to assess shingles vaccination coverage among adults aged >60 years at national and state levels. Multivariable logistic regression and predictive marginal models identified factors independently associated with vaccination.

Results: Shingles vaccination coverage among adults aged >60 years was 31.8% (95% CI=31.4%, 32.2%). Among states, shingles vaccination coverage …

Coupling Ecological And Social Network Models To Assess “Transmission” And “Contagion” Of An Aquatic Invasive Species, Danielle Haak, Brian D. Fath, Valery E. Forbes, Dustin R. Martin, Kevin L. Pope

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Network analysis is used to address diverse ecological, social, economic, and epidemiological questions, but few efforts have been made to combine these field-specific analyses into interdisciplinary approaches that effectively address how complex systems are interdependent and connected to one another. Identifying and understanding these cross-boundary connections improves natural resource management and promotes proactive, rather than reactive, decisions. This research had two main objectives; first, adapt the framework and approach of infectious disease network modeling so that it may be applied to the socio-ecological problem of spreading aquatic invasive species, and second, use this new coupled model to simulate the spread …

Host Allometry Influences The Evolution Of Parasite Host-Generalism: Theory And Meta-Analysis, Josephine G. Walker, Amy Hurford, Jo Cable, Amy R. Ellison, Stephen J. Price, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Parasites vary widely in the diversity of hosts they infect: some parasite species are specialists—infecting just a single host species, while others are generalists, capable of infecting many. Understanding the factors that drive parasite host-generalism is of basic biological interest, but also directly relevant to predicting disease emergence in new host species, identifying parasites that are likely to have unidentified additional hosts, and assessing transmission risk. Here, we use mathematical models to investigate how variation in host body size and environmental temperature affect the evolution of parasite host-generalism. We predict that parasites are more likely to evolve a generalist strategy …

Host Allometry Influences The Evolution Of Parasite Host-Generalism: Theory And Meta-Analysis, Josephine G. Walker, Amy Hurford, Jo Cable, Amy R. Ellison, Stephen J. Price, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Parasites vary widely in the diversity of hosts they infect: some parasite species are specialists—infecting just a single host species, while others are generalists, capable of infecting many. Understanding the factors that drive parasite host-generalism is of basic biological interest, but also directly relevant to predicting disease emergence in new host species, identifying parasites that are likely to have unidentified additional hosts, and assessing transmission risk. Here, we use mathematical models to investigate how variation in host body size and environmental temperature affect the evolution of parasite host-generalism. We predict that parasites are more likely to evolve a generalist strategy …

When Children Are Water: Representation Of Central American Migrant Children In Public Discourse And Implications For Educators, Theresa Catalano

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Since June, 2014 when the U.S. government began to document an increase in unaccompanied/separated children arriving in the United States from Central America, these children have become a frequent topic in media discourse. Because rhetoric about immigration issues have been shown to affect schooling of these children, the present paper aims to examine how these children are represented in the discourse of one community. Findings from this critical multimodal discourse analysis reveal multiple strategies of representation that result in the dominant metaphor of IMMIGRANT CHILDREN ARE DANGEROUS WATER and negative perceptions that have implications for the education of these students.

Interdisciplinary Teaching Of Theatre And Human Rights In Honors, Maria Szasz

Honors in Practice Online Archive

One of the centerpieces of honors education is careful research and thorough analysis of what we teach and why we teach our chosen subjects. In creating my honors class Theatre and Human Rights, I explored how I would teach the course and the various components best suited to teaching this topic. After first considering the topic of human rights and its relevance to theatre in an honors context, I then considered the value of interdisciplinary teaching in such a course and what its impact could be on helping students understand human rights, specifically through the study of Athol Fugard’s 1982 …

Balancing Interests And Risk Of Error: What Quarantine Process Is Due After Ebolamania, Jennifer Jolly-Ryan

Nebraska Law Review

I. Introduction

II. The Good Samaritan Nurse’s Return Home from Sierra Leone

III. Historical Background of Quarantines

IV. State and Federal Quarantine Powers ... A. The States’ Broad Police Powers and Quarantines … B. The Federal Government’s Commerce Power and Quarantines

V. Procedural Due Process Limits on Quarantine Powers … A. Weighing Individual Liberty Interests Quarantines Infringe ... B. Weighing the Risk of Error in Quarantine Procedures ... C. Weighing Government Interests, Adding Eradication of Discrimination

VI. Conclusion