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Concentrations Of Purine Metabolites Are Elevated In Human Fluids From Adults And Infants And In Livers From Mice Fed Diets Depleted Of Bovine Milk Exosomes And Their Rna Cargos, Ana Aguilar Lozano Nov 2018

Concentrations Of Purine Metabolites Are Elevated In Human Fluids From Adults And Infants And In Livers From Mice Fed Diets Depleted Of Bovine Milk Exosomes And Their Rna Cargos, Ana Aguilar Lozano

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Since miRNAs discovery, evidence keeps accumulating on their role in human physiology for homeostasis and implications during disease development. Upon the discovery that miRNAs could be encapsulated in exosomes and provide protection, evidence keeps accumulating on the possibility for miRNAs to be transferred between distant cells and elicit gene expression changes, and clinical trials are being developed to identify miRNAs in body fluids that could predict disease outcomes based on miRNA encapsulation in exosomes making them stable in body fluids. Recently, studies have shown that dietary miRNAs encapsulated in exosomes can be absorbed in mice and humans, and on the …


Development & Analysis Of A Science Outreach Event For Elementary Aged Children: Archie's Late-Night Party, Bailey Peterson Mar 2018

Development & Analysis Of A Science Outreach Event For Elementary Aged Children: Archie's Late-Night Party, Bailey Peterson

Honors Program: Senior Projects (Public)

Science Outreach is important because it facilitates learning and an increase in interest in the sciences. This thesis outlines the development of a set of stations as part of “Archie’s Late-Night Party,” an event held in June, 2017 at Morrill Hall. The goal of the Morrill Hall event was to create multiple activities and educational stations for elementary aged children in various scientific fields. Under the guidance of Dr. Gwen Bachman, I created two stations to present at the event: “Sleep & Hibernation” and “Nocturnal Vision.” I describe the multiple factors and challenges that go into building stations that are …


Preparing For The Future: 2017–2018 Annual Report Jan 2018

Preparing For The Future: 2017–2018 Annual Report

University of Nebraska's National Strategic Research Institute: Publications

3 Letter from the Executive Director Robert Hinson

4 About NSRI-- Combatting WMD. Focusing on Deterrence.

6 Key Accomplishments/ Highlights of Business Operations Research Focus Areas

8 Nuclear Detection and Forensics

10 Active and Passive Defense Against Weapons of Mass Destruction

12 Detection of Chemical and Biological Weapons

14 Consequence Management

16 Mission-Related Research

18 Education & Training

20 Student & Faculty Engagement

22 NSRI/NU Key Journal Articles, 2017–2018

23 NSRI Board of Directors


Establishment Of A Human Ipsc- And Nanofiber-Based Microphysiological Blood–Brain Barrier System, Dianjun Qi, Shaohua Wu, Haishuang Lin, Mitchell A. Kuss, Yuguo Lei, Alexey Krasnoslobodtsev, Shaheen Ahmed, Chi Zhang, Hyung Joon Kim, Peng Jiang, Bin Duan Jan 2018

Establishment Of A Human Ipsc- And Nanofiber-Based Microphysiological Blood–Brain Barrier System, Dianjun Qi, Shaohua Wu, Haishuang Lin, Mitchell A. Kuss, Yuguo Lei, Alexey Krasnoslobodtsev, Shaheen Ahmed, Chi Zhang, Hyung Joon Kim, Peng Jiang, Bin Duan

Department of Chemical and Biomolecular Engineering: Faculty Publications

The blood–brain barrier (BBB) is an active and complex diffusion barrier that separates the circulating blood from the brain and extracellular fluid, regulates nutrient transportation, and provides protection against various toxic compounds and pathogens. Creating an in vitro microphysiological BBB system, particularly with relevant human cell types, will significantly facilitate the research of neuropharmaceutical drug delivery, screening, and transport, as well as improve our understanding of pathologies that are due to BBB damage. Currently, most of the in vitro BBB models are generated by culturing rodent astrocytes and endothelial cells, using commercially available transwell membranes. Those membranes are made of …


Milk Exosomes Are Bioavailable And Distinct Microrna Cargos Have Unique Tissue Distribution Patterns, Sonia Manca, Bijaya Upadhyaya, Ezra Mutai, Amy T. Desaulniers, Rebecca A. Cederberg, Brett R. White, Janos Zempleni Jan 2018

Milk Exosomes Are Bioavailable And Distinct Microrna Cargos Have Unique Tissue Distribution Patterns, Sonia Manca, Bijaya Upadhyaya, Ezra Mutai, Amy T. Desaulniers, Rebecca A. Cederberg, Brett R. White, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Exosomes participate in cell-to-cell communication, facilitated by the transfer of RNAs, proteins and lipids from donor to recipient cells. Exosomes and their RNA cargos do not exclusively originate from endogenous synthesis but may also be obtained from dietary sources such as the inter-species transfer of exosomes and RNAs in bovine milk to humans. Here, we assessed the bioavailability and distribution of exosomes and their microRNA cargos from bovine, porcine and murine milk within and across species boundaries. Milk exosomes labeled with fluorophores or fluorescent fusion proteins accumulated in liver, spleen and brain following suckling, oral gavage and intravenous administration in …


Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel Nov 2017

Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel

Department of Biochemistry: Faculty Publications

Rationale: Cardiac lipotoxicity, characterized by increased uptake, oxidation and accumulation of lipid intermediates, contributes to cardiac dysfunction in obesity and diabetes. However, mechanisms linking lipid overload and mitochondrial dysfunction are incompletely understood.

Objective: To elucidate the mechanisms for mitochondrial adaptations to lipid overload in postnatal hearts in vivo.

Methods and Results: Using a transgenic mouse model of cardiac lipotoxicity overexpressing long-chain acyl-CoA synthetase 1 in cardiomyocytes, we show that modestly increased myocardial fatty acid uptake leads to mitochondrial structural remodeling with significant reduction in minimum diameter. This is associated with increased palmitoyl-carnitine oxidation and increased reactive oxygen species (ROS) generation …


Metabolic Regulation By Stress Mediators In Fetal Ovine Skeletal Muscle And Adult Bovine Myoblasts, Elena Marie Merrick Aug 2017

Metabolic Regulation By Stress Mediators In Fetal Ovine Skeletal Muscle And Adult Bovine Myoblasts, Elena Marie Merrick

Department of Animal Science: Dissertations, Theses, and Student Research

Our objective was to determine the effect of stress mediators such as inflammatory cytokines and β adrenergic agonists on developing skeletal muscle. Fetal sheep exposed to chronic maternal inflammation had decreased skeletal muscle glucose oxidation rates when stimulated with insulin or TNFα. In addition, activation of Akt by insulin was decreased after maternal inflammation when compared to controls and there were not differences in skeletal muscle fiber type ratios between the two treatment groups. This indicates that maternal inflammation does not affect overall oxidative capacity in the developing fetus, but rather insulin signaling pathways are disrupted.

We sought to determine …


Answers To Common Misconceptions About Biological Evolution, Leah M. Abebe, Blake Bartels, Kaitlyn M. Caron, Adam M. Gleeson, Samuel N. Johnson, Tyler J. Kluza, Nicholas W. Knopik, Kristen N. Kramer, Masiel S. Maza, Kaitlyn A. Stava, Kaitlyn Sullivan, Jordan T. Trimble, Robert M. Zink May 2017

Answers To Common Misconceptions About Biological Evolution, Leah M. Abebe, Blake Bartels, Kaitlyn M. Caron, Adam M. Gleeson, Samuel N. Johnson, Tyler J. Kluza, Nicholas W. Knopik, Kristen N. Kramer, Masiel S. Maza, Kaitlyn A. Stava, Kaitlyn Sullivan, Jordan T. Trimble, Robert M. Zink

Papers in Evolution

“When you have an established scientific emergent truth, it is true whether or not you believe in it.” Neil deGrasse Tyson, Science in America. Very few people outside of a particular scientific discipline can actually say they understand it, because most do not have the training to “speak the language.” They are then not particularly bothered by its tenets and predictions. Of all the major branches of science, however, evolutionary biology is an exception to this generalization because even though people are not versed in the field, they sometimes have a negative, knee-jerk objection. This objection is often because they …


Lactose Intolerance: An Overview Of The Facts And Their Implications, Noelle M. Yeo Mar 2017

Lactose Intolerance: An Overview Of The Facts And Their Implications, Noelle M. Yeo

Honors Program: Senior Projects (Public)

Lactose intolerance is often blamed for the symptoms such as bloating, diarrhea, gas, abdominal pain, and nausea, that ail many people. Patients often do not seek proper diagnosis from a physician and create their own treatment plans, severely restricting lactose intake, without professional guidance. Even those who do seek the care of a physician find that diagnosis is complicated by less-than ideal testing and confusion due to the symptoms common to many other conditions. The misconceptions and inability to confirm a diagnosis of lactose intolerance can cause nutrient deficiencies in these patients, as well as begin a pattern of unnecessary …


Vmed 646: Animal Physiology Ii—A Peer Review Of Teaching Project Benchmark Portfolio, Renee M. Mcfee Jan 2017

Vmed 646: Animal Physiology Ii—A Peer Review Of Teaching Project Benchmark Portfolio, Renee M. Mcfee

UNL Faculty Course Portfolios

This Peer Review of Teaching Project portfolio focuses on the Animal Physiology II course which is required for first year veterinary medicine students. Weekly quizzes assess baseline knowledge and had been administered individually and in groups. I hypothesized the discontinuation of group quizzes would increase student effort when preparing for quizzes. Unit exams involve scenario-based questions and require students to apply information. I hypothesized the implementation of group exams would help under-performing students improve their ability to apply information they had learned. Exams were still taken individually prior to being taken in groups to encourage adequate preparation. Student impacts were …


Stable Engraftment Of Bifidobacterium Longum Ah1206 In The Human Gut Depends On Individualized Features Of The Resident Microbiome, María X. Maldonado-Gómez, Inés Martínez, Francesca Bottacini, Amy O’Callaghan, Marco Ventura, Douwe Van Sinderen, Benjamin Hillmann, Pajau Vangay, Dan Knights, Robert W. Hutkins, Jens Walter Oct 2016

Stable Engraftment Of Bifidobacterium Longum Ah1206 In The Human Gut Depends On Individualized Features Of The Resident Microbiome, María X. Maldonado-Gómez, Inés Martínez, Francesca Bottacini, Amy O’Callaghan, Marco Ventura, Douwe Van Sinderen, Benjamin Hillmann, Pajau Vangay, Dan Knights, Robert W. Hutkins, Jens Walter

Department of Food Science and Technology: Faculty Publications

Live bacteria (such as probiotics) have long been used to modulate gut microbiota and human physiology, but their colonization is mostly transient. Conceptual understanding of the ecological principles as they apply to exogenously introduced microbes in gut ecosystems is lacking. We find that, when orally administered to humans, Bifidobacterium longum AH1206 stably persists in the gut of 30% of individuals for at least 6 months without causing gastrointestinal symptoms or impacting the composition of the resident gut microbiota. AH1206 engraftment was associated with low abundance of resident B. longum and underrepresentation of specific carbohydrate utilization genes in the pre-treatment microbiome. …


Fungi In The Healthy Human Gastrointestinal Tract, Heather E. Hallen-Adams, Mallory J. Suhr Oct 2016

Fungi In The Healthy Human Gastrointestinal Tract, Heather E. Hallen-Adams, Mallory J. Suhr

Department of Food Science and Technology: Faculty Publications

Many species of fungi have been detected in the healthy human gut; however, nearly half of all taxa reported have only been found in one sample or one study. Fungi capable of growing in and colonizing the gut are limited to a small number of species, mostly Candida yeasts and yeasts in the family Dipodascaceae (Galactomyces, Geotrichum, Saprochaete). Malassezia and the filamentous fungus Cladosporium are potential colonizers; more work is needed to clarify their role. Other commonly-detected fungi come from the diet or environment but either cannot or do not colonize (Penicillium and Debaryomyces species, which are …


Physiology And Political Beliefs: A Response To Knoll, O’Daniel, And Cusato, Johnathan C. Peterson, Kevin Smith, John Hibbing Sep 2016

Physiology And Political Beliefs: A Response To Knoll, O’Daniel, And Cusato, Johnathan C. Peterson, Kevin Smith, John Hibbing

Department of Political Science: Faculty Publications

n a recent paper in this journal, Knoll et al. question three studies from our laboratory. In this response to that paper, we address deficiencies in their “reproduction.” Notably, we demonstrate that their data provide little evidence of a negativity bias among research subjects, suggesting a failure not only to reproduce findings from our earlier studies, but also a failure to find a widely acknowledged universal human physiological response trait. This situation raises a number of questions regarding the data on which their analyses are based. We explore these questions below and speculate that Knoll et al.’s data collection procedures …


Viruses, Vaccines And The Public, Judy Diamond, Julia Mcquillan, Amy Spiegel, Patricia Wonch Hill, Rebecca Smith, John T. West, Charles Wood May 2016

Viruses, Vaccines And The Public, Judy Diamond, Julia Mcquillan, Amy Spiegel, Patricia Wonch Hill, Rebecca Smith, John T. West, Charles Wood

World of Viruses

Current research in virology is changing public conceptions about vaccines and infectious disease. The University of Nebraska State Museum collaborated with research virologists, science writers, artists and learning researchers to create public outreach materials about viruses and infectious disease. The project, funded by the National Institute of Health’s SEPA program, developed comics, a book with Carl Zimmer, and other materials and programs. The project launched three kinds of learning research: 1) a survey of Nebraska adults on their opinions about vaccines and infectious disease; 2) a study comparing the mental models of viruses, vaccines and infection from virologists, teachers, and …


A Mycobacterium Avium Subsp. Paratuberculosis Predicted Serine Protease Is Associated With Acid Stress And Intraphagosomal Survival, Abirami Kugadas, Elise A. Lamont, John Bannantine, Fernanda Miyagaki Shoyama, Evan Brenner, Harish K. Janagama, Srinand Sreevatsan Jan 2016

A Mycobacterium Avium Subsp. Paratuberculosis Predicted Serine Protease Is Associated With Acid Stress And Intraphagosomal Survival, Abirami Kugadas, Elise A. Lamont, John Bannantine, Fernanda Miyagaki Shoyama, Evan Brenner, Harish K. Janagama, Srinand Sreevatsan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The ability to maintain intra-cellular pH is crucial for bacteria and other microbes to survive in diverse environments, particularly those that undergo fluctuations in pH. Mechanisms of acid resistance remain poorly understood in mycobacteria. Although, studies investigating acid stress in M. tuberculosis are gaining traction, few center on Mycobacterium avium subsp. paratuberculosis (MAP), the etiological agent of chronic enteritis in ruminants. We identified a MAP acid stress response network involved in macrophage infection. The central node of this network was MAP0403, a predicted serine protease that shared an 86% amino acid identity with MarP in M. tuberculosis. Previous studies confirmed …


Student Buy-In To Active Learning In A College Science Course, Andrew J. Cavanagh, Oriana R. Aragon, Xinnian Chen, Brian A. Couch, Mary F. Durham, Aiyana Bobrownicki, David I. Hanauer, Mark J. Graham Jan 2016

Student Buy-In To Active Learning In A College Science Course, Andrew J. Cavanagh, Oriana R. Aragon, Xinnian Chen, Brian A. Couch, Mary F. Durham, Aiyana Bobrownicki, David I. Hanauer, Mark J. Graham

School of Biological Sciences: Faculty Publications

The benefits of introducing active learning in college science courses are well established, yet more needs to be understood about student buy-in to active learning and how that process of buy-in might relate to student outcomes. We test the exposure–persuasion– identification–commitment (EPIC) process model of buy-in, here applied to student (n = 245) engagement in an undergraduate science course featuring active learning. Student buy-in to active learning was positively associated with engagement in self-regulated learning and students’ course performance. The positive associations among buy-in, self-regulated learning, and course performance suggest buy-in as a potentially important factor leading to student …


Urolithin A, C And D, But Not Iso-Urolithin A And Urolithin B, Attenuate Triglyceride Accumulation In Human Cultures Of Adipocytes And Hepatocytes, Inhae Kang, Yongeun Kim, Francisco A. Tomás-Barberán, Juan Carlos Espín, Soonkyu Chung Jan 2016

Urolithin A, C And D, But Not Iso-Urolithin A And Urolithin B, Attenuate Triglyceride Accumulation In Human Cultures Of Adipocytes And Hepatocytes, Inhae Kang, Yongeun Kim, Francisco A. Tomás-Barberán, Juan Carlos Espín, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Scope: Urolithins (Uro) are ellagic acid (EA)-derived metabolites produced by gut microbes. There is a growing interest in the biological activities of Uro. Our aim was to evaluate the impacts of Uro on regulating triglyceride (TG) accumulation using cultures of primary human adipocytes and hepatoma Huh7 cells.

Methods and Results: UroA, B, C, D, and iso-UroA, were used to determine the effect of Uro on adipogenesis and lipogenesis. Individual Uro (30 M) were added to human adipogenic stem cells (hASCs) during differentiation. UroA, C and D, but not iso-UroA and UroB, significantly inhibited new fat cell formation by decreasing TG …


Efficacy Of Galactooliosaccharide (Gos) And/Or Rhamnose-Based Synbiotics In Enhancing Ecological Performance Of Lactobacillus Reuteri In The Human Gut And Characterization Of Its Gos Metabolic System, Monchaya Rattanaprasert Dec 2015

Efficacy Of Galactooliosaccharide (Gos) And/Or Rhamnose-Based Synbiotics In Enhancing Ecological Performance Of Lactobacillus Reuteri In The Human Gut And Characterization Of Its Gos Metabolic System, Monchaya Rattanaprasert

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Probiotic L. reuteri has potential to produce antimicrobial compounds and secrete immunosuppressive factors. These metabolic attributes could benefit the human host by providing colonization resistance (competitive and metabolic exclusion) against enteropathogens and mitigating inflammation. As metabolically active cells are fundamental to such probiotic properties, synbiotic approaches that supply L. reuteri with a source(s) of carbon, energy, and/or external electron acceptor for cell growth in the gut environment could therefore prompt the probiotic to engage in beneficial activities. In this study, the efficacy of GOS and/or rhamnose-based synbiotic approaches in promoting colonization persistence and metabolic activity of L. reuteri was evaluated. …


Characterization Of Inhibitors Of Fatty Acid Transport Protein-2 In Cell And Animal Models, Nipun Saini Oct 2015

Characterization Of Inhibitors Of Fatty Acid Transport Protein-2 In Cell And Animal Models, Nipun Saini

Department of Biochemistry: Dissertations, Theses, and Student Research

Obesity is correlated with insulin resistance and elevated levels of glucose, triglycerides and free fatty acids in blood. This affects overall metabolism and leads to disease. In the obese state, fat also accumulates in non-adipose tissue including liver, muscle and pancreas, where it can lead to cellular dysfunction and death. Currently, only a limited number of drugs are available to combat obesity and it is clear that new drugs, which more narrowly target the metabolic pathways involved, are required. Fatty Acid Transport Proteins (FATPs) are bifunctional proteins involved in the uptake and activation of fatty acids by esterification with coenzyme …


Influence Of Native And Processed Cereal Grain Fibers On Gut Health, Junyi Yang Jul 2015

Influence Of Native And Processed Cereal Grain Fibers On Gut Health, Junyi Yang

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Cereal fibers that can be metabolized by gut microbiota have been shown to promote the growth of beneficial bacteria in the gut. Increased consumption of cereal fibers may improve host / gut microbiota interactions in obesity and other metabolic diseases by normalizing gut dysbiosis. The present dissertation describes four research projects to assess the impact of cereal dietary fibers on gut microbiota and host metabolism. In the first study, we determined the treatment temperatures for production of soluble, non-digestible, feruloylated oligo- and polysaccharides (FOPS) from maize bran and wheat bran, and determined the fermentation properties of partially purified FOPS from …


Effects Of Ibuprofen And Caffeine Concentrations On The Common Bed Bug (Cimex Lectularius L.) Feeding And Fecundity, Ralph B. Narain, Shripat T. Kamble May 2015

Effects Of Ibuprofen And Caffeine Concentrations On The Common Bed Bug (Cimex Lectularius L.) Feeding And Fecundity, Ralph B. Narain, Shripat T. Kamble

Department of Entomology: Faculty Publications

Bed bugs are ectoparasites of humans and require a blood meal for their growth and reproduction. Since humans consume Ibuprofen as pain medication and drink coffee (caffeine), bed bugs are likely to acquire these drugs through blood feeding. In this study, we determined the biological effects of Ibuprofen and caffeine on bed bug feeding, fecundity and egg hatch. Five concentrations of Ibuprofen and caffeine were incorporated into reconstituted human blood (RHB). Control treatment had no Ibuprofen or caffeine. Each treatment had six replications. Groups of 20 adult bed bugs (10 males: 10 females)/treatment/replication were pre-weighed, allowed to feed for 45 …


Effect Of The Physical Environment On Teacher Satisfaction With Indoor Environmental Quality In Early Learning Schools, Stuart Shell May 2015

Effect Of The Physical Environment On Teacher Satisfaction With Indoor Environmental Quality In Early Learning Schools, Stuart Shell

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

While the quantity and quality of teacher-child interactions plays a key role in emotional and cognitive development for children, there is scant evidence regarding the contribution of physical environment to child outcomes. This study seeks to understand better the relative importance of variables within the physical environment for occupants. The research design targets teachers’ satisfaction with the physical environment as the outcome variable, based on the assumption that teachers who are more satisfied with their classroom provide higher-quality interactions with children. Teachers from two early learning schools with a total of 31 classrooms completed a written survey that asked about …


Development Of A Fatal Noncompressible Truncal Hemorrhage Model With Combined Hepatic And Portal Venous Injury In Normothermic Normovolemic Swine, Ujwal R. Yanala, Jason M. Johanning, Iraklis I. Pipinos, Gustavo F. Larsen, William H. Velander, Mark A. Carlson Sep 2014

Development Of A Fatal Noncompressible Truncal Hemorrhage Model With Combined Hepatic And Portal Venous Injury In Normothermic Normovolemic Swine, Ujwal R. Yanala, Jason M. Johanning, Iraklis I. Pipinos, Gustavo F. Larsen, William H. Velander, Mark A. Carlson

William H. Velander Publications

Noncompressible truncal hemorrhage and brain injury currently account for most early mortality of warfighters on the battlefield. There is no effective treatment for noncompressible truncal hemorrhage, other than rapid evacuation to a surgical facility. The availability of an effective field treatment for noncompressible truncal hemorrhage could increase the number of warfighters salvaged from this frequently-lethal scenario. Our intent was to develop a porcine model of noncompressible truncal hemorrhage with a ,50% one-hour mortality so that we could develop new treatments for this difficult problem. Normovolemic normothermic domestic swine (barrows, 3 months old, 34–36 kg) underwent one of three injury types …


Program And Proceedings: Nebraska Academy Of Sciences 1880–2014, 134th Anniversary Year, One Hundred-Twenty-Fourth Annual Meeting, April 11, 2014 Apr 2014

Program And Proceedings: Nebraska Academy Of Sciences 1880–2014, 134th Anniversary Year, One Hundred-Twenty-Fourth Annual Meeting, April 11, 2014

Nebraska Academy of Sciences: Programs and Proceedings

Program

Aeronautics and Space Science

Collegiate Academy: Biology

Chemistry and Physics

Collegiate Academy: Chemistry and Physics

Applied Science and Technology

Biological and Medical Sciences

Aeronautics and Space Science, Poster Session

Anthropology

Maiben Memorial Lecture: "Education, Careers and Opportunities in Engineering and Metallurgy in Nebraska," Terry Rasmussen, Nucor Steel, Norfolk, Nebraska

Teaching of Science and Mathematics

Anthropology

Earth Science


Passive Biaxial Mechanical Properties And In Vivo Axial Pre-Stretch Of The Diseased Human Femoropopliteal And Tibial Arteries, Alexey Kamenskiy, Iraklis I. Pipinos, Yuris A. Dzenis, Clay Lomneth, Syed A. Jaffar Kazmi, Nicholas Y. Phillips, Jason N. Mactaggart Mar 2014

Passive Biaxial Mechanical Properties And In Vivo Axial Pre-Stretch Of The Diseased Human Femoropopliteal And Tibial Arteries, Alexey Kamenskiy, Iraklis I. Pipinos, Yuris A. Dzenis, Clay Lomneth, Syed A. Jaffar Kazmi, Nicholas Y. Phillips, Jason N. Mactaggart

Department of Mechanical and Materials Engineering: Faculty Publications

Surgical and interventional therapies for atherosclerotic lesions of the infrainguinal arteries are notorious for high rates of failure. Frequently, this leads to expensive reinterventions, return of disabling symptoms, or limb loss. Interaction between the artery and repair material likely plays an important role in reconstruction failure, but data describing the mechanical properties and functional characteristics of human femoropopliteal and tibial arteries are currently not available. Diseased superficial femoral (SFA, n=10), popliteal (PA, n=8), and tibial arteries (TA, n=3) from 10 patients with critical limb ischemia were tested to determine passive mechanical properties using planar biaxial extension. All …


Development Of A Fatal Noncompressible Truncal Hemorrhage Model With Combined Hepatic And Portal Venous Injury In Normothermic Normovolemic Swine, Ujwal R. Yanala, Jason M. Johanning, Iraklis I. Pipinos, Gustavo F. Larsen, William H. Velander, Mark A. Carlson Jan 2014

Development Of A Fatal Noncompressible Truncal Hemorrhage Model With Combined Hepatic And Portal Venous Injury In Normothermic Normovolemic Swine, Ujwal R. Yanala, Jason M. Johanning, Iraklis I. Pipinos, Gustavo F. Larsen, William H. Velander, Mark A. Carlson

Department of Chemical and Biomolecular Engineering: Faculty Publications

Noncompressible truncal hemorrhage and brain injury currently account for most early mortality of warfighters on the battlefield. There is no effective treatment for noncompressible truncal hemorrhage, other than rapid evacuation to a surgical facility. The availability of an effective field treatment for noncompressible truncal hemorrhage could increase the number of warfighters salvaged from this frequently-lethal scenario. Our intent was to develop a porcine model of noncompressible truncal hemorrhage with a ,50% one-hour mortality so that we could develop new treatments for this difficult problem. Normovolemic normothermic domestic swine (barrows, 3 months old, 34–36 kg) underwent one of three injury types …


Misunderstanding The Female Athlete Triad: Refuting The Ioc Consensus Statement On Relative Energy Deficiency In Sport (Red-S), Mary Jane De Souza, Nancy I. Williams, Aurelia Nattiv, Elizabeth Joy, Madhusmita Misra, Anne B. Loucks, Gordon Matheson, Marion P. Olmsted, Michelle Barrack, Rebecca J. Mallinson, Jenna C. Gibbs, Marci Goolsby, Jeanne F. Nichols, Barbara Drinkwater, Charlotte (Barney) Sanborn, Rosemary Agostini, Carol L. Otis, Mimi D. Johnson, Anne Zeni Hoch, Julia M. K. Alleyne, L. Tyler Wadsworth, Karsten Koehler, Jaci Vanheest, Paula Harvey, Amanda K. Weiss Kelly, Michael Fredericson, George A. Brooks, Emma O'Donnell, Lisa R. Callahan, Margot Putukian, Lauren Costello, Suzanne Hecht, Mitchell J. Rauh, Jacalyn Mccomb Jan 2014

Misunderstanding The Female Athlete Triad: Refuting The Ioc Consensus Statement On Relative Energy Deficiency In Sport (Red-S), Mary Jane De Souza, Nancy I. Williams, Aurelia Nattiv, Elizabeth Joy, Madhusmita Misra, Anne B. Loucks, Gordon Matheson, Marion P. Olmsted, Michelle Barrack, Rebecca J. Mallinson, Jenna C. Gibbs, Marci Goolsby, Jeanne F. Nichols, Barbara Drinkwater, Charlotte (Barney) Sanborn, Rosemary Agostini, Carol L. Otis, Mimi D. Johnson, Anne Zeni Hoch, Julia M. K. Alleyne, L. Tyler Wadsworth, Karsten Koehler, Jaci Vanheest, Paula Harvey, Amanda K. Weiss Kelly, Michael Fredericson, George A. Brooks, Emma O'Donnell, Lisa R. Callahan, Margot Putukian, Lauren Costello, Suzanne Hecht, Mitchell J. Rauh, Jacalyn Mccomb

Department of Nutrition and Health Sciences: Faculty Publications

We are concerned that readers of the IOC paper will be confused and misled by the poorly referenced statements and frank (and sometimes dangerous) errors in the paper. The IOC authors should publish a correction of these and other errors noted. Broadening research of low energy availability in other groups, such as the male athlete, athletes of diverse ethnicities and the disabled athlete may help to advance science and may one day warrant introduction of a specific term for whatever serious clinical sequelae of energy deficiency may be discovered in future research on men.

Research on the “Female Athlete Triad” …


Screening Of Mycobacterium Avium Subsp. Paratuberculosis Mutants For Attenuation In A Bovine Monocyte-Derived Macrophage Model, Elise A. Lamont, Adel M. Talaat, Paul M. Coussens, John Bannantine, Yrjo T. Grohn, Robab Katani, Ling-Ling Li, Vivek Kapur, Srinand Sreevatsan Jan 2014

Screening Of Mycobacterium Avium Subsp. Paratuberculosis Mutants For Attenuation In A Bovine Monocyte-Derived Macrophage Model, Elise A. Lamont, Adel M. Talaat, Paul M. Coussens, John Bannantine, Yrjo T. Grohn, Robab Katani, Ling-Ling Li, Vivek Kapur, Srinand Sreevatsan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Vaccination remains a major tool for prevention and progression of Johne’s disease, a chronic enteritis of ruminants worldwide. Currently there is only one licensed vaccine within the United States and two vaccines licensed internationally against Johne’s disease. All licensed vaccines reduce fecal shedding of Mycobacterium avium subsp. paratuberculosis (MAP) and delay disease progression. However, there are no available vaccines that prevent disease onset. A joint effort by the Johne’s Disease Integrated Program (JDIP), a USDA-funded consortium, and USDA—APHIS/VS sought to identify transposon insertion mutant strains as vaccine candidates in part of a three phase study. The focus of the Phase …


Holobiont Nutrition: Considering The Role Of The Gastrointestinal Microbiota In The Health Benefits Of Whole Grains, Jens Walter, Ines Martinez, Devin J. Rose Apr 2013

Holobiont Nutrition: Considering The Role Of The Gastrointestinal Microbiota In The Health Benefits Of Whole Grains, Jens Walter, Ines Martinez, Devin J. Rose

Food for Health: Publications

Intake of whole grains and other food products high in dietary fiber have long been linked to the prevention of chronic diseases associated with inflammation. A contribution of the gastrointestinal microbiota to these effects has been suggested, but little is known on how whole grains interact with gut bacteria. We have recently published the first human trial that made use of next-generation sequencing to determine the effect of whole grains (whole grain barley, brown rice or a mixture of the two) on fecal microbiota structure and tested for associations between the gut microbiota and blood markers of inflammation, glucose and …


Agricultural Research Magazine, April 2013 Apr 2013

Agricultural Research Magazine, April 2013

Agricultural Research Magazine

Table of Contents

4 Irrigation Wastewater: Waste Not, Want Not

6 Two Approaches for Optimizing Water Productivity

8 Compounds in Whole-Grain Rice Varieties

10 New Discovery Makes Detecting Johne’s Disease Easier

12 House Fly Virus Stops Flies from Reproducing

14 Improved Vitamin B12 Test May Help Young and Old Alike

16 Rooting Out a Novel Stress Syndrome in Pigs

18 A Modeling Milestone for Soil Phosphorus Management

20 New Technologies for Studying Crops and Crop Diseases

23 Locations Featured in This Magazine Issue