Digital Commons Network^™

Intrauterine Transfer Of Polyunsaturated Fatty Acids In Mother–Infant Dyads As Analyzed At Time Of Delivery, Vanessa Woodard, Melissa Thoene, Matthew Van Ormer, Maranda Thompson, Corrine Hanson, Sathish Kumar Natarajan, Maheswari Mukherjee, Ana Yuil-Valdes, Tara M. Nordgren, Arzu Ulu, Kristina Harris Jackson, Ann Anderson-Berry

Department of Nutrition and Health Sciences: Faculty Publications

Polyunsaturated fatty acids (PUFAs) are essential for fetal development, and intrauterine transfer is the only supply of PUFAs to the fetus. The prevailing theory of gestational nutrient transfer is that certain nutrients (including PUFAs) may have prioritized transport across the placenta. Numerous studies have identified correlations between maternal and infant fatty acid concentrations; however, little is known about what role maternal PUFA status may play in differential intrauterine nutrient transfer. Twenty mother–infant dyads were enrolled at delivery for collection of maternal and umbilical cord blood, and placental tissue samples. Plasma concentrations of PUFAs were assessed using gas chromatography (GC‐FID). Intrauterine …

Highlighting Membrane Protein Structure And Function: A Celebration Of The Protein Data Bank, Fei Li, Pascal F. Egea, Alex J. Vecchio, Ignacio Asial, Meghna Gupta, Joana Paulino, Ruchika Bajaj, Miles Sasha Dickinson, Shelagh Fergunson-Miller, Brian C. Monk, Robert M. Stroud

Department of Biochemistry: Faculty Publications

Biological membranes define the boundaries of cells and compartmentalize the chemical and physical processes required for life. Many biological processes are carried out by proteins embedded in or associated with such membranes. Determination of membrane protein (MP) structures at atomic or near-atomic resolution plays a vital role in elucidating their structural and functional impact in biology. This endeavor has determined 1198 unique MP structures as of early 2021. The value of these structures is expanded greatly by deposition of their three-dimensional (3D) coordinates into the Protein Data Bank (PDB) after the first atomic MP structure was elucidated in 1985. Since …

The Impact Of China’S Environmental And Trade Policies On U.S. Plastic And Paper Waste Exports, Savant Nzayiramya, John C. Beghin

Cornhusker Economics

This article documents major environmental and trade policy changes in China and their impact on plastic and paper waste trade between the U.S. and China and other trade partners. The article explains how U.S. plastic waste exports have been nearly annihilated by these policies. Paper waste trade is more resilient and has survived the policy shifts in China, although quite diminished.

The Sorghum Bicolor Root Exudate Sorgoleone Shapes Bacterial Communities And Delays Network Formation, Peng Wang, Yen Ning Chai, Rebecca Roston, Franck E. Dayan, Daniel P. Schachtman

Center for Plant Science Innovation: Faculty Publications

Primary and secondary metabolites exuded from roots are key drivers of root-soil microbe interactions that contribute to the structure and function of microbial communities. Studies with model plants have begun to reveal the complex interactions between root exudates and soil microbes, but little is known about the influence of specialized exudates from crop plants. The aims of this work were to understand whether sorgoleone, a unique lipophilic secondary benzoquinone exuded only from the root hairs of sorghum, influences belowground microbial community structure in the field, to assess the effect of purified sorgoleone on the cultured bacteria from field soils, and …

Assessing Treatment Integrity Of Parent‑To‑Parent Phone Support For Families Of Students With Emotional And Behavioral Disturbance, Kristin Duppong Hurley, Jennifer Farley, Jacqueline Huscroft D’Angelo

University of Nebraska Public Policy Center: Publications

Assessing treatment integrity is essential to understanding how well school-based interventions are delivered. The assessment of treatment integrity is especially challenging for interventions that provide one-on-one peer support over the phone. To address this gap, we explored treatment integrity approaches used for the Parent Connectors program, which provides parent-to-parent support via weekly phone calls to families of students receiving special education services for emotional and behavioral disturbance. Our multi-dimensional approach to assessing treatment integrity includes the consideration of dose, adherence, quality of service delivery, participant responsiveness to the intervention and program differentiation. We share and discuss data from a variety …

Maldi-Tof Ms Biomarker Detection Models To Distinguish Rtx Toxin Phenotypes Of Moraxella Bovoculi Strains Are Enhanced Using Calcium Chloride Supplemented Agar, Matthew M. Hille, Michael L. Clawson, Aaron M. Dickey, Justin H. Lowery, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is the bacterium most often cultured from ocular lesions of cattle with infectious bovine keratoconjunctivitis, also known as bovine pinkeye. Some strains of M. bovoculi contain operons encoding for a repeats-in-toxin (RTX) toxin, which is a known virulence factor of multiple veterinary pathogens. We explored the utility of MALDI-TOF MS and biomarker detection models to classify the presence or absence of an RTX phenotype in M. bovoculi. Ninety strains that had undergone whole genome sequencing were classified by the presence or absence of complete RTX operons and confirmed with a visual assessment of hemolysis on blood agar. Strains …

Food Sourcing And The Relationship To Height Of 6th To 7th Graders In Southern Ethiopia And Zambia, Fulxen Ignacio Bescansa Luers

NUTR/GLST 498b: Global Research Experiences in Nutrition and Health

Introduction: Zambia and Ethiopia are Sub-Saharan African countries experiencing high rates of malnutrition and stunting. This study examines food sourcing and its relationship to stunting.

Methods: Anthropometric assessment and food frequency-sourcing interviews were taken for 488 6th-7th graders in Livingstone, Zambia and Hawassa, Ethiopia. Data were analyzed using ANOVA.

Results: Most students sourced food from local markets and home gardens; however Zambian students used a greater variety of sources. Those sourcing from home gardens were significantly taller.

Discussion: Home gardening provides economic and health benefits, as well as food security. A focus on preservation and use …

Relational Dialectics In College Ldrs: Managing The Tensions Of Long-Distance Dating In College, Kenessa Copeland

Honors Program: Senior Projects (Public)

A significant population of college students have been, or are currently, in a long distance relationship (LDR). This study examined tensions experienced by college students in LDRs using Relational Dialectics Theory (RDT) and Dark Side Theory. I used a thematic analysis to analyze responses from an open-ended survey distributed to and answered by 23 students in LDRs. I found that RDT was exemplified in the study by finding tensions of connection-autonomy and predictability-novelty, as well as tensions of balancing time and FOMO-living in the moment. The most common tension management strategies used were selection, separation, and reframing. Finally, I found …

Unconventional Agriculture: An Exploration Of Hydroponics And Aquaponics And Their Role As A Solution To Food Scarcity, Kai Meacham

Honors Program: Senior Projects (Public)

Unconventional agriculture includes methods of farming that are defined by unique farming practices and a focus on sustainability. Unconventional agriculture can take many forms, including hydroponic systems and aquaponic systems. These two systems are unique in the sense that they are soilless methods of growing plants. Both of these systems require careful monitoring because they do not have soil to act as a buffer and help maintain normal levels. Though these two systems are very similar they have some key differences in how they function, which ultimately leads to differing advantages and disadvantages of both systems. Additionally, these systems have …

Predictors Of Separation Anxiety In Dogs, Mckenna Yohe

Honors Program: Senior Projects (Public)

Understanding the potential causes and risk factors associated with separation anxiety in dogs is necessary to provide insight for possible prevention measures and to maintain the health and well-being of the animals affected by this condition. While a considerable amount of research has been done on this subject, it is still unclear what specific characteristics of dogs and their owners relate to the occurrence of separation anxiety and related behaviors. This study aimed to reexamine several dog and owner characteristics that have previously been evaluated in relation to the incidence of separation anxiety, as well as investigate how the frequency …

Free To Hate: Hate Crimes' Intertwinement With The Evolution Of Free Speech In The United States, Lee F. Paulson

Honors Program: Senior Projects (Public)

In response to the growing tension between civil liberties and civil rights, this research investigates the relationship between the relative expansiveness of free speech and a the nationwide propensity for hate crimes. I argue that government’s legal limitations of speech influence the development of linguistic and hierarchical norms in a national culture. Given structural inequality’s association to violence and crimes of intimidation, I hypothesize that as the government expands the legal bounds of free speech, the national propensity for hate crimes decreases. Text analyses of 50 influential freedom of expression rulings in the United States (U.S.) Supreme Court from 1919-2019 …

Take A Look Around: An Analysis Of Food Insecurity And Awareness On A College Campus, Allison Mcelroy

Honors Program: Senior Projects (Public)

The devastating hit from an unexpected global pandemic posed the question, who should we turn to? The opportunity of this thesis project motivated me to connect my passion for food and health with some source in the community around me. Not only did my own family and friends begin to struggle more with opportunities to work and provide, but many of my fellow university students did as well. I wanted to figure out how people on campus who were able, could physically participate in supporting those around them? The Husker Pantry on the University of Nebraska-Lincoln was brought to my …

A Person-Centered Care Model’S Effectiveness For Older Adults With Dementia: A Systematic Review And Meta-Analysis, Karen Goeschel

Honors Program: Senior Projects (Public)

I evaluated the effectiveness of person-centered care interventions for older adults with dementia. Quality of life and agitation levels were used as primary outcomes for the effectiveness of the intervention. Electronic databases were searched for studies which satisfied the inclusion principles and did not satisfy exclusion principles. Cluster-randomized trials and non-randomized control trials which compared person-centered care approaches to usual care were included. I performed two random-effects meta-analyses. Six studies with 1,384 patients were included. For older adults with dementia, person-centered care had no significant impact on quality-of-life improvement (SMD = -0.116, p = 0.206) or agitation reduction (SMD = …

An Analysis Of State Attorney General Attempts At Policy Influence Through Naag Multistate Advocacy Letters, Sam Crowley

Honors Program: Senior Projects (Public)

State Attorneys General have emerged as influential political actors on the national level in the last 25 years. State attorneys general seek to influence policy at the federal level in both partisan and bipartisan manners. Media attention in recent years and previous scholarship has focused mostly on areas of partisan conflict between and among state attorneys general. This paper seeks to explore areas of bipartisan cooperation among state attorneys general as demonstrated through the practice of signing multistate advocacy letters addressed to Congress, administrative agencies, and the private sector that are coordinated through the efforts of the National Association of …

Native. A Look Into Interior Design's Impact In Rural Communities., Ashlynn Engelhard

Honors Program: Senior Projects (Public)

Rural America faces a greater set of health challenges than ever before, especially when compared to its metropolitan counterparts. The Association of American Medical Colleges notes these rural disparities resulting from “economic, social, racial, ethnic, geographic, and health workforce factors.” Though somewhat daunting to consider, the AAMC concludes “We need to figure out how to use this data to target resources and interventions to make a meaningful difference in improving the health of rural America.”

Focusing on addressing rural disparities that inhibit communities from positive health and well-being, this projective design research project aims to provide a comprehensive intervention through …

Mediating Asian-Ness: How And Why Does Asian Identity Salience Vary By Biracial Status?, Kaitlan Wong

Honors Program: Senior Projects (Public)

The following study explores how and why Asian identity salience may vary between biracial and monoracial Asians. This study further aims to find potential mediators—including daily Asian contact, linked fate, group solidarity, and microaggressions—that might explain any group differences in Asian identity salience. I used the 2016 Post-Election National Asian American Survey to explore these research aims. Contrary to expectations, I found that biracial Asians have higher Asian identity salience than monoracial Asians. As expected, linked fate and microaggressions were positively associated with Asian identity salience. Surprisingly, daily Asian contact was negatively associated and group solidarity was not significantly associated …

The Interplay Of Emotion, Cognition, And Learning In The Language Classroom, Aleidine J. Moeller

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Challenge Statement Emotions are inextricably linked to our actions, behaviors, and dispositions. To promote deeper learning, emotion and cognition must be in sync to maximize learning. How can we connect our learners’ emotions in ways that fully capitalize on the interplay with cognition and engages them in the language learning process?

Abstract This article seeks to broaden the discourse on world language teaching to take a more holistic view of learning and teaching that supports and promotes the integration of feeling and thinking. A summary of the research on the role of emotions in learning is documented and classroom examples …

Rehabilitation For Anterior Cruciate Ligament Tears: A Written And Video Guide For Athletes Today And In The Future, Braeland Jones

Honors Program: Senior Projects (Public)

The following paper is a partner to a corresponding video exercise guide. The purpose of this paper is to support the exercises included in the guide with research. The research paper aids in defining which exercises are included in the first place as well as in which section they appear. The research is the literary match for the recorded exercise guide, which will be the visual side of the project. The two ideally should be read and watched together to fully understand content of both portions. The paper guide is split into three sections, evaluating the types of exercises and …

How Local School-Based Speech-Language Pathologists Facilitate Skill Transfer And Generalization For Students Who Stutter, Chloe Strong, Naomi Rodgers

Honors Program: Senior Projects (Public)

The purpose of this study was to determine what local school-based speech-language pathologists (SLPs) are doing to help their students who stutter generalize target communication skills beyond the therapy room. A survey was distributed to SLPs who work in the Lincoln Public Schools and 28 completed the survey. The survey covered the following topics: career background, self-efficacy in stuttering therapy, frequency of specific clinician practices, and goal setting and evaluating therapeutic progress. Results revealed that while treatment goals are being developed across the multiple domains impacted by stuttering, the majority of transfer activities focus on fluency techniques. Results also showed …

Organizational Cynicism, Dana Hollinshead, Megan Paul

Umbrella Summaries

What is organizational cynicism?

Organizational cynicism is defined as “a negative attitude toward one's employing organization, comprising three dimensions: (1) a belief that the organization lacks integrity; (2) negative affect toward the organization; and (3) tendencies to disparaging and critical behaviors toward the organization that are consistent with these beliefs and affect” (Dean et al., 1998, p. 345). Measures of organizational cynicism include statements such as “I’ve pretty much given up trying to make suggestions for improvements around here” (Tesluk et al., 1990, p. 283) and “I believe that [the organization] says one thing and does another” (Brandes et al., …

Defining And Validating Regenerative Farm Systems Using A Composite Of Ranked Agricultural Practices, Tommy L.D. Fenster, Claire E. Lacanne, Jacob R. Pecenka, Ryan B. Schmid, Michael M. Bredeson, Katya M. Busenitz, Alex M. Michels, Kelton D. Welch, Johnathan G. Lundgren

University of Nebraska State Museum: Entomology Papers

Background: Ongoing efforts attempt to define farms as regenerative to aid marketers, policymakers, farmers, etc. The approach needs to balance precision with function, and must be transparent, simple, scalable, transferable, incorruptible, and replicable. Methods: We developed practice-based scoring systems to distinguish regenerative cropland and rangeland, and validate them based on whether these scores scaled with regenerative goals on actual farm operations. Study systems included cornfields of the Upper Midwest, almond orchards of California, and rangeland systems of the Northern Plains. Response variables included soil carbon and organic matter, soil micronutrients, water infiltration rates, soil microbial communities, plant community structure, invertebrate …

The Chromosome-Level Genome Of Dragon Fruit Reveals Whole-Genome Duplication And Chromosomal Co-Localization Of Betacyanin Biosynthetic Genes, Jinfang Zheng, Lyndel W. Meinhardt, Ricardo Goenaga, Dapeng Zhang, Yanbin Yin

Food for Health: Publications

Dragon fruits are tropical fruits economically important for agricultural industries. As members of the family of Cactaceae, they have evolved to adapt to the arid environment. Here we report the draft genome of Hylocereus undatus, commercially known as the white-fleshed dragon fruit. The chromosomal level genome assembly contains 11 longest scaffolds corresponding to the 11 chromosomes of H. undatus. Genome annotation of H. undatus found ~29,000 protein-coding genes, similar to Carnegiea gigantea (saguaro). Whole-genome duplication (WGD) analysis revealed a WGD event in the last common ancestor of Cactaceae followed by extensive genome rearrangements. The divergence …

Conservation Management Improves Agroecosystem Function And Resilience Of Soil Nitrogen Cycling In Response To Seasonal Changes In Climate, Lidong Li, Julie Konkel, Virginia L. Jin, Sean M. Schaeffer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Understanding how conservation agricultural management improves soil nitrogen (N) stability in the face of climate change can help increase agroecosystem productivity and mitigate runoff, leaching and downstream water quality issues. We conducted a 2-year field study in a 36-year-old rain-fed cotton production system to evaluate the impacts of changing climatic factors (temperature and precipitation) on soil N under conservation management, including moderate inorganic N fertilizer application (0 and 67 kg N ha⁻¹ ), winter cover crops (fallow; winter wheat, Triticum aestivum L.; hairy vetch, Vicia villosa Roth), and reduced tillage (no-till; disk tillage). Structural equation modeling (SEM) was used …

De Novo Assembly And Annotation Of The North American Bison (Bison Bison) Reference Genome And Subsequent Variant Identification, L K. Dobson, A Zimin, D Bayles, E Fritz-Waters, D Alt, S Olsen, J Blanchong, J Reecy, T Pl Smith, J N. Derr

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Genomic tools have improved the ability to manage bison populations and enhanced efforts to conserve this iconic species. These tools have been particularly useful for detecting introgression of cattle genome within bison herds but are limited by the need to use the cattle genome as a surrogate for mapping reads. This complicates efforts to distinguish the species of origin of chromosomal segments in individual bison at the genomic level. An assembly (Bison_UMD1.0) based on 75X genome coverage by Illumina and 454 reads was generated using the MaSuRCA assembler, generating a 2.81 Gigbases de novo reference genome from American bison. Comparison …

Variations In Bacterial Community Structure And Antimicrobial Resistance Gene Abundance In Cattle Manure And Poultry Litter, Biyensa Gurmessa, Amanda J. Ashworth, Yichao Yang, Mary Savin, Philip A. Moore Jr, Steven C. Ricke, Giuseppe Corti, Ester Foppa Pedretti, Stefania Cocco

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cattle manure and poultry litter are widely used as fertilizers as they are excellent sources of nutrients; however, potential adverse environmental effects exist during land applications, due to the release of zoonotic bacteria and antimicrobial resistance (AMR) genes. This study was conducted to understand linkages between physiochemical composition, bacterial diversity, and AMR gene presence of cattle manure and poultry litter using quantitative polymerase chain reaction to enumerate four AMR genes (ermB, sulI, intlI, and blactx-m-32), Illumina sequencing of the 16 S region, and analysis of physical and chemical properties. Principal coordinate analysis of Bray–Curtis distance revealed distinct bacterial community structures …

Isometric Exercises And Stretching Warm-Up Program For University String Musicians: An Intervention Study, Emma Porter

Honors Program: Senior Projects (Public)

In this study, the purpose of this investigation was to find the effects of reducing pain through combining isometric and stretching warm-up exercises for stringed instrument musicians. In Lee’s et al. (2013) study, it showed that about 75 % of musicians have work-related musculoskeletal disorders and experience pain because of playing. In this study, they also saw that string players have the highest prevalence of both musculoskeletal disorders and pain while playing (Lee et al., 2013). In Nawrocka’s et al. (2014) journal article, the study showed the correlation of more pain in musicians that did not meet the recommended criteria …

Evaluating Person-Centered Factors Associated With Brain–Computer Interface Access To A Commercial Augmentative And Alternative Communication Paradigm, Kevin Pitt, Jonathan S. Brumberg

Department of Special Education and Communication Disorders: Faculty Publications

Current BCI-AAC systems largely utilize custom-made software and displays that may be unfamiliar to AAC stakeholders. Further, there is limited information available exploring the heterogenous profiles of individuals who may use BCI-AAC. Therefore, in this study, we aimed to evaluate how individuals with amyotrophic lateral sclerosis (ALS) learned to control a motor-based BCI switch in a row-column AAC scanning pattern, and person-centered factors associated with BCI-AAC performance. Four individuals with ALS completed 12 BCI-AAC training sessions, and three individuals without neurological impairment completed 3 BCI-AAC training sessions. To assess person-centered factors associated with BCI-AAC performance, participants completed both initial and …

Maternal Vitamin And Mineral Supplementation And Rate Of Maternal Weight Gain Affects Placental Expression Of Energy Metabolism And Transport-Related Genes, Wellison J. S. Diniz, Lawrence P. Reynolds, Pawel P. Borowicz, Alison K. Ward, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

Maternal nutrients are essential for proper fetal and placental development and function. However, the effects of vitamin and mineral supplementation under two rates of maternal weight gain on placental genome-wide gene expression have not been investigated so far. Furthermore, biological processes and pathways in the placenta that act in response to early maternal nutrition are yet to be elucidated. Herein, we examined the impact of maternal vitamin and mineral supplementation (from pre-breeding to day 83 post-breeding) and two rates of gain during the first 83 days of pregnancy on the gene expression of placental caruncles (CAR; maternal placenta) and cotyledons …

Advertising During The Covid-19 Global Pandemic: The Rise Of Influencer Marketing Strategies In A Recession, Bre Tenhulzen

Honors Program: Senior Projects (Public)

The COVID-19 pandemic reached the United States in January of 2020 and has since impacted nearly all industries, including advertising. Consumers, the guiding factors for all advertising strategies, faced drastic lifestyle changes and behavior shifts which sent current strategies askew. The virus has created insurmountable unknowns, thus advertising and marketing experts must rely on historical and recent data to develop strategies for brands forging ahead. COVID-19 is the first global crisis in the age of social media which has both helped and hindered communication during the crisis. Additionally, emerging influencer marketing strategies are proving more effective than ever as brands …

Exploiting Pull-In/Pull-Out Hysteresis In Electrostatic Mems Sensor Networks To Realize A Novel Sensing Continuous-Time Recurrent Neural Network, Mohammad H. Hasan, Amin Abbasalipour, Hamed Nikfarjam, Siavash Pourkamali, Muhammad Emad-Un-Din, Roozbeh Jafari, Fadi Alsaleem

Durham School of Architectural Engineering and Construction: Faculty Publications

The goal of this paper is to provide a novel computing approach that can be used to reduce the power consumption, size, and cost of wearable electronics. To achieve this goal, the use of microelectromechanical systems (MEMS) sensors for simultaneous sensing and computing is introduced. Specifically, by enabling sensing and computing locally at the MEMS sensor node and utilizing the usually unwanted pull in/out hysteresis, we may eliminate the need for cloud computing and reduce the use of analog-to-digital converters, sampling circuits, and digital processors. As a proof of concept, we show that a simulation model of a network of …