Digital Commons Network^™

Special Issue “Cfd Modeling Of Complex Chemical Processes: Multiscale And Multiphysics Challenges”, Li Xi, De-Wei Yin, Jae Sung Park

Department of Mechanical and Materials Engineering: Faculty Publications

No abstract provided.

Identification Of A Chlorovirus Pbcv-1 Protein Involved In Degrading The Host Cell Wall During Virus Infection, Irina V. Agarkova, Leslie C. Lane, David D. Dunigan, Cristian F. Quispe, Garry A. Duncan, Elad Milrot, Abraham Minsky, Ahmed Esmael, Jayadri S. Ghosh, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Chloroviruses are unusual among viruses infecting eukaryotic organisms in that they must, like bacteriophages, penetrate a rigid cell wall to initiate infection. Chlorovirus PBCV-1 infects its host, Chlorella variabilis NC64A by specifically binding to and degrading the cell wall of the host at the point of contact by a virus-packaged enzyme(s). However, PBCV-1 does not use any of the five previously characterized virus-encoded polysaccharide degrading enzymes to digest the Chlorella host cell wall during virus entry because none of the enzymes are packaged in the virion. A search for another PBCV-1-encoded and virion-associated protein identified protein A561L. The fourth domain …

Categorization Of Orthologous Gene Clusters In 92 Ascomycota Genomes Reveals Functions Important For Phytopathogenicity, Daniel Peterson, Tang Li, Ana M. Calvo, Yanbin Yin

Food for Health: Publications

Phytopathogenic Ascomycota are responsible for substantial economic losses each year, destroying valuable crops. The present study aims to provide new insights into phytopathogenicity in Ascomycota from a comparative genomic perspective. This has been achieved by categorizing orthologous gene groups (orthogroups) from 68 phytopathogenic and 24 non-phytopathogenic Ascomycota genomes into three classes: Core, (pathogen or non-pathogen) group-specific, and genome-specific accessory orthogroups. We found that (i) ~20% orthogroups are group-specific and accessory in the 92 Ascomycota genomes, (ii) phytopathogenicity is not phylogenetically determined, (iii) group-specific orthogroups have more enriched functional terms than accessory orthogroups and this trend is particularly evident in phytopathogenic …

Europium Sulfide Nanoprobes Predict Antiretroviral Drug Delivery Into Hiv-1 Cell And Tissue Reservoirs, Jonathan Herskovitz, Mahmudul Hasan, Jatin Machhi, Insiya Mukadam, Brendan M. Ottemann, James R. Hilaire, Christopher Woldstad, Joellyn Mcmillan, Yutong Liu, Javier Seravalli, Anandakumar Sarella, Howard E. Gendelman, Bhavesh D. Kevadiya

Department of Biochemistry: Faculty Publications

Background: Delivery of long-acting nanoformulated antiretroviral drugs (ARVs) to human immunodeficiency virus type one cell and tissue reservoirs underlies next generation antiretroviral therapeutics. Nanotheranostics, comprised of trackable nanoparticle adjuncts, can facilitate ARV delivery through real-time drug tracking made possible through bioimaging platforms. Methods: To model HIV-1 therapeutic delivery, europium sulfide (EuS) nanoprobes were developed, characterized and then deployed to cells, tissues, and rodents. Tests were performed with nanoformulated rilpivirine (NRPV), a non-nucleoside reverse transcriptase inhibitor (NNRTI) used clinically to suppress or prevent HIV-1 infection. First, CD4+ T cells and monocyte-derived macrophages were EuS-treated with and without endocytic blockers to identify …

The Ungovernable Novel: Towards A New Political Imaginary, Joseph Turner

Department of English: Dissertations, Theses, and Student Research

The primary objective of my thesis is to provide an initial definition of what we could call the “ungovernable novel.” I borrow the concept of the “ungovernable” from the field of political theory, and I apply it to the theory of the novel by way of an engagement of Mikhail Bakhtin’s and Georg Lukács’ theories of the novel. Building on this theoretical foundation, I argue that our contemporary political imagination has reached a historical juncture: we must abandon the dystopian framework that we have inherited from the Cold War, and we must move in the direction of the ungovernable novel. …

A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation And Alters Cell Identity, Renee M. Mcfee, Sarah M. Romereim, Alexandria P. Snider, Adam F. Summers, William E. Pohlmeier, Scott G. Kurz, Robert A. Cushman, John S. Davis, Jennifer R. Wood, Andrea S. Cupp

School of Veterinary and Biomedical Sciences: Faculty Publications

A naturally occurring bovine model with excess follicular fluid androstenedione (High A4), reduced fertility, and polycystic ovary syndrome (PCOS)-like characteristics has been identified. We hypothesized High A4 granulosa cells (GCs) would exhibit altered cell proliferation and/or steroidogenesis. Microarrays of Control and High A4 GCs combined with Ingenuity Pathway Analysis indicated that High A4 GCs had cell cycle inhibition and increased expression of microRNAs that inhibit cell cycle genes. Granulosa cell culture confirmed that A4 treatment decreased GC proliferation, increased anti-Müllerian hormone, and increased mRNA for CTNNBIP1. Increased CTNNBIP1 prevents CTNNB1 from interacting with members of the WNT signaling pathway thereby …

Early Satellite Cell Communication Creates A Permissive Environment For Long-Term Muscle Growth, Kevin A. Murach, Bailey D. Peck, Robert A. Policastro, Ivan J. Vechetti, Douglas W. Van Pelt, Cory M. Dungan, Lance T. Denes, Xu Fu, Camille R. Brightwell, Gabriel E. Zentner, Esther E. Dupont-Versteegden, Christopher I. Richards, Jeramiah J. Smith, Christopher S. Fry, John J. Mccarthy, Charlotte A. Peterson

Department of Nutrition and Health Sciences: Faculty Publications

Using in vivo muscle stem cell (satellite cell)-specific extracellular vesicle (EV) tracking, satellite cell depletion, in vitro cell culture, and single-cell RNA sequencing, we show satellite cells communicate with other cells in skeletal muscle during mechanical overload. Early satellite cell EV communication primes the muscle milieu for proper long-term extracellular matrix (ECM) deposition and is sufficient to support sustained hypertrophy in adult mice, even in the absence of fusion to muscle fibers. Satellite cells modulate chemokine gene expression across cell types within the first few days of loading, and EV delivery of miR 206 to fibrogenic cells represses Wisp1 expression …

Cultural Bereavement And Resilience In Refugee Resettlement: A Photovoice Study With Yazidi Women In The Midwest United States, Julie A. Tippens, Kaitlin Roselius, Irene Padasas, Gulie Khalaf, Kara Kohel, Elizabeth Mollard, Izdihar (Vianne) Sheikh

Department of Child, Youth, and Family Studies: Faculty Publications

This study explored how ethnic Yazidi refugee women overcome adversity to promote psychosocial health and well-being within the context of U.S. resettlement. Nine Yazidi women participated in two small photovoice groups, each group lasting eight sessions (16 sessions total). Women discussed premigration and resettlement challenges, cultural strengths and resources, and strategies to overcome adversity. Yazidi women identified trauma and perceived loss of culture as primary stressors. Participants’ resilience processes included using naan (as sustenance and symbol) to survive and thrive as well as by preserving an ethnoreligious identity. Findings suggest that women’s health priorities and resilience-promoting strategies center on fostering …

Saturated Free Fatty Acids Induce Placental Trophoblast Lipoapoptosis, Sathish Kumar Natarajan, Taylor Bruett, Philma Glora Muthuraj, Prakash K. Sahoo, Jillian Power, Justin L. Mott, Corrine Hanson, Ann Anderson-Berry

Department of Nutrition and Health Sciences: Faculty Publications

Obesity during pregnancy increases the risk for maternal complications like gestational diabetes, preeclampsia, and maternal inflammation. Maternal obesity also increases the risk of childhood obesity, intrauterine growth restriction (IUGR) and diabetes to the offspring. Increased circulating free fatty acids (FFAs) in obesity due to adipose tissue lipolysis induces lipoapoptosis to hepatocytes, cholangiocytes, and pancreatic-β-cells. During the third trimester of human pregnancy, there is an increase in maternal lipolysis and release of FFAs into the circulation. It is currently unknown if increased FFAs during gestation as a result of maternal obesity cause placental cell lipoapoptosis. Increased exposure of FFAs during maternal …

Crispr/Cas9-Induced Fad2 And Rod1 Mutations Stacked With Fae1 Confer High Oleic Acid Seed Oil In Pennycress (Thlaspi Arvense L.), Brice A. Jarvis, Trevor B. Romsdahl, Michaela G. Mcginn, Tara J. Nazarenus, Edgar B. Cahoon, Kent D. Chapman, John C. Sedbrook

Department of Biochemistry: Faculty Publications

Pennycress (Thlaspi arvense L.) is being domesticated as an oilseed cash cover crop to be grown in the off-season throughout temperate regions of the world. With its diploid genome and ease of directed mutagenesis using molecular approaches, pennycress seed oil composition can be rapidly tailored for a plethora of food, feed, oleochemical and fuel uses. Here, we utilized Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology to produce knockout mutations in the FATTY ACID DESATURASE2 (FAD2) and REDUCED OLEATE DESATURATION1 (ROD1) genes to increase oleic acid content. High oleic acid (18:1) oil is valued for its oxidative stability that is …

Process Interventions For Improving The Microbiological Safety Of Low Moisture Food Ingredients, Tushar Verma

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The recurrence of Salmonella in low moisture foods and the implementation of the FSMA rule requires a need to validate legacy and novel processing technologies. In this dissertation, a legacy thermal (extrusion), a novel thermal (radiofrequency (RF) heating), and a non-thermal (chlorine dioxide) technology, were evaluated as intervention technologies for Salmonella in low moisture foods. The twin-screw extruder was performed at different levels of screw speeds, temperatures, moisture contents, and fat contents to understand the impact of processing conditions on Salmonella inactivation in oat flour. At temperature >65°C, the Salmonella population was below the detection limit. At 55°C, Salmonella reduction …

Preclinical Development Of Single Walled Carbon Nanotube-Based Optical Biosensors, Eric M. Hofferber

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

High resolution, long-term monitoring of key biological analytes would improve patient outcomes by providing earlier detection of disease states and improved efficacy of treatment. One class of biosensors that have gained much attention in recent years are optical biosensors. Optical probes are attractive biosensors due to their noninvasive nature of detection, as certain light can pass through tissue, water, and blood. Single walled carbon nanotubes (SWNT) are a specific type of optical biosensor that fluoresce in the near infrared range of the electromagnetic spectrum and offer unparalleled spatial and temporal resolution. SWNT have been applied as biosensors in vitro, …

Improving Accuracy Of Unmanned Aerial System Thermal Infrared Remote Sensing For Use In Energy Balance Models In Agriculture Applications, Mitchell S. Maguire, C.M.U. Neale, Wayne E. Woldt

Department of Mechanical and Materials Engineering: Faculty Publications

Unmanned aerial system (UAS) remote sensing has rapidly expanded in recent years, leading to the development of several multispectral and thermal infrared sensors suitable for UAS integration. Remotely sensed thermal infrared imagery has been used to detect crop water stress and manage irrigation by leveraging the increased thermal signatures of water stressed plants. Thermal infrared cameras suitable for UAS remote sensing are often uncooled microbolometers. This type of thermal camera is subject to inaccuracies not typically present in cooled thermal cameras. In addition, atmospheric interference also may present inaccuracies in measuring surface temperature. In this study, a UAS with integrated …

Seductive Details In Training, Megan Paul

Umbrella Summaries

What are seductive details?

In a learning environment, seductive details are interesting but unimportant details that are not necessary to achieve the instructional objective (Garner et al., 1989). The information may be tangentially related to the topic but is not relevant to the main teaching goal. Such details are often included for the purpose of making the topic more interesting and engaging. There are many potential types of seductive details; they can be visual, verbal, or aural—static or dynamic images (e.g., illustrations, photos, animations), written or spoken words (e.g., text, narration), or sounds (e.g., music; Sundararajan & Adesope, 2020).

Why …

Effects Of Farrowing Stall Layout And Number Of Heat Lamps On Sow And Piglet Behavior, Suzanne M. Leonard, Hongwei Xin, Tami M. Brown-Brandl, Brett C. Ramirez, Anna K. Johnson, Somak Dutta, Gary A. Rohrer

Department of Animal Science: Faculty Publications

Farrowing stalls are used in the United States swine industry to reduce pre-weaning piglet mortality, enable efficient individual animal management, and decrease facility construction and operating costs. The quantity and quality of space provided for sows and piglets in farrowing stalls are important economic and welfare considerations. To further explore the impacts of farrowing stall space allocation, a large-scale field study was conducted to compare sow and piglet behavior when housed in three farrowing stall layouts (TSL – traditional stall layout, ECSL – expanded creep area stall layout, ESCSL – expanded sow and creep area stall layout) with either one …

Yātrā: A Phenomenology Of Acculturation And Sojourner Experience Of Indian International Students In The U.S., Pankaj Amrut Desai

Department of Educational Administration: Dissertations, Theses, and Student Research

This dissertation study utilized Interpretative Phenomenological Analysis (IPA) to understand the acculturation and sojourner experience of undergraduate Indian international students in the U.S. Midwest. Focusing exclusively on the lived experience of these students, this study engaged Berry’s Fourfold Model of Acculturation (Berry, 1992, 1994) and the ABC Model of Acculturation as presented by Ward et al., (2001) to amplify the voices of three participants to bring forth the meaning they attach to their experiences. This study made use of the concept of Yātrā to signify the multidimensional journey that these international students undertake while migrating from their heritage culture in …

College Sports, Enter At Your Own Risk: An Overview Of The Ncaa Insurance Policies Available To Its Student-Athletes, Remington Slama

Nebraska Law Bulletin (Selected Issues)

The mission statement of the National Collegiate Athletics Association (NCAA) is “to govern competition in a fair, safe, equitable and sportsmanlike manner, and to integrate intercollegiate athletics into higher education so that the educational experience of the student-athlete is paramount.” It is clear to many that the NCAA has forgotten this mission long ago, and now many institutions use student-athletes as their cash cows raking in millions in revenue. These student-athletes put their bodies on the line for the NCAA and its member institutions, and in return for some, they receive free education. There are many arguments about whether this …

Non-Nutritive Suck Burst Pattern Stability In Extremely Premature Infants, Alejandra Marquez

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The development of non-nutritive suck (NNS) burst dynamics in preterm infants reflects the integrity of the brain and is used clinically to assess feeding readiness and orofacial motor development (Mizuno and Ueda, 2005). The application of NNS analytics in the present report represents one outcome measurement set that is part of an ongoing clinical trial involving extremely preterm infants (EPI’s,[GA]) randomized to receive either pulsed orocutaneous stimulation therapeutics or a sham (blind pacifier), in conjunction with salivary sampling twice weekly to map gene expression of key proteins involved in neural development and molecular sensing of feeding related pathways in the …

A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation And Alters Cell Identity, Renee Mcfee Fee, Sarah Romereim, Alexandria P. Snider, Adam F. Summers, William E. Pohlmeier, Scott G. Kurz, Robert A. Cushman, John S. Davis, Jennifer R. Wood, Andrea S. Cupp

Department of Animal Science: Faculty Publications

A naturally occurring bovine model with excess follicular fluid androstenedione (High A4), reduced fertility, and polycystic ovary syndrome (PCOS)-like characteristics has been identified. We hypothesized High A4 granulosa cells (GCs) would exhibit altered cell proliferation and/or steroidogenesis. Microarrays of Control and High A4 GCs combined with Ingenuity Pathway Analysis indicated that High A4 GCs had cell cycle inhibition and increased expression of microRNAs that inhibit cell cycle genes. Granulosa cell culture confirmed that A4 treatment decreased GC proliferation, increased anti-Müllerian hormone, and increased mRNA for CTNNBIP1. Increased CTNNBIP1 prevents CTNNB1 from interacting with members of the WNT signaling pathway thereby …

Zara Strategic Analysis, Jessica Ha

Honors Program: Senior Projects (Public)

Zara is the Spanish flagship store for one of the world's largest and most successful fashion retailers, Inditex. The brand is an overarching global clothing brand with thousands of in-store locations and presence in e-commerce. This strategic audit on Zara will take a deeper look into the company's business model and strategies through an external and internal analysis. The analytic tools: Porter's Five Forces, PESTEL, and SWOT analyses are utilized to understand the fashion retail industry's competitive environment and how Zara sustains its leadership through its niche in fast fashion. Strategic recommendations and implementation ideas are also extended with the …

Tales Of Love's Perseverance: Family Bereavement Stories As A Means To Investigating Impacts Of End-Of-Life Care On Sense-Making, Cassidy Taladay

Department of Communication Studies: Dissertations, Theses, and Student Research

The current study explored the stories of 25 participants who had lost an immediate loved one to a terminal illness one or more years ago. Through the lens of the retrospective storytelling heuristic of Communicated Narrative Sense-Making Theory (CNSM, Koenig Kellas, 2018), participants told their bereavement stories. Findings revealed seven themes of significant meanings, values, and beliefs that defined bereavement experiences which led to the development of a framework of three main types of stories told in bereavement which all centered on time: Past, Present, and Future. These stories reflected what was important to participants in their bereavement, such as …

Clinical Case Study: The Effects Of Real-Time Iemg Biofeedback On Facial Muscle Activation Patterns In A Child With Congenital Facial Palsy, Alyssa Molina

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Experimental findings are limited concerning efficacious procedures for facial reanimation following persistent idiopathic facial paralysis or paresis. Additional research examining the use of real-time integrated electromyography (iEMG) biofeedback for young children with persistent idiopathic facial paralysis is one approach to further understand the efficacy of electrophysiology biofeedback techniques in facial reanimation for this age group and etiology. iEMG research has applications in interventions for pathology of nerves and muscles affecting animation of the face. The proposed clinical case study will adopt real time iEMG visual biofeedback to facilitate facial reanimation in a preadolescent child. Quantitative measurements will determine orofacial muscle …

Allianz Life Strategic Audit, Evan Powell

Honors Program: Senior Projects (Public)

Allianz Life is a company that specializes in creating life insurance and annuity products to wholesale to financial professionals. The company is based out of Minneapolis, MN, and is a subsidiary of the parent company Allianz SE. Allianz SE is the 46th largest company in the world and ranks number one for assets managed by an insurance company. Allianz SE is based out of Munich, Germany. This report aims to learn more about Allianz Life through internal and external analysis. The following analytical tools will be included: PESTEL, Porter’s Five Forces, and SWOT analysis. These tools help to explain the …

Out Of This World Club!, Alec Miller, Katherine Osmundson

Honors Program: Expanded Learning Clubs

The purpose of Out of this World Club! was to make STEM and space less scary and intimidating, and display that anyone, even elementary students, can have fun learning about space. Each week consisted of learning about different planets in our solar system, their characteristics, and how they are similar or different from one another. The activities centered around creating something tangible that is representative of space. For example, the students designed asteroids with a rock and paint, created constellations out of toothpicks and mini marshmallows, and designed small rockets powered by blowing through a straw. A principal goal of …

Pacific Life Strategic Analysis, Jake Litel-Smith

Honors Program: Senior Projects (Public)

Pacific Life is one of the most recognizable names in life insurance. The company is known for its brand and consistent delivery on its promises for over 150 years. This study shows a comprehensive analysis of Pacific Life, including external and internal considerations. Porter’s Five Forces and PESTEL will provide a guide to understanding the environment outside of the company. A SWOT analysis will provide a better grasp on the company’s current market position. The analysis will conclude with an understanding of Pacific Life’s current strategy and recommendations for its future success.

Wisdom Narratives: Communicated Sense-Making In Emerging Adulthood Autoimmune Disease, Jacqueline Gunning

Department of Communication Studies: Dissertations, Theses, and Student Research

Under the umbrellas of Communicated Sense-making (CSM, Koenig Kellas & Kranstuber Horstman, 2015), the current study explored 25 stories of illness told by assigned female at birth individuals with autoimmune disease. Using both the Theory of Memorable Messages (ToMM, Cooke-Jackson & Rubinsky, 2021) and Communicated Narrative Sense-making Theory (CNSM, Koenig Kellas, 2018), findings reveal helpful and harmful interpersonal memorable messages received across their illness journeys, as well as lessons learned and wisdom gained from disruptive chronic illness onset at a young age. Participants identified messages of belief, support, and interest as helpful while navigating disease, whereas they saw messages of …

Microfluidic-Based Bacterial Molecular Computing On A Chip, Daniel P. Martins, Michael Taynnan Barros, Benjamin O'Sullivan, Ian Seymour, Alan O'Riordan, Lee Coffey, Joseph Sweeney, Sasitharan Balasubramaniam,

School of Computing: Faculty Publications

Biocomputing systems based on engineered bacteria can lead to novel tools for environmental monitoring and detection of metabolic diseases. In this paper, we propose a Bacterial Molecular Computing on a Chip (BMCoC) using microfluidic and electrochemical sensing technologies. The computing can be flexibly integrated into the chip, but we focus on engineered bacterial AND Boolean logic gate and ON-OFF switch sensors that produces secondary signals to change the pH and dissolved oxygen concentrations. We present a prototype with experimental results that shows the electrochemical sensors can detect small pH and dissolved oxygen concentration changes created by the engineered bacterial populations’ …

Considering Student Voices In Reading Intervention: Re-Conceptualizing And Rethinking Possibilities For High School Reading Through Arts-Based Educational Research, Angela Kramer

Department of Teaching, Learning, and Teacher Education: Theses and Other Student Research

This study examines best practices for secondary literacy intervention and instruction. It also exemplifies the ability of Arts Based Educational Research to give voice to struggling and striving high school readers. This dissertation encompasses three time horizons. The first was the crafting of a ‘non-fiction fiction ‘ play (Goldenstein, 2013; Sizer, 1984). The play’s characters, setting, and plot, were built on my review of literature, and years working with reading students as both a general and special education teacher. The second phase, inspired by the Chicana feminist epistemology process of testimonio, involved a student centered read through and revision of …

Changes In Subcellular Localization Of Host Proteins Induced By Plant Viruses, Rosalba Rodriguez-Pena, Kaoutar El Mounadi, Hernan Garcia-Ruiz

Department of Plant Pathology: Faculty Publications

Viruses are dependent on host factors at all parts of the infection cycle, such as translation, genome replication, encapsidation, and cell-to-cell and systemic movement. RNA viruses replicate their genome in compartments associated with the endoplasmic reticulum, chloroplasts, and mitochondria or peroxisome membranes. In contrast, DNA viruses replicate in the nucleus. Viral infection causes changes in plant gene expression and in the subcellular localization of some host proteins. These changes may support or inhibit virus accumulation and spread. Here, we review host proteins that change their subcellular localization in the presence of a plant virus. The most frequent change is the …

Supporting The Child Welfare Workforce During The Coronavirus Pandemic: Provision Of Personal Protective Equipment, Quality Improvement Center For Workforce Development

Other QIC-WD Products

At the beginning of the Coronavirus pandemic some child welfare jurisdictions required continued, in-person contact with children and families, and workers struggled to obtain appropriate personal protective equipment (PPE), including masks, gloves, gowns, hand sanitizers, to protect themselves (and their families), as well as their clients. In March of 2020, the National Council on Juvenile Crime and Delinquency conducted a survey of juvenile justice and child welfare agencies in 20 states on agency response to COVID-19. Child welfare agency respondents reported that inadequate PPE was the most significant challenge they faced at that point in the pandemic. Survey recommendations included …