Digital Commons Network^™

Determination Of Freedom-From-Rabies For Small Indian Mongoose Populations In The United States Virgin Islands, 2019–2020, A. Springer Browne, Hannah M. Cranford, Clint N. Morgan, James A. Ellison, Are Berentsen, Nicholas Wiese, Alexandra Medley, John Rossow, Leanne Jankelunas, Alan S. Mckinley, Claudia D. Lombard, Nicole F. Angeli, Thomas Kelley, Jennifer Valiulus, Bethany Bradford, Valicia J. Burke-France, Cosme J. Harrison, Irene Guendel, Marissa Taylor, Gerard L. Blanchard, Jeffrey B. Doty, David J. Worthington, David Horner, Keith R. Garcia, Joseph Roth, Brett R. Ellis, Kristine M. Bisgard, Ryan Wallace, Esther M. Ellis

United States Department of Agriculture Wildlife Services: Staff Publications

Mongooses, a nonnative species, are a known reservoir of rabies virus in the Caribbean region. A cross-sectional study of mongooses at 41 field sites on the US Virgin Islands of St. Croix, St. John, and St. Thomas captured 312 mongooses (32% capture rate). We determined the absence of rabies virus by antigen testing and rabies virus exposure by antibody testing in mongoose populations on all three islands. USVI is the first Caribbean state to determine freedom-from-rabies for its mongoose populations with a scientifically-led robust cross-sectional study. Ongoing surveillance activities will determine if other domestic and wildlife populations in USVI are …

Testing A Bite-Shortened Hook To Minimize Fish Handling In A Recreational Fishery, Holden E. Harris, Brian K. Whalen, Andrew G. Gude, Michael S. Allen

United States Department of Agriculture Wildlife Services: Staff Publications

Discard mortality can make fishing unsustainable, even in catch-and-release or highly regulated fisheries. However, fishing practices and gears that minimize hook injury, handling, and air exposure can considerably improve fitness and survival in released fish. This study tested whether modified hooks could allow anglers to successfully land and then release fish in the water and without handling. Standard, barbless, and bite-shortened hooks were used to catch Spotted Seatrout Cynoscion nebulosus (n > 75 per hook type), and logistic models were used to assess the differences in landing success and hook self-release success by hook type and categorical fish size. Average landing …

Development And First Evaluation Of An Attractant Impregnated Adhesive Tape Against Blood-Sucking Flies, Junwei J. Zhu, Gwang-Hyun Roh, Yosuke Asamoto, Jen-Chieh Liu, Alexander Lehmann, Kyle Harrison, David B. Taylor, Hironao Otake

Department of Entomology: Faculty Publications

Stable flies are one of the most important arthropod pests of livestock that reduce cattle weight gain and milk production leading to annual economic losses in excess of $2 billion to the US cattle industry. The host-seeking behavior is primarily mediated by associated odors from stable fly larval development environments and host animals. The present paper reports the development and evaluation of attractant-impregnated adhesive tapes to reduce stable fly attacks on cattle. Laboratory bioassays showed that only m-cresol impregnated adhesive tapes caught significantly more stable flies (16 ± 1) than the control tape without attractant added (7 ± 1), with …

Stockpiling Versus Composting: Effectiveness In Reducing Antibiotic-Resistant Bacteria And Resistance Genes In Beef Cattle Manure, Zachery R. Staley, Bryan L. Woodbury, Bobbi S. Stromer, Amy Schmidt, Daniel D. Snow, Shannon Bartelt-Hunt, Bing Wang, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Manure storage methods can affect the concentration and prevalence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in cattle manure prior to land application. The objective of this study was to compare stockpiling and composting with respect to their effectiveness in reducing ARB and ARGs in beef cattle manure in a field-scale study. Field experiments were conducted in different seasons with different bulking agents for composting. For both the winter-spring cycle and the summer-fall cycle, ARB concentrations declined below the limit of quantification rapidly in both composting piles and stockpiles; however, ARB prevalence was significantly greater in the composting …

Landscape Of Stress: Tree Mortality Influences Physiological Stress And Survival In A Native Mesocarnivore, Jennifer R. Kordosky, Eric M. Gese, Craig M. Thompson, Patricia A. Terletzky, Lorin A. Neuman-Lee, Jon D. Schneiderman, Kathryn L. Purcell, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

Climate change and anthropogenic modifications to the landscape can have both positive and negative effects on an animal. Linking landscape change to physiological stress and fitness of an animal is a fundamental tenet to be examined in applied ecology. Cortisol is a glucocorticoid hormone that can be used to indicate an animal’s physiological stress response. In the Sierra Nevada Mountains of California, fishers (Pekania pennanti) are a threatened mesocarnivore that have been subjected to rapid landscape changes due to anthropogenic modifications and tree mortality related to a 4-year drought. We measured cortisol concentrations in the hair of 64 …

Risks Of Introduction And Economic Consequences Associated With African Swine Fever, Classical Swine Fever And Foot-And-Mouth Disease: A Review Of The Literature, Vienna Brown, Ryan S. Miller, Sophie C. Mckee, Karina H. Ernst, Nicole M. Didero, Rachel M. Maison, Meredith J. Grady, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

African swine fever (ASF), classical swine fever (CSF) and foot-and-mouth disease (FMD) are considered to be three of the most detrimental animal diseases and are currently foreign to the U.S. Emerging and re-emerging pathogens can have tremendous impacts in terms of livestock morbidity and mortality events, production losses, forced trade restrictions, and costs associated with treatment and control. The United States is the world's top producer of beef for domestic and export use and the world's third-largest producer and consumer of pork and pork products; it has also recently been either the world's largest or second largest exporter of pork …

Economic Losses Of Catfish To Avian Predation: A Case Report, Ganesh Kumar, Shraddha Hegde, David Wise, Charles Mischke, Brian Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Avian predation in catfish aquaculture has been a persistent issue throughout the history of the industry, and as production has expanded predation from piscivorous birds has intensified. Catfish ponds in the Mississippi River delta (in the Mississippi migratory flyway) provide a constant and readily accessible supply of forage for birds. Intensive foraging by fish-eating birds has led to a specific regulatory policy and numerous on-farm and regional management efforts. However, in 2016, legal challenges lead to recision of some federal policies and uncertainty as to allowable management, resulting in limitations on bird depredation. Estimating the extent of fish losses to …

Early Corn Stand Count Of Different Cropping Systems Using Uav-Imagery And Deep Learning, Chin Nee Vong, Lance S. Conway, Jianfeng Zhou, Newell R. Kitchen, Kenneth A. Sudduth

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Optimum plant stand density and uniformity is vital in order to maximize corn (Zea mays L.) yield potential. Assessment of stand density can occur shortly after seedlings begin to emerge, allowing for timely replant decisions. The conventional methods for evaluating an early plant stand rely on manual measurement and visual observation, which are time consuming, subjective because of the small sampling areas used, and unable to capture field-scale spatial variability. This study aimed to evaluate the feasibility of an unmanned aerial vehicle (UAV)-based imaging system for estimating early corn stand count in three cropping systems (CS) with different tillage and …

Addressing Work-Related Traumatic Stress Nebraska - Intervention Logic Model, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Each QIC-WD site developed a logic model to serve as a visual representation of their selected intervention. All logic models included four main components: inputs, activities, outputs, and outcomes. Collectively, these demonstrate the resources and actions required to implement the program, as well as the associated result or changes anticipated through implementation of the program. The hypothesized relationships are represented by the pathways connecting the listed activities and anticipated outcomes. For more information see Site Overview.

Onboarding Program Eastern Band Of Cherokee Indians - Intervention Overview, Quality Improvement Center For Workforce Development

Other QIC-WD Products

The QIC-WD coordinated with the Eastern Band of Cherokee Indians (EBCI) Family Safety Program (FSP) to create an onboarding program for child welfare social workers. The intervention was designed to address role clarity, social integration, and understanding of Cherokee history and culture for new hires. (For more information see the Site Overview.) The 5-week onboarding program included activities completed by the new employee, his/her supervisor, and other FSP team members. Onboarding program elements included:

• A structured, interactive review of FSP’s policies and procedures;
• A mock family case to illustrate the full case process;
• Content related to Cherokee culture, historical …

Supportive Supervision And Resiliency Ohio - Logic Model, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Each QIC-WD site developed a logic model to serve as a visual representation of their selected intervention. All logic models included four main components: inputs, activities, outputs, and outcomes. Collectively, these demonstrate the resources and actions required to implement the program, as well as the associated result of changes anticipated through implementation of the program. The hypothesized relationships are represented by the pathways connecting the listed activities and anticipated outcomes.

Supportive Supervision And Resiliency Ohio - Intervention Overview, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Coach Ohio, a multi-level supportive supervision intervention, was designed as part of the QIC-WD project to help child welfare staff within the six Ohio implementation counties prevent and mitigate the effects of burnout, secondary trauma, employee dissatisfaction, and disengagement from families and children served by the agencies (for more information see the Site Overview). Coach Ohio initially included two components: Resilience Alliance (RA) was developed by the New York City Administration of Children’s Services-New York University Children’s Trauma Institute to mitigate the effects of secondary trauma, create a healthier work environment for child welfare staff, and to help staff develop …

Mapping The Literature Of Health Care Management: An Update, Amber T. Burtis, Susan M. Howell, Mary K. Taylor

Copyright, Fair Use, Scholarly Communication, etc.

Objective: This study aims to identify the core journals cited in the health care management literature and to determine their coverage in the foremost bibliographic databases used by the discipline.

Methods: Using the methodology outlined by the Medical Library Association’s Nursing and Allied Health Resource Section (NAHRS) protocol for “Mapping the Literature of Nursing and Allied Health Professions,” this study updates an earlier study published in 2007. Cited references from articles published in a three-year range (2016–2018) were collected from five health care management journals. Using Bradford’s Law of Scattering, cited journal titles were tabulated and ranked according to the …

The Effect Of Heat Stress And Beta-Adrenergic Agonists On Fatty Acid Mobilization And Their Individual And Interacting Impact On The Adipose Transcriptome Of Ruminant Livestock, Rachel R. Reith

Department of Animal Science: Dissertations, Theses, and Student Research

Heat stress reduces livestock efficiency, induces inflammation, and alters adipose tissue metabolism through stress-induced epinephrine acting on beta-adrenoreceptors (β-AR). Supplementation of beta-adrenergic agonists (β-AA) improve livestock growth and carcass traits, also by acting on β-AR located in skeletal muscle and adipose tissue. The binding of β-AR in adipose tissue results in changes in adipose tissue metabolism including increased lipolysis and decreased fatty acid synthesis. Due to their similar effects on stress response pathways, the possible interaction of heat stress and β-AA could negatively impact animal well-being. The purpose of the first study was to understand how ractopamine (RAC), a beta-one …

Childhood Trauma And Substance Use: Differences By Race And Sex In Juvenile Justice Prevention Programs In Nebraska, Sophie Holtz

Honors Program: Senior Projects (Public)

This study seeks to analyze whether demographic factors such as gender and race have a relationship to the reporting of trauma symptoms in juveniles. This study also examines whether higher substance use has a relationship to higher reports of trauma symptoms. To gather this data, surveys were administered to juveniles involved in juvenile justice prevention programs across the state of Nebraska. Overall, we found that juvenile girls reported significantly higher amounts of trauma symptoms than boys do. There was also a significant difference in how much juvenile girls report using cannabis compared to juvenile boys. Furthermore, there was not a …

Fire Suppression And Ignition With Unmanned Aerial Vehicles, Carrick Detweiler, Sebastian Elbaum, James Higgins, Christian Laney, Craig Allen, Dirac L. Twidwell Jr, Evan Michale Beachly

School of Computing: Faculty Publications

An unmanned aerial vehicle (UAV) can be configured for fire suppression and ignition. In some examples, the UAV includes an aerial propulsion system, an ignition system, and a control system. The ignition system includes a container of delayed-ignition balls and a dropper configured by virtue of one or more motors to actuate and drop the delayed-ignition balls. The control system is configured to cause the UAV to fly to a site of a prescribed burn and, while flying over the site of the prescribed burn, actuate one or more of the delayed-ignition balls. After actuating the one or more delayed-ignition …

Handling Of Spurious Sequences Affects The Outcome Of High-Throughput 16s Rrna Gene Amplicon Profiling, Sandra Reitmeier, Thomas C. A. Hitch, Nicole Treichel, Nikolaos Fikas, Bela Hausmann, Amanda E. Ramer-Tait, Klaus Neuhaus, David Berry, Dirk Haller, Ilias Lagkouvardos, Thomas Clavel

Department of Food Science and Technology: Faculty Publications

16S rRNA gene amplicon sequencing is a popular approach for studying microbiomes. However, some basic concepts have still not been investigated comprehensively. We studied the occurrence of spurious sequences using defined microbial communities based on data either from the literature or generated in three sequencing facilities and analyzed via both operational taxonomic units (OTUs) and amplicon sequence variants (ASVs) approaches. OTU clustering and singleton removal, a commonly used approach, delivered approximately 50% (mock communities) to 80% (gnotobiotic mice) spurious taxa. The fraction of spurious taxa was generally lower based on ASV analysis, but varied depending on the gene region targeted …

Impact Of Maize Hormonal Interactions On The Performance Of Spodoptera Frugiperda In Plants Infected With Clavibacter Michiganensis Subsp. Nebraskensis, Karen Da Silva, Sydney E. Everhart, Joe Louis

Department of Entomology: Faculty Publications

In nature, plants interact with multiple organisms, below and aboveground. Although interactions of plants with single biotic stressors are well characterized, knowledge of how the immune system responds to multiple biotic stressors is lacking. It is known that the two most important pathways involved in the plant immune defenses are jasmonic acid (JA) and salicylic acid (SA). The crosstalk between these plant hormonal signaling pathways seems to fine-tune the plant responses to different stressors. In this study, we characterized maize (Zea mays) hormonal interactions under the attack of two economically important pests: the bacterial pathogen that causes Goss’s …

The Value Of Republishing Scientific Literature In Institutional Repositories, Sue Ann Gardner, Linnea Fredrickson, Paul Royster

University of Nebraska-Lincoln Libraries: Presentations

Background: The University of Nebraska-Lincoln University Libraries institutional repository was founded in 2005 on the Digital Commons platform. It currently contains more than 118,000 full-text scholarly works that have been downloaded over 73,000,000 times.

Problem that drove the project: Older scientific literature may only occasionally circulate from university library collections or be purchased by readers from remaindered publishers' stock.

Methods: This research will employ statistical analyses to demonstrate the value of making available four series of scientific literature in the University of Nebraska-Lincoln University Libraries Digital Commons: Insecta Mundi, the Nebraska Bird Review, the Transactions of the Nebraska …

Approaches For And Barriers To The Long-Term Execution Of A Recovery-Oriented Rehabilitation Model Of Treatment In A Community Day Psychiatric Rehabilitation Setting, Sarah Fischer

Department of Psychology: Dissertations, Theses, and Student Research

This dissertation explores the process of creating, implementing, adapting and evaluating a program of care that is composed of specific program features that promote the acquisition of independent living skills, prosocial behavior, progress toward personal recovery goals, and more integrated community living. This program was created and implemented at a community mental health agency that provides integrated mental health care services for individuals with serious mental illness (SMI).

The goals of this dissertation were to (1) compare initial and long-term fidelity to the psychiatric rehabilitation model in order to establish the program’s ability to prevent drift, (2) explore the culture …

Robotic Technologies For High-Throughput Plant Phenotyping: Contemporary Reviews And Future Perspectives, Abbas Atefi, Yufeng Ge, Santosh Pitla, James Schnable

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Phenotyping plants is an essential component of any effort to develop new crop varieties. As plant breeders seek to increase crop productivity and produce more food for the future, the amount of phenotype information they require will also increase. Traditional plant phenotyping relying on manual measurement is laborious, time-consuming, error-prone, and costly. Plant phenotyping robots have emerged as a high-throughput technology to measure morphological, chemical and physiological properties of large number of plants. Several robotic systems have been developed to fulfill different phenotyping missions. In particular, robotic phenotyping has the potential to enable efficient monitoring of changes in plant traits …

Comparison Of Promoter, Dna Vector, And Cationic Carrier For Efficient Transfection Of Hmscs From Multiple Donors And Tissue Sources, Tyler Kozisek, Andrew Hamann, Luke Samuelson, Miguel Fudolig, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be enhanced through genetic modification. However, gene delivery, particularly through nonviral routes, is inefficient. To address the shortcomings of nonviral gene delivery to hMSCs, our lab has previously demonstrated that pharmacological “priming” of hMSCs with clinically approved drugs can increase transfection in hMSCs by modulating transfection-induced cytotoxicity. However, even with priming, hMSC transfection remains inefficient for clinical applications. This work takes a complementary approach to addressing the challenges of transfecting hMSCs by systematically investigating key transfection parameters for their effect on transgene expression. Specifically, we investigated …

Effect Of Demographic And Health Dynamics On Cognitive Status In Mexico Between 2001 And 2015: Evidence From The Mexican Health And Aging Study, Silvia Mejia-Arango, Jaqualine Avila, Brian Downer, Marc A. Garcia, Alejandra Michaels-Obregon, Joseph L. Saenz, Rafael Samper-Ternent, Rebeca Wong

Department of Sociology: Faculty Publications

Sources of health disparities such as educational attainment, cardiovascular risk factors, and access to health care affect cognitive impairment among older adults. To examine the extent to which these counteracting changes affect cognitive aging over time among Mexican older adults, we examine how sociodemographic factors, cardiovascular diseases, and their treatment relate to changes in cognitive function of Mexican adults aged 60 and older between 2001 and 2015. Self and proxy respondents were classified as dementia, cognitive impairment no dementia (CIND), and normal cognition. We use logistic regression models to examine the trends in dementia and CIND for men and women …

Reference Transcriptomes Of Porcine Peripheral Immune Cells Created Through Bulk And Single-Cell Rna Sequencing, Juber Herrera-Uribe, Jayne E. Wiarda, Sathesh K. Sivasankaran, Lance Daharsh, Haibo Liu, Kristen A. Byrne, Timothy P. L. Smith, Joan K. Lunney, Crystal L. Loving, Christopher Tuggle

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Pigs are a valuable human biomedical model and an important protein source supporting global food security. The transcriptomes of peripheral blood immune cells in pigs were defined at the bulk cell-type and single cell levels. First, eight cell types were isolated in bulk from peripheral blood mononuclear cells (PBMCs) by cell sorting, representing Myeloid, NK cells and specific populations of T and B-cells. Transcriptomes for each bulk population of cells were generated by RNA-seq with 10,974 expressed genes detected. Pairwise comparisons between cell types revealed specific expression, while enrichment analysis identified 1,885 to 3,591 significantly enriched genes across all 8 …

Abusive Supervision, Megan Paul

Umbrella Summaries

What is abusive supervision?

Abusive supervision refers to “subordinates' perceptions of the extent to which supervisors engage in the sustained display of hostile verbal and nonverbal behaviors, excluding physical contact” (Tepper, 2000, p. 178). Abusive supervision is most commonly measured using 15 items that describe abusive behaviors, and subordinates rate the frequency with which the supervisor engages in each behavior. Examples include “Ridicules me,” “Puts me down in front of others,” “Blames me to save himself/herself embarrassment,” and “Breaks promises he/she makes” (Tepper, 2000). Thus, subordinates do not judge whether they feel abused or consider their supervisors’ behavior abusive; they …

Creation And Application Of Various Tools For The Reconstruction, Curation, And Analysis Of Genome-Scale Models Of Metabolism, Wheaton L. Schroeder

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Systems biology uses mathematics tools, modeling, and analysis for holistic understanding and design of biological systems, allowing the investigation of metabolism and the generation of actionable hypotheses based on model analyses. Detailed here are several systems biology tools for model reconstruction, curation, analysis, and application through synthetic biology. The first, OptFill, is a holistic (whole model) and conservative (minimizing change) tool to aid in genome-scale model (GSM) reconstructions by filling metabolic gaps caused by lack of system knowledge. This is accomplished through Mixed Integer Linear Programming (MILP), one step of which may also be independently used as an additional curation …

Carotenoid-Rich Brain Nutrient Pattern Is Positively Correlated With Higher Cognition And Lower Depression In The Oldest Old With No Dementia, Jirayu Tanprasertsuk, Tammy M. Scott, Aron K. Barbey, Kathryn Barger, Xiang-Dong Wang, Mary Ann Johnson, Leonard W. Poon, Rohini Vishwanathan, Nirupa R. Matthan, Alice H. Lichtenstein, Guylaine Ferland, Elizabeth R. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Background: Healthy dietary patterns are related to better cognitive health in aging populations. While levels of individual nutrients in neural tissues are individually associated with cognitive function, the investigation of nutrient patterns in human brain tissue has not been conducted.

Methods: Brain tissues were acquired from frontal and temporal cortices of 47 centenarians from the Georgia Centenarian Study. Fat-soluble nutrients (carotenoids, vitamins A, E, K, and fatty acids [FA]) were measured and averaged from the two brain regions. Nutrient patterns were constructed using principal component analysis. Cognitive composite scores were constructed from cognitive assessment from the time point closest to …

Soil Quality Assessment Of An Agroforestry System Following Long-Term Management In The Ozark Highlands, Shane Ylagan, Helen C.S. Amorim, Amanda J. Ashworth, Tom Sauer, Brian J. Wienhold, Phillip R. Owens, Yuri L. Zinn, Kristofor R. Brye

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Soil Management Assessment Framework (SMAF) is a quantitative soil quality (SQ) evaluation tool that is widely applied to assess soil response to specific agricultural management practices over time. Considering the reported SQ benefits of agroforestry (AF) systems and the potential usefulness of SMAF, the objective of this study was to evaluate the effects of tree species (pecan [Carya illinoinensis (Wangenh.) K. Koch] and northern red oak [Quercus rubra L.]), soil fertility source (poultry litter [PL] and inorganic N fertilizer [control]), and soil depth (0–15 and 15–30 cm) on SMAF-derived SQ indices after 17 yr of management at an AF …

What’S The Big Deal? How Researchers Are Navigating Changes To Journal Access, Danielle Cooper, Oya Y. Rieger

Copyright, Fair Use, Scholarly Communication, etc.

The dominant mode by which research libraries have provided maximum journal access as cheaply as possible—subscription bundles or “Big Deals”—is giving way to new approaches. This transition is taking place through a combination of negotiations, activism, business modeling, user needs research, and decision support, among other factors. To support these processes, Ithaka S+R partnered with 11 academic libraries to understand researcher perceptions to help inform their ongoing strategic decision making about Big Deal journal subscriptions.

Recognizing that libraries must also undertake case-by-case assessments prior to making decisions about any particular journal package, in this report we share findings from the …

Sweet Sorghum Genotypes Tolerant And Sensitive To Nitrogen Stress Select Distinct Root Endosphere And Rhizosphere Bacterial Communities, Lucas Dantas Lopes, Yen Ning Chai, Ellen L. Marsh, John F. Rajewski, Ismail Dweikat, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The belowground microbiomes have many beneficial functions that assist plant growth, including nutrient cycling, acquisition and transport, as well as alleviation of stresses caused by nutrient limitations such as nitrogen (N). Here we analyzed the root endosphere, rhizosphere and soil bacterial communities of seven sweet sorghum genotypes differing in sensitivity to N-stress. Sorghum genotypes were grown in fields with no (low-N) or sufficient (high-N) N. The dry shoot weight ratio (low-N/high-N) was used to determine N-stress sensitivity. Our hypothesis was that genotypes tolerant and sensitive to N-stress select distinct bacterial communities. The endosphere and rhizosphere bacterial community structure were significantly …