Digital Commons Network^™

Toxicology Of Chemical Stress To Monarch Butterflies (Danaus Plexippus L.), Annie Krueger

Department of Entomology: Dissertations, Theses, and Student Research

Monarch butterfly (Danaus plexippus L.) population declines have caught the attention of the country and prompted nationwide conservation initiatives. The United States Fish and Wildlife Service has identified insecticide exposure and loss of milkweed (Asclepias spp.) reproductive habitat as primary threats to the monarch. In the Midwestern US, milkweed largely occurs around cropland borders where there may be a spatial and temporal overlap of monarch larvae, insecticide usage, and fertilizer applications. In this study, the acute toxicity and sub-lethal effects on growth and diet consumption of two commonly used pyrethroid insecticides, bifenthrin and beta-cyfluthrin, were characterized …

Getting Our Act(Ivism) Together: Understanding And Fostering Secondary And University Teacher Advocacy Collaborations, Nicole E. Green

Department of English: Dissertations, Theses, and Student Research

Given the deleterious effects on students and teachers caused by ever-expanding neoliberal approaches to K-16 English Language Arts and literacy education policy, this dissertation argues effective policy advocacy and reform absolutely depends on collaborations among secondary ELA and postsecondary composition and English education teacher-scholars. Borrowing from the traditions of participant action research, this project traces the experiences of a small group of secondary and postsecondary English educators across the span of a 16-month collaborative advocacy project. By examining a range of data including recordings of group meetings, interviews, and written reflections through the lens of activity theory, this study seeks …

Social And Ecological Correlates Of Avian Infection By Haemosporidian Blood Parasites, Ian R. Hoppe

School of Natural Resources: Dissertations, Theses, and Student Research

Haemosporidian parasites are a significant source of morbidity and mortality for birds. There is growing recognition of the negative consequences of haemosporidian infections for wild birds at individual and population levels. Avian haemosporidians are geographically widespread, have been detected from a phylogenetically diverse array of hosts, and have been the focus of extensive research due to their impacts on birds and their similarity to vector-borne diseases of humans. However, factors influencing haemosporidian transmission, especially transmission between species, are poorly understood. To better understand these influences, we compared prevalence and diversity of haemosporidian blood parasite infections among species in a behaviorally …

Perceptions Of Future Community And Individual Well-Being In Rural Nebraska, Amanda L. Kowalewski

Department of Agricultural Economics: Dissertations, Theses, and Student Research

Populations of rural areas continue to decline, yet some communities are more vibrant than ever. While past research has studied current satisfaction or well-being, few have examined future well-being. Using an ordinal logistic regression and combining primary and secondary data sources, this study investigates the predictors of rural Nebraskan’s sense of future well-being, both at the community and individual levels. The model indicates that resilience may be more important in well-being than social capital. Additionally, certain satisfaction indicators are more important than others. Factor analysis was employed to re-index variables, and findings were similar. Social capital, resilience, and quality of …

Host Cell Responses To Zika Virus Infection, Bikash Ranjan Sahoo

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The re-emergence of Zika virus (ZIKV) in 2015 as a significant human pathogen causing neurological diseases in infants as well as adults is a serious global health concern. A clear understanding of the mechanisms involved in ZIKV replication in infected host cells as well as the host responses to virus infection are keys to the development of therapeutic strategies against ZIKV. Studies conducted in this dissertation demonstrate that ZIKV infection induces the activation of mTOR signaling cascade that promotes viral protein accumulation and infectious progeny production. While both mTORC1 and mTORC2 are essential for ZIKV replication, the observation that depletion …

Nicarbazin As An Oral Contraceptive In Eared Doves, Lourdes Olivera, Silvia Pereyra, Georgget Banchero, Guillermo Tellechea, Jorge Sawchik, Michael L. Avery, Ethel Rodríguez

United States Department of Agriculture Wildlife Services: Staff Publications

Eared doves (Zenaida auriculata) are responsible for substantial losses in cereal and oil crops as well as in dairy and feedlot production in the southern cone of South America. Various strategies have been shown to be effective in reducing damage at the farm scale, but in some scenarios, it is necessary to also incorporate population control methods due to excessive bird population size. An alternative approach to reduce pest bird populations is the use of contraceptive methods, minimizing the impact on the environment and non-target populations. Nicarbazin is registered in the United States as a contraceptive for Branta …

The Sulfur Microbial Diet And Risk Of Colorectal Cancer By Molecular Subtypes And Intratumoral Microbial Species In Adult Men, Daniel R. Sikavi, Long H. Nguyen, Koichiro Haruki, Tomotaka Ugai, Wenjie Ma, Dong D. Wang, Kelsey N. Thompson, Yan Yan, Tobyn Branck, Jeremy E. Wilkinson, Naohiko Akimoto, Rong Zhong, Mai Chan Lau, Kosuke Mima, Keisuke Kosumi, Teppei Morikawa, Eric B. Rimm, Wendy S. Garrett, Jacques Izard, Yin Cao, Mingyang Song, Curtis Huttenhower, Shuji Ogino, Andrew T. Chan

Department of Food Science and Technology: Faculty Publications

Introduction: We recently described the sulfur microbial diet, a pattern of intake associated with increased gut sulfur-metabolizing bacteria and incidence of distal colorectal cancer (CRC). We assessed whether this risk differed by CRC molecular subtypes or presence of intratumoral microbes involved in CRC pathogenesis (Fusobacterium nucleatum and Bifidobacterium spp.).

Methods: We performed Cox proportional hazards modeling to examine the association between the sulfur microbial diet and incidence of overall and distal CRC by molecular and microbial subtype in the Health Professionals Follow-Up Study (1986-2012).

Results: We documented 1,264 incident CRC cases among 48,246 men, approximately 40% …

Avian Influenza A Virus Susceptibility, Infection, Transmission, And Antibody Kinetics In European Starlings, Jeremy W. Ellis, J. Jeffrey Root, Loredana M. Mccurdy, Kevin T. Bentler, Nicole L. Barrett, Kaci K. Vandalen, Katherine L. Dirsmith, Susan A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

Avian influenza A viruses (IAVs) pose risks to public, agricultural, and wildlife health. Bridge hosts are spillover hosts that share habitat with both maintenance hosts (e.g., mallards) and target hosts (e.g., poultry). We conducted a comprehensive assessment of European starlings (Sturnus vulgaris), a common visitor to both urban and agricultural environments, to assess whether this species might act as a potential maintenance or bridge host for IAVs. First, we experimentally inoculated starlings with a wild bird IAV to investigate susceptibility and replication kinetics. Next, we evaluated whether IAV might spill over to starlings from sharing resources with a …

Mesopredator Frugivory Has No Effect On Seed Viability And Emergence Under Experimental Conditions, John P. Draper, Trisha B. Atwood, Noelle G. Beckman, Karin M. Kettenring, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Members of the order Carnivora are a unique and important seed disperser who consume and deposit undamaged seeds while providing regular long-distance seed dispersal opportunities. Some members of Carnivora, such as coyotes (Canis latrans), are undergoing range expansions which may help the plant species they consume colonize new locations or replace dispersal services provided by recently extirpated species. In this study, we evaluated aspects of the seed dispersal effectiveness of coyotes and gut passage time to determine the potential dispersal distances for three commonly consumed and commonly occurring plant species (Amelanchier alnifolia, Celtis ehrenbergiana, and Juniperus osteosperma). We also investigated …

Sars-Cov-2 Concentrations In A Wastewater Collection System Indicated Potential Covid-19 Hotspots At The Zip Code Level, Renys E. Barrios, Chin Lim, Megan S. Kelley, Xu Li

Department of Nutrition and Health Sciences: Faculty Publications

Wastewater based epidemiology (WBE) has been successfully applied for SARS-CoV-2 surveillance at the city and building levels. However, sampling at the city level does not provide sufficient spatial granularity to identify COVID-19 hotspots, while data from building-level sampling are too narrow in scope for broader public health application. The objective of this study was to examine the feasibility of using wastewater from wastewater collection systems (WCSs) to monitor COVID-19 hotspots at the zip code level. In this study, 24-hr composite wastewater samples were collected from five manholes and two wastewater treatment plants (WWTPs) in the City of Lincoln, Nebraska. By …

Biofiltration Application At Ethanol Plants: Analysis Of Aqueous Streams And Treatment Of Vocs, Katie Marie Mowat

Department of Environmental Engineering: Theses and Other Student Research

The United States used 14.5 billion gallons of the biofuel ethanol in 2019 produced primarily (82%) in dry-mill corn ethanol plants. These plants produce volatile organic compounds (VOCs) — some of which are hazardous air pollutants (HAPs) — during production. Traditional treatment methods for gaseous emissions use a large quantity of water or natural gas. Thus, a bio-trickling filter (BTF) is considered an innovative alternative treatment method. A lab-scale BTF was used in this study to look at the effect of ethanol concentration and temperature on the treatment of a HAPs mixture. Gaseous and aqueous testing were performed on the …

Frontline Job Redesign Louisiana - Evaluation Overview, Quality Improvement Center For Workforce Development

Other QIC-WD Products

The QIC-WD evaluation was conducted with the support of the Louisiana Department of Children and Families to determine if a Job Redesign was effective in improving workforce and child welfare outcomes.

Research Questions

A key research question concerned the extent to which the job redesign reduced the amount of time caseworkers spent on administrative tasks and increased time spent on clinical work with children and families. The QIC-WD also tested questions related to the expected effects of the redesign on workers’ perceptions of their job complexity, role overload, and work-life balance, and how these perceptions relate to job satisfaction, case …

Competency-Based Personnel Selection Oklahoma - Intervention Overview, Quality Improvement Center For Workforce Development

Other QIC-WD Products

A Competency-based Personnel Selection process was the intervention designed as part of the QIC-WD project to address inconsistencies in hiring and to identify candidates with the desired competencies to be hired as Child Welfare Specialist (CWS) I/II with Oklahoma Human Services (OKDHS) (for more information see the Site Overview). The Competency-based Personnel Selection process is a standardized hiring procedure that includes a structured interview with behaviorally anchored rating scales, a typing assessment, and a writing assessment. Additionally, research was conducted on a set of academic and commercial hiring assessments to determine how well these measures of personality, cognitive skills, …

Onboarding Program Eastern Band Of Cherokee Indians - Evaluation Overview, Quality Improvement Center For Workforce Development

Other QIC-WD Products

The QIC-WD evaluation was conducted with the support of the Eastern Band of Cherokee Indians Family Safety Program (EBCI FSP) to determine if an Onboarding intervention was effective in improving workforce outcomes.

Research Questions

The evaluation of the newly developed onboarding program for the Eastern Band of Cherokee Indians Family Safety Program (EBCI FSP) was designed to understand both implementation and early outcomes. Examples of primary implementation questions from the new employee’s perspective included:

• To what degree was the new employee’s workspace ready on their first day?
• Did the new employee have individual meetings with their supervisor in weeks 1-5? …

The Role Of Fatty Acids In Ruminant Diets And Novel Feed Ingredients High In Omega-3 Fatty Acids Fed In Feedlot Diets, Mitchell M. Norman

Department of Animal Science: Dissertations, Theses, and Student Research

A finishing study evaluated the performance, carcass characteristics and fatty acid profiles of steers fed four inclusions of a novel feedstuff Green Grass. Green Grass is a product comprised of sesame meal, giant kelp, cassava, and sorghum containing high concentrations of polyunsaturated fatty acids. No differences were observed in initial BW, final BW, BW gain, HCW, LM area, 12^th rib fat depth, calculated YG, or liver abscess %. Dry matter intake linearly increased as Green Grass inclusion increased in the diet. Steers fed Green Grass had lower G:F than control cattle, and steers fed 30% Green Grass had a …

Afterschool Club: German For K-5, Justin Solheim

Honors Program: Expanded Learning Clubs

The lessons found in this paper were designed for a group of 20-25 elementary schools students in downtown Lincoln, NE. The students were split into two groups: K-2 and 3-5. Through a variety of activities, the students learned the basics of the German language and culture. Many of the lessons are simple and do not require difficult thinking, as this was a summer club. Instead, they allow students to collaborate, move around, color, and solve puzzles.

A Typology Of Drought Decision Making: Synthesizing Across Cases To Understand Drought Preparedness And Response Actions, Amanda E. Cravens, Jen Henderson, Jack Friedman, Nina Burkardt, Ashley E. Cooper, Tonya Haigh, Michael Hayes, Jamie Mcenvoy, Stephanie Paladino, Adam K. Wilke, Hailey Wilmer

National Drought Mitigation Center: Faculty Publications

Drought is an inescapable reality in many regions, including much of the western United States. With climate change, droughts are predicted to intensify and occur more frequently, making the imperative for drought management even greater. Many diverse actors – including private landowners, business owners, scientists, non-governmental organizations (NGOs), and managers and policymakers within tribal, local, state, and federal government agencies – play multiple, often overlapping roles in preparing for and responding to drought. Managing water is, of course, one of the most important roles that humans play in both mitigating and responding to droughts; but, focusing only on “water managers” …

Study Of Halide Gradient Formation Via Solution–Solid Halide Exchange In Crystalline Ch3Nh3Pbbr3 Thin Films, Behnaz Akbari

Department of Chemistry: Dissertations, Theses, and Student Research

In this thesis, attempts have been made to understand halide exchange and mass transport inside the solid lattice of Lead Halide Perovskites (LHPs). We aim for the transformation of MAPbBr₃ using ionic solutions including 1-Butyl-3-methylimidazolium iodide (BMIMI), and 1-Butyl-3-methylimidazolium chloride (BMIMCl) as halide ion sources to observe the halide diffusion across the thin film sample and to have LHPs with a gradient of mixed halides (MAPbBr_3-x (I/Cl)_x, 0 ≤ x ≤ 3). Successfully, MAPbBr₃ single-crystalline thin film was fabricated with different configurations. Surface analysis of the LHPs, elemental composition, halide diffusion, roughness, and size of …

Super Solar, William Wilton

Honors Program: Expanded Learning Clubs

No abstract provided.

Efficient Polyhydroxyalkanoate Production By Rhodopseudomonas Palustris From Lignocellulosic Biomass, Brandi Brown

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Polyhydroxyalkanoates (PHAs) are biopolymers produced by bacteria with the potential to replace conventional plastics. However, the relatively high production costs of PHAs are keeping them from market acceptance, with approximately half of the production costs derived from the feedstock. Thus, engineering a microbe for PHA production from cheaper and renewable carbon sources is necessary to promote the valorization of PHAs. Lignocellulosic biomass is considered to be one of the most economic carbon sources in the world, and is thus an attractive candidate for cheaper production of bioplastics. Rhodopseudomonas palustris CGA009 is a metabolically robust bacterium capable of catabolizing lignin breakdown …

Student Engagement In Higher Music Education With Online Learning Components: A Mixed Methods Case Study, Xinwei Liu

Glenn Korff School of Music: Dissertations, Theses, Student Creative Work, and Performances

Higher education underwent an unprecedented transformation from conventional face-to-face education to remote learning due to the COVID-19 pandemic. In the 2020-2021 academic year, most universities throughout the U.S. had moved educational programs online, so professors and instructors, with and without previous remote learning experience, were suddenly expected to shift to synchronous or asynchronous classroom settings. Facilitated through Learning Management Systems (LMS), many teaching and learning practices took place in web-based environments. Some schools allowed a combination of in-person or hybrid classes by complying with evolving COVID-19 protocols (e.g., use of hand sanitizer, social distancing, facial coverings). These novel implementations raised …

Using The Modern Classrooms Project Instructional Model To Address Post-Covid Challenges In The Classroom, Mary Clare Murray

Department of Teaching, Learning, and Teacher Education: Theses and Other Student Research

The trauma and upheaval that students and families suffered due to the Covid pandemic will impact the classroom in the 2021-2022 school year in two significant ways: in the uncertain social-emotional health of students and in the dramatic disparities in their recent learning experiences. What is the most effective method for teachers to address these issues during instruction? The Modern Classrooms teaching method emphasizes self-paced, mastery-based, blended learning that could be a successful method to address post-Covid challenges in the classroom. This paper refers to studies that show the success of each of these instructional aspects: self-paced, mastery-based, and blended …

Spaces And Societal Interactions: Foundations Of The Critical Disabled Cultural Lens Of A Child Of Disabled Adults, Amelia-Marie Altstadt

Department of Educational Administration: Dissertations, Theses, and Student Research

CoDisA are present on our campuses, but not present within research. This autoethnographic study focuses on providing the foundation of the critical disabled cultural lens of a Child of Disabled Adults (CoDisA) for future study of CoDisA within higher education research. The findings of spaces and societal interactions are presented through the accessible format of autoethnodrama. This two act show is a fun and immersive way to take you on a college tour trip “up the 5," from San Diego, California to Rohnert Park, California in Sonoma County. Act 1, the findings chapter with thorough scene descriptions, helps frame where …

Pre-Milling Interventions For Improving The Microbiological Quality Of Wheat, Shpresa Musa

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Development of pre-milling intervention strategies to improve the microbial quality of wheat and wheat-based products is an emerging concern for the food industry. When steam tempering conditions for hard and soft wheat were applied, the microbial population associated with the grain was significantly reduced compared to controls. Further reductions were observed when acid was applied as part of the intervention which is an added benefit since the addition of a tempering solution is required for both wheat classes since the desired milling moisture cannot be reached by applying steam alone. On average, the highest reductions for hard wheat were achieved …

Spatiotemporal Trends In Bacterial Diversity Across Three Watersheds Within The Platte River Basin, Nebraska, Esther Perisho

School of Natural Resources: Dissertations, Theses, and Student Research

River bacteria are understudied despite being critical components of river ecosystems. There are even fewer studies considering bacteria communities at large spatiotemporal scales, which may provide insight into drivers of community assembly. We investigated differences in bacterial diversity across environmental gradients within three sub-basins nested in the Platte River Basin, Nebraska. Surface water samples were collected weekly at 36 sites from May to September by the Nebraska Department of Environment and Energy (NDEE) in 2019. Bacterial communities were sequenced using the Illumina MiSeq platform. Sub-basins had similar counts of unique amplicon sequence variants (ASVs) but different community structures. These structural …

Characterization Of Western Corn Rootworm (Coleoptera: Chrysomelidae) Susceptibility To Foliar Insecticides In Northeast Nebraska, Timothy B. Dang

Department of Entomology: Dissertations, Theses, and Student Research

The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a major pest of maize in the United States. A variety of tactics are used to manage this pest such as crop rotation, insecticides, and transgenic maize expressing insecticidal proteins from Bacillus thuringiensis (Bt). WCR populations are highly adaptive and have evolved resistance to these management tactics. Management options are limited as few new tactics are available. Research is needed to evaluate the value of existing tactics used within an integrated framework to manage densities/injury and mitigate resistance.

This study evaluated the field performance of formulated foliar insecticides …

Right Side Up: Payment For Ecosystem Services On Privately Owned Grasslands In Nebraska, Kyle Martens

School of Natural Resources: Dissertations, Theses, and Student Research

Grasslands are an important ecological and economic resource in the United States. As part of a natural system, these landscapes can provide income for ranching operations and employment in rural communities; habitat for grassland plants, animals, and migratory species; and offer other services not always readily observed such as improved soil health, clean water, and carbon sequestration. Despite the overarching benefits, the conversion of grasslands to other uses remains widespread throughout much of the remaining Great Plains ecosystem.

Shifting from livestock ranching to another land use often reflects a tipping point. This occurs when the alternative land use is perceived …

African Swine Fever Virus Cd2v Protein Induces Β-Interferon Expression And Apoptosis In Swine Peripheral Blood Mononuclear Cells, Sabal Chaulangain, Gustavo A. Delhon, Sushil Khatiwada, Daniel L. Rock

School of Veterinary and Biomedical Sciences: Faculty Publications

African swine fever (ASF) is a hemorrhagic disease of swine characterized by massive lymphocyte depletion in lymphoid tissues due to the apoptosis of B and T cells, a process likely triggered by factors released or secreted by infected macrophages. ASFV CD2v (EP402R) has been implicated in viral virulence and immunomodulation in vitro; however, its actual function(s) remains unknown. We found that CD2v expression in swine PK15 cells induces NF-κB-dependent IFN-β and ISGs transcription and an antiviral state. Similar results were observed for CD2v protein treated swine PBMCs and macrophages, the major ASFV target cell. Notably, treatment of swine PBMCs and …

Biomaterials With Structural Hierarchy And Controlled 3d Nanotopography Guide Endogenous Bone Regeneration, Shixuan Chen, Hongjun Wang, Valerio Luca Mainardi, Giuseppe Talò, Alec Mccarthy, Johnson V. John, Matthew J. Teusink, Liu Hong, Jingwei Xie

Department of Chemistry: Faculty Publications

Biomaterials without exogenous cells or therapeutic agents often fail to achieve rapid endogenous bone regeneration with high quality. Here, we reported a class of three-dimensional (3D) nanofiber scaffolds with hierarchical structure and controlled alignment for effective endogenous cranial bone regeneration. 3D scaffolds consisting of radially aligned nanofibers guided and promoted the migration of bone marrow stem cells from the surrounding region to the center in vitro. These scaffolds showed the highest new bone volume, surface coverage, and mineral density among the tested groups in vivo. The regenerated bone exhibited a radially aligned fashion, closely recapitulating the scaffold’s architecture. The organic …

Metabolic Modeling And Omics-Integrative Analysis Of Single And Multi-Organism Systems: Discovery And Redesign, Mohammad Mazharul Islam

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Computations and modeling have emerged as indispensable tools that drive the process of understanding, discovery, and redesign of biological systems. With the accelerating pace of genome sequencing and annotation information generation, the development of computational pipelines for the rapid reconstruction of high-quality genome-scale metabolic networks has received significant attention. These models provide a rich tapestry for computational tools to quantitatively assess the metabolic phenotypes for various systems-level studies and to develop engineering interventions at the DNA, RNA, or enzymatic level by careful tuning in the biophysical modeling frameworks. in silico genome-scale metabolic modeling algorithms based on the concept of optimization, …