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Constructing An Attractive Targeted Sugar Bait For Reducing Mosquito Populations, Xi Xian Ng May 2025

Constructing An Attractive Targeted Sugar Bait For Reducing Mosquito Populations, Xi Xian Ng

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Mosquito-borne pathogen transmission continues to pose a significant burden to global public health, well-being, and economic productivity. In the absence of universally available vaccines and amidst rising insecticide resistance that leads to product failures, there is an urgent need to develop new tools to mitigate mosquito-borne disease risk. Two general strategies exist to reduce transmission risk: personal protective measures and area-wide chemical interventions. Attractive Targeted Sugar Bait (ATSB) systems leverage the sugar-feeding behavior of mosquitoes—critical for sustaining their flight, metabolism, development, and fecundity—by delivering oral toxicants through attractively formulated sugar baits. This study aimed to develop an optimized ATSB formulation …


Modeling Degradation In Li-Ion Batteries: Analyzing Single-Cell Degradation And Applying It To Customized Pack-Level, Mohannad Y. Alkhalil May 2025

Modeling Degradation In Li-Ion Batteries: Analyzing Single-Cell Degradation And Applying It To Customized Pack-Level, Mohannad Y. Alkhalil

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

The rapid expansion of lithium-ion (Li-ion) battery applications in areas such as electric vehicles (EVs), renewable energy storage, and portable electronics has drawn attention to the need for improving their performance, safety, and longevity. As Li-ion batteries become essential across technologies, understanding degradation mechanisms is critical for optimizing design and ensuring reliable operation. This work provides a detailed overview of modeling degradation in Li-ion batteries, focusing on single-cell behavior and its implications for pack-level performance.

The PyBaMM (Python Battery Mathematical Modeling) package, an open-source battery simulation tool written in Python, is used for single-cell simulations. Liionpack, another Python-based library, is …


“Re-Storying” Anne Shirley And The Adhd Child: A Crip Genealogy Of Anne Of Green Gables, Darian Aleyna Wilson May 2025

“Re-Storying” Anne Shirley And The Adhd Child: A Crip Genealogy Of Anne Of Green Gables, Darian Aleyna Wilson

Department of English: Dissertations, Theses, and Student Research

This thesis explores the contested nature of Attention Deficit Hyperactivity Disorder (ADHD), focusing on its cultural and historical constructions. While ADHD has been included in the Diagnostic and Statistical Manual of Mental Disorders since 1980, its legitimacy remains a subject of debate across medical, disability studies, and historical scholarship. Using Eli Clare’s critique of diagnosis as a tool rather than a fact, this project examines how ADHD is both pathologized and re-storied within societal and medical discourses. It highlights the tension between medical classifications and lived experiences of those diagnosed with ADHD, particularly in the context of girlhood.

The central …


Stereotypic Behaviors And Physiological Indicators Of Stress Occur In Horses With Restricted Durations Of Turnout, Elizabeth E. Rodgers May 2025

Stereotypic Behaviors And Physiological Indicators Of Stress Occur In Horses With Restricted Durations Of Turnout, Elizabeth E. Rodgers

Department of Animal Science: Dissertations, Theses, and Student Research

The confinement of horses to stalls with limited or no turnout time restricts their natural roaming behaviors, which potentially increases stress and behavioral stereotypies. This study investigated the impact of turnout duration on behavioral and physiological stress indicators in healthy horses. Adult horses (n = 11) were enrolled in a 3 treatment (0-, 15-, and 60-minute turnout/day) Latin square design, including 3 12 d experimental periods over 6 weeks. Horses were acclimated to turnout (24.38 x 64.01m indoor arena) and stalls (2.74 x 4.87m - 3.66 x 3.66m) stalls prior to data collection on day 1. Experienced handlers managed …


Digestibility, Palatability, And Nutrient Composition Of Three Species Of Whole Prey In Three Species Of Snakes, Morgan R. Kienzle May 2025

Digestibility, Palatability, And Nutrient Composition Of Three Species Of Whole Prey In Three Species Of Snakes, Morgan R. Kienzle

Department of Animal Science: Dissertations, Theses, and Student Research

Snakes in human care often lack diversity in the diet. Rodents account for the majority of meals, while wild snakes are consuming a varied diet composed of multiple different whole prey species. By feeding only one species of prey, the chance of nutrient deficiencies and other health concerns increases. To promote variety in the managed diet, two commercially available whole prey species (mice (Mus musculus) and chicks (Gallus spp.)) and one novel species (Seba’s short-tailed bats (Carollia perspicillata)), were analyzed for their dry matter (DM), organic matter (OM), crude protein (CP), crude fat …


Evaluation Of Two Different Sweet Bran Products On Beef Cattle Digestion Characteristics And A Review Of Ramp As An Alternative Step-Up Program, Emily K. Hoefling May 2025

Evaluation Of Two Different Sweet Bran Products On Beef Cattle Digestion Characteristics And A Review Of Ramp As An Alternative Step-Up Program, Emily K. Hoefling

Department of Animal Science: Dissertations, Theses, and Student Research

Two Sweet Bran products were evaluated at 13%, 18%, and 23% inclusion of the diet on total tract digestibility and rumen fermentation parameters. Seven ruminally cannulated, heifers were used in a 6 x 7 row-column design with 21-d periods consisting of 16-d adaptation period followed by a 5-d collection. Dietary treatments were designed in a 2 x 3 factorial arrangement with 6 periods. Factors included two Sweet Bran products that differed in particle size (Sweet Bran-SP; GMD = 1.79 mm or Sweet Bran-LP; GMD = 2.51 mm) and three dietary inclusion (13%, 18%, or 23% of diet dry matter). The …


Methane Mitigation And Its Effects On Energy Utilization In Lactating Dairy Cattle, Shan C. Sherwood May 2025

Methane Mitigation And Its Effects On Energy Utilization In Lactating Dairy Cattle, Shan C. Sherwood

Department of Animal Science: Dissertations, Theses, and Student Research

Improved methane (CH4) mitigation strategies are needed to achieve the greenhouse gas emission goals of the dairy industry. The first experiment was conducted to evaluate the effects of feeding black cumin (Nigella sativa) seed oil and acetate (BCA) on CH4 production and whole-animal energy utilization. Twelve dry Jersey cows were used in a 2-period crossover design study with 28d periods. The BCA treatment was compared to a control diet with corn oil. Both BCA and corn oil were added to the base diet as a top dress. Results from this study did not show any …


Small Cereal Grain Silage Producer Practices And Key Factors To Consider For Operations, Alexa R. Simons May 2025

Small Cereal Grain Silage Producer Practices And Key Factors To Consider For Operations, Alexa R. Simons

Department of Animal Science: Dissertations, Theses, and Student Research

The use of small grain silage to feed livestock has grown in popularity, although the more common source of silage is annual corn. A benefit of producing small cereal grain is double cropping, harvest a forage source in the spring prior to planting the summer annual crop. Although the cereal and corn silage processes are similar there are key differences within management decisions for producers. Objective one was to understand current management decisions from Nebraska producers pre- and post- fermentation surveys along with small cereal silage samples were analyzed. Some best management practices such as the dry matter (DM), DM …


Precision Feeding Methods To Increase Nitrogen And Energy Utilization In Lactating Dairy Cows, Chloe Emily Moss Harris May 2025

Precision Feeding Methods To Increase Nitrogen And Energy Utilization In Lactating Dairy Cows, Chloe Emily Moss Harris

Department of Animal Science: Dissertations, Theses, and Student Research

The environmental and economic costs of overfeeding N to dairy cattle are a challenge in today’s dairy industry. Two studies were conducted to address new opportunities for precision feeding N, which is a strategy where producers strive to feed cows the precise amount of N and amino acids that meet their individual requirements.

The objective of the first study was to evaluate the effects of supplementing a rumen protected methionine (RPM) product via automatic milking system (AMS) concentrate on milk protein yield in lactating dairy cows. Fifty-one cows were randomly assigned to control (no RPM) or treatment groups. Milk yield …


From Activity To Adaptability: Systematic Review On Elevating Adolescent Resilience Through Physical Activity, Kaylee Cowdrey May 2025

From Activity To Adaptability: Systematic Review On Elevating Adolescent Resilience Through Physical Activity, Kaylee Cowdrey

Honors Program: Senior Projects (Public)

The recent increase in mental health concerns among teens showcases the need for accessible solutions that help buffer against psychopathology and build resilience. This systematic literature review looks at various studies to see how physical activity can positively affect resilience in adolescents by enhancing their emotional regulation, self-efficacy, self-esteem, social connections, and brain function. Literature published since 2010 from databases, including APA PsycInfo, PubMed, and Google Scholar, was collected for this analysis. The results of the review found that as adolescents engage in physical activity, there is improvement in coping skills by enhancing emotional regulation, self-efficacy, self-esteem, and social connectedness …


Caterpillar Strategic Audit, Tyler Vander Woude, Grant Kingston, Kaiden Bedient, Drew Shafer May 2025

Caterpillar Strategic Audit, Tyler Vander Woude, Grant Kingston, Kaiden Bedient, Drew Shafer

Honors Program: Senior Projects (Public)

This strategic audit evaluates Caterpillar Inc.'s business-level and corporate-level strategies, examining how the company maintains its competitive advantage in the heavy machinery and industrial equipment sectors. The research focuses on Caterpillar's hybrid approach, combining differentiation and cost leadership strategies to offer high-quality, durable products while optimizing costs through technological innovation, supply chain efficiencies, and product longevity. The methodology for this audit includes a comprehensive internal and external analysis, incorporating frameworks such as SWOT, PESTEL, VRIO, and Porter’s Five Forces. Additionally, Caterpillar’s strategic initiatives in vertical and horizontal integration, governance structure, and financial performance were assessed. The research also explores the …


Beyond The Label Campaign: An Integrated Marketing And Communications Campaign To Educate And Empower Low-Income Communities In The Southern United States, Erica Napuli May 2025

Beyond The Label Campaign: An Integrated Marketing And Communications Campaign To Educate And Empower Low-Income Communities In The Southern United States, Erica Napuli

Honors Program: Senior Projects (Public)

The Beyond the Label campaign is an Integrated Marketing Communications (IMC) initiative designed to educate and empower low-income consumers about food additives, labeling regulations and the impact of making educated food choices on long-term health. This campaign is based on research on current U.S. food labeling and regulations, health risks associated with current ingredients and additives approved through regulations, the disproportionate impact of food policy on low-income communities with a focus on the Southern U.S., as well as further exploration of strategies for multi-channel marketing for targeted audiences. This project proposes a hypothetical partnership with the Environmental Working Group (EWG). …


Nebraska's Preparedness For A Growing Diverse Aging Population: A Survey Of Area Agencies On Aging, Vince Barger May 2025

Nebraska's Preparedness For A Growing Diverse Aging Population: A Survey Of Area Agencies On Aging, Vince Barger

Honors Program: Senior Projects (Public)

This paper aims to examine the current efforts of Nebraska’s Area Agencies on Aging (AAAs) to serve a growing diverse population of older adults (United States Census Bureau, 2023). This project is comprised of a survey created to gauge the thoughts and opinions of Nebraska AAA employees supplemented by a comprehensive literature review. The findings of the literature review indicate the need for an understanding of the importance of diversity and how it affects individual life experiences. The survey revealed that the AAAs are already making changes to reflect the population shift that is currently happening, such as specific programs …


Evolution And Strengths Of School Lunch Programs In The United States And Japan, Sarah Ritterling May 2025

Evolution And Strengths Of School Lunch Programs In The United States And Japan, Sarah Ritterling

Honors Program: Senior Projects (Public)

School lunches around the world perform a vital role in children’s nutritional needs and overall health. These nutritional programs provide a chance for children to learn how to create a balanced meal and about the production and serving of food. However, many countries, including the United States and Japan, have taken different approaches to meet these goals, influenced by the needs of children and communities over time. The United States provides support, funding, and nutritional standards through the National School Lunch Program. Japan promotes the Basic Act of Shokuiku (food and nutrition education) within society and outlines goals for schools …


Sisterly Bonds Beyond The Grave: The Ethical Implications Of Posthumously Publishing Jane Austen’S Persuasion, Ashleigh Jay May 2025

Sisterly Bonds Beyond The Grave: The Ethical Implications Of Posthumously Publishing Jane Austen’S Persuasion, Ashleigh Jay

Honors Program: Senior Projects (Public)

By the time that Jane Austen died in 1817, she had only published four novels. After her death, her family retained her other writings. Later that year, they published one of Austen’s finished manuscripts which became the novel Persuasion. This act of posthumous publication invokes inquiries into whether the action was ethical. In order to better understand the ethics of publishing Persuasion posthumously, one can look at information from Jane Austen’s life and ideas around posthumous actions. Even though Persuasion by Jane Austen was published posthumously, Austen’s choice to leave the manuscript with her sister and her completion of …


Strategic Audit: Hilton Worldwide Holdings, Inc., Jenna Derowitsch, Tram Ngo, Tenley Katt, Makenna Henning, Lexi Soukup May 2025

Strategic Audit: Hilton Worldwide Holdings, Inc., Jenna Derowitsch, Tram Ngo, Tenley Katt, Makenna Henning, Lexi Soukup

Honors Program: Senior Projects (Public)

This comprehensive strategic audit analyzes the internal and external environment in which Hilton Worldwide Holdings Inc. operates, in addition to auditing the strategic drivers that have drove the iconic hospitality firm to success. Hilton Worldwide Holdings, Inc. (Hilton) is a valued hotel chain that has been operating as a global leader in the hospitality industry for more than 100 years. The firm sets an industry standard to provide high-quality hotel services and a reliable stay for more than 200 million guests annually and continues to develop its portfolio of destinations. Technological advancements and shifting customer preferences challenge Hilton to remain …


Dutch Bros Coffee Strategic Audit, Parker Barry, Olivia Palmer, Cade Swanson, Braden Starck, Jacob Whiston May 2025

Dutch Bros Coffee Strategic Audit, Parker Barry, Olivia Palmer, Cade Swanson, Braden Starck, Jacob Whiston

Honors Program: Senior Projects (Public)

This case study focuses on Dutch Bros, a leading drive-thru coffee chain. A strategic audit was conducted to understand the company’s strategic management principles: external and internal factors, performance and competition, business level strategy, corporate level strategy, and organizational design and governance. In addition, the audit studies the implications of their recent strategic decisions to expand roasting facilities and move towards corporate-owned stores rather than operating a franchise model.

The research contained in this audit used publicly available information, including Dutch Bro’s financial statements, information from their website, annual reports, IBIS industry reports, and online press coverage.


Intersectionality In Tennis (Wta) Sports Media, Emily Stoner May 2025

Intersectionality In Tennis (Wta) Sports Media, Emily Stoner

Honors Program: Senior Projects (Public)

This “applied knowledge” project investigates and observes intersectionality in tennis sports media via the women’s professional tour (WTA). Intersectionality in Tennis (WTA) Sports Media analyzes how intersectionality as a concept and social justice lens impacts and is impacted by media. By considering intersectional identities and their implications in sports media narratives and coverage, this piece displays some telling patterns and perpetuations.

At the professional level, tennis certainly does not garner the most profit or attention, but it provides a unique world of intersecting identities, cultures, geography and tradition. This work aims to shed light on the state of sports media, …


Women Composers’ Music Contributions Equal To Those Of Male Counterparts During The Romantic Era, Chelsea J. Abels May 2025

Women Composers’ Music Contributions Equal To Those Of Male Counterparts During The Romantic Era, Chelsea J. Abels

Honors Program: Senior Projects (Public)

Though women composers were downplayed and overlooked, they were no less talented than their male counterparts, providing meaningful compositions and contributions to Romantic Era music. This thesis studies three women who made meaningful contributions during the Romantic period. Their names are Clara Schumann, Amy Beach, and Fanny Hensel Mendelssohn. Four aspects about them will be studied: The life and childhood of each woman, the trials and discrimination they faced, the problems they solved, and how their works compare to male composers. These three women were prodigy musicians and skilled composers. They demonstrated a great affinity for music from an early …


The Digital Glow: Effects Of Blue Light On Children And The Development Of A Children's Book To Raise Awareness On Eye Health, Mallory Easland May 2025

The Digital Glow: Effects Of Blue Light On Children And The Development Of A Children's Book To Raise Awareness On Eye Health, Mallory Easland

Honors Program: Senior Projects (Public)

As digital screen use continues to rise among children, concerns about the effects of blue light on eye health, sleep patterns, and overall well-being have grown. Research suggests that prolonged exposure to blue light can contribute to digital eye strain, disrupt circadian rhythms, and impact cognitive development. This thesis explores scientific literature on the biological effects of blue light, specifically on children, examining both the risks associated with excessive exposure and strategies for mitigating its impact.

In addition to conducting a comprehensive literature review, this project applies research findings to the creation of an educational children’s book aimed at promoting …


Gut Feeling: A Holistic Digital Tool For Managing Irritable Bowel Syndrome And Food Sensitivities, Hailey M. Olberding May 2025

Gut Feeling: A Holistic Digital Tool For Managing Irritable Bowel Syndrome And Food Sensitivities, Hailey M. Olberding

Honors Program: Senior Projects (Public)

Irritable Bowel Syndrome (IBS) and food intolerances are increasingly prevalent chronic gastrointestinal (GI) conditions that significantly impair quality of life. While the low-Fermentable Oligosaccharides, Disaccharides, Monosaccharides and Polyols (FODMAP) diet has demonstrated success in symptom management, current digital tools fail to offer integrated, user-friendly platforms that holistically track contributing health factors. Gut Feeling is a mobile app (Appendix 1) and complementary website (Appendix 2) developed to fill this gap. By incorporating symptom logging, food intake tracking and complementary database, stress and sleep monitoring, and trend analysis, this platform empowers users to better understand their bodies and manage GI symptoms proactively. …


Entre La Reforma Y La Realidad: Salud Y Derechos Humanos En Costa Rica, Cuba Y Uruguay, Alyssa M. Nielsen May 2025

Entre La Reforma Y La Realidad: Salud Y Derechos Humanos En Costa Rica, Cuba Y Uruguay, Alyssa M. Nielsen

Honors Program: Senior Projects (Public)

Latinoamérica tiene varios sistemas para el cuidado de la salud y el acceso es uno de la lista de los derechos humanos. Esta región es la más desigual del mundo y se han logrado grandes avances, pero no se han distribuido equitativamente. Los sistemas de Cuba, Costa Rica y Uruguay son bien desarrollados, pero funcionan diferente. Costa Rica y Uruguay tienen opciones públicas y privadas y tienen maneras como la Mutualista en Uruguay y la Caja en Costa Rica para cubrir las visitas y servicios necesarios. Cuba es el sistema más controversial y tiene gran influencia de Castro, de donde …


Conagra Brands Strategic Audit, Ashley R. Nierman May 2025

Conagra Brands Strategic Audit, Ashley R. Nierman

Honors Program: Senior Projects (Public)

The goal of this paper is to present a comprehensive strategic audit of Conagra Brands, Inc., a major company in the consumer-packaged foods industry. The audit evaluates the external environment of Conagra, including a PESTEL analysis and Porter’s Five Forces analysis. Various industry factors that affect Conagra’s operations are taken into consideration. It will also explain the internal environment of Conagra using tools such as a SWOT analysis, financial performance review, and strategy explanation. Key areas of focus include shifting demands, including consumers’ desire for more healthy and sustainable food options, recent political and economic factors, competitive advantages of Conagra, …


A Strategic Audit: Eli Lilly & Company, Natalie Karrels, Ethan Heinemann, Jonathan Patton, Meg Williams May 2025

A Strategic Audit: Eli Lilly & Company, Natalie Karrels, Ethan Heinemann, Jonathan Patton, Meg Williams

Honors Program: Senior Projects (Public)

This strategic audit assesses the external and internal forces impacting Eli Lilly & Company’s strategic decision-making strategies. All information has been gathered from industry reports and company reports, including reports for investors, analyst calls, financial statements, and news reports. The report highlights the quantitative, qualitative, performance, strategy, and leadership decisions that have allowed Eli Lilly & Company to succeed for the past 150 years.


Assessment Of Energy Efficiency Measures Recommended For Industrial Facilities: Environmental, Economic, And Life Cycle Perspectives, Dariush Jafari May 2025

Assessment Of Energy Efficiency Measures Recommended For Industrial Facilities: Environmental, Economic, And Life Cycle Perspectives, Dariush Jafari

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

This study assesses the economic, environmental, and life cycle impacts of more than 800 industrial Energy Efficiency Measures (EEMs) identified in manufacturing facilities across Nebraska. These EEMs are notable for their rapid implementation and minimal disruption to the facility’s building structure or core processes.

The research evaluates the financial viability of EEMs by analyzing utility savings, implementation costs, and payback periods. It also examines their cost-effectiveness in reducing greenhouse gas (GHG) emissions, including calculations of net cost and cost of mitigated CO₂-eq.

Key findings indicate that adding insulation to hot pipes and buildings, replacing personal fans with High-Volume, Low-Speed (HVLS) …


A Framework For Quantifying The Benefits Of Electric Vehicle Infrastructure Development, Donya Negahbani May 2025

A Framework For Quantifying The Benefits Of Electric Vehicle Infrastructure Development, Donya Negahbani

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Under the Biden Administration’s Justice40 Initiative, a significant portion of benefits derived from federal clean transportation investments is required to be directed to disadvantaged communities. Meeting this mandate necessitates a transparent and reproducible approach that quantifies various dimensions of value and allocates them equitably. This dissertation presents a flexible framework specifically tailored for the deployment of electric vehicle charging infrastructure in Nebraska, effectively translating equity goals into rigorous spatial and economic analyses. The framework first identifies multiple distinct categories of benefit that reflect the societal value of charging access: energy savings, economic opportunities, environmental quality enhancements, and public health improvements. …


Addressing Structural Violence In Eastern Nebraska Through The Assessment Of Mortality Profiles, Victoria M. Pate May 2025

Addressing Structural Violence In Eastern Nebraska Through The Assessment Of Mortality Profiles, Victoria M. Pate

Department of Anthropology: Theses and Student Research

Humanitarian forensics has recently expanded to include the examination of forensic casework demographics within the medicolegal community. Additionally, it is proposed that research and data collection should look beyond the individual case report to address public health, crime vulnerabilities, and marginalization at local and national levels to advocate for policy changes protecting against institutionalized violence. With the utilization of quantitative and qualitative methods, 294 case reports provided by Nebraska police departments, between the years of 2014-2024, were analyzed to conceptualize mortality vulnerabilities and the ways in which forensic anthropologists can aid in community action. Pearson’s Chi-Square and one-way Analysis of …


The Perry-Campbell Collection: An Analysis Of Byzantine And Roman Coins, Samuel A. Skokan May 2025

The Perry-Campbell Collection: An Analysis Of Byzantine And Roman Coins, Samuel A. Skokan

Department of Anthropology: Theses and Student Research

The Anthropology Collections of the Nebraska State Museum are home of the Gladys G. Perry Campbell and Iain Colin Gordon Campbell (I. C. G. Campbell) coin collection, which consists of an estimated 4,000 coins that date between the 1st century BC to the 15th century AD. The collection consists of bronze coins (91%+) and billon (alloy) coins that represent low-denomination currency of the Roman and Byzantine Empires. There are approximately 3,200 Byzantine and 750 Roman coins. This study focused solely on the Byzantine coins of the Collection, to document their chronological range and research value. The collection can support …


Internalized Homophobia In Midwestern Sexual Minorities, Charissa Farr May 2025

Internalized Homophobia In Midwestern Sexual Minorities, Charissa Farr

Department of Anthropology: Theses and Student Research

Research into internalized homophobia in sexual minorities has identified several factors that tend to lead to higher levels of internalized homophobia. These factors are parental acceptance or rejection, religion, urban versus rural environment, and societal attitudes. There are, however, few studies that have been done on these factors specifically in Midwestern sexual minorities. Twenty participants that identified as non-heterosexual were interviewed for this study. Participants completed a short quantitative survey on internalized homophobia before being interviewed with questions that looked at the known factors of internalized homophobia as well as what they thought needed to happen for future generations to …


Host Genetics Role In Viral Disease Susceptibility, Christian Willis Eaton May 2025

Host Genetics Role In Viral Disease Susceptibility, Christian Willis Eaton

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Viruses and their hosts exist in an evolutionary arms race, with evolutionary promotion of host genetic-based defense mechanisms and evolution of virus genetic-based mechanisms to overcome host defenses. Viruses are capable of much more rapid evolution and adaptation than their hosts, often leading to the use of host cellular mechanisms to promote viral infection and replication. In this study, we describe the generation of a detection assay for atypical porcine pestivirus (APPV), designed to target the viral 5’ UTR, a region of the viral genome which sees less mutations than the protein coding regions. This assay was generated to be …