Digital Commons Network^™

Fungi: Friend Or Foe? A Mycobiome Evaluation In Children With Autism And Gastrointestinal Symptoms, Jane Alookaran, Yuying Liu, Thomas Auchtung, Amirali Tahanan, Manouchehr Hessabi, Parisa Asgarisabet, Mohammad H. Rahbar, Nicole Y. Fatheree, Deborah A. Pearson, Rosleen Mansour, Melissa R. Van Arsdall, Fernando Navarro, J. Marc Rhoads

Department of Food Science and Technology: Faculty Publications

Gastrointestinal (GI) symptoms often affect children with autism spectrum disorders (ASD) and GI symptoms have been associated with an abnormal fecal microbiome. There is limited evidence of Candida species being more prevalent in children with ASD. We enrolled 20 children with ASD and GI symptoms (ASD + GI), 10 children with ASD but no GI symptoms (ASD - GI), and 20 from typically developing (TD) children in this pilot study. Fecal mycobiome taxa were analyzed by Internal Transcribed Spacer sequencing. GI symptoms (GI Severity Index [GSI]), behavioral symptoms (Social Responsiveness Scale -2 [SRS-2]), inflammation and fungal immunity (fecal calprotectin and …

Seventh Grade Book Club Unit, Emily Donnell

Honors Program: Senior Projects (Public)

The purpose of this book club unit is to teach students the valuable lessons they need to learn about adversity and emotions while still preparing them for the assessments that are required by school districts. This unit is centered around “Drums Girls and Dangerous Pie” and “Wonder”. These books are part of the required curriculum for on-level 7th graders within Lincoln Public Schools (LPS) in Lincoln, NE. Open discussions and collaboration are the two main ways that students will come to understandings within this unit.

Furthering Women In Policing: How A Police Department's Duty Firearm Selection Process May Mitigate The Gender Disparity In Marksmanship, Jenna Prochnau

Honors Program: Senior Projects (Public)

Previous research has revealed that there are several benefits to increasing the representation of women in law enforcement, including less use of force, increased community trust, and better outcomes for domestic violence and sexual assault victims. While many police departments now recognize these benefits and are aiming to recruit more women, a problem persists because women are less likely to graduate from police academies than their male counterparts. A significant difference has been observed in the area of marksmanship, particularly, with more female recruits failing to meet firearm scoring standards. Currently, very little is known about how police departments select …

Adaptation, Transformation And Resilience In Healthcare Comment On “Government Actions And Their Relation To Resilience In Healthcare During The Covid-19 Pandemic In New South Wales, Australia And Ontario, Canada”, David G. Angeler, Harris A. Eyre, Michael Berk, Craig R. Allen, William Hynes, Igor Linkov

School of Natural Resources: Faculty Publications

Adaptive capacity is a critical component of building resilience in healthcare (RiH). Adaptive capacity comprises the ability of a system to cope with and adapt to disturbances. However, “shocks,” such as the current coronavirus disease 2019 (COVID-19) pandemic, can potentially exceed critical adaptation thresholds and lead to systemic collapse. To effectively manage healthcare systems during periods of crises, both adaptive and transformative changes are necessary. This commentary discusses adaptation and transformation as two complementary, integral components of resilience and applies them to healthcare. We treat resilience as an emergent property of complex systems that accounts for multiple, often disparately distinct …

Uncovering The Potential Learning In Short-Term Study Abroad, Elizabeth Niehaus, Gudrun Nyunt

Department of Educational Administration: Faculty Publications

The purpose of this quantitative study was to identify the facets of intercultural learning that might be achievable through faculty-led, short-term study abroad. Exploring changes in various facets of students’ intercultural competence pre- to post-study abroad, we found that the largest gains were in the cultural knowledge domain. Findings also point to vast differences in how individual students do, and do not, show gains across multiple measures of intercultural competence after studying abroad.

Challenges In Gluten Analysis: A Comparison Of Four Commercial Sandwich Elisa Kits, Plaimein Amnuaycheewa, Lynn Niemann, Richard E. Goodman, Joseph L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Gluten is composed of prolamin and glutelin proteins from several related grains. Because these proteins are not present in identical ratios in the various grains and because they have some differences in sequence, the ability to accurately quantify the overall amount of gluten in various food matrices to support gluten-free labeling is difficult. Four sandwich ELISAs (the R-Biopharm AG R5 RIDASCREEN®, the Neogen Veratox® R5, the Romer Labs AgraQuant® G12, and the Morinaga Wheat kits) were evaluated for their performance to quantify gluten concentrations in various foods and ingredients. The Morinaga and AgraQuant® G12 tests yielded results comparable to the …

Enhancing The Bioaccessibility Of Lycopene From Tomato Processing Byproducts Via Supercritical Carbon Dioxide Extraction, Ali Ubeyitogullari, Ozan N. Ciftci

Department of Food Science and Technology: Faculty Publications

Tomato peel and seed from tomato processing industry are treated as waste; however, they contain lycopene, a high-value bioactive compound. In this study, lycopene was extracted from tomato peel and seed using supercritical carbon dioxide (SC–CO₂) and hexane, and the bioaccessibilities of lycopene in the SC-CO₂- and hexaneextracted oleoresins were investigated for the first time. The (Z)-lycopene content of the SC-CO₂-extracted oleoresin (69%) was higher than that of hexane-extracted oleoresin (45%). Separation of the insoluble fraction from the oleoresins increased the (Z)-lycopene contents of the SC-CO₂- and hexane-extracted oil fractions to 80% …

In Memoriam: Robert E. Woodruff, July 20, 1933–July 8, 2021, Paul E. Skelley, Oliver Keller

Insecta Mundi

This and the following set of papers published in Insecta Mundi are dedicated to Robert E. Woodruff, as tribute and thanks from those he mentored or assisted throughout his life. Bob’s many contributions to the study of beetles, being a founding member and first president of the Center for Systematic Entomology (CSE), co-creating Insecta Mundi with Ross H. Arnett, Jr., and having served in various capacities for 30 years on the CSE board of directors and the editorial board of Insecta Mundi, were deemed significant enough to allow this paper outside our normal guidelines. The following pages highlight the life …

Extinct Or Extant? A New Species Of Termitodius Wasmann, 1894, (Coleoptera: Scarabaeidae: Aphodiinae: Rhyparini) With A Short Review Of The Genus, Paul E. Skelley, Julian Clavijo-Bustos, Oliver Keller

Insecta Mundi

A new species of Termitodius Wasmann (Coleoptera: Scarabaeidae: Aphodiinae: Rhyparini) is de­scribed from Colombia, Termitodius woodruffi Skelley, Clavijo-Bustos, and Keller, new species. This species is both extant and abundantly preserved in copal. The genus Termitodius is reviewed with a key and brief accounts to all species.

Una nueva especie de Termitodius Wasmann (Coleoptera: Scarabaeidae: Aphodiinae: Rhyparini) es descrita de Colombia, Termitodius woodruffi Skelley, Clavijo-Bustos, y Keller, nueva especie. Esta especie es existente y abundantemente preservada en copal. El género Termitodius es revisado con una clave y reseñas breves para todas las especies.

The pantropical tribe Rhyparini is represented …

Ecology And Diversity Of Culturable Fungal Species Associated With Soybean Seedling Diseases In The Midwestern United States, Mirian F. Pimentel, Ali Y. Srour, Amanda J. Warner, Jason P. Bond, Carl A. Bradley, John Rupe, Martin I. Chilvers, J. Alejandro Rojas, Janette L. Jacobs, Christopher R. Little, Alison E. Robertson, Loren J. Giesler, Dean Malvick, Kiersten Wise, Albert Tenuta, Ahmad M. Fakhoury

Department of Plant Pathology: Faculty Publications

Aims: To isolate and characterize fungi associated with diseased soybean seedlings in Midwestern soybean production fields and to determine the influence of environmental and edaphic factors on their incidence.

Methods and Results: Seedlings were collected from fields with seedling disease history in 2012 and 2013 for fungal isolation. Environmental and edaphic data associated with each field was collected. 3036 fungal isolates were obtained and assigned to 76 species. The most abundant genera recovered were Fusarium (73%) and Trichoderma (11.2%). Other genera included Mortierella, Clonostachys, Rhizoctonia, Alternaria, Mucor, Phoma, Macrophomina and Phomopsis. …

Vaccinia Virus Arrests And Shifts The Cell Cycle, Caroline K. Martin, Jerzy Samolej, Annabel T. Olson, Cosetta Bertoli, Matthew S. Wiebe, Robertus A. M. De Bruin, Jason Mercer

School of Veterinary and Biomedical Sciences: Faculty Publications

Modulation of the host cell cycle is a common strategy used by viruses to create a proreplicative environment. To facilitate viral genome replication, vaccinia virus (VACV) has been reported to alter cell cycle regulation and trigger the host cell DNA damage response. However, the cellular factors and viral effectors that mediate these changes remain unknown. Here, we set out to investigate the effect of VACV infection on cell proliferation and host cell cycle progression. Using a subset of VACV mutants, we characterise the stage of infection required for inhibition of cell proliferation and define the viral effectors required to dysregulate …

Tissue Losses And Metabolic Adaptations Both Contribute To The Reduction In Resting Metabolic Rate Following Weight Loss, Alexandra Martin, Darius Fox, Chaise A. Murphy, Hande Hofmann, Karsten Koehler

Department of Nutrition and Health Sciences: Faculty Publications

OBJECTIVE: To characterize the contributions of the loss of energy-expending tissues and metabolic adaptations to the reduction in resting metabolic rate (RMR) following weight loss.

METHODS: A secondary analysis was conducted on data from the Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy study. Changes in RMR, body composition, and metabolic hormones were examined over 12 months of calorie restriction in 109 individuals. The contribution of tissue losses to the decline in RMR was determined by weighing changes in the size of energy-expending tissues and organs (skeletal muscle, adipose tissue, bone, brain, inner organs, residual mass) …

A Review Of The Impacts Of Invasive Wild Pigs On Native Vertebrates, Matthew T. Mcdonough, Stephen S. Ditchkoff, Mark D. Smith, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

The wild pig (Sus scrofa) is a successful invasive species that has become well established outside of its native range in Eurasia. The invasive wild pig is the result of released or escaped domesticated livestock becoming feral, or Eurasian boar introduced for hunting purposes. The global spread of wild pigs has recently been exacerbated in some areas, such as the USA, by anthropogenically assisted dispersal. Once established in novel ecosystems, wild pigs have the potential to have significant negative impacts on the ecosystem, and the scientific literature is replete with examples. It is generally accepted that wild pigs …

Mobility Of Phosphine-Susceptible And -Resistant Rhyzopertha Dominica (Coleoptera: Bostrichidae) And Tribolium Castaneum (Coleoptera: Tenebrionidae) After Exposure To Controlled Release Materials With Existing And Novel Active Ingredients, Sabita Ranabhat, Kun Yan Zhu, Georgina Bingham, William R., Morrison Iii

Department of Entomology: Faculty Publications

There is interest in developing controlled release materials (CRMs) with novel modes of action to improve resistance management. Long-lasting insecticide-incorporated netting (LLIN) with deltamethrin has been effectively used against stored-product pests. Here, we evaluated the efficacy of different CRMs (LLIN or packaging) with each of four active ingredients (AI) (deltamethrin, permethrin, indoxacarb, and dinotefuran) and compared them to control CRMs in reducing movement and increasing mortality of phosphine-susceptible and -resistant Rhyzopertha dominica and Tribolium castaneum. Adults were exposed for 0.5, 2, or 60 min, and movement was assessed immediately or after 24, or 168 h using video-tracking and Ethovision …

Role Of Specialized Pro-Resolving Mediators In Reducing Neuroinflammation In Neurodegenerative Disorders, Jana Ponce, Arzu Ulu, Corrine Hanson, Erin Cameron-Smith, Jenna Wuebker, John Bertoni, Alfred Fisher, Ka-Chun Siu, Vivien Marmelat, Jiri Adamec, Danish Bhatti

Department of Biochemistry: Faculty Publications

Alzheimer’s disease (AD) and Parkinson’s disease (PD) are neurodegenerative disorders that affect millions of individuals worldwide. As incidence of these conditions increases with age, there will undoubtedly be an increased prevalence of cases in the near future. Neuroinflammation is a hallmark in the development and progression of neurodegenerative diseases and prevention or resolution of chronic neuroinflammation may represent a novel approach to treatment. The present review highlights the potential of the anti-inflammatory and pro-resolving effects of polyunsaturated fatty acid (PUFA)- derived mediators (Specialized Pro-resolving Mediators—SPM) in neurodegenerative disorders. PUFA-derived SPM are biosynthesized in response to chemicals produced from acute inflammatory …

Improving Taxonomic Delimitation Of Fungal Species In The Age Of Genomics And Phenomics, Asjley Stengel, Kimberly M. Stanke, Amanda C. Quattrone, Joshua Herr

Department of Plant Pathology: Faculty Publications

Species concepts have long provided a source of debate among biologists. These lively debates have been important for reaching consensus on how to communicate across scientific disciplines and for advancing innovative strategies to study evolution, population biology, ecology, natural history, and disease epidemiology. Species concepts are also important for evaluating variability and diversity among communities, understanding biogeographical distributions, and identifying causal agents of disease across animal and plant hosts. While there have been many attempts to address the concept of species in the fungi, there are several concepts that have made taxonomic delimitation especially challenging. In this review we discuss …

Use Of A Direct, Rapid Immunohistochemical Test For Diagnosis Of Rabies Virus In Bats, Charles E. Rupprecht, Lolita Van Pelt, April D. Davis, Richard B. Chipman, David L. Bergman

United States Department of Agriculture Wildlife Services: Staff Publications

Rabies, a zoonotic encephalitis due to transmission of a lyssavirus, such as rabies virus (RABV), has the highest case fatality of any infectious disease. A global program for the elimination of human rabies caused by dogs is proposed for realization by 2030. Sensitive, specific, and inexpensive diagnostic tests are necessary for enhanced surveillance to detect infection, inform public health and veterinary professionals during risk assessments of exposure, and support overall programmatic goals. Multiple laboratory techniques are used to confirm a suspect case of rabies. One method for the detection of lyssavirus antigens within the brain is the direct rapid immunohistochemical …

Hearing And Vestibular Loss With Misuse Of Opioids And Illicit Drugs: A Review Of The Literature, Michelle L. Hughes, Amanda Rodriquez, Jonathan Hatch, Kenneth Zoucha

Department of Special Education and Communication Disorders: Faculty Publications

Background: The purpose of this review was to summarize the literature regarding the effects of opioids and illicit drugs on the auditory and vestibular systems.

Methods: Data were sourced from published papers reporting hearing loss (HL) and/or vestibular loss (VL) following misuse or overdose of opioids or illicit drugs. Most papers consisted of retrospective single-case reports, with few retrospective reviews or prospective cohort studies. Search terms included variations of HL, VL, opioids, and illicit drugs. Search results yielded 51 articles published between 1976 and 2021. A total of 44 articles were reviewed after excluding studies that were not available in …

Troubling Unintended Harm Of Heroic Discourses In Social Justice Leadership, Taeyeon Kim, Courtney Mauldin

Department of Educational Administration: Faculty Publications

We aim to problematize the ways in which school leaders who seek social justice conflate heroic leadership discourses in their practices. Using qualitative data collected from an urban school principal, this study examines heroic metaphors utilized by the principal when describing social justice leadership and how heroic-centered approaches contradict with achieving social justice goals in school. The findings suggest that the principal’s idea of social justice leadership relies on discourse around “battles to win”, a savior complex, and seeing herself as the central model for driving change. Such heroic discourses reflect the principal’s sole reliance on herself as a savior …

Biogeosciences Perspectives On Integrated, Coordinated, Open, Networked (Icon) Science, D. Dwivedi, A. L. D. Santos, M. A. Barnard, T. M. Crimmins, A. Malhotra, K. A. Rod, K. S. Aho, S. M. Bell, B. Bomfim, F. Q. Brearley, H. Cadillo-Quiroz, J. Chen, C. M. Gough, E. B. Graham, C. R. Hakkenberg, L. Haygood, G. Koren, E. A. Lilleskov, L. K. Meredith, S. Naeher, Z. L. Nickerson, O. Pourret, H.-S. Song, M. Stahl, N. Taş, R. Vargas, S. Weintraub-Leff

Department of Food Science and Technology: Faculty Publications

This article is composed of three independent commentaries about the state of Integrated, Coordinated, Open, Networked (ICON) principles in the American Geophysical Union Biogeosciences section, and discussion on the opportunities and challenges of adopting them. Each commentary focuses on a different topic: (a) Global collaboration, technology transfer, and application (Section 2), (b) Community engagement, community science, education, and stakeholder involvement (Section 3), and (c) Field, experimental, remote sensing, and real-time data research and application (Section 4). We discuss needs and strategies for implementing ICON and outline short- and long-term goals. The inclusion of global data and international …

Negotiating Incomplete Autonomy: Portraits From Three School Principals, Taeyeon Kim, Jennie Weiner

Department of Educational Administration: Faculty Publications

Purpose: This study builds on research scrutinizing school autonomy in policy and school governance by shifting the focus from a formal structural view of autonomy to examining how principals negotiate autonomy in their daily work. Drawing on multiple dimensions of autonomy and street-level bureaucracy, this study examined how principals, as both professionals and bureaucrats, work to expand and strategize their autonomy in practice.

Research Methods/Approach: We used portraiture to document and interpret the experience and perspectives of three principals at urban, suburban, and rural PK-12 traditional public schools in the Midwest of US during the 2018–2019 school year.

Findings: Principals …

The Astounding World Of Glycans From Giant Viruses, Anna Notaro, Chantal Abergel, Rosa Lanzetta, Todd L. Lowary, Antonio Molinaro, Michela Tonetti, James L. Van Etten, Cristina De Castro

Department of Plant Pathology: Faculty Publications

Viruses are a heterogeneous ensemble of entities, all sharing the need for a suitable host to replicate. They are extremely diverse, varying in morphology, size, nature, and complexity of their genomic content. Typically, viruses use host-encoded glycosyltransferases and glycosidases to add and remove sugar residues from their glycoproteins. Thus, the structure of the glycans on the viral proteins have, to date, typically been considered to mimick those of the host. However, the more recently discovered large and giant viruses differ from this paradigm. At least some of these viruses code for an (almost) autonomous glycosylation pathway. These viral genes include …

What Kigali’S Open‑Air Markets Reveal About Achieving Food And Nutrition Security: The Role Of African Indigenous Crops, Eugene Baraka, Mary S. Willis, Brice A. Ishimwe

Department of Nutrition and Health Sciences: Faculty Publications

Background: Household dietary diversity in Rwanda remains low and significantly contributes to the double burden of malnutrition. Rwanda has one of the highest under five stunting rates globally, and malnutrition remains one of the most pressing public health issues; therefore, factors that shape food and nutrition security are of utmost concern. Globally, the variety of foods available in open-air markets has been shown to affect dietary diversity. Furthermore, the consumption of indigenous foods can contribute to a diverse diet and improve nutrition status. At present, there are limited data on foods available for purchase in open-air markets in Africa. …

The Crucial Role Of Sestrins In Oxidative Stress Response And Disease: An Analysis Of Phosphorylation Mutation S110a In Hsesn2, Julianne Fay

Honors Program: Senior Projects (Public)

Sestrins are highly conserved proteins which are induced under environmentally stressful conditions to adjust the cell’s response to such disturbances. This protein possesses two domains critical for its dual function in reducing reactive oxygen species (ROS) and regulating the mTORC1 complex. Sestrins may use diverse transcription factors, such as Nrf2, peroxiredoxin reductase activity, and a catalytic cysteine to regenerate oxidized species; however this was confirmed to be through a mechanism likely different than AhpD, for which there was distance sequence similarity. Furthermore, Sestrins are important regulators of TORC1 and AMPK, which are crucial energy sensors able to control cellular metabolism …

Ahp Based Multi Criteria Decision Analysis Of Success Factors To Enhance Decision Making In Infrastructure Construction Projects, Solomon Belay, James D. Goedert, Asregedew Woldesenbet, Saeed Rokooei

Durham School of Architectural Engineering and Construction: Faculty Publications

The study presents analytical data-based multi-criteria approach of critical success factors of infrastructure construction projects analyzed in the Ethiopian construction industry. This multi-criteria technique helps to improve the decision capabilities and ultimate performance of construction processes in various low-income countries of the East African region. The aim of this paper is to establish a logical relationship and interdependencies of success-related factors for enhancing decision making for various project teams and identify priorities while taking into account all known construction organizational constraints. A structured hierarchical matrix was developed based on a pre-identified success-related factors, and initially evaluated by experienced professionals as …

Trainee Reactions, Megan Paul

Umbrella Summaries

What are trainee reactions?

Trainee reactions refer to “subjective evaluations learners make about their training experiences” (Sitzmann et al., 2008, p. 280). Commonly referred to as “level 1” evaluations (e.g., Kirkpatrick, 1998), reactions are the most commonly used type of training evaluation and are measured with post-training surveys. Though measures are occasionally included in published research, there are no universal or even widely used measures. Instead, questions are developed by each organization or trainer based on their particular goals and interests. As a result, there is significant variation in what is measured. Originally, reactions referred only to how well a …

Decomposition Of Organic Peroxides And Hydrogen Peroxide By The Iron Thiolates And Related Complexes, Patrick H. Dussault, Andrew Olson

Department of Chemistry: Faculty Publications

Disclosed herein is a method of reducing or disproportionating peroxide, comprising combining an organic chalcogenide, an iron salt, and the peroxide in the presence of an additional reductant, which can be the organic chalcogenide. The method can be used to, e.g., prepare alcohols from peroxides and to disproportionate hydrogen peroxide into water and oxygen.

Nest Records Of Nebraska Birds, Wayne J. Mollhoff

Zea E-Books Collection

Nebraska Ornithologists' Union Occasional Paper Number 9

This publication is an attempt to provide a synopsis of the breeding information accumulated in the past two centuries. As with any compilation like this, other workers would likely come to different conclusions in choosing which records to accept and which to reject. I have tried to state the reasons for my decisions as clearly as possible. Most difficult to categorize are species which are not well documented. Hopefully by laying out the evidence I could find, others will be prompted to do more research, uncover definitive proof, and put more of our …

Optimizing Management Of Invasions In An Uncertain World Using Dynamic Spatial Models, Kim M. Pepin, Amy J. Davis, Rebecca S. Epanchin-Niell, Andrew M. Gormley, Joslin L. Moore, Timothy J. Smyser, H. Bradley Shaffer, William L. Kendall, Katriona Shea, Michael C. Runge, Sophie Mckee

United States Department of Agriculture Wildlife Services: Staff Publications

Dispersal drives invasion dynamics of nonnative species and pathogens. Applying knowledge of dispersal to optimize the management of invasions can mean the difference between a failed and a successful control program and dramatically improve the return on investment of control efforts. A common approach to identifying optimal management solutions for invasions is to optimize dynamic spatial models that incorporate dispersal. Optimizing these spatial models can be very challenging because the interaction of time, space, and uncertainty rapidly amplifies the number of dimensions being considered. Addressing such problems requires advances in and the integration of techniques from multiple fields, including ecology, …

An Appeal In Favor Of That Class Of Americans Called Africans, Lydia Maria Child, Paul Royster (Editor)

Zea E-Books in American Studies

The roots of white supremacy lie in the institution of negro slavery. From the 15th through the 19th century, white Europeans trafficked in abducted and enslaved Africans and justified the practice with excuses that seemed somehow to reconcile the injustice with their professed Christianity. The United States was neither the first nor the last nation to abolish slavery, but its proclaimed principles of freedom and equality were made ironic by the nation’s reluctance to extend recognition to all Americans.

“Americans” is what Mrs. Child calls those fellow countrymen of African ancestry in 1833; citizenship and equality were what she advocated …