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Taxonomy And Molecular Epidemiology Of Echinococcus Granulosus Sensu Lato, Thomas Romig, Dennis Ebi, Marion Wassermann

Harold W. Manter Laboratory of Parasitology: Library Materials

Echinococcus granulosus, formerly regarded as a single species with a high genotypic and phenotypic diversity, is now recognized as an assemblage of cryptic species, which differ considerably in morphology, development, host specificity (including infectivity/pathogenicity for humans) and other aspects. This diversity is reflected in the mitochondrial and nuclear genomes and has led to the construction of phylogenetic trees and hypotheses on the origin and geographic dispersal of various taxa. Based on phenotypic characters and gene sequences, E. granulosus (sensu lato) has by now been subdivided into E. granulosus sensu stricto (including the formerly identified genotypic variants G1-3), …

Sequence-Characterized Amplified Regions That Differentiate New World Screwworms From Other Potential Wound-Inhabiting Flies, Joan A Christen, Steven R. Skoda, Tiffany Heng-Moss, Donald J. Lee, John E. Foster

Department of Entomology: Faculty Publications

Abstract. New World screwworms, Cochliomyia hominivorax (Coquerel, 1858), were once devastating pests of warmblooded animals in the United States before they were successfully eradicated using the sterile insect technique. Guarding against the introduction of screwworms to North America or any other screwworm-free area relies on rapid, reliable identification of suspected cases. In the current study, the DNA from excised markers generated by randomly amplified polymorphic DNA polymerase chain reaction was used as the basis to generate 2 species-specific sequence-characterized amplified region molecular markers. Resulting primer pairs, named CR92A1 and J1A2 (each with forward and reverse components), produced amplicons of 852 …

Farmer Responses To Resistance Issues In Corn Rootworm To Bt Corn: Qualitative Analysis Of Focus Groups, Erin W. Hodgson, Robert Wright, Michael Gray, Thomas E. Hunt, Ken Ostlie, David A. Andow

Department of Entomology: Faculty Publications

Western corn rootworm is an important corn pest in the U.S. Some farmers noted unexpected corn rootworm injury of transgenic hybrids as early as 2008; however, the full extent of product performance is still not fully understood. We conducted telephone focus groups with farmers in 2013 to gain their perspective of current and future issues for corn rootworm. Respondents were surprised how quickly corn rootworm injury escalated in their fields and were disappointed with incorrect diagnoses from consultants and seed companies. Most participating farmers saw university Extension as an unbiased source of information.

Temporal And Spatial Variability In Dune Reactivation Across The Nebraska Sand Hills, Usa, Rebecca L. Schmeisser Mckean, Ronald J. Goble, Joseph B. Mason, James Swinehart, David B. Loope

Department of Earth and Atmospheric Sciences: Faculty Publications

The Nebraska Sand Hills is a stabilized dune field on the Great Plains of North America. Although it is well known that this dune field, like several others on the Great Plains, last experienced widespread activity during the Medieval Climatic Anomaly (MCA, ~AD 900–1300), spatial variation in the timing and nature of drought development is poorly constrained. To elucidate spatial trends in dune reactivation, samples potentially representing MCA activity across the Sand Hills were collected and dated using optically stimulated luminescence (OSL). Ages from the older part of the MCA were obtained from eolian sediments in the northwestern Sand Hills, …

Sandwich Enzyme-Linked Immunosorbent Assay For Detecting Sesame Seed In Foods, Stef J. Koppelman, Gülsen Söylemez, Lynn Niemann, Ferdelie E. Gaskin, Joseph L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Small amounts of sesame can trigger allergic reactions in sesame-allergic patients. Because sesame is a widely used food ingredient, analytical methods are needed to support quality control and food safety programs in the food industry. In this study, polyclonal antibodies against sesame seed proteins were raised, and an enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of sesame seed residue in food. A comparison was made between this ELISA and other assays, particularly focusing on recovery of sesame seed residue from different food matrices. The developed ELISA is sensitive with a lower limit of quantification of 0.5 …

Food Processing And Allergenicity, Kitty C. M. Verhoeckx, Yvonne M. Vissers, Joseph L. Baumert, Roland Faludi, Marcel Feys, Simon Flanagan, Corinne Herouet-Guicheney, Thomas Holzhauser, Ryo Shimojo, Nieke Van Der Bolt, Harry Wichers, Ian Kimber

Department of Food Science and Technology: Faculty Publications

Food processing can have many beneficial effects. However, processing may also alter the allergenic properties of food proteins. A wide variety of processing methods is available and their use depends largely on the food to be processed.

In this review the impact of processing (heat and non-heat treatment) on the allergenic potential of proteins, and on the antigenic (IgG-binding) and allergenic (IgE-binding) properties of proteins has been considered. A variety of allergenic foods (peanuts, tree nuts, cows’ milk, hens’ eggs, soy, wheat and mustard) have been reviewed.

The overall conclusion drawn is that processing does not completely abolish the allergenic …

The Microbiome Of Uncontacted Amerindians, Jose C. Clemente, Erica C. Pehrsson, Martin J. Blaser, Kuldip Sandhu, Zhan Gao, Bin Wang, Magda Magris, Glida Hidalgo, Monica Contreras, Óscar Noya-Alarcón, Orlana Lander, Jeremy Mcdonald, Mike Cox, Jens Walter, Phaik Lyn Oh, Jean F. Ruiz, Selena Rodriguez, Nan Shen, Se Jin Song, Jessica Metcalf, Rob Knight, Gautam Dantas, M. Gloria Dominguez-Bello

Department of Food Science and Technology: Faculty Publications

Most studies of the human microbiome have focused on westernized people with life-style practices that decrease microbial survival and transmission, or on traditional societies that are currently in transition to westernization. We characterize the fecal, oral, and skin bacterial microbiome and resistome of members of an isolated Yanomami Amerindian village with no documented previous contact with Western people. These Yanomami harbor a microbiome with the highest diversity of bacteria and genetic functions ever reported in a human group. Despite their isolation, presumably for >11,000 years since their ancestors arrived in South America, and no known exposure to antibiotics, they harbor …

How Good Is Good Enough? Data Requirements For Reliable Crop Yield Simulations And Yield-Gap Analysis, Patricio Grassini, Lenny G.J. Van Bussel, Justin Van Wart, Joost Wolf, Lieven Claessens, Haishun Yang, Hendrik Boogaard, Hugo De Groot, Martin K. Van Ittersum, Kenneth Cassman

Department of Agronomy and Horticulture: Faculty Publications

Numerous studies have been published during the past two decades that use simulation models to assess crop yield gaps (quantified as the difference between potential and actual farm yields), impact of climate change on future crop yields, and land-use change. However, there is a wide range in quality and spatial and temporal scale and resolution of climate and soil data underpinning these studies, as well as widely differing assumptions about cropping-system context and crop model calibration. Here we present an explicit rationale and methodology for selecting data sources for simulating crop yields and estimating yield gaps at specific locations that …

Using Swat To Simulate Crop Yields And Salinity Levels In The North Fork River Basin, Usa, Aaron R. Mittelstet, Daniel E. Storm, Art L. Stoecker

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Crop yields and salinity levels in the North Fork of the Red River (North Fork River) basin, located in southwestern Oklahoma and the Texas Panhandle, were analyzed based on the diverse climate in the region. Saline irrigation water is a major problem in the basin. The Elm Fork Creek flows through salt deposits, making the creek and its receiving stream, the North Fork River, too saline to use for irrigation. This greatly reduces the number of hectares that can be utilized for agricultural crops within the basin. A baseline SWAT model was setup, calibrated and validated to simulate streamflow and …

In The Eye Of The Cyclops: The Classic Case Of Cospeciation And Why Paradigms Are Important, Daniel R. Brooks, Eric P. Hoberg, Walter A. Boeger

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Scientific disagreements due to empirical problems—not enough data, not enough of the critical type of data, problems in analyzing the data—are generally short-lived and resolved in the next cycle of data production. Such disagreements are thus transitory in nature. Persistent scientific conflicts, on the other hand, do not necessarily mean some facts are correct and some are wrong, nor do they mean that we do not have enough information. More often, such persistent conflicts mean that the conceptual frameworks used by different groups of researchers are insufficient to resolve apparent conflicts in the data. The latter seems to be the …

Evolution In Action: Climate Change, Biodiversity Dynamics And Emerging Infectious Disease, Eric P. Hoberg, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Climatological variation and ecological perturbation have been pervasive drivers of faunal assembly, structure and diversification for parasites and pathogens through recurrent events of geographical and host colonization at varying spatial and temporal scales of Earth history. Episodic shifts in climate and environmental settings, in conjunction with ecological mechanisms and host switching, are often critical determinants of parasite diversification, a view counter to more than a century of coevolutionary thinking about the nature of complex host–parasite assemblages. Parasites are resource specialists with restricted host ranges, yet shifts onto relatively unrelated hosts are common during phylogenetic diversification of parasite lineages and directly …

Hymenolepis Folkertsi N. Sp. (Eucestoda: Hymenolepididae) In The Oldfield Mouse Peromyscus Polionotus (Wagner) (Rodentia: Cricetidae: Neotominae) From The Southeastern Nearctic With Comments On Tapeworm Faunal Diversity Among Deer Mice, Arseny A. Makarikov, Todd N. Nims, Kurt E. Galbreath, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A previously unrecognized species of hymenolepidid cestode attributable to Hymenolepis is described based on specimens in Peromyscus polionotus, oldfield mouse, from Georgia, United States, near the southeastern coast of continental North America. Specimens of Hymenolepis folkertsi n. sp. differ from those attributed to most other species in the genus by having testes arranged in a triangle and a scolex with a prominent rostrum-like protrusion. The newly recognized species is further distinguished by the relative position and length of the cirrus sac, shape of seminal receptacle, and relative size of external seminal vesicle and seminal receptacle. Hymenolepidid cestodes have sporadically …

Land Use/Land Cover Changes And Regional Climate Over The Loess Plateau During 2001-2009 – Part I. Observed Evidences, Rezaul Mahmood

School of Natural Resources: Faculty Publications

No abstract provided.

Lidar Scanning With Supplementary Uav Captured Images For Structural Inspections, Richard L. Wood, Mohammad Ebrahim Mohammadi

Department of Civil and Environmental Engineering: Faculty Publications

Structural assessment using remote sensing technologies can be performed efficiently and effectively using such technologies as LiDAR (light detection and ranging). LiDAR can be employed for various structural assessments, such as as-built conditions for a newly constructed facility, routine inspection during its service life, or structural collapse evaluation after a natural hazard or extreme event. However, the main disadvantage of LiDAR is that it is a line-of-sight technology that can result in significant occlusions. Architectural or structural components can be partially or fully occluded by another object with respect to the location of the laser scanner. Supplemental photogrammetry techniques, such …

Terrestrial Orchids In A Tropical Forest: Best Sites For Abundance Differ From Those For Reproduction, Melissa Whitman, James D. Ackerman

School of Biological Sciences: Faculty Publications

Suitable habitat for a species is often modeled by linking its distribution patterns with landscape characteristics. However, modeling the relationship between fitness and landscape characteristics is less common. In this study we take a novel approach towards species distribution modeling (SDM) by investigating factors important not only for species occurrence, but also abundance and physical size, as well as fitness measures. We used the Neotropical terrestrial orchid Prescottia stachyodes as our focal species, and compiled geospatial information on habitat and neighboring plants for use in a two-part conditional SDM that accounted for zero inflation and reduced spatial autocorrelation bias. First, …

Monthly Land Cover-Specific Evapotranspiration Models Derived From Global Eddy Flux Measurements And Remote Sensing Data, Yuan Fang, Ge Sun, Peter Caldwell, Steven G. Mcnulty, Asko Noormets, Jean-Christophe Domec, John King, Zhiqiang Zhang, Xudong Zhang, Guanghui Lin, Guangsheng Zhou, Jingfeng Xiao, Jiquan Chen

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Evapotranspiration (ET) is arguably the most uncertain ecohydrologic variable for quantifying watershed water budgets. Although numerous ET and hydrological models exist, accurately predicting the effects of global change on water use and availability remains challenging because of model deficiency and/or a lack of input parameters. The objective of this study was to create a new set of monthly ET models that can better quantify landscape-level ET with readily available meteorological and biophysical information. We integrated eddy covariance flux measurements from over 200 sites, multiple year remote sensing products from the Moderate Resolution Imaging Spectroradiometer (MODIS), and statistical modelling. Through examining …

Wildland Fire Deficit And Surplus In The Western United States, 1984–2012, Sean A. Parks, Carol Miller, Marc-André Parisien, Lisa M. Holsinger, Solomon Z. Dobrowski, John Abatzoglou

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Wildland fire is an important disturbance agent in the western US and globally. However, the natural role of fire has been disrupted in many regions due to the influence of human activities, which have the potential to either exclude or promote fire, resulting in a ‘‘fire deficit’’ or ‘‘fire surplus’’, respectively. In this study, we developed a model of expected area burned for the western US as a function of climate from 1984 to 2012.We then quantified departures from expected area burned to identify geographic regions with fire deficit or surplus. We developed our model of area burned as a …

Importance Of Succession, Harvest, And Climate Change In Determining Future Composition In U.S. Central Hardwood Forests, Wen J. Wang, Hong S. He, Frank R. Thompson Iii, Jacob S. Fraser, Brice B. Hanberry, William D. Dijak

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Most temperate forests in U.S. are recovering from heavy exploitation and are in intermediate successional stages where partial tree harvest is the primary disturbance. Changes in regional forest composition in response to climate change are often predicted for plant functional types using biophysical process models. These models usually simplify the simulation of succession and harvest and may not consider important species-specific demographic processes driving forests changes. We determined the relative importance of succession, harvest, and climate change to forest composition changes in a 125-million ha area of the Central Hardwood Forest Region of U.S. We used a forest landscape modeling …

An Individual-Based Model For Feral Hogs In Great Smoky Mountains National Park, Rene A. Salinas, William H. Stiver, Joseph L. Corn, Suzanne Lenhart, Charles Collins, Marguerite Madden, Kurt C. Vercauteren, Brandon S. Schmit, Ellen Kasari, Agricola Odoi, Graham Hickling, Hamish Mccallum

United States Department of Agriculture Wildlife Services: Staff Publications

The expansion of feral hog (Sus scrofa) populations in the United States has resulted in increased efforts to develop and implement control strategies designed to minimize the impacts done by this invasive species. We describe an individual-based model for feral hogs in Great Smoky Mountains National Park (GSMNP). The objectives of the model are to provide an understanding of the population dynamics of this feral hog population and to determine the efficacy of the annual harvest as a population control method. Results suggest that the dynamics of the population are driven by fall hard mast production and the …

Impacts Of Colonial Waterbirds On Vegetation And Potential Restoration Of Island Habitats, Christopher R. Ayers, Katie C. Hanson-Dorr, Sadie O'Dell, Charles D. Lovell, Michael L. Jones, Jason R. Suckow, Brian S. Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Colonial waterbirds have impacted forested island ecosystems throughout their breeding range, changing vegetation, and soil characteristics and bird communities. Our objectives were to (1) determine effects of three levels of colonial waterbird exclusion on overall vegetation diversity and growth, and survival of a candidate restoration species (black elderberry; Sambucus nigra canadensis); (2) investigate effects of different planting techniques on survival and growth of black elderberry; and (3) determine effects of waterbird colonization on soil chemistry. In 2012, we investigated effects of three levels of waterbird exclusion (none control plots [CON]; partial, which excluded waterbirds larger than gulls [PEX]; and …

Utility Of Mosquito Surveillance Data For Spatial Prioritization Of Vector Control Against Dengue Viruses In Three Brazilian Cities, Kim M. Pepin, Clint B. Leach, Cecilia Marques-Toledo, Karla H. Laass, Kelly S. Paixao, Angela D. Luis, David T.S. Hayman, Nels D. Johnson, Michael G. Buhnerkempe, Scott Carver, Daniel A. Grear, Kimberly Tsao, Alvaro E. Eiras, Colleen T. Webb

United States Department of Agriculture Wildlife Services: Staff Publications

Background: Vector control remains the primary defense against dengue fever. Its success relies on the assumption that vector density is related to disease transmission. Two operational issues include the amount by which mosquito density should be reduced to minimize transmission and the spatio-temporal allotment of resources needed to reduce mosquito density in a cost-effective manner. Recently, a novel technology, MI-Dengue, was implemented city-wide in several Brazilian cities to provide real-time mosquito surveillance data for spatial prioritization of vector control resources. We sought to understand the role of city-wide mosquito density data in predicting disease incidence in order to provide …

Rabies Surveillance Among Bats In Tennessee, Usa, 1996–2010, Amy T. Gilbert, Gary F. Mccracken, Lorinda L. Sheeler, Lisa I. Muller, Dorcas O'Rourke, William J. Kelch, John C. New Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

Rabies virus (RABV) infects multiple bat species in the Americas, and enzootic foci perpetuate in bats principally via intraspecific transmission. In recent years, bats have been implicated in over 90% of human rabies cases in the US. In Tennessee, two human cases of rabies have occurred since 1960: one case in 1994 associated with a tricolored bat (Perimyotis subflavus) RABV variant and another in 2002 associated with the tricolored/silver-haired bat (P. subflavus/ Lasionycteris noctivagans) RABV variant. From 1996 to 2010, 2,039 bats were submitted for rabies testing in Tennessee. Among 1,943 bats in satisfactory condition for testing and with a …

Modeling And Mapping The Probability Of Occurrence Of Invasive Wild Pigs Across The Contiguous United States, Meredith L. Mcclure, Christopher L. Burdett, Matthew L. Farnsworth, Mark W. Lutman, David M. Theobald, Philip D. Riggs, Daniel A. Grear, Ryan S. Miller

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa), also known as wild swine, feral pigs, or feral hogs, are one of the most widespread and successful invasive species around the world. Wild pigs have been linked to extensive and costly agricultural damage and present a serious threat to plant and animal communities due to their rooting behavior and omnivorous diet. We modeled the current distribution of wild pigs in the United States to better understand the physiological and ecological factors that may determine their invasive potential and to guide future study and eradication efforts. Using national-scale wild pig occurrence data reported between 1982 and …

Possible Tsunami Deposits On The Caribbean Coast Of The Yucatán Peninsula, Charles Shaw, Larry Benson

United States Geological Survey: Staff Publications

The western Caribbean Basin is notable for its tectonic stability. It has experienced no historical earthquakes or the tsunami that sometimes accompany them. This paper describes a single, wedge-shaped, boulder-covered, coastal berm on the Yucatán coast of México that stretches unbroken for 50 km across a coastal segment characterized by rocky headlands that alternate with crescent beaches. The remainder of the 350 km of Yucatán coast consists mostly of mangrove that extends 1 to 30 km inland, often behind long, sandy beaches. On the headlands, the surface of the berm is densely paved with boulders—large boulders on its seaward face …

Seasonal Shifts In Diet And Gut Microbiota Of The American Bison (Bison Bison), Gaddy T. Bergmann, Joseph Craine, Michael S. Robeson, Noah Fierer

United States Department of Agriculture Wildlife Services: Staff Publications

North American bison (Bison bison) are becoming increasingly important to both grassland management and commercial ranching. However, a lack of quantitative data on their diet constrains conservation efforts and the ability to predict bison effects on grasslands. In particular, we know little about the seasonality of the bison diet, the degree to which bison supplement their diet with eudicots, and how changes in diet influence gut microbial communities, all of which play important roles in ungulate performance. To address these knowledge gaps, we quantified seasonal patterns in bison diet and gut microbial community composition for a bison herd in …

Radiocarbon Dating Of Late Quaternary Sediments Using Fossil Gastropod Shells, Jeffrey S. Pigati, John P. Mcgeehin, Daniel R. Muhs

United States Geological Survey: Staff Publications

Terrestrial gastropods are one of the most successful groups of organisms on Earth. Their distribution includes all continents except Antarctica, and they occupy exceptionally diverse habitats, from marshes and wet meadows to alpine forests and Arctic tundra. Their shells are also commonly preserved in Quaternary deposits and potentially could be used for ¹⁴C dating. However, terrestrial gastropods are known to ingest limestone and incorporate the old carbon in their shells, resulting in apparent ages that are often too old. Recent studies have shown that many small (<1 cm diameter) terrestrial gastropods avoid this “limestone problem” even when living in areas in which carbonate rocks are readily available. However, the shells must also behave as closed systems with respect to carbon if their ages are to be considered reliable. Our latest work has been aimed at testing if small gastropod shells do, in fact, remain closed systems in late Quaternary deposits over a wide array of climate conditions across North America. Our results demonstrate that ages derived from Succineidae shells are identical to wood and charcoal ages in loess in Alaska, glacial deposits in the upper Midwest, loess in the Great Plains, and paleowetlands in the desert southwest. Moreover, Succineidae shell ages routinely fall within permitted limits set by stratigraphic boundaries, require less interpretation than humic acid ages that are commonly used in loess studies, can provide additional stratigraphic coverage to previous dating efforts, and maintain stratigraphic order more often than luminescence ages from the same stratigraphic intervals. Thus, we conclude that fossil Succineidae shells, and shells of a few other small gastropods, can be used for ¹⁴C dating regardless of the local lithology, past climate, or environmental …

Sea Level, Paleogeography, And Archeology On California's Northern Channel Islands, Leslie Reeder- Myers, Jon Erlandson, Daniel R. Muhs, Torben Rick

United States Geological Survey: Staff Publications

Sea-level rise during the late Pleistocene and early Holocene inundated nearshore areas in many parts of the world, producing drastic changes in local ecosystems and obscuring significant portions of the archeological record. Although global forces are at play, the effects of sea-level rise are highly localized due to variability in glacial isostatic adjustment (GIA) effects. Interpretations of coastal paleoecology and archeology require reliable estimates of ancient shorelines that account for GIA effects. Here we build on previous models for California's Northern Channel Islands, producing more accurate late Pleistocene and Holocene paleogeographic reconstructions adjusted for regional GIA variability. This region has …

Steps In Metagenomics: Let’S Avoid Garbage In And Garbage Out, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Is metagenomics a revolution or a new fad? Metagenomics is tightly associated with the availability of next-generation sequencing in all its implementations. The key feature of these new technologies, moving beyond the Sanger-based DNA sequencing approach, is the depth of nucleotide sequencing per sample.Knowing much more about a sample changes the traditional paradigms of “What is the most abundant?” or “What is the most significant?” to “What is present and potentially sig­nificant that might influence the situation and outcome?” Let’s take the case of identifying proper biomarkers of disease state in the context of chronic disease prevention. Prevention has been …

Geog 140: Introduction To Human Geography—A Peer Review Of Teaching Project Inquiry Portfolio, Katherine Nashleanas

UNL Faculty Course Portfolios

Large lecture classes of 100 students or more present unique challenges to both teaching and learning. The common method of “delivery” by instructors is lecture, often augmented by a set of Power Point slides; and, while the research literature shows that this is the more traditional way of teaching, increasingly it is becoming apparent that this method of instruction often does not reach students in the way we hope and often assume (Harvard Magazine 2015). Most of the students populating these classes are born of the Digital Age and have different expectations for learning, requiring new approaches in the classroom …

Going To A Home You’Ve Never Been To: The Return Migration Of Mexican And American-Mexican Children, Víctor Zúñiga, Edmund T. Hamann

Department of Teaching, Learning, and Teacher Education: Faculty Publications

The paper has two goals. The first is to present the main quantitative findings drawn from four surveys we conducted in Nuevo León (2004, n=14,473), Zacatecas (2005, n=11,258), Puebla (2009, n=18,829) and Jalisco (2010, n=11,479) using representative samples of children aged 7 to 16. We classified children in the following categories: (a) children who are returnees (who were born in Mexico), (b) international migrant children (born in the U.S.), and (c) mononational Mexicans. Among the second group, we distinguish children who had school experiences in the U.S. and those younger transnationals who came to Mexico before enrolling in school. The …