Digital Commons Network^™

Reflections On Plant And Soil Nematode Ecology: Past, Present And Future, Howard Ferris, Bryan S. Griffiths, Dorota L. Porazinska, Thomas O. Powers, Koon-Hui Wang, Mario Tenuta

Department of Plant Pathology: Faculty Publications

The purpose of this review is to highlight key developments in nematode ecology from its beginnings to where it stands today as a discipline within nematology. Emerging areas of research appear to be driven by crop production constraints, environmental health concerns, and advances in technology. In contrast to past ecological studies which mainly focused on management of plant-parasitic nematodes, current studies reflect differential sensitivity of nematode faunae. These differences, identified in both aquatic and terrestrial environments include response to stressors, environmental conditions, and management practices. Methodological advances will continue to influence the role nematodes have in addressing the nature of …

Chronic And Degenerative Diseases Similarities And Differences Between The Effects Of Epa And Dha On Markers Of Atherosclerosis In Human Subjects*, Darshan S. Kelley, Yuriko Adkins

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We have reviewed effects of long chain (LC) n-3 PUFA on markers of atherosclerosis in human subjects with a focus on individual effects of EPA and DHA. Initial results from epidemiological studies suggested that LC n-3 PUFA from fish oils (FO) reduced incidence of CVD; those results have been confirmed in interventional studies. Dietary intervention with n-3 PUFA decreased fasting and postprandial TAG, number of remnant-like chylomicron particles, large VLDL, and total and small dense LDL particles. It increased mean size of LDL particles by increasing number of large and decreasing those of small dense particles. With some exceptions, n-3 …

Five New Records Of Ants (Hymenoptera: Formicidae) For Nebraska, Kristine T. Nemec, James C. Tager, Elizabeth Manley, Craig R. Allen

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Ants are ubiquitous and influential organisms in terrestrial ecosystems. About 1,000 ant species occur in North America, where they are found in nearly every habitat (Fisher and Cover 2007). Ants are critical to ecological processes and structure. Ants affect soils via tunneling activity (Baxter and Hole 1967), disperse plant seeds (Lengyel et al. 2009), prey upon a variety of insects and other invertebrates (Way and Khoo 1992, Folgarait 1998), are often effective primary consumers through their prodigious consumption of floral and especially extrafloral nectar, and honeydew (Tobin 1994), and serve as prey for invertebrates (Gotelli 1996, Gastreich 1999) and vertebrates …

Population Estimate Of Chinese Mystery Snail (Bellamya Chinensis) In A Nebraska Reservoir, Noelle M. Chaine, Craig R. Allen, Kent A. Fricke, Danielle M. Haak, Michelle L. Hellman, Robert A. Kill, Kristine T. Nemec, Kevin L. Pope, Nicholas A. Smeenk, Bruce J. Stephen, Daniel R. Uden, Kody M. Unstad, Ashley E. Vanderham

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The Chinese mystery snail (Bellamya chinensis) is an aquatic invasive species in North America. Little is known regarding this species’ impacts on freshwater ecosystems. It is believed that population densities can be high, yet no population estimates have been reported. We utilized a mark-recapture approach to generate a population estimate for Chinese mystery snail in Wild Plum Lake, a 6.47-ha reservoir in southeast Nebraska. We calculated, using bias-adjusted Lincoln-Petersen estimation, that there were approximately 664 adult snails within a 127 m² transect (5.2 snails/m²). If this density was consistent throughout the littoral zone (depth) of …

Spatial Complexity In Fragmenting Amazonian Rainforests: Do Feedbacks From Edge Effects Push Forests Towards An Ecological Threshold?, Graeme S. Cumming, Jane Southworth, Xanic J. Rondon, Matthew Marsik

United States Department of Commerce: Staff Publications

Deforestation and resulting landscape fragmentation are important concerns in many tropical areas. Deforestation is a complex process with many potential feedback loops, many of which are ignored in models that attempt to interpolate forest loss based on past deforestation rates. In addition, most ecological studies of the impacts of deforestation have focused on landscapes that are already fragmented. These studies ignore the fact that edge effects, such as anthropogenic fire, reach their maximum well before habitat connectivity is lost and may create positive feedbacks that result in further fragmentation. We developed a simple model to explore the potential influence of …

Linking Ciguatera Poisoning To Spatial Ecology Of Fish: A Novel Approach To Examining The Distribution Of Biotoxin Levels In The Great Barracuda By Combining Non-Lethal Blood Sampling And Biotelemetry, Amanda C. O'Toole, Marie-Yasmine Dechraoui Bottein, Andy J. Danylchuk, John S. Ramsdell, Steven J. Cooke

United States Department of Commerce: Staff Publications

Ciguatera in humans is typically caused by the consumption of reef fish that have accumulated Ciguatoxins (CTXs) in their flesh. Over a six month period, we captured 38 wild adult great barracuda (Sphyraena barracuda), a species commonly associated with ciguatera in The Bahamas. We sampled three tissues (i.e.,muscle, liver, and blood) and analysed them for the presence of ciguatoxins using a functional in vitro N2A bioassay. Detectable concentrations of ciguatoxins found in the three tissue types ranged from 2.51 to 211.74 pg C-CTX-1 equivalents/ g. Blood and liver toxin concentrations were positively correlated (ρ=0.86, P=0.003), indicating …

Pond-Level Risk Factors Associated With Columnaris Disease On Mississippi Commercial Catfish Farms, Fred L. Cunningham, Skip W. Jack, David Hardin, Robert W. Wills

United States Department of Agriculture Wildlife Services: Staff Publications

A large commercial catfish enterprise encompassing over 500 food fish ponds from five farms covering multiple counties in the Mississippi Delta was included in this analysis of columnaris risk factors. A gram-negative bacterium, Flavobacterium columnare, is the cause of columnaris disease and is considered the second-most prevalent bacterial disease in farm-raised catfish. The objective of this study was to determine if pond-level risk factors reported by farm personnel were associated with columnaris disease mortalities. To identify risk factors affecting susceptibility of farm-raised channel catfish Ictalurus punctatus to columnaris disease, a Catfish Management database was developed. Logistic regression was used …

Coyote (Canis Latrans), 100+ Years In The East: A Literature Review, Lauren L. Mastro, Eric M. Gese, Julie K. Young, John A. Shivik

United States Department of Agriculture Wildlife Services: Staff Publications

Coyotes (Canis latrans) have expanded into the eastern United States over the last 100 years. Increases in their distribution and abundance have been documented and concerns about their presence in urban areas and their impact on both native wildlife and domestic livestock are growing. These impacts require further investigation and may require changes to management strategies. Two documents, a book and a technical bulletin, provide general overviews of eastern coyote biology. However, these documents are not comprehensive, and are either not readily available or were published >15 years ago. We provide a comprehensive review of the existing literature …

Vegetation Outlook (Vegout): Predicting Remote Sensing–Based Seasonal Greenness, Tsegaye Tadesse, Brian D. Wardlow, Mark D. Svoboda, Michael J. Hayes

National Drought Mitigation Center: Faculty Publications

Accurate and timely prediction of vegetation conditions enhances knowledge-based decision making for drought planning, mitigation, and response. This is very important in countries that are highly dependent on rainfed agriculture. For example, studies show that remote sensing–based observations and vegetation condition prediction have great potential for estimating crop yields (Verdin and Klaver, 2002; Ji and Peters, 2003; Seaquist et al., 2005; Tadesse et al., 2005a, 2008; Funk and Brown, 2006), which in turn may help to address agricultural development and food security issues, as well as improve early warning systems.

Many studies have demonstrated the value of Vegetation Indices (VIs), …

Seasonal Variation Of Coyote Diet In Northwestern Wyoming: Implications For Dietary Overlap With Canada Lynx?, Jennifer L. B. Dowd, Eric M. Gese

United States Department of Agriculture Wildlife Services: Staff Publications

Exploitative competition through resource utilization may occur between coyotes (Canis latrans) and other carnivores. In the southern periphery of Canada lynx (Lynx canadensis) range, there is concern that increased snowmobile activity may enable coyotes to increase their movements into deep snow areas during the winter months, thereby potentially creating heightened resource competition with lynx, mainly for the lynx’s main prey, snowshoe hares (Lepus americanus). We studied the seasonal variation of coyote diets and the dietary overlap between coyotes and lynx in a 512-km² high-elevation study area in northwestern Wyoming. Dietary shifts by coyotes …

Using A Population Growth Model To Simulate Response Of Plodia Interpunctella Hubner To Temperature And Diet, Emily A. Fontenot, Frank H. Arthur, James R. Nechols, James E. Throne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Response to temperature and diet are major factors in the potential population growth of Plodia interpunctella Hubner, a damaging pest of many stored products. A population growth model was used to simulate population development on an optimal wheat-based diet and a sub-optimal diet of raisins at 20–35°C, using different starting values for initial density and life stage. Predicted population development on the two diets increased with temperature and growth patterns were similar regardless of starting population levels at temperatures over 20°C. The predicted population levels on raisins were much less than those predicted for wheat diet, with the same general …

Historical Perspectives On Avhrr Ndvi And Vegetation Drought Monitoring, Assaf Anyamba, Compton J. Tucker

United States National Aeronautics and Space Administration: Publications

Satellite measurements of the biosphere have now become common place in various aspects of large-scale environmental monitoring, including drought and crop monitoring. This was not the case until the launch of the Advanced Very High Resolution Radiometer (AVHRR) instrument on June 27, 1979, on board the first National Oceanic and Atmospheric Administration (NOAA) Advanced Television Infrared Observation Satellite (TIROS-N/NOAA-6) polar-orbiting satellite. Initially, the NOAA AVHRR satellites were designed to observe the earth’s weather patterns: primarily cloud dynamics, vertical soundings of the atmosphere, and sea surface temperatures. Early studies on remote sensing of vegetation were focused on understanding seasonality. The vernal …

Nepetalactones From Essential Oil Of Nepeta Cataria Represent A Stable Fly Feeding And Oviposition Repellent, J. J. Zhu, Dennis R. Berkebile, C. E. Dunlap, A. Zhang, D. Boxler, K. Tangtrakulwanich, R. Behle, Frederick P. Baxendale, Gary J. Brewer

Department of Entomology: Faculty Publications

The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), is one of the most serious pests to livestock. It feeds mainly on cattle and causes significant economic losses in the cattle industry. Standard stable fly control involving insecticides and sanitation is usually costly and often has limited effectiveness. As we continue to evaluate and develop safer fly control strategies, the present study reports on the effectiveness of catnip (Nepeta cataria L.) oil and its constituent compounds, nepetalactones, as stable fly repellents. The essential oil of catnip reduced the feeding of stable flies by >96% in an in vitro bioassay system, …

Clinical Use Of Probiotics In Pediatric Allergy (Cuppa): A World Allergy Organization Position Paper, Alessandro Fiocchi, Wesley Burks, Sami L. Bahna, Leonard Bielory, Robert J. Boyle, Renata Cocco, Sten Dreborg, Richard E. Goodman, Mikael Kuitunen, Tari Haahtela, Ralf G. Heine, Gideon Lack, David A. Osborn, Hugh Sampson, Gerald W. Tannock, Bee Wah Lee

Department of Food Science and Technology: Faculty Publications

Background: Probiotic administration has been proposed for the prevention and treatment of specific allergic manifestations such as eczema, rhinitis, gastrointestinal allergy, food allergy, and asthma. However, published statements and scientific opinions disagree about the clinical usefulness.

Objective: A World Allergy Organization Special Committee on Food Allergy and Nutrition review of the evidence regarding the use of probiotics for the prevention and treatment of allergy.

Methods: A qualitative and narrative review of the literature on probiotic treatment of allergic disease was carried out to address the diversity and variable quality of relevant studies. This variability precluded systematization, and an expert …

Next-Generation Sequencing Reveals Significant Bacterial Diversity Of Botrytized Wine, Nicholas A. Bokulich, C. M. Lucy Joseph, Greg Allen, Andrew K. Benson, David A. Mills

Department of Food Science and Technology: Faculty Publications

While wine fermentation has long been known to involve complex microbial communities, the composition and role of bacteria other than a select set of lactic acid bacteria (LAB) has often been assumed either negligible or detrimental. This study served as a pilot study for using barcoded amplicon next-generation sequencing to profile bacterial community structure in wines and grape musts, comparing the taxonomic depth achieved by sequencing two different domains of prokaryotic 16S rDNA (V4 and V5). This study was designed to serve two goals: 1) to empirically determine the most taxonomically informative 16S rDNA target region for barcoded amplicon sequencing …

Development Of Camera Technology For Monitoring Nests, W. Andrew Cox, M. Shane Pruett, Thomas J. Benson, Scott J. Chiavacci, Frank R. Thompson Iii

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Photo and video technology has become increasingly useful in the study of avian nesting ecology. However, researchers interested in using camera systems are often faced with insufficient information on the types and relative advantages of available technologies. We reviewed the literature for studies of nests that used cameras and summarized them based on study objective and the type of technology used. We also designed and tested two video systems that we used for three nest predator and behavioral studies. We found 327 studies that recorded 255 bird species spanning 19 orders. Cameras were most commonly used to study nest predators …

Quantifying Tropical Dry Forest Type And Succession: Substantial Improvement With Lidar, Sebastian Martinuzzi, William A. Gould, Lee A. Vierling, Andrew T. Hudak, Ross F. Nelson, Jeffrey S. Evans

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Improved technologies are needed to advance our knowledge of the biophysical and human factors influencing tropical dry forests, one of the world’s most threatened ecosystems. We evaluated the use of light detection and ranging (LiDAR) data to address two major needs in remote sensing of tropical dry forests, i.e., classification of forest types and delineation of forest successional status. We evaluated LiDAR-derived measures of three-dimensional canopy structure and subcanopy topography using classification-tree techniques to separate different dry forest types and successional stages in the Guánica Biosphere Reserve in Puerto Rico. We compared the LiDARbased results with classifications made from …

Yersinia Pestis: Examining Wildlife Plague Surveillance In China And The Usa, Sarah N. Bevins, John A. Baroch, Dale L. Nolte, Min Zhang, Hongxuan He

United States Department of Agriculture Wildlife Services: Staff Publications

Plague is a zoonotic disease caused by the bacterium Yersinia pestis Lehmann and Neumann, 1896. Although it is essentially a disease of rodents, plague can also be transmitted to people. Historically, plague has caused massive morbidity and mortality events in human populations, and has recently been classified as a reemerging disease in many parts of the world. This public health threat has led many countries to set up wild and domestic animal surveillance programs in an attempt to monitor plague activity that could potentially spill over into human populations. Both China and the USA have plague surveillance programs in place, …

Covariation Between Understorey Light Environments And Soil Resources In Bornean Mixed Dipterocarp Rain Forest, Sabrina E. Russo, Lin Zhang, Sylvester Tan

School of Biological Sciences: Faculty Publications

Variation in understorey irradiance is both a cause and consequence of the structure and dynamics of closed-canopy forests, which are also influenced by soil nutrients and water availability. We tested the hypothesis that understorey light regimes differ among four mixed dipterocarp forest types that share the same rainfall, but grow on different soils along an edaphic gradient at one site in Borneo. Based on data from photosynthetically active radiation sensors deployed at 1-m height at 36 locations for 351 sensor-days, we found significant soil-related variation in irradiance. The more productive forest types on clay and fine loam had lower daily …

North American Birds As Potential Amplifying Hosts Of Japanese Encephalitis Virus, Nicole M. Nemeth, Angela Bosco-Lauth, Paul T. Oesterle, Dennis Kohler, Richard Bowen

United States Department of Agriculture Wildlife Services: Staff Publications

Japanese encephalitis virus (JEV) is an emerging arbovirus, and inter-continental spread is an impending threat. The virus is maintained in a transmission cycle between mosquito vectors and vertebrate hosts, including birds. We detected variation in interspecies responses among North American birds to infection with strains of two different JEV genotypes (I and III). Several native North American passerine species and ring-billed gulls had the highest average peak viremia titers after inoculation with a Vietnamese (genotype I) JEV strain. Oral JEV shedding was minimal and cloacal shedding was rarely detected. The majority of birds, both viremic (72 of 74; 97.3%) and …

Didactic Lessons Derived From The Shoshone River Skunk Rabies Epizootic, Craig A. Ramey, Kenneth H. Mills, Justin W. Fischer, Robert G. Mclean

United States Department of Agriculture Wildlife Services: Staff Publications

Studying the lower Shoshone River Basin’s (SRB) striped skunk (Mephitis mephitis) rabies 6-year epizootic in northwestern Wyoming has produced four didactic lessons. First, physiographic changes by settlers circa 1900 affected its zoogeography by creating a canal system for irrigating crops originating at Buffalo Bill Reservoir (BBR). The resulting landscape changes increased agricultural lands and skunk habitat eightfold between the valley’s steep gravel benches. The valley was historically free of skunk rabies until the epizootic’s index case in August 1988. Second, human intervention began when the Bighorn County Predator Board (BCPB) proactively implemented rabies trapping surveillance and depopulation programs …

The Relationship Between Variable Host Grouping And Functional Responses Among Parasitoids Of Antispila Nysaefoliella (Lepidoptera: Heliozelidae), Candace Low, Sonja J. Scheffer, Matthew L. Lewis, Michael W. Gates

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Our study investigated the importance of variability in the parasitoid community as a source of selection on host group size using a field population of the tupelo leafminer, Antispila nysaefoliella Clemens, which specializes on tupelo, Nyssa sylvatica Marsh. Larvae were collected from leaves with variable numbers of larvae and screened for parasitism using polymerase chain reaction of mitochondrial cytochrome oxidase I using markers designed specifically for amplifying parasitoid DNA while excluding host DNA. This method of selective PCR was effective for detecting the presence and identifying species of immature stages of three hymenopteran superfamilies: Chalcidoidea, Ichneumonoidea and Platygastroidea, which represented …

Gene Transcription In Sea Otters (Enhydra Lutris); Development Of A Diagnostic Tool For Sea Otter And Ecosystem Health, Lizabeth Bowen, A. Keith Miles, Michael Murray, Martin Haulena, Judy Tuttle, William Van Bonn, Lance Adams, James L. Bodkin, Brenda Ballachey, James A. Estes, M. Tim Tinker, Robin Keister, Jeffrey L. Stott

United States Geological Survey: Staff Publications

Gene transcription analysis for diagnosing or monitoring wildlife health requires the ability to distinguish pathophysiological change from natural variation. Herein, we describe methodology for the development of quantitative real-time polymerase chain reaction (qPCR) assays to measure differential transcript levels of multiple immune function genes in the sea otter (Enhydra lutris); sea otter-specific qPCR primer sequences for the genes of interest are defined. We establish a ‘reference’ range of transcripts for each gene in a group of clinically healthy captive and free-ranging sea otters. The 10 genes of interest represent multiple physiological systems that play a role in immuno-modulation, …

Partial Systematic Adaptive Cluster Sampling, Arthur L. Dryver, Urairat Netharn, David R. Smith

United States Geological Survey: Staff Publications

No abstract provided.

Genetic Measures Confirm Familial Relationships And Strengthen Study Design, Stacie J. Robinson, Ryan D. Walrath, Timonthy R. Vandeelen, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Social structure and behavioral interactions between individuals shape basic biological processes, such as breeding; foraging and predator avoidance; movement and dispersal; and disease transmission. We used a targeted trapping strategy to capture kin groups of white-tailed deer (Odocoileus virginianus) during 2007 and 2008 in Sandhill Wildlife Research Area, Wisconsin, USA, in order to observe social behaviors. Because inferring family relationships from observation of behavior is subjective, we usedmeasures of genetic relatedness and parentage assignment tests to determine that our capture strategy was efficient for capturing related pairs (78% of groups contained 1 dyad of related animals). The results of …

Efficacy Of Proximity Loggers For Detection Of Contacts Between Maternal Pairs Of White-Tailed Deer, Ryan Walrath, Timonthy R. Vandeelen, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Contact frequency and duration estimates between individuals are important to understanding the behavioral ecology of wildlife species and the epidemiology of infectious diseases. A new technology uses proximity data loggers to record time and duration of contacts. We conducted an experiment at Sandhill Wildlife Management Area, located near Babcock, Wisconsin (USA) to compare probabilities of detecting intraspecific contacts among white-tailed deer (Odocoileus virginianus) maternal pairs (dams/fawns) based on detections from proximity loggers deployed on collars versus those obtained from direct observation. We defined 5 discrete probabilities of detection of a contact in terms of P (probability of detection …

Susceptibility Of Stored-Product Psocids To Aerosol Insecticides, George P. Opit, Frank H. Arthur, James E. Throne, Mark E. Payton

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The efficacies of commercial methoprene and esfenvalerate aerosols for control of stored-product psocid pests were evaluated in simulated field studies. The efficacies of methoprene, esfenvalerate EC, the carrier Isopar-M™, and a combination of methoprene and esfenvalerate aerosols for control of Liposcelis decolor (Pearman) (Psocoptera: Liposcelididae) and Liposcelis entomophila (Enderlein) nymphs were assessed, and the effects of direct and indirect exposure of Liposcelis bostrychophila Badonnel, L. decolor, and Liposcelis paeta Pearman adults to esfenvalerate EC aerosol were evaluated. The greatest nymphal mortality attained was 76%, indicating that the four aerosols tested were ineffective against L. decolor and L. entomophila nymphs. …

Genetic Structure Of Grass Carp Populations In The Missouri And Mississippi River Basins, Usa, Bobbi M. Adams, Katie N. Bertrand, Michael L. Brown, Donald Auger

The Prairie Naturalist

We provided an early characterization of the genetic structure of the grass carp (Ctenopharyngodon idella) population as it expands its distribution in both the Missouri and Mississippi River basins. Further, we provided initial comparisons of allelic richness at 17 polymorphic microsatellite markers between 56 grass carp from the USA, and six from the Yangtze River in China. The number of alleles per locus ranged from two to eight and size ranges of alleles for fish collected from the invaded and native ranges were similar (P ≤ 0.001; 107–226 bp) to those previously reported in the literature. Distance-based clustering …

Msh1 Influence On Plant Mitochondrial Genome Recombination And Phenotype In Tobacco, Peibei Sun

School of Biological Sciences: Dissertations, Theses, and Student Research

Plant mitochondrial genomes are composed of unusually complex structures, due to active recombination at numerous repeated sequences in the genome. The maintenance of mitochondrial genome stability is under the control of identifiable nuclear genes. In plants, three nuclear genes (MSH1, RECA3 and OSB1) have been shown to participate in recombination surveillance and the suppression of illegitimate recombination in mitochondria. Disruption of these loci in Arabidopsis results in reproducible mitochondrial genome rearrangements. We demonstrated that repeat-mediated de novo recombination was also enhanced in both Arabidopsis and tobacco during passage through in vitro culture. Furthermore, in vitro conditions led …

Tracking Large Carnivore Dispersal Using Isotopic Clues In Claws: An Application To Cougars Across The Great Plains, Viviane Hénaux, Larkin A. Powell, Keith A. Hobson, Clayton Kent Nielsen, Michelle A. Larue

School of Natural Resources: Faculty Publications

1. Cougar (Puma concolor) populations, like other large carnivores, have increased during recent decades and may be recolonizing their former ranges in Midwestern North America. The dispersal routes taken by these animals from established populations are unknown and insight into these movements would facilitate their conservation and management.

2. We inferred the origin and migration route of four dispersing cougars using stable hydrogen (δD) and carbon (δ¹³C) isotope values along one of their claws. We compared isotopic variations within claws to regional and large-scale isoscapes of δD and δ¹³C values in prey species. Using …