Digital Commons Network^™

Integrated Multi-Omics Analysis Of Cerebrospinal Fluid In Postoperative Delirium, Bridget A. Tripp, Simon T. Dillon, Min Yuan, John M. Asara, Sarinnapha M. Vasunilashorn, Tamara G. Fong, Sharon K. Inouye, Long H. Ngo, Edward R. Marcantonio, Zhongcong Xie, Towia A. Libermann, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

Preoperative risk biomarkers for delirium may aid in identifying high-risk patients and developing intervention therapies, which would minimize the health and economic burden of postoperative delirium. Previous studies have typically used single omics approaches to identify such biomarkers. Preoperative cerebrospinal fluid (CSF) from the Healthier Postoperative Recovery study of adults ≥ 63 years old undergoing elective major orthopedic surgery was used in a matched pair delirium case–no delirium control design. We performed metabolomics and lipidomics, which were combined with our previously reported proteomics results on the same samples. Differential expression, clustering, classification, and systems biology analyses were applied to individual …

Revision Of Trachyderines Related To Sphaenothecus From North America With Description Of Three New Genera, And New Species Of Lophalia Casey, 1912, Mannophorus Leconte, 1854, And Ischnocnemis Thomson, 1864 (Coleoptera: Cerambycidae: Cerambycinae: Trachyderini), Bryan K. Eya

Insecta Mundi

The genera Lophalia Casey, 1912, Mannophorus LeConte, 1854 and Ischnocnemis Thomas, 1864 (Coleoptera: Cerambycidae: Cerambycinae: Trachyderini) are recharacterized by providing additional morphological features to enhance the delineation of these genera. Two species, Lophalia cribricollis Bates, 1892 and Ischnocnemis luteicollis (Bates, 1885), are transferred to a new genus Lophaliamorpha Eya with Lophaliamorpha luteicollis (Bates) new combination as the type species. Ischnocnemis skillmani Chemsak and Hovore, 2010 is transferred to a new genus Paramannophorus Eya with Paramannophorus skillmani (Chemsak and Hovore) new combination as the type species. A new genus Microteroschema is created to accommodate two additional species from Mexico: Microteroschema parvum …

Three Essays In Food Security And The U.S. Sugar Program, Jacob Michels

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Chapter one devises an approach to adjust estimates of the number of food insecure for changes in sedentarism over time. Existing methodologies are biased upwards and date back several decades. We build a household model utilizing a Stone-Geary utility function, which rationalizes households shifting their labor decisions towards sedentary activities. Our comparative statics examine the impacts of changes in the productivities of sedentary types, as opposed to my physically demanding types, of activities and also their returns. Our empirical approach is informed by our theoretical model and comparative statics, in which we construct a unique pseudo-panel dataset. Sitting time serves …

Optimized Gadolinium-Do3a Loading In Raftpolymerized Copolymers For Superior Mr Imaging Of Aging Blood–Brain Barrier, Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest Kievit, Anthony J. Covertine

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The development of gadolinium-based contrast agents (GBCAs) has been pivotal in advancing magnetic resonance imaging (MRI), offering enhanced soft tissue contrast without ionizing radiation exposure. Despite their widespread clinical use, the need for improved GBCAs has led to innovations in ligand chemistry and polymer science. We report a novel approach using methacrylate-functionalized DO3A ligands to synthesize a series of copolymers through direct reversible addition-fragmentation chain transfer (RAFT) polymerization. This technique enables precise control over the gadolinium content within the polymers, circumventing the need for subsequent conjugation and purification steps, and facilitates the addition of other components such as targeting ligands. …

Using Machine Learning To Determine A Functional Classifier Of Reward Responsiveness And Its Association With Adolescent Psychiatric Symptomatology, Robert James Richard Blair, Johannah Bashford-Largo, Ahria Dominguez, Matthew Dobbertin, Karina S. Blair, Sahil Bajaj

Center for Brain, Biology, and Behavior: Faculty Publications

Background. Machine learning (ML) has developed classifiers differentiating patient groups despite concerns regarding diagnostic reliability. An alternative strategy, used here, is to develop a functional classifier (hyperplane) (e.g. distinguishing the neural responses to received reward v. received punishment in typically developing (TD) adolescents) and then determine the functional integrity of the response (reward response distance from the hyperplane) in adolescents with externalizing and internalizing conditions and its associations with symptom clusters.

Methods. Two hundred and ninety nine adolescents (mean age = 15.07 ± 2.30 years, 117 females) were divided into three groups: a training sample of TD adolescents …

Second Language Learning In Older Adults Modulates Stroop Task Performance And Brain Activation, Douglas H. Schultz, Alison Gansemer, Kiley Allgood, Mariah Gentz, Lauren Secilmis, Zoha Deldar, Cary R. Savage, Ladan Ghazi Saidi

Center for Brain, Biology, and Behavior: Faculty Publications

Introduction: Numerous studies have highlighted cognitive benefits in lifelong bilinguals during aging, manifesting as superior performance on cognitive tasks compared to monolingual counterparts. Yet, the cognitive impacts of acquiring a new language in older adulthood remain unexplored. In this study, we assessed both behavioral and fMRI responses during a Stroop task in older adults, pre- and post language-learning intervention.

Methods: A group of 41 participants (age: 60–80) from a predominantly monolingual environment underwent a four-month online language course, selecting a new language of their preference. This intervention mandated engagement for 90 minutes a day, five days a week. …

A Two-Part Project, Phil Warburton

Department of Entomology: Distance Master of Science Projects

1) an analysis of the constraints to the use of entomological observations collected by citizen scientists for research

The online database, iNaturalist, is transforming observational entomology with a massive and valuable database of photographed insect sightings in their environment. Increasingly, iNaturalist is becoming a rich source of scientific data for research purposes. However, iNaturalist faces a hidden but potentially serious crisis-in-the making. The phenomenal growth threatens to outstrip the capacity of expert insect identifiers.

As the database grows rapidly, the rate of identification of insect observations to the “Research Grade” standard is in decline. Furthermore, the number of expert identifiers …

Evaluating A Precision Sprayer For Detecting Weeds And Spraying Herbicides In Real Time (Spot Spray) For Weed Management In Corn And Soybean, Adam Eric Leise

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Herbicide sprayers are becoming larger and more efficient in applications in crop fields. A precise and efficient herbicide application is advantageous in effective weed control. New precision sprayers were introduced in hopes of reducing the amount of herbicide applied. Greeneye Technology and John Deere have developed precision sprayers for the detection of weeds in crop fields, which come equipped with a dual tank system that allows for two different herbicides to be separated from tank to tip. The objectives of this study were to evaluate the broadleaf and grass weed control efficacy of spot spray (SS) technology when applied in …

Triticale As A Source Of Resistance To Wheat Streak Mosaic Virus And Triticum Mosaic Virus, Marcos Winicius Goncalves De Souza

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) present a great threat to wheat production in the Great Plains area of the United States due to the loss of photosynthetic area causing reductions in crop yields. Effective control of these viruses is limited to a few available strategies including controlling volunteer wheat and using currently identified resistant genes to both the vector and viruses. While effective, these genes show temperature sensitivity and yield drag, and evidence that the vectors and viruses can overcome them has been found. Thus, new methods of genetic resistance are urgently needed. Field observations …

Utilization Of Probiotics To Compete With Clostridioides Difficile For Nutrient-Niches In A Variety Of In Vitro Contexts, April Elizabeth Johnson

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Clostridium difficile is a major threat to public health and primarily affects at risk groups such as elderly, immunocompromised individuals, and individuals on antibiotics. Many decades of research have been conducted to figure out ways to exclude or reduce C. difficile colonization and toxin production. Two major proposed mechanisms for colonization resistance are production of secondary bile salts and competition for nutrients.

In Chapter 2, we found that antibiotic treatment disrupted bile salt metabolism increasing the levels of the primary bile salt cholate and decreasing the levels of deoxycholate, a secondary bile salt. However, bile salts were not required …

Biostratigraphic Revision Of Esthonyx (Tillidontia, Mammalia) In The Contect Of Climate Change In The Lower Eocene Of The Bighorn Basin, Wyoming, John Colter Johnson

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Esthonyx is an early Eocene tillodont found in North America, Europe, and India. The genus was named by Edward Drinker (E. D.) Cope in 1874 based on specimens from the San Jose Formation in New Mexico. Since then, several species of Esthonyx have been described from North America alone. The best record of Esthonyx comes from the central Bighorn Basin (BHB) in Wyoming, where it appears in 220 localities in a 640 m-thick stratigraphic succession. Since the last summary of Esthonyx in the BHB, several hundred new specimens have been collected and curated at the Denver Museum of Nature and …

Multi-Case Study Of Left-Flank Boundaries Within Supercells, Peyton B. Stevenson

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

This study investigates the prevalence and significance of forward-flank convergence boundaries (FFCBs) and left-flank convergence boundaries (LFCBs) in shaping the structure and intensity of supercells, using observational data from various field projects. Unlike previous research focusing on individual cases, this study examines a diverse range of cases to provide comprehensive insights into the relationship between these boundaries and supercell characteristics such as intensity, longevity, and tornadogenesis. By analyzing high-resolution surface data, the research addresses the frequency, location, and intensity of these boundaries, and their impact on pseudo vertical vorticity, pseudo convergence, and density gradients. A total of 228 boundary identifications …

Examining Dietary Disparities And Associated Factors Among Young Children Across Diverse Racial, Ethnic And Immigrant Households In Nebraska, Naveta Bhatti

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Early childhood is a crucial stage for developing eating behaviors which in turn plays a major role in determining health outcomes. Assessing children’s diet plays a crucial role in tackling the obesity epidemic. One of the ways to assess children’s dietary intake is by evaluating their dietary quality. Dietary quality refers to the overall healthfulness and nutritional value of an individual's diet, considering the combination of foods consumed over time. Research has consistently demonstrated that a high-quality diet is associated with a reduced risk of several health issues, such as all-cause mortality, cardiovascular disease incidence or mortality, cancer incidence or …

Party Over Democracy? When Do Americans Stand By Democratic Principles, Zenat Ahmed

Department of Political Science: Dissertations, Theses, and Student Research

Studies show that people support their party’s representatives even when their behavior violates fundamental democratic principles for partisan gain. We test the effects of two interventions- each randomly and independently assigned—to investigate the conditions under which people will prioritize democracy over party goals. In the first intervention, we explicitly point out how an in-party politician’s behavior (e.g., banning public gatherings of their opponents) violates a core democratic principle (e.g., free assembly). The second intervention is a question-order manipulation to test if participants are less tolerant of politicians’ antidemocratic behavior when support for democratic principles is reported first. We find that …

Unmanned Aerial Vehicle (Uav)-Based High-Throughput Phenotyping For Maize Improvement, Eric T. Rodene

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Modern breeding programs rely heavily on efficiently screening large numbers of genotypes for agronomic traits, such as disease resistance, drought tolerance, and yield. Identifying the genetic loci or genes associated with these traits using GWAS or functional analyses will benefit future plant breeding efforts seeking to incorporate these traits into new crop varieties, whether through conventional breeding or gene editing techniques. Unmanned aerial vehicle (UAV)-based image data has been increasingly used for this task, as it allows entire test plots to be quickly and cost-effectively phenotyped. In my research, I have developed methods to improve the accuracy of machine learning …

Performance Fatigability And Neuromuscular Responses During And After One Set Of Forearm Flexion Muscle Actions To Failure At 30% Versus 70% One-Repetition Maximum In Men And Women, Tyler Joseph Neltner

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Previous studies have examined sex differences in performance fatigability during isometric, isokinetic and controlled dynamic constant external resistance (DCER) fatiguing tasks (FT), however, whether those sex differences remain during maximal intended velocity (MIV) DCER muscle actions remains unclear. The purpose of this study was to examine the sex-related differences in the time course of fatigue, neuromuscular responses, and coactivation from the ipsilateral and contralateral limbs during MIV DCER muscle actions to failure at low versus high relative loads, as well as performance fatigability from pre- to post-testing. Twenty-seven college-aged men (n = 14) and women (n = 13) …

A Collection Of Short Stories, Tryphena Lizzert Yeboah

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The short stories in this collection offer diverse and expansive realities of the human condition, particularly around the lives of women—their identities, bodies, emotions, and how they live and navigate a social order that is often constructed to dominate and undermine them. How can we complicate marginalized portrayals of women shaped by the male gaze and interrogate the patriarchal presumption of the African woman? What different approaches should be employed in capturing different aspects of her domestic and social life, how she defines and expresses autonomy, how she subverts gender norms, and her experiences of emotional and physical violence? These …

Growth And Simulation Of Dielectric-Metal Nanoheterostructured Thin Films By Ballistic Deposition, Shawn Wimer

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This thesis is divided into two related topics: the structural and chemical characterization of dielectric-metal nanoheterostructured thin films grown by glancing angle deposition (GLAD) and the results of the simulated growth of films and structures by GLAD with discrete-space and continuous-space Monte Carlo ballistic simulation.

Individual structures comprising films grown by GLAD were investigated by S/TEM and SEM imaging, electron diffraction, and chemical characterization and mapping by EDX. Different intermolecular interactions within and between different materials, including Si, Ag, Au, and ZrO₂, are linked to differences in the shapes of individual materials in heterostructures, the shape of heterostructures …

Laser 3d Printing Of Diamond-Based Composite Materials For Thermal Management Applications, Nada Kraiem

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

With the trend towards miniaturization of electrical equipment and the constant increase in power density in semiconductor devices, efficient heat management has become a major concern for researchers. Indeed, this technological evolution imposes increasingly strict constraints in terms of thermal dissipation, necessitating innovative solutions to ensure better durability and reliability of components. In this context, the use of composite materials offering high thermal conductivity (TC) and low coefficient of thermal expansion (CTE) compared to pure metals has become essential to address overheating issues in electronic components.

The utilization of advanced materials such as diamond (D), with exceptional TC and hardness …

Population Demography, Occupancy, And Connectivity Of Invasive Bigheaded Carp In The Platte River, Nebraska And Its Tributaries, Blake W. Logan

School of Natural Resources: Dissertations, Theses, and Student Research

Silver Carp Hypophthalmichthys molitrix and Bighead Carp H. nobilis, collectively referred to as bigheaded carp, are invasive species in the United States of America that have expanded their range throughout the Missouri River basin in the last three decades. It may be desirable to develop management plans directed at the monitoring, control, or removal of bigheaded carp within the Missouri River basin. However, a knowledge gap exists regarding bigheaded carp ecology in Missouri River tributaries, which may be important for the development of management plans. As such, this study characterized bigheaded carp population demographics, occupancy, and connectivity in …

Integrating Remote Sensing And Machine Learning To Determine Past, Current And Future Crop Water Use From The Nubian Sandstone Aquifer System, Moaz Ishag

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The agriculture sector is a significant consumer of water, and sustainable water use begins with monitoring irrigated land. Delineating irrigated land supports decision-makers and promotes the sustainable use of this crucial resource. This study focuses on the Nubian Sandstone Aquifer System (NSAS), the largest aquifers in the world, which spans Egypt, Sudan, Libya, and Chad. The study aims to: 1) quantify the increase in irrigated hectares (both pivot and non-pivot) from 2000-2001 to 2023-2024; 2) identify major irrigated crop types and their water requirements; and 3) quantify groundwater crop water use from the NSAS using remote sensing via the Google …

Structural Analysis Of Dj-1 Glyoxalase Activity By Mix-And-Inject Serial Synchrotron Crystallography, Coleman Dolamore

Department of Biochemistry: Dissertations, Theses, and Student Research

Observing enzyme structures while they are catalyzing a reaction has been a major goal of both enzymology and structural biology for decades. With the advent of time-resolved serial X-ray crystallography using X-ray free electron lasers (XFELs) and synchrotron sources, enzyme reactions can be monitored in real time in crystallo. Here, we use two different approaches to study related enzymes involved in methylglyoxal detoxification. For human DJ-1, we used pink beam mix-and-inject serial crystallography (MISC) at the Advanced Photon Source (BioCARS 14-ID) to probe the controversial mechanism of DJ-1’s action on methylglyoxal by using serial Laue diffraction. The high flux …

A Study Of Electron- And Photon-Induced Dissociative Electron Attachment To Molecules And Within Anion-Molecule Clusters, Mahmudul Hasan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Dissociative electron attachment (DEA) is a resonant process in which a molecule captures a low-energy electron, forming a transient negative ion (TNI). Subsequently, this unstable TNI fragments into a stable anion and one or more neutral fragments. DEA is crucial in various phenomena, ranging from atmospheric and radiation chemistry to processes occurring in plasmas, and is particularly significant in the context of radiation-induced damage to biological molecules. This study uses different experimental methods to better understand the fragmentation in molecular anions forming through dissociative photoexcitation and electron attachment. We have developed an experimental apparatus for dissociative photoexcitation studies. This apparatus …

Electronic Properties Of Group-Iii Nitride Semiconductors And Device Structures Probed By Thz Optical Hall, Nerijus Armakavicius, Philipp Kühne, Alexis Papamichail, Hengfang Zhang, Sean Knight, Axel Persson, Vallery Stanishev, Jr-Tai Chen, Plamen Paskov, Mathias Schubert, Vanya Darakchieva

Department of Electrical and Computer Engineering: Faculty Publications

Group-III nitrides have transformed solid-state lighting and are strategically positioned to revolutionize high-power and high-frequency electronics. To drive this development forward, a deep understanding of fundamental material properties, such as charge carrier behavior, is essential and can also unveil new and unforeseen applications. This underscores the necessity for novel characterization tools to study group-III nitride materials and devices. The optical Hall effect (OHE) emerges as a contactless method for exploring the transport and electronic properties of semiconductor materials, simultaneously offering insights into their dielectric function. This nondestructive technique employs spectroscopic ellipsometry at long wavelengths in the presence of a magnetic …

Exploring The Associations Of Afterschool Science Participation And Friendships With Science Identities, Patricia Wonch Hill, Grace M. Kelly, Julia Mcquillan, Jorge Ledesma, Miranda Melson, G. Robin Gauthier

Worlds of Connections Resources

Building on previous research that demonstrates the association of youth experiences in afterschool science and higher science identities, this paper presents a network study of 421 middle school students that examines afterschool science participation, friendship ties, and science identities. Participation in afterschool science clubs is associated with higher science identity, but the mechanisms and order of causality are unclear. Youth form friendships inside and outside of school, and peers may influence participation in afterschool activities, as empirical research on friendships shows that they are associated with youth interests. These peer interactions also have the potential to shape identity development during …

Evaluation Of An Autonomous Acoustic Surveying Technique For Grassland Bird Communities In Nebraska, Grace E. Schuster, Leroy J. Walston, Andrew R. Little

School of Natural Resources: Faculty Publications

Monitoring trends in wildlife communities is integral to making informed land management decisions and applying conservation strategies. Birds inhabit most niches in every environment and because of this they are widely accepted as an indicator species for environmental health. Traditionally, point counts are the common method to survey bird populations, however, passive acoustic monitoring approaches using autonomous recording units have been shown to be cost-effective alternatives to point count surveys. Advancements in automatic acoustic classification technologies, such as BirdNET, can aid in these efforts by quickly processing large volumes of acoustic recordings to identify bird species. While the utility of …

Daily Eicosapentaenoic Acid Infusion In Iugr Fetal Lambs Reduced Systemic Inflammation, Increased Muscle Adrβ2 Content, And Improved Myoblast Function And Muscle Growth, Haley Beer, Taylor A. Lacey, Rachel L. Gibbs, Micah S. Most, Zena M. Hicks, Pablo C. Grijalva, Eileen S. Marks-Nelson, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Intrauterine growth-restricted (IUGR) fetuses exhibit systemic inflammation that contributes to programmed deficits in myoblast function and muscle growth. Thus, we sought to determine if targeting fetal inflammation improves muscle growth outcomes. Heat stress-induced IUGR fetal lambs were infused with eicosapentaenoic acid (IUGR+EPA; n = 9) or saline (IUGR; n = 8) for 5 days during late gestation and compared to saline-infused controls (n = 11). Circulating eicosapentaenoic acid was 42% less (p < 0.05) for IUGR fetuses but was recovered in IUGR+EPA fetuses. The infusion did not improve placental function or fetal O2 but resolved the 67% greater (p < 0.05) circulating TNFα observed in IUGR fetuses. This improved myoblast function and muscle growth, as the 23% reduction (p < 0.05) in the ex vivo differentiation of IUGR myoblasts was resolved in IUGR+EPA myoblasts. Semitendinosus, longissimus dorsi, and flexor digitorum superficialis muscles were 24–39% lighter (p < 0.05) for IUGR but not for IUGR+EPA fetuses. Elevated (p < 0.05) IL6R and reduced (p < 0.05) β2 adrenoceptor content in IUGR muscle indicated enhanced inflammatory sensitivity and diminished β2 adrenergic sensitivity. Although IL6R remained elevated, β2 adrenoceptor deficits were resolved in IUGR+EPA muscle, demonstrating a unique underlying mechanism for muscle dysregulation. These findings show that fetal inflammation contributes to IUGR muscle growth deficits and thus may be an effective target for intervention.

Perspectives On Computational Modeling Of Biological Systems And The Significance Of The Sysmod Community, Bhanwar Lal Puniya, Meghna Verma, Chiara Damiani, Shaimaa Bakr, Andreas Dräger

Department of Biochemistry: Faculty Publications

Motivation: In recent years, applying computational modeling to systems biology has caused a substantial surge in both discovery and practical applications and a significant shift in our understanding of the complexity inherent in biological systems.

Results: In this perspective article, we briefly overview computational modeling in biology, highlighting recent advancements such as multiscale modeling due to the omics revolution, single-cell technology, and integration of artificial intelligence and machine learning approaches. We also discuss the primary challenges faced: integration, standardization, model complexity, scalability, and interdisciplinary collaboration. Lastly, we highlight the contribution made by the Computational Modeling of Biological Systems …

Sensitization Of Europium Oxide Nanoparticles Enhances Signal-To- Noise Over Autofluorescence With Time-Gated Luminescence Detection, Hunter Miller, Jessica Q. Wallace, Hui Li, Xing-Zhong Li, Ana De Bettencourt-Dias, Forrest Kievit

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Clinical translation of nanoparticle-based therapeutics has been limited, and a lack of preclinical delivery characterization is partly to blame, limiting our understanding of the mechanisms of failure. The improvement of the preclinical delivery assessment requires nanoparticles with higher detectability. This work focused on the exploration of several aromatic carboxylic ligands (terephthalic acid, quinaldic acid, and kynurenic acid) for the sensitization of europium oxide nanoparticles with a long emission lifetime to overcome cellular autofluorescence, a key confounder of detection in luminescence-based bioimaging. A facile one-pot synthesis and ligand exchange process generated and sensitized ultrasmall Eu₂O₃ cores. As reflected …

Computable Species Descriptions And Nanopublications: Applying Ontology-Based Technologies To Dung Beetles (Coleoptera, Scarabaeinae), Giulio Montanaro, James P. Balhoff, Jennifer C. Girón, Max Söderholm, Sergei Tarasov

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

Taxonomy has long struggled with analysing vast amounts of phenotypic data due to computational and accessibility challenges. Ontology-based technologies provide a framework for modelling semantic phenotypes that are understandable by computers and compliant with FAIR principles. In this paper, we explore the use of Phenoscript, an emerging language designed for creating semantic phenotypes, to produce computable species descriptions. Our case study centers on the application of this approach to dung beetles (Coleoptera, Scarabaeinae).