Digital Commons Network^™

Combining Corn N Recommendation Tools For An Improved Economical Optimal Nitrogen Rate Estimation, Curtis J. Ransom, Newell R. Kitchen, James J. Camberato, Paul R. Carter, Richard B. Ferguson, Fabián G. Fernández, David W. Franzen, Carrie A. M. Laboski, David Brenton Myers, Emerson D. Nafziger, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Improving corn (Zea mays L.) nitrogen (N) rate fertilizer recommendation tools can improve farmers’ profits and mitigate N pollution. Numerous approaches have been tested to improve these tools, but to date improvements for predicting economically optimum N rate (EONR) have been modest. This work’s objective was to use ensemble learning to improve our estimation of EONR (for a single at-planting and split N application timing) by combining multiple corn N recommendation tools. The evaluation was conducted using 49 corn N response trials from eight states in the US Corn Belt and three growing seasons (2014–2016). Elastic net and decision …

De Novo Assembly And Comparative Analyses Of Mitochondrial Genomes In Piperales, Runxian Yu, Xudong Chen, Lingjie Long, Matthias Jost, Ran Zhao, Lumei Liu, Jeffrey P. Mower, Claude W. Depamphilis, Stefan Wanke, Yuannian Jiao

Department of Agronomy and Horticulture: Faculty Publications

The mitochondrial genome of Liriodendron tulipifera exhibits many ancestral angiosperm features and a remarkably slow evolutionary rate, while mitochondrial genomes of other magnoliids remain yet to be characterized. We assembled nine new mitochondrial genomes, representing all genera of perianth-bearing Piperales, as well as for a member of the sister clade: three complete or nearly complete mitochondrial genomes from Aristolochiaceae and six additional draft assemblies including Thottea, Asaraceae, Lactoridaceae, and Hydnoraceae. For comparative purpose, a complete mitochondrial genome was assembled for Saururus, a member of the perianth-less Piperales. The average number of short repeats (50–99 bp) was much larger …

Azimuthal Correlations In Z +Jets Events In Proton–Proton Collisions At √S = 13tev, Cms Collaboration

Department of Physics and Astronomy: Faculty Publications

The production of Z bosons associated with jets is measured in pp collisions at √ s = 13 TeV with data recorded with the CMS experiment at the LHC corresponding to an integrated luminosity of 36.3 fb⁻¹. The multiplicity of jets with transverse momentum p_T > 30 GeV is measured for different regions of the Z boson’s p_T(Z ), from lower than 10 GeV to higher than 100 GeV. The azimuthal correlation Δφ between the Z boson and the leading jet, as well as the correlations between the two leading jets are measured in three regions …

An Older Diabetes-Induced Mice Model For Studying Skin Wound Healing, Carlos Poblete Jara, Guilherme Nogueira, Joseane Morari, Thaís Paulino Do Prado, Renan De Medeiros Bezerra, Lício A. Velloso, William Velander, Eliana Pereira De Araújo

Department of Chemical and Biomolecular Engineering: Faculty Publications

Advances in wound treatment depend on the availability of animal models that reflect key aspects of human wound healing physiology. To this date, the accepted mouse models do not reflect defects in the healing process for chronic wounds that are associated with type two diabetic skin ulcers. The long term, systemic physiologic stress that occurs in middle aged or older Type 2 diabetes patients is difficult to simulate in preclinical animal model. We have strived to incorporate the essential elements of this stress in a manageable mouse model: long term metabolic stress from obesity to include the effects of middle …

Gloria - A Globally Representative Hyperspectral In Situ Dataset For Optical Sensing Of Water Quality, Moritz K. Lehmann

School of Natural Resources: Faculty Publications

The development of algorithms for remote sensing of water quality (RSWQ) requires a large amount of in situ data to account for the bio-geo-optical diversity of inland and coastal waters. The GLObal Reflectance community dataset for Imaging and optical sensing of Aquatic environments (GLORIA) includes 7,572 curated hyperspectral remote sensing reflectance measurements at 1nm intervals within the 350 to 900nm wavelength range. In addition, at least one co-located water quality measurement of chlorophyll α, total suspended solids, absorption by dissolved substances, and Secchi depth, is provided. The data were contributed by researchers affiliated with 59 institutions worldwide and come from …

The Ecology Of Human-Caused Mortality For A Protected Large Carnivore, John F. Benson, Kyle D. Dougherty, Paul Beier, Walter M. Boyce, Bogdan Cristescu, Daniel J. Gammons, David K. Garcelon, J. Mark Higley, Quinton E. Martins, Anna C. Nisi, Seth P. D. Riley, Jeff A. Sikich, Thomas R. Stephenson, T. Winston Vickers, Greta M. Wengert, Christopher C. Wilmers, Heiko U. Wittmer, Justin A. Dellinger

School of Natural Resources: Faculty Publications

Mitigating human-caused mortality for large carnivores is a pressing global challenge for wildlife conservation. However, mortality is almost exclusively studied at local (within-population) scales creating a mismatch between our understanding of risk and the spatial extent most relevant to conservation and management of wide-ranging species. Here, we quantified mortality for 590 radio-collared mountain lions statewide across their distribution in California to identify drivers of human-caused mortality and investigate whether human-caused mortality is additive or compensatory. Human-caused mortality, primarily from conflict management and vehicles, exceeded natural mortality despite mountain lions being protected from hunting. Our data indicate that human-caused mortality is …

Search For The Higgs Boson Decay To A Pair Of Electrons In Proton-Proton Collisions At √S = 13 Tev, A. Tumasyan

Department of Physics and Astronomy: Faculty Publications

A search is presented for the Higgs boson decay to a pair of electrons (e⁺e⁻) in proton-proton collisions at √ s = 13 TeV. The data set was collected with the CMS experiment at the LHC between 2016 and 2018, corresponding to an integrated luminosity of 138 fb⁻¹. The analysis uses event categories targeting Higgs boson production via gluon fusion and vector boson fusion. The observed upper limit on the Higgs boson branching fraction to an electron pair is 3.0 × 10⁻⁴ (3.0 × 10⁻⁴ expected) at the 95% confidence level, which …

Advancing Ionomer Design To Boost Interfacial And Thin-Film Proton Conductivity Via Styrene-Calix[4]Arene-Based Ionomers, Shyambo Chatterjee, Oghenetega Allen Obewhere, Ehsan Zamani, Rajesh Keloth, Seefat Farzin, Martha D. Morton, Anandakumar Sarella, Shudipto Konika Dishari

Department of Chemical and Biomolecular Engineering: Faculty Publications

Sub-micrometer-thick ion-conducting polymer (ionomer) layers often suffer from poor ionic conductivity at the substrate/catalyst interface. The weak proton conductivity makes the electrochemical reaction at the cathode of proton-exchange-membrane fuel cells sluggish. To address this, here we report on a class of polystyrene-based ionomers having sub-nanometer-sized, sulfonated macrocyclic calix[4]arene-based pendants (PS-calix). In films with thickness comparable to that of ionomer-based binder layers, the conductivity of PS-calix film (∼41 mS/cm) is ∼13 times higher than that of the current state-of-the-art ionomer, Nafion. We observe a similar improvement in proton conductivity when PS-calix interfaces with Pt nanoparticles, demonstrating the potential of PS-calix in …

Erratum: Measurement Of Prompt And Nonprompt Charmonium Suppression In Pbpb Collisions At 5.02 Tev, A. Tumasyan

Department of Physics and Astronomy: Faculty Publications

In Fig. 3, the y axis titles were mistakenly written showing a single-differential cross section in either dimuon p_T or rapidity, when in fact the cross section is normalized by both the p_T and rapidity ranges used for a given measurement point. The corrected version is shown in the new Fig. 3 provided below.

Effects Of Intermixing And Oxygen Vacancies On A Two-Dimensional Electron Gas At The Polar (Tbsco3/Ktao) (001) Interface, Bhubnesh Lama, Evgeny Y. Tsymbal, Tula R. Paudel

Department of Physics and Astronomy: Faculty Publications

3d-5d perovskite oxides, ABO₃ (where A and B are 3d or 5d elements), form polar surfaces in the (001)- stacked thin films. As a result, the polar-polar (001) interface between two ABO₃ insulators could create polar discontinuity potentially producing a two-dimensional electron gas of higher density and stronger spatial localization compared to the widely studied polar-nonpolar oxide interfaces, such as (001) LaAlO₃/SrTiO₃. Here, as a model system, we explore the interface between polar (001) TbScO₃ and polar (001) KTaO₃ using first-principles density functional theory.We find that the …

Moving Beyond Fulfillment: Wisdom Years Stories Of Passion, Perseverance, And Productivity, Kenneth A. Kiewra, Jessica Walsh, Chris Labenz

Department of Educational Psychology: Faculty Publications

Seven participants were interviewed to uncover how they remain so productive in their wisdom years, those typically marked by retirement. Participants included a leading educational psychologist, a renowned national television news anchor, a four-time national champion collegiate coach, the founder and former chief executive of Arbor Day Foundation, a university scholar turned playwright, and two female adventurers who quit their jobs, sold their possessions, and have lived a nomadic life, hiking thousands of miles throughout America. Their wisdom years stories describe how and why they shun retirement and remain productive. The article concludes with seven advice-laden conclusions for readers: (a) …

A Monographic Revision Of The Jewel Scarabs Genus Chrysina From Panama, Colombia, And Ecuador (Coleoptera: Scarabaeidae: Rutelinae: Rutelini), Manuel D. Barria

Zea E-Books Collection

The work is a taxonomic revision of 28 species of the genus Chrysina Kirby (Scarabaeidae: Rutelinae: Rutelini) found in Panama (25), Colombia (2), and Ecuador (3). Chrysina tricolor (Ohaus), Chrysina chalcothea (Bates), and Chrysina cupreomarginata (F. Bates) are new re­cords for Panama. A new country record from northern Colombia is confirmed for Chrys­ina mercedesae Barria. Chrysina gaitalica Curoe and Hawks and Chrysina galbina Hawks are discovered at new localities in Panama; females of both species are discovered and described. Chrysina aurora (Bates) known from other localities in the west of the country, is rediscov­ered in Veraguas province 147 years after …

Drought Stress Prediction And Propagation Using Time Series Modeling On Multimodal Plant Image Sequences, Sruti Das Choudhury, Sinjoy Saha, Ashok Samal, Anastasios Mazis, Tala Awada

School of Natural Resources: Faculty Publications

The paper introduces two novel algorithms for predicting and propagating drought stress in plants using image sequences captured by cameras in two modalities, i.e., visible light and hyperspectral. The first algorithm, VisStressPredict, computes a time series of holistic phenotypes, e.g., height, biomass, and size, by analyzing image sequences captured by a visible light camera at discrete time intervals and then adapts dynamic time warping (DTW), a technique for measuring similarity between temporal sequences for dynamic phenotypic analysis, to predict the onset of drought stress. The second algorithm, HyperStressPropagateNet, leverages a deep neural network for temporal stress propagation using hyperspectral imagery. …

The Uncertain Fate Of L.B. 753 Under Article Vii, Section 11 Of The Nebraska Constitution’S No-Aid Provision, Anthony B. Schutz

Nebraska Law Bulletin (Selected Issues)

The Nebraska Legislature is considering a bill–Legislative Bill 753 (L.B. 753)–that would provide a non-refundable, 5-year carry-over tax credit for donations to qualifying scholarship-providing organizations. To qualify, the scholarship organization must provide qualifying education scholarships. Those qualifying education scholarships must be used to pay tuition and fees at a qualifying school. Qualifying schools are “any nongovernmental, privately operated elementary or secondary school located in this state.” The state administers the issuance of the tax credits through a program that keeps the budgeted caps in place. The program starts with a $25 million cap that gradually expands to $100 million if …

Periconceptual Maternal Nutrition Affects Fetal Liver Programming Of Energy- And Lipid-Related Genes, Wellison J. S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

During pregnancy, the fetus relies on the dam for its nutrient supply. Nutritional stimuli during fetal organ development can program hepatic metabolism and function. Herein, we investigated the role of vitamin and mineral supplementation (VTM or NoVTM—at least 71 days pre-breeding to day 83 of gestation) and rate of weight gain (low (LG) or moderate (MG)—from breeding to day 83) on the fetal liver transcriptome and the underlying biological pathways. Crossbred Angus beef heifers (n = 35) were randomly assigned to one of four treatments in a 2 × 2 factorial design (VTM_LG, VTM_MG, NoVTM_LG, and NoVTM_MG). Gene expression …

Expression Of Malic Enzyme Reveals Subcellular Carbon Partitioning For Storage Reserve Production In Soybeans, Usda Agricultural Research Service, Donald Danforth Plant Science Center, Donald Danforth Plant Science Center, Thomas E. Clemente, Hanh T. Nguyen

Department of Agronomy and Horticulture: Faculty Publications

• Central metabolism produces amino and fatty acids for protein and lipids that establish seed value. Biosynthesis of storage reserves occurs in multiple organelles that exchange central intermediates including two essential metabolites, malate, and pyruvate that are linked by malic enzyme. Malic enzyme can be active in multiple subcellular compartments, partitioning carbon and reducing equivalents for anabolic and catabolic requirements. Prior studies based on isotopic labeling and steady-state metabolic flux analyses indicated malic enzyme provides carbon for fatty acid biosynthesis in plants, though genetic evidence confirming this role is lacking. We hypothesized that increasing malic enzyme flux would alter carbon partitioning …

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Natural Variation In Host Feeding Behaviors Impacts Host Disease And Pathogen Transmission Potential, Alaina C. Pfenning-Butterworth, Rachel E. Vetter, Jessica L. Hite

School of Biological Sciences: Faculty Publications

Animals ranging from mosquitoes to humans often vary their feeding behavior when infected or merely exposed to pathogens. These so-called “sickness behaviors” are part of the innate immune response with many consequences, including avoiding orally transmitted pathogens. Fully understanding the role of this ubiquitous behavior in host defense and pathogen evolution requires a quantitative account of its impact on host and pathogen fitness across environmentally relevant contexts. Here, we use a zooplankton host and fungal pathogen as a case study to ask if infection-mediated feeding behaviors vary across pathogen exposure levels and natural genetic variation in susceptibility to infection. Then, …

Leaf-Counting In Monocot Plants Using Deep Regression Models, Xinyan Xie, Yufeng Ge, Harkamal Walia, Jinliang Yang, Hongfeng Yu

School of Computing: Faculty Publications

Leaf numbers are vital in estimating the yield of crops. Traditional manual leaf-counting is tedious, costly, and an enormous job. Recent convolutional neural network-based approaches achieve promising results for rosette plants. However, there is a lack of effective solutions to tackle leaf counting for monocot plants, such as sorghum and maize. The existing approaches often require substantial training datasets and annotations, thus incurring significant overheads for labeling. Moreover, these approaches can easily fail when leaf structures are occluded in images. To address these issues, we present a new deep neural network-based method that does not require any effort to label …

Rising Interest Rates: What They Mean For You And The Economy, Timothy Meyer

Cornhusker Economics

Discusses rising interest rates what they mean for agricultural workers and the economy.

A Comparison Of Task-Based And Questionnaire Assessments Of Executive Control Aspects In Relation To Adolescent Marijuana Initiation, Ying Guo, Charles B. Fleming, Irina Patwardhan, Tiffany D. James, Jennifer Nelson, Kimberly A. Espy, Timothy D. Nelson, W. Alex Mason

Department of Psychology: Faculty Publications

Prior research has linked deficits in executive control (EC) to marijuana use in adolescents but has relied either primarily on adolescent self-report of EC or tasked-based EC, and focused on limited aspects of EC, usually inhibitory control. We examined unique associations of three established aspects of EC (inhibitory control, working memory, and flexible shifting) assessed with both performance on laboratory tasks and self-report in relation to marijuana initiation. Participants were 260 youth (ages 14–18 years) from a small Midwestern city in the United States enrolled in the adolescent phase (beginning in 2017) of an ongoing study of EC development recruited …

Population Dynamics Of A Long-Term Selection Experiment In White Plymouth Rock Chickens Selected For Low Or High Body Weight, S. J. Harrison, P. B. Siegel, C. F. Honaker, Ronald M. Lewis

Department of Animal Science: Faculty Publications

The population dynamics of 2 lines of chickens from a long-term (59 generations) selection experiment were assessed based on pedigree data. These lines were propagated from phenotypic selection for low and high 8-wk BW in White Plymouth Rock chickens. Our objective was to determine whether the 2 lines maintained similar population structures over the selection horizon to allow meaningful comparisons of their performance data. A complete pedigree of 31,909 individuals, consisting of 102 founders, 1,064 from the parental generation, and 16,245 low weight (LWS) and 14,498 high weight (HWS) select chickens, was available. Inbreeding (F …

Additional Taxonomic Refinements Suggested By Genomic Analysis Of Butterflies, Jing Zhang, Qian Cong, Jinhui Shen, Leina Song, Paul A. Opler, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Comparative analyses of genomic data reveal further insights into the phylogeny and taxonomic classification of butterflies presented here. As a result, 2 new subgenera and 2 new species of Hesperiidae are described: Borna Grishin, subgen. n. (type species Godmania borincona Watson, 1937) and Lilla Grishin, subgen. n. (type species Choranthus lilliae Bell, 1931) of Choranthus Scudder, 1872, Cecropterus (Murgaria) markwalkeri Grishin, sp. n. (type locality in Mexico: Sonora), and Hedone yunga Grishin, sp. n. (type locality in Bolivia: Yungas, La Paz). The lectotype is designated for Aethilla toxeus Plötz, 1882. The type locality of Dion uza (Hewitson, 1877) is likely …

New Synonyms And Records Of Costa Rican And Panamanian Dung Beetles (Coleoptera: Scarabaeidae: Scarabaeinae), Ángel Solis, Bert Kohlmann

Insecta Mundi

To better understand the dung beetle (Coleoptera: Scarabaeidae: Scarabaeinae) biodiversity of Costa Rica and Panama, new synonyms, records, distributions, and updates are presented. This paper analyzes the distribution and taxonomy of Phanaeus olsoufieffi Balthasar, 1939 in Panama and establishes the following new subjective synonym: Phanaeus panamensis Moctezuma and Halffter, 2021 = Phanaeus olsoufieffi Balthasar, 1939. Color morphs of Phanaeus pyrois Bates, 1887 in Costa Rica are analyzed. The Costa Rican distribution of Onthophagus bidentatus Drapiez, 1819 and O. marginicollis Harold, 1880 is stud­ied. Onthophagus bidentatus is recorded for Costa Rica for the first time. A population analysis of barcode mtDNA, …

Eeg Decoding Of Finger Numeral Configurations With Machine Learning, Roya Salehzadeh, Brian Rivera, Kaiwen Man, Nader Jalili, Firat Soylu

Department of Psychology: Faculty Publications

In this study, we used multivariate decoding methods to study processing differences between canonical (montring and count) and noncanonical finger numeral configurations (FNCs). While previous research investigated these processing differences using behavioral and event-related potentials (ERP) methods, conventional univariate ERP analyses focus on specific time intervals and electrode sites and fail to capture broader scalp distribution and EEG frequency patterns. To address this issue a supervised learning classifier—support vector machines (SVM)—was used to decode ERP scalp distributions and alpha-band power for montring, counting, and noncanonical FNCs (for integers 1 to 4). The SVM was used to test whether the numerical …

Emergenet: A Novel Deep-Learning Based Ensemble Segmentation Model For Emergence Timing Detection Of Coleoptile, Aankit Das, Sruti Das Choudhury, Amit Kumar Das, Ashok Samal, Tala Awada

School of Computing: Faculty Publications

The emergence timing of a plant, i.e., the time at which the plant is first visible from the surface of the soil, is an important phenotypic event and is an indicator of the successful establishment and growth of a plant. The paper introduces a novel deep-learning based model called EmergeNet with a customized loss function that adapts to plant growth for coleoptile (a rigid plant tissue that encloses the first leaves of a seedling) emergence timing detection. It can also track its growth from a time-lapse sequence of images with cluttered backgrounds and extreme variations in illumination. EmergeNet is a …

Endogenous L- To D-Amino Acid Residue Isomerization Modulates Selectivity Between Distinct Neuropeptide Receptor Family Members, Baba M. Yussif, Cole V. Blasing, James W. Checco

Department of Biochemistry: Faculty Publications

The L- to D-amino acid residue isomerization of neuropeptides is an understudied post-translational modification found in animals across several phyla. Despite its physiological importance, little information is available regarding the impact of endogenous peptide isomerization on receptor recognition and activation. As a result, the full roles peptide isomerization play in biology are not well understood. Here, we identify that the Aplysia allatotropin-related peptide (ATRP) signaling system utilizes L- to D-residue isomerization of one amino acid residue in the neuropeptide ligand to modulate selectivity between two distinct G protein-coupled receptors (GPCRs). We first identified a novel receptor for ATRP that is …

Evaluation Of Gross Energy Concentration Of Neutral Detergent Fiber Contained In Feed And Fecal Samples, J. D. Stypinski, Paul J. Kononoff, W. P. Weiss

Department of Animal Science: Faculty Publications

Starch and NDF are usually assumed to contain the same concentration of gross energy (GE), but NDF is more variable in chemical composition and varies more in the extent of digestion. The variable chemical composition of NDF may have direct implications on dairy nutrition models that predict dietary GE and use this estimate for also predicting digestible energy. For example, when NDF is enriched in lignin and protein, the concentration of GE would increase, whereas NDF enriched in ash would have the opposite effect. Current nutritional models, such as the NASEM (2021) and CNCPS (6.55), assume a GE coefficient of …

Intraguild Predation Is Increased In Areas Of Low Prey Diversity In A Generalist Predator Community, Stella F. Uiterwaal, Amber J. Squires, Bennett A. Grappone, Brian Dillard, Ariadne Castaneda, Sora L. Kim, John P. Delong

School of Biological Sciences: Faculty Publications

1. Niche differentiation and intraguild predation (IGP) can allow ecologically similar species to coexist, although it is unclear which coexistence mechanism predominates in consumer communities. Until now, a limited ability to quantify diets from metabarcoding data has precluded the use of sequencing data to determine the relative importance of these mechanisms.

2. Here, we pair a recent metabarcoding quantification approach with stable isotope analysis to examine diet composition in a wolf spider community.

3. We compare the prevalence of resource partitioning and IGP in these spiders and test whether factors that influence foraging performance, including individual identity, morphology, prey community and environmental conditions, …

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The Waxy Mutation In Sorghum And Other Cereal Grains Reshapes The Gut Microbiome By Reducing Levels Of Multiple Beneficial Species, Qinnan Yang, Mallory J. Suhr Van Haute, Scott E. Sattler, Devin J. Rose, Anthony Juritsch, Jing Shao, Kristin A. Beede, Robert J. Schmaltz, Jeff Price, John Toy, Amanda E. Ramer-Tait, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Waxy starches from cereal grains contain >90% amylopectin due to naturally occurring mutations that block amylose biosynthesis. Waxy starches have unique organoleptic characteristics (e.g. sticky rice) as well as desirable physicochemical properties for food processing. Using isogenic pairs of wild type sorghum lines and their waxy derivatives, we studied the effects of waxy starches in the whole grain context on the human gut microbiome. In vitro fermentations with human stool microbiomes show that beneficial taxonomic and metabolic signatures driven by grain from wild type parental lines are lost in fermentations of grain from the waxy derivatives and the beneficial signatures …

Optimal Stomatal Theory Predicts Co2 Responses Of Stomatal Conductance In Both Gymnosperm And Angiosperm Trees, Anna Gardner, Mingkai Jiang, David S. Ellsworth, A. Robert Mackenzie, Jeremy Pritchard, Martin Karl Friedrich Bader, Craig V.M. Barton, Carl Bernacchi, Carlo Calfapietra, Kristine Y. Crous, Mirindi Eric Dusenge, Teresa E. Gimeno, Marianne Hall, Shubhangi Lamba, Sebastian Leuzinger, Johan Uddling, Jeffrey Warren, Göran Wallin, Belinda E. Medlyn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Optimal stomatal theory predicts that stomata operate to maximise photosynthesis (Anet) and minimise transpirational water loss to achieve optimal intrinsic water-use efficiency (iWUE). We tested whether this theory can predict stomatal responses to elevated atmospheric CO2 (eCO2), and whether it can capture differences in responsiveness among woody plant functional types (PFTs). We conducted a meta-analysis of tree studies of the effect of eCO2 on iWUE and its components Anet and stomatal conductance (gs). We compared three PFTs, using the unified stomatal optimisation (USO) model to account for confounding effects of leaf–air vapour pressure difference (D). We expected smaller gs, but …