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Development And Validation Of High-Resolution Melting Assays For The Detection Of Potentially Virulent Strains Of Escherichia Coli O103 And O121, Frank J. Velez, Joseph M. Bosilevac, Sabine Delannoy, Patrick Fach, Ravinder Nagpal, Prashant Singh May 2022

Development And Validation Of High-Resolution Melting Assays For The Detection Of Potentially Virulent Strains Of Escherichia Coli O103 And O121, Frank J. Velez, Joseph M. Bosilevac, Sabine Delannoy, Patrick Fach, Ravinder Nagpal, Prashant Singh

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Virulent strains of Shiga toxin-producing Escherichia coli (STEC) serogroups O103 and O121 are considered adulterants in beef. Two high-resolution melting (HRM) real-time PCR assays were standardized for the specific detection and discrimination of potentially virulent and avirulent strains of E. coli O103 and O121. The O103 HRM assay offered the possibility to distinguish clearly STEC O103:H2 from STEC O103:H25. The two standardized assays were extensively validated using 215 pure culture strains, laboratory inoculated food samples, and naturally contaminated beef (n = 84) and pork (n = 84) enrichments collected from the red meat surveillance program. Both HRM assays showed 100% …


North Central Extension Risk Management Education Center Helps Producers Manage Risks, Christine Lockert, Bradley D. Lubben, Sheila Aikanathan Johnson May 2022

North Central Extension Risk Management Education Center Helps Producers Manage Risks, Christine Lockert, Bradley D. Lubben, Sheila Aikanathan Johnson

Cornhusker Economics

Farmers and ranchers face many risks and challenges in production agriculture every day. Changing market fundamentals, farm policy reforms, and crop insurance program developments defined the risk environment that led to the authorization of agricultural risk management education as part of the Agricultural Risk Protection Act of 2000. The educational efforts that have followed have been designed to help producers manage the full range of production, marketing, financial, legal, and human risks that are all part of agriculture. In the more than two decades since, the risks facing producers have continued to grow. Producers managed through both the opportunities of …


Covid‑Specific Coercive Control Among Emerging Adults Attending College: A Brief Note, Heather Littleton, Katie Edwards, Kayla E. Sall, Stephanie Lim, Victoria Mauer May 2022

Covid‑Specific Coercive Control Among Emerging Adults Attending College: A Brief Note, Heather Littleton, Katie Edwards, Kayla E. Sall, Stephanie Lim, Victoria Mauer

Department of Psychology: Faculty Publications

The COVID-19 pandemic represents a “perfect storm” with regards to risk for intimate partner violence (IPV). Abusive partners may engage in novel forms of coercive control, such as pressuring their partner to engage in activities associated with COVID-19 infection risk (e.g., attend a large gathering). However, no empirical research has focused on COVIDspecific coercive control. The current study sought to evaluate the prevalence of COVID-specific coercive control in a large sample of U.S. college students, as well as its association with other forms of IPV and depression and anxiety. A total of 2,289 undergraduate students attending eight U.S. universities who …


Development Of A Data-Assimilation System To Forecast Agricultural Systems: A Case Study Of Constraining Soil Water And Soil Nitrogen Dynamics In The Apsim Model, Marissa S. Kivi, Bethany Blakely, Michael Masters, Carl J. Bernacchi, Fernando E. Miguez, Hamze Dokoohaki May 2022

Development Of A Data-Assimilation System To Forecast Agricultural Systems: A Case Study Of Constraining Soil Water And Soil Nitrogen Dynamics In The Apsim Model, Marissa S. Kivi, Bethany Blakely, Michael Masters, Carl J. Bernacchi, Fernando E. Miguez, Hamze Dokoohaki

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

As we face today's large-scale agricultural issues, the need for robust methods of agricultural forecasting has never been clearer. Yet, the accuracy and precision of our forecasts remains limited by current tools and methods. To overcome the limitations of process-based models and observed data, we iteratively designed and tested a generalizable and robust data-assimilation system that systematically constrains state variables in the APSIM model to improve forecast accuracy and precision. Our final novel system utilizes the Ensemble Kalman Filter to constrain model states and update model parameters at observed time steps and incorporates an algorithm that improves system performance through …


Reinventing Educational Spaces, Angel Coleman May 2022

Reinventing Educational Spaces, Angel Coleman

Masters in Architecture Program: Theses

Problems always existed in our educational system, but many issues intensified in the pandemic. One of the biggest is the mental impact on the children, with those in high school tripling in numbers. The spaces these students spend their time has a great impact on their mental well-being. Our schools need to create places for the students to make communities and feel a sense of ownership. Giving students a place to belong and making them proud of their work helps build their self-esteem, relieving some mental health issues.

My thesis is redesigning these environments to create classrooms, schools and a …


Analysis Of The Effectiveness Of Different Techniques For Creating Cross-Platform Compatible Software, Michael Westberg May 2022

Analysis Of The Effectiveness Of Different Techniques For Creating Cross-Platform Compatible Software, Michael Westberg

Honors Program: Senior Projects (Public)

Creating cross-platform compatible software is a major issue in a world where users utilize a variety of devices and platforms. To ensure that a piece of software is accessible to as many users as possible, software must be cross-platform compatible. There are four main approaches that can be done to achieve this state of being cross-platform compatible, each with both advantages and disadvantages. These methods are: creating the software as separate binaries, using a scripting language with a cross-platform interpreter, compiling to an intermediate language, and creating the software as a web application. This paper will discuss how each of …


A Strategic Audit Of Seaworld, Thomas Wolfe May 2022

A Strategic Audit Of Seaworld, Thomas Wolfe

Honors Program: Senior Projects (Public)

A strategic audit of SeaWorld


Data Management In Web Applications To Balance Performance And Security, Caleb Marcoux May 2022

Data Management In Web Applications To Balance Performance And Security, Caleb Marcoux

Honors Program: Senior Projects (Public)

As web applications become increasingly popular, many are running several calculations and data processing on the client machine, it is important to consider data management practices on the front-end of these web applications. Typically, some data from the server is stored in the client's memory or hard disk. How much data should be stored for how long, as well as many other considerations, influence the time and space performance of the web application, as well as its security. In this thesis, we explore several challenges, solutions, and design patterns in web application data management through the lens of a senior …


Can Technology Reduce The Stress Of The Child Welfare Practitioner?, Quality Improvement Center For Workforce Development May 2022

Can Technology Reduce The Stress Of The Child Welfare Practitioner?, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Information and communication technology (ICT) “has the potential to dramatically shift and enhance social work practice,” according to Berzin, Singer, & Chan (2015). ICT includes tools that allow users to both communicate with colleagues and clients and access, store, transmit, and manipulate information (Perron et al., 2010). Such technology integration can create practices that are more flexible, on-demand, and individualized not only to the families served, but also to the practitioner. Mobile technology, as well as other technologies (e.g., gaming, social media, robotics, wearable technologies) will enhance practice gains and result in more timely, accurate, and targeted services (Berzin, Singer, …


Dr. Barbara Higdon: Let It Begin With Me, E. B. Bachman May 2022

Dr. Barbara Higdon: Let It Begin With Me, E. B. Bachman

Journal of Women in Educational Leadership

College and university leadership has been analyzed in many ways using critiques of leadership styles, reviews of issues and individual philosophies. An examination of Dr. Barbara Higdon’s career and impact as an educator and educational leader will add to the story of women educational leaders in the United States. The record of such would be incomplete without her story.


A Strategic Audit Of Seaworld, Jaden Capaldi, Rachel Jendro, Jacob Mir, Sophie Newman, Tommy Wolfe May 2022

A Strategic Audit Of Seaworld, Jaden Capaldi, Rachel Jendro, Jacob Mir, Sophie Newman, Tommy Wolfe

Honors Program: Senior Projects (Public)

No abstract provided.


Coinbase Global Inc. Strategic Audit, Decker Bobin May 2022

Coinbase Global Inc. Strategic Audit, Decker Bobin

Honors Program: Senior Projects (Public)

Coinbase is a cryptocurrency exchange founded in the U.S during 2012 by Brian Armstrong and Fred Ehrsam. Coinbase’s application allows users to buy, sell, and exchange different cryptocurrencies on the open market. Cryptocurrencies are digital assets used as a medium to quickly transfer value, globally, without any middleman. Some cryptocurrencies have different technology than others, but all are based on blockchain, and cryptography. Blockchain technology allows transactions to be built into a chain that is forever accessible, hence the name “public ledger.” Cryptography is what ensures ultimate ownership, through seed phrases, and security through encryption. Coinbase has several large competitors …


Effects Of Victimization And Community Characteristics On Health Outcomes, Katie Meyer May 2022

Effects Of Victimization And Community Characteristics On Health Outcomes, Katie Meyer

Department of Sociology: Dissertations, Theses, and Student Research

Drawing on the neighborhood disorder model, the environmental stress model, and general strain theory, this study examined the effects of victimization experiences, anticipated victimization, and perceived community characteristics on overall physical health and mental health outcomes. This study used cross-sectional survey data from the 2014-2015 Nebraska Annual Social Indicator Survey (NASIS). Linear regression was used to examine how victimization experiences, worry about victimization, community context, and perceptions of crime and policing were associated with the health outcomes, controlling for demographic characteristics. The findings show that more worry about crime, less positive community perceptions, and less perceived police protection were associated …


Brick Collage, Dehmie Dehmlow May 2022

Brick Collage, Dehmie Dehmlow

School of Art, Art History, and Design: Theses and Student Creative Work

bricolage: construction or creation from a diverse range of available things

I create abstracted modular sculptures, assemblages, and collages that playfully reference utility, using salvaged materials and carefully fabricated objects. My sculptures are considerately composed, elevating the materials with a determined focus on how each disparate part connects to the next to become a meaningful whole. I have a reverence for all of the objects and materials I use, no matter their origin, and thoroughly consider how each of their forms, textures, colors, weights and other formal and physical qualities integrate into a whole. With the use of recognizable utilitarian …


Nebline, May 2022 May 2022

Nebline, May 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Insect and Disease Control for Organic Vegetable Gardeners by Sarah Browning

Garden Guide: Things to Do This Month

An Egg-cellent Choice!

Recipe of the Month: Sweet Potatoes and Black Bean Hash by Alyssa Havlovic

It’s Time for Private Well Maintenance by Becky Scheurman

Cash Rent Survey

Composting Demonstrations

Monarchs, Migration & Milkweed by Kait Chapman

Getting Outside to Play by Hayley Jackson

4th Graders Learn About “Farm to Fork” at Ag Festival

4-H Clubs Helped at Kiwanis Karnival

Support Local 4-H Youth During “Give to Lincoln Day”

4-H Achievement Celebration

Heart of 4-H Volunteer Award: Rick Waldron

3 Lancaster County …


The Nebraska Covid-19 Early Care And Education Provider Survey Iii: “Holding It Together—And Hanging By A Thread” May 2022, Alexandra Daro, Kathleen C. Gallagher, Kristen M. Cunningham May 2022

The Nebraska Covid-19 Early Care And Education Provider Survey Iii: “Holding It Together—And Hanging By A Thread” May 2022, Alexandra Daro, Kathleen C. Gallagher, Kristen M. Cunningham

Buffet Early Childhood Institute Reports and Publications

In February 2022, the Buffett Early Childhood Institute conducted the Nebraska COVID-19 Early Care and Education Survey III, in collaboration with state agencies, University of Nebraska faculty, and organization partners. This third survey examined the impact of the COVID-19 pandemic on Nebraska’s child care professionals and its implications for practice and policy. Results from the previous surveys, released in April and August 2020, indicated that early care and education professionals in Nebraska, who were already vulnerable prior to the pandemic, have been negatively impacted from the start of the pandemic. The first survey elevated providers’ immediate needs, including funding relief, …


Inter-Cell Slicing Resource Partitioning Via Coordinated Multi-Agent Deep Reinforcement Learning, Tianlun Hu, Qi Liao, Qiang Liu, Dan Wellington, Georg Carle May 2022

Inter-Cell Slicing Resource Partitioning Via Coordinated Multi-Agent Deep Reinforcement Learning, Tianlun Hu, Qi Liao, Qiang Liu, Dan Wellington, Georg Carle

School of Computing: Faculty Publications

Network slicing enables the operator to configure virtual network instances for diverse services with specific requirements. To achieve the slice-aware radio resource scheduling, dynamic slicing resource partitioning is needed to orchestrate multi-cell slice resources and mitigate inter-cell interference. It is, however, challenging to derive the analytical solutions due to the complex inter-cell interdependencies, interslice resource constraints, and service-specific requirements. In this paper, we propose a multi-agent deep reinforcement learning (DRL) approach that improves the max-min slice performance while maintaining the constraints of resource capacity. We design two coordination schemes to allow distributed agents to coordinate and mitigate inter-cell interference. The …


Dontrell, Who Kissed The Sea: An Exploration Of Scenic Design Process, Emma Laine Hoffbauer May 2022

Dontrell, Who Kissed The Sea: An Exploration Of Scenic Design Process, Emma Laine Hoffbauer

Johnny Carson School of Theatre and Film: Theses, Student Research, and Creative Work

The purpose of this thesis is to provide research, supporting paperwork, production photographs, and other pertinent materials which document the scenic design for the University of Nebraska-Lincoln/Nebraska Repertory Theatre production of Dontrell, Who Kissed the Sea by Nathan Alan Davis. This thesis contains the following: research images collected to develop and communicate ideas regarding location, shape, texture, scale, mood, and historical and architectural reference to the production team; preliminary sketches and digital renderings, and photographs of the 1⁄4” scale model; a full set of drafting plates and painter’s elevations used to communicate finished appearance of each scenic element, a list …


Dontrell, Who Kissed The Sea: An Expedition Into Technical Direction, Joseph J. Shelly Jr May 2022

Dontrell, Who Kissed The Sea: An Expedition Into Technical Direction, Joseph J. Shelly Jr

Johnny Carson School of Theatre and Film: Theses, Student Research, and Creative Work

The Nebraska Repertory Theatre, in partnership with the Johnny Carson School of Theater, Film, and Emerging Media Arts at the University of Nebraska – Lincoln, chose to produce Dontrell, Who Kissed the Sea in March of 2021. Playwright Nathan Alan Davis completed the play in 2017. He is originally from Rockford, Illinois, and a graduate of Indiana University. Dontrell was his second produced play, premiering in June 2014 at the Source Festival in Washington, DC. Several theatres since 2014 have produced the play, and ours was the third show of the 2021-2022 season. The director was Ron Himes, the Founder …


Symbolic Ns-3 For Efficient Exhaustive Testing, Jianfei Shao May 2022

Symbolic Ns-3 For Efficient Exhaustive Testing, Jianfei Shao

School of Computing: Dissertations, Theses, and Student Research

Exhaustive testing is an important type of simulation, where a user exhaustively simulates a protocol for all possible cases with respect to some uncertain factors, such as all possible packet delays or packet headers. It is useful for completely evaluating the protocol performance, finding the worst-case performance, and detecting possible design or implementation bugs of a protocol. It is, however, time consuming to use the brute force method with current NS-3, a widely used network simulator, for exhaustive testing. In this paper, we present our work on Sym-NS-3 for more efficient exhaustive testing, which leverages a powerful program analysis technique …


The Effects Of Human Activity On Reintroduced Bighorn Sheep (Ovis Canadensis) Populations, Justine Cherovsky May 2022

The Effects Of Human Activity On Reintroduced Bighorn Sheep (Ovis Canadensis) Populations, Justine Cherovsky

Department of Environmental Studies: Undergraduate Student Theses

Since the decimation of North America’s bighorn sheep (Ovis canadensis) populations due to human impacts, translocations have been the primary conservation strategy to repopulate the native habitat. Following the extirpation of the species in Nebraska, bighorns were reintroduced with the hope to establish a stable population, but nearly four decades of management has resulted in large-scale die-offs and unstable herds. Ongoing human impacts have created similar results across the country as reintroductions struggle to establish independent bighorn herds. A systematic literature review was conducted to analyze current human related effects, evaluate the effectiveness of management practices, and compare these findings …


University Of Nebraska Sustainability Recommendations, Kat Woerner May 2022

University Of Nebraska Sustainability Recommendations, Kat Woerner

Department of Environmental Studies: Undergraduate Student Theses

In August 2020, the University of Nebraska (NU) system published a 5-year strategic plan with a point to “set and achieve University-wide sustainability goals.” Sustainability efforts at the University of Nebraska are well established as each campus has developed a Sustainability Master Plan (SMP) over the course of the last six years. With limited resources, a system-wide, coordinated effort is critical and requires a hierarchy of actions that are based on: clear goals and metrics that support the university mission, effective tracking systems, policies, strategies, actions, practices that support the goals, and organizational structure to oversee and support implementation.

The …


How Can Stakeholders In Food Sovereignty Achieve More Political Power?, Micah Dierks May 2022

How Can Stakeholders In Food Sovereignty Achieve More Political Power?, Micah Dierks

Department of Environmental Studies: Undergraduate Student Theses

Food sovereignty (FSv) is a peasant-driven, transnational movement that emerged in response to land-grabbing and the consolidation of power in food production. It advocates for the rights of people to define their own food practices and systems. To provide targeted feedback for movement reflexivity, 21 ecological conflicts referencing food sovereignty were sampled from the Global Atlas of Environmental Justice (the EJAtlas). Involved groups of actors and their forms of mobilization were correlated with interpretations of success from the EJAtlas. I recommend that stakeholders in food sovereignty pursue the acquisition of greater political power through improving messaging, leveraging or disrupting existing …


"Folk" Home-Lore: Storytelling And The Architecture Of The Home, Madeleine Pollara May 2022

"Folk" Home-Lore: Storytelling And The Architecture Of The Home, Madeleine Pollara

Masters in Architecture Program: Theses

I have observed that architecture is not sufficiently understood. This problem is largely due to the fact that the language used to describe architecture is broad and convoluted. This language is also divided into two categories which describe the denotative and connotative nature of architecture. It is easier to understand the denotative nature of architecture than it is to understand the connotative nature. As a response to this issue, my project aims to explore the connotative nature of architecture through telling stories about the home from the architecture’s point of view. The purpose of my thesis is to explore the …


Risk Assessment Of Hazardous Material Transportation For Small And Tribal Communities, Harrison Redepenning May 2022

Risk Assessment Of Hazardous Material Transportation For Small And Tribal Communities, Harrison Redepenning

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

By and large transportation of hazardous materials (HazMat) across the US is increasing, with truck transportation as the most common method of transport. Smaller communities (population <5,000) and Native American communities often lack the economic and political influence to quantify the dangers that HazMat incidents present to the immediate and surrounding areas. Lack of such information is an impediment to adequate preparedness in cases of HazMat incidents. This research focuses on assessing the vulnerability of small and tribal communities in Nebraska to the impacts of highway HazMat incidents. This was done by estimating the expected number and type of HazMat incidents per population in each community. Additionally, statistical analysis was conducted on actual HazMat incidents to determine if small and Native American communities experience a higher rate of incidents per population and per HazMat VMT than large and other small communities respectively. For per population, it was found small versus large is statistically significant while Native American versus other small communities was not significant. For per HazMat VMT, neither comparison was found to be statistically significant. In estimated HazMat incidents, actual HazMat incidents per population, and actual HazMat incidents per HazMat VMT, small communities had higher mean values than their large counterparts. Communities on Native American reservation land experienced a higher estimated and lower actual HazMat incident rate per population than other small communities. For actual HazMat incidents per VMT, Native American communities had a higher rate than other small communities.

Advisor: Aemal Khattak


It Takes Brains: Cultivating The Learning Process For Effective Science Communication, Amy D. Hauver May 2022

It Takes Brains: Cultivating The Learning Process For Effective Science Communication, Amy D. Hauver

Doctor of Plant Health Program: Dissertations and Student Research

Learning is a complex, subjective process. An important perspective on learning is that anyone, regardless of their level of education, can participate in learning about science and contribute to their community. The public increasingly looks towards online resources to find answers to challenges, so it is necessary that people become empowered to take information about issues rooted in science and apply them to their own lives and communities. In my experiences as a learner and educator, understanding the learning process provides a framework to design successful learning environments.

Since the brain is the organ most closely associated with the process …


First Line Of Defense: Role Of Surface Waxes In Sorghum Defense Against Aphids, Juan David Betancurt Cardona May 2022

First Line Of Defense: Role Of Surface Waxes In Sorghum Defense Against Aphids, Juan David Betancurt Cardona

Department of Entomology: Dissertations, Theses, and Student Research

Since 2013, the sugarcane aphid (SCA; Melanaphis sacchari) has become a devastating pest in sorghum (Sorghum bicolor) in the United States. Efforts to mitigate this pest and contain its rapid population growth have increased recently. Host plant resistance (HPR) is an effective and environmentally friendly strategy to reduce aphid populations in this crop. Many factors make SCA a challenging pest to control. For example, SCA can overcome drastic climatic changes, which allows them to successfully colonize sorghum plants at different developmental stages.

Epicuticular waxes (EW) constitute the first point of contact between plants and their environment. EW …


College Slasher Novel, Jeff Hill May 2022

College Slasher Novel, Jeff Hill

Department of English: Dissertations, Theses, and Student Research

This project was completed in hopes of creating a new novel that combines the research and craft worlds of composition and creative writing while merging the social worlds of teaching and campus Greek life, as well as making relevant contemporary commentary on the genres of satire and horror. In preparation, beyond necessary course work completion and time to outline, write, workshop, and revise, I read numerous novels and articles and watched dozens of films and television episodes as well as conducted research regarding current campus demographic to compose the best novel I could write in my time within the program. …


An Investigation Of The Attitudes And Behavioral Outcomes Of Nebraskan Hunters Toward Tick-Borne Disease, Dominic J. Cristiano May 2022

An Investigation Of The Attitudes And Behavioral Outcomes Of Nebraskan Hunters Toward Tick-Borne Disease, Dominic J. Cristiano

School of Natural Resources: Dissertations, Theses, and Student Research

As changes in climate, land-use, and vegetation alter the landscape of the Great Plains, new threats to public health are emerging. Incidences of tick-borne disease in Nebraska have increased nearly 250% over the past two decades – newly established species like Ixodes scapularis may introduce challenges for health practitioners, including more cases of Lyme disease. Strategies for tick-borne disease prevention must incorporate effective health messaging. Audience segmentation may be a useful technique to provide health communication, as it allows for targeted messaging that speaks to specific attitudes and beliefs of a given population. One tool for usefully segmenting populations is …


Feasibility Assessment On Use Of Proximal Geophysical Sensors To Support Precision Management, Sophia M. Becker May 2022

Feasibility Assessment On Use Of Proximal Geophysical Sensors To Support Precision Management, Sophia M. Becker

School of Natural Resources: Dissertations, Theses, and Student Research

Soil property maps provide information for field management activities such as irrigation, fertilization, and seeding. Many on-the-go proximal geophysical sensors have been developed in recent decades that can help map agricultural fields without dense soil sampling. To utilize these technologies most profitably in precision management, scientists and precision agriculture dealers must better understand sensors’ performances in given field conditions and the economic value of different proximal soil sensing methods. Chapter two reports the study that was conducted at three sites in North Dakota, United States to strengthen understanding of the usefulness of different proximal geophysical data types in agricultural contexts …