Digital Commons Network^™

Monitoring And Trends Of Antimicrobial Use In Livestock Production, Elliott James Dennis

Cornhusker Economics

There has been growing consumer and human medical profession concern about the use of “Shared Use/Medically Important” antimicrobials in food-animal production. The concern partially stems from the concern that livestock and poultry fed antimicrobials can develop resistant bacteria which can then be passed on to humans primarily via consumed meat and poultry products. To curtail this real or hypothetical consumer concern, major restaurants, food service companies, food processors, and supermarkets have pledged to reduce or eliminate meat and poultry that were raised with a non-negative quantity of antimicrobials. For example, Wendy’s© pledged that 50% of procured beef would come from …

Small Mammals Killed In Discarded Bottles Along Roadsides In Central Nebraska, Owen J. Johnson, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Littered debris along roadways traps and kills small vertebrates. In Nebraska, at least 20 species of small mammals are small enough to enter openings of discarded bottles and cans. We surveyed roadsides for littered bottles and cans containing vertebrate remains in central parts of the state. We observed 459 bottles and 278 aluminum cans along 17.6 km of roadsides in Nebraska. Littered bottles contained 41 vertebrate remains representing nine taxa of small mammals. Glass bottles contained the majority of individuals, plastic bottles had a few individuals, and no vertebrate remains were detected in aluminum cans. Harvest mice (Reithrodontomys spp.) …

Development Of A Preventive Influenza D Virus Vaccine, Qingsheng Li, Yanmin Wan, Feng Li

School of Biological Sciences: Faculty Publications

The present disclosure provides a DNA vaccine or immunogenic composition expressing consensus hemagglutinin esterase - fusion ( HEF ) protein ( FluD - Vax ) and a protein based vaccine utilizing the HEF consensus protein . Methods of making and using the compositions are also provided herein.

A Vernacular For Lincoln, Nebraska, Austin Riggins

Honors Program: Senior Projects (Public)

The contemporary vernacular architecture in the United States is a product of industrialization and globalization. One homogenous, mass produced vernacular has dominated nationwide and overshadowed the unique, contextual, and regional designs of the past. While the contemporary, industrialized vernacular has led to increases in the quality of life for many in the developed world, it has also left in its wake a homogenous and placeless environment devoid of environmental sensitivity or cultural references. There is a need for a set of new vernaculars that embrace modern building technologies while simultaneously responding more directly to local climatic needs and facilitating a …

Compounds Derived From Bacteria Enhance Marine Diatom Growth, Ananya Mukherjee

Center for Plant Science Innovation: Faculty Publications

No abstract provided.

Subsequent Spelling Ritchiei For Mnematium Ritchii Macleay, 1821 (Coleoptera: Scarabaeoidea: Scarabaeidae: Scarabaeini) To Be Maintained For Prevailing Usage, Stefano Ziani

Insecta Mundi

The specific name in Mnematium ritchiei Macleay, 1821 (Coleoptera: Scarabaeidae: Scarabaeini) is an incorrect subsequent spelling—the original spelling is ritchii—but, because of its prevailing usage here documented, is to be preserved.

A Catalogue Of The Cybocephalidae (Coleoptera: Cucujoidea) Of The World, Trevor Randall Smith

Insecta Mundi

A world catalogue and bibliography of the family Cybocephalidae (Coleoptera) is presented. The catalogue contains 207 species within 16 genera. Each taxon is accompanied by a complete taxonomic his­tory, including a full annotated synonymy with original references cited. The reversal of the recent synonymy of Cybocephalus brevis Grouvelle over Cybocephalus minimus Grouvelle is proposed. Cybocephalus aeneus Reiche is removed from synonymy with Cybocephalus micans Reitter and considered incertae sedis.

The Cybocephalidae are a family of small predatory beetles found throughout the world (Smith 2020). While cybocephalids are typically extremely convex, shiny and dark in coloration, the defining characteristic of …

Association Between Clinical Respiratory Signs, Lung Lesions Detected By Thoracic Ultrasonography And Growth Performance In Pre‐Weaned Dairy Calves, Inmaculada Cuevas-Gomez, Mark Mcgee, Jose Maria Sanchez, Edward O'Riordan, Nicky Byrne, Tara Mcdaneld, Bernadette Earley

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Background: Bovine respiratory disease (BRD) is the main cause of mortality among 1-to-5 month old calves in Ireland, accounting for approximately one-third of deaths. Despite widespread use of clinical respiratory signs for diagnosing BRD, lung lesions are detected, using thoracic ultrasonography (TUS) or following post-mortem, in calves showing no clinical signs. This highlights the limitation of clinical respiratory signs as a method of detecting sub-clinical BRD. Using 53 purchased artificially-reared male dairy calves, the objectives of this study were to: (i) characterise the BRD incidence detected by clinical respiratory signs and/or TUS, (ii) investigate the association between clinical respiratory signs …

Dataset Search In Biodiversity Research: Do Metadata In Data Repositories Reflect Scholarly Information Needs?, Felicitas Löffler, Valentin Wesp, Brigitta König-Ries, Friederike Klan

Copyright, Fair Use, Scholarly Communication, etc.

Abstract

The increasing amount of publicly available research data provides the opportunity to link and integrate data in order to create and prove novel hypotheses, to repeat experiments or to compare recent data to data collected at a different time or place. However, recent studies have shown that retrieving relevant data for data reuse is a time-consuming task in daily research practice. In this study, we explore what hampers dataset retrieval in biodiversity research, a field that produces a large amount of heterogeneous data. In particular, we focus on scholarly search interests and metadata, the primary source of data in …

Exploring Viirs Continuity With Modis In An Expedited Capability For Monitoring Drought-Related Vegetation Conditions, Trenton D. Benedict, Jesslyn F. Brown, Stephen P. Boyte, Daniel M. Howard, Brian A. Fuchs, Brian D. Wardlow, Tsegaye Tadesse, Kirk A. Evenson

National Drought Mitigation Center: Faculty Publications

Vegetation has been effectively monitored using remote sensing time-series vegetation index (VI) data for several decades. Drought monitoring has been a common application with algorithms tuned to capturing anomalous temporal and spatial vegetation patterns. Drought stress models, such as the Vegetation Drought Response Index (VegDRI), often use VIs like the Normalized Difference Vegetation Index (NDVI). The EROS expedited Moderate Resolution Imaging Spectrora-diometer (eMODIS)-based, 7-day NDVI composites are integral to the VegDRI. As MODIS satellite platforms (Terra and Aqua) approach mission end, the Visible Infrared Imaging Radiometer Suite (VIIRS) presents an alternate NDVI source, with daily collection, similar band passes, and …

Exploring Viirs Continuity With Modis In An Expedited Capability For Monitoring Drought-Related Vegetation Conditions, Trenton D. Benedict, Jesslyn F. Brown, Stephen P. Boyte, Daniel M. Howard, Brian A. Fuchs, Brian D. Wardlow, Tsegaye Tadesse, Kirk A. Evenson

National Drought Mitigation Center: Faculty Publications

Vegetation has been effectively monitored using remote sensing time-series vegetation index (VI) data for several decades. Drought monitoring has been a common application with algorithms tuned to capturing anomalous temporal and spatial vegetation patterns. Drought stress models, such as the Vegetation Drought Response Index (VegDRI), often use VIs like the Normalized Difference Vegetation Index (NDVI). The EROS expedited Moderate Resolution Imaging Spectrora-diometer (eMODIS)-based, 7-day NDVI composites are integral to the VegDRI. As MODIS satellite platforms (Terra and Aqua) approach mission end, the Visible Infrared Imaging Radiometer Suite (VIIRS) presents an alternate NDVI source, with daily collection, similar band passes, and …

Cytokine And Haptoglobin Profiles From Shipping Through Sickness And Recovery In Metaphylaxis- Or Un-Treated Cattle, Carol G. Chitko-Mckown, Gary L. Bennett, Larry A. Kuehn, Keith D. Dedonder, Michael D. Apley, Gregory P. Harhay, Michael L. Clawson, Aspen M. Workman, Bradley J. White, Robert L. Larson, Sarah F. Capik, Brian V. Lubbers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Fifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns located in Missouri, Tennessee, and Kentucky) enrolled in a study comparing animals receiving metaphylaxis to saline-treated controls. Cattle were transported to a feedlot in KS and assigned to treatment group. Blood samples were collected at Day 0 (at sale barn), Day 1, Day 9, and Day 28 (at KS feedlot), and transported to the …

Holocene Records Of Nebraska Mammals, Hugh H. Genoways

Zea E-Books Collection

A survey of the archeological and paleontological literature allowed a compilation of Holocene records of mammals in Nebraska. This survey identified Holocene records from 338 sites in 62 of the 93 Nebraska counties. These counties were located throughout state, but there was a concentration of sites in southwestern Nebraska where there were 27 fossil sites in Frontier County and 22 in Harlan County. Fossils sites were underrepresented in the Sand Hills region. Records of fossil mammals covered the entire Holocene period from 13,000 years ago until AD 1850. A minimum of 57 species (with eight additional species potentially present) representing …

Highlighting Membrane Protein Structure And Function: A Celebration Of The Protein Data Bank, Fei Li, Pascal F. Egea, Alex J. Vecchio, Ignacio Asial, Meghna Gupta, Joana Paulino, Ruchika Bajaj, Miles Sasha Dickinson, Shelagh Fergunson-Miller, Brian C. Monk, Robert M. Stroud

Department of Biochemistry: Faculty Publications

Biological membranes define the boundaries of cells and compartmentalize the chemical and physical processes required for life. Many biological processes are carried out by proteins embedded in or associated with such membranes. Determination of membrane protein (MP) structures at atomic or near-atomic resolution plays a vital role in elucidating their structural and functional impact in biology. This endeavor has determined 1198 unique MP structures as of early 2021. The value of these structures is expanded greatly by deposition of their three-dimensional (3D) coordinates into the Protein Data Bank (PDB) after the first atomic MP structure was elucidated in 1985. Since …

The Impact Of China’S Environmental And Trade Policies On U.S. Plastic And Paper Waste Exports, Savant Nzayiramya, John C. Beghin

Cornhusker Economics

This article documents major environmental and trade policy changes in China and their impact on plastic and paper waste trade between the U.S. and China and other trade partners. The article explains how U.S. plastic waste exports have been nearly annihilated by these policies. Paper waste trade is more resilient and has survived the policy shifts in China, although quite diminished.

Maldi-Tof Ms Biomarker Detection Models To Distinguish Rtx Toxin Phenotypes Of Moraxella Bovoculi Strains Are Enhanced Using Calcium Chloride Supplemented Agar, Matthew M. Hille, Michael L. Clawson, Aaron M. Dickey, Justin H. Lowery, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is the bacterium most often cultured from ocular lesions of cattle with infectious bovine keratoconjunctivitis, also known as bovine pinkeye. Some strains of M. bovoculi contain operons encoding for a repeats-in-toxin (RTX) toxin, which is a known virulence factor of multiple veterinary pathogens. We explored the utility of MALDI-TOF MS and biomarker detection models to classify the presence or absence of an RTX phenotype in M. bovoculi. Ninety strains that had undergone whole genome sequencing were classified by the presence or absence of complete RTX operons and confirmed with a visual assessment of hemolysis on blood agar. Strains …

Predictors Of Separation Anxiety In Dogs, Mckenna Yohe

Honors Program: Senior Projects (Public)

Understanding the potential causes and risk factors associated with separation anxiety in dogs is necessary to provide insight for possible prevention measures and to maintain the health and well-being of the animals affected by this condition. While a considerable amount of research has been done on this subject, it is still unclear what specific characteristics of dogs and their owners relate to the occurrence of separation anxiety and related behaviors. This study aimed to reexamine several dog and owner characteristics that have previously been evaluated in relation to the incidence of separation anxiety, as well as investigate how the frequency …

Free To Hate: Hate Crimes' Intertwinement With The Evolution Of Free Speech In The United States, Lee F. Paulson

Honors Program: Senior Projects (Public)

In response to the growing tension between civil liberties and civil rights, this research investigates the relationship between the relative expansiveness of free speech and a the nationwide propensity for hate crimes. I argue that government’s legal limitations of speech influence the development of linguistic and hierarchical norms in a national culture. Given structural inequality’s association to violence and crimes of intimidation, I hypothesize that as the government expands the legal bounds of free speech, the national propensity for hate crimes decreases. Text analyses of 50 influential freedom of expression rulings in the United States (U.S.) Supreme Court from 1919-2019 …

Learn, Try, Repeat: Experiential Learning In Adult Second Language Acquisition Of Spanish In Higher Education, Veronica Miller

Honors Program: Senior Projects (Public)

The purpose of this thesis is to synthesize research of experiential learning for adult Spanish learners in higher education to identify important takeaways and propose draft curriculum to improve acquisition for learners through experience-based and hands-on practices. Hopefully, this will aid in understanding, identify gaps in existing research, and better inform lesson-planning for instructors. My research does not include any comparison to other languages or other levels of education. I approach the issue through exploratory and descriptive research through open-source data retrieval by information obtained from governmental and nongovernmental resources.

Using Crispr-Cas9 To Construct Knockout Mutants In Dna-Repair Genes In Arabidopsis Thaliana, David Campbell

Honors Program: Senior Projects (Public)

The mitochondria are known as the powerhouse of the cell, and just like a real powerhouse, it can be a dangerous place to store sensitive information. Energy generation and redox reactions in the mitochondria can cause damage to the DNA stored there, resulting in a higher mutation rate. Compared to their animal counterparts, however, plant mitochondria exhibit a lower mutation rate and a higher recombination rate. It is hypothesized that the unique DNA repair methods present in plant mitochondria are responsible for the phenomena observed there. To study the mechanics of DNA-repair in this organelle, however, researchers must be able …

Distribution And Trends Of Endemic Hawaiian Waterbirds, Eben H. Paxton, Kevin Brinck, Adonia Henry, Afsheen Siddiqi, Rachel Rounds, Jennifer Chutz

United States Geological Survey: Staff Publications

Four endemic species of wetland-dependent waterbirds occur on the main Hawaiian Islands, all of which have experienced sharp population declines and are listed as endangered species. Twice per year, state-wide surveys are conducted to count waterbirds, but these surveys are evaluated only infrequently. We used a state-space approach to evaluate long-term (1986–2016) and short-term (2006–2016) trends and current distribution and abundance of endemic Hawaiian waterbirds. The most numerous species was the Ae‘o, or Hawaiian Stilt (Himantopus mexicanus knudseni), with a 5-year estimated average abundance of 1,932 individuals, followed by ‘Alae Ke‘oke‘o, or Hawaiian Coot (Fulica alai), …

The Interplay Of Emotion, Cognition, And Learning In The Language Classroom, Aleidine J. Moeller

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Challenge Statement Emotions are inextricably linked to our actions, behaviors, and dispositions. To promote deeper learning, emotion and cognition must be in sync to maximize learning. How can we connect our learners’ emotions in ways that fully capitalize on the interplay with cognition and engages them in the language learning process?

Abstract This article seeks to broaden the discourse on world language teaching to take a more holistic view of learning and teaching that supports and promotes the integration of feeling and thinking. A summary of the research on the role of emotions in learning is documented and classroom examples …

Rehabilitation For Anterior Cruciate Ligament Tears: A Written And Video Guide For Athletes Today And In The Future, Braeland Jones

Honors Program: Senior Projects (Public)

The following paper is a partner to a corresponding video exercise guide. The purpose of this paper is to support the exercises included in the guide with research. The research paper aids in defining which exercises are included in the first place as well as in which section they appear. The research is the literary match for the recorded exercise guide, which will be the visual side of the project. The two ideally should be read and watched together to fully understand content of both portions. The paper guide is split into three sections, evaluating the types of exercises and …

Defining And Validating Regenerative Farm Systems Using A Composite Of Ranked Agricultural Practices, Tommy L.D. Fenster, Claire E. Lacanne, Jacob R. Pecenka, Ryan B. Schmid, Michael M. Bredeson, Katya M. Busenitz, Alex M. Michels, Kelton D. Welch, Johnathan G. Lundgren

University of Nebraska State Museum: Entomology Papers

Background: Ongoing efforts attempt to define farms as regenerative to aid marketers, policymakers, farmers, etc. The approach needs to balance precision with function, and must be transparent, simple, scalable, transferable, incorruptible, and replicable. Methods: We developed practice-based scoring systems to distinguish regenerative cropland and rangeland, and validate them based on whether these scores scaled with regenerative goals on actual farm operations. Study systems included cornfields of the Upper Midwest, almond orchards of California, and rangeland systems of the Northern Plains. Response variables included soil carbon and organic matter, soil micronutrients, water infiltration rates, soil microbial communities, plant community structure, invertebrate …

Effects Of Intraguild Interactions On Anticarsia Gemmatalis And Chrysodeixis Includens Larval Fitness And Behavior In Soybean, Sabrina Ongaratto, Edson Ll Baldin, Thomas E. Hunt, Debora G. Montezano, Emily A. Robinson, Maria C. Dos Santos

Department of Agronomy and Horticulture: Faculty Publications

BACKGROUND: Crop pest management requires an understanding of the complex interactions among species that potentially impact crop yield. In soybean, the velvetbean caterpillar, Anticarsia gemmatalis (Hübner), and the soybean looper, Chrysodeixis includens (Walker), are described as key pests, sharing the same feeding guild. We assessed the intraguild interactions of these species under laboratory conditions. Fitness cost study was conducted to examine the influence of competition on insect development. A video tracking system was used to evaluate behavioral parameters during larval interactions in scenarios with and without food availability. RESULTS: In the fitness cost assay, pupal weight was not significantly affected, …

The Impact Of Covid-19 And Economic Policies On Nebraska Farm Real Estate In 2021, Jim Jansen, Jeffrey Stokes

Cornhusker Economics

Land industry professionals responding to the annual survey attributed the rise in Nebraska farm real estate values to current interest rate levels, crop prices, and COVID-19 disaster assistance payments provided to operators across the state. These forces substantially impacted farm and ranch finances across Nebraska. Operators faced an exceptional amount of risk and uncertainty when navigating production decisions during the height of the COVID-19 pandemic. Lower interest rates and disaster assistance payments provided stability to an industry facing an unpreceded economic shutdown and disruptions of supply chains.

Term Limits, Political Polarization, And Voter Behavior: An Analysis Of The Nebraska Unicameral, Jared Long

Honors Program: Senior Projects (Public)

Term limits are an often-debated reform proposal in American politics. In the 1990s and 2000s, many states adopted a range of term limit policies, including Nebraska. At the time, many bold predictions were made for how such a significant structural change in state governance might affect political norms. Over the past 20 to 30 years, many empirical studies have been carried out to weigh the merits of these predictions. However, much research has focused on institutional effects within state legislatures themselves; less focus has been given to the residual effects on voters themselves.

This paper posits the argument that term …

De Novo Assembly And Annotation Of The North American Bison (Bison Bison) Reference Genome And Subsequent Variant Identification, L K. Dobson, A Zimin, D Bayles, E Fritz-Waters, D Alt, S Olsen, J Blanchong, J Reecy, T Pl Smith, J N. Derr

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Genomic tools have improved the ability to manage bison populations and enhanced efforts to conserve this iconic species. These tools have been particularly useful for detecting introgression of cattle genome within bison herds but are limited by the need to use the cattle genome as a surrogate for mapping reads. This complicates efforts to distinguish the species of origin of chromosomal segments in individual bison at the genomic level. An assembly (Bison_UMD1.0) based on 75X genome coverage by Illumina and 454 reads was generated using the MaSuRCA assembler, generating a 2.81 Gigbases de novo reference genome from American bison. Comparison …

Variations In Bacterial Community Structure And Antimicrobial Resistance Gene Abundance In Cattle Manure And Poultry Litter, Biyensa Gurmessa, Amanda J. Ashworth, Yichao Yang, Mary Savin, Philip A. Moore Jr, Steven C. Ricke, Giuseppe Corti, Ester Foppa Pedretti, Stefania Cocco

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cattle manure and poultry litter are widely used as fertilizers as they are excellent sources of nutrients; however, potential adverse environmental effects exist during land applications, due to the release of zoonotic bacteria and antimicrobial resistance (AMR) genes. This study was conducted to understand linkages between physiochemical composition, bacterial diversity, and AMR gene presence of cattle manure and poultry litter using quantitative polymerase chain reaction to enumerate four AMR genes (ermB, sulI, intlI, and blactx-m-32), Illumina sequencing of the 16 S region, and analysis of physical and chemical properties. Principal coordinate analysis of Bray–Curtis distance revealed distinct bacterial community structures …

Microbial Community Field Surveys Reveal Abundant Pseudomonas Population In Sorghum Rhizosphere Composed Of Many Closely Related Phylotypes, Dawn Chiniquy, Elle M. Barnes, Jinglie Zhou, Kyle Hartman, Xiaohui Li, Amy Sheflin, Ellen Marsh, Jessica Prenni, Adam M. Deutschbauer, Daniel P. Schachtman, Susannah G. Tringe

Department of Agronomy and Horticulture: Faculty Publications

While the root-associated microbiome is typically less diverse than the surrounding soil due to both plant selection and microbial competition for plant derived resources, it typically retains considerable complexity, harboring many hundreds of distinct bacterial species. Here, we report a time-dependent deviation from this trend in the rhizospheres of field grown sorghum. In this study, 16S rRNA amplicon sequencing was used to determine the impact of nitrogen fertilization on the development of the root-associated microbiomes of 10 sorghum genotypes grown in eastern Nebraska. We observed that early rhizosphere samples exhibit a significant reduction in overall diversity due to a high …