Digital Commons Network^™

Testing Oxygenated Microbubbles Via Intraperitoneal And Intrathoracic Routes On A Large Pig Model Of Lps-Induced Acute Respiratory Distress Syndrome, Riaz Ur Rehman Mohammed, Nathaniel T. Zollinger, Andrea R. Mccain, Roser Romaguera-Matas, Seth P. Harris, Keely L. Buesing, Mark A. Borden, Benjamin S. Terry

Department of Mechanical and Materials Engineering: Faculty Publications

With a mortality rate of 46% before the onset of COVID-19, acute respiratory distress syndrome (ARDS) affected 200,000 people in the US, causing 75,000 deaths. Mortality rates in COVID-19 ARDS patients are currently at 39%. Extrapulmonary support for ARDS aims to supplement mechanical ventilation by providing life-sustaining oxygen to the patient. A new rapid-onset, human-sized pig ARDS model in a porcine intensive care unit (ICU) was developed. The pigs were nebulized intratracheally with a high dose (4 mg/kg) of the endotoxin lipopolysaccharide (LPS) over a 2 h duration to induce rapid-onset moderate-to- severe ARDS. They were then catheterized to monitor …

Circadian Rhythms Mediate Infection Risk In Daphnia Dentifera, Alaina C. Pfenning-Butterworth, David T. Nguyen, Jessica L. Hite, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Biological rhythms mediate important within-host processes such as metabolism, immunity, and behavior which are often linked to combating disease exposure. For many hosts, exposure to pathogens occurs while feeding. However, the link between feeding rhythms and infection risk is unclear because feeding behavior is tightly coupled with immune and metabolic processes which may decrease susceptibility to infection. Here, we use the Daphnia dentifera–Metschnikowia bicuspidata host–pathogen system to determine how rhythms in feeding rate and immune function mediate infection risk. The host is known to have a nocturnal circadian rhythm in feeding rate, yet we found that they do not exhibit …

Type 2 Polysaccharide Storage Myopathy In Quarter Horses Is A Novel Glycogen Storage Disease Causing Exertional Rhabdomyolysis, Stephanie J. Valberg, Zoë J. Williams, Carrie J. Finno, Abigail Schultz, Deborah Velez-Irizarry, Marisa L. Henry, Keri Gardner, Jessica Lynn Petersen

Department of Animal Science: Faculty Publications

Background: Both type 1 (PSSM1) and type 2 polysaccharide storage myopathy (PSSM2) are characterised by aggregates of abnormal polysaccharide in skeletal muscle. Whereas the genetic basis for PSSM1 is known (R309H GYS1), the cause of PSSM2 in Quarter Horses (PSSM2-QH) is unknown and glycogen concentrations not defined.

Objectives: To characterise the histopathological and biochemical features of PSSM2-QH and determine if an associated monogenic variant exists in genes known to cause glycogenosis.

Study design: Retrospective case control.

Methods: Sixty-four PSSM2-QH, 30 PSSM1-QH and 185 control-QH were identified from a biopsy repository and clinical data, histopathology scores …

Bacterial Diseases Of Tilapia, Their Zoonotic Potential And Risk Of Antimicrobial Resistance, Olga L. M. Haenen, Ha Thanh Dong, Truong Dinh Hoai, Margaret Crumlish, Iddya Karunasagar, Timothy Barkham, Swaine L. Chen, Ruth Zadoks, Andreas Kiermeier, Bing Wang, Esther Garrido Gamarro, Masami Takeuchi, Mohammad Noor Amal Azma, Belén Fouz, Southeast Asian Fisheries Development Center, Zeng Wei Wei, Melba G. Bondad-Reantaso

School of Natural Resources: Faculty Publications

Tilapia culture is an important source of income and nutrition to many rural families. Since 2000, the production of tilapia increased and reached domestic and global markets. Major farmed species is Nile tilapia (Oreochromis niloticus), in earthen ponds and cage cultures. Intensification contributed to global tilapia disease outbreaks, with bacterial infections causing mortalities and morbidities, threatening sustainable production. At tilapia farms, high nutrient concentrations, water temperature and fish densities enhance bacterial growth including virulent bacterial clones and potential zoonotic bacteria. Global warming favours this. This review respectively provides a comprehensive overview of the most common and emerging bacterial …

Dataset For Large-Scale, Lateral-Torsional Buckling Tests Of Continuous Beams In A Grillage System, Ahmed Rageh, C. Shawn Sun, Daniel G. Linzell, Jay A. Puckett

Department of Civil and Environmental Engineering: Faculty Publications

A number of large-scale tests of a grillage system are summarized and reduced data are provided. The tests were completed in association with a Louisiana Transportation Research Center (LTRC) project for the Louisiana Department of Transportation and Development (LA DOTD) whose goal was to better quantify behavior and limit states of steel bridge floor systems to refine longitudinal member (stringer) load- rating calculations. A number of tests focused on the behavior of the stringers resting on transverse members (a floorbeam), a system akin to a grillage. The system was proportioned with the expectation that stringer lateral-torsional buckling, a key steel …

Protecting Our Pets: Nebraska’S Domestic Abuse Protection Orders And Pets, Josiah J. Shanks

Nebraska Law Bulletin (Selected Issues)

It is now well-understood that there is a link between violence toward animals and community-involved violence. And abusers typically escalate their abuse as it goes on. Unfortunately, abuse victims frequently stay within the confines of their relationship out of fear, especially when pets are involved. Protection orders are effective at protecting abuse victims, and their pets, from abusers.

Pets play a unique role in our lives and the law. Pets need to be expressly accounted for within protection order statutes. Half-measures, such as relying on a catch-all provision or updating protection order forms, are not enough. Currently, Nebraska’s protection order …

Circadian Rhythms Mediate Infection Risk In Daphnia Dentifera, Alaina C. Pfenning-Butterworth, David T. Nguyen, Jessica L. Hite, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Biological rhythms mediate important within-host processes such as metabolism, immunity, and behavior which are often linked to combating disease exposure. For many hosts, exposure to pathogens occurs while feeding. However, the link between feeding rhythms and infection risk is unclear because feeding behavior is tightly coupled with immune and metabolic processes which may decrease susceptibility to infection. Here, we use the Daphnia dentifera–Metschnikowia bicuspidata host–pathogen system to determine how rhythms in feeding rate and immune function mediate infection risk. The host is known to have a nocturnal circadian rhythm in feeding rate, yet we found that they do not exhibit …

Circadian Rhythms Mediate Infection Risk In Daphnia Dentifera, Alaina C. Pfenning-Butterworth, David T. Nguyen, Jessica L. Hite, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Biological rhythms mediate important within-host processes such as metabolism, immunity, and behavior which are often linked to combating disease exposure. For many hosts, exposure to pathogens occurs while feeding. However, the link between feeding rhythms and infection risk is unclear because feeding behavior is tightly coupled with immune and metabolic processes which may decrease susceptibility to infection. Here, we use the Daphnia dentifera–Metschnikowia bicuspidata host–pathogen system to determine how rhythms in feeding rate and immune function mediate infection risk. The host is known to have a nocturnal circadian rhythm in feeding rate, yet we found that they do not exhibit …

Time Domains Of Hypoxia Responses And -Omics Insights, James J. Yu, Amy L. Non, Erica C. Heinrich, Wanjun Gu, Joe Alcock, Esteban A. Moya, Elijah S. Lawrence, Michael S. Tift, Katie A. O'Brien, Jay F. Storz, Anthony V. Signore, Jane I. Khudyakov, William K. Milsom, Sean M. Wilson, Cynthia M. Beall, Francisco C. Villafuerte, Tsering Stobdan, Colleen G. Julian, Lorna G. Moore, Mark M. Fuster, Jennifer A. Stokes, Richard Milner, John B. West, Jiao Zhang, John Y. Shyy, Ainash Childebayeva, José Pablo Vázquez-Medina, Luu V. Pham, Omar A. Mesarwi, James E. Hall, Zachary A. Cheviron, Jeremy Sieker, Arlin B. Blood, Jason X. Yuan, Tatum S. Simonson, Et Al.

School of Biological Sciences: Faculty Publications

The ability to respond rapidly to changes in oxygen tension is critical for many forms of life. Challenges to oxygen homeostasis, specifically in the contexts of evolutionary biology and biomedicine, provide important insights into mechanisms of hypoxia adaptation and tolerance. Here we synthesize findings across varying time domains of hypoxia in terms of oxygen delivery, ranging from early animal to modern human evolution and examine the potential impacts of environmental and clinical challenges through emerging multi-omics approaches. We discuss how diverse animal species have adapted to hypoxic environments, how humans vary in their responses to hypoxia (i.e., in the context …

Low Levels Of Hybridization Between Sympatric Cold-Water-Adapted Arctic Cod And Polar Cod In The Beaufort Sea Confirm Genetic Distinctiveness, Robert E. Wilson, Sarah A. Sonsthagen, Philip Lavretsky, Andrew Majewski, Einar Árnason, Katrín Halldórsdóttir, Axel W. Einarsson, Kate Wedemeyer, Sandra L. Talbot

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

As marine ecosystems respond to climate change and other stressors, it is necessary to evaluate current and past hybridization events to gain insight on the outcomes and drivers of such events. Ancestral introgression within the gadids has been suggested to allow cod to inhabit a variety of habitats. Little attention has been given to contemporary hybridization, especially within cold-water-adapted cod (Boreogadus saida Lepechin, 1774 and Arctogadus glacialis Peters, 1872). We used whole-genome, restriction-site associated, and mitochondrial sequence data to explore the degree and direction of hybridization between these species where previous hybridization had not been reported. Although nearly identical …

A Checklist Of Parasites Of Peromyscus Maniculatus In North America, John Ubelaker, Gábor R. Rácz

MANTER: Journal of Parasite Biodiversity

This document represents a summary of parasites, in the broadest sense of the term, reported from Peromyscus maniculatus from throughout its range in North America. The document provides data from work ranging from paleontological findings to relatively current reports of parasites and parasitism from P. maniculatus and covers viruses, bacteria sensu lato, protists, helminths, and ectoparasites.

18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska

Department of Plant Pathology: Faculty Publications

Nematodes are the most abundant and diverse animals on the planet but lack representation in biodiversity research. This presents a problem for studying nematode diversity, particularly when molecular tools (i.e., barcoding and metabarcoding) rely on well-populated and curated reference databases, which are absent for nematodes. To improve molecular identification and the assessment of nematode diversity, we created and curated an 18S rRNA database specific to nematodes (18S-NemaBase) using sequences sourced from the most recent publicly available 18S rRNA SILVA v138 database. As part of the curation process, taxonomic strings were standardized to contain a fixed number of taxonomic ranks relevant …

Nebline, August/September 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Nutrition Security: A New National Focus by Emily Gratopp

Recipe of the Month: Starwberry Smoothie and Freezer Pop by Emily Gratopp

Harvesting & Drying Herbs by Sarah Browning

Garden Guide: Things to Do This Month

Water Play Fun by Hayley Jackson

Controlling Poison Hemlock by Melissa Bartels

Summer Brings Singing Cicadas by Kait Chapman

4-H Announcements for Enrolled 4-H'ers or 4-H Volunteers

Lancaster County Super Fair, July 28-Aug. 6, 2022

4-H Will Livestream from the Fair! YouTube

Join 4-H!

Heart of 4-H Volunteer Award: Sonja Moore and Lancaster County Engineering Department

4-H Teen Council Invites New Members

4-H Council Golf …

Fire And Human Management Of Late Holocene Ecosystems In Southern Africa, Benjamin Davies, Mitchell J. Power, David R. Braun, Matthew J. Douglass, Stella G. Mosher, Lynne J. Quick, Irene Esteban, Judith Sealy, John Parkington, J. Tyler Faith

School of Natural Resources: Faculty Publications

Globally, fire is a primary agent for modifying environments through the long-term coupling of human and natural systems. In southern Africa, control of fire by humans has been documented since the late Middle Pleistocene, though it is unclear when or if anthropogenic burning led to fundamental shifts in the region's fire regimes. To identify potential periods of broad-scale anthropogenic burning, we analyze aggregated Holocene charcoal sequences across southern Africa, which we compare to paleoclimate records and archaeological data. We show climate-concordant variability in mid-Holocene fire across much of the subcontinent. However, increased regional fire activity during the late Holocene (~2000 …

Women Of The Wolf, Rosemary Sekora

Department of English: Dissertations, Theses, and Student Research

This is a creative thesis that introduces the novel Women of the Wolf and discusses the writer’s influences and research progress. Themes within the novel will include women relationships, cult culture, religious influences, and Native American (mis)representation. The sample included is the first ten pages from the novel.

Advisor: Timothy Schaffert

Vitamin And Mineral Supplementation And Rate Of Gain In Beef Heifers Ii: Effects On Concentration Of Trace Minerals In Maternal Liver And Fetal Liver, Muscle, Allantoic, And Amniotic Fluids At Day 83 Of Gestation, Kacie L. Mccarthy, Ana Clara Ana, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, Alison K. Ward, Pawel P. Borowicz, Lawrence P. Reynolds, Kevin K. Sedivec, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen, Kacie L. Mccarthy

Department of Animal Science: Faculty Publications

We evaluated the effects of vitamin and mineral supplementation (from pre-breeding to day 83 of gestation) and two rates of gain (from breeding to day 83 of gestation) on trace mineral concentrations in maternal and fetal liver, fetal muscle, and allantoic (ALF) and amniotic (AMF) fluids. Crossbred Angus heifers (n = 35; BW = 359.5 ± 7.1 kg) were randomly assigned to one of two vitamin and mineral supplementation treatments (VMSUP; supplemented (VTM) vs. unsupplemented (NoVTM)). The VMSUP factor was initiated 71 to 148 d before artificial insemination (AI), allowing time for the mineral status of heifers to be altered …

Yearling Bulls Have Reduced Sperm Concentration And Increased Seminal Plasma Interleukin-8 After A 28-Day Breeding Season, Alexandria P. Snider, Kacie L. Mccathy, Elane C. Wright-Johnson, Jeffrey D. Ondrak, William T. Oliver, Carl R. Dahlen, Robert A. Cushman, Matthew S. Crouse

Department of Animal Science: Faculty Publications

We hypothesized that yearling bulls selected for a 28-d breeding season would have reduced sperm concentrations and morphology, and have increased seminal plasma concentrations of pro-inflammatory cytokine interleukin-8 (IL-8). Yearling bulls were selected based on a breeding soundness examination (BSE) at approximately 415 d of age and contained at least 750 million sperm in the ejaculate, with 12 bulls randomly selected for breeding (BREEDERS) and 12 bulls not selected for breeding (NON-BREEDERS). After a 28-d breeding period, all bulls underwent a BSE. Plasma and seminal plasma were collected at each time point for analysis. Data were analysed utilizing either the …

Primary Myoblasts From Intrauterine Growth-Restricted Fetal Sheep Exhibit Intrinsic Dysfunction Of Proliferation And Differentiation That Coincides With Enrichment Of Inflammatory Cytokine Signaling Pathways, Robert J. Posont, Micah S. Most, Caitlin Cadaret, Eileen Marks-Nelson, Kiristen A. Beede, Sean W. Limesand, Ty B. Schmidt, Jessica L. Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Intrauterine growth restriction (IUGR) is linked to lifelong reductions in muscle mass due to intrinsic functional deficits in myoblasts, but the mechanisms underlying these deficits are not known. Our objective was to determine if the deficits were associated with changes in inflammatory and adrenergic regulation of IUGR myoblasts, as was previously observed in IUGR muscle. Primary myoblasts were isolated from IUGR fetal sheep produced by hyperthermia-induced placental insufficiency (PI-IUGR; n = 9) and their controls (n = 9) and from IUGR fetal sheep produced by maternofetal inflammation (MI-IUGR; n = 6) and their …

Vitamin And Mineral Supplementation And Rate Of Weight Gain During The First Trimester Of Gestation In Beef Heifers Alters The Fetal Liver Amino Acid, Carbohydrate, And Energy Profile At Day 83 Of Gestation, Matthew S. Crouse, Kacie L. Mccarthy, Ana Clara B. Menezes, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri Dorsam, Tammi L. Neville, Alison K. Ward, Pawel P. Borowicz, Lawrence P. Reynolds, Kevin K. Sedivec, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

The objective of this study was to evaluate the effects of feeding heifers a vitamin and mineral supplement and targeting divergent rates of weight gain during early gestation on the fetal liver amino acid, carbohydrate, and energy profile at d 83 of gestation. Seventy-two crossbred Angus heifers were randomly assigned in a 2 × 2 factorial arrangement to one of four treatments comprising the main effects of vitamin and mineral supplementation (VTM or NOVTM) and feeding to achieve different rates of weight gain (low gain [LG] 0.28 kg/day vs. moderate gain [MG] 0.79 kg/day). Thirty-five gestating heifers with female fetuses …

A Survey Of The Microbial Communities Of Commercial Presliced, Packaged Deli-Style Ham Throughout Storage, Rebecca A. Furbeck, Chad G. Bower, Samodha C. Fernando, Gary Sullivan

Department of Animal Science: Faculty Publications

The goal of this study was to evaluate the variation in spoilage microbiota associated with sliced, prepackaged deli-style ham from varying processing environments available in the retail market in the United States. Three different brands of presliced ham, water added were purchased at local markets and evaluated every 2 wk beginning 4 wk prior to the sell-by date until 4 wk beyond the sell-by date. Analysis of 16S ribosomal RNA genes using operational taxonomic units showed that Brand A had a different bacterial community structure compared with Brands B and C, according to unweighted (P = 0.006) and weighted …

Characterization And Selection Of Hop Cultivars Adapted To Nebraska, Kristina Alas

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Hop (Humulus lupulus L.) is an ingredient in the beer brewing industry that provides beer its flavor and aroma. High demand from the brewing industry has encouraged production outside of the traditional Pacific Northwest, the primary production region. Producers in the Midwest are attempting to grow cultivars adapted to the Pacific Northwest, but environmental differences have caused low yields and changes in secondary metabolite content. To aid producers, a regional breeding program was initiated to develop cultivars adapted to the Midwest. Success of any breeding program relies on the selection of genetically superior parents to generate progeny with the …

Comparative Assessment Of Human Exposure To Antibiotic-Resistant Salmonella Due To The Consumption Of Various Food Products In The United States, Yifan Wu

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Antibiotic resistance (AR) was increasingly recognized as a global and national problem. Prevention efforts are hampered by a lack of complete understanding of how transmission pathways contribute to human AR exposure. Many reports have indicated the presence of antibiotic-resistant bacteria in foods at retail, suggesting that food consumption, animal-derived foods in particular, can represent a significant source of AR exposure among consumers. The presence of Salmonella, including antibiotic-resistant Salmonella, has been frequently reported in terrestrial animal-derived foods such as meat, poultry, and dairy products, as well as in aquaculture products. Identification of the significant food sources that harbor …

Experiences With Vitamin A In Copperbelt Province, Zambia, Topacio Ortiz

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Vitamin A deficiency (VAD) is one of the most common micronutrient deficiencies in Sub-Saharan Africa. Zambians face high VAD rates, despite a mandate for fortified sugar and crops. Limited data have been collected from the most vulnerable segments of Zambia’s population; hence this study was designed as an initiatory study for future research to be conducted with primary school children and pregnant women in Zambian communities.

Once IRB approvals were obtained from the University of Nebraska-Lincoln (UNL) and the Copperbelt University School of Medicine, data were collected in Zambia’s Copperbelt Province, in the cities of Ndola and Luanshya, during May …

Nonbreeding Season Survival And Habitat Selection Of Northern Bobwhite In Northeastern Colorado, Joseph M. Wolske

School of Natural Resources: Dissertations, Theses, and Student Research

Northern bobwhites (Colinus virginianus) have experienced range-wide population declines. Recent harvest data from northeastern Colorado suggests bobwhite populations have declined and populations can be sensitive to adult nonbreeding season survival. We monitored 157 bobwhites in northeastern Colorado, on the northern periphery of the species’ range, for the 2019-2020 and 2020-2021 nonbreeding seasons to estimate nonbreeding season survival and habitat selection. We used known-fate survival models to assess any variation in survival between the winter stages of early-winter, mid-winter, and late-winter, as well as sex, age class, and mass at the time of capture. Survival varied among winter stages, …

Human Perception Of Exponentially Increasing Data Displayed On A Log Scale Evaluated Through Experimental Graphics Tasks, Emily Robinson

Department of Statistics: Dissertations, Theses, and Student Research

Log scales are often used to display data over several orders of magnitude within one graph. We conducted a series of three graphical studies to evaluate the impact displaying data on the log scale has on human perception of exponentially increasing trends compared to displaying data on the linear scale. Each study was related to a different graphical task, each requiring a different level of interaction and cognitive use of the data being presented. The first experiment evaluated whether our ability to perceptually notice differences in exponentially increasing trends is impacted by the choice of scale. Participants were shown a …

Analyzing Flexibility As A Risk Management Strategy In Agricultural Systems, Jedidiah Hewlett

Department of Agricultural Economics: Dissertations, Theses, and Student Research

Agricultural businesses deal with uncertainty of all kinds. Uncertainty arising from variability in resources, markets, human factors, and regulatory policy can be sources of significant risks. Acquiring or building flexibility in an operation is one of the ways suggested to manage risk and uncertainty—reducing the impact of negative outcomes and increasing the benefit of positive outcomes. Although flexibility has been repeatedly encouraged for businesses, the definition of flexibility and its value remains ambiguous after more than 80 years of research. Flexibility is often described as a multi-dimensional ability to effectively deal with change in the business operating environment. Flexibility can …

Production Of Polyhydroxybutyrate From Corn Fiber Using Paraburkholderia Sacchari, Twissa Mitra

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Poly(3-hydroxybutyrate) (PHB) is one of the most broadly studied members of the polyhydroxyalkanoates (PHA) family. It is a fully biodegradable thermoplastic produced by microbial fermentation having properties similar to polypropylene. PHB has a market price that is three times higher than the cost of synthetic plastics. Substrate cost makes up 50 to 60 % of that cost; hence, researchers have been exploring various cheap natural resources with high carbohydrate content to be used as a substrate for PHB production.

In this study, corn fiber, a lignocellulosic biomass has been studied as an option for a substrate to produce PHB. This …

18s-Nemabase: Curated 18s Rrna Database Of Nematode Sequences, Kaitlin Gattoni, Eli M. S. Gendron, Rebeca Sandoval- Ruiz, Abigail Borgemeier, J. Parr Mcqueen, Rachel M. Shepherd, Dieter Slos, Tom Powers, Dorota L. Porazinska

Department of Plant Pathology: Faculty Publications

Nematodes are the most abundant and diverse animals on the planet but lack representation in biodiversity research. This presents a problem for studying nematode diversity, particularly when molecular tools (i.e., barcoding and metabarcoding) rely on well-populated and curated reference databases, which are absent for nematodes. To improve molecular identification and the assessment of nematode diversity, we created and curated an 18S rRNA database specific to nematodes (18S-NemaBase) using sequences sourced from the most recent publicly available 18S rRNA SILVA v138 database. As part of the curation process, taxonomic strings were standardized to contain a fixed number of taxonomic ranks relevant …

In Vivo Efficacy Of Pyrantel Pamoate As A Post-Exposure Prophylactic For Rat Lungworm (Angiostrongylus Cantonensis), John Jacob, Argon Steel, Lisa Kaluna, Steven Hess, Israel Leinbach, Carmen Antaky, Robert Sugihara, Lindsey Hamilton, Peter Follett, Kathleen Howe, Steven Jacquier, Supakit Wongwiwatthananukit, Susan Jarvi

United States Department of Agriculture Wildlife Services: Staff Publications

Rat lungworm (Angiostrongylus cantonensis) is a neurotropic nematode, and the leading cause of eosinophilic meningitis worldwide. The parasite is usually contracted through ingestion of infected gastropods, often hidden in raw or partially cooked produce. Pharmaceutical grade pyrantel pamoate was evaluated as a post-exposure prophylactic against A. cantonensis. Pyrantel pamoate is readily available over-the-counter in most pharmacies in the USA and possesses anthelmintic activity exclusive to the gastrointestinal tract (GIT). Administering pyrantel pamoate immediately after exposure should theoretically paralyze the larvae in the GIT, causing the larvae to be expelled via peristalsis without entering the systemic circulation. In this study, pyrantel …

Current Ecotoxicity Testing Needs Among Selected U.S. Federal Agencies, Patricia Ceger, Natalia Garcia-Reyero Vinas, David Allen, Elyssa Arnold, Raanan Bloom, Jennifer C. Brennan, Carol Clarke, Karen Eisenreich, Kellie Fay, Jonathan Hamm, Paula F.P. Henry, Katherine E. Horak, Wesley Hunter, Donna Judkins, Patrice Klein, Nicole Kleinstreuer, Kara Koehrn, Carlie A. Lalone, James P. Laurenson, Jessica K. Leet, Anna Lowit, Scott G. Lynn, Teresa Norberg-King, Edward J. Perkins, Elijah J. Petersen, Barnett A. Rattner, Catherine S. Sprankle, Thomas Steeger, Jim E. Warren, Sarah Winfield, Edward Odenkirchen

United States Department of Agriculture Wildlife Services: Staff Publications

U.S. regulatory and research agencies use ecotoxicity test data to assess the hazards associated with substances that may be released into the environment, including but not limited to industrial chemicals, pharmaceuticals, pesticides, food additives, and color additives. These data are used to conduct hazard assessments and evaluate potential risks to aquatic life (e.g., invertebrates, fish), birds, wildlife species, or the environment. To identify opportunities for regulatory uses of non-animal replacements for ecotoxicity tests, the needs and uses for data from tests utilizing animals must first be clarified. Accordingly, the objective of this review was to identify the ecotoxicity test data …