Digital Commons Network^™

Herbicide Drift Vs. Crop Resilience – The Influence Of Micro-Rates, Milan Brankov, Bruno Canella Vieira, Miloš Rajković, Milena Simić, Vukadinović Jelena, Violeta Mandić, Vesna Dragičević

Department of Agronomy and Horticulture: Faculty Publications

A greenhouse study was conducted to test the effects of low herbicide dose exposure on different crops measuring visible damages, plant height, leaf area, and dry matter. Seven crops were tested: lettuce (Lactuca sativa L.) cv. Novosadska majska maslena, oil pumpkin (Cucurbita maxima Duch) cv. Olivija, oilseed rape (Brassica napus L.) cv. NS Ras, pepper (Capsicum annuum L.) cv. Kurtovska kapija, soybean (Glycine max (L.) Merr) cv. ZP Laura, sunflower (Helianthus annuus L.) cv. NS Kruna, and tomato (Solanum lycopersicum L.) cv. Dunavski Rubin. Herbicide dicamba in the range of 0.14 to 1 …

Fusarium Species Structure In Nebraska Corn, Yuchu Ma

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Fusarium species are known to infect corn and cause significant yield losses and mycotoxin contamination worldwide. In this study, we investigated the diversity of Fusarium species infecting corn in Nebraska and their potential to produce fumonisins and trichothecenes. A total of 259 Fusarium isolates were collected from different corn tissues (ear, stalk, and root), revealing a significant association between the various Fusarium species complexes and different plant parts (p < 0.05). Fusarium incarnatum-equiseti species complex (FIESC) was the most widespread and abundant, followed by the Fusarium sambucinum (FSAMSC) and Fusarium fujikuroi species complexes (FFSC). In the subsequent analysis, we investigated the mycotoxin …

The Rna Cargo In Small Extracellular Vesicles From Chicken Eggs Is Bioactive In C57bl/6j Mice And Human Peripheral Blood Mononuclear Cells Ex Vivo, Deborah Fratantonio, Javaria Munir, Jiang Shu, Katherine Howard, Scott R. Baier, Juan Cui, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Small extracellular vesicles (sEVs) and their RNA cargo in milk are bioavailable in humans, pigs, and mice, and their dietary depletion and supplementation elicits phenotypes. Little is known about the content and biological activity of sEVs in foods of animal origin other than milk. Here we tested the hypothesis that sEVs in chicken eggs (Gallus gallus) facilitate the transfer of RNA cargo from an avian species to humans and mice, and their dietary depletion elicits phenotypes. sEVs were purified from raw egg yolk by ultracentrifugation and authenticated by transmission electron microscopy, nano-tracking device, and immunoblots. The miRNA profile …

Am I Another You?, Laura Diane Cobb

School of Art, Art History, and Design: Theses and Student Creative Work

On my birth certificate are the names of my parents, though the name of my father stands in opposition to my genealogy. The script of his signature conceals my birth as donor conceived (DC). The truth of my origin would lay dormant for years behind his scrawl, burying my true heritage beneath the stories of my social father’s ancestry.

Learning the truth, I began to reevaluate my identity. Searching for myself along waterways, I explored the shores of the Platte River as if by knowing its sandbars, flora, and fauna, I would come to know myself. In searching the land, …

A Ferritin Nanoparticle-Based Zika Virus Vaccine Candidate Induces Robust Humoral And Cellular Immune Responses And Protects Mice From Lethal Virus Challenge, Aryamav Pattnaik, Bikash R. Sahoo, Lucas R. Struble, Gloria E. O. Borgstahl, You Zhou, Rodrigo Franco, Raul G. Barletta, Fernando A. Osorio, Thomas M. Petro, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The severe consequences of the Zika virus (ZIKV) infections resulting in congenital Zika syndrome in infants and the autoimmune Guillain–Barre syndrome in adults warrant the development of safe and efficacious vaccines and therapeutics. Currently, there are no approved treatment options for ZIKV infection. Herein, we describe the development of a bacterial ferritin-based nanoparticle vaccine candidate for ZIKV. The viral envelope (E) protein domain III (DIII) was fused in-frame at the amino-terminus of ferritin. The resulting nanoparticle displaying the DIII was examined for its ability to induce immune responses and protect vaccinated animals upon lethal virus challenge. Our results show that …

Biodiversity Benefits Of An Ecosystem Engineer Are Negated By An Invasive Predator, E. Claire Watersmith, Brandon Mcdonald, Jeremy Dixon, Michael V. Cove

United States Fish and Wildlife Service: Publications

Ecosystem engineers play a vital role in community assembly by modifying the environment to create novel habitat features. Woodrats (Neotoma sp.) build and maintain intricate stick-nests that stockpile organic materials and create habitat for other small species. The Key Largo woodrat (Neotoma floridana smalli) is an endangered subspecies endemic to Key Largo, Florida, USA, that has undergone substantial declines due to habitat loss and predation by invasive predators. We leveraged data from a camera trap monitoring grid at supplemental woodrat nest structures to survey bird communities to evaluate the role of woodrat nest use and stick-nest building …

Upregulation Of Cell Surface Glycoproteins In Correlation With Kshv Lana In The Kaposi Sarcoma Tumor Microenvironment, Sara R. Privatt, Owen Ngalamika, Jianshui Zhang, Qinsheng Li, Charles Wood, John T. West

School of Biological Sciences: Faculty Publications

HIV-associated epidemic Kaposi sarcoma (EpKS) remains one of the most prevalent cancers in sub-Saharan Africa despite the widespread uptake of anti-retroviral therapy and HIV-1 suppression. In an effort to define potential therapeutic targets against KS tumors, we analyzed previously published KS bulk tumor transcriptomics to identify cell surface biomarkers. In addition to upregulated gene expression (>6-fold) in the EpKS tumor microenvironment, biomarkers were selected for correlation with KSHV latency-associated nuclear antigen (LANA) expression. The cell surface glycoprotein genes identified were KDR, FLT4, ADAM12, UNC5A, ZP2, and OX40, as well as the endothelial lineage determinants Prox-1 and CD34. Each protein …

Application Of A Novel Lytic Jerseyvirus Phage Lpsent1 For The Biological Control Of The Multidrug-Resistant Salmonella Enteritidis In Foods, Rashad R. Al-Hindi, Mona G. Alharbi, Ibrahim Alotibi, Sheren A. Azhari, Khloud M. Algothmi, Ahmed Esmael

Nebraska Center for Virology: Faculty Publications

Non-typhoidal Salmonella is the tremendously predominant source of acquired foodborne infection in humans, causing salmonellosis which is a global threat to the healthcare system. This threat is even worse when it is combined with the incidence of multidrug-resistant Salmonella strains. Bacteriophage therapy has been proposed as a promising potential candidate to control a diversity of foodborne infective bacteria. The objective of this study designed to isolate and characterize lytic phages infecting zoonotic multi-drug resistant and strong biofilm producer Salmonella enterica serovar Enteritidis EG.SmE1 and then apply the isolated phage/s as a biocontrol agent against infections in ready-to-eat food articles including …

Evaluating Draft Epa Emissions Models For Laying Hen Facilities, Yijie Xiong, Guoming Li, Brett C. Ramirez, Robert T. Burns, Richard S. Gates

Department of Animal Science: Faculty Publications

In August 2021, the U.S. Environmental Protection Agency (EPA) released draft models to estimate daily NH₃, H₂S, PM₁₀, PM_2.5, and TSP emissions from egg-layer houses (high-rise and manure-belt) and manure storage using inputs of daily mean ambient temperature, relative humidity (RH), and hen inventory. These models were developed from refined datasets generated by the National Air Emissions Monitoring Study fieldwork completed in 2009. Notably, they do not include data for cage-free housing. Currently, 66% of U.S. laying hens are housed in cages; thus, these models, if adopted, will have a substantial impact …

A Diverse Flea (Siphonaptera) Assemblage From The Small Mammals Of Central New Mexico, Dianne E. Peterson, Jonathan L. Dunnum, Schuyler W. Liphart, Karen A. Boegler, Ralph P. Eckerlin, Eric P. Hoberg, Martha O. Perez-Arriaga, Katrina Derieg, David Garnand, Mariel L. Campbell, Joseph A. Cook

MANTER: Journal of Parasite Biodiversity

The geographical ranges of many mammals and their associated parasites are dynamic. Comprehensive documentation of these communities over time provides a foundation for interpreting how changing environmental conditions, driven by accelerating climate change, other anthropogenic disturbances, and natural events, may influence host-parasite interactions. Fleas (Order Siphonaptera) are obligate, hematophagous parasites of birds and mammals with medical interest because of their role in transmitting pathogens. From 2016 to 2019, we sampled the small mammal and associated flea communities in El Malpais National Conservation Area (El Malpais) in Cibola County, New Mexico. Among 898 mammalian specimens, 925 fleas representing 29 species were …

Sustainability Club, Brianna Bernhardt, Marissa Lindemann

Honors Program: Expanded Learning Clubs

An environmental and agricultural-based club focusing on sustainability. Results in a cleaner school environment, knowledge about various sustainable practices, art projects, and an appreciation of nature.

Stem Club, Rohan Tatineni, Kevin Prososki

Honors Program: Expanded Learning Clubs

The goal of STEM club is to teach the students various basic scientific principles and relate them to experiments and the real world. In addition, the scientific method will be taught and be a main focus of the club along with the engineering design process.

Fish & Wildlife News: Spring 2023

Fish and Wildlife News

No abstract provided.

Gsl Researcher (Spring 2023)

GSL Researcher

GSL Researcher (Spring 2023)

Greetings

People

Range and pasture update

Ranch update

Technical notes

Highlighted research

Nebraska ranch practicum

Recent publications

Dealing With “Humanewashing” Claims: Vital Farms’ Crucial Next Step, Bryce Janke, Cole Johnson, Kieran Mcwilliams, Justin Morrow, Isabelle Schmidt

B-Corp Case Studies by UNL Raikes Seniors

Vital Farms prides itself in their ethical and environmentally-friendly production of pasture-raised eggs, butter, and ghee. A popular tagline for the company is “keeping it bullsh*t-free.” This is core to their company mission and values. However, they are currently facing a PETA class action lawsuit for potential humanewashing and greenwashing, essentially false advertising. So, what happens when the Certified B-Corporation is under attack for the very thing that sets it apart from its competition? Is it possible to rebuild trust with the public and regain ethical standing after bad PR? What are humanewashing and greenwashing, and is Vital Farms guilty?

Purpose, Place, And People: How The Pandemic Helped Foster Open And Inclusive Course Design, Benjamin K. Haywood, Diane E. Boyd, John A. Mcarthur

To Improve the Academy: A Journal of Educational Development

In the spring of 2020, many institutions of higher education rapidly adopted new models of course delivery to support the ongoing need for instructional flexibility in response to the SARS-CoV-2 pandemic. This article discusses how the transition to a flexible instructional model at Furman University created space for faculty to consider the value of intentional learning environment design to meet students where they are both literally and figuratively. Reporting on a course planning framework focused on the alignment of learning outcomes, learning space, and considerations of student engagement and accessibility, this article reviews data from a faculty survey on pedagogical …

Wpa News 120 (2023), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (Spring 2023), number 120

Published by the World Pheasant Association

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Volume 2, Edition 4, April 2023, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

Contents

K-12 curriculum and resources

Career exploration: pathways for students who like animals and insects

CASNR six interest areas: analytical investigators, health systems innovators, food systems futurists, environmental stewards, entrepreneurial designers, community leaders

Analytical investigators careers spotlight: UNL statistics, forensic science, agricultural economics

Teacher and student opportunities: applied science program for flexible schooling, student summer opportunities, young Nebraska scientists summer camps

White Coat Color Of A Black Angus Calf Attributed To An Occurrence Of The Delr217 Variant Of Mitf, Jessica Lynn Petersen, Renae L. Sieck, David J. Steffen

Department of Animal Science: Faculty Publications

A white calf, with minimal pigmented markings, was born to two registered Black Angus parents. Given the possibility of an unknown recessive or de novo dominant mutation, whole-genome sequencing was conducted on the trio of individuals. A 3-bp in-frame deletion in MITF was identified; this mutation was unique to the calf but identical to the delR217 variant reported in both humans and murine models of Waardenburg syndrome type 2A and Tietz syndrome. Given the coat color phenotype and identity of the mutation, our data support that this calf represents the first instance of this recurring MITF mutation in cattle.

Unl Emeriti And Retirees Association Newsletter, Spring 2023

UNL Emeriti and Retirees Association: Newsletters and Other Materials

University of Nebraska-Lincoln Emeriti and Retirees Association Newsletter, Spring 2023

Experimentation On Nebraska Farms For Sustaining Soil Health Management, Fernanda Souza Krupek

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Soil health management practices have increasingly been promoted across US agroecosystems to address many interrelated environmental and economic food system challenges. Sustaining conservation behavior – through farmer’s adoption and continued use of practices – is key for achieving many soil health-related intended social-ecological benefits. Using a range of scientific methods, from lab-based experiments to on-farm research to farmer interviews, the overall objective of this dissertation research was to explore soil and human dimension considerations to design farming and knowledge transfer systems for sustaining soil health management in the US Midwest. In a multivariate analysis of ten on-farm research sites, we …

Characterization Of Physical And Biochemical Traits In Wheat And Corn Plants Using High Throughput Image Analysis, Kantilata Thapa

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Plant phenotyping has been recognized as a rapidly growing field of research due to the labor-intensive, destructive, and time-consuming nature of traditional phenotyping methods. These phenotyping bottlenecks can be addressed by advancements in image-based phenotyping like RGB and hyperspectral imaging for the assessment of plant traits important for breeding purposes. This study aims (1) to characterize the physical and biochemical traits of wheat and corn plants using RGB and hyperspectral imaging in the greenhouse, and (2) to estimate leaf nitrogen (N), phosphorus (P), and potassium (K) content using hyperspectral imaging and an analytical spectral device (ASD spectrometer) and compare the …

Extraction And Analytical Method For N-Nitrosoatrazine Using Liquid Chromatography Paired With Tandem Mass Spectrometry, Carolyn Billings

Honors Program: Senior Projects (Public)

Atrazine is an herbicide used in the United States to control weeds. When combined with nitrite, atrazine can be nitrosated to form N-nitrosoatrazine (NNAT) in vivo and in vitro under acidic conditions. Atrazine can also be metabolized to atrazine mercapturate, deethylatrazine (DEA), didealkylatrazine (DDA), and deisopropylatrazine (DIA). Atrazine is monitored in drinking water due to its possible carcinogenicity. The objective of this study was to develop extraction and analytical methods for NNAT, atrazine, and atrazine metabolites. An instrument detection limit, extraction methods comparison, and extraction method detection limit were determined. To compare methods, Fischer 344 rat tissue samples were …

The Effects Of Exercise On The Lipid Profile Of Extracellular Vesicles, Tesha Kerr

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

We sought to ascertain the effect of different types of exercise (aerobic training and resistance training) on the lipid profile of extracellular vesicles. Thus, we had participants perform bouts of AT and RT and rest (control) and collected blood samples from them immediately after (timepoint 0) and 30 minutes after (timepoint 30) exercise. Small extracellular vesicles (sEVs) were isolated from plasma of participants using cushioned-density gradient ultracentrifugation (C-DGUC). Small EV size, morphology, and protein markers were determined using nanoparticle tracking analysis, transmission electron microscopy, and western blot, respectively. Additionally, we performed untargeted lipidomics on the sEV isolate. We found that …

Wakara's Waterscapes: Storytelling, Cartography, And Rhetorical Sovereignty On The Shores Of The Green River, Abbey O'Brien

Honors Program: Senior Projects (Public)

In the mid nineteenth-century, Wakara, a prominent Ute leader, witnessed the invasion of his homeland by Mormon settlers and mountain-men. He met the scouts and explorers who were sent out to examine the land and waterscapes, and who drew maps along their way. It was those same maps which were eventually used as tools to justify colonial expansion all across the Utah territories, Wakara’s home. But Wakara resisted. Employing his understandings of the roles that cartography and the written word played in Mormon and settler discourse, Wakara created his own maps in order to assert his Indigenous authority over the …

Agen/Bsen 112 Final Project: Moving And Temperament Of Cattle, Elly Vo, Taetum Baxa, Ved Patel, Brady Giles, Tami M. Brown-Brandl, Rossana Villa-Rojas

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Cattle movement and weather may affect the body temperature of cows which in turn affects their natural behavior and can influence their metabolism. Cattle take several days to resume their normal eating patterns after being overheated, and that can affect dairy production. This project was assigned to study the effect of temperament (calm vs excitable) and evaporative cooling on the body temperature of moving animals to optimize the environmental conditions around the cattle and consequently, dairy production.

The project began with processing of a data set from Tami Brown-Brandl who was the client/adviser for the group. The data contained the …

Drinking Water Contaminants: Bacteria, Bruce I. Dvorak, Becky O. Schuerman

Department of Civil and Environmental Engineering: Faculty Publications

Testing drinking water for fecal bacteria or Escherichia coli (E. coli) is commonly used as an indicator of the presence of pathogenic microorganisms. The presence of pathogenic (disease-causing) microorganisms is a concern when considering the safety of drinking water. Pathogenic microorganisms can cause flu-like systems, intestinal infections, dysentery, hepatitis, typhoid fever, cholera, and other illnesses.

Highly Pathogenic Avian Influenza A(H5n1) Virus Outbreak In New England Seals, United States, Wendy Puryear, Kaitlin Sawatzki, Nichola Hill, Alexa Foss, Jonathon J. Stone, Lynda Doughty, Dominique Walk, Katie Gilbert, Maureen Murray, Elena Cox, Priya Patel, Zak Mertz, Stephanie Ellis, Jennifer Taylor, Deborah Fauquier, Ainsley Smith, Robert A. Digiovanni, Adriana Van De Guchte, Ana Silvia Gonzalez-Reiche, Zain Khalil, Harm Van Bakel, Mia K. Torchetti, Kristina Lantz, Julianna B. Lenoch, Jonathan Runstadler

United States Department of Agriculture Wildlife Services: Staff Publications

We report the spillover of highly pathogenic avian influenza A(H5N1) into marine mammals in the northeastern United States, coincident with H5N1 in sympatric wild birds. Our data indicate monitoring both wild coastal birds and marine mammals will be critical to determine pandemic potential of influenza A viruses.

Simple Darwin Core For Non-Biologists Primer, Megan N. O'Donnell, Leslie M. Delserone

University of Nebraska-Lincoln Libraries: Faculty Publications

This primer focuses on Simple DwC (http://rs.tdwg.org/dwc/terms/simple/), a “mechanism used to share biodiversity information using the simplest methods and structure” (Darwin Core Task Group, 2014). With Simple DwC, the DwC schema is applied to a single flat file (i.e., table or spreadsheet). Because it is a self-contained data set that can be opened, edited, and analyzed using a wide variety of software, Simple DwC is easier to implement than other forms of DwC that use XML, RDF, or relational databases. The primer’s goal is to assist a curator presented with a data set structured in Simple DwC, or …

A Device For Measuring Acoustic Output Intensity Of Transcranial Doppler Ultrasound Transducers For Comparison With Fda Regulations, Sarah Altman, Gregory Bashford, Benjamin Hage

Honors Program: Senior Projects (Public)

Transcranial Doppler Ultrasonography (TCD) is a non-invasive methodology which can evaluate cerebral blood flow velocity in real time. Single-element focused circular transducers placed on the scalp produce ultrasound waves capable of penetrating the skull with minimal aberration, enabling measurement of the Doppler shift. As such, TCD can measure blood flow velocity in the internal carotid and vertebral arteries, as well as the arteries of the Circle of Willis: the Anterior Cerebral (ACA), Posterior Cerebral (PCA), and Middle Cerebral Arteries (MCA). Intracranial aneurysm and ischemic stroke are serious conditions in which ballooning or occlusion of cerebral vessels cause insufficient perfusion, leading …