Digital Commons Network^™

Initial Experience With An Electron Flash Research Extension (Flex) For The Clinac System, Kyuhak Oh, Kyle J. Gallagher, Megan Hyun, Diane Schott, Sarah Wisnoskie, Yu Lei, Samuel Hendley, Jeffrey Wong, Shuo Wang, Brendan Graff, Christopher Jenkins, Frank Rutar, Md Ahmed, Joshua Mcneur, Jeffrey Taylor, Marty Schmidt, Lasitha Senadheera, Wendy Smith, Subodh M. Lele, Ran Dai, Dong Jianghu (James), Y Yan, Su-Min Zhou

Department of Physics and Astronomy: Faculty Publications

Purpose: Radiotherapy delivered at ultra-high-dose-rates (≥40 Gy/s), that is, FLASH, has the potential to effectively widen the therapeutic window and considerably improve the care of cancer patients. The underlying mechanism of the FLASH effect is not well understood, and commercial systems capable of delivering such dose rates are scarce. The purpose of this study was to perform the initial acceptance and commissioning tests of an electron FLASH research product for preclinical studies.

Methods: A linear accelerator (Clinac 23EX) was modified to include a nonclinical FLASH research extension (the Clinac-FLEX system) by Varian, a Siemens Healthineers company (Palo Alto, …

Leucocytozoon Cariamae N. Sp. And Haemoproteus Pulcher Coinfection In Cariama Cristata (Aves: Cariamiformes): First Mitochondrial Genome Analysis And Morphological Description Of A Leucocytozoid In Brazil, Lis Marques De C. Vieira, Pedro Henrique O. Pereira, Daniel Ambrózio Da Rocha Vilela, Irène Landau, M. Andreína Pacheco, Ananias A. Escalante, Francisco C. Ferreira, Érika Martins Braga

Harold W. Manter Laboratory of Parasitology: Library Materials

The distribution of avian haemosporidians of the genus Leucocytozoon in the Neotropics remains poorly understood. Recent studies confirmed their presence in the region using molecular techniques alone, but evidence for gametocytes and data on putative competent hosts for Leucocytozoon are still lacking outside highland areas. We combined morphological and molecular data to characterize a new Leucocytozoon species infecting a non-migratory red-legged seriema (Cariama cristata), the first report of a competent host for Leucocytozoon in Brazil. Leucocytozoon cariamae n. sp. is distinguished from the Leucocytozoon fringillinarum group by its microgametocytes that are not strongly appressed to the host cell …

Evaluation Of Native Plant Communities On Nebraska Game And Parks Commission State Parks, Robert F. Steinauer

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

This report evaluates the Native Plant Communities on Nebraska Game and Parks Commission State Parks East Region, also known as Region 3, which includes much of northeast and extreme eastern Nebraska, including portions of the lower Niobrara, Elkhorn and Platte Rivers and nearly the entire east border formed by the Missouri River. Fifteen state-listed threatened and endangered species have been recorded within the boundaries of Region 3 within the last twenty years. Several of these occupy habitats within major river channels.

Surveyed areas which contained habitat for legally protected species include Dead Timber State Recreation Area, Indian Cave State Park, …

Targeted Sequencing Of The Panicum Miliaceum Gene Space And Genotyping Of Variant Sites From Population Genetics Studies, Combined In A Single Assay, As A Tool For Broomcorn Millet Assisted Breeding, Gabriele Magris, Serena Foria, Silvano Ciani, Dipak K. Santra, Ombretta Polenghi, Virna Cerne, Michele Morgante, Gabriele Di Gaspero

Department of Agronomy and Horticulture: Faculty Publications

Broomcorn millet (Panicum miliaceum L.) is a prehistorical cereal, today cultivated as a minor crop with low yields but with a renewed interest for its high water use effciency and gluten-free grains. To reverse the downward trend in broomcorn millet cultivation, the crop needs genetic improvement and creation of novel genetic variation to increase productivity. In order to facilitate genomics-assisted breeding, we designed a reduced representation genome-sequencing assay that investigates 1.8% of the nuclear DNA in a targeted and reproducible way, with an intensity of genomic sampling that is a direct function of local recombination rate. We used this …

The Devil You Know And The Devil You Don’T: Current Status And Challenges Of Bovine Tuberculosis Eradication In The United States, Daniel J. O'Brien, Tyler C. Thacker, Liliana C.M. Salvador, Anthony G. Duffiney, Suelee Robbe‑Austerman, Mark S. Camacho, Jason E. Lombard, Mitchell V. Palmer

United States Department of Agriculture Wildlife Services: Staff Publications

Having entered into its second century, the eradication program for bovine tuberculosis (bTB, caused by Mycobacterium bovis) in the United States of America occupies a position both enviable and daunting. Excepting four counties in Michigan comprising only 6109 km2 (0.06% of US land area) classified as Modified Accredited, as of April 2022 the entire country was considered Accredited Free of bTB by the US Department of Agriculture for cattle and bison. On the surface, the now well-described circumstances of endemic bTB in Michigan, where white-tailed deer (Odocoileus virginianus) serve as a free-ranging wildlife maintenance host, may appear to be …

Dna Methylation Networks Underlying Mammalian Traits, Amin Haghani

Department of Animal Science: Faculty Publications

INTRODUCTION: Comparative epigenomics is an emerging field that combines epigenetic signatures with phylogenetic relationships to elucidate species characteristics such as maximum life span. For this study, we generated cytosine DNA methylation (DNAm) profiles (n = 15,456) from 348mammalian species using amethylation array platform that targets highly conserved cytosines.

RATIONALE: Nature has evolved mammalian species of greatly differing life spans. To resolve the relationship ofDNAmwith maximum life span and phylogeny, we performed a largescale cross-species unsupervised analysis. Comparative studies in many species enables the identification of epigenetic correlates of maximum life span and other traits.

RESULTS: We …

Universal Dna Methylation Age Across Mammalian Tissues, A. T. Lu, Jessica Lynn Petersen, Steve Horvath, Et Al.

Department of Animal Science: Faculty Publications

Aging, often considered a result of random cellular damage, can be accurately estimated using DNA methylation profiles, the foundation of pan-tissue epigenetic clocks. Here, we demonstrate the development of universal pan-mammalian clocks, using 11,754 methylation arrays from our Mammalian Methylation Consortium, which encompass 59 tissue types across 185 mammalian species. These predictive models estimate mammalian tissue age with high accuracy (r > 0.96). Age deviations correlate with human mortality risk, mouse somatotropic axis mutations and caloric restriction. We identified specific cytosines with methylation levels that change with age across numerous species. These sites, highly enriched in polycomb repressive complex 2-binding locations, …

Indigenous Lakadong Turmeric Of Meghalaya And Its Future Prospects, Manjit Kumar Ray, Dipak K. Santra, Piyush Kumar Mishra, Saurav Das

Department of Agronomy and Horticulture: Faculty Publications

Turmeric (Curcuma longa) has long been used in traditional Indian medicine. India accounts for 80% of total global turmeric production. Lakadong turmeric gets its name from the tiny village of Lakadong, which is located in the foothills of the Jaintia Hills in Meghalaya, India. It is known for having a high curcumin content of more than 7%, as opposed to 2 - 4% in regular varieties. The tribes of this region brought Lakadong turmeric from the forest and domesticated it for medicinal purposes centuries ago. Growth in local coal industries and a gradual decline in the market have …

Functional Connectivity Varies Across Scales In A Fragmented Landscape, Kate I. T. Bird, Daniel R. Uden, Craig R. Allen

School of Natural Resources: Faculty Publications

Species of different sizes interact with the landscape differently because ecological structure varies with scale, as do species movement capabilities and habitat requirements. As such, landscape connectivity is dependent upon the scale at which an animal interacts with its environment. Analyses of landscape connectivity must incorporate ecologically relevant scales to address scale-specific differences. Many evaluations of landscape connectivity utilize incrementally increasing buffer distances or other arbitrary spatial delineations as scales of analysis. Instead, we used a mammalian body mass discontinuity analysis to objectively identify scales in the Central Platte River Valley (CPRV) of Nebraska, U.S.A. We implemented a graph-theoretic network …

Machine Learning Techniques To Predict The Air Quality Using Meteorological Data In Two Urban Areas In Sri Lanka, Lakindu Mampitiya, Namal Rathnayake, Lee P. Leon, Vishwanadham Mandala, Hazi Md. Azamathulla, Sherly Shelton, Yukinobu Hoshino, Upaka Rathnayake

Department of Earth and Atmospheric Sciences: Faculty Publications

The effect of bad air quality on human health is a well-known risk. Annual health costs have significantly been increased in many countries due to adverse air quality. Therefore, forecasting air quality-measuring parameters in highly impacted areas is essential to enhance the quality of life. Though this forecasting is usual in many countries, Sri Lanka is far behind the state-of-the-art. The country has increasingly reported adverse air quality levels with ongoing industrialization in urban areas. Therefore, this research study, for the first time, mainly focuses on forecasting the PM₁₀ values of the air quality for the two urbanized areas …

Ten Questions Concerning Smart And Healthy Built Environments For Older Adults, Sanjay Kumar, Samuel H. Underwood, Julie L. Masters, Natalie A. Manley, Iason Konstantzos, Josephine Lau, Ryan Haller, Lily M. Wang

Durham School of Architectural Engineering and Construction: Faculty Publications

In the past decade, the term ‘global aging’ has gained much attention among researchers, policymakers, civil societies, and governments worldwide. The continuous decline in fertility rates and increased life expectancy have resulted in an ongoing demographic transition where the share of adults aged 65 or older is increasing and outnumbering children younger than five years. In the United States and other developed countries, this rapid demographic transition is expected to continue to strain the existing health infrastructure as it becomes increasingly challenging to ensure healthy living environments for older adults. The ten questions and answers in this paper have been …

Catalog Of The World Species Of Trichodesma Leconte, 1861 (Coleoptera: Ptinidae) And Associated Genera, Kyle E. Schnepp

Insecta Mundi

A catalog of the species of Trichodesma LeConte (Coleoptera: Ptinidae) for the world is provided, including synonyms and misspellings. Six additional genera, Anobiopsis Fall, Belemia Español, Nanodesma Zahradník, Nicobium LeConte, Trichobiopsis White, and Trichodesmina Español, are also cataloged for com­pleteness. The species name, author, year, and page number of description are given for each species, as well as references for combinations by different authors. The type depository is given when known, and followed by a “?” when unknown but suspected, and a general distribution is given by region of the world followed by country. Authorship of the genus Trichodesma in …

Characterizing Feedlot Feed Using Depth Cameras And Imaging Technology, Jean Niwenshuti

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Imaging technology is a growing field that provides solutions in many areas from manufacturing to agriculture. Through previous research, imaging technologies have been studied in livestock farming to monitor the animal’s health and welfare in the production process. However, the feedlot industry is still behind in validating the feasibility to use some of these technologies nor to adopt such technologies to address challenges the industry is facing, such as lack of skilled labor.This work proposes using novel imaging methods to identify feed types and estimate the amount of feed remaining in a typical Midwestern feedlot feed bunk. These methods have …

Flex-Ro: Development And Testing Of A Semi-Autonomous Robotic Planter, Ian Tempelmeyer

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The need for more crop production with minimal negative environmental impact is a continuing pursuit around the world. As the population continues to increase at an exponential rate, the demand for food, fiber, fuel, and energy also increases. Robotics and automation are seen as one of the useful technology tools to increase field productivity. This work specifically focuses on addressing labor challenges for one of the most important field operations, planting. A robotic planter unit was designed as part of this work that mounted on unmanned ground vehicle (UGV) to perform autonomous planting operation in a 1 ha (2.5 acre) …

Auricularia Auricula Polysaccharides Attenuate Obesity In Mice Through Gut Commensal Papillibacter Cinnamivorans, Xin Zong, Hao Zhang, Luoyi Zhu, Edward C. Deehan, Jie Fu, Yizhen Wang, Mingliang Jin

Department of Nutrition and Health Sciences: Faculty Publications

Introduction: Auricularia auricula is a well-known traditional edible and medical fungus with high nutritional and pharmacological values, as well as metabolic and immunoregulatory properties. Nondigestible fermentable polysaccharides are identified as primary bioactive constituents of Auricularia auricula extracts. However, the exact mechanisms underlying the effects of Auricularia auricula polysaccharides (AAP) on obesity and related metabolic endpoints, including the role of the gut microbiota, remain insufficiently understood.

Methods: The effects of AAP on obesity were assessed within high-fat diet (HFD)-based mice through obesity trait analysis and metabolomic profiling. To determine the mechanistic role of the gut microbiota in observed anti-obesogenic …

How Can Windbreaks Protect Livestock From The Cold?

Working Trees (USDA-NAC)

Windbreaks can protect livestock from the cold resulting in improved feeding efficiency, increased milk production, improved overall animal health, increased forage yield, and other benefits.

Nebline, August/September 2023

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Understanding the “WHY” of Challenging Behavior in Young Children Can Help With Strategies by Hayley Jackson

5 Reasons to Support Your Farmers Market by Tara Dunker

Recipes of the Month: Chocolate Zucchini Muffins and Fresh Salsa by Kayla Colgrove

Time for Fall Lawn Seeding by Sarah Browning

Garden Guide: Things to Do This Month

Using Water Resources More Efficiently by Becky Schuerman

Parasitoids: Nature’s Biocontrol by Kait Chapman

Lancaster County Super Fair, Aug. 3-12, 2023

Webinars Addressing Biting Pests in Early Childhood and School Settings

Ag Landlord/Tenant Workshop

4-H Announcements for 4-H'ers or 4-H Volunteers

Heart of 4-H Volunteer Award: …

Influence Of Overcooking On Food Digestibility And In Vitro Fermentation, Wensheng Ding

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Areas of char or overcooking commonly appear in foods people consume. It has been reported that overcooked food is harmful to human health. However, little research exists on the effect of overcooking on in vitro protein and starch digestibility and gut microbial fermentation. This study aimed to reveal the connection between overcooking and in vitro protein and starch digestibility and gut microbial fermentation. In vitro protein digestibility of an overcooked ground beef patty was almost half that of a standard cooked sample (27 ± 2% versus 48 ± 6%, respectively; p = 0.02). Whole wheat bread protein digestibility was also …

Examining Potential Trade-Offs Between Pest Management And Pollination To Sunflower Production In Nebraska, Luis Ochoa Cadena

Department of Entomology: Dissertations, Theses, and Student Research

Sunflower (Helianthus annuus) is cultivated for oil and seed production worldwide, and the United States produces 2.5% of the world’s oilseed sunflower primarily grown in the Great Plains. In 2022, oilseed and confectionary U.S. sunflower production increased by 48 and 44%, respectively, compared to last year's records. However, sunflower production can be seriously compromised by insect pests. The red sunflower seed weevil (Smicronyx fulvus LeConte) is considered the most serious pests in North America and causes severe economic damage yearly. On the other hand, native bees contribute substantially to sunflower yield. Currently, insecticide applications are the primary tool to suppress …

Assessment Of Integrated Nutrient Management Practices On Soil Health And Nitrogen Efficiency In Cropping Systems, María José Oviedo Ventura

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Groundwater quality in Nebraska is threatened by non-point source contributions of nitrate-nitrogen, primarily by agricultural systems. Intensive crop and livestock production that neglects the value of manure in crop fertility plans can contribute to soil degradation and natural resource impairment. The purpose of this study is to evaluate opportunities to improve agricultural nutrient management throughout Nebraska by quantifying opportunities for manure distribution, evaluating soil health implications of organic and inorganic soil amendments, and assessing nutrient management practices focused on reducing nitrate leaching in agricultural soils.

This study described in Chapter 2 provides a spatial illustration of nitrogen and phosphorus balances …

Wild Bee Utilization Of Engineered Pollinator Habitat In Eastern Nebraska Agroecosystems, Shianne Lindsay

Department of Entomology: Dissertations, Theses, and Student Research

Habitat loss is a leading cause of biodiversity decline globally. Further, loss of natural and semi-natural habitat is a major factor contributing to declines in wild pollinator populations. Among intensive agroecosystems in the Midwestern United States, corn, soy, and small grain fields dominate the landscape and leave limited space available for forb-rich habitat that supports diverse pollinator assemblages to exist and thrive. There are numerous barriers and challenges to integrating pollinator habitat into such agroecosystems and quantitative assessments of impacts on pollinator communities are largely lacking. The establishment of high-quality pollinator habitat can provide both high flower abundance and species …

A Thematic Analysis Exploring The Narratives Of Growing Up As A Second-Generation Survivor Of The 1994 Genocide Against The Tutsi In Rwanda And The Presence Of Generative Commitment, Victor Mpore

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

The scientific analysis of narratives from survivors of a genocide like the 1994 genocide against the Tutsi has provided an understanding on the survivors experiences during and after the genocide. Most narrative research on the 1994 genocide against the Tutsi has however primarily focused on the survivors and not their children. This study focuses on the second-generation survivors as a significant portion of the Rwandan population was born after the genocide. Drawing on the narrative approach, this study thematically analyzed narratives the second-generation survivors of the 1994 genocide against the Tutsi have about: a) their childhood and young adulthood in …

Prairie Protector: Student Development Of Systems Thinking Habits In The Context Of Agroecosystems, Erin Ingram, Jennifer Keshwani

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Introduction: The Great Plains ecosystem offers an opportunity for young people to gain knowledge about intricate systems through practical learning. The spread of woody plants into grasslands, known as the “Green Glacier,” poses a risk to biodiversity and animal production. Although effective management strategies exist, some land managers are reluctant to use them. It is critical to cultivate a scientifically literate population that can think systematically and make informed decisions based on STEM principles to address such complex agroecosystem problems.

Methods: In this study, semi-structured focus group discussions with high school students were analyzed to determine whether Prairie …

Leveraging Eco-Evolutionary Models For Gene Drive Risk Assessment, Matthew A. Combs, Andrew J. Golnar, Justin M. Overcash, Alun L. Lloyd, Keith R. Hayes, David A. O'Brochta, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

Engineered gene drives create potential for both widespread benefits and irreversible harms to ecosystems. CRISPR-based systems of allelic conversion have rapidly accelerated gene drive research across diverse taxa, putting field trials and their necessary risk assessments on the horizon. Dynamic processbased models provide flexible quantitative platforms to predict gene drive outcomes in the context of system-specific ecological and evolutionary features. Here, we synthesize gene drive dynamic modeling studies to highlight research trends, knowledge gaps, and emergent principles, organized around their genetic, demographic, spatial, environmental, and implementation features. We identify the phenomena that most significantly influence model predictions, discuss limitations of …

Dry-Aged Beef Flavor Development, And The Effect Of High Levels Of Vitamin-E On Beef Color Stability, Nicolas Herrera

Department of Animal Science: Dissertations, Theses, and Student Research

Palatability and consumer purchasing are essential factors of fresh meat. To meet consumer expectations during purchasing and consumption, fresh beef must maintain desirable cherry-red color at retail and achieve desirable cooked flavor. Dry-aged beef popularity has grown, and identifying methods to ensure quality dry-aged meat is necessary to maintain demand. To understand dry-aged palatability, this dissertation addressed effects of moisture loss and aging time, fundamental factors of dry-aged beef, using bone-in and boneless strip loins. Study two investigated flavor development using cookery methods and internal temperatures in boneless and bone-in dry-aged beef. In study one, increased dry-aging time (creation effect) …

Identification And Assessment Of Lameness In Sows Through The Utilization Of NuTrack And Gaitfour Systems, Lexi M. Ostrand

Department of Animal Science: Dissertations, Theses, and Student Research

Lameness is a financial concern for producers and a welfare concern for the animals. Utilizing both the GAITFour and NUtrack systems, gilts’ natural tendencies and gait were analyzed to better understand the onset of lameness and what indicators can be identified in a commercial setting. Beginning at 20 weeks of age, video on replacement gilts was collected for 9 consecutive days and processed using the NUtrack System to identify individual gilts’ distance travelled (m), avg speed (m/s), angle rotated (degrees), and time standing (s), sitting (s), eating (s), and laying (s). NUtrack is a deep learning-based multi-object …

Consequences Of Cow–Calf Production With Limited Perennial Forage Grazing, Hannah Fae Speer

Department of Animal Science: Dissertations, Theses, and Student Research

Conversion of perennial grasslands to cropland and rising land prices prompts a need to evaluate alternative beef cow-calf production systems. A 3-yr study was conducted comparing beef cow-calf performance between two August-calving cow systems: perennial forage and corn residue grazing; or summer drylot, fall grazing of late-summer-planted cover crop, and corn residue grazing. Cow body condition score differed between systems, but condition of cows in both systems never fell below adequate. There were no differences in pregnancy rates between systems. Calf weaning weights differed between systems each year; differences are attributed to feedstuff quantity, quality, and type available to calves …

Utilizing Mitotalens To Investigate Large Repeats In The Arabidopsis Thaliana Mitochondrial Genome, Cambelle Johnson

Honors Program: Senior Projects (Public)

Mitochondria are a silly place to store the genetic code. In spite of the ever-present threat of oxidative DNA damage, plant mitochondrial genomes are expansive in size and include noncoding repeat sequences, a small number of which are greater than 1kilobase (kb), known hereafter as “large repeats” (LR). This theme of one or more large repeat pairs is shared across most flowering plants, lending to the belief that they somehow contribute to the plant’s fitness. These repeats exhibit high rates of rearrangement and recombination. Studying repeats in vivo could have large scale implications in hybrid crop production enhancement including eliminating …

Ecology And Host Manipulation By An Egg-Larva Parasitoid Of Chrysodeixis Includens (Lepidoptera: Noctuidae), Daniel V.C. Neves, Renata R. Pereira, Julia N.D. Campos, Rodrigo S. Ramos, Paulo A.S. Junior, Daiane G. Carmo, Marcelo C. Picanço

Department of Entomology: Faculty Publications

Parasitoids are among the main natural enemies of crop pests. Copidosoma truncatellum (Hymenoptera: Encyrtidae) is a parasitoid of Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae: Plusiinae), which is an important pest of soybean, bean, cotton, sunflower, tomato and potato. Copidosoma are parasitoids of lepidopteran egg-larva, especially those of the subfamily Plusiinae. The embryonic development of the Copidosoma parasitoid begins in the lepidopteran egg, and this development extends to the beginning of the host larval stage of the parasitized lepidopteran. However, the rate of parasitism is a complex ecological relationship affected by climatic elements and age of the host. Thus, the present study …

Loss Of Flockmates Weakens Winter Site Fidelity In Golden-Crowned Sparrows (Zonotrichia Atricapilla), Anastasia E. Madsen, Bruce E. Lyon, Alexis S. Chaine, Theadora A. Block, Daizaburo Shizuka

School of Biological Sciences: Faculty Publications

Animal social interactions have an intrinsic spatial basis as many of these interactions occur in spatial proximity. This presents a dilemma when determining causality: Do individuals interact socially because they happen to share space, or do they share space because they are socially linked? We present a method that uses demographic turnover events as a natural experiment to investigate the links between social associations and space use in the context of interannual winter site fidelity in a migratory bird. We previously found that golden-crowned sparrows (Zonotrichia atricapilla) show consistent flocking relationships across years, and that familiarity between individuals …