Digital Commons Network^™

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy Gilbert, University Of Georgia, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern United States, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern US, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean monthly …

Examination Of The Prevalence And Behaviors Of Pathogenic And Spoilage Bacteria At Multiple Stages In The Meat Processing Chain, Samuel C. Watson

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

As long as there is food, there will also be bacteria that are capable of compromising the quality and safety of food. This dissertation aims to study bacteria capable of spoiling meat, causing illness, or both. The literature review summarizes the understanding of several bacterial pathogens, current issues in combating those pathogens in the food supply, and how spoilage Pseudomonas behave in the meat supply. Chapter 2 explored the impact of organic acid treatments on the spoilage microbiome of raw beef during extended storage. While Pseudomonas and Yersinia were both present in significant levels, Lactococcus was determined to be the …

Value-Added Green Processing Of Tomato Waste To Obtain A Stable Free-Flowing Powder Lycopene Formulation Using Supercritical Fluid Technology, Purlen Sezer Okur, Ozan Ciftci

Department of Food Science and Technology: Faculty Publications

The tomato processing industry generates large amounts of waste, which creates disposal problems and negatively impacts the environment. Tomato waste contains lycopene, high-value health- and wellness-promoting bioactive, and it is a potential source of natural lycopene for food and nutraceutical applications. However, isolation of lycopene from its source requires toxic solvents, and lycopene is unstable during storage. The objective of this study was to extract lycopene from tomato waste and to convert it to a shelf-stable and easy-to-use free-flowing powder form using green extraction and particle formation processes based on supercritical carbon dioxide (SC-CO2) technology. Lycopene was extracted using SC-CO2 …

Organic Electronics In Biosensing: A Promising Frontier For Medical And Environmental Applications, Jyoti Bala Kaushal, Pratima Raut, Sanjay Kumar

Durham School of Architectural Engineering and Construction: Faculty Publications

The promising field of organic electronics has ushered in a new era of biosensing technology, thus offering a promising frontier for applications in both medical diagnostics and environmental monitoring. This review paper provides a comprehensive overview of organic electronics’ remarkable progress and potential in biosensing applications. It explores the multifaceted aspects of organic materials and devices, thereby highlighting their unique advantages, such as flexibility, biocompatibility, and low-cost fabrication. The paper delves into the diverse range of biosensors enabled by organic electronics, including electrochemical, optical, piezoelectric, and thermal sensors, thus showcasing their versatility in detecting biomolecules, pathogens, and environmental pollutants. Furthermore, …

A Bio-Economic Modeling Analysis Of Livestock Grazing On Rangelands Utilizing A Supplementary Grazing Resource, Jessica L. Windh

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation is a compilation of 5 chapters culminating in a bioeconomic model of the interactions between an integrated crop-livestock system and native rangeland grazing system. The model is developed using data from the eastern Sandhills of Nebraska, a location where cropping systems of Eastern Nebraska border one of the last intact grasslands remaining in North America.

The first chapter introduces the reader to the systems that will be modeled in the dissertation. In Chapter 2 we used a linear regression to model grassland biomass production as a function of climatic variables; we were unsuccessful in modeling season-long production in …

Janus , A Spliceosome-Associated Protein, Promotes Mirna Biogenesis In Arabidopsis, Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MicroRNAs ( miRNAs ) are important regulators of genes expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex ( MC ) . Here, we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 ( DCL1 ) and SERRATE ( SE ) and directly binds the stem- loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript ( pri-miRNAs ) with MC are reduced. …

Affinity Based Writing Groups: Building Communities Across 9–12 Grade Levels, Melissa Pilakowski

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Writing instruction in secondary classrooms has generally focused on cognitive approaches with feedback and assessment from a limited audience, often only the teacher. However, the burgeoning digital world has made writing an increasingly social activity where individuals can influence a wider audience and engage in dialogue with their readers faster and more efficiently than ever before. This action research aims to study the impacts of online affinity based writing groups that span the 9–12 grade levels, especially in the areas of effort, self-efficacy, and feedback. Data was collected through interviews with participating students and teachers, samples of writing and feedback, …

Supplemental Table 1. Influence Of The Count Of Positive Days On Dmi, Milk Yield, Milk Fat, Milk Protein, And Pregnancy Rate, Addison Carroll, Paul J. Kononoff

Department of Animal Science: Dissertations, Theses, and Student Research

Response

Intercept

Devstarch

Devfat

DevNDF

DevPro

BIC

Comparative Analysis Of Swine Antibody Responses Following Vaccination With Live-Attenuated And Killed African Swine Fever Virus Vaccines, Hung Q. Luong, Huong T. L. Lai, Lam Q. Truong, The N. Nguyen, Hanh D. Vu, Hoa T. Nguyen, Lan T. Nguyen, Trang H. Pham, David Scott Mcvey, Hiep Vu

Nebraska Center for Virology: Faculty Publications

African swine fever virus (ASFV) is circulating in many swine-producing countries, causing significant economic losses. It is observed that pigs experimentally vaccinated with a live-attenuated virus (LAV) but not a killed virus (KV) vaccine develop solid homologous protective immunity. The objective of this study was to comparatively analyze antibody profiles between pigs vaccinated with an LAV vaccine and those vaccinated with a KV vaccine to identify potential markers of vaccineinduced protection. Thirty ASFV seronegative pigs were divided into three groups: Group 1 received a single dose of an experimental LAV, Group 2 received two doses of an experimental KV vaccine, …

Formal Concept Analysis For Image Classification And Machine Learning Models For Anti-Crispr Protein Discovery In Bioinformatics, Minal Khatri

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This study investigates two critical areas in bioinformatics: enhancing transparency in medical image analysis and advancing the discovery of Anti-CRISPR (Acr) proteins, which have potential in developing more precise and controlled CRISPR-Cas gene editing tools. While CNN’s are increasingly applied in critical fields like medical diagnosis, understanding their decision-making process remains a challenge. Although visualization techniques like Saliency maps offer insights into CNN’s decision-making for individual images, they do not explicitly establish a relationship between the high-level features learned by CNN’s and the class labels across dataset. To bridge this gap, Formal Concept Analysis (FCA) framework is leveraged as a …

Center For Grassland Studies, November 2023

Center for Grassland Studies: Newsletters

Contents

Experiential Learning for Grassland Conservation by David Wedin

Center for Grassland Studies Policy Advisory Committee

Director’s Column by David Wedin

New Woody Invasions Ecologist Brings Additional Capacity for Grassland Conservation by Dillon Fogarty

Twidwell Racks-up awards for his work in Grassland Conservation by Geitner Simmons

Platte Basin Timelapse premieres New Film on Nine-Mile Prairie by David Wedin

CRAWL: Update on the Network for Integrated Agricultural Resilience Research by Craig Allen

CGS Outreach by Jerry Volesky

Center for Grassland Studies Awards 19 Scholarships Totaling $17,500 for 2023-2024 by David Wedin

Award winners:

Joseph O. Young Fund = Makenna Anderson, Makennen …

A Memoir Of The Neutzner Family, Hannelore Neutzner Rogers

Archives & Special Collections

A memoir describing the forced expulsion and resettlement the Neutzner family and their relatives from Czechoslovakia to the German Sudetenland during World War II and their later emigration to the United States.

Cell Surface And Plasma Biomarkers Of Kaposi Sarcoma, Sara R. Privatt

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Kaposi sarcoma-associated herpesvirus is the etiological agent of several pathologies including Kaposi sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. Here, we investigated both the tumor microenvironment (TME) in KS lesions as well as plasma from KSHV-infected individuals and how metabolic dysfunction and dysregulated cytokines might have a systemic effect on KS tumorigenesis. Based on previously published KS tumor transcriptomics, one aspect of our investigation involved characterizing cell surface glycoproteins with potential to serve as therapeutic targets against KSHV-infected cells or KS tumors or as biomarkers for disease. Expression patterns of such markers may also suggest the cellular origin of …

Precipitation And Greenness In Pastoral Lands Of East Turkana, Kenya, Paul Akpejeluh

School of Natural Resources: Dissertations, Theses, and Student Research

Pastoralism has long supported livelihoods and provided essential ecosystem services in landscapes of East Africa. Vegetation productivity is central to the functioning of pastoral systems but may be affected by changes in climate and landuse. Vegetation monitoring is important for understanding the effects of global change in pastoral lands; however, it can be time and resource intensive. Remote sensing provides opportunities for efficient multi-scale monitoring of vegetation and climatic drivers. In this thesis, I explore the utility of satellite and UAV remote sensing for monitoring vegetation and precipitation trends and relationships in the East of Lake Turkana Region of northern …

Connecting The Nebraska Water Quality Index To The Aquatic Microbial Community Of The North Platte River Basin, Nebraska, Paula R. Guastello

School of Natural Resources: Dissertations, Theses, and Student Research

The Nebraska Water Quality Index, under development by the Nebraska Department of Environment and Energy, summarizes in a single value eight environmental parameters that have been monitored in Nebraska for nearly 20 years. Water quality parameters including those used in the Nebraska Water Quality Index have been shown in previous studies to impact bacterial growth. As such, this index has the potential to correlate with the freshwater microbial community. Here, I relate the Nebraska Water Quality Index to microbial community composition and structure using 16S rRNA gene amplicon sequence data collected from the North Platte River Basin, Nebraska. This index …

Discursive Struggles Reflected In The Communication Of Conservative Christian Parents And Their Adult Children With Differing Religious Beliefs And Values, Braedon G. Worman

Department of Communication Studies: Dissertations, Theses, and Student Research

Nearly half of American adults no longer believe in their childhood faiths (Pew Research Center, 2015). The steady decline of Christianity could have considerable impacts on family life (Pew Research Center, 2022). From a postmodern critical perspective and guided by Relational Dialectics Theory 2.0, the researcher sought to discern how conservative Christian parents and their adult children with differing religious beliefs and values communicated when they discussed these differences, as well as to identify the discourses that informed and were reflected in their talk and illustrate how these discourses interplayed and animated the meaning of participants’ Christian family identities. The …

Off The Rails: Cinematic Trains As Technological Controls Of The Natural World, Trinity Thompson

Honors Program: Senior Projects (Public)

Short train rail lines across the United States are seeing increased national funding to reduce toxic chemical spills caused by train derailments, the most notable of which happened in February 2023 in East Palestine, Ohio. A year prior, the film White Noise (2022) featured a similar toxic train derailment incident, taking place, too, in Eastern Ohio, and featuring actors from the town of East Palestine. In considering other films featuring trains, I identified a pattern of environmental conflict, leading me to question the relationship between trains and the natural environment as portrayed in popular cinema. To conduct my research, I …

Tame The Flame: Inflammation Is A Targetable Physiological Mechanism Underlying Poor Outcomes Of Heat Stress In Finishing Sheep, Pablo C. Grijalva

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This research focused on health management through the identification of suitable interventions in stressed livestock. For this investigation, we conducted two experiments. First, we determined whether administration of dexamethasone or fish oil to heat-stressed livestock to reduce systemic inflammation would mitigate metabolic deficits, enhance nutrient utilization, and improve health. We found that these interventions helped to reduce circulating inflammatory cytokines and improve respiration, muscle glucose metabolism, lipid homeostasis, cardiovascular function, and organ pathology in lambs exposed to 30 days of heat stress. In our second experiment, we performed transcriptomic analyses to identify the underlying mechanisms affecting young offspring following prenatal …

Characterization And Evaluation Of Bioactivity Of Dry Edible Bean And Bean Derived Small Peptides, Bikram Prasad Upadhyaya

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Obesity, diabetes, and their metabolic complications continue to be one of the major global public health concerns with a few effective treatment options. Although regular exercise and a well-balanced diet that includes calories from pulses can be recommended, much remains to be elucidated about the health effects of the various bioactive compounds including peptides and polyphenols present in pulses. Most of the previous studies have focused on individual dietary compounds and only a few investigated the synergistic effect of active compounds in modulating human health. Hence this dissertation aimed first, to provide a comparative evaluation of the overall therapeutic value …

Biomimetic Approach To Elucidate The Role Of Microenvironment In Regulating Cell Communications In Liver Diseases, Youra Moeun

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Liver is a complex organ but often underappreciated for its functions to remove toxins and fight infection in the body. Despite the ongoing prevalence of patients contracting chronic liver diseases, coupled with a global healthcare investment of approximately $70 billion, there is still no effective treatment for chronic liver diseases including alcoholic liver disease. The purpose of this dissertation was to understand the interactions of parenchymal and non-parenchymal liver cells in physiological and pathological like environments. This allowed us to recreate a healthy and the diseased microenvironments to observe the stages of the liver diseases development and apply appropriate treatments …

Gene Variants In Bola-Dmb, Decr1, Fasn And Srebf1 Associated With Conceptus Death On Day 16 Of Pregnancy In Holstein Cows [Abstract], Carolina L. Gonzalez-Berrios, Courtney Bowden, Pablo J. Pinedo, Jeanette V. Bishop, Hana Van Campen, Thomas R. R. Hansen, Milton G. Thomas

United States Department of Agriculture Wildlife Services: Staff Publications

Investigating the influences of single nucleotide polymorphisms (SNP) associated with conceptus death (CD) on day 16 of pregnancy could distinguish cows who have inferior value for fertility traits. We hypothesized that CD is associated with SNP genotypes that disrupt maternal recognition and lead to early pregnancy loss. RNA sequences from Holstein cow pregnancies (n = 15) with normal conceptuses (Norm) or CD were subjected to SNP discovery via Qiagen CLC Genomics Workbench. Selected SNP were based on previous fertility studies, differentially expressed genes within pregnancy development analyses and proximity to SNP associated with fertility traits in the Cattle QTL …

Students' Attitudes Towards Animals Influences Youth Development Constructs Based On Interactions With Different Animal Species Prior To College, Allison K. Pachunka

Department of Animal Science: Dissertations, Theses, and Student Research

Human-animal interactions (HAI) are commonplace in society and play a consequential role in a variety of situations such as companion animal ownership, agriculture, or youth programs such as 4-H or FFA. Interacting with animals has been shown to provide developmental benefits to children. Positive youth development (PYD), measured by the Five Cs Model, is a framework that focuses on fostering youth’s potential through positive activities which has been studied specifically in 4-H. However, this framework has not been applied to other organizations such as the National FFA Organization (FFA) or to other young adults with less formal interactions with animals. …

Discursive Struggles Reflected In The Communication Of Conservative Christian Parents And Their Adult Children With Differing Religious Beliefs And Values, Braedon G. Worman

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Nearly half of American adults no longer believe in their childhood faiths (Pew Research Center, 2015). The steady decline of Christianity could have considerable impacts on family life (Pew Research Center, 2022). From a postmodern critical perspective and guided by Relational Dialectics Theory 2.0, the researcher sought to discern how conservative Christian parents and their adult children with differing religious beliefs and values communicated when they discussed these differences, as well as to identify the discourses that informed and were reflected in their talk and illustrate how these discourses interplayed and animated the meaning of participants’ Christian family identities. The …

Preparing Students For Careers In Agriculture: An Analysis Of Secondary And Postsecondary Approaches To Work-Based Learning In The United States And Rwanda, Matthew Sterling Kreifels

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation investigates work-based learning as a method to prepare students for agricultural careers through three interconnected studies. The first two studies investigate secondary education programs in the United States, while the third study explores postsecondary education in Rwanda. In Study 1, a philosophical research approach explores how the new Supervised Agricultural Experience (SAE) approach, SAE for All, aligns five Foundational SAE components with learning theories and supports Career and Technical Education priorities. Study 2 utilizes a transcendental phenomenological approach to examine teacher perceptions of SAE for All's effectiveness for student career development. Study 3, a case study, analyzes how …

An Analysis Of Plant Response To Herbivory, Lawrence Gustavo Seminario-Romero

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Herbivory is often believed to always negatively affect the growth of plants, but there are plant species that can actually benefit from being consumed by herbivores. These plants have a phenotypic trait known as “compensatory growth.” A plant that exhibits compensatory growth is able to increase its intrinsic growth rate as a response to taking damage caused by herbivory, and as a result, produce more biomass. Very few mathematical models used to describe plant-herbivore interactions have accounted for plant compensatory growth. Moreover, these models tend to assume that herbivores follow a Holling type II functional response, which may not necessarily …

Skin Lipids Alone Enable Conspecific Tracking In An Invasive Reptile, The Argentine Black And White Tegu Lizard (Salvator Merianae), M. Rockwell Parker, Eric A. Tillman, Lauren A. Nazarian, Megan L. Barlowe, Julianna M. Lincoln, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Locating potential mates in non-native habitats is one of the most important challenges faced by invasive vertebrate species. The Argentine black and white tegu lizard (Salvator merianae) is a major invasive reptile species in the contiguous United States and is rapidly expanding its range across Florida and the Southeast, in part due to inadequate management strategies and tools. Because a wide array of reptiles, especially squamates (snakes and lizards), have been well-studied for their reliance on chemical cues to locate conspecifics, our project aimed to isolate chemical cues from tegus and assess the ability of adult males and …

Changes In Lipid Profiles Of Epileptic Mouse Model, Alicia Johnson, Ryan A. Grove, Deepak Madhavan, Cory Honsinger Thomas Boone, Camila Pereira Braga, Hannah Kyllo, Kaeli Samson, Timothy Simeone, Tomáš Helikar, Corrine K. Hanson, Jiri Adamec

Department of Biochemistry: Faculty Publications

Introduction—Approximately 1% of the world’s population is impacted by epilepsy, a chronic neurological disorder characterized by seizures. One-third of epileptic patients are resistant to AEDs, or have medically refractory epilepsy (MRE). One non-invasive treatment that exists for MRE includes the ketogenic diet, a high-fat, low-carbohydrate diet. Despite the KD’s success in seizure attenuation, it has a few risks and its mechanisms remain poorly understood. The KD has been shown to improve metabolism and mitochondrial function in epileptic phenotypes. Potassium channels have implications in epileptic conditions as they have dual roles as metabolic sensors and control neuronal excitation.

Objectives—The …

Pleistocene Bats (Late Irvingtonian And Late Rancholabrean) From Nuckolls And Sherman Counties, Nebraska, Nicholas J. Czaplewski, Richard George Corner

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

We document rare finds of fossil bats from two localities representing the Pleistocene epoch in southern and central Nebraska, Albert Ahrens locality (No-104, late Irvingtonian age, Middle Pleistocene), Nuckolls County, and Litchfield (Sm-102, late Rancholabrean age, latest Pleistocene), Sherman County. The Albert Ahrens local fauna with strong boreal influence has produced two bats, Lasiurus cf. borealis and Cf. Myotis sp. The Litchfield local fauna, also showing a strong boreal influence, has yielded two bats, Eptesicus fuscus and Cf. Myotis, among a diverse Pleistocene fauna of small vertebrates and a pollen record indicating a boreal mixed conifer and deciduous woodland, …

Simian Immunodeficiency Virus-Infected Rhesus Macaques With Aids Co-Develop Cardiovascular Pathology And Encephalitis, Kevin S. White, Joshua A. Walker, John Wang, Patrick Autissier, Andrew D. Miller, Nadia N. Abuelezan, Rachel Burrack, Qingsheng Li, Woong-Ki Kim, Kenneth C. Williams

Nebraska Center for Virology: Faculty Publications

Despite effective antiretroviral therapy, HIV co-morbidities remain where central nervous system (CNS) neurocognitive disorders and cardiovascular disease (CVD)-pathology that are linked with myeloid activation are most prevalent. Comorbidities such as neurocogntive dysfunction and cardiovascular disease (CVD) remain prevalent among people living with HIV. We sought to investigate if cardiac pathology (inflammation, fibrosis, cardiomyocyte damage) and CNS pathology (encephalitis) develop together during simian immunodeficiency virus (SIV) infection and if their co-development is linked with monocyte/ macrophage activation. We used a cohort of SIV-infected rhesus macaques with rapid AIDS and demonstrated that SIV encephalitis (SIVE) and CVD pathology occur together more frequently …