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New Interdisciplinary Research At 48pa551: Land Tenure And Subsistence Strategies Among Middle Holocene Rocky Mountain Hunter-Gatherers, Ethan Patrick Ryan

Graduate Student Theses, Dissertations, & Professional Papers

This dissertation studies land tenure and resource procurement strategies among Middle Holocene hunter-gatherers in the Greater Yellowstone Ecosystem (GYE). The research provides a better understanding of a stratified archaeological site on the eastern flank of the Yellowstone Plateau, 48PA551, a crucial location for winter resource procurement. Geophysical survey, collection’s research, obsidian sourcing, and stable isotope analysis are all used to test theoretical models of social conditions and hunting strategies during the harsh winters of the mountainous environment of northwest Wyoming. Site 48PA551 has already proven to contain evidence for a unique Middle Archaic adaptive strategy, being one of the only …

Subfamily Clustering Using Label Uncertainty (For Transposable Element Families), Audrey M. Shingleton

Graduate Student Theses, Dissertations, & Professional Papers

Biological sequence annotation is typically performed by aligning a sequence to a database of known sequence elements. For transposable elements, these known sequences represent subfamily consensus sequences. When many of the subfamily models in the database are highly similar to each other, a sequence belonging to one subfamily can easily be mistaken as belonging to another, causing non-reproducible subfamily annotation. Because annotation with subfamilies is expected to give some amount of insight into a sequence’s evolutionary history, it is important that such annotation be reproducible. Here, we present our software tool, SCULU, which builds upon our previously-described methods for computing …

Combined Effects Of Temperature And Heavy Metals On The Performance Of The Giant Salmonfly., James Frakes, Amanda Andreas, Benjamin P. Colman, Aurthur Woods

Graduate Student Theses, Dissertations, & Professional Papers

In many freshwater ecosystems, communities of aquatic insects are facing the combined stresses of warmer waters due to climate change and increased exposure to heavy metal toxicants. Although each stressor may threaten aquatic insects independently, they also likely interact in important ways to affect insect physiology and performance. Here we investigate this potential interaction using two populations of aquatic nymphs of the giant salmonfly, Pteronarcys californica, collected from adjacent rivers in Montana: naïve individuals from Rock Creek, a relatively pristine stream, and individuals from the Upper Clark Fork River, which has a history of heavy metal pollution and higher …

Facing The Fire - Challenges And Triumphs Of Western Firefighters In A Changing Climate, Mak Sisson

Graduate Student Theses, Dissertations, & Professional Papers

The American West sits at a dangerous position in the age of climate change. Wildfires burn hotter, longer, and cover more area than ever measured. Decreased snowpack, early spring run-off, a glut of built-up timber in parks and public lands, and longer droughts all build upon one another, and it just takes one spark to start a raging wildfire that devastates a community. As such, a firefighting force is needed to deal with this changing landscape.

However, that force is dwindling, especially at the federal level. A reported 20% of wildland firefighting positions are vacant, and the Bureau of Land …

Keeping What You Sow: Intellectual Property Rights For Plant Breeders And Seed Growers, Paulina B. Jenney

Graduate Student Theses, Dissertations, & Professional Papers

Over the last 150 years, the food system in the present-day United States has undergone a transformational restructuring, from a diversified, decentralized, network of farmers and seed growers, to one in which the majority of crop production is controlled by a few industrial corporations. The consolidation of power has been under-girded by the application of intellectual property rights (IPR)—especially utility patents—to plant varieties and genetic traits, which are leveraged to exclude small-scale seed growers from accessing quality germplasm. Patents and restrictive licensing agreements recapitulate colonial structures by appropriating common and traditionally community-held resources for profit, and by creating reliance on …

Where Should Conservation Draw The Line And When Should It Push Forward, Jessica L. Mejia

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Evaluating The Management And Consequences Of Hybridization Between Nonnative Rainbow Trout And Native Westslope Cutthroat Trout, Anthony James Dangora

Graduate Student Theses, Dissertations, & Professional Papers

The introduction of nonnative fish is a major driver in the decline of native fish species. Nonnative rainbow trout (Oncorhynchus mykiss; RBT) introduced into the native range of westslope cutthroat trout (O. clarkii lewisi; WCT) have led to the introgressive hybridization between these two species. This widespread hybridization is a primary threat to the long-term persistence of WCT as it can cause population-level genomic extinction. Since there are no set management solutions for hybridization, there is a need to evaluate the different conservation approaches to ensure the persistence of WCT populations. Additionally, beyond propagule pressure, the array of drivers that …

Is Growing Food Wasting Water? Stories From A Surge Of Groundwater Regulations In California, Abigail Jean Lauten-Scrivner

Graduate Student Theses, Dissertations, & Professional Papers

This long-form feature story examines how the nascence of California’s new era of groundwater regulation is playing out for the farmers, families and agencies who must adapt to a sweeping new law against the backdrop of climate change.

California became the last state in the west to adopt a statewide management plan for the invaluable natural resource when it first embarked on a path toward groundwater sustainability in 2020. Known as the Sustainable Groundwater Management Act (SGMA), the 20-year plan requires California’s most depleted groundwater basins to become sustainable by 2040.

When SGMA matured in 2020, California’s next drought began …

The Contrasting Physiological Mechanisms Of Two Distinct Migratory Strategies, Joely Gabriel Desimone

Graduate Student Theses, Dissertations, & Professional Papers

Migration allows animals to use resources that vary in time and space. The predictability of this resource variation underlies different migratory strategies. Obligate migrants, with predictable movements, use resources that covary with the seasonal phenology of temperature or rainfall patterns. Facultative migrants, with unpredictable timing and destination of movements, rely on unpredictably variable resources. In this dissertation I explore the physiological changes birds undergo in preparation for obligate vs. facultative migratory flight. Hormones facilitate rapid organismal responses to environmental and internal information, making them an ideal physiological system to understand mechanisms controlling migratory behavior. I focus on corticosterone (CORT), a …

Tracing Migration And Social Stratification In Subadults At The Archaeological Site Of Convento: A Preliminary Strontium Isotopic Analysis, Holli Kaye Mcdonald

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Relationships Between Water, Sediment, Basal Resources, And Aquatic Insects In A Mine-Contaminated River On A Longitudinal And Habitat Patch Scale, Chelsea A. Wisotzkey

Graduate Student Theses, Dissertations, & Professional Papers

It is well established that aqueous exposure and dietary exposure both lead to the accumulation of metals and metalloids in aquatic organisms living in a contaminated environment, but the relative contribution of each remains unclear. To examine how a contamination gradient affects patterns of metal(loid)s among periphyton, BPOM (benthic particulate organic matter), water, and sediment, we collected samples from multiple sites across a 210km stretch of the mine-contaminated Upper Clark Fork River, Montana. To investigate metal(loid) accumulation patterns among basal resources and aquatic insects, we collected samples of periphyton, BPOM, and larval insects from discrete habitat types at a single …

Spermatogenesis Molecular Evolution In Murine Rodents, Emily Emiko Konishi Kopania

Graduate Student Theses, Dissertations, & Professional Papers

Reproductive traits are fascinating from an evolutionary perspective because they are necessary for individuals to produce offspring and increase their evolutionary fitness. Given the essentiality of reproduction to fitness, genes involved in reproduction may be expected to be highly conserved. However, some genes involved in reproduction evolve very rapidly, including many spermatogenesis genes. This rapid evolution may result from intense sexual selection acting on reproductive traits, particularly in species where females mate multiply thus creating the potential for sperm competition. In addition to sexual selection, other evolutionary forces may shape rapid spermatogenesis evolution, including genomic conflict and relaxed pleiotropic constraint …

Spatial Behavior, Foraging, And Population Dynamics Of A Subarctic Ungulate, Elizabeth (Libby) Parr Williamson Ehlers

Graduate Student Theses, Dissertations, & Professional Papers

The concept of density-dependence is foundational to our understanding of the ecology and management of large herbivores. As the abundance of large herbivores increases, competition for high-quality resources increases and animals select for lower-quality foods potentially resulting in reduced vital rates that cause declines in population growth. What remains unclear is how foraging at finer scales, like the ‘bite’, translates to demography. This is difficult because it’s challenging to scale bite-level foraging to population-levels. I test the fine-scale foraging mechanisms of density dependence in a large subarctic herbivore, Barrenground caribou (Rangifer tarandus granti). The nutritional landscape for arctic herbivores is …

The Syndemic Landscape: A New Paradigm For Montana Suicide Prevention Grounded In Agricultural Renewal, Emory Chandler Padgett

Graduate Student Theses, Dissertations, & Professional Papers

Montana has had one of the highest suicide rates in the nation for half a century, and since 2000, it has risen almost 50%. Despite suicide’s alarming persistence in the state, there has been minimal academic study of suicide or mental health specifically in Montana, so this thesis attempts to answer a few questions: Why does Montana have such a high suicide rate? Is there something culturally, historically, or socially unique about Montana that contributes to suicide? Are current prevention efforts helpful, harmful, or lacking? Could a consideration of culture and land benefit an understanding of suicide in Montana? What …

Invasive Earthworms In The Crown Of The Continent System And Implications For Land Management, Meghan Elizabeth Scott

Graduate Student Theses, Dissertations, & Professional Papers

The United States contains invasive earthworms originating from Europe and Asia; the majority are European lumbricids. Direct introduction occurs primarily through human activity and, once established, earthworm populations are difficult to address. When exotic earthworms engage in bioturbation, they negatively alter subterranean food webs and nutrient cycling by disrupting soil layering systems. The most prominent form of physical alteration is the change and removal of the topmost organic layer. This disruption is associated with altered nitrogen and carbon cycling, as well as altered forest floor plant communities.

The Crown of the Continent ecosystem is located in southwestern Alberta, southeastern British …

Moccasin Economics: Entangled Museum Stories Of Niitsitapi Women, Labor, And Footwear, Michaela Ann Shifley

Graduate Student Theses, Dissertations, & Professional Papers

This dissertation emphasizes how anthropologists can use museum collections as anthropological data banks (Sturtevant 1973) to uncover the unwritten histories of objects, people, and cultures. I show how museum collections are repositories for the untold stories of Native women’s economic histories and how objects embody women’s critical contributions to the economic, spiritual, and cultural survival of their communities throughout time. To reveal the complex, hidden labor processes involved in historical and contemporary moccasin-making, I draw on interviews with contemporary Niitsitapi moccasin-makers, as well as object-based analyses of 109 pairs of moccasins from five museum collections and numerous archival documents and …

A Non-Deterministic Deep Learning Based Surrogate For Ice Sheet Modeling, Hannah Jordan

Graduate Student Theses, Dissertations, & Professional Papers

Surrogate modeling is a new and expanding field in the world of deep learning, providing a computationally inexpensive way to approximate results from computationally demanding high-fidelity simulations. Ice sheet modeling is one of these computationally expensive models, the model used in this study currently requires between 10 and 20 minutes to complete one simulation. While this process is adequate for certain applications, the ability to use sampling approaches to perform statistical inference becomes infeasible. This issue can be overcome by using a surrogate model to approximate the ice sheet model, bringing the time to produce output down to a tenth …

Combinatorial Administration Of Synthetic Tlr4 Agonist Ini-2002 And Novel Mincle Agonist Um-1098 Delivered Via A-Snps Results In Synergistic Il-1Β Production In Human Primary Cells And Enhances Th1 And Th17 Responses In Vivo, Grace D. Jones, Asia Marie Stephanie Riel, Alexander Riffey, Cassandra Buhl

Graduate Student Theses, Dissertations, & Professional Papers

Tuberculosis (TB) kills more people each year than any infectious disease worldwide with recent exception of SARS-CoV-2. Though the Bacille Calmette Guerin (BCG) vaccine confers protection against severe extrapulmonary forms of TB, there is no licensed vaccine for the prevention of pulmonary tuberculosis. The strongest correlate of protection against pulmonary tuberculosis is Th1/Th17 biased cell mediated immunity. Several candidates for TB vaccine adjuvants have shown Th1/Th17 polarizing capacity in clinical trials including Mincle agonist trehalose dibehenate (TDB) and TLR4 agonist monophosphoryl lipid A (MPL). Furthermore, combinatorial administration of MPL and TDB formulated in dimethyldioctadecylammonium (DDA) liposomes has been previously reported …

Rethinking Rare: Novel Approaches To Rare Species Monitoring And Conservation, Jessie Deanne Golding

Graduate Student Theses, Dissertations, & Professional Papers

Conservation of rare species is widely valued and important for ecosystems. Unfortunately, many of the approaches to conserve rare species have been developed with common species (e.g., harvested species) which have larger populations and targeted objectives. Conservation of rare species is difficult in part because of problems created by scarcity and low information. With low information, learning leads to new questions and the utility of information in decisions can quickly become obsolete. Therefore, monitoring strategies that can adapt as well as provide information tailored to relevant decisions are needed. To address rare species monitoring, I developed a long-term monitoring approach …

The Evolution Of Morphological Diversity And Sexual Dimorphism In Stick And Leaf Insects, Romain Pascal Gilbert Eric Boisseau

Graduate Student Theses, Dissertations, & Professional Papers

How has the diversity of life forms come to be? This question is at the core of evolutionary biology and can be addressed at different scales: by studying the processes that drive modifications within populations of organisms generation after generation (microevolution), or by investigating patterns of changes on the tree of life over long periods of time (macroevolution). Understanding the ultimate drivers of morphological diversity eventually entails connecting microevolutionary processes with macroevolutionary patterns. My dissertation investigates the diversification of body and egg form and its drivers in a relatively small but particularly diverse insect order: the stick and leaf insects …

The Effects Humidity & Temperature Has On Dna Contamination During Storage, Samantha L. Ramey

Graduate Student Theses, Dissertations, & Professional Papers

Studies have been conducted on DNA cross-contamination throughout the criminal investigation process in order to evaluation the possibility that DNA from one sample could potentially influence the outcome of another. However, no published studies have examined the potential for contamination during the storage of genetic evidence after samples have been taken from crime scenes or suspects. This study is a continuation of a preliminary project, which examined storage drying time in relation to cross-contamination. The current study tested temperature and humidity for the potential of cross-contamination during storage prior to extraction. Prior to storage, 50 μl of male saliva was …

Grizzly Bear Attractant Policies In Montana: How Regulations, Recommendations, And Resources Differ Among And Between Recovery Areas, Kenneth J. Edmo

Graduate Student Theses, Dissertations, & Professional Papers

Edmo, Kenneth, M.S, Autumn 2022 Resource Conservation

Grizzly bear attractant policies in Montana: how regulations, recommendations, and resources differ among and between recovery areas

Chairperson or Co-Chairperson: Alexander L. Metcalf & Chad J. Bishop

Abstract

Grizzly bears (Ursus arctos horribilis) are a keystone species and play an important role in their environment. After near extinction, the U.S. Fish and Wildlife Service implemented a recovery plan of six recovery zones to increase grizzly populations. Although these efforts allowed grizzly bear numbers to increase, so too have human-grizzly bear conflicts, significantly challenging agencies in charge of bear management. A concern …

Understanding The Underidentification Of Autism In Native American Students, Emily A. Brooke

Graduate Student Theses, Dissertations, & Professional Papers

School psychologists are called upon to promote nondiscriminatory practices that ensure equity and fairness for all youth, including racially minoritized students. Despite being overrepresented in almost every other disability category of special education, Native American students nationwide are underrepresented within the category of autism. The current study focuses on factors within educators that might lead to the underidentification of Native American students with autism. In alignment with the cultural humility model, and the tripartite model of multicultural competence embedded within it, the current study explored 36 educators’ attitudes, knowledge, and skills as they relate to the culturally responsive assessment of …

Characterizing Riverine Carbon: A Spatiotemporal Study Of Organic And Inorganic Carbon Variability And Evaluation Of Methods For Quantifying Pco2, Fischer Larson Young

Graduate Student Theses, Dissertations, & Professional Papers

Carbon is a critical component of aquatic ecosystems. For inland waters, carbon is a basal resource for biological communities and is a key component of trophic transfer through ingestion and respiration processes. Additionally, carbon acts as a key biogeochemical tracer that can track chemical, physical, and biological processes from the terrestrial landscape to inland waters to the ocean. Riverine carbon, however, can be challenging to characterize and interpret due to the complexity of its sources and sinks along a riverine continuum. Included in this work are the biogeochemically relevant forms of carbon such as dissolved inorganic carbon (DIC), the partial …

Toward A Dialectical Account Of Nature, Georgia Rae Grimm

Graduate Student Theses, Dissertations, & Professional Papers

The protection of nature has been a central aim of environmentalism for well over a century. However, the concept of nature has been subjected to abundant critiques in recent literature, threatening the conceptual tenability of this goal. In this paper, I discuss why I find the concept of nature too valuable to dismiss and offer an account of nature that I believe remedies existent critiques. In Chapter 1, I recount arguments for the protection of nature and illustrate their dualistic underpinnings. In Chapter 2, I discuss issues with dualistic accounts of nature and demonstrate why Steven Vogel’s monistic alternative is …

Applying Resource Selection Probability Function (Rspf) To Understand Floral Resource Use By A Common Bumble Bee, Bombus Vancouverensis, Rebekah Brassfield

Graduate Student Theses, Dissertations, & Professional Papers

Bumble bees (Hymenoptera: Bombus) are important pollinators in temperate ecosystems worldwide with seasonal caste variations involving queens, workers, and drones. Like all organisms, they must meet their nutrient requirements for successful growth and development by using several floral resources over time. When resource use is in greater proportion to its abundance on a landscape, that use is considered selective. To examine resource use within the context of abundance, a resource selection probability function (RSPF) was applied to examine floral resource use through a flowering season by a common bumble bee, Bombus vancouverensis. The RSPF framework was used to examine 1) …

Using Bone Biology To Enhance Forensic And Paleoanthropological Dna Analysis, Keith M. Biddle

Graduate Student Theses, Dissertations, & Professional Papers

We know that the optimal site for DNA extraction from human skeletal remains lies primarily in the petrous portion of the crania, and secondarily in the dental pulp, but we do not know why. As for the optimal location in the post-crania, targeted extraction sites are based on experience or inference, not empirical data. So, where to sample for DNA when only post-cranial elements are available? There are many instances where the petrous and/or teeth are not present or cannot be sampled. The three main goals of the project are 1- develop our foundational knowledge of the underlying cellular and …

Seeding Resilience: An Examination Of The Impacts Of A Seed Saving Network In Western Montana, Christina Leas

Graduate Student Theses, Dissertations, & Professional Papers

Seed saving, a worldwide practice as old as agriculture, continues even in the context of an increasingly industrialized and globalized agricultural system. While some scholarship has focused on informal seed saving practices that continue to thrive in the global South, few studies have examined the dynamics of these practices in the global North, particularly in the American West. Informal seed saving systems have implications for the resilience of agroecosystems. The concept of resilience has become an important framework for conceptualizing agroecosystems as social-ecological systems, both in scholarship and in policy. However, operationalizing the concept of resilience, particularly in agroecology research, …

Structural Mechanism Of Nuclear Receptor Biased Agonism, Michelle Deanna Nemetchek

Graduate Student Theses, Dissertations, & Professional Papers

Nuclear receptors are a class of ligand-activated transcription factors that affect gene expression. Pharmaceutical drugs that target nuclear receptors make up (13%) of all therapeutics prescribed in the United States. Unlike signaling pathways, nuclear receptors have a wide variety of binding partners that can bind upon activation of a nuclear receptor to upregulate gene transcription. These binding partner proteins, called “coregulators”, are diverse in function. However, the determinants of their binding to nuclear receptors, and how binding to certain coregulators over others occurs, is not known.

In this work, we examine how ligands create a preference for nuclear receptors to …

Development Of The Novel Isoxazolo[3,4-D]Pyridazinone Scaffold, Which Positively Modulates The Metabotropic Glutamate Receptor Subtypes 2 And 4, Christina Ann Gates

Graduate Student Theses, Dissertations, & Professional Papers

The seven transmembrane (7TM) superfamily, also known as G-protein coupled receptors (GPCR), are one of the largest superfamilies in the human genome. With approximately 30% of marketed drugs targeting the GPCRs, these proteins are among the most successful as therapeutic targets. Within the GPCR receptor family there is a subgroup called the metabotropic glutamate receptors (mGluR). Compounds that target mGluRs are important for the treatment of a variety of central nervous system (CNS) disorders, as well as cancer. The mGluR2 subtype is a target for treatment of anxiety and schizophrenia. Activation of mGluR4 helps to ease the symptoms of Parkinson’s …