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Articles 451 - 480 of 594
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A Comparison Study Of Subadult Long Bone Growth Between An Early Archaic Population (Windover) And A Late Archaic Population (Indian Knoll), Sunday A. Walker
A Comparison Study Of Subadult Long Bone Growth Between An Early Archaic Population (Windover) And A Late Archaic Population (Indian Knoll), Sunday A. Walker
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
It Depends On Us : Building U.S.-Russian Citizen Partnerships For Environmental Protection, Olga Maiboroda
It Depends On Us : Building U.S.-Russian Citizen Partnerships For Environmental Protection, Olga Maiboroda
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Origin And Spread Of Syphilis, Jun Hong
Origin And Spread Of Syphilis, Jun Hong
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Health Risk Behavior Among The Greek Population At The University Of Montana, Dean Patrick Mcgovern
Health Risk Behavior Among The Greek Population At The University Of Montana, Dean Patrick Mcgovern
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Demographic View Of Northern Cheyenne Women In 1900, Reno L. Charette-Loparco
Demographic View Of Northern Cheyenne Women In 1900, Reno L. Charette-Loparco
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Montana Kaimin, October 9, 1996, Associated Students Of The University Of Montana
Montana Kaimin, October 9, 1996, Associated Students Of The University Of Montana
Montana Kaimin, 1898-present
Student newspaper of the University of Montana, Missoula.
Directed Insertion Of A Selectable Marker Into A Circular Plasmid Of Borrelia Burgdorferi, Patricia Rosa, D. Scott Samuels, Daniel Hogan, Brian Stevenson, Sherwood Casjens, Kit Tilly
Directed Insertion Of A Selectable Marker Into A Circular Plasmid Of Borrelia Burgdorferi, Patricia Rosa, D. Scott Samuels, Daniel Hogan, Brian Stevenson, Sherwood Casjens, Kit Tilly
Biological Sciences Faculty Publications
Studies of the biology of Borrelia burgdorferi and the pathogenesis of Lyme disease are severely limited by the current lack of genetic tools. As an initial step toward facile genetic manipulation of this pathogenic spirochete, we have investigated gene inactivation by allelic exchange using a mutated borrelial gyrB gene that confers resistance to the antibiotic coumermycin A(1) as a selectable marker. We have transformed B. burgdorferi by electroporation with a linear fragment of DNA in which this selectable marker was flanked by sequences from a native borrelial 26-kb circular plasmid. We have identified coumermycin A(1)-resistant transformants in which gyrB had …
Montanan, Fall 1996, University Of Montana--Missoula
Montanan, Fall 1996, University Of Montana--Missoula
Montanan Magazine, 1969-2024
This is the magazine of the University of Montana with news about the University for UM alumni as well as current faculty, students, staff, and administrators. This is volume 14, number 1.
Six Distinguished Um Alumni Receive Awards At Homecoming, University Of Montana--Missoula. Office Of University Relations
Six Distinguished Um Alumni Receive Awards At Homecoming, University Of Montana--Missoula. Office Of University Relations
University of Montana News Releases, 1928, 1956-present
No abstract provided.
Analysis Of Linear Plasmid Dimers In Borrelia Burgdorferi Sensu Lato Isolates: Implications Concerning The Potential Mechanism Of Linear Plasmid Replication, Richard T. Marconi, Sherwood Casjens, Ulrike G. Munderloh, D. Scott Samuels
Analysis Of Linear Plasmid Dimers In Borrelia Burgdorferi Sensu Lato Isolates: Implications Concerning The Potential Mechanism Of Linear Plasmid Replication, Richard T. Marconi, Sherwood Casjens, Ulrike G. Munderloh, D. Scott Samuels
Biological Sciences Faculty Publications
The Borrelia genome is composed of a linear chromosome and a number of variable circular and linear plasmids. Atypically large linear plasmids of 92 to 105 kb have been identified in several Borrelia burgdorferi sensu lato isolates and characterized. These plasmids carry the p27 and ospAB genes, which in other isolates reside on a 50-kb plasmid. Here we demonstrate that these plasmids are dimers of the 50-kb ospAB plasmid (pAB50). The 94-kb plasmid from isolate VS116, pVS94, was an exception and did not hybridize with any plasmid gene probes. When this plasmid was used as a probe, homologous sequences in …
Sexually Transmitted Disease Expert Speaks At Um, University Of Montana--Missoula. Office Of University Relations
Sexually Transmitted Disease Expert Speaks At Um, University Of Montana--Missoula. Office Of University Relations
University of Montana News Releases, 1928, 1956-present
No abstract provided.
Main Hall To Main Street, February 1996, University Of Montana--Missoula. Office Of University Relations
Main Hall To Main Street, February 1996, University Of Montana--Missoula. Office Of University Relations
Main Hall to Main Street, 2005-2010
This is volume 2, number 2 of Main Hall to Main Street.
Montana Kaimin, January 30, 1996, Associated Students Of The University Of Montana
Montana Kaimin, January 30, 1996, Associated Students Of The University Of Montana
Montana Kaimin, 1898-present
Student newspaper of the University of Montana, Missoula.
Pmb10 Pollution And The Consumption Of Physician Services In Missoula County Montana, Michelle J. Gall
Pmb10 Pollution And The Consumption Of Physician Services In Missoula County Montana, Michelle J. Gall
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Selection And Characterization Of 2',3'-Dideoxy-2',3'-Didehydrothymidine-Resistant Mutants Of Feline Immunodeficiency Virus, Ya Qi Zhu
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Host Restriction Phenotypes Of Salmonella Typhi And Salmonella Gallinarum, Lisa Pascopella, Bärbel Raupach, Nafisa Ghori, Denise Monack, Stanley Falkow, P. L. C. Small
Host Restriction Phenotypes Of Salmonella Typhi And Salmonella Gallinarum, Lisa Pascopella, Bärbel Raupach, Nafisa Ghori, Denise Monack, Stanley Falkow, P. L. C. Small
Public and Community Health Sciences Faculty Publications
Salmonella typhi and Salmonella gallinarum phenotypes correlated with mouse host restriction have been identified by using in vitro and in vivo systems. S. typhi is capable of entering the murine intestinal epithelium via M cells, as is Salmonella typhimurium, which causes systemic infection in the mouse. But, unlike S. typhimurium, S. typhi does not destroy the epithelium and is cleared from the Peyer’s patches soon after M-cell entry. S. gallinarum appears to be incapable of entering the murine Peyer’s patch epithelium. Our in vitro evidence suggests that S. gallinarum is taken up in murine phagocytic cells by a mechanism different …
Selection And Characterization Of A 2',3'-Dideoxycytidine-Resistant Mutant Of Feline Immunodeficiency Virus, Holly Kay Medlin
Selection And Characterization Of A 2',3'-Dideoxycytidine-Resistant Mutant Of Feline Immunodeficiency Virus, Holly Kay Medlin
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Genetic Transformation Of The Lyme Disease Agent Borrelia Burgdorferi With Coumarin-Resistant Gyrb., D. Scott Samuels, Kathleen E. Mach, Claude F. Garon
Genetic Transformation Of The Lyme Disease Agent Borrelia Burgdorferi With Coumarin-Resistant Gyrb., D. Scott Samuels, Kathleen E. Mach, Claude F. Garon
Biological Sciences Faculty Publications
No useful method to genetically manipulate Borrelia burgdorferi, the causative agent of Lyme disease, has been developed previously. We have used resistance to the coumarin antibiotic coumermycin A1, an inhibitor of DNA gyrase, as a genetic marker to monitor the transformation of B. burgdorferi by electroporation. Introduction of site-directed mutations into the gyrB gene demonstrated that transformation was successful, provided evidence that homologous recombination occurs on the chromosome, and established that mutations at Arg-133 of DNA gyrase B confer coumermycin A1 resistance in B. burgdorferi. The coumermycin A1-resistant gyrB marker and genetic transformation can now be applied toward dissecting the …
"Canadian-American Border Alliance" (2), Max S. Baucus
"Canadian-American Border Alliance" (2), Max S. Baucus
Max S. Baucus Speeches and Remarks
"Canadian-American Border Alliance" (2)
Analysis Of The Distribution And Molecular Heterogeneity Of The Ospd Gene Among The Lyme Disease Spirochetes: Evidence For Lateral Gene Exchange, Richard T. Marconi, D. Scott Samuels, Robert K. Landry, Claude F. Garon
Analysis Of The Distribution And Molecular Heterogeneity Of The Ospd Gene Among The Lyme Disease Spirochetes: Evidence For Lateral Gene Exchange, Richard T. Marconi, D. Scott Samuels, Robert K. Landry, Claude F. Garon
Biological Sciences Faculty Publications
Analysis of the ospD gene has revealed that this gene is not universal among Lyme disease spirochete isolates. The gene was found to be carried by 90, 50, and 24% of the Borrelia garinii, B. afzelii, and B. burgdorferi isolates tested. Size variability in the ospD-encoding plasmid was also observed. Sequence analysis has demonstrated the presence of various numbers of a 17-bp repeated sequence in the upstream control (promoter) region of the gene. In addition, a region within the coding sequence where various insertions, deletions, and direct repeats occur was identified. ospD gene sequences from 31 different isolates were determined …
Gyrb Mutations In Coumermycin A1-Resistant Borrelia Burgdorferi., D. Scott Samuels, Richard T. Marconi, Wai Mun Huang, Claude F. Garon
Gyrb Mutations In Coumermycin A1-Resistant Borrelia Burgdorferi., D. Scott Samuels, Richard T. Marconi, Wai Mun Huang, Claude F. Garon
Biological Sciences Faculty Publications
We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.
People In Education, April 5, 1994, University Of Montana--Missoula. Office Of University Relations
People In Education, April 5, 1994, University Of Montana--Missoula. Office Of University Relations
University of Montana News Releases, 1928, 1956-present
No abstract provided.
Identification Of A Protein In Several Borrelia Species Which Is Related To Ospc Of The Lyme Disease Spirochetes., Richard T. Marconi, D. Scott Samuels, Tom G. Schwan, Claude F. Garon
Identification Of A Protein In Several Borrelia Species Which Is Related To Ospc Of The Lyme Disease Spirochetes., Richard T. Marconi, D. Scott Samuels, Tom G. Schwan, Claude F. Garon
Biological Sciences Faculty Publications
Using oligonucleotide probes which have previously been shown to be specific for the ospC gene found in the Lyme disease spirochete species Borrelia burgdorferi, B. garinii, and group VS461, we detected an ospC homolog in other Borrelia species including B. coriaceae, B. hermsii, B. anserina, B. turicatae, and B. parkeri. In contrast to the Lyme disease spirochetes, which carry the ospC gene on a 26-kb circular plasmid, we mapped the gene in other Borrelia species to linear plasmids which varied in size among the isolates tested. Some isolates carry multiple copies of the gene residing on linear plasmids of different …
People In Education, February 25, 1993, University Of Montana--Missoula. Office Of University Relations
People In Education, February 25, 1993, University Of Montana--Missoula. Office Of University Relations
University of Montana News Releases, 1928, 1956-present
No abstract provided.
Transcriptional Analyses And Mapping Of The Ospc Gene In Lyme Disease Spirochetes, Richard T. Marconi, D. Scott Samuels, Claude F. Garon
Transcriptional Analyses And Mapping Of The Ospc Gene In Lyme Disease Spirochetes, Richard T. Marconi, D. Scott Samuels, Claude F. Garon
Biological Sciences Faculty Publications
In Lyme disease spirochetes, the ospC gene encodes a 22.7-kDa protein referred to as either the pC or the OspC protein. Using a variety of electrophoretic approaches followed by Southern blotting and probing with oligonucleotide probes, we mapped the ospC gene to a circular 26-kb plasmid. The ospC gene represents the first gene to be mapped to a circular plasmid in Lyme disease spirochetes. The occurrence of this gene in isolates belonging to each of the three Lyme disease-associated species, Borrelia burgdorferi, Borrelia garinii, and the VS461 group, was evaluated. The ospC gene was found to occur in all 21 …
Coumermycin A1 Inhibits Growth And Induces Relaxation Of Supercoiled Plasmids In Borrelia Burgdorferi, The Lyme Disease Agent, D. Scott Samuels, Claude F. Garon
Coumermycin A1 Inhibits Growth And Induces Relaxation Of Supercoiled Plasmids In Borrelia Burgdorferi, The Lyme Disease Agent, D. Scott Samuels, Claude F. Garon
Biological Sciences Faculty Publications
Coumermycin A1 is an inhibitor of DNA gyrase, an enzyme that catalyzes supercoiling of DNA and is required for bacterial DNA replication. We have investigated the activity of this coumarin antibiotic on Borrelia burgdorferi, a spirochete and the causative agent of Lyme disease. B. burgdorferi was more susceptible than many other eubacteria to coumermycin as well as novobiocin, another coumarin antibiotic; this contrasted with its relative resistance to the DNA gyrase inhibitors nalidixic acid, oxolinic acid, and ciprofloxacin. Coumermycin at 0.2 micrograms/ml inhibited the growth of B. burgdorferi B31 in BSK II medium. A 100-fold-lower concentration induced the relaxation of …
Developing A Comprehensive Substance Abuse Program On The Flathead Reservation, Anna Whiting Sorrell
Developing A Comprehensive Substance Abuse Program On The Flathead Reservation, Anna Whiting Sorrell
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Social Support: The Effect Of Self Complexity And The Perceived Ability To Reciprocate, Colleen Murphy-Southwick
Social Support: The Effect Of Self Complexity And The Perceived Ability To Reciprocate, Colleen Murphy-Southwick
Graduate Student Theses, Dissertations, & Professional Papers
No abstract provided.
Montana Kaimin, May 14, 1992, Associated Students Of The University Of Montana
Montana Kaimin, May 14, 1992, Associated Students Of The University Of Montana
Montana Kaimin, 1898-present
Student newspaper of the University of Montana, Missoula.