Digital Commons Network^™

Identification Of The Pan‑Allergen Tropomyosin From The Common Bed Bug (Cimex Lectularius), Johnalyn Gordon, Zachary C. Devries

UK CARES Faculty Publications

Tropomyosin is a muscle contraction protein documented across all animal life. Despite its ubiquity, its unique structure in invertebrates leads to allergic responses in humans that vertebrate tropomyosin does not. High degrees of homology can explain cross‑reactivity between tropomyosin derived from distantly‑related arthropod species and establishes tropomyosin as a panallergen. Given this cross‑ reactivity and that they are commonly found in high numbers indoors, research on the potential of the common bed bug (Cimex lectularius L.) to contribute tropomyosin to the indoor environment is needed. Therefore, we investigated tropomyosin homology between bed bugs and known tropomyosin allergens from other taxa, …

Online Instructional Tools For Motor Carriers, Brian Howell, Jennifer Walton, Paul Ross, Andrew Martin

Kentucky Transportation Center Research Report

Commercial motor carrier customers on Kentucky highways must register and pay taxes for licensing and credentialing requirements. The Kentucky Transportation Cabinet (KYTC) provides an online Motor Carrier Portal that offers information and instructions on meeting these requirements, but customers have increasingly resorted to calling into KYTC’s motor carrier help desk, thereby straining limited personnel resources. The Kentucky Transportation Center (KTC) coordinated with KYTC to develop a series of online instructional videos that can better assist customers with applying for and meeting credentialing requirements.

KTC researchers used a three-phase approach to determine the feasibility and requirements for instructional videos. First, they …

Impact Of The New Context Functional Classifications For Kytc, Arlen Sandlin, Jill Asher, Nikiforos Stamatiadis, Jeff Jasper, Rachel Catchings, Chris Van Dyke

Kentucky Transportation Center Research Report

State transportation agencies are adopting an expanded context classification system to inform project development and delivery. This system classifies roadways into one of five categories based on factors such as level of development, building densities and setbacks, multimodal user patterns and requirements, network permeability, and speed. Compared to functional classification, context classification better captures the types of mobility, travel patterns, and user mixes observed in specific contexts. The expanded context classification system is found in AASHTO’s A Policy on Geometric Design of Highways and Streets (7^th Edition). The forthcoming 8^th edition will deepen integration of context classification throughout …

Pl 43 Traffic Forecasting Report 2023, Mei Chen, Xu Zhang, Eugene Antwi

Kentucky Transportation Center Research Report

This document is the seventh edition of the Traffic Forecasting Report (TFR) for the state of Kentucky. TFR data are important for multiple engineering applications that involve analyzing temporal and spatial variability in traffic distributions and volumes. Most of the metrics discussed in the report were computed using data from 2019, 2021, and 2022 — excluded from consideration were 2020 data because they exhibited anomalous traffic patterns tied to shutdowns associated with the COVID-19 pandemic. Data summarized in this report include average daily traffic, annual average daily truck traffic, travel activity by vehicle type, weekday and weekend hourly volume percentages, …

Chinese Digital Platform Companies’ Expansion In The Belt And Road Countries, Yujia He

Patterson School of Diplomacy and International Commerce Faculty Publications

The emergence of digital platforms is shifting the digital economy toward a platform economy, and Chinese platform-based businesses like Alibaba, Tencent, and JD are increasingly expanding in the Global South. Alongside this, the Chinese government has been promoting digital economy collaboration with emerging markets through high-level engagement under the banner of the Belt and Road Initiative (BRI) and its digital economy component the Digital Silk Road (DSR). Despite significant market interest and policy attention, grounded empirical analysis of Chinese digital platforms’ expansion within Belt and Road Initiative countries is scarce. This study employs a mixed-methods approach, drawing on both quantitative …

The Janssen Effect And The Chini Ordinary Differential Equation, Adam Rogers, George Dyck, Jitendra Paliwal, Kurt Hildebrand, Michael D. Montross, Aaron P. Turner

Chemical and Materials Engineering Faculty Publications

The Janssen effect describes the increase in pressure with depth in granular materials. However, the original Janssen formulation treats the density as constant. In this work, we examine parametric density models that depend on both pressure and moisture content and allow for temperature dependence to be easily included. We focus on physically-motivated empirical models that are built on power-law terms. These power-law terms correspond to thermodynamic equations of state, such as the ideal gas law, and polytropic processes. When coupled with the Janssen ordinary differential equation (ODE), these power-law models have the form of a Chini ODE. We study the …

Adverse Childhood Experiences And Early Initiation Of Substance Use: A Survival Analysis., Amy L. Meadows, Justin C Strickland, S Maela Hyder, Rita C Basconi, Margaret E Stull, Frances P Wagner, Mai N Nguyen, Abner O. Rayapati, Craig R Rush

Psychiatry Faculty Publications

OBJECTIVE: Early adversity, such as adverse childhood experiences (ACEs), is a risk factor for the development of substance use disorder (SUD). ACEs are associated with earlier initiation of substance use. This study examined the relationship between ACEs and age of initiation of substance use using survival analysis. It is hypothesized that individuals with higher ACEs will have an earlier age of initiation.

METHOD: Participants were recruited from the University of Kentucky's Laboratory for Human Behavioral Pharmacology. Participants were 18 years or older, English speaking, and actively engaged in substance use. Participants were not in substance abuse treatment nor were they …

Protocol For Modeling And Simulating Lithiation-Induced Stress In Largely Deformed Spherical Nanoparticles Using Comsol, Yong Li, Yungpeng Guo, Yunhao Wu, Yin Rao, Kai Zhang, Dashun Liu, Fuqian Yang

Chemical and Materials Engineering Faculty Publications

Here, we present a finite element method-based scheme for solving coupled par- tial differential equations (PDEs) for the analysis of lithiation-induced stress in largely deformed spherical nanoparticles via the PDE module in COMSOL. We describe steps for software installation and setting PDEs, initial/boundary condi- tions, and mesh parameters. We then detail procedures for dividing the mesh and analyzing lithium trapping during electrochemical cycling. This protocol can also be extended to analyze a wide range of problems involving diffusion-induced stress. For complete details on the use and execution of this protocol, please refer to Li et al.

Strengthening The Deployment Of Uncrewed Aerial Systems (Uas) At Kytc, Rachel Catchings, Suzanne Smith, Gayle Marks, Candice Wallace, Christopher Van Dyke

Kentucky Transportation Center Research Report

The Kentucky Transportation Cabinet (KYTC) and transportation agencies around the United States increasingly depends on uncrewed aircraft systems (UAS — also commonly known as drones) to collect data and accelerate project development and delivery. Drones let transportation practitioners safely and efficiently perform many activities, including bridge and highway inspection, construction monitoring, surveying, emergency response, and measuring stockpiles. KYTC’s implementation of UAS has expanded rapidly over the past 10 years, however, the agency lacks well-coordinated procedures for connecting pilots with end data users, scheduling and conducting flights, developing and documenting best practices, and processing and storing data. Based on a review …

Best Practices For Recruitment And Retention Of Transportation Professionals, Chris Van Dyke, Bryan Gibson, Sarah Mccormack, Merl Hackbart, Doug Kreis

Kentucky Transportation Center Research Report

Many public sector agencies, including state departments of transportation (DOTs), have long found recruiting and retaining staff a daunting challenge. Lured by the promise of higher salaries in the private sector, many people are reluctant to sacrifice their long-term earnings potential to pursue public service. And with the quality of fringe benefits (e.g., retirement plans, health insurance) offered by public agencies having declined gradually, it is even more difficult to persuade prospective employees to accept a lower salary. Many studies over the past 15—20 years have looked at methods for solving the problem of recruitment and retention within the context …

Of Hearth And Home: Professional Wellness After Hours, Timothy J. Ainger, Meriem Bensalem-Owen, Joanna Fong-Isariyawongse, Matthew William Luedke

Neurology Faculty Publications

Burnout is a prevalent problem in the contemporary practice of medicine. Defined by the Agency for Healthcare Research and Quality as, “a long-term stress reaction marked by emotional exhaustion, depersonalization, and a lack of sense of personal accomplishment,” this multifactorial condition has significant implications for the clinicians who suffer it, their patients, and families. Neurologists suffer some of the highest rates of burnout. Burnout research on interventions often focus on the work environment. In this article, we will focus on burnout’s effects on home life and features of home life that can impact resiliency, specifically sleep hygiene.

Mnemonic Utilization In Stroke Education: Fast And Befast Adoption By Certified Comprehensive Stroke Centers, Christopher Hogge, Larry B. Goldstein, Sushanth R. Aroor

Neurology Faculty Publications

Introduction: Symptom recognition and timely access to treatment are critical components of acute stroke care systems. Two mnemonics widely used in public educational campaigns for recognizing stroke symptoms include FAST (Face-Arm-Speech-Time) and BEFAST (Balance-Eyes-Face-Arm Speech-Time). The FAST mnemonic can miss up to 14% of strokes. BEFAST includes common posterior circulation stroke symptoms and has been implemented by several Comprehensive Stroke Centers (CSCs).

Methods: We sought to analyze the pattern of public educational materials available on the websites of US CSCs. The Joint Commission (JC) quality check website compiles a list containing the names and locations of the country’s 217 JC-certified …

Equity Of Overdose Education And Naloxone Distribution Provided In The Kentucky Healing Communities Study, Douglas R. Oyler, Hannah K. Knudsen, Carrie B. Oser, Sharon L. Walsh, Monica Roberts, Shawn R. Nigam, Philip M. Westgate, Patricia R. Freeman

Biostatistics Faculty Publications

Background: Opioid overdoses differentially affect demographic groups. Strategies to reduce overdose deaths, specifically overdose education and naloxone distribution (OEND), are not consistently delivered equitably. Methods: The HEALing Communities Study^SM (HCS) is a cluster-randomized trial designed to implement evidence-based practices, including OEND, to reduce overdose deaths across communities. Individuals receiving OEND in eight Kentucky counties between January 2020 and June 2022 provided demographics and overdose history. Recipient characteristics were compared to opioid overdose decedent characteristics to evaluate whether OEND was equitably delivered to the target population. Recipient characteristics were also analyzed based on whether OEND was delivered in criminal justice, …

Older Adults Using Social Support To Improve Self-Care (Oasis), Brittany L. Smalls, Aaron Kruse-Diehr, Courtney L. Ortz, Key Douthitt, Christopher Mclouth, Rachel Shelton, Zoe Taylor, Edith Williams

Biostatistics Faculty Publications

Introduction The prevalence of type 2 diabetes (T2D) is 17% higher in rural dwellers compared to their urban counterparts, and it increases with age, with an estimated 25% of older adults (≥ 65 years) diagnosed. Appropriate self-care is necessary for optimal clinical outcomes. Overall, T2D self-care is consistently poor among the general population but is even worse in rural-dwellers and older adults. In rural Kentucky, up to 23% of adults in Appalachian communities have been diagnosed with T2D and, of those, 26.8% are older adults. To attain optimal clinical outcomes, social environmental factors, including social support, are vital when promoting …

Quantitative Foveal Structural Metrics As Predictors Of Visual Acuity In Human Albinism, Erica N. Woertz, Gelique D. Ayala, Niamh Wynne, Sergey Tarima, Serena Zacharias, Murray H. Brilliant, Taylor M. Dunn, Deborah Costakos, C. Gail Summers, Sasha Strul, Arlene V. Drack, Joseph Carroll

Biostatistics Faculty Publications

PURPOSE. To assess the degree to which quantitative foveal structural measurements account for variation in best-corrected visual acuity (BCVA) in human albinism. METHODS. BCVA was measured and spectral domain optical coherence tomography (SD-OCT) images were acquired for 74 individuals with albinism. Categorical foveal hypoplasia grades were assessed using the Leicester Grading System for Foveal Hypoplasia. Foveal anatomical specialization (foveal versus parafoveal value) was quantified for inner retinal layer (IRL) thickness, outer segment (OS) length, and outer nuclear layer (ONL) thickness. These metrics, participant sex, and age were used to build a multiple linear regression of BCVA. This combined linear model's …

Abdominal Aortic Aneurysms And Platelets: Infiltration, Inflammation, And Elastin Disintegration, Bowen Li, Hong S. Lu, Alan Daugherty

Saha Cardiovascular Research Center Faculty Publications

No abstract provided.

Local Strain Quantification Of A Porous Carbon Fiber Network Material, Robert N Quammen, Paul F. Rottmann

Chemical and Materials Engineering Faculty Publications

While porous materials’ wide range of attractive functional properties have led to their development for a variety of applications, their intrinsically stochastic microstructures prevent straightforward approaches to predicting their mechanical behavior. This is attributed to the mechanisms that govern the macroscale behavior of these materials operating on multiple microstructure-specific length scales spanning several orders of magnitude. The goal of this work was to experimentally observe these operative deformation mechanisms to better improve the development of mechanism-informed models that more accurately predict the behavior of these materials. In this study compression tests were conducted on a porous carbon fiber network material. …

Borrelia Burgdorferi Plza Is A Cyclic-Di-Gmp Dependent Dna And Rna Binding Protein, Nerina Jusufovic, Andrew Krusenstjerna, Christina R. Savage, Timothy C. Saylor, Catherine A. Brissette, Wolfram Zückert, Paula J. Schlax, Md A. Motaleb, Brian Stevenson

Entomology Faculty Publications

The PilZ domain-containing protein, PlzA, is the only known cyclic di- GMP binding protein encoded by all Lyme disease spirochetes. PlzA has been implicated in the regulation of many borrelial processes, but the effector mechanism of PlzA was not previously known. Here, we report that PlzA can bind DNA and RNA and that nucleic acid binding requires c-di-GMP, with the affinity of PlzA for nucleic acids increasing as concentrations of c-di-GMP were increased. A mutant PlzA that is incapable of bind- ing c-di-GMP did not bind to any tested nucleic acids. We also determined that PlzA interacts predominantly with the …

Spontaneous Alignment Of Myotubes Through Myogenic Progenitor Cell Migration, Lauren E. Mehanna, Adrianna R. Osborne, Charlotte A. Peterson, Brad J. Berron

Chemical and Materials Engineering Faculty Publications

In large-volume muscle injuries, widespread damage to muscle fibers and the surrounding connective tissue prevents myogenic progenitor cells (MPCs) from initiating repair. There is a clinical need to rapidly fabricate large muscle tissue constructs for integration at the site of large volume muscle injuries. Most strategies for myotube alignment require microfabricated structures or prolonged orientation times. We utilize the MPC’s natural propensity to close gaps across an injury site to guide alignment on collagen I. When MPCs are exposed to an open boundary free of cells, they migrate unidirectionally into the cell-free region and align perpendicular to the original boundary …

National Trends Of Vascular Risk Factor Control Among Stroke Survivors: From The National Health And Nutrition Examination Survey 2009 To 2020, Hend Mansoor, Daniel Manion, Kara Jo Swafford, Gregory A. Jicha, Daniela Claudia Moga

Neurology Faculty Publications

BACKGROUND: Contemporary data describing the national trends on vascular risk factor control among stroke survivors are limited.

METHODS AND RESULTS: This is a cross-sectional analysis of the National Health and Nutrition Examination Survey cycles 2009 to 2010 to 2017 to March 2020. Adults (≥18 years of age) with a self-reported diagnosis of stroke were identified. Age- adjusted trends in hypertension, diabetes, and hyperlipidemia control were examined. Sex and racial differences in vascular risk factor control were also investigated. Among 32 497 adult individuals who participated in the National Health and Nutrition Examination Survey, 1354 participants (4.2%) self-reported a prior diagnosis …

Financial-Legal Navigation Reduces Financial Toxicity Of Pediatric, Adolescent, And Young Adult Cancers, Jean Edward, Kimberly D. Northrip, Mary Kay Rayens, Andrea Welker, Rachel O'Farrel, Jennifer Knuf, Haafsah Fariduddin, Julia Costich, John A. D'Orazio

Markey Cancer Center Faculty Publications

Background: Pediatric, adolescent, and young adult patients with cancer and their caregivers are at high risk of financial toxicity, and few evidence-based oncology financial and legal navigation programs exist to address it. We tested the feasibility, acceptability, and preliminary effectiveness of Financial and Insurance Navigation Assistance, a novel interdisciplinary financial and legal naviga- tion intervention for pediatric, adolescent and young adult patients and their caregivers.

Methods: We used a single-arm feasibility and acceptability trial design in a pediatric hematology and oncology clinic and collected preintervention and postintervention surveys to assess changes in financial toxicity (3 domains: psychological response/ Comprehensive Score …

Staphylococcus Aureus Oleate Hydratase Produces Ligands That Activate Host Ppara, Christopher D. Radka, Matthew W. Frank, Tyler S. Simmons, Cydney N. Johnson, Jason W. Rosch, Charles O. Rock

Markey Cancer Center Faculty Publications

Commensal gut bacteria use oleate hydratase to release a spectrum of hydroxylated fatty acids using host-derived unsaturated fatty acids. These compounds are thought to attenuate the immune response, but the underlying signaling mechanism(s) remain to be established. The pathogen Staphylococcus aureus also expresses an oleate hydratase and 10- hydroxyoctadecanoic acid (h18:0) is the most abundant oleate hydratase metabolite found at Staphylococcal skin infection sites. Here, we show h18:0 stimulates the transcription of a set of lipid metabolism genes associated with the activation of peroxisome proliferator activated receptor (PPAR) in the RAW 264.7 macrophage cell line and mouse primary bone marrow-derived …

Germplasm Enhancement Of Annual Forage Legumes Suitable For Use In Cold Highland Environments, H K. Firincioglu, N Karagullu, S Unal, A M. Abd-El Moneim

IGC Proceedings (1977-2023)

The Central Highlands (CH) of Turkey are characterized by cold winters and dry summers. Under these extreme air temperatures, forage crops are still underexploited as a part of crop rotations. Half of the total vetch hectarage in Turkey is in the CH, and farmers still use the local cultivars that have good adaptability but low yield potential. Therefore, with the aim of identifying and improving the annual forage legume species for the cold and dry environment of the CH, research work was carried out during the 1993/94 and 1994/ 95 crop seasons. Initial results showed that Hungarian vetch (Vicia …

Use Of The Grazing Animal In Forage Breeding, J H. Bouton, C S. Hoveland, R N. Gates

IGC Proceedings (1977-2023)

The objective of these studies was to determine, in the context of a forage breeding program, the effect of cattle grazing on survival of alfalfa (Medicago sativa L.), red clover (Trifolium pratense L.), and tall fescue (Festuca arundinacea Schreb.). Different cultivars of each species were compared in grazed and ungrazed areas. Results consistently demonstrated that grazing reduced plant stands when compared to ungrazed conditions for each species tested. In alfalfa, however, cultivars developed for grazing tolerance showed much better grazing survival. Infection with the fungal endophyte, Acremonium coenophialum Morgan-Jones & Gams, likewise substantially increased grazing survival in …

Study Of Genotype X Environment Interaction In Alfalfa Forage Yield, S N. Acharya, G C. Kozub, H Najda, A Aasen

IGC Proceedings (1977-2023)

The response of alfalfa (Medicago sativa L.) forage yield to eight Alberta test sites was studied for the 1990 and 1991 production years. Cluster analysis was used to group locations and cultivars. Analyses of variances indicated genotype x environment (location) interaction for the first cut yield, the total yield and the difference between first and second cut yields. The Brooks, Bow Island (irrigation), Bow Island (dryland) and Provost locations always clustered together indicating that three of these four test sites may be eliminated without sacrificing reliability.

Selection For Self-Fertility And Self-Fertility In Alfalfa As A Tool For Breeding Strategy Assessment, D Rosellini, F Veronesi, F Lorenzetti

IGC Proceedings (1977-2023)

Cultivar development in alfalfa relies on heterosis, that has been mainly exploited by intercrossing selected clones from diverse gemplasm sources; an alternative way was proposed, based on developing double cross cultivars obtained by crossing clones selected for self-sterility, propagated by somatic seed. A different breeding strategy was recently emphasized based on population improvement through inbreeding and recurrent selection; in this case, self-sterility would be selected against. If self-sterility is determined by genetic load, the second strategy should be preferred, and selecting for self-fertility may bring about positive correlated responses for forage yield. Divergent selection for self-fertility was applied in a …

Morphological And Rapd Variations And Regenerants Derived From Cell Suspension Culture Of Pangolagrass, C S. Chen, S M. Wang, Y K. Cheng

IGC Proceedings (1977-2023)

Pangolagrass cv. A254, a sterile strain, is one of Taiwan's major forage sources but recently has faced serious disease problems. Tissue culture was expected to generate variations in the breeding program of pangolagrass. The purpose of this study is to examine the somaclonal variation of regenerated plants by means of phenotypic characters survey and RAPD analysis. After long-term suspension culture and EMS mutagenesis, many regenerated plants with morphological variations have been found. Among the regenerants, 10 clones with obviously different characteristics were selected for further studies. Nine of them showed significant differences in morphological trains as compared to A254. By …

Phylogenetics Of Apomictic Common Dallisgrass (Paspalum Dilatatum), B L. Burson, M A. Hussey

IGC Proceedings (1977-2023)

Common dallisgrass, Paspalum dilatatum Poir., an important warm season forage grass, is an obligate apomict with 50 chromosomes which associate as 20 bivalents and 10 univalents during meiosis. Because efforts to improve the grass have not been successful, a phylogenetic investigation was initiated to identify the progenitors of common dallisgrass in an effort to circumvent the apomictic barrier to improvement. The genomic composition has been determined for four dallisgrass biotypes: yellow-anthered (2n=4x=40) IIJJ; common (2n=5x=50) IIJJX; Uruguayan (2n=6x=60) IIJJXX; and Uruguaiana (2n=6x=60) IIJJXX. While the source of the X genome is unknown, the genes controlling apomixis are on at least …

Increased Longevity Of Red Clover Through Selection For Persistence And Disease Resistance, R R. Smith

IGC Proceedings (1977-2023)

Red clover (Trifolium pratense L.), an excellent perennial forage legume, has been considered for many years to be a short-lived, two to three years, forage. Selection for resistance to foliar and root rot diseases and for longevity of stand over the past four decades has improved the persistence of the currently available cultivars. The present studies were conducted to evaluate the progress from selection for longevity and disease resistance. Selection for stand longevity and improved disease resistance has reliably extended the production of red clover into the third and fourth year. Selection for healthy plants in 3- or …

Backcrosses Of Diploid Lolium-Festuca Hybrids To Festuca, W Nitzche

IGC Proceedings (1977-2023)

Fertile diploid hybrids of Lolium x Festuca -backcrosses were produced by colchicine-treatment without chromosome doubling. The hybrids have new combinations of their characters, not known in the natural species.