Digital Commons Network^™

Phyllochron Development In Cool-Season Forage Grasses, A B. Frank, J D. Berdahl, J F. Karn

IGC Proceedings (1977-2023)

The purpose of this study was to evaluate the relationship between grass leaf insertion rate and accumulated growing degree-days, and determine the phyllochron for five perennial forage grass species and two cultivars of each species. Species field seeded in solid stands were crested wheatgrass [Agropyron desertorum (Fisch. ex. Link) Schult.], intermediate wheatgrass, [Thinopyrum intermedium Barkw. & Dewey:Syn:A. intermedium (Host) Brauv], western wheatgrass [Pascopyrum smithii (Rybd) L ve], green needlegrass (Stipa viridula Trin.), and smooth bromegrass (Bromus inermis Leyss). Species phyllochron differences ranged from 77 GDD for Mandan 404 smooth bromegrass to 114 GDD for Lodorm …

Response To Drought Of White Clover Lines Selected For Different Stolon Morphologies, H D. Karsten, J R. Caradus, D R. Woodfield

IGC Proceedings (1977-2023)

White clover (Trifolium repens L.) lines were selected from within large and small-leaved cultivars of Grasslands Kopu and Grasslands Tahora, respectively, for long or short internodes, and for high or low branching frequency from plants grown in sun and shade (50% full sunlight). Lines were compared for drought tolerance in a perennial ryegrass (Lolium perenne L.) sward in boxes. Prior to imposing drought branching frequency selections did not differ in branching frequency, although the low branching frequency selection had a higher percentage of rooted nodes. After an imposed drought treatment sun-selected lines grew better than shade-selected lines relative …

Kentucky Bluegrass Floral Induction And Cultivar Response To Mechanical Removal Of Harvest Residue, G A. Murray, J B. Swensen

IGC Proceedings (1977-2023)

Open-field burning of post-harvest residue from Kentucky bluegrass (Poa pratensis L.) seed fields is being phased out in Washington, USA and may be banned in Idaho. Burning encourages early fall regrowth and timely completion of fall floral induction. Cultivars have different floral induction requirements and respond differently to mechanical residue removal. Our objective was to determine if length of fall floral induction requirement was related to seed yield when post-harvest residue was removed by crewcut vacuum sweeping. Floral induction requirements were not related to first-or second-year seed yields. Third-year seed yield of cultivars with long floral induction requirements declined …

The Influence Of Intensity Of Tree Thinning On The Redistribution Of Soil Water In Southern African Mopani Veld, G N. Smit, N F.G. Rethman, A Moore, A Le Roux, J S. Swart

IGC Proceedings (1977-2023)

The investigation was conducted on a densely wooded area in the Mopani veld of South Africa. Six plots were subjected to different intensities of tree thinning, ranging from a totally cleared plot (0 %) to plots thinned to the equivalent of 10%, 20%, 35%, 50% and 75% of the leaf biomass of a control plot (100%). Soil water measurements were taken at six different depths to a depth of 825 mm. The infiltration of rain water and redistribution within the soil profile exhibited marked differences between experimental plots. The soil water was predominantly held at a very shallow depth ( …

Relationship Of Visual And Quantitative Methods Of Grass Sward Development, R B. Mitchell, L E. Moser, K J. Moore

IGC Proceedings (1977-2023)

The objective of this study was to determine the relationship between visual and quantitative estimates of the morphological development of perennial grass swards. Pure stands of intermediate wheatgrass [Thinopyrum intermedium (Host) Barkw. & D.R. Dewey] and switchgrass (Panicum virgatum L.) were hand-clipped to ground level at 2-wk intervals in 1991 at Mead, NE, morphologically classified as mean stage count (MSC), and visually estimated for sward development. Visual estimations of sward development for both species were representative of quantitative measurements during vegetative growth. However, as sward development advanced to the elongation and heading stages, visual methods over-estimated the population …

Morphological Development Rates Of Perennial Forage Grasses, R B. Mitchell, L E. Moser, K J. Moore, D D. Redfearn

IGC Proceedings (1977-2023)

The objective of this study was to determine the rate of change in the morphological development of switchgrass (Panicum virgatum L.) and big bluestem (Andropogon gerardii Vitman). Pure stands of each species were sampled at weekly intervals in 1990 and 1991 at Mead, NE, and morphologically classified as mean stage count (MSC) and mean stage weight (MSW). Linear day of the year equations accounted for 94% of the variation in switchgrass MSC and MSW. Switchgrass MSC and MSW increased at an average rate of 0.0204 and 0.0234 units per day, respectively. Linear day of the year equations accounted …

Fertilizer Nitrogen And Morphogenetic Response In Avena Sativa And Lolium Multiflorum, F Lattanzi, M A. Marino, A Mazzanti

IGC Proceedings (1977-2023)

A field experiment was carried out at the EEA Balcarce, INTA, Argentina (37° 45’LS, 58° 18’LW) to determine whether Leaf Appearance Rate (LAR) was affected by N fertilization in Italian ryegrass (Lolium multiflorum Lam.) and forage oats (Avena sativa). N treatments (0, 50, 100, 150, 200 and 250 kg N ha-1) were applied in winter 1995, after a defoliation. Subsequently, number of leaves per tiller was determined on 45 labelled tillers in each treatment twice a week. LAR was calculated as the slope of the linear regression of number of leaves on thermal time (air temperature, base …

Allocation Of Carbon-14 To Roots Of Different Ages In Perennial Ryegrass (Lolium Perenne L.), C Matthew, W D. Kemball

IGC Proceedings (1977-2023)

The objective of this study was to provide information on how current photosynthate allocated to the root system in perennial ryegrass (Lolium perenne L.) is distributed among individual roots at differing nodal positions. Mature single ryegrass tillers were transplanted in September 1993 to glass house pots and three months later four of the plants were supplied with 14CO2. Individual roots from these radioactively-labeled plants were identified according to their nodal position on the tiller axis and amount of radiocarbon in each root quantified for root tip segments and for the remainder of the root axis. Similar plants were destructively …

The Analysis Of Dynamic Interaction In Legume Binary Mixture Under Controlled Conditions Of Irrigation And Clipping, M S. Vrahnakis, B Noitsakis, Z Koukoura

IGC Proceedings (1977-2023)

The objective of this study was to analyse the type of interference that occurred between the annual legume species, purple clover (Trifolium purpureum L.) and narrow leaved crimson clover (Trifolium angustifolium Loisel. ), growing in mixed conditions under two different watering regimes and two different clipping treatments. A replacement series experiment was conducted in pots placed in the field. The above ground biomass (gr/plant) were measured. The recently proposed Inverse Linear Model was implied in order to analyse the competitive interaction between the above species. The results suggest that Tr. purpureum was the superior competitor to Tr. angustifolium …

Increased Perceived Confidence In Professional Role Skills Among Undergraduate Dietetic Students Following Simulation-Based Learning Experiences, Makenzie Barr-Porter, Elizabeth Combs, Lauren Batey, Dawn Brewer, Aaron Kyle Schwartz, Tammy Stephenson

UK CARES Faculty Publications

Simulation-based learning experiences (SBLEs) are effective for teaching healthcare students clinical and communication skills. The current study assessed self-perceived clinical and communication confidence among dietetics students completing a series of four SBLEs (3 group, 1 individual) across nine months. Dietetics students were recruited in February 2023 prior to their first SBLE. Simultaneously through the academic year, students completed clinical and communication courses. Students were invited to complete an online, anonymous self-reported survey regarding confidence with nutrition care and communication prior to their first SBLE (Time 1), prior to their third SBLE (Time 2), and following their final SBLE (Time 3). …

Forage News [2024-04], University Of Kentucky

Forage News

No abstract provided.

Neurotensin Modulates Ovarian Vascular Permeability Via Adherens Junctions, Andrew Pearson, Ketan Shrestha, Thomas E. Curry, Diane M. Duffy

UK CARES Faculty Publications

Neurotensin (NTS) is a 13-amino acid peptide which is highly expressed in the mammalian ovary in response to the luteinizing hormone surge. Antibody neutralization of NTS in the ovulatory follicle of the cynomolgus macaque impairs ovulation and induces follicular vascular dysregulation, with excessive pooling of red blood cells in the follicle antrum. We hypothesize that NTS is an essential intrafollicular regulator of vascular permeability. In the present study, follicle injection of the NTS receptor antagonist SR142948 also resulted in vascular dysregulation. To measure vascular permeability changes in vitro, primary macaque ovarian microvascular endothelial cells (mOMECs) were enriched from follicle aspirates …

Impact Of Burnout And Professional Fulfillment On Intent To Leave Among Pediatric Physicians: The Findings Of A Quality Improvement Initiative, R. Thomas Collins Ii, Aric Schadler, Hong Huang, Scottie B. Day, John A. Bauer

UK CARES Faculty Publications

Background and Objectives Physician burnout is rampant, and physician retention is increasingly hard. It is unclear how burnout impacts intent to leave an organization. We sought to determine how physician burnout and professional fulfillment impact pediatric physicians’ intent to leave (ITL) an organization.

Design and Methods We performed 120, 1:1 semi-structured interviews of our pediatric faculty and used the themes therefrom to develop a Likert-scale based, 22-question battery of their current work experience. We created a faculty climate survey by combining those questions with a standardized instrument that assesses burnout and professional fulfillment. We surveyed pediatric and pediatric-affiliated (e.g. pediatric …

Paradox Lost: Wide Gape In The Ordovician Brachiopod Rafinesquina Explains How Unattached Filter-Feeding Strophomenoids Thrived On Muddy Substrates, Benjamin F. Dattilo, Rebecca L. Freeman, Kyle Hartshorn, David Peterman, Aaron Morse, David L. Meyer, Lindsay G. Dougan, James W. Hagadorn

Earth and Environmental Sciences Faculty Publications

Strophomenoid brachiopods had thin, concavo-convex shells, were ubiquitous colonizers of Palaeozoic muddy seafloors, and are hypothesized to have filter-fed in a concave-upward orientation. This orientation would elevate their line of commissure out of potentially lethal lophophore-clogging mud. The paradox is that epi- biont distributions on strophomenoids support a convex-upward life position, as do studies of strophome- noid stability and trace fossils formed by strophomenoid sediment-clearing. A premise of the concave-upward orientation hypothesis is a narrow gape, which causes narrow, high velocity inhalant currents, leaving strophomenoids vul- nerable to sediment entrainment. Herein we investigate the gape angle of Rafinesquina using serial …

Rethinking Wind In Kentucky, Lawrence E. Holloway, Aron Patrick, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

Recent analyses and developments suggest that wind energy could play a role in Kentucky's future power generation mix. This recent change in outlook for Kentucky wind has been driven by three factors: (1) improved wind turbine technologies, (2) improved economics, and (3) recent analyses showing improved grid reliability due to wind's complementarity to solar power generation.

Platelet-Derived Thrombospondin 1 Promotes Immune Cell Liver Infiltration And Exacerbates Diet-Induced Steatohepatitis, Taesik Gwag, Sangderk Lee, Zhenyu Li, Alana Newcomb, Josephine Otuagomah, Steven A. Weinman, Ying Liang, Changcheng Zhou, Shuxia Wang

UK CARES Faculty Publications

Background & Aims: Recent studies have implicated platelets, particularly a-granules, in the development of non-alcoholic steatohepatitis (NASH). However, the specific mechanisms involved have yet to be determined. Notably, thrombospondin 1 (TSP1) is a major component of the platelet a-granules released during platelet activation. Hence, we aimed to determine the role of platelet-derived TSP1 in NASH.

Methods: Platelet-specific Tsp1 knockout mice (TSP1Dpf4 ) and their wild-type littermates (TSP1 F/F) were used. NASH was induced by feeding the mice with a diet enriched in fat, sucrose, fructose, and cholesterol (AMLN diet). A human liver NASH organoid model was also employed.

Results: Although …

Joint Environmental And Social Benefits From Diversified Agriculture, Laura Vang Rasmussen, Ingo Grass

Earth and Environmental Sciences Faculty Publications

Agricultural simplification continues to expand at the expense of more diverse forms of agriculture. This simplification, for example, in the form of intensively managed monocultures, poses a risk to keeping the world within safe and just Earth system boundaries. Here, we estimated how agricultural diversification simultaneously affects social and environmental outcomes. Drawing from 24 studies in 11 countries across 2655 farms, we show how five diversification strategies focusing on livestock, crops, soils, noncrop plantings, and water conservation benefit social (e.g., human well-being, yields, and food security) and environmental (e.g., biodiversity, ecosystem services, and reduced environmental externalities) outcomes. We found that …

Willingness To Utilize A Mobile Treatment Unit In Five Counties At The Epicenter Of The Us Rural Opioid Epidemic., Snigdha R. Peddireddy, Melvin D. Livingston Iii, April M. Young, Patricia R. Freeman, Umedjon Ibragimov, Kelli A. Komro, Michelle R. Lofwall, Carrie B. Oser, Michele Staton, Hannah L. F. Cooper

Epidemiology and Environmental Health Faculty Publications

INTRODUCTION: US federal policies are evolving to expand the provision of mobile treatment units (MTUs) offering medications for opioid use disorder (MOUD). Mobile MOUD services are critical for rural areas with poor geographic access to fixed-site treatment providers. This study explored willingness to utilize an MTU among a sample of people who use opioids in rural Eastern Kentucky counties at the epicenter of the US opioid epidemic.

METHODS: The study analyzed Cross-sectional survey data from the Kentucky Communities and Researchers Engaging to Halt the Opioid Epidemic (CARE2HOPE) study covering five rural counties in the state. Logistic regression models investigated the …

Southeastern Law Librarian Spring 2024, Seaall

Newsletters

No abstract provided.

Open Educational Resources In Kentucky, Stephen G. Krueger, Todd Seguin, Colleen Deel, Aj Boston, Lidiya Grote, Bailey Lake, Katrina Salley, Stacy Scott, Kelly Smith, Maria Taylor, Kathleen Richardson

Library Faculty and Staff Publications

Open educational resources (OER) play an increasingly important role in the education landscape, with increased awareness and use year over year (Coffey). Often, academic libraries play a supporting role for instructors as they locate, adopt, and create OER for their courses. In this article, we will provide an introduction to OER, outline some current trends in open education, and describe a few of the OER initiatives currently underway in Kentucky’s college and university libraries.

Are Transformative Agreements Worth It? An Analysis Of Open Access Publication Data At The University Of Kentucky, Ben Rawlins

Library Faculty and Staff Publications

Open access publishing is continuing to grow as funders such as cOAlition S, National Institutes of Health, and the White House implement mandates and requirements that publicly funded research be made immediately available for public consumption. Publishers have adopted open access as a business model through transformative agreements that combine subscription and publishing fees. However, it is unclear whether these agreements are beneficial for libraries. This article discusses a project by the University of Kentucky Libraries to gather and analyze open access publication data to aid in the evaluation of transformative agreement proposals. This article also discusses how the University …

Guidebook For Project Management, Jill Asher, Keith Caudill, Travis Thompson, Jeff Jasper, Chris Van Dyke, Samantha Wright, Bryan Gibson, Pam Clay-Young, Candice Wallace

Kentucky Transportation Center Research Report

For the Kentucky Transportation Cabinet’s (KYTC) Department of Highways, project development encompasses all the work activities needed to move a transportation project from concept and project initiation to construction letting. Developing projects efficiently requires the adoption of sound project management practices. Although KYTC has a long track record of executing complex projects, doing so is increasingly challenging due to staff attrition and the attendant loss of institutional knowledge. Today’s entry- and mid-level project managers have more responsibilities than ever but lack a trusted source of guidance to help them manage projects from development through completion. The objective of this study …

Reducing Vehicle Licensing And Titling Fraud, Mallory Brown, Andrew Martin, Jeeyen Koo, Jennifer Walton

Kentucky Transportation Center Research Report

This study reviews Kentucky’s vehicle licensing and registration program, evaluates methods of motor vehicle title fraud, examines current anti-fraud mechanisms in Kentucky, reports on vehicle titling and anti-fraud efforts in other states, assesses Ohio’s vehicle inspection program, and develops a proposal for a new Kentucky rebuilt title inspection program. Researchers developed the proposal based on historical Kentucky vehicle registration and titling data, a survey of other states, and other information provided by Kentucky’s Division of Motor Vehicle Licensing and other agency partners. Kentucky historical data revealed consistently high numbers of salvage title applications as well as spatial concentration in rebuilt …

A Multi-Ancestry Genetic Study Of Pain Intensity In 598,339 Veterans., Sylvanus Toikumo, Rachel Vickers-Smith, Zeal Jinwala, Heng Xu, Divya Saini, Emily E Hartwell, Mirko Pavicic, Kyle A Sullivan, Ke Xu, Daniel A Jacobson, Joel Gelernter, Christopher T Rentsch, Million Veteran Program, Eli Stahl, Martin Cheatle, Hang Zhou, Stephen G Waxman, Amy C Justice, Rachel L Kember, Henry R Kranzler

Epidemiology and Environmental Health Faculty Publications

Chronic pain is a common problem, with more than one-fifth of adult Americans reporting pain daily or on most days. It adversely affects the quality of life and imposes substantial personal and economic costs. Efforts to treat chronic pain using opioids had a central role in precipitating the opioid crisis. Despite an estimated heritability of 25-50%, the genetic architecture of chronic pain is not well-characterized, in part because studies have largely been limited to samples of European ancestry. To help address this knowledge gap, we conducted a cross-ancestry meta-analysis of pain intensity in 598,339 participants in the Million Veteran Program, …

Effects Of The Communities That Heal (Cth) Intervention On Perceived Opioid-Related Community Stigma In The Healing Communities Study: Results Of A Multi-Site, Community-Level, Cluster-Randomized Trial, Alissa Davis, Hannah K. Knudsen, Daniel M. Walker, Deborah Chassler, Karsten Lunze, Philip M. Westgate, Emmanuel Oga, Sandra Rodriguez, Sylvia Tan, Janae Holloway, Sharon L. Walsh, Carrie B. Oser, R. Craig Lefebvre, Laura C. Fanucchi, Lashawn Glasgow, Ann Scheck Mcalearney, Hilary L. Surratt, Michael W. Konstan, Terry T-K Huang, Patricia Lebaron, Julie Nakayima, Michael D. Stein, Maria Rudorf, Monica Nouvong, Elizabeth N. Kinnard, Nabila El-Bassel, Jess Tilley, Aaron Macoubray, Caroline Savitzky, Amy Farmer, Donna Beers, Pamela Salsberry, Timothy R. Huerta

Behavioral Science Faculty Publications

BACKGROUND: Community stigma against people with opioid use disorder (OUD) and intervention stigma (e.g., toward naloxone) exacerbate the opioid overdose crisis. We examined the effects of the Communities that HEAL (CTH) intervention on perceived opioid-related community stigma by stakeholders in the HEALing Communities Study (HCS).

METHODS: We collected three surveys from community coalition members in 66 communities across four states participating in HCS. Communities were randomized into Intervention (Wave 1) or Wait-list Control (Wave 2) arms. We conducted multilevel linear mixed models to compare changes in primary outcomes of community stigma toward people treated for OUD, naloxone, and medication for …

Barriers To Opioid Use Disorder Treatment Among People Who Use Drugs In The Rural United States: A Qualitative, Multi-Site Study., T. J. Stopka, A. T. Estadt, G. Leichtling, J. C. Schleicher, L. S. Mixson, J. Bresett, E. Romo, P. Dowd, S. M. Walters, A. M. Young, W. Zule, P. D. Friedmann, V. F. Go, R. Baker, R. J. Fredericksen

Epidemiology and Environmental Health Faculty Publications

BACKGROUND: In 2020, 2.8 million people required substance use disorder (SUD) treatment in nonmetropolitan or 'rural' areas in the U.S. Among this population, only 10% received SUD treatment from a specialty facility, and 1 in 500 received medication for opioid use disorder (MOUD). We explored the context surrounding barriers to SUD treatment in the rural United States.

METHODS: We conducted semi-structured, in-depth interviews from 2018 to 2019 to assess barriers to SUD treatment among people who use drugs (PWUD) across seven rural U.S. study sites. Using the social-ecological model (SEM), we examined individual, interpersonal, organizational, community, and policy factors contributing …

Interaction Between Continuous Pack-Years Smoked And Polygenic Risk Score On Lung Cancer Risk, Meredith S. Duncan, Hector Diaz-Zabala, James Jaworski, Hilary A. Tindle, Robert A. Greevy, Loren Lipworth, Rayjean J. Hung, Matthew S. Freiberg, Melinda C. Aldrich

Biostatistics Faculty Publications

Background: Lung cancer risk attributable to smoking is dose dependent, yet few studies examining a polygenic risk score (PRS) by smoking interaction have included comprehensive lifetime packyears smoked. Methods: We analyzed data from participants of European ancestry in the Framingham Heart StudyOriginal (n = 454) andOffspring (n = 2,470) cohorts enrolled in 1954 and 1971, respectively, and followed through 2018. We built a PRS for lung cancer using participant genotyping data and genome-wide association study summary statistics froma recent study in theOncoArray Consortium. We used Cox proportional hazards regression models to assess risk and the interaction between pack-years smoked and …

Genetic Associations With Dementia-Related Proteinopathy, Yuriko Katsumata, David W. Fardo, Lincoln M.P. Shade, Xian Wu, Shama D. Karanth, Timothy J. Hohman, Julie A. Schneider, David A. Bennett, Jose M. Farfel, Kathryn Gauthreaux, Charles Mock, Walter A. Kukull, Erin L. Abner, Peter T. Nelson, Maria Carrillo, Eric M. Reiman, Kewei Chen, Donna Masterman, Robert C. Green, Carole Ho, Adam Fleisher, Andrew J. Saykin, Kwangsik Nho, Liana G. Apostolova, Shannon L. Risacher, Jonathan Jackson

Biostatistics Faculty Publications

INTRODUCTION: Although dementia-related proteinopathy has a strong negative impact on public health, and is highly heritable, understanding of the related genetic architecture is incomplete. METHODS: We applied multidimensional generalized partial credit modeling (GPCM) to test genetic associations with dementia-related proteinopathies. Data were analyzed to identify candidate single nucleotide variants for the following proteinopathies: Aβ, tau, α-synuclein, and TDP-43. RESULTS: Final included data comprised 966 participants with neuropathologic and WGS data. Three continuous latent outcomes were constructed, corresponding to TDP-43-, Aβ/Tau-, and α-synuclein-related neuropathology endophenotype scores. This approach helped validate known genotype/phenotype associations: for example, TMEM106B and GRN were risk alleles …

Neurological Diagnoses In Hospitalized Covid-19 Patients Associated With Adverse Outcomes, Meghan R Hutch, Jiyeon Son, Trang T Le, Chuan Hong, Xuan Wang, Zahra Shakeri Hossein Abad, Michele Morris, Alba Gutiérrez-Sacristán, Jeffrey G Klann, Anastasia Spiridou, Ashley Batugo, Riccardo Bellazzi, Vincent Benoit, Clara-Lea Bonzel, William A Bryant, Lorenzo Chiudinelli, Kelly Cho, Priyam Das, Tomás González González, David A Hanauer, Darren W Henderson, Yuk-Lam Ho, Ne Hooi Will Loh, Adeline Makoudjou, Simran Makwana, Alberto Malovini, Bertrand Moal

Biostatistics Faculty Publications

Few studies examining the patient outcomes of concurrent neurological manifestations during acute COVID-19 leveraged multinational cohorts of adults and children or distinguished between central and peripheral nervous system (CNS vs. PNS) involvement. Using a federated multinational network in which local clinicians and informatics experts curated the electronic health records data, we evaluated the risk of prolonged hospitalization and mortality in hospitalized COVID-19 patients from 21 healthcare systems across 7 countries. For adults, we used a federated learning approach whereby we ran Cox proportional hazard models locally at each healthcare system and performed a meta-analysis on the aggregated results to estimate …

Antisense Oligonucleotide Targeting Hepatic Serum Amyloid A Limits The Progression Of Angiotensin Ii-Induced Abdominal Aortic Aneurysm Formation, Preetha Shridas, Ailing Ji, Andrea C. Trumbauer, Victoria P. Noffsinger, Luke W. Meredith, Frederick C. De Beer, Adam E. Mullick, Nancy R. Webb, Dennis G. Karounos, Lisa R. Tannock

Saha Cardiovascular Research Center Faculty Publications

Background and aims: Obesity increases the risk for abdominal aortic aneurysms (AAA) in humans and enhances angiotensin II (AngII)-induced AAA formation in C57BL/6 mice. We reported that deficiency of Serum Amyloid A (SAA) significantly reduces AngII-induced inflammation and AAA in both hyperlipidemic apoE-deficient and obese C57BL/6 mice. The aim of this study is to investigate whether SAA plays a role in the progression of early AAA in obese C57BL/6 mice.

Methods: Male C57BL/6J mice were fed a high-fat diet (60% kcal as fat) throughout the study. After 4 months of diet, the mice were infused with AngII until the end …