Digital Commons Network^™

Lifetime Interpersonal Violence Or Abuse And Diabetes Rates By Sex And Race, Maureen Sanderson, Mekeila Cook, L. Lauren Brown, Veronica Mallett, Ann L. Coker

Obstetrics and Gynecology Faculty Publications

Introduction: Lifetime exposure to interpersonal violence or abuse has been associated with several chronic diseases, including adult-onset diabetes, yet this pattern has not been confirmed by sex and race within a large cohort.\Methods: Data from the Southern Community Cohort Study collected between 2002−2009 and 2012−2015 were used to explore the relationship between lifetime interpersonal violence or abuse and diabetes (N=25,251). Prospective analyses of lower-income people living in the southeastern U.S. were conducted in 2022 to examine the risk of adult-onset diabetes associated with lifetime interpersonal violence or abuse by sex and race. Lifetime interpersonal violence or abuse was defined as …

Cd47 Antisense Oligonucleotide Treatment Attenuates Obesity And Its-Associated Metabolic Dysfunction, Taesik Gwag, Dong Li, Eric Ma, Zhenheng Guo, Ying Liang, Shuxia Wang

Saha Cardiovascular Research Center Faculty Publications

Previous study from our lab has revealed a new role of CD47 in regulating adipose tissue function, energy homeostasis and the development of obesity and metabolic disease in CD47 deficient mice. In this study, the therapeutic potential of an antisense oligonucleotide (ASO) targeting to CD47 in obesity and its‑associated complications was determined in two obese mouse models (diet induced and genetic models). In diet induced obesity, male C57BL6 mice were fed with high fat (HF) diet to induce obesity and then treated with CD47ASO or control ASO for 8 weeks. In genetic obese mouse model, male six‑week old ob/ob mice …

Platelet Glycogenolysis Is Important For Energy Production And Function, Kanakanagavalli Shravani Prakhya, Hemendra J. Vekaria, Daniёlle M. Coenen, Linda Omali, Joshua Lykins, Smita Joshi, Hammodah Rawhi Hammodah Alfar, Qing Jun Wang, Patrick G. Sullivan, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Although the presence of glycogen in platelets was established in the 1960s, its importance to specific functions (i.e., activation, secretion, aggregation, and clot contraction) remains unclear. Patients with glycogen storage disease often present with increased bleeding and glycogen phosphorylase (GP) inhibitors, when used as treatments for diabetes, induce bleeding in preclinical studies suggesting some role for this form of glucose in hemostasis. In the present work, we examined how glycogen mobilization affects platelet function using GP inhibitors (CP316819 and CP91149) and a battery of ex vivo assays. Blocking GP activity increased glycogen levels in resting and thrombin-activated platelets and inhibited …

The Complementary Roles Of Vamp-2, -3, And -7 In Platelet Secretion And Function, Smita Joshi, Kanakanagavalli Shravani Prakhya, Alexis N. Smith, Harry Chanzu, Ming Zhang, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Platelet secretion requires Soluble N-ethylmaleimide Sensitive Attachment Protein Receptors (SNAREs). Vesicle SNAREs/Vesicle-Associated Membrane Proteins (v-SNAREs/ VAMPs) on granules and t-SNAREs in plasma membranes mediate granule release. Platelet VAMP heterogeneity has complicated the assessment of how/if each is used and affects hemostasis. To address the importance of VAMP-7 (V7), we analyzed mice with global deletions of V3 and V7 together or platelet-specific deletions of V2, V3, and global deletion of V7. We measured the kinetics of cargo release, and its effects on three injury models to define the context-specific roles of these VAMPs. Loss of V7 minimally affected dense and α …

Best3-Mediated Mekk2/3 Activation: A Novel Therapeutic Target In Aortopathies, Tatiana Zyrianova, Benjamin Lopez, Kathlyn Zou, Charles Gu, Dayna Pham, Sriharsha Talapaneni, Christopher M. Waters, Riccardo Olcese, Andreas Schwingshackl

Saha Cardiovascular Research Center Faculty Publications

Influenza-A virus (IAV) infects yearly an estimated one billion people worldwide, resulting in 300,000–650,000 deaths. Preventive vaccination programs and antiviral medications represent the mainstay of therapy, but with unacceptably high morbidity and mortality rates, new targeted therapeutic approaches are urgently needed. Since inflammatory processes are commonly associated with measurable changes in the cell membrane potential (Em), we investigated whether Em hyperpolarization via TREK-1 (K2P 2.1) K þ channel activation can protect against influenza-A virus (IAV)-induced pneumonia. We infected mice with IAV, which after 5 days caused 10–15% weight loss and a decrease in spontaneous activity, representing a clinically relevant infection. …

Deep Learning, 3d Ultrastructural Analysis Reveals Quantitative Differences In Platelet And Organelle Packing In Covid-19/Sarscov2 Patient-Derived Platelets, Sagar S. Matharu, Cassidy S. Nordmann, Kurtis R. Ottman, Rahul Akkem, Douglas Palumbo, Denzel R. D. Cruz, Kenneth Campbell, Gail Sievert, Jamie L. Sturgill, James Zachary Porterfield, Smita Joshi, Hammodah Rawhi Hammodah Alfar, Chi Peng, Irina D. Pokrovskaya, Jeffrey A. Kamykowski, Jeremy P. Wood, Beth A. Garvy, Maria A. Aronova, Sidney W. Whiteheart, Richard D. Leapman, Brian Storrie

Saha Cardiovascular Research Center Faculty Publications

Platelets contribute to COVID-19 clinical manifestations, of which microclotting in the pulmonary vasculature has been a prominent symptom. To investigate the potential diagnostic contributions of overall platelet morphology and their α-granules and mitochondria to the understanding of platelet hyperactivation and micro-clotting, we undertook a 3D ultrastructural approach. Because differences might be small, we used the high-contrast, high-resolution technique of focused ion beam scanning EM (FIB-SEM) and employed deep learning computational methods to evaluate nearly 600 individual platelets and 30 000 included organelles within three healthy controls and three severely ill COVID-19 patients. Statistical analysis reveals that the α-granule/ mitochondrion-to-plateletvolume ratio …

Lrp1 Protects Against Excessive Superior Mesenteric Artery Remodeling By Modulating Angiotensin Ii–Mediated Signaling, Jackie M. Zhang, Dianaly T. Au, Hisashi Sawada, Michael K. Franklin, Jessica J. Moorleghen, Deborah A. Howatt, Pengjun Wang, Brittany O. Aicher, Brian Hampton, Mary Migliorini, Fenge Ni, Adam E. Mullick, Mashhood M. Wani, Areck A. Ucuzian, Hong S. Lu, Selen C. Muratoglu, Alan Daugherty, Dudley K. Strickland

Saha Cardiovascular Research Center Faculty Publications

Vascular smooth muscle cells (vSMCs) exert a critical role in sensing and maintaining vascular integrity. These cells abundantly express the low-density lipoprotein receptor–related protein 1 (LRP1), a large endocytic signaling receptor that recognizes numerous ligands, including apolipoprotein E–rich lipoproteins, proteases, and protease-inhibitor complexes. We observed the spontaneous formation of aneurysms in the superior mesenteric artery (SMA) of both male and female mice in which LRP1 was genetically deleted in vSMCs (smLRP1–/– mice). Quantitative proteomics revealed elevated abundance of several proteins in smLRP1–/– mice that are known to be induced by angiotensin II–mediated (AngII-mediated) signaling, suggesting that this pathway was dysregulated. …

Macrophage Angiotensin-Converting Enzyme Reduces Atherosclerosis By Increasing Peroxisome Proliferator-Activated Receptor Α And Fundamentally Changing Lipid Metabolism, Duoyao Cao, Zakir Khan, Xiaomo Li, Suguru Saito, Ellen A. Bernstein, Aaron R. Victor, Faizan Ahmed, Aoi O. Hoshi, Luciana C. Veiras, Tomohiro Shibata, Mingtian Che, Lei Cai, Michifumi Yamashita, Ryan E. Temel, Jorge F. Giani, Daniel J. Luthringer, Ajit S. Divakaruni, Derick Okwan-Duodu, Kenneth E. Bernstein

Saha Cardiovascular Research Center Faculty Publications

Aims The metabolic failure of macrophages to adequately process lipid is central to the aetiology of atherosclerosis. Here, we examine the role of macrophage angiotensin-converting enzyme (ACE) in a mouse model of PCSK9-induced atherosclerosis.

Methods and results Atherosclerosis in mice was induced with AAV-PCSK9 and a high-fat diet. Animals with increased macrophage ACE (ACE 10/10 mice) have a marked reduction in atherosclerosis vs. WT mice. Macrophages from both the aorta and peritoneum of ACE 10/10 express increased PPARα and have a profoundly altered phenotype to process lipids characterized by higher levels of the surface scavenger receptor CD36, increased uptake of …

Investigating Visinin-Like Protein 1 And Ubiquitinated Visinin-Like Protein 1 As Mild Traumatic Brain Injury Biomarkers, Yueming Wu

Theses and Dissertations--Chemistry

Traumatic brain injury (TBI) continues to be a significant cause of morbidity and mortality worldwide. Despite significant progress in understanding the complex pathophysiology of TBI, the underlying mechanisms remain poorly understood. The primary brain damage is acute and irreversible. However, secondary brain injuries often develop gradually over months to years, creating an opportunity for critical therapeutic interventions. In the past decade, research on TBI biomarkers has seen significant progress. This progress has been driven by the diverse nature of TBI pathologies and the challenges they present for evaluation, management, and prognosis. TBI biomarker proteins resulting from axonal, neuronal, or glial …

“Thinking Across Bodies”: Percepción Woolfiana Y Giro Material En La Cuentística De Rodoreda, Roig, Alós Y Riera, Ana Álvarez Guillén

Theses and Dissertations--Hispanic Studies

Conventional understandings of perception have long undergirded traditional readings of Modernist texts in adopting a predominantly subject-centered perspective that separates subject from object in a vertical and hierarchical relationship. I argue that a consideration of Virginia Woolf’s short stories in dialogue with four generations of twentieth-century Catalan women writers who followed her work closely suggests an entirely different epistemological framework of perception in which subject and object are fluidly and horizontally organized. Mercè Rodoreda, Concha Alós, Montserrat Roig and Carme Riera establish a horizontal fictional dialogue that constitutes a return to matter that decenters the subject, resulting in an alternative …

Alzheimer’S Disease Genetics And Short-Chain Fatty Acid Treatment In Studies Of The Murine Gut Microbiome, Diana Zajac

Theses and Dissertations--Physiology

Elucidating the relationship of the gut microbiome in Alzheimer's Disease (AD) risk and pathogenesis is an area of intense interest. Since 60 to 80% of AD risk is related to genetics and APOE alleles represent the most impactful genetic risk factors for AD, their mechanism(s) of action are under intense scrutiny.

First, I conducted a study on APOE targeted replacement mice to investigate the impact of APOE alleles on the murine gut microbiome. The relative abundance of bacteria from the family Ruminococacceae and related genera increased with APOE2 status. The relative abundance of the class Erysipelotrichia increased with APOE4 status, …

Peer-To-Peer Energy Trading In Smart Residential Environment With User Behavioral Modeling, Ashutosh Timilsina

Theses and Dissertations--Computer Science

Electric power systems are transforming from a centralized unidirectional market to a decentralized open market. With this shift, the end-users have the possibility to actively participate in local energy exchanges, with or without the involvement of the main grid. Rapidly reducing prices for Renewable Energy Technologies (RETs), supported by their ease of installation and operation, with the facilitation of Electric Vehicles (EV) and Smart Grid (SG) technologies to make bidirectional flow of energy possible, has contributed to this changing landscape in the distribution side of the traditional power grid.

Trading energy among users in a decentralized fashion has been referred …

Examining Migration Flows Across Kentucky's Counties, James Brady Stein

MPA/MPP/MPFM Capstone Projects

The state of Kentucky is home to many rural counties which experience high levels of outward migration due to their relatively unfavorable economic conditions. While migration trends nationally have begun to plateau, migration flows from county to county show a much more volatile story. This study will examine the relationship between economic opportunity and migration flow estimates in Kentucky’s counties through a multiple regression approach with the response variable being annual migration flow estimates, with multiple predictor variables showing the economic composition of the county. Variables used in this regression include annual unemployment rates, educational attainment levels, county poverty rates, …

Estill County "Twin Cities" Consolidation Study, Logan Hart

MPA/MPP/MPFM Capstone Projects

To provide essential services without imposing an unreasonable financial burden on their residents, local governments, particularly those with a declining population or tax base, are seeking ways to maximize their resources. Municipal consolidation is one of the solutions that many local governments have considered. This study aimed to determine if municipal consolidation would improve the efficiency and financial standing of the Estill County, Kentucky cities of Irvine and Ravenna. The study's findings suggest that consolidation could lead to cost benefits for both cities. However, due to the limited availability of data, further research is required to gain a complete understanding …

An Analysis Of Test Scores In The Central And Eastern Time Zones, Rliey Reed

MPA/MPP/MPFM Capstone Projects

During daylight savings in Kentucky, elementary students in the Eastern Time Zone receive around an hour less of sunlight than students in the Central Time Zone when attending school in the morning. With recent discussion of making daylight savings permanent, it's important to discuss the effects that going to school in the dark has on students' test scores. Research has been done to study the effects that operating on less amounts of sunlight has on school aged children. This study seeks to do the same, by comparing test scores of 3rd grade students on the Central and Eastern Time Zones …

Did The Covid-19 Pandemic Cause A Learning Loss In Reading And Mathematics? Empirical Evidence Using 2022 Grade 8 Students In Kentucky., Tiyani Rodrigo

MPA/MPP/MPFM Capstone Projects

In 2020, most United States (U.S.) schools closed abruptly. Due to the pandemic, students and teachers were affected in multiple ways. Nearly three years post-pandemic, it is evident students lost in academic achievement. Lower academic performance may impact the future economy and labor force due to lower productivity. Therefore, understanding the causal impact COVID-19 had on academic achievement is vital to develop policy that will help mitigate the long-term impact. In this capstone, I will estimate the size and direction of the impact COVID-19 had on Mathematics and Reading skills for grade 8 students in Kentucky. Using a Difference-in-Differences (DiD) …

Budget Models And Methods Used In Higher Education, Brad West

MPA/MPP/MPFM Capstone Projects

The Cambridge Dictionary defines a budget as “a plan to show how much money a person or organization will earn and how much they will need or be able to spend.” (Budget, 2023). The budget of an institution within higher education is how to allocate the planned amount of revenues received to support the planned expenditures across the organization for an upcoming period, usually a fiscal year or project duration. Various public higher education institutions have many different methods for allocating those planned revenues to support expenditures across their colleges, schools, and administrative units to support the university's mission. This …

Navigating The Maze Of Student Loan Debt: Exploring Long-Term Economic Implications And Strategies For Alleviating The Burden, Kimberly Faulk

MPA/MPP/MPFM Capstone Projects

This capstone paper explores the long-term economic implications of student loan debt on individuals and society and identifies potential strategies that can be implemented to alleviate the burden. Student loan debt has become a significant issue globally, affecting individuals of all backgrounds and having far-reaching consequences for both individuals and the broader economy. The analysis reveals that the long-term economic implications of student loan debt on individuals are multifaceted. High levels of debt can hinder financial stability and economic mobility, as borrowers struggle to save, invest, and participate in the broader economy. The burden of student loan debt can delay …

Bourbon Barrel Tax, William Twigg

MPA/MPP/MPFM Capstone Projects

This paper will look at the impacts of barrel tax reform within the state of Kentucky and the impact of the sunsetting of such an important revenue generator for the Commonwealth given the recent passing of Kentucky House Bill 5 (HB5) on March 31, 2023. It will provide a brief history of the bourbon industry, how Kentucky became known as the homeland of bourbon, some background as to what the barrel tax is and why the tax was needed in the first place, and provide some context as to how the tax was assessed, how much it amounted to, and …

Public Health Access And Service For All, Austin Nugent

MPA/MPP/MPFM Capstone Projects

Collecting, analyzing, and reporting accurate, reliable data is necessary for achieving health equity. Although data alone will not lead to equitable health outcomes for all people, it helps health professionals, researchers, and policymakers understand health disparities and develop effective interventions, programs, and policies to reduce them. Necessary data includes disaggregated health information on diverse demographics, including people with disabilities. However, despite this need, disability data gaps exist. Fortunately, numerous solutions exist to address this issue, including implementing minimum data standards on disability and engaging all levels of health organizations in data practices.

This capstone project investigated the current data practices …

Accurate Oxygen Abundance Of Interstellar Gas In Mrk 71 From Optical And Infrared Spectra, Yuguang Chen, Tucker Jones, Ryan Sanders, Dario Fadda, Jessica Sutter, Robert Minchin, Erin Huntzinger, Peter Senchyna, Daniel Stark, Justin Spilker, Benjamin J. Weiner, Guido Roberts-Borsani

Physics and Astronomy Faculty Publications

The heavy element content (“metallicity”) of the Universe is a record of the total star formation history. Gas-phase metallicity in galaxies, as well as its evolution with time, is of particular interest as a tracer of accretion and outflow processes. However, metallicities from the widely-used electron temperature (Te) method are typically ∼ 2× lower than the values based on the recombination line method. This “abundance discrepancy factor” (ADF) is well known and is commonly ascribed to bias due to temperature fluctuations. We present a measurement of oxygen abundance in the nearby (3.4 Mpc) system, Mrk 71, using a combination of …

Excitation And Ionization Properties Of Star-Forming Galaxies At Z = 2.0-9.3 With Jwst/Nirspec, Ryan L. Sanders, Alice E. Shapley, Michael W. Topping, Naveen Reddy, Gabriel B. Brammer

Physics and Astronomy Faculty Publications

We utilize medium-resolution JWST/NIRSpec observations of 164 galaxies at z = 2.0-9.3 from the Cosmic Evolution Early Release Science (CEERS) survey to investigate the evolution of the excitation and ionization properties of galaxies at high redshifts. Our results represent the first statistical constraints on the evolution of the [O III]/Hβ versus [N II]/Hα, [S II]/Hα, and [O I]/Hα “BPT” diagrams at z > 2.7, and the first analysis of the O₃₂ versus R₂₃ diagram at z > 4 with a large sample. We divide the sample into five redshift bins containing 30-40 galaxies each. The subsamples at z ∼ 2.3, …

Wilcoxon-Mann-Whitney Effects For Clustered Data: Informative Cluster Size, Changrui Liu

Theses and Dissertations--Statistics

In recent research, there has been a growing interest in understanding the impact of informative cluster size (ICS) on statistical inference for clustered data. In the non-parametric context, the problem for testing equality of distribution functions has been the main consideration. We are aiming to develop inferential procedures for the Wilcoxon-Mann-Whitney effect, also known as the non-parametric relative effect, involving two or more groups. Computationally, results from both simulated and real-world data have shown promising results that our proposed tests effectively account for ICS and they particularly outperform other methods in the literature designed for ignorable cluster sizes. The applications …

Estimating Bedrock Shear Wave Velocity At Vsap, Paducah, Kentucky, Using Ambient Noise, Russel Rogers

Theses and Dissertations--Earth and Environmental Sciences

Due to the nature of the Mississippi Embayment region and its vicinity to the New Madrid Seismic Zone, earthquake ground-motion site effects are potential hazards and quantifying the associated hazard is a necessary step for public safety. Measuring the V_s contrast between bedrock and overlying soil layers is necessary for site effect evaluation. Various methodologies for investigating shear wave velocity (V_s) profiles have been implemented in the Mississippi Embayment. However, these previous studies provided a wide range of bedrock Vs. In this study, microtremor array measurements, utilizing ambient background noise, were used to provide an additional estimate …

The Supine Moving Apprehension Test—Reliability And Validity Among Healthy Individuals And Patients With Anterior Shoulder Instability, Alon Rabin, Ofir Chechik, Margie K. Olds, Timothy L. Uhl, Efi Kazum, Adin Deutsch, Eran Citron, Tal Cohen, Oleg Dolkart, Assaf Bibas, Eran Maman

Physical Therapy Faculty Publications

Background: Performance-based tests for patients with anterior shoulder dislocation are lacking. This study determined the reliability and validity of the supine moving apprehension test designed to assess the ability to control anterior instability loads. Methods: Thirty-six participants were recruited (18 healthy individuals, and 18 patients following anterior shoulder dislocation). Healthy participants performed the supine moving apprehension test on 2 separate occasions to determine test-retest reliability. Patients completed the supine moving apprehension test and the Western Ontario Shoulder Instability index before and 6 months after surgical stabilization of their shoulder. The presence of anterior apprehension was also documented post-operatively. Results: The …

Expectile Neural Networks For Genetic Data Analysis Of Complex Diseases, Jinghang Lin, Xiaoran Tong, Chenxi Li, Qing Lu

Biostatistics Faculty Publications

The genetic etiologies of common diseases are highly complex and heterogeneous. Classic methods, such as linear regression, have successfully identified numerous variants associated with complex diseases. Nonetheless, for most diseases, the identified variants only account for a small proportion of heritability. Challenges remain to discover additional variants contributing to complex diseases. Expectile regression is a generalization of linear regression and provides complete information on the conditional distribution of a phenotype of interest. While expectile regression has many nice properties, it has rarely been used in genetic research. In this paper, we develop an expectile neural network (ENN) method for genetic …

Musical Portraiture In Acht Korte Karakterschetsen Voor Piano Vierhandig By Hans Osieck, Yuri Kim

Theses and Dissertations--Music

Hans Osieck (1910-2000) was a Dutch composer, pianist, and teacher. His musical portraits, Acht Korte Karakterschetsen voor piano vierhandig (Eight Short Character Sketches for Four Hands), are a valuable addition to the piano duo repertoires for intermediate and advanced students. In Eight Sketches, Osieck musically portrays eight of his students using simple compositional techniques, much like the brushstrokes and color palette of an artistic portrait. As a result of their programmatic nature and relatable subjects, Osieck’s Eight Sketches can be particularly useful for students developing their imaginations and learning to perform expressively. As a piano duo, Eight Sketches also serves …

Opportunities For Cantabile Performance In Seung Ki Hong’S Arirang Variations, Hye Jin Yeom

Theses and Dissertations--Music

The purpose of this project is introducing South Korean composer Seung Ki Hong’s Arirang Variations (2015). Hong’s post-2013 work has focused on incorporating traditional Korean melodies into Western instrumental music; Arirang Variations exemplifies this process. The theme is “Arirang,” a folksong that Koreans have sung to express their joys and sorrows for centuries. Hong transforms this iconic melody through a series of variations in various dance styles such as a tango and a Viennese waltz.

This paper includes two main sections. The first provides background information that, although important for understanding Arirang Variations, is inaccessible to many performers. This section …

From A Humble Cry To A Holy Chaconne: The Background, Analysis, And Performance Guide To Chaconne For Tokkae, Op. 96 By M. W. Johann Kim, Jurang Kim

Theses and Dissertations--Music

The cries of animals have been used as motivic material in classical music literature throughout history. This project considers the Cambodian tocay gecko’s cry as the motif which embodies the harmonic structure in Chaconne for Tokkae for Violin and Piano, Op. 96 by Dr. Myung Whan Johann Kim. It reveals how Dr. Kim adapts unique compositional style and technique such as the bell harmony of Dr. Kurt Anton Hueber, to develop the theme into 39 colorful variations. The essential elements of the chaconne and the composer’s intentions are examined through personal interviews and correspondence with the composer. These elements are …

Julie Giroux’S Symphony No. 6: The Blue Marble Background, Analysis, And Conductor’S Guide, Josh Gillen

Theses and Dissertations--Music

Julie Giroux’s Symphony No. 6: The Blue Marble (2022) is an original work for wind band that is quickly rising in popularity among instrumental conductors and ensembles throughout the world. The purposes of this document are to

1) provide biographical details regarding Julie Giroux and her musical career;

2) provide a background of the work’s conception from a musical and multi-media perspective through interviews with Giroux and the first conductors of the work;

3) provide a multi-parametric structural analysis of the work and its companion film;

4) provide conductors with useful information for programming and rehearsing the work.

Chapter one …