Digital Commons Network^™

Industry To Classroom: The Role Of Academic Program Consultants In Building Self-Efficacy Among Occupation-Based Career And Technical Education Teachers, Erica L. Settelen

Theses and Dissertations--Educational Leadership Studies

Occupation-based career and technical education (CTE) teachers bring industry expertise to secondary classrooms but often begin teaching without traditional pedagogical preparation or familiarity with public school systems. In Kentucky’s New Teacher Institute (NTI), academic program consultants (APCs) provide program-area support to strengthen induction. Using the framework of self-efficacy and guided by Ivankova’s mixed methods action research (MMAR) framework, this study examined how occupation-based CTE teachers perceived APC engagement during induction and how APC-created asynchronous supports were experienced.

In the Reconnaissance Phase, a sequential Quantitative → Qualitative design included an initial survey followed by interviews. Survey results indicated midrange familiarity with …

From The Ivory Tower To The Town Square: Defining The Public Scholar, Mary K. Littlepage

Theses and Dissertations--Educational Policy Studies and Evaluation

The term public scholar has gained increasing attention among academics and institutions, yet it lacks a definition grounded in empirical methods. This has left the term open to interpretation and led to inconsistencies in its application. Existing definitions are theoretical and emphasize a public scholar’s effort to bridge the gap between research and practice. My aim for this study was to conceptualize and preliminarily operationalize the term public scholar as a construct drawing on qualitative and quantitative data. Through a thematic analysis and initial Rasch scaling, I provided clear parameters around the construct specifically highlighting a public scholar’s behavior (practices), …

Unveiling The Shadows: An Exploratory Study On Ghostwriting, Gray Markets, And The Ethics Of Contract Cheating, Micah Crawford

Theses and Dissertations--Educational Policy Studies and Evaluation

Contract cheating has become an increasingly complex challenge for higher education, particularly as digital platforms, global academic labor markets, and generative artificial intelligence reshape the production and detection of academic work. While much of the existing literature examines contract cheating from the perspectives of students, faculty, and institutions, less attention has been given to the individuals who produce ghostwritten academic work. This dissertation addresses that gap by exploring how shadow scholars understand their roles, ethical boundaries, and participation within the academic integrity ecosystem.

Using an exploratory qualitative design, this study draws on twelve semi-structured narrative interviews with six freelance academic …

Effects Of Yoga And Mindfulness On Emotional Regulation, Behavior, And Teacher Burnout, Sara E. Smith

Theses and Dissertations--Education Sciences

This dissertation comprises three studies that examine the effects of physical activity, yoga, and mindfulness interventions on students' emotional regulation, behavior, and academic outcomes in rural Kentucky schools. These studies as a whole contribute to understanding how movement-based and mindfulness practices can enhance social-emotional and academic well-being in schools.

The first study describes the effects of classroom-based physical activity on student achievement and social-emotional well-being. Forty-five elementary students participated in a 6-week intervention with two 7-minute movement breaks per day. Results indicated statistically significant improvements in Reading (t(44) = 2.55, p < .001, d = .38), Math (t(44) = 4.63, p < .001, d = .69), and social-emotional competence (t(44) = 3.67, p < .001, d = .55). These findings demonstrate that brief, structured physical activity can meaningfully enhance academic and emotional outcomes when implemented by classroom teachers.

The second study shows the effects of a five-week …

Exploring The Relationships Between Social Influence, Vaping Behaviors, And Susceptibility Across Grade Levels In Kentucky, Raphael Adesiyan, Odunayo O. Omotilewa, Saber Feizy, Melinda J. Ickes

Kinesiology and Health Promotion Faculty Publications

Purpose: This study explored the relationships between social influence, vaping behavior, and susceptibility across different grade levels.   Design: The study design was cross-sectional. Data were collected from middle and high school students who voluntarily participated between September 2022 and April 2023.   Sample: A convenience sample of students in 6^th to 12^th grades in Kentucky (N = 4677).   Measures: Demographic information, including age, grade level, school location, and race/ethnicity, was collected. The outcome variables included social influence, susceptibility, and vaping behavior.   Analysis: Logistic regression analysis was used to explore the relationships between social influence, vaping behavior, and susceptibility.   Results: …

Academic Departments As Microfoundations Of Institutional Governance, Karley A. Riffe, Meghan J. Pifer

Educational Policy Studies and Evaluation Faculty Publications

Considerations of governance in US higher education do not typically include the academic department. This results in a knowledge gap about the role of these central organizing units of academic work in the governance of colleges and universities. Through the concept of microfoundations, we explore concrete ways in which departmental governance matters, how it may be challenging, and strategies for fostering effective governance within academic departments.

Bringing Pandemic Science To The Classroom: Building Public Health Capacity At A Rural Kentucky High School, Sahar Alameh, Anna G. Hoover, James W. Keck, Scott M. Berry, Sagan Goodpaster, Savannah Tucker, Ashley Goodin

Science, Technology, Engineering, and Mathematics (STEM) Education Faculty Publications

In response to the COVID-19 pandemic, a multidisciplinary team at the University of Kentucky developed an interdisciplinary science, technology, engineering, and mathematics and environmental health unit—the Wastewater Assessment for Coronavirus in Kentucky: Implementing Enhanced Surveillance Technology (WACKIEST) Unit—for high school students in summer 2022. This case study outlines the WACKIEST Unit, which focused on wastewater surveillance and COVID-19, the obstacles faced during development and recruitment, and implementation of the WACKIEST Unit in conjunction with a rural wastewater surveillance initiative. The unit was implemented in spring 2023 at a rural high school in Kentucky, spanning 12 days and engaging 190 students. …

Chinese Athletes’ And Parents’ Motivations For U.S. Collegiate Sports Participation, Wenting Sun, Russell D. Ravert, Simran Kaur Sethi

Kinesiology and Health Promotion Faculty Publications

To gain an understanding of international student recruitment and retention in collegiate athletics, this study examines the motivations and decision-making processes of Chinese high school athletes and their parents in pursuing U.S. collegiate sport opportunities. Using the push–pull framework and an ecological perspective, it explores how individual aspirations, family priorities, institutional resources, and national policies shape transnational mobility. Semistructured focus group discussions with 10 athletes and eight parents revealed six key motivational themes and highlighted the influence of recent collegiate sport-related policy changes in China. The findings suggest that while both groups value U.S. sport pathways, athletes and parents may …

Emergency Department-Initiated Standard Versus High-Dose Buprenorphine Induction (Envision): A Randomised Clinical Trial Protocol, Kathryn Hawk, Andrew Herring, Marek Chawarski, Erik S. Anderson, Michael Baumann, Alyrene Dorey, August F. Holtyn, Christopher Jones, Shara Martel, Patricia Owens, Kaitlin Kmiecik, Tania D. Strout, Peter Taillac, Michelle R. Lofwall, Sharon L. Walsh, Gail D’Onofrio

Center on Drug and Alcohol Research Faculty Publications

Introduction: The initiation of buprenorphine for patients with opioid use disorder (OUD) in the emergency department (ED) has been associated with improved outcomes including reduced ED visits and increased treatment engagement. Though both standard-dose (8 mg buprenorphine equivalent) and high-dose (24 mg buprenorphine equivalent) strategies to initiate buprenorphine have been used in the ED, no prospective trials comparing outcomes among patients receiving these treatments have been reported.

Methods and analysis: This multisite randomised clinical trial is a multisite double-blind, double-dummy, randomised clinical trial enrolling 360 emergency department patients with moderate-to-severe OUD. Enrolled patients will be randomised to one of two …

Experiential Spillover In Public Views Of Police And Courts, Emma Henderson, Matthew Mettler, Jeffery J. Mondak, Mark Peffley

Political Science Faculty Publications

Personal experiences with public officials influence how those officials are perceived. We question whether these experiential effects extend to other officials. If people draw on what they know to make inferences about unfamiliar evaluative targets, then interactions with one actor may shape evaluations of other independent actors. Focusing on police and courts, analyses of data from two surveys reveal that personal interactions with public officials produce spillover effects on appraisals of officials in different sectors. The findings indicate that experiential spillover constitutes an underappreciated force in public opinion, one that operates outside the control of the actors being evaluated.

Facebook As A Strategy To Deliver A Peer Navigation Intervention For Recently Incarcerated Women With Opioid Use Disorder: A Mixed Methods Descriptive Profile, Martha Tillson, Jaxin Annett, Marguerite A. Webster, Amanda Fallin-Bennett, Mandi Webster, Amber Clemons, Cynthia Robinson, Kevin Crabtree, Casey Baker-Romans, Michele Staton

Center on Drug and Alcohol Research Faculty Publications

Recovery support and treatment linkages are critical during transitions from incarceration to community but can be challenging to deliver to hard-to-reach populations. This paper will examine Facebook contacts between peer navigators (PNs) and women with opioid use disorder (OUD) following jail release to explore women’s use of PN services, including purpose and content of Facebook messages. As part of a larger clinical trial under the Justice Community Opioid Innovation Network (JCOIN), PNs provided OUD treatment navigation and recovery support for 12 weeks following women’s release from jail, using remote options such as Facebook. Facebook Messenger transcripts between PNs and participants …

Evolution Of A Client‐Level Dataset: 20 Years Of National Child Traumatic Stress Network Data Collection, C. A. Purbeck, A. N. Cooke, L. J. Liang, C. Smith, J. Staten, J. Epstein, H. B. Hodgdon, Jessica Eslinger, A. Wells, J. Goodrich, T. H. Bethel, K. Willett, Y. C. Fu, J. A. Fairbank, E. C. Briggs

Center on Trauma and Children Faculty Publications

Objectives: For 20 years, the National Child Traumatic Stress Network's (NCTSN) data collection efforts have been a central mechanism for Clinical Quality Improvement (ClQI). The NCTSN is a federally funded initiative in the United States composed of programs dedicated to raising the standard of care and increasing access to services for children and families who have experienced traumatic events.

Methods: These efforts span three initiatives: the Core Data Set (CDS), the Clinical Improvement through Measurement Initiative (CIMI) Measurement Based Care (MBC), and CIMI Data Sharing (CIMI-DS) that collected and analyzed client-level data to improve care for children and families served …

Contemporary Commercial Music: A Scoping Review With Implications For Music Therapy Practice, John A. Lawwill

Theses and Dissertations--Music

Contemporary Commercial Music (CCM) is an increasingly popular group of non-classical musical styles. Music therapists need to know how to sing a wide variety of CCM genres authentically, yet little information exists on effectively teaching vocal CCM techniques to music therapists. The purpose of this study was to conduct a scoping review on CCM singing instruction and to synthesize implications for music therapists. I used EBSCOhost and ProQuest to search for reports in English published after 2002 containing the terms “Contemporary Commercial Music” or “CCM,” and variations of the term “sing.” I identified 26 reports that met inclusion criteria, including …

Belting In Musical Theatre: Vocal Pathologies, Injuries, And Pedagogical Implications, Kaylee Piatt

Theses and Dissertations--Music

Belting has become one of the most defining and contested vocal styles in contemporary musical theatre. Valued for its dramatic immediacy, speech-like intensity, and ability to cut through amplified orchestration, belting is frequently associated with heightened injury risk and pedagogical controversy. This thesis examines the relationship between belting, vocal pathology, and pedagogy through an interdisciplinary lens that integrates voice science, clinical literature, and professional performance practice. Rather than framing belting as an inherently harmful or stylistically aberrant technique, this study conceptualizes belting as a functional vocal coordination shaped by physiological mechanisms, cumulative vocal load, and occupational demands.

Drawing on existing …

Cantate Fratres: Forming And Sustaining Traditional Catholic Music, Liturgy And Culture In The Institute Of Christ The King Sovereign Priest, Reyers P. Brusoe

Theses and Dissertations--Music

The Institute of Christ the King Sovereign Priest is an understudied member of the Traditional Catholic and Latin Mass Communities whose unique approach to Catholic liturgy, culture, and formation offers a sustainable model for the Catholic Church's longevity and renewal. This study is the first of its kind, combining the theoretical positions of affect theory, ecomusicology/musical sustainability, and the musicology of religion to explore and explain the success of this growing classical community. This dissertation examines Church documents, theological literature, and multidisciplinary academic sources as a foundation for the study.             In studying the Institute of Christ the King Sovereign Priest, …

Burnout And Mindful Self-Care Practices Among Bipoc Women Music Therapists: A Cross-Sectional Survey, Melissa V. Peters

Theses and Dissertations--Music

Music therapy as a profession has long acknowledged burnout as a significant occupational concern, yet the experiences of marginalized therapists remain understudied. In particular, little research has explored how burnout and self-care manifest for those who identify as Black, Indigenous, or People of Color (BIPOC) women in a field historically dominated by white women. Most of the existing literature on this topic is focused on the majority identity of music therapists, white women. The purpose of this thesis was to examine the reported levels of burnout among BIPOC women music therapists as well as their reported frequency of engagement in …

Cultus: Eco-Phenomenology In The Work Of John Steinbeck, Robinson Jeffers, And Jack London, Carter D. Johnson

Theses and Dissertations--English

In the face of increasingly dire environmental crises, proposed solutions have gravitated toward imperatives for faster, cleaner, and more efficient technologies and resources. While practical solutions are necessary, they are entirely insufficient to deal with an underlying issue driving environmental degradation: unbridled instrumental reason. In this dissertation, I suggest a theoretical posture, cultus, which attempts to balance the necessity of instrumental action with the guiding counterweight of receptivity. Furthermore, I argue that such personal receptivity is uniquely fostered in aesthetic experiences, attuning the individual to the non-identical and renewing a relationship of care.

I examine the life and work of …

Sylvia Plath, Ted Hughes, And The Question Of The “True” Ariel: Complicating Binaries In Plath’S Literary Legacy, Jacob C. Quinn

Theses and Dissertations--English

This thesis aims to complicate common binaries that have defined critical responses to Sylvia Plath’s Ariel since its first publication in 1965, and which emerged as the result of her husband Ted Hughes’ alterations to her original selection and arrangement of poems. Hughes’ editing emphasizes a narrative which ends in bleak fatality, whereas Plath’s original narrative concludes on a note of bittersweet hope. I read Hughes’ choice of ending as highly influential on Plath’s reception and legacy. The tone of her immediate reception, largely shaped by Al Alvarez, characterized Plath as an “extremist” poet whose work should be read through …

We Need To Care About Care: (Un)Caring Relations In Victorian Fiction, Roslyn Fleming

Theses and Dissertations--English

The professionalization and institutionalization of care in the Victorian era ushered in a growing societal concern about the pitfalls of care being “systematized and depersonalized” and treating people as burdens, problems to be solved. Given the increasing attention paid to people’s welfare and well-being during this period, the cultural shift from intimate community care to professional and institutional care, and the growing stigmatization of disabled bodyminds, the fiction produced amidst these cultural changes makes a compelling site for a study of care. Critical scholarship attending to Victorian novels’ representations of care, however, has largely concentrated on specific categorizations of care …

The Impact Of Congenital Heart Disease On The Timing Of Alzheimer's Disease In Down Syndrome, Julianne G. Clina, Brian C. Helsel, Sigan L. Hartley, David A. White, Victoria L. Fleming-Batayneh, Benjamin Handen, Bradley Christian, Elizabeth Head, Mark Mapstone, Christy L. Hom, Beau Ances, Jeffrey Burns, H. Diana Rosas, Florence Lai, Sharon Krinsky Mchale, Joseph H. Lee, Frederick A. Schmitt, Jordan Harp, Ira T. Lott, Shahid Zaman, Lauren T. Ptomey

Neurology Faculty Publications

INTRODUCTION: The incidence of Alzheimer's disease (AD) in Down syndrome (DS) exceeds 90%. Approximately 50% of people with DS have congenital heart disease (CHD). Having CHD increases risk for early-onset AD in populations without DS, but it is unclear if CHD influences AD in DS. METHODS: Data from the Alzheimer Biomarker Consortium-Down Syndrome (ABC-DS) were used. Participants with CHD (n = 82, mean age = 39.9 ± 8.5 years, 97.6% White race) were age- and sex-matched to participants without CHD (n = 82, mean age = 40.5 ± 8.1 years, 98.8% White race). Cognitive assessments and Centiloid load (CL) (positron …

Plasma P-Tau217 And Apoe Genotype, Ann M. Stowe, Bilal Kahn, Ali Ballesteros, Monique Cortez, Linda S. Hynan, Victoria Cantrell, Indu Raman, Guangchun Chen, Eric D. Vidoni, Ellen Binder, Jeffrey Burns, Jeffrey N. Keller, Diana R. Kerwin, Tristyn Hall, Pavel Yanev, Wanpen Vongapatanasin, David C. Zhu, Norman Scheel, C. Munro Cullum, Rong Zhang, Dwight C. German

Neurology Faculty Publications

Introduction: Plasma phosphorylated tau (p-tau)217 levels and apolipoprotein E (APOE) ε4 genotype are used for prodromal identification of individuals at high risk for dementia. We used baseline samples from cognitively normal subjects in the Risk Reduction for Alzheimer's Disease (rrAD) clinical trial (NCT02913664) to investigate the relationship between these two markers in older adults with additional vascular risk factors. Methods: We measured APOE genotype, plasma p-tau217, and standard neuroimaging metrics in 400 rrAD participants, aged 60 to 84 years, with sedentary lifestyle, hypertension, and hypercholesterolemia. Results: Plasma p-tau217 was 57% higher in subjects with at least one APOE ε4 allele, …

Decoding The Human Pbmc Isonome, Patricia Hayes Doyle, Madeline L. Page, J. Anthony Brandon, Bernardo Aguzzoli Heberle, Brendan J. White, Ann Marie Stowe, Mark T.W. Ebbert

Neurology Faculty Publications

Long-read single-cell RNA sequencing provides an opportunity to understand human health and disease at a level difficult to resolve with bulk or short-read methods. This approach enables isoform-level investigation of cellular diversity and disease mechanisms and definition of cell-types, rather than using genes alone. Using a modified, microfluidic-free PIPseq workflow and computational pipeline adapted for Oxford Nanopore long-read sequencing, we generated the largest long-read single-cell dataset of human peripheral blood mononuclear cells (PBMCs) from a single donor to date, the first with sufficient cell numbers to detect megakaryocytes. This study profiled isoform usage across immune cells, integrating marker expression and …

Post-Approval Study 3-Year Outcomes Of The Neuroform Atlas Stent For The Treatment Of Wide-Necked Intracranial Aneurysms, Brian Jankowitz, Ashutosh P. Jadhav, Bradley Gross, Tudor G. Jovin, Abdulnasser A. Alhajeri, Justin F. Fraser, Ricardo A. Hanel, Eric Sauvageau, Amin Aghaebrahim, Donald Frei, Richard Bellon, David N. Loy, Ajit S. Puri, Adel M. Malek, Ajith J. Thomas, Gabor Toth, Demetrius Klee Lopes, R. Webster Crowley, John Reavey-Cantwell, Eugene Lin, Adnan Siddiqui, Michael J. Alexander, Ahmad Khaldi, Geoffrey P. Colby, Justin M. Caplan, Sudhakar R. Satti, Aquilla S. Turk, Alejandro M. Spiotta, Richard Klucznik, Danial K. Hallam, David Kung, Michael T. Froehler

Neurology Faculty Publications

Introduction The ATLAS trial was a prospective, multicenter, single-arm, investigational device exemption (IDE) study to evaluate the safety and effectiveness of the Neuroform Atlas Stent System for the treatment of widenecked bifurcation aneurysms. This analysis presents the 36-month follow-up data for the anterior and posterior cohorts. Methods Of the 182patients in the IDE anterior cohort, 146 consented to participate in the post-approval study (PAS) and, of the 116patients in the IDE posterior cohort, 101 consented to participate in the PAS. The primary effectiveness endpoint was core laboratory adjudicated (Raymond–Roy 1; RR1) without retreatment or parent artery stenosis (>50%) at …

Climate Change Effects On Biomass And Greenhouse Gas Emissions Are Ameliorated By Nontoxic Endophytes In Southeastern Usa Transition Zone Tall Fescue Pastures, Rebecca Kay Mcgrail, Jonathan D. Moore, A. Elizabeth Carlisle, Jim A. Nelson, Rebecca L. Mcculley

Plant and Soil Sciences Faculty Publications

Background: The plant–microbe interaction between tall fescue and its endophyte Epichloë coenophiala can affect ecosystem responses to changing rainfall patterns and increasing temperatures. Endophyte-infected (E+) stands often exhibit improved stress tolerance and differing soil greenhouse gas (GHG) emissions compared to endophyte-free (E−) stands. However, it is unknown if mammalian nontoxic endophyte (NTE) strains introduced into some tall fescue cultivars confer differing stress tolerances or reduce soil GHG emissions.

Methods: We quantified the impact of fescue-NTE symbioses in Jesup and Texoma on biomass and GHG emissions in response to increased temperature (+3°C) and altered precipitation (50% lengthened interval between events) using …

A Global Synthesis Of Fragipan Silicon Concentrations And Relationships With Soil Physicochemical Properties As A Function Of Soil Parent Materials, Christopher Shepard

Plant and Soil Sciences Faculty Publications

Fragipans are dense, brittle subsurface horizons that restrict root growth and limit water-holding capacity. A common factor in these soils is a binding agent, potentially either phyllosilicate clay bridging, amorphous aluminosilicate/silica, or silica sorbed to Fe oxides. Others have suggested that no binding agent is present within these soil horizons and that the arrangement of individual particles is solely responsible for these horizons. In this article, a secondary data synthesis was utilized to demonstrate that silicon (Si) accumulation is a key pedogenic process in loess-derived fragipan horizons, but not in all fragipans. The average dithionite-extractable Si (Si_d) content …

Autosiq, Liming Zhou, Talukder Z. Jubery, Baskar Ganapathysubramanian, Thomas Lübberstedt, Siddique I. Aboobucker

Plant and Soil Sciences Faculty Publications

Doubled haploid (DH) technology can fast-track crop breeding.Haploid induction yields haploids with only one set of genomes, which are usually sterile.Haploid fertility (HF) is the ability of haploid plants to set seed, and it is a critical bottleneck in DH pipelines.Genetic mechanisms to restore HF hold immense potential in DH crop breeding, yet its phenotyping remains manual, destructive, and inconsistent.While recent advances in imaging and machine learning have improved throughput for general plant traits, no curated image dataset exists for Arabidopsis thaliana that explicitly represents HF.Here, we present AutoSiQ, a dataset and baseline deep learning pipeline for automated HF quantification.AutoSiQ …

Carbon Nanodot Precursors Enable Ultrahigh-Loading Copper Single-Atom Catalysts For Oxygen Reduction Reaction, Prakhar Sharma, Ayanthi Thisera, Matthew G. Boebinger, Jenna K. Rector, Jason M. Unrine, Beth S. Guiton, Doo Young Kim

Plant and Soil Sciences Faculty Publications

Single-atom catalysts (SACs) have attracted significant attention in electrocatalysis due to their high metal utilization and tunable electronic structures; however, their practical implementation is often limited by poor atomic dispersion, low metal loadings (typically < 1 wt%), and insufficient anchoring of single metal atoms on catalyst supports. Herein, we report a simple and versatile strategy to synthesize copper single-atom catalysts (Cu-SACs) with ultrahigh metal loading using citric-acid-derived carbon nanodots as metal-capturing precursors. Through hydrothermal treatment followed by pyrolysis in the presence of urea, atomically dispersed copper atoms coordinated with nitrogen are incorporated into a carbon framework, achieving a Cu loading of up to 20 wt% while maintaining atomic dispersion. Notably, a significant fraction of the copper single atoms exists as Cu⁺–N₂ coordination, which provides under-coordinated and catalytically active sites. The resulting Cu-SAC exhibits promising oxygen reduction reaction (ORR) activity in alkaline media, delivering a limiting current density comparable to that of commercial 20 wt% Pt/C with a predominant four-electron reduction pathway. Despite this high intrinsic activity, durability tests reveal gradual performance degradation under prolonged electrochemical operation, which is attributed to demetallation and aggregation of …

Separation Of Trichothecene Mycotoxins In A Matrix Of Hemp (Cannabis Sativa L.) Seed, And Quantification Of Deoxynivalenol In Hemp Grain Infected With Fusarium Graminearum, Isabelle A. Kagan, Magdalena Ricciardi, Henry S. Smith, Rachel R. Schendel, Robert Pearce, Nicole Gauthier

Plant and Soil Sciences Faculty Publications

Background: Fusarium graminearum, one of the causal agents of ear rot in maize and head blight of cereal grains, also infects hemp (Cannabis sativa L. containing a maximum of 0.3% tetrahydrocannabinol or THC). This fungal pathogen produces trichothecene mycotoxins when it infects maize and cereal grains, but little is known about its production of mycotoxins in hemp. A method was developed to separate mycotoxins deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON), and 3-acetyldeoxynivalenol (3-ADON) in commercial hemp seed and grain (harvested tissue), using high-performance liquid chromatography (HPLC) with ultraviolet detection. Results: In HPLC analysis of commercial hemp seed, DON was separated from matrix …

Hybrid Genome And Enzyme Characterization Uncover Novel Glycosyltransferases Associated With Benzylisoquinoline Alkaloid Chemodiversity In Lotus, Hedi Zhao, Xinyi Qi, Yan Liu, Zhuyin Chen, Yifan Wang, Jing Que, Yanyan Su, Ling Yuan, Gangqiang Dong, An Liu, Zhennan Wang, Sha Chen

Plant and Soil Sciences Faculty Publications

Introduction: Hybridization is a key evolutionary force that enriches genetic diversity and is associated with metabolic innovation. Lotus (Nelumbo), comprising Asian lotus (N. nucifera) and American lotus (N. lutea), is both a culturally important ornamental and medicinal plant rich in benzylisoquinoline alkaloids (BIAs). However, the genomic and biochemical basis of BIA glycoside biosynthesis remains poorly understood. Objectives: A high-quality hybrid lotus genome was presented to dissect the formation of benzylisoquinoline alkaloid glycosides and the mechanism underlying their synthesis. Methods: The chromosomal-level hybrid genome was constructed through PacBio HiFi, ONT Ultra-long, and Hi-C sequencing data combined assembly strategies. The metabolite and …

Characterizing The Human Metapneumovirus Matrix Protein And Its Role In Infection, Chase J. Heim

Theses and Dissertations--Molecular and Cellular Biochemistry

Human metapneumovirus (HMPV) causes severe respiratory tract infections in all cohorts, but especially in vulnerable groups such as children, older adults, and the immunocompromised. The matrix (M) protein of HMPV, like the M proteins of other members of the Mononegavirales order, is involved in virus assembly and budding. However, other functions of the HMPV M protein have yet to be elucidated. To investigate these various functions of the M protein, we used a peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) antisense agent designed to block translation of the M gene to reduce M expression during infection. Treatment with varying concentrations of an …