Digital Commons Network^™

Best Practices For Recruitment And Retention Of Transportation Professionals, Chris Van Dyke, Bryan Gibson, Sarah Mccormack, Merl Hackbart, Doug Kreis

Kentucky Transportation Center Research Report

Many public sector agencies, including state departments of transportation (DOTs), have long found recruiting and retaining staff a daunting challenge. Lured by the promise of higher salaries in the private sector, many people are reluctant to sacrifice their long-term earnings potential to pursue public service. And with the quality of fringe benefits (e.g., retirement plans, health insurance) offered by public agencies having declined gradually, it is even more difficult to persuade prospective employees to accept a lower salary. Many studies over the past 15—20 years have looked at methods for solving the problem of recruitment and retention within the context …

Spontaneous Alignment Of Myotubes Through Myogenic Progenitor Cell Migration, Lauren E. Mehanna, Adrianna R. Osborne, Charlotte A. Peterson, Brad J. Berron

Chemical and Materials Engineering Faculty Publications

In large-volume muscle injuries, widespread damage to muscle fibers and the surrounding connective tissue prevents myogenic progenitor cells (MPCs) from initiating repair. There is a clinical need to rapidly fabricate large muscle tissue constructs for integration at the site of large volume muscle injuries. Most strategies for myotube alignment require microfabricated structures or prolonged orientation times. We utilize the MPC’s natural propensity to close gaps across an injury site to guide alignment on collagen I. When MPCs are exposed to an open boundary free of cells, they migrate unidirectionally into the cell-free region and align perpendicular to the original boundary …

Local Strain Quantification Of A Porous Carbon Fiber Network Material, Robert N Quammen, Paul F. Rottmann

Chemical and Materials Engineering Faculty Publications

While porous materials’ wide range of attractive functional properties have led to their development for a variety of applications, their intrinsically stochastic microstructures prevent straightforward approaches to predicting their mechanical behavior. This is attributed to the mechanisms that govern the macroscale behavior of these materials operating on multiple microstructure-specific length scales spanning several orders of magnitude. The goal of this work was to experimentally observe these operative deformation mechanisms to better improve the development of mechanism-informed models that more accurately predict the behavior of these materials. In this study compression tests were conducted on a porous carbon fiber network material. …

Staphylococcus Aureus Oleate Hydratase Produces Ligands That Activate Host Ppara, Christopher D. Radka, Matthew W. Frank, Tyler S. Simmons, Cydney N. Johnson, Jason W. Rosch, Charles O. Rock

Markey Cancer Center Faculty Publications

Commensal gut bacteria use oleate hydratase to release a spectrum of hydroxylated fatty acids using host-derived unsaturated fatty acids. These compounds are thought to attenuate the immune response, but the underlying signaling mechanism(s) remain to be established. The pathogen Staphylococcus aureus also expresses an oleate hydratase and 10- hydroxyoctadecanoic acid (h18:0) is the most abundant oleate hydratase metabolite found at Staphylococcal skin infection sites. Here, we show h18:0 stimulates the transcription of a set of lipid metabolism genes associated with the activation of peroxisome proliferator activated receptor (PPAR) in the RAW 264.7 macrophage cell line and mouse primary bone marrow-derived …

Online Instructional Tools For Motor Carriers, Brian Howell, Jennifer Walton, Paul Ross, Andrew Martin

Kentucky Transportation Center Research Report

Commercial motor carrier customers on Kentucky highways must register and pay taxes for licensing and credentialing requirements. The Kentucky Transportation Cabinet (KYTC) provides an online Motor Carrier Portal that offers information and instructions on meeting these requirements, but customers have increasingly resorted to calling into KYTC’s motor carrier help desk, thereby straining limited personnel resources. The Kentucky Transportation Center (KTC) coordinated with KYTC to develop a series of online instructional videos that can better assist customers with applying for and meeting credentialing requirements.

KTC researchers used a three-phase approach to determine the feasibility and requirements for instructional videos. First, they …

Strengthening The Deployment Of Uncrewed Aerial Systems (Uas) At Kytc, Rachel Catchings, Suzanne Smith, Gayle Marks, Candice Wallace, Christopher Van Dyke

Kentucky Transportation Center Research Report

The Kentucky Transportation Cabinet (KYTC) and transportation agencies around the United States increasingly depends on uncrewed aircraft systems (UAS — also commonly known as drones) to collect data and accelerate project development and delivery. Drones let transportation practitioners safely and efficiently perform many activities, including bridge and highway inspection, construction monitoring, surveying, emergency response, and measuring stockpiles. KYTC’s implementation of UAS has expanded rapidly over the past 10 years, however, the agency lacks well-coordinated procedures for connecting pilots with end data users, scheduling and conducting flights, developing and documenting best practices, and processing and storing data. Based on a review …

Impact Of The New Context Functional Classifications For Kytc, Arlen Sandlin, Jill Asher, Nikiforos Stamatiadis, Jeff Jasper, Rachel Catchings, Chris Van Dyke

Kentucky Transportation Center Research Report

State transportation agencies are adopting an expanded context classification system to inform project development and delivery. This system classifies roadways into one of five categories based on factors such as level of development, building densities and setbacks, multimodal user patterns and requirements, network permeability, and speed. Compared to functional classification, context classification better captures the types of mobility, travel patterns, and user mixes observed in specific contexts. The expanded context classification system is found in AASHTO’s A Policy on Geometric Design of Highways and Streets (7^th Edition). The forthcoming 8^th edition will deepen integration of context classification throughout …

Pl 43 Traffic Forecasting Report 2023, Mei Chen, Xu Zhang, Eugene Antwi

Kentucky Transportation Center Research Report

This document is the seventh edition of the Traffic Forecasting Report (TFR) for the state of Kentucky. TFR data are important for multiple engineering applications that involve analyzing temporal and spatial variability in traffic distributions and volumes. Most of the metrics discussed in the report were computed using data from 2019, 2021, and 2022 — excluded from consideration were 2020 data because they exhibited anomalous traffic patterns tied to shutdowns associated with the COVID-19 pandemic. Data summarized in this report include average daily traffic, annual average daily truck traffic, travel activity by vehicle type, weekday and weekend hourly volume percentages, …

Characterization Of Chilling Sensitivity Of Tropical And Temperate Grasses, M Ebina, K Okumura, H Matsu-Ura, Y Tsurumi

IGC Proceedings (1977-2023)

C16-C18 fatty acid composition of some lipids in several species of temperate and tropical grasses were measured. The fatty acid composition of phosphatidylglycerol (PG) and sulfoquinovosyl diglyceride (SQDG) indicated significant differences between temperate and tropical grasses. Especially the differences of the fatty acid composition of PG were remarkable. The unsaturated fatty acid content of PG in tropical grasses was lower than the contents in temperate grasses. These differences constantly appeared through the year. These differences were caused by the low content of polyunsaturated fatty acids, or the low contents of C18 fatty acids.

Characterization Of Somatic Hybrids Between Festuca Arundinacea And Lolium Multiflorum, M Fujimori, T Takamizo, K Suginobu

IGC Proceedings (1977-2023)

Metabolically inactivated tall fescue (Festuca arundinacea Schreb.) protoplasts and X-ray irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts were electrofused resulting in the regeneration of twenty-six plantlets. Southern blot analysis using three mitochondrial probes and random amplified polymorphic DNA (RAPD) analysis revealed that the regenerants were intergeneric somatic hybrids. All regenerated plants were male sterile and some of them had stamens that exhibited the pistillode appearance.

Transformation In Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense Rna, R Akashi, T Uchiyama, Y Sakatani, O Kawamura

IGC Proceedings (1977-2023)

We have developed a rapid and reproducible transformation system for bird’s-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis.

Modeling Intake Of Grazing Cows Fed Complementary Feeds, D R. Buckmaster, L A. Holden, L D. Muller, R H. Mohtar

IGC Proceedings (1977-2023)

A model suitable for predicting intake for grazing or non-grazing dairy cows is presented. The model integrates the potentially intake limiting factors of physical fill, physiological energy demand, wet mass, herbage availability, herbage cover, and grazing time. Integration of these factors with a simple set of linear ration balancing constraints yields a model suitable for predicting supplemental feed requirements as well as potential animal production from a grazed land. The model is semi-theoretical, being descriptive in structure, but containing empirical relationships.

Research Programme To Improve The Utilization Of Home-Grown Forages In The Netherlands, A.M. Van Vuuren, A H. Bosma, G J. Kaspers, J.J. M.H. Ketelaars, E.N. Van Loo, H J.P. Marvin, H Valk, G.W.J. Van De Ven

IGC Proceedings (1977-2023)

In The Netherlands a research programme has been initiated by the Ministry of Agriculture, Nature Management and Fisheries to increase the proportion of high-quality home-grown forage in rations for dairy cows. This should result in a more sustainable dairy husbandry by improving the efficiency of utilization of nitrogen and phosphorus from home-grown forages (mainly grass and maize) on farm level. Research disciplines involved are plant breeding, forage harvesting and conservation and nutrition.

Rangeland-Centered Agroecosystems In Indian Central Himalayas: Operationalizing Ecological Sustainability In Mountain Agriculture, Vir Singh

IGC Proceedings (1977-2023)

Himalayan Mountains are home to unique agroecosystems harboring unique inter- and intra-species diversity in uncultivated and cultivated lands. The uncultivated lands are largely the rangelands which are ecologically more stable ecosystems than the cultivated lands which are ecologically prone areas. Rangelands, cultivated areas, and livestock in mountain agriculture are in organic linkages with each other, woven into a complex unitary whole, functionally oriented to produce foods and other life-supporting products – such as feed, fiber, fuel, fertilizers, etc. – and provide vital ecological functions. Rangelands in fragile Himalayan mountains serve as a key component of agriculture and the biodiversity conserved …

Mentoring Graduate Students For Academic Careers In Grassland Science, L. E. Sollenberger

IGC Proceedings (1977-2023)

Effective mentorship enhances student productivity and career achievements, yet faculty generally do not receive mentorship training, nor does mentorship receive focused attention, evaluation, and recognition. The objectives of this paper are to 1) define mentorship and identify roles of a mentor, 2) highlight qualities and outcomes of effective mentoring, and 3) elaborate best mentoring practices, metrics for assessing mentoring, and challenges for mentoring the next generation of grassland scientists. Mentoring is a professional alliance where individuals work together to support the personal and professional growth, development, and success of the relational partners. Mentors should be altruistic discipline experts, have substantial …

The Effect Of Nitrogen Rates And Plant Density On Grain Yield Components And Persistence In Intermediate Wheatgrass (Thinopyrum Intermedium) And Mountain Rye (Secale Strictum), M. T. Newell, N. Munday, R. C. Hayes

IGC Proceedings (1977-2023)

Intermediate wheatgrass (IWG; Thinopyrum intermedium) and Mountain Rye (Mtn Rye; Secale strictum) have potential for release as dual-purpose (grazing and grain production) perennial grains in Australia due to their superior longevity compared to hybrid perennial wheats. Initially developed as perennial forage grasses, few management guidelines exist to inform agronomic practices to maximise grain yields and profitability in Australian environments. An experiment was established in 2020 to examine the effect of plant density and nitrogen rates on grain yield components. The experiment compared the two species (IWG, Mtn Rye) sown at three plant densities (50, 100 and 200 plants/m2) …

Herding Livestock – The Phoenix Rises From The Ashes? Digital Herding As A Future Tool For Grazing Livestock, J. Horn, R. Reisch, M. Komainda, P. Riedel, J. Isselstein

IGC Proceedings (1977-2023)

Today, sustainable management of grazing livestock requires high efforts in management and fencing. Nowadays, several developments in digital technologies for herding grazing animals are arising. We conducted a systematic review on current developments in digital technologies for managing grazing animals within the landscape. We mainly focused on cattle (Bos taurus) and sheep (Ovis aries). We highlight the most promising developments of virtual fencing used in recent research to evaluate effectiveness, animal behaviour and welfare. Moreover, we highlight current research in digital herding by drones and robots. We discuss the potential and current limitations of digital tools …

Scaled Datasets Through Leveraging Technology Better Represent Plant Communities Classified Within National Land Cover Database (Nlcd), D. M. Johnston, D. B. Murray, Y. Y. Tsang

IGC Proceedings (1977-2023)

Technological advances in remote sensing have allowed for access to and application of large- scale datasets of plant communities using cover classification and chlorophyll content measurement with the use of multispectral sensors and vegetation indices. The United States’ primary remote sensing platform Landsat Thematic Mapper is a series of satellites providing 30-meter resolution imagery of the Earth’s surface. Efforts have been made to best utilize Landsat derivative National Land Cover Database (NLCD) datasets and provide spatial resolution commensurate with the needs of plant ecologists and range managers. However, usage of 30-meter spatial resolution imagery and indices does not allow detection …

Creating An Educational Experience That Fosters Diversity And Inclusion, M. M. Kohmann

IGC Proceedings (1977-2023)

Demographic, intellectual, and individual diversity have been linked to increase in innovation, problem-solving, critical thinking, and economic gains. However, there are multiple challenges associated with recruiting, retaining, and advancing underrepresented groups in Science, Technology, Engineering, and Mathematics (STEM). Studies have indicated negative implicit bias against women and minorities in terms of their perceived competence, worthiness to be hired, and payment irrespective to their qualification. Additionally, lack of mentoring and access to personal capital often hinder students’ academic advancement in STEM. However, there have been multiple intentional, recent efforts to increase and foster diversity in sciences. Some of the most effective …

Uf-Accelerate To Industry (A2i): Bringing Industry Into Academia, J. A. Estrada

IGC Proceedings (1977-2023)

University stakeholders are targeting candidates with competency in a wide range of professional skills, including team building and leadership, multi-modal communication, intercultural awareness, emotional intelligence, and mental flexibility. We developed UF-Accelerate to Industry (A2i) to deliver these skills to our graduate students and better prepare them to enter a competitive, rapidly evolving workforce. UF-A2i is delivered as day-long “immersion” events, consisting of presentations, panel discussions, skills workshops, and group projects. All activities are led by industry sponsors or career preparation specialists and are focused on building industry-identified skills that will give attendees a competitive edge in the job market. Since …

Ecosystem Services And Life Cycle Assessment Of Perennial And Annual Cropping Systems, M. Berti, A. Cecchin

IGC Proceedings (1977-2023)

Corn cultivation has negative impacts to the environment, such as nitrate leaching, soil erosion, and nitrous oxide emissions. Perennial crops such as alfalfa (Medicago sativa L.) reduce annual disturbance of soil, which affects many biogeochemical cycles that are key to provide resilience and stability to cropping systems. Four three-year crop sequences were evaluated. 1) Corn-soybean-corn (CSC); 2) alfalfa-alfalfa-alfalfa (AAA); 3) Corn-spring planted alfalfa-alfalfa (CAA); and 4) Corn-intercropped/alfalfa-alfalfa-alfalfa (CAIAA). Modeling of C and N cycles were conducted with the Denitrification-Decomposition (DNDC) model and global warming potential (GWP) was estimated using life cycle assessment (LCA) methodology. The soil organic carbon (SOC) balance …

Genetic Modification – Applications For Forage And Grassland Production Systems, J. R. Caradus

IGC Proceedings (1977-2023)

Genetic modification has been extensively used in crops for 25 years but has rarely been a technology used to advance trait expression in forage and grassland species. The exceptions are the use of herbicide tolerance and low lignin traits in alfalfa which are currently commercially available. In crop species genetic modification has been a valuable option for delivering improved economic and environmental outcomes through providing solutions for many of the challenges facing mankind. There are a many beneficial characteristics that genetic modification has the potential to provide in forage and grassland species. Managing these in the environment and understanding consumer …

Convalescing Soil Functionality Of Degraded Lands: An Experience From Semi-Arid India, A. Chandra, H. Baradwal, A. Ghosh, A. K. Singh, S. S. Manjangouda

IGC Proceedings (1977-2023)

Land loss is not only whittling away India’s gross domestic product by 2.5% every year and affecting its crop yield but also exacerbating climate change events in the country. To test applicability of alternate land use systems for improving soil functionality in restored ecologies, soils were sampled from the 0-15, 15-30 and 30-45 cm layers of Leucaena leucocephala (LL), Hardwickia binata (HB), Emblica officinalis (EO), Azadiracta indica (AI) based silviculture systems, Acacia nilotica-based silvipasture systems (AN), and natural grassland (NG) and compared with a fallow land (F). They were evaluated for carbon management index (CMI), nutrient supply capacity (NSC), …

The Impacts Of Collective Action In Common-Pool Resources ——Evidence From The Field Of Grassland Use, Wang Jizhe, Liu Min, Nie Zihan

IGC Proceedings (1977-2023)

The deterioration of common-pool resources is a serious issue faced by over the world now. How to manage public resources sustainably is extremely urgent. Collective action plays an important role in the sustainable management of common-pool resources. It creates a common goal that makes resource users not only concern their individual interests, but also the collective interests, in order to avoid the common-pool resource dilemma. The extent of collective interests that resource users concern affects their decisions in collective action, which further impacts the sustainable use of common-pool resources. We use a grazing experiment to observe and measure collective action. …

Physically Adjusted Ndf: Integrating Forage Physical Form With Diet Complexity On The Farm, P. J. Kononoff, M. B. Hall

IGC Proceedings (1977-2023)

Physical form of forages and their neutral detergent fiber (NDF) content are crucial in ruminant diets for maintaining a healthy rumen and a productive animal. A challenge has been in taking the concept from its research base and applying it successfully in varied diets. A new system for physically adjusted NDF (paNDF) offers an option. It takes into account the forage, NDF, starch and other components in the diet and uses the Penn State Particle Separator on farm to assess total mixed ration particle size to provide recommendations on directions to move the diet to maintain a healthy rumen. This …

Drought Tolerance Dissection And Molecular Breeding In Alfalfa, Luo Dong, Qiang Zhou, Xueming Dong, Wenxian Liu, Zhipeng Liu

IGC Proceedings (1977-2023)

Drought stress is one of the leading impediments that limit the productivity of global alfalfa (Medicago sativa). The underlying molecular and genetic mechanisms for drought tolerance in alfalfa remain largely unclear. In order to fully reveal the transcriptional changes of alfalfa in response to abiotic stress, the alfalfa transcriptome database under mannitol (simulated drought stress), NaCl (simulated salt stress), or exogenous ABA application was built via various RNA-seq technologies. Through further screening of the transcriptome database, a number of genes significantly induced by drought stress, such as the Nuclear Transport Factor 2-like (MsNTF2L), Drought-Induced Unknown Protein 1 (MsDIUP1), …

Grazing Behavior Of The Endemic Lagune Cattle In The Sub-Humid Savannahs Of Benin, E. Sonon, G. G. Alowanou, B. I. Koura, O. J. Makinde, A. B. Gbangboche

IGC Proceedings (1977-2023)

The indigenous Lagune, a breed tolerant to trypanosomiasis, is an excellent candidate to meet the ongoing environmental changes sustainably. This cattle breed could be kept like small ruminants, tethered in grasslands, or free grazing. A study was conducted to assess the grazing behavior of Lagune cattle under the two grazing systems to identify the best systems for sustainable production and conservation of the breed. For this study, four Lagune cattle farms, 02 under free-roaming and 02 others in the tethered system, were selected in the original belt of the Lagune cattle, the agro-ecological (AEZ) zones of Valley and Pobe. The …

Response Of Pasture Plants To Shade In Agroforestry And Agrivoltaic Production Systems, H. Kirschten, M. Graham, S. Ates, T. M. R. Maxwell, A. Rosati

IGC Proceedings (1977-2023)

Agrivoltaics production systems have gained traction globally, holding promise to supply energy and food concurrently from the same unit of land. Studies investigating shade tolerant crops to optimize production under solar panels have mostly followed the findings of agroforestry studies. However, spectral composition of transmitted radiation in agrivoltaic and agroforestry production systems may not be the same, inducing different shade responses of understory crops. A pot trial investigating the effect of shade source on biomass production and morphology of perennial ryegrass, ribwort plantain and white clover was established during spring, in Oregon, USA. Treatments were solar panel shade, hazelnut tree …

Simple Sars-Cov-2 Concentration Methods For Wastewater Surveillance In Low Resource Settings, Mohammad Dehghan Banadaki, Soroosh Torabi, Alexus Lori Rockward, William Strike, Ann Noble, James W. Keck, Scott M. Berry

UK CARES Faculty Publications

Wastewater-based epidemiology (WBE) measures pathogens in wastewater to monitor infectious disease prevalence in communities. Due to the high dilution of pathogens in sewage, a concentration method is often required to achieve reliable biomarker signals. However, most of the current concentration methods rely on expensive equipment and labor-intensive processes, which limits the application of WBE in low-resource settings. Here, we compared the performance of four inexpensive and simple concentration methods to detect SARS-CoV-2 in wastewater samples: Solid Fraction, Porcine Gastric Mucin-conjugated Magnetic Beads, Calcium Flocculation- Citrate Dissolution (CFCD), and Nanotrap® Magnetic Beads (NMBs). The NMBs and CFCD methods yielded the highest …

Screen-Printed Sensors Modified With Nafion And Mesoporous Carbon For Electrochemical Detection Of Lead In Blood, Elena Boselli, Zhizhen Wu, Erin N. Haynes, Ian Papautsky

UK CARES Faculty Publications

Lead (Pb) has long been acknowledged as a systemic toxicant, with pronounced health impacts observed even at low exposure levels, particularly in children. Adverse effects include diminished cognitive function, altered behavior, and developmental delays. Consequently, it is imperative to conduct regular monitoring of Blood Lead Levels (BLLs). In this work, we report on an electrochemical sensor based on screen-printed carbon electrode (SPCE) coated with Nafion and mesoporous carbon (MC). The sensor system uses simple sample preparation (acidification and dilution of whole blood), minimal sample volume (a few blood drops, 200 μl), and swift time-to-results (1 h). A limit of quantitation …