Digital Commons Network^™

Public University Gift Management Policies: Building A Best Practice Guide, Cristin Costello

MPA/MPP/MPFM Capstone Projects

Currently, no consistent standards exist for how institutions of higher education should manage their non-endowed gift revenue. Good stewardship of gift funds is vital to ensuring the maintenance of donor relationships to create unions that bring further financial opportunities to universities for years to come. Public institutions, as recipients of government dollars, are especially vulnerable to the negative repercussions that could come with the scandal of misappropriated gift dollars. Overall cash donations to higher education have significantly increased in the past few decades as state appropriations fall short and the need to keep tuition rates stable increases. It is more …

Reforming Need: How Certificate Of Need Laws In North Carolina Can Be Reformed To Promote Rural To Regional Health Network Integration, Taylor Fulk

MPA/MPP/MPFM Capstone Projects

Across North Carolina, many rural hospitals are facing closures, staffing shortages, and financial turmoil. Many hospitals, such as Morehead Hospital in Eden, NC (now UNC Rockingham), have utilized mergers with larger regional networks (e.g., UNC Health, Duke, Novant, Atrium) to stabilize operations and preserve local access to care. (deBruyn, 2017). However, even after successful integration efforts to bring some rural hospitals into larger regional networks, Certificate of Need laws in North Carolina are preventing these smaller, rural integrated hospitals from expanding their service offerings, thereby driving up healthcare expenditures for residents. As a result, patients experience longer-than-normal travel times and …

Unveiling Secrecy: A Qualitative Analysis Of The Breonna Taylor Grand Jury Proceedings, Taylor Bettis

Theses and Dissertations--Psychology

Grand juries have faced heightened scrutiny in recent decades following high-profile cases involving police use of lethal force against Black civilians. In 2020, Police officers killed Breonna Taylor while executing a search warrant at her home. Despite widespread public outcry, it remains unclear how prosecutors failed to indict the officers involved on charges directly related to her death. Guided by critical race theory, procedural justice, and theories of authority, the current project used reflexive thematic analysis to examine the Breonna Taylor grand jury proceedings. We identified two main themes: structural influences and narrative influences. Structural influences examine how prosecutorial authority, …

Distinct Odorant Receptor Response Patterns To Aliphatic Odorants In Freely Behaving Mice, Claire A. De March, Patrick Breheny, William B. Titlow, Hiroaki Matsunami, Timothy S. Mcclintock

Physiology Faculty Publications

In mammals, odors are encoded by a combinatorial code determined by the pattern of responses across hundreds of odorant receptors expressed monogenically and monoallelically in olfactory sensory neurons. The compositions of these receptor response patterns are largely unknown and overlap between them has yet to be explored. Activity-dependent reporter gene expression in freely behaving S100a5-tauGFP mice allowed capture of activated olfactory sensory neurons and identified 168 receptors responsive to moderate concentrations of 1 or more of 12 aliphatic (5 to 8 carbons) ketones, alcohols, and carboxylic acids. These 12 response patterns are remarkably different, with only 19% of the receptors …

Feeding- And Light-Cycle Synergistically Regulate Mouse Blood Pressure Daily Rhythm Via Bmal1-Dependent And Independent Mechanisms, Tianfei Hou, Wen Su, Aaron Chacon, An-Hsuan Lin, Zhenheng Guo, Ming C. Gong

Physiology Faculty Publications

Cardiovascular health requires the orchestration of the daily rhythm of blood pressure (BP), which responds to changes in light exposure and dietary patterns. Whether rhythmic light and feeding can modulate daily BP rhythm directly or via modulating intrinsic core clock gene Bmal1 is unknown. Using inducible global Bmal1 knockout mice (iBmal1KO), we explored the impact of rhythmic light, rhythmic feeding, or their combination on various physiological parameters. Daily rhythms of BP, heart rate, and locomotor activity were monitored via radiotelemetry, while food intake patterns were tracked using the BioDAQ system. Respiratory exchange ratio (RER) and energy expenditure (EE) were assessed …

S6k1 Is A Targetable Vulnerability In Tumors Exhibiting Plasticity And Therapy Resistance, Saptadwipa Ganguly, Ravshan Burikhanov, Vitaliy M. Sviripa, Sally Ellingson, Jieyun Jiang, Christian M. Gosser, David Orren, Eva M. Goellner, Gautham G. Shenoy, Mahadev Rao, John D’Orazio, Christine F. Brainson, Chang Guo Zhan, Peter H. Spielmann, David S. Watt, Vivek M. Rangnekar

Physiology Faculty Publications

Background: Most tumors initially respond to treatment, yet refractory clones subsequently develop owing to resistance mechanisms associated with cancer cell plasticity and heterogeneity. Methods: We used a chemical biology approach to identify protein targets in cancer cells exhibiting diverse driver mutations and representing models of tumor lineage plasticity and therapy resistance. An unbiased screen of a drug library was performed against cancer cells followed by synthesis of chemical analogs of the most effective drug. The cancer subtype target range of the leading drug was determined by PRISM analysis of over 900 cancer cell lines at the Broad Institute, MA. RNA-sequencing …

Sex-Based Differences In The Long-Term Fate Of Hippocampal Neurons Born After A Traumatic Brain Injury, Hannah C. Downing, Ashley B. Glover, Jessica E. Gebhardt, Katherine L. Thompson, Kathryn E. Saatman

Physiology Faculty Publications

Introduction: Moderate-to-severe traumatic brain injury (TBI) results in an early loss of immature hippocampal granule cells and the activation of typically quiescent neural stem cells (NSCs) in the dentate gyrus. Activation of NSCs leads to a robust increase in proliferation and generation of neural progenitor cells (NPCs), supporting restoration of the immature neuron population of over a period of 1–2 weeks. However, it is unclear if neurons born early after injury develop normally, survive long-term and functionally integrate into the hippocampal network. Although adult hippocampal neurogenesis is regulated in a sex-dependent manner, the majority of pre-clinical TBI studies lack the …

Skeletal Muscle Stem Cell Mitochondria Are Transferred To Muscle Fibers In Response To A Hypertrophic Stimulus, Jensen Goh, Julia G. Williams, Sarah E. Ogle, Jai K. Joshi, Logan N. Scott, Benjamin Burke, Alex R. Keeble, Nicholas T. Thomas, Christopher S. Fry, Ahmed Ismaeel, John J. Mccarthy

Physiology Faculty Publications

The fusion of skeletal muscle stem cell (MuSC) to myofibers during hypertrophy has exclusively focused on the transfer of the MuSC nucleus, leaving the fate of other MuSC organelles, such as mitochondria, largely unexplored. The objective of this study was to determine whether MuSCs transfer their mitochondria upon myofiber fusion in response to a hypertrophic stimulus. To achieve this goal, we specifically labeled MuSC mitochondria with Dendra2 fluorescence by crossing the MuSC-specific CreER (Pax7^CreER/CreER) mouse with the Rosa26-Dendra2 mouse to generate the Pax7-Dendra2 mouse. To induce the fusion of MuSC to myofibers, Pax7-Dendra2 mice underwent synergist ablation surgery …

Social Control And Inclusive Freedom: An Embedded Single Case Study Of Campus Expression At A Southern Public University, Christopher J. Ciarcia

Theses and Dissertations--Educational Policy Studies and Evaluation

This study examines how staff at a Southern public university experienced and interpreted their participation in the institution’s Open Expression Support Team (OEST), a campus initiative designed to maintain an educational environment where students feel free to engage in freedom of speech and expression without fear of intimidation or coercion. Using an embedded single-case study design, the research draws on interviews, documents, and observations to explore the OEST’s role in supporting inclusive freedom (Ben-Porath, 2017), which emphasizes the balance of free expression and inclusion in higher education.

Findings reveal that the OEST functions as a mechanism of social control, employing …

“Always Looking Over Your Shoulder”: College Students' Experiences With Codes Of Conduct At Evangelical Colleges & Universities, Kayla B. Powell

Theses and Dissertations--Educational Policy Studies and Evaluation

This qualitative research study investigated the experiences of college students enrolled at evangelical institutions that are affiliated with the Council for Christian Colleges & Universities (CCCU) and require students to follow an extensive code of conduct. These codes extend beyond academic expectations and mandate behavior in all aspects of daily life. Students who fail to comply are subject to a range of punishments as severe as expulsion. Sixteen traditional-aged undergraduate students from across the U.S. participated in semi-structured interviews that were analyzed using thematic analysis. Students described varied personal convictions regarding the rules; some earnestly strove to obey their campus …

Breaking Ground On Self-Efficacy: A Qualitative Exploration Of Intimate Partner Violence Survivors’ Experiences With Therapeutic Horticulture, Rachel Marie Reeves

Theses and Dissertations--Sociology

This dissertation explores how participation in a Therapeutic Horticulture program may support the self-efficacy of survivors of Intimate Partner Violence. Through a thematic analysis of interviews with 45 women living at a domestic violence shelter in Lexington, Kentucky, this study investigates whether and how gardening activities contributed to participants’ belief in their abilities to shape outcomes in their lives.

Findings are organized around Bandura’s four sources of self-efficacy: enactive mastery experiences, vicarious experience, verbal persuasion, and physiological/affective states. These insights contribute to feminist criminology and trauma-informed care literature by illuminating the transformative potential of nature-assisted healing programs.

The Role Of Metabolic Gate Keepers In Cell Fate Decision Making, Brian Kelly

Theses and Dissertations--Biology

Maintaining cellular energy balance and redox stability is crucial to cell survival, with glutamine metabolism playing a central role. While extensively studied in cancer, where it supports biosynthesis and reduces oxidative stress to promote tumor growth, its function in non-cancerous contexts like tissue development and muscle regeneration remains less understood. A key aspect of stem cell biology is quiescence, a metabolically dormant state that preserves the stem cell pool, yet the mechanisms regulating this state are not fully clear. Here, we identify glutamine metabolism as a critical factor governing myoblast proliferation and quiescence through a metabolic program distinct from that …

Heme Oxygenase, Biliverdin Reductase, And Bilirubin Pathways Regulate Oxidative Stress And Insulin Resistance: A Focus On Diabetes And Therapeutics, Wang-Hsin Lee, Zachary A. Kipp, Sally Pauss, Genesee J. Martinez, Evelyn A. Bates, Olufunto O. Badmus, David E. Stec, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Metabolic and insulin-resistant diseases, such as type 2 diabetes mellitus (T2DM), have become major health issues worldwide. The prevalence of insulin resistance in the general population ranges from 15.5% to 44.6%. Shockingly, the global T2DM population is anticipated to double by 2050 compared with 2021. Prior studies indicate that oxidative stress and inflammation are instrumental in causing insulin resistance and instigating metabolic diseases. Numerous methods and drugs have been designed to combat insulin resistance, including metformin, thiazolidinediones (TZDs), sodium-glucose cotransporter 2 inhibitors (SGLT2i), glucagon-like peptide 1 receptor agonists (GLP1RA), and dipeptidyl peptidase 4 inhibitors (DPP4i). Bilirubin is an antioxidant with …

Biparental Inheritance Of Germline-Specific Chromosomes In The Sea Lamprey And Their Roles In Oocytes, Vladimir A. Timoshevskiy, Nataliya Timoshevskaya, Kaan İ. Eşkut, Kasturi Rajandran, Jeramiah J. Smith

Markey Cancer Center Faculty Publications

Many eukaryotic species undergo programmed elimination of specific chromosomes during embryogenesis, typically retaining these chromosomes only in their germ cells. In some species, programmatic elimination of GRCs, or sex chromosomes, also occurs in a sex-specific manner, with specific chromosomes being transmitted or eliminated by only one sex. As such, these chromosomes provide a unique perspective on the evolution of gene functions that are advantageous to the germline and genetic tradeoffs between somatic vs germline or oocyte vs sperm biology. While GRCs have been extensively characterized in male sea lampreys (Petromyzon marinus), the status of GRCs in females has not yet …

Scalable Hypergraph Structure Learning With Diverse Smoothness Priors, Benjamin T. Brown

Theses and Dissertations--Electrical and Computer Engineering

In graph signal processing, learning weighted connections between nodes from signals is a fundamental task when the underlying relationships are unknown. With the extension of graphs to hypergraphs, where edges can connect more than two nodes, graph learning methods have similarly been generalized to hypergraphs. However, the absence of a unified framework for calculating total variation has led to divergent definitions of smoothness and, consequently, differing approaches to hyperedge recovery. This challenge is confronted in this work through generalization of several previously proposed hypergraph total variations, allowing ease of substitution into a vector-based optimization. To this end, a novel hypergraph …

Multi-Objective Design Optimization Of Power Converters For Electric Aircraft Propulsion, Ben Luckett

Theses and Dissertations--Electrical and Computer Engineering

As global focus shifts to the electrification of the aviation sector, the need for high efficiency, lightweight, and reliable electric aircraft propulsion power converter systems has become apparent. These goals can be somewhat conflicting with each other, and a single multi-domain-optimized solution is not guaranteed. The search for a design which presents satisfactory merits becomes a drudge through various trade-off studies which can expend vast quantities of manpower and time. As a remedy to this, design automation allows the process to be computer-assisted. This dissertation presents the core fundamentals for a general multi-objective design optimization framework intended for the design …

Influence Of Industrial Hemp On Soil Health Parameters In A Kentucky Cropping System, Jack T. Eaker

Theses and Dissertations--Plant and Soil Sciences

Industrial hemp (fiber, floral, and grain: Cannabis sativa L.) legalization has prompted research into its incorporation into agronomic rotations and impacts on agroecosystem services such as nutrient dynamics. This study quantified the effects of integrating grain and fiber hemp on soil health, hypothesizing hemp would positively impact soil health due to its taproot, reported C benefits, and nutrient requirements. Conducted in Kentucky at two locations, a completely randomized block design included six crop rotation treatments, with soil cores collected at initiation (2020) and post three growing seasons (2023). Soil parameters analyzed included particulate organic matter, short-term CO2 mineralization, water-extractable organic …

Revolutionizing Grapevine Breeding Programs: A Mobile Genome Editing System For Perennial Crops, Bridget Bolt

Theses and Dissertations--Plant and Soil Sciences

Grapevine (Vitis vinifera) faces unprecedented challenges from climate change, with up to 90% of current growing regions potentially becoming unsuitable for production within decades. Traditional breeding approaches require 25-30 years for variety development, creating a time constraint incompatible with the pace of environmental change. This research establishes the approach for a novel mobile RNA-based gene editing platform that addresses longstanding bottlenecks in grapevine improvement while preserving cultivar identity and reducing breeding timelines.

This research developed a three-component system for transgene-free gene editing in grapevine. First, a mobile CRISPR/Cas9 system was established using Agrobacterium rhizogenes transformation and the cut-and-dip …

Spanish-Speaking Interpreters’ Perspectives: How Latinx Immigrant Caregivers Navigate The Special Education Process, Lizeth B. Tomas Flores

Theses and Dissertations--Educational, School, and Counseling Psychology

The study examined the experiences of Spanish-speaking interpreters working with Latinx immigrant caregivers during the special education process, their perspectives on how caregivers and the IEP professional committee members participate in the process, aiming to gain a better understanding of how Latinx immigrant families navigate the special education process. Interpreters play a crucial role in the special education process for families from culturally and linguistically diverse backgrounds, as they facilitate the transmission of information between caregivers and special education teams. Interviews were conducted with six special education interpreters in a southwestern suburban school district in the United States. A qualitative …

“Hard To Rememeber, Then Hard To Forget:” A Qualitative Exploration Into The Attitudes And Lived Experiences Of Black People With Black Names, Destin L. Mizelle

Theses and Dissertations--Educational, School, and Counseling Psychology

Naming practices within the Black American community are vibrant and deeply rooted in cultural traditions and shared values. Much like other ethnic groups, these names tell stories, reflect heritage, and connect individuals to their community, making each choice meaningful and significant. However, due to the systemic effects of anti-Blackness, Black Americans often have a complex relationship with their names. While previous research has primarily utilized quantitative methodologies focusing on the negative associations and consequences of having a Black name, this study aims to amplify the voices of Black people who possess Black names. Using reflexive thematic analysis, this qualitative study …

The Impact Of Whiteness Among Black College Students' Racial Identity, La'quida Reneé Smith

Theses and Dissertations--Educational, School, and Counseling Psychology

Although the impact of racism is well-documented in both conceptual and empirical research on the experiences of Black college students, some scholars have pointed out that many of the negative, race-based experiences these students face on college campuses extend beyond overt acts of racism (e.g., individual racism, institutional racism; Gusa, 2010; Hargons et al., 2022; Ingram & Wallace, 2019; Le et al., 2020; Smith et al., 2011; Tyler et al., 2022). Whiteness has been identified as an overt factor shaping the lived experiences of Black individuals (Leonardo, 2009; Matias, 2016; Tyler et al., 2022) and is, therefore, believed to impact …

Pathways To Financial Wellness: Using Intersectionality Theory To Understand Black Women’S Financial Socialization, Courtney J. Wright

Theses and Dissertations--Educational, School, and Counseling Psychology

Black women experience high levels of financial vulnerability and consistently exhibit poor financial outcomes across the literature; however, few studies have investigated Black women’s financial socialization processes and outcomes. As a function of gendered racial marginalization, Black women occupy a unique standpoint that may influence their financial socialization experiences. Therefore, research that investigates Black women’s financial socialization and perceived financial capability, responsibility, and well-being is warranted.

Within this dual-manuscript style dissertation, the first manuscript investigates the sources, content, and methods of Black women’s family financial socialization messages. Using a critical-constructivist research paradigm and Reflexive Thematic Analysis for data analysis, findings …

Developing Novel Therapeutic Strategies For Mcrc With A Brafv600e Mutation, Mariah Geisen

Theses and Dissertations--Toxicology and Cancer Biology

Colorectal cancer (CRC) is the second leading cause of cancer death worldwide. The overall incidence rates of CRC have decreased in those over 50, but there is an increasing incidence rate in those under 50. Risk factors for CRC include diet, family history, and a sedentary lifestyle. Early detection of CRC is key to patient survival and treatment response. Localized CRC has a survival rate of 90% and allows for surgical resection and chemotherapy. The 5-year survival for metastatic CRC (mCRC) is significantly reduced to around 14%. Despite early detection, 50% of patients will develop metastasis after surgical resection and …

Investigating Plk1 In Pulmonary Fibrosis, Tempany Arbogast

Theses and Dissertations--Toxicology and Cancer Biology

Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease, with a median survival of only 2–3 years following diagnosis. In the United States, the disease contributes to more than 40,000 deaths annually, underscoring its severe clinical burden. Alveolar type II (AT2) lung epithelial cells serve as progenitors that maintain alveolar integrity by replenishing epithelial populations and producing surfactants, while fibroblasts and myofibroblasts regulate the extracellular matrix and structural support. In pulmonary fibrosis (PF) injury to AT2 cells promotes cytokine and growth factor release, driving inflammation, fibroblast proliferation, myofibroblast accumulation, and excessive extracellular matrix deposition. Aberrant AT2 cell …

Chemoproteomic Profiling Of A Carbon-Stabilized Gold(Iii) Macrocycle Reveals Cellular Engagement With Hmox2, Sailajah Gukathasan, Chibuzor Ngozi Olelewe, Libby Ratliff, Jong H. Kim, Alyson M. Ackerman, J. Robert Mccorkle, Sean Parkin, Gunnar F. Kwakye, Jill M. Kolesar, Samuel G. Awuah

Chemistry Faculty Publications

In this work, we discovered a novel organometallic gold(III) macrocycle, Au-Mac1, that demonstrates anticancer potency in a panel of triple-negative breast cancer cells (TNBC), and based on this complex, a biotinylated-Au-Mac1 probe was designed for target identification via chemoproteomics, which uncovered the engagement of HMOX2 of the heme-energy metabolism pathway. Using orthogonal chemical biology and molecular biology approaches, including immunoblotting, flow cytometry, and cellular thermal shift assays, it was confirmed that Au-Mac1 engages HMOX2 in cells. Downstream effects of Au-Mac1 on the depletion of mitochondrial membrane proteins and bioenergetics point to the potential role of HMOX2 in cancer. Importantly, Au-Mac1 …

Electrochemical Observation And Ph Dependence Of All Three Expected Redox Couples In An Extremophilic Bifurcating Electron Transfer Flavoprotein With Fused Subunits, Debarati Das, Wassim El Housseini, Monica Brachi, Shelley D. Minteer, Anne-Frances Miller

Chemistry Faculty Publications

Bifurcating enzymes employ energy from a favorable electron transfer to drive unfavorable transfer of a second electron, thereby generating a more reactive product. They are therefore highly desirable in catalytic systems, for example, to drive challenging reactions such as nitrogen fixation. While most bifurcating enzymes contain air-sensitive metal centers, bifurcating electron transfer flavoproteins (bETFs) employ flavins. However, they have not been successfully deployed on electrodes. Herein, we demonstrate immobilization and expected thermodynamic reactivity of a bETF from a hyperthermophilic archaeon, Sulfolobus acidocaldarius (SaETF). SaETF differs from previously biochemically characterized bETFs in being a single protein, representing a …

Characterization Of The Solid-State Bonding For Simulated Charge And Seam Welds In The Porthole Die Extrusion Process, Randall L. Bowers

Theses and Dissertations--Chemical and Materials Engineering

The isothermal (ISO-T) compression configuration is ideal for utilizing as a material input of flow stress at temperature and strain rate in models of the porthole die extrusion process due to the isothermal nature of the test. Micro-tensile testing was conducted to validate the solid-state welding integrity of the ISO-T samples at various temperatures and strain rates. Results of the mechanical testing were correlated with fractography and microstructural evaluations of the welds to confirm bonding behavior. Both clean interface conditions and oxidation and lubrication layers were evaluated to simulate the difference of seam and charge welds in the process. Results …

Combinatorial Thin Film Approach To Accelerate Materials Discovery: Development Of Nanoporous And Bulk Multi-Principal Element Alloys, Tibra Das Gupta

Theses and Dissertations--Chemical and Materials Engineering

Multi-principal element alloys (MPEAs) have garnered significant attention in materials science due to their potential for exhibiting a combination of desirable properties stemming from their unique configurational entropy. While early research focused on equiatomic compositions, recent studies have indicated that non-equiatomic MPEAs may offer superior mechanical performance. However, the identification of optimal non-equiatomic compositions remains challenging due to the vast compositional space and the reliance on time-consuming and computationally expensive methods. This dissertation presents a combinatorial thin film approach as a rapid and efficient strategy for discovering two types of materials: nanoporous and bulk MPEAs.

The first study explores the …

Chromatographic And Electrophoretic Analysis Of Lignin-Derived Monomers And Dimers: Extraction Method Development And Evaluation Of Cyclodextrins For Recovery, Anjam Sadik

Theses and Dissertations--Chemistry

Biorefineries play a vital role in reducing dependence on fossil fuels and addressing global warming concerns. Unfortunately, investment in biorefineries remains limited due to economic sustainability concerns. One approach to enhance the economic viability of biorefineries is by fully utilizing the potential of feedstocks, particularly lignocellulosic biomass, which is commonly used in these processes. Traditional processing methods often alter the lignin portion of biomass, making it unsuitable for further utilization. However, when retained in its native form, lignin can serve as a valuable source of chemicals that contribute to the economic sustainability of biorefineries.

The lignin-first approach, which removes lignin …

Catalytic Deoxygenation Of Oleaginous Biomass To Renewable Diesel And Sustainable Aviation Fuel Via Decarboxylation/Decarbonylation, Chukwudalu Great Umenweke

Theses and Dissertations--Chemistry

Several technologies have been developed to produce hydrocarbon biofuels – renewable diesel (RD) and sustainable aviation fuel (SAF) – from fats, oils, and greases (FOG), with the hydroprocessing of esters and fatty acids (HEFA) representing one of the most mature pathways. In its current form, HEFA is mainly reliant on the hydrodeoxygenation (HDO) reaction, which has several drawbacks since HDO requires large amounts and pressures of hydrogen, feedstocks of high purity and cost, as well as problematic sulfided catalysts that risk contaminating the biofuel product with sulfur. A process based on decarboxylation/decarbonylation (deCO_x) offers an attractive alternative to …