Digital Commons Network^™

Maturation Of Equine Proximal Sesamoid Bones In Thoroughbred Horses, Angela Maria Mangine

Theses and Dissertations--Veterinary Science

Proximal sesamoid bone (PSB) injuries account for approximately 50% of all fatal catastrophic musculoskeletal injuries in Thoroughbred racehorses. In addition, PSB fractures are not uncommon in Thoroughbred foals and yearlings as a result of energetic or intense free exercise. The ability to interpret some PSB pathology assessments, however, is more difficult given the limited information published on the normal development and maturation of these paired sesamoid bones. In addition, the level of normal variation in PSB morphological and structural parameters within the Thoroughbred population as a function of age, body size, gender, and inter-animal variation are not well documented, complicating …

Muscle Mass And Immune Function In The Senior Horse, Alisa Christina Herbst

Theses and Dissertations--Veterinary Science

Senior horses (≥ 15 years) represent up to one-third of the global equine population, and the proportion of old horses (≥ 20 years) in the U.S. has been steadily increasing. Aging is associated with a loss of skeletal muscle mass in horses, and while age-related muscle loss is comparingly well characterized in humans, little is currently known concerning underlying mechanisms, adverse outcomes, or the prevalence of low muscle mass in senior horses. One factor proposed to play a role in the development of age-related muscle atrophy in humans is inflamm-aging, a low-grade inflammation that affects elderly people and that has …

Vole Population Dynamics In Cover Crops Transitioning To Soybeans With Integrated Pest Management By Habitat Modification, Jena L. Nierman

Theses and Dissertations--Forestry and Natural Resources

The use of cover crops has been a wildly used method in rotational row crop production. Cover crops have minimized soil runoff and aided in maintaining nutrients in agricultural fields. Increased use of cover crops has seen a corresponding increase in the amount of damage done to soybeans by voles. Currently, there are no mitigation methods that successfully decrease vole populations in agricultural fields. The use of habitat manipulation as an integrated pest management solution has not been studied as a practical solution for vole population management. During 2019 and 2020, I tested the impacts of various cover crop termination …

Sustainability And Economics Of White Oak (Quercus Alba) Timber Supply In Kentucky, Gaurav Dhungel

Theses and Dissertations--Forestry and Natural Resources

Sustained timber supply relies upon balanced forest age distribution, where the level of harvesting and mortality is compensated by regeneration and growth among younger age classes. However, current forest inventory trends from the white oak growing region reveal sustainability threat from declining white oak regeneration and recruitment leading to disproportionate inventory structure. Consequently, there exists growing concern among stakeholders on long-term sustainability of white oak timber and its economic implications, particularly in Kentucky, because of the significance of the species to the state’s economy. This research aims to examine past and current inventory levels of white oak in Kentucky using …

The Impacts Of Imazapic On Garlic Mustard And Non-Target Forest Floor Vegetation In Central Kentucky’S Hardwood Forests, Pavan Kumar Podapati

Theses and Dissertations--Forestry and Natural Resources

Alliaria petiolata is an invasive biennial herb that poses a substantial threat to various ecosystems across the United States. Imazapic herbicide can control A. petiolata infestations, but there is limited peer-reviewed data on impacts of pre-emergent imazapic spraying to forest floor communities. This research examined the impacts of pre-emergent imazapic (0.84 kg/ha) with Pentra-Bark® surfactant on ground cover of A. petiolata and the spring perennials Claytonia virginica and Erigenia bulbosa. Experimental populations in randomized blocks within two forest stands in central Kentucky received the following treatments at 0.84 kg ai/ha: imazapic with Pentra-Bark®, glyphosate with Pentra-Bark®, Pentra-Bark® alone, and …

Quantifying The Source And Pathway Of Dissolved Reactive Phosphate In Karst Drainage Of The Inner-Bluegrass, Cory Radcliff

Theses and Dissertations--Biosystems and Agricultural Engineering

In the Midwestern U.S. seasonal hypoxia experienced in the Gulf of Mexico and harmful algal blooms in inland freshwater ponds, lakes, and rivers are partly fueled by dissolved orthophosphate loadings from disturbed landscapes. Efforts to reduce dissolved reactive phosphate (DRP) loadings have had varying levels of success and have led to insufficient water quality improvements. Inefficiencies in conservation strategies can stem from poor understanding of phosphate source and flow pathway dynamics. This study focused on monitoring sources and flow pathways of dissolved reactive P in a karst agroecosystem with phosphatic limestone. We collected event water samples at the Camden Creek …

Extraction Of Micro- And Nano-Plastic Particles From Water Using Hydrophobic Natural Deep Eutectic Solvents, Jameson R. Hunter

Theses and Dissertations--Biosystems and Agricultural Engineering

The production of plastic and the amount of waste plastic that enters the environment increases every year. Synthetic polymers will gradually break down into particles on the micro- and nano-scale. The micro- and nano-plastics pose a significant ecological harm by transporting toxic chemicals and causing inflammation and cellular damage when ingested. Two common plastics are polyethylene terephthalate (PET) and Polystyrene (PS), and a newer bioplastic polylactic acid (PLA) that has become a popular alternative. Deep eutectic solvents are a recently discovered solvent composed of a hydrogen bond donor and hydrogen bond acceptor and have been proposed as a cheaper alternative …

Characterization And Upgrade Of Endocarp Biomass Derived Lignin To Value Added Products, Makua Vin-Nnajiofor

Theses and Dissertations--Biosystems and Agricultural Engineering

Lignin is an abundant biopolymer and a promising source of feedstock for high-value chemicals and energy materials, however, application methods of lignin into valuable products is yet to be fully explored. This study explores the valorization of lignin derived from lignin-rich endocarp biomass through the development of lignin-derived electrode materials. The chemical composition and cellular structure of the endocarps were characterized using HPLC and scanning electron microscopy (SEM) imaging. Mechanical properties of walnut and peach endocarps and preprocessing requirements were investigated using nanoindentation and specific energy consumption during grinding. Next, lignin was extracted from endocarps using deep eutectic solvent and …

Evaluating The Biotic Condition Of Restored Streams In Kentucky’S Inner Bluegrass Region, Charles Cole Crankshaw

Theses and Dissertations--Biosystems and Agricultural Engineering

Numerous stream restoration projects have been implemented in Kentucky’s Inner Bluegrass region to offset anthropogenic impacts. These projects range from full channel realignments to volunteer-led riparian installations. To assess the ability of said projects to restore stream habitat and biota, full restoration (n=12) and riparian (n=6) sites were compared to reference (n=6) and disturbed (n=12) sites using RBP and SVAP protocols, macroinvertebrate samples, and geomorphology. General trends for SVAP, RBP, and BI scores, starting with highest habitat or biotic quality, were reference sites, full and riparian restoration sites, then disturbed sites. The number of EPT taxa, another indicator of biological …

Southern Pine Beetle-Specific Rna Interference Demonstrates No Effects On Nontarget Insects, Hannah Marie Hollowell

Theses and Dissertations--Entomology

The endemic southern pine beetle (SPB), Dendroctonus frontalis Zimmermann, is a highly destructive bark beetle that has devastated coniferous forests of the southern United States for decades. Traditional management strategies have been unable to respond effectively to largescale SPB outbreaks, prompting exploration of novel techniques including RNA interference (RNAi). Double-stranded RNAs (dsRNAs) are effective at triggering gene silencing and inducing mortality in SPB, but determining delivery methods and demonstrating specificity of the treatments is necessary before this technology may progress to deployment.

I evaluated three SPB-specific dsRNAs on model nontarget insects to assess potential lethal effects, sublethal effects such as …

Distribution And Diversity Of Heliothine And Other Lepidopteran Nudiviruses, Emrah Ozel

Theses and Dissertations--Entomology

Helicoverpa zea nudivirus 2 (HzNV-2) is the only known sterilizing and sexually-transmitted insect virus and causes pathological symptoms in H. zea reproductive tissues. HzNV-2 has features that make it a candidate as a H. zea (corn earworm) control agent, such as the ability to cause asymptomatic (latent) and symptomatic (lytic) infections and the ability to influence mating behavior of its host to favor virus spread. HzNV pathology has been studied and its genome sequenced, however, its prevalence in natural populations is largely unknown. In this study, we developed and used a low-cost PCR-based molecular survey to investigate HzNV-2 prevalence and …

A Qualitative Examination Of The Agency Of Women In Their 30s And 40s Who Use Dating Applications, Tera Buerkle

Theses and Dissertations--Family Sciences

The use of dating applications (apps) to find romantic and sexual partners is widespread across age groups, however, there is a paucity of research on dating apps with those in middle adulthood. Sexual script theory suggests that women’s agency (i.e. the ability to act in one’s own best interest) may be impacted by expectations from an inherently sexualized context, such as dating apps. Feminist theory contends that women’s agency is complicated by gender socialization due to the imbalance of power in society that greatly favors men. In this study seventeen women aged 30 to 49 completed in-depth semi-structured interviews, and …

Assessing Self-Perceived Nutrition-Related Lifestyle Behaviors And Knowledge Of University Music Students Following A Targeted Presentation, Gretchen Trumbo

Theses and Dissertations--Nutrition and Food Systems

Although proven that nutrition can impact physical health, limited research examines the relationship between nutrition-related lifestyle behaviors, nutrition knowledge, and the prevalence of injury in university music students. The objective of this study is to evaluate nutrition-related lifestyle behaviors and self-perceived nutrition knowledge of university music students before and after a targeted nutrition education session. A baseline Qualtrics survey was administered to undergraduate and graduate music students (18+ years) via the University of Kentucky School of Music Listserv to gather data related to demographics, anthropometrics, history of playing-related injury, nutrition knowledge, attitudes, beliefs, and behaviors. A nutrition education presentation, focused …

Psychological Distress And Relationship Satisfaction Among Survivors Of Sexual Violence, Alyssa Campbell

Theses and Dissertations--Family Sciences

The World Health Organization (WHO; 2002) has indicated that sexual violence is a serious public health concern, and both the WHO and the United Nations (UN) have declared that violence against women, in particular, is a profound violation of human rights (UN General Assembly, 1993; WHO, 2017). Although the systemic and negative impact of trauma on family and intimate relationships have been well documented, the empirical literature regarding the effects of adult sexual trauma on relationship satisfaction is less robust. These studies are designed to address this gap and will do so with analyses centered on an understudied population: the …

Birth Mothers’ Experiences Of Support Before, During, And After Adoptive Placement, Ciara Watkins

Theses and Dissertations--Family Sciences

The current study explores the perceptions and experiences of support in a sample of birth mothers who relinquished parental rights though open or closed private adoption (e.g., religiously, and non-religiously affiliated private adoption agencies, adoption attorneys). Participants (N = 51) were birth mothers who placed a child for adoption at birth or within several months and who relinquished parenting rights 8 months to 50 years ago, with (M = 15.39) years since placement. A thematic analysis uncovered six overarching themes throughout the relinquishment process. Further, prevalence of themes in certain phases (i.e., pre-placement, during placement, post-placement) were shared among all …

The Evaluation Of An Amino Acid- Oral Re-Hydration Solution (Enterade®) To Improve Quality Of Life In Solid Tumor Cancer Patients By Reduction Of Chemotherapy Related Diarrhea, Holly Chitwood

DNP Projects

Background: Chemotherapy related diarrhea (CRD) is a common adverse effect of many chemotherapy agents used to treat cancer. CRD negatively affects quality of life (QOL) for cancer patients and often results in treatment delays, hold, dose reductions, and hospitalizations affecting morbidity and mortality. Proper management of CRD with novel therapeutics is needed to improve the quality of life and patient outcomes for this population.

Purpose: The purpose of this study was to evaluate the use of a proprietary amino acid-oral rehydration solution (AA-ORS) known as Enterade® to reduce the severity of CRD, to improve patient reported QOL among patients over …

Secular Trends In Low Birth Weight And Preterm Birth Disparity Between Infants Born To Teenage And Adult Mothers: An Exploratory Analysis, Elizabeth Edwards

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Introduction. Increased risk of preterm birth and low birthweight for infants born to teenage mothers (15-19), when compared to infants born to adult mothers (20-29), is well documented in the United States. Trends in preterm birth and low birthweight are also well documented over time. Examination of the difference in incidence of preterm birth or low birthweight between infants born to teenage mothers and to adult mothers over time is not present in the literature. This study aimed to fill this gap in the literature and determine how preterm birth and low birthweight differences have changed over time.

Methods. Natality …

Colorectal Cancer Differences In Staging And Survival: Appalachian And Non-Appalchian Kentuckians, Ripley Lucas

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Colorectal cancer is the third most commonly diagnosed cancer in the United States. Historically, the Appalachian region of Kentucky has experienced disparities in colorectal cancer screening and survival as compared to both the non-Appalachian region of the state and the United States. The aim of this paper is to investigate the differences in cancer staging and survival after diagnosis in Appalachian and non-Appalachian Kentuckians. The present time period of study is a retrospective, population-based cohort with data taken from the Kentucky Cancer Registry. Binomial logistic regression and Cox proportional hazards models were used to estimate unadjusted and adjusted odds ratios …

Accumbens Cholinergic Interneurons Mediate Cue-Induced Nicotine Seeking And Associated Glutamatergic Plasticity, Jonna M. Leyrer-Jackson, Michael Holter, Paula F. Overby, Jason M. Newbern, Michael D. Scofield, M. Foster Olive, Cassandra D. Gipson

Family and Community Medicine Faculty Publications

Nicotine, the primary addictive substance in tobacco, is widely abused. Relapse to cues associated with nicotine results in increased glutamate release within nucleus accumbens core (NAcore), modifying synaptic plasticity of medium spiny neurons (MSNs), which contributes to reinstatement of nicotine seeking. However, the role of cholinergic interneurons (ChIs) within the NAcore in mediating these neurobehavioral processes is unknown. ChIs represent less than 1% of the accumbens neuronal population and are activated during drug seeking and reward-predicting events. Thus, we hypothesized that ChIs may play a significant role in mediating glutamatergic plasticity that underlies nicotine-seeking behavior. Using chemogenetics in transgenic rats …

Race Youth In Twentieth-Century American Literature And Culture, Claire E. Lenviel

Theses and Dissertations--English

Race Youth in Twentieth-Century American Literature and Culture argues for the centrality of black youth, both real and literary, to the trajectories of African American literature and its repudiation of white supremacy. Drawing on research into the rise of the adolescent and teenager as distinct social categories, I argue that age-based subjectivity should inform how we read race-based subjectivity. My first chapter explores how early twentieth-century black periodicals push back against white supremacist theories of human development in an explicit appeal to what I call “race youth,” the children and adolescents who would take up the mantle of racial uplift. …

Novel Computational Methods For Cancer Genomics Data Analysis, Jinpeng Liu

Theses and Dissertations--Computer Science

Cancer is a genetic disease responsible for one in eight deaths worldwide. The advancement of next-generation sequencing (NGS) technology has revolutionized the cancer research, allowing comprehensively profiling the cancer genome at great resolution. Large-scale cancer genomics research has sparked the needs for efficient and accurate Bioinformatics methods to analyze the data. The research presented in this dissertation focuses on three areas in cancer genomics: cancer somatic mutation detection; cancer driver genes identification and transcriptome profiling on single-cell level.

NGS data analysis involves a series of complicated data transformation that convert raw sequencing data to the information that is interpretable by …

Biocultural Evidence Of Precise Manual Activities In An Early Holocene Individual Of The High-Altitude Peruvian Andes, Fotios Alexandros Karakostis, Hugo Reyes-Centeno, Michael Franken, Gerhard Hotz, Kurt Rademaker, Katerina Harvati

Anthropology Faculty Publications

OBJECTIVES: Cuncaicha, a rockshelter site in the southern Peruvian Andes, has yielded archaeological evidence for human occupation at high elevation (4,480 masl) during the Terminal Pleistocene (12,500–11,200 cal BP), Early Holocene (9,500–9,000 cal BP), and later periods. One of the excavated human burials (Feature 15‐06), corresponding to a middle‐aged female dated to ~8,500 cal BP, exhibits skeletal osteoarthritic lesions previously proposed to reflect habitual loading and specialized crafting labor. Three small tools found in association with this burial are hypothesized to be associated with precise manual dexterity.

MATERIALS AND METHODS: Here, we tested this functional hypothesis through the application of …

Innovative Statistical Models In Cancer Immunotherapy Trial Design, Jing Wei

Theses and Dissertations--Statistics

A challenge arising in cancer immunotherapy trial design is the presence of non-proportional hazards (NPH) patterns in survival curves. We considered three different NPH patterns caused by delayed treatment effect, cure rate and responder rate of treatment group in this dissertation. These three NPH patterns would violate the proportional hazard model assumption and ignoring any of them in an immunotherapy trial design will result in substantial loss of statistical power.

In this dissertation, four models to deal with NPH patterns are discussed. First, a piecewise proportional hazards model is proposed to incorporate delayed treatment effect into the trial design consideration. …

A Pilot Study Evaluating A Training For Music Therapy Students On Considerations For Lgbtq Clients, Cecilia Blair Wright

Theses and Dissertations--Music

Music therapists are expected to understand the influence of gender identity, gender expression, and sexual orientation on the therapeutic process, yet may not be receiving education that adequately prepares them to support LGBTQ clients. The purpose of this pilot study was to evaluate the impact of a one-hour educational training for music therapy students on considerations for working with LGBTQ clients. The presentation included information about terminology, discrimination, healthcare discrimination, music therapy research, musical considerations, and additional resources provided as a handout. The minority stress theory and queer theory informed the development of the presentation. Exploratory analysis revealed that participants …

Songs For High Voice: An Annotated Guide To African Romances, Op. 17 By Samuel Coleridge-Taylor, Makeda Danielle Hampton

Theses and Dissertations--Music

African Romances, Op. 17, composed in 1897 by African-British composer Samuel Coleridge-Taylor (1875-1912), is a collection of seven songs for high voice that is uniquely both African and American. The lyrics of this song cycle were first published in the book Majors and Minors, a collection of poems published in 1895 by Paul Laurence Dunbar (1872-1906).

An analysis of resources supports that academic discourse in Black vocal music has been underrepresented due to the absence of centralized information, such as published scores, recorded materials, catalogs, and guides for study and performance. While in depth research focusing on the art …

Shining A Spotlight On Female African American Composer Evelyn Simpson-Curenton, Iris E. Fordjour-Hankins

Theses and Dissertations--Music

Those who are interested in learning more about Evelyn Simpson-Curenton (b. 1953) will find it challenging to find scholarly material on her life and music. She is best known for her arrangements of spirituals that were sang and recorded by Kathleen Battle and Jessye Norman (whose concert recording Spirituals in Concert was released in 1991). Simpson-Curenton is also known for her choral work, “Psalm 91,” made famous by Oakwood University’s Aeolians. Though she is self-published, her absence from compilations, volumes, and anthologies for solo voice and piano is regrettable. This dissertation provides well researched scholarly information about her musical life …

The Evaluation Of The Role Of Research Administrators In The Biomedical Research Grant Lifecycle And How Public Universities’ Policies Are Designed To Protect Funding, Megan Eder

MPA/MPP/MPFM Capstone Projects

There was a time in the not-so-distant past, where a simple infection had the opportunity to send an individual to an early grave. With advancements in biomedical research, medical professionals can treat simple infections as well as more complicated illnesses. Medical research has come a long way, but there is still a need for research in order to find, treat, and advance cures for illness and disease. Last year, during the COVID-19 pandemic, the entire world was waiting for researchers to create a vaccine to help slow the spread and get the world back to operating at a normal capacity. …

The Characterization Of Purified Citrate-Coated Cerium Oxide Nanoparticles Prepared Via Hydrothermal Synthesis, Matthew L. Hancock, Robert A. Yokel, Matthew J. Beck, Julie L. Calahan, Travis W. Jarrells, Eric J. Munson, George A. Olaniyan, Eric A. Grulke

Chemical and Materials Engineering Faculty Publications

Hypothesis

Cerium oxide nanoparticles were synthesized using a hydrothermal approach with citric acid as a stabilizing agent. Citric acid adsorption onto the nanoceria particle surface can cease particle formation and create a stable dispersion for an extended shelf life. The product was dialyzed immediately following the synthesis to remove unreacted cerium that could contribute to biological effects. Nanoparticle characterization results are expected to help identify the surface citrate bonding structure.

Experiments

Many characterization techniques were utilized to determine size, morphology, surface properties, and citrate complexation on the nanoceria particle surface. These included transmission electron microscopy, electron energy loss spectroscopy, dynamic …

Periocular Mesenchyme Heterogeneity During Morphogenesis Of The Vertebrate Ocular Anterior Segment, Kristyn L. Van Der Meulen

Theses and Dissertations--Biology

The vertebrate eye is a complex organ, responsible for the primary sense with which we interact with our environment: vision. Development of the eye is a tightly regulated process, controlled by a vast network of genes. This process begins with eye morphogenesis, when the eye structure is formed through a series of morphogenetic movements and culminates in the creation of the optic cup, lens, and presumptive optic stalk. Next, retinal differentiation creates the critical cell layers of the retina needed to process light waves that enter the eye, including rod and cone photoreceptors, interneurons, and support cells. Failure in either …

Fluid Dynamics: Representations Of Water In Music, James E. Evans

Theses and Dissertations--Music

Water has remained a subject of all kinds of musical works since at least the middle ages. These musical works lack the concrete representational capacity of paintings, photographs, and films, relying instead on more abstract metaphorical constructs to convey water imagery. Current scholarship on water music typically centers on Romantic and Impressionist works and does not examine the process of signification by which musical signs portray water. The principal goal of this study is to determine how musical devices convey specific aspects of bodies of water and how such devices interact and contribute to musical depictions of streams, rivers, lakes, …