Digital Commons Network^™

Detection Of Morphine And Its Analogues Using Enzymatic Hydrolysis, Joan Combie, Jerry W. Blake, Thomas E. Nugent, Thomas Tobin

Veterinary Science Faculty Patents

The invention relates to a method for hydrolyzing drug glucuronic acid conjugates present in mammalian body fluids, the conjugates being derived from a narcotic analgesic, antagonist, or agonist-antagonist whose metabolism includes conjugation with glucuronic acid. The method comprises incubating the body fluid sample at from about 60 to about 70° C., for at least about 1 hour, with 3-glucuronidase derived from Patella vulgata, and substantially increases the sensitivity of chromatographic techniques for the detection of morphine and its analogues.

Jackson V. Virginia: Reopening The Pandora's Box Of The Legal Sufficiency Of Drug Identification Evidence, Edward J. Imwinkelried

Kentucky Law Journal

No abstract provided.

Activation Of B Lymphocytes By Monovalent Anti-Lyb-2 Antibodies, Subbarao Bondada, D. E. Mosier

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Small resting B lymphocytes are activated when their cell surface immuno-globulin (Ig) receptors interact with specific antigen or anti-Ig antibodies. Such a receptor-ligand interaction leads to enlargement and proliferation of B lymphocytes. These activated B cells can subsequently mature into Ig-secreting cells in the presence of appropriate accessory cell signals (1-3). However, monovalent anti-Ig antibodies cannot induce B cell activation even though they can bind to the surface Ig (4-7). It has thus been concluded that membrane Ig has to be cross-linked to induce early steps in B cell activation (membrane depolarization; 8) as well as later steps (proliferation). T …

Incest Statutes And The Fundamental Right Of Marriage: Is Oedipus Free To Marry?, Carolyn S. Bratt

Law Faculty Scholarly Articles

The U.S. Supreme Court has found that the right to marry is a constitutionally protected right. That right is restricted, however, by state incest statutes which impede marriage between adults by making some choices of a marriage partner illegal. The constitutional validity of modern state incest statutes is difficult to analyze because of shifting definitions, reflexive fears, ambivalent attitudes, and underlying facile generalizations.

The mere word "incest" triggers strong feelings of revulsion in most people. Therefore, any a priori labeling of a marriage as incestuous tends to preclude objective thought about the permissibility of the particular form of the marriage …

The Red River Gorge: The Existence Of "Recreational Niches" And Their Management Implications, Sara E. Alexander, Billie R. Dewalt, Eugenie Scott

KWRRI Research Reports

Until the late 1960's, the Red River Gorge was just one of the more beautiful parts of the Daniel Boone National Forest. At this time, the Army Corps of Engineers proposed to dam the river for flood control, water supply, and recreation purposes. Intense opposition to the project generated considerable publicity and the Gorge area experienced substantial increases in visitation. The result has been not only severe environmental degradation but extensive overcrowding as well. These trends are presently overtaxing management capabilities of the United States Forest Service. The most pressing issue has come to be how to effectively manage this …

Atlas Of Wetlands In The Principal Coal Surface Mining Region Of Western Kentucky, William J. Mitsch, Jan R. Taylor, Kimberly B. Benson, Paul L. Hill Jr.

KWRRI Research Reports

This atlas contains maps of wetlands and surface mining activity in the Western Kentucky Coal Field, and focuses on a 3960 km² (1530 mi²) region where approximately 90 percent of surface mining in the Coal field occures. Some present and potential competition exists between surface coal mining and wetland protection. A wetland classification, based on the recent FWS classification, includes six types of palustrine systems and one each of riverine and laucstrine systems. Wetlands and surface mines are located on twenty-seven 7.5 minute USGS quadrangles that define the study region. A total of 460 km² (177 …

Modeling Surface And Subsurface Stormflow On Steeply-Sloping Forested Watersheds, Patrick G. Sloan, Ian D. Moore, George B. Coltharp, Joseph D. Eigel

KWRRI Research Reports

A simple conceptual rainfall-runoff model, based on the variable source area concept, was developed for predicting runoff from small, steep-sloped, forested Appalachian watersheds. Tests of the model showed that the predicted and observed daily discharges were in good agreement. The results demonstrate the ability of the model to simulate the "flashy" hydrologic behavior of these watersheds.

Five subsurface flow models were evaluated by application to existing data measured at Coweeta on a reconstructed homogeneous forest soil. The five models were: Nieber 's 2-D and 1-D finite element models (based on Richards' equation), the kinematic wave equation, and two simple storage …

The Induction Of Tolerance To Heavy Metals In Natural And Laboratory Populations Of Fish, Wesley J. Birge, William H. Benson, Jeffrey A. Black

KWRRI Research Reports

Aquatic toxicity studies were performed on two natural populations of fathead minnows. One group of organisms was taken from a metal-contaminated flyash pond associated with a coal-fired power plant and the other group was collected from relatively uncontaminated hatchery ponds. Acute tests indicated that flyash pond fish were significantly more tolerant to cadmium and copper than were hatchery fish. At an exposure concentration of 6.0 mg Cd/L in moderately hard water, the median period of survival for flyash pond fish was 50.0 hr compared to 6.8 hr for hatchery fish. Both groups of organisms were about equally sensitive to zinc. …

College Of Law Commencement Program, 1983, University Of Kentucky

Graduation Programs

No abstract provided.

Kentucky's Ipm Program For Alfalfa, Chris M. Christensen

Kentucky Alfalfa and Stored Forage Conference

Integrated Pest Management (IPM) is a concept that encourages the use of a variety of strategies in the management of crop pests. It is very important to have an indepth understanding of the crop production system when implementing an IPM program. An IPM program is dynamic requiring a continual evaluation of the status of the crop, the associated pest complex, the effects of the pests and the biological deterrents to those pests. If a pest population gets "out of hand" and begins to cause economic damage, pest management techniques may need to be applied. These pest management measures may be …

Feticide: Murder In Kentucky?, Perry Mack Bentley

Kentucky Law Journal

No abstract provided.

Pharmacokinetics And Protein Binding Of Morphine In Horses, Joan Combie, Thomas E. Nugent, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Morphine could be detected in horses dosed with 0.1 mg of drug/kg of body weight for up to 48 hours in blood and 144 hours in urine. This dose of morphine elicited no observ­able effects and is a suggested an­algesic dose. Computer analysis revealed that a 3-compartment open system was the best fitting model with a serum half life of 87.9 minutes and a urine half life of 101.1 minutes. Binding to equine serum proteins was linear over a drug con­centration range of 3.88 x 10-5M to 3.50 x 10-aM and averaged 31.6%. In RBC-partitioning experiments, 78.1 % of the …

Fraud Between Sexual Partners Regarding The Use Of Contraceptives, Diane M. Carlton

Kentucky Law Journal

No abstract provided.

Does No-Till Change Soil Management Practices?, Robert L. Blevins

Soil Science News and Views

Successful no-tilling requires a different approach to soil management practices. Since continuous no-tillage systems leave residues on the soil surface without mechanically mixing them into the plow layer and since lime and fertilizer are surface-applied, no-tilled soils have biological, chemical and physical properties contrasting with those of a plowed soil. For any crop production system to be widely accepted and used it must provide and maintain desirable physical properties of the soil, control erosion and replace nutrients removed by crops and other losses. This can be accomplished in a no-till system if proper management is used.

Some Variations In Distribution Of Fishes In Large Mainstream Reservoirs Associated With Artificial Cover, Donald W. Johnson, Elizabeth M. Choinski

KWRRI Research Reports

The influence of artificial cover (brush piles) on fish populations in Kentucky Lake and Lake Barkley was studied. Mature and larval fishes were collected from deep and shallow sites with and without cover in a bay of each lake. Highest densities of mature crappie, bass, and sauger were found adjacent to deep attractors, while larval crappie and minnows were most concentrated at shallow brush piles. Shad (both adult and larvae) were not congregated at attractor sites. Information gathered supports the continuation of artificial cover installation and water level management procedures which will provide high and stable levels through spring spawning …

Landowners, Recreationists, And Government: Cooperation And Conflict In Red River Gorge, Eugenie C. Scott, Billie R. Dewalt, Elizabeth Adelski, Sara Alexander, Mary Beebe

KWRRI Research Reports

The research reported is based on a holistic sociocultural study of a popular regional recreation site in Eastern Kentucky, the Red River Gorge. Our research with over 3200 recreational visitors to the Gorge, 395 members of four recreation/conservation groups, 44 local landowners, and with a large number of management personnel from various governmental agencies permits us to provide an especially comprehensive overview of the problems and prospects of this popular area. Our general purpose is to provide descriptive and analytic information that will allow managers to more effectively understand and cope with their work in Red River Gorge.

In addition …

Innovations In Hay Harvesting And Storing, V. L. Lectenberg, D. A. Holt

Kentucky Alfalfa and Stored Forage Conference

Haymaking in the humid parts of the U.S. is the most risky operation that farmers engage in. Standing hay is extremely high in moisture and must be dried to at least 25% moisture less before it can be stored without spoilage. Most farmers rely on natural field drying processes to cure their hay. Natural drying is often slowed by high relative humidity. Rainfall frequently occurs before the hay has dried which further slows the drying process and can result in serious nutrient and yield losses. Haymaking is also a labor-intensive farming operation and, until recently, involved a large amount of …

Hair As An Indicator Of Excessive Aluminum Exposure, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

To determine if excessive systemic exposure to aluminum would be reflected in increased aluminum concentration in hair, rabbits were given a series of aluminum lactate injections. Hair was collected before the aluminum lactate administration from the site of injections and twice after the injections from this site as well as from an area adjoining the injection site. Aluminum was determined by flameless atomic absorption analysis of acid-digested samples. The concentration of aluminum in the hair increased after the injections in samples taken at both times from both sites. Considerable variability in hair aluminum was found before excessive exposure, as has …

Sunlight Induced Movement Of Planktonic Organisms And Their Relationships To Water Movements, John Calkins, Ed Colley, Jay Wallingford, Carol Hulsey, Kathy Lohr, Marc Bolling

KWRRI Research Reports

Previous studies have shown the planktonic organisms of various natural water bodies to be quite sensitive to the levels of solar ultraviolet radiation incident at the water surface in summer. It has been assumed that the majority of the phytoplankton are simply carried about in the mass of the water which they inhabit with little control of their position or sunlight exposure. Photosynthetic microorganisms must strike a delicate balance with regard to solar radiation; they must obtain enough visible light for adequate photosynthesis while avoiding an excessive exposure to the injurious solar UV-B radiation.

It is proposed that the circulation …

Classification And Management Of Wetlands In The Western Kentucky Coal Field, William J. Mitsch, Robert W. Bosserman, Paul L. Hill Jr., Jan R. Taylor

KWRRI Research Reports

This is the first research report of a three-year project on wetland identification and management criteria in the western Kentucky coal field. The region is approximately 12,000 square kilometers and, due to its slight relief, contains many wetlands, some contiguous with surface coal mining operations. The overall objectives of the research project are 1) to identify, classify, and map wetlands in the western Kentucky coal field; 2) to evaluate the major biotic and abiotic factors that affect those wetlands; and 3) to develop strategies for the proper management of those wetlands.

The first report of this three-year project has involved …

Effect Of Herbicides On Performance Of Aerobic Dairy Lagoons, John D. Bottom, Joseph L. Taraba

Biosystems and Agricultural Engineering Faculty Publications

The herbicides glyphosate, bromacil, and dalapon were assessed for their affect on the oxygen uptake of microorganisms from an aerated dairy lagoon at 20 °C. The maximum recommended addition of herbicide active ingerdient to a lagoon is: glyphosate (isopropyl amino salt)—0.42 mg/L; bromacil—3.70 mg/L; and dalapon (85.8 percent Na salt and 14.2 percent Mg salt)—1.95 mg/L. In addition, microorganism isolates from the aerobic dairy lagoon were incubated under aerobic and anerobic conditions at room temperature to assess the effects of the herbicides. Under aerobic incubation, it was found that neither herbicide type nor herbicide concentration affected isolate growth. Under anaerobic …

W. Hugh Peal: A Reminiscence, Lawrence S. Thompson

The Kentucky Review

No abstract provided.

Surrogate Gestation And The Protection Of Choice, Louise E. Graham

Law Faculty Scholarly Articles

Proponents of surrogate gestation contracts base their case on both the constitutional privacy rights of persons involved in the contract and the notion that contractual agreements are capable of sufficiently protecting all interests involved. This article first speculates on how courts might handle surrogate gestation contracts under existing laws and offers arguments for and against such contracts. Although some commentary on the contractual aspect of the agreement exists, little attention has been given to the privacy arguments of the parties. The major focus of this article, therefore, is upon the nature of the privacy claims asserted by the prospective parents …

Nitrification Inhibitors, Wilbur Frye

Soil Science News and Views

Nitrogen often limits the production of nonlegume crops because of inefficiency of N fertilizers. In some cases, more than half the fertilizer N may be lost from the soil. Therefore, it is important to use it as efficiently as possible. One way in which N fertilizer efficiency may be improved is by slowing the nitrification process in the soil.

Low Pressure Membrane Separation Process To Remove Heavy Metal Complexes, Dibakar Bhattacharyya, Chin-Shun Cheng

KWRRI Research Reports

The overall objective of this investigation is to establish the rejection behavior of heavy metals in the presence of complexing agents, utilizing negatively charged ultrafiltration membranes. An extensive experimental investigation is conducted with Zn²⁺, Cd²⁺, Cu²⁺, and Cu¹⁺ in the presence of cyanide, ethylenediamine tetraacetic acid, and oxalates, under insignificant concentration polarization condition. The rejection dependence of the heavy metals is found to be a function of feed metal concentration, metal types, complexing agent to metal feed molar ratio, pH and ionic strength. The dependence of rejection behavior of heavy metals and complexing …

Testing The Integrity Of Liquid Containing Hermetically Sealed Containers By The Use Of Radioactive Markers, Patrick P. Deluca, L. David Butler

Pharmaceutical Sciences Faculty Patents

A method for detecting leaky ampules filled with a liquid such as a parenteral solution. The ampules to be tested are immersed in a solution containing a short-lived radionuclide, and a pressure differential is imposed between the solution containing the radionuclide and the parenteral solution or other liquid contained in the ampules. The ampules are then removed from solution, decontaminated to remove any solution adhering to the outer surface and pores thereof, dried, and finally examined for electromagnetic radiation, preferably gamma radiation, emanating from the interior of the ampules which would indicate a leaky condition thereof.

Simulating The Water Requirements And Economic Feasibility Of Corn In The Midwest, Wendi L. Palmer, B. J. Barfield, M. E. Bitzer, C. T. Haan

KWRRI Research Reports

An evaluation of the economics of supplemental irrigation when using a surface water supply must be site specific in order to account for variations in soil moisture holding capacity, watershed area supplying the runoff, climatic conditions, and proposed irrigation management procedures.

With the use of farm specific simulation models to determine grain yields, availability of irrigation water, and economic expenditures involved in irrigation, an economic evaluation of supplemental irrigation can be performed, In the model presented in this report, the Duncan SIMAIZ model is used to predict grain yields using long-term daily weather information. SIMAIZ also determines irrigation water demand …

Go Down, Moses: Experience And The Forms Of Understanding, John Earl Bassett

The Kentucky Review

No abstract provided.

In Defense Of Surrogate Parenting: A Critical Analysis Of The Recent Kentucky Experience, John W. Phillips, Susan D. Phillips

Kentucky Law Journal

No abstract provided.

Use Of Annual Legumes As Winter Cover For No-Till Corn, Robert L. Blevins

Soil Science News and Views

Legumes in crop rotations hsve traditionally provided nitrogen for nonlegume crops since the early history of agriculture. When in association with the proper strains of Rhyzobium bacteria, legumes are capable of fixing atmospheric nitrogen, which when residues from the legumes decompose, enrich the soil content of nitrogen. Legume cover crops recycle other nutrients, thus reducing leaching losses that often occur in soils during the winter and spring seasons. In addition to these advantages, legume cover crops used with no-tillage corn provide a surface mulch which reduces soil erosion, slows evaporation of soil moisture, increases infiltration of rainfall, and increases soil …