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The Use Of A Whole Genome Scan To Find A Genetic Marker For Degenerative Suspensory Ligament Desmitis In The Peruvian Paso Horse, Diane I. Strong

University of Kentucky Master's Theses

Degenerative suspensory ligament desmitis (DSLD) is a debilitating disease of connective tissues seen in many breeds but has become prevalent in the Peruvian Pasohorse. DSLD is believed to be a genetic disorder caused by one primary founder and most likely has a recessive mode of inheritance although a dominant or co-dominant mode of inheritance has not been ruled out.

A genome scan using 259 microsatellite markers was used to test for linkage disequilibrium between one or more markers and DSLD. Two groups of Peruvian Pasohorses were selected from one population including the US and Canada. The only difference between the …

Sexual Conflict And Density Dependence In The Western Mosquitofish, Gambusia Affinis (Poeciliidae), Chad Smith

University of Kentucky Master's Theses

Sexual conflict occurs when individuals of one sex express traits that reduce the fitness of their mates. Males of many species harass females to gain copulations, which benefits males by increasing the number of offspring they sire but imposes energetic and opportunity costs on the females they harass. This thesis examined the fitness costs of sexual harassment to females, the energetic costs of mating to males, and the factors influencing the intensity of male competition for mates in the western mosquitofish Gambusia affinis. I quantified male and female behavior, four female fitness components (number of offspring per female, embryo number, …

Comparative Analysis Of Five Complete Ambystomatid Salamander Mitochondrial Genomes, Amy K. Samuels

University of Kentucky Master's Theses

In this study, mitochondrial transcript information from a recent EST project wasextended to obtain complete mitochondrial genome sequence for 5 tiger salamandercomplex species (Ambystoma mexicanum, A. t. tigrinum, A. andersoni, A. californiense,and A. dumerilii). For the first time, aspects of mitochondrial transcription in arepresentative amphibian are described, and then complete mitochondrial sequencedata are used to examine salamander phylogeny at both deep and shallow levels ofevolutionary divergence. The available mitochondrial ESTs for A. mexicanum (N=2481)and A. t. tigrinum (N=1205) provided 92% and 87% coverage of the mitochondrialgenome, respectively. Complete mitochondrial sequences for all species were rapidlyobtained by using long distance PCR …

Predicting The Location And Duration Of Transient Induced Low Or Negative Pressures Within A Large Water Distribution System, Richard C. Svindland

University of Kentucky Master's Theses

Surge modeling is a tool used by engineers and utility owners in determining the surge pressures or transients that may result from routine pump and valve operations. Recent surge modeling work has focused on low and/or negative pressures within water distribution systems and how those occurrences could lead to intrusions. Effective surge modeling is needed in order to determine if the intrusion potential exists and what mitigation is needed to prevent intrusions. This work focuses on the generally unexplored area of using surge models to predict the location and duration of transient induced low and/or negative pressures within large complex …

Phylogenetic Analysis Of Kentucky Strains Of Xylella Fastidiosa, J. Nicole Mundell

University of Kentucky Master's Theses

Phytopathogenic bacterium, Xylella fastidiosa, causes a number of economically important diseases, including Pierces disease (PD) of grape and bacterial leaf scorch (BLS) of a number of landscape trees. In Kentucky (KY), BLS affects a number of shade trees including many oak and maple species. In 2001, PD was diagnosed in grapevines in western KY. Xylella fastidiosa is also detected in many asymptomatic landscape plants and grasses. It was the goal of this research to identify hosts of X. fastidiosa around KY and use phylogenetic analysis to compare sequences of the 16S rDNA and gyrase B (gyrB) genes between samples. This …

Using Sequential Imagery To Evaluate Aspects Of Seed Vigor And Germination, Manjul Dutt

University of Kentucky Master's Theses

A sequential imaging system using a flat bed scanner interfaced to a personal computer was developed to study seed germination and vigor. The utility of the system was demonstrated in studies relating aspects of seed vigor in impatiens seed lots and for dormancy release in two woody perennials. Impatiens seed lots were kept in storage for 6 months to obtain a range in vigor levels. The ability to see differences at the individual seed level was possible because of the development of the sequential imaging system. It was observed that seeds deteriorated both under optimum 4OC as well as 25OC …

An Examination Of Dietary Amendments To Affect Phosphorus Utilization In Growing Pigs, Jorge Hernan Agudelo-Trujillo

University of Kentucky Doctoral Dissertations

For economical and ecological reasons, efficiency and profitability ofswine production relies heavily on the way pigs utilize key nutrients such as P,which is considered a potential pollutant of water ecosystems. Although cerealgrains and oilseed meals contain enough P to fulfill the biological needs of pigs,most of this P is tightly bound as phytate. As pigs do not have enough phytase(PHY) to cleave P from phytate, it is excreted in the feces. To prevent adeficiency, diets have traditionally been supplemented with highly availableinorganic sources of P. Today, an environmentally-friendly alternative is tosupplement diets with PHY.Growth promoting antibiotics are also used to …

Some Effects Of Cadmium On Select Crayfish In The Family Cambaridae, Andrew Joseph Wigginton

University of Kentucky Doctoral Dissertations

A series of acute (96h) toxicity tests were conducted on six species of crayfish inthe family Cambaridae. Toxicity values fell into a sensitive group comprising Orconectesplacidus and Procambarus acutus (LC 50= 0.368mg Cd/L - 0.487mg Cd/L; LC10= 0.048mg Cd/L - 0.092 mg Cd/L) and tolerant group comprising O. juvenilis, O. virilis, P.alleni, and P. clarkii (LC 50= 2.44 mg Cd/L - 3.30 mg Cd/L; LC10= 0.386 mg Cd/L -0.947 mg Cd/L). For juvenile crayfish, the LC50 and LC10 values were as follows: O.juvenilis, 0.060 and 0.014 mg Cd/L; O. placidus, 0.037 and 0.002 mg Cd/L; P. clarkii,0.624 and 0.283 mg …

Studies Of Unusual Packing And Of Polymorphism In Two Crystal Systems, Xiang Hao

University of Kentucky Doctoral Dissertations

Crystal structures of anhydrous pinacol, the hexagonal pinacol, pinacol monohydrate, and pinacol hexahydrate were studied. In all the structures crystal packing is unusual and complicated. The origin of the complexity may be the difficulty in filling space densely and while also satisfying the H-bonding requirements when the molecule has few internal degrees of freedom.

Five 15-crown-5 complexes of M(NO₃)₂ (M = Cu, Zn, Mg, Co, Mn) were synthesized and characterized using X-ray diffraction and differential scanning calorimetry. The system is rich in polymorphs. Nine definite solid-state phases were identified. More phases probably exist in the solid state …

Redox Proteomics Identification Of Oxidatively Modified Proteins And Their Pharmacological Modulation: Insight Into Oxidative Stress In Brain Aging, Age-Related Cognitive Impairment, Hung Fai Poon

University of Kentucky Doctoral Dissertations

The studies presented in this work were completed with the goal ofgaining greater insight into the roles of protein oxidation in brain aging and age-relatedcognitive impairment. Aging is associated with the impairment of physiological systemssuch as the central nervous system (CNS), homeostatic system, immune system, etc.Functional impairments of the CNS is associated with increased susceptibility to developmany neurodegenerative diseases such as Alzheimer's diseases (AD), Parkinson's disease(PD), and amyotrophic lateral sclerosis (ALS). One of the most noticeable functionalimpairments of the CNS is manifested by cognitive decline. In the past three decades, thefree radical theory of aging has gained relatively strong support …

Pragmatism And The Politics Of Rewilding Nature: The Case Of Grizzly Bear Reintroduction In Idaho, John G. Hintz

University of Kentucky Doctoral Dissertations

In 1975, the US Fish and Wildlife Service listed the grizzly bear as a threatened species under the Endangered Species Act. Following the listing, a recovery plan was drafted in which the Bitterroot Ecosystem of central Idaho and extreme western Montana was one of six proposed grizzly bear recovery areas. It was the only one of the six, however, which did not contain a resident population of grizzlies. The Fish and Wildlife Service eventually accepted a proposal submitted by a coalition of environmental and timber industry groups. The coalition proposed to reestablish a population of grizzlies in the Bitterroot by …

Modulation Of The Adrenal Medullary Response To Stress By Estradiol In The Female Rat, Julye Marie Adams

University of Kentucky Doctoral Dissertations

The present study has established that physiological concentrations of estradiol can modulate stress-induced increases in plasma epinephrine (EPI). In anesthetized female rats, insulin-induced hypoglycemia (0.25 U/kg) increased plasma EPI concentration to a significantly greater extent in 14-day ovariectomized (OVEX) rats compared to sham-operated controls. In 17-estradiol (E2)-replaced OVEX rats, the hypoglycemia-induced rise in plasma EPI was significantly reduced compared to OVEX rats. This suppression was due to both decreased adrenal medullary output and increased clearance of EPI. Adrenal venous EPI concentration was significantly reduced in OVEX+E2 rats, suggesting that EPI secretion from the adrenalmedulla was decreased by E2 replacement. The …

Mechanism Of Cancer Selective Apoptosis By Par-4, Sushma Gurumurthy

University of Kentucky Doctoral Dissertations

Despite distinct dissimilarities, diverse cancers express several common pro-tumorigenic traits. We present here evidence that the pro-apoptotic protein Par-4 utilizes one such common tumorigenic trait to become selectively activated and induce apoptosis in cancer cells. Elevated PKA activity noted in cancer cells activated the apoptotic function of ectopic Par-4 or its SAC domain, which induces apoptosis selectively in cancer cells and not in normal or immortalized cells. PKA preferentially phosphorylated Par-4 at the T155 residue within the SAC domain in cancer cells. Moreover, pharmacological-, peptide- or siRNA-mediated inhibition of PKA activity in cancer cells resulted in abrogation of both T155 …

Streambank Erosion Associated With Grazing Practices In The Humid Region, Carmen T. Agouridis, Dwayne R. Edwards, Stephen R. Workman, José R. Bicudo, Benjamin K. Koostra, Eric S. Vanzant, Joseph L. Taraba

Biosystems and Agricultural Engineering Faculty Publications

The effects of cattle grazing on stream stability have been well documented for the western portion of the U.S., but are lacking for the east. Stream and riparian damage resulting from grazing can include alterations in watershed hydrology, changes to stream morphology, soil compaction and erosion, destruction of vegetation, and water quality impairments. However, few studies have examined the successes of best management practices (BMPs) for mitigating these effects. The objective of this project was to assess the ability of two common BMPs to reduce streambank erosion along a central Kentucky stream. The project site consisted of two replications of …

Securing The Commonwealth: Changing Emergency Preparedness In Kentucky: A Study Of The Efforts Of The Department Of Homeland Security In Changing Emergency Preparedness At The County Level, Christopher Cross

MPA/MPP/MPFM Capstone Projects

Statement of the Problem

During the first 24 to 72 hours following a terrorist attack, local officials and first responders will be responsible for dealing with the initial aftermath. While most officials—federal, state, and local—have all acknowledged that domestic preparedness must occur at the bottom of the governmental structure, it is unclear if any changes to emergency response planning have actually occurred at the local governmental level.

Research Questions

The intention of this capstone is to answer the following questions:

1. Has change in emergency response planning occurred at the county level?

2. Are there regional differences present in terms of organizational …

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Effects Of Glial Cell Line-Derived Neurotrophic Factor (Gdnf) On Stem/Progenitor Cell Proliferation And Differentiation, Yan Chen

University of Kentucky Doctoral Dissertations

Stem/progenitor cells are present in the adult brain; they undergo constantproliferation and differentiate into mature neurons in certain brain areas, a phenomenoncalled neurogenesis. This study investigated the effects of GDNF, a potent trophic factorof dopaminergic neurons, on neurogenesis in the brain. Nestin and 5-Bromo-2'-deoxyuridine (BrdU) were used as stem/progenitor cells markers.First, we observed extensive bilateral increases of stem/progenitor cells in thedentate gyrus and substantia nigra after continuous infusion of GDNF into the normal ratbrain. However, none of the BrdU+ cells showed neuronal features in the substantia nigraas characterized by immunocytochemical procedures. Next, we identified themorphology of BrdU+ cells after infusing …

A Comparison Between Masticatory Muscle And Temporomandibular Joint Pain Patients With Regard To The Prevalence And Impact Of Post-Traumatic Stress Disorder Symptoms, Elizangela Bertoli

University of Kentucky Master's Theses

The purpose of this study was to evaluate masticatory muscle (MM) and temporomandibular joint (TMJ) pain patients regarding the prevalence of Posttraumatic Stress Disorder (PTSD) symptoms, and evaluate the level of psychological dysfunction and its relationship to PTSD symptoms in these patients. This study included 445 adult patients (male = 42, female = 403). Psychological questionnaires included the Symptom Check List-90-Revised (SCL-90-R), the Multidimensional Pain Inventory, the Pittsburgh Sleep Quality Index and the PTSD Check List Civilian. The total sample of patients was divided into two major groups: The MM group (n=242) and TMJ group (n=203). Each group was divided …

Design Of A Gait Acquisition And Analysis System For Assessing The Recovery Of Mice Post-Spinal Cord Injury, Casey Harr

University of Kentucky Master's Theses

Current methods of determining spinal cord recovery in mice, post-directed injury, are qualitative measures. This is due to the small size and quickness of mice. This thesis presents a design for a gait acquisition and analysis system able to capture the footfalls of a mouse, extract position and timing data, and report quantitative gait metrics to the operator. These metrics can then be used to evaluate the recovery of the mouse. This work presents the design evolution of the system, from initial sensor design concepts through prototyping and testing to the final implementation. The system utilizes a machine vision camera, …

Development Of Protein-Imprinted Polysiloxane Biomaterials: Protein Selectivity And Cellular Responses, Kyoungmi Lee

University of Kentucky Master's Theses

Surface modification is an extensively researched approach in order to overcomethe limitations, and improve the performance of orthopedic and dental implants. It is atthe surface of the implant materials that the initial interactions of tissues or body fluidstake place. Therefore, surface properties of biomaterials are the important factors that cancontrol these biological responses. Molecular imprinting is a surface modificationtechnique that creates specific recognition sites on the surface of biomaterials. Todevelop the recognition sites, a functional monomer is assembled with templatebiomolecule and then crosslinked. After removal of the template, the surface can rebindthe molecules. Therefore, desired reactions can be initiated at …

Bone Engineering Of The Ulna Of Rabbit, Amanda Peter Hart

University of Kentucky Master's Theses

Repair of bone defects is a major challenge in orthopaedic surgery. Current bone graft treatments, including autografts, allografts and xenografts, have many limitations making it necessary to develop a biomaterial to be a bone graft substitute. One such biomaterial is bioactive resorbable silica-calcium phosphate nanocomposite (SCPC). SCPC was processed using a 3D rapid prototyping technique and sintered at different temperatures to create porous scaffolds. SEM analyses and mercury intrusion porosimetry showed SCPC to be highly porous with micro- and nanopores. BET analysis indicated that SCPC had high surface area. Mechanical testing demonstrated that SCPC had a compressive strength similar to …

Kentucky Feeder Cattle Price Analysis: Models For Price Predictions And Grazing Management, Roger Wayne Eldridge

University of Kentucky Master's Theses

Kentucky plays an important role in the complex U.S. beef cattle industry. Thisstudy focused on the feeder cattle production sector of Kentucky's beef cattle industry.Primarily a cow-calf state with a substantial backgrounding sector, Kentucky is a largesupplier of feeder cattle to the cattle finishing sector. Price relationships within themarket for Kentucky feeder cattle were examined using historical price data fromKentucky livestock auction markets. This research revealed many interesting pricerelationships that Kentucky producers may use in order to increase the profitability of thecow-calf and/or backgrounding operations. A segment of this research includes aGrazing Management Decision Tool which was constructed to enable …

Group I Intron-Derived Ribozyme Reactions, Ashley Kirtley Johnson

University of Kentucky Doctoral Dissertations

Group I introns are catalytic RNAs capable of self-splicing out of RNA transcripts. Ribozymes derived from these group I introns are used to explore the molecular recognition properties involved in intron catalysis. New ribozyme reactions are designed based on the inherent ability of these ribozymes to perform site-specific nucleophilic attacks. This study explores the molecular recognition properties of group I intron-derived ribozyme reactions and describe a new ribozyme reaction involving molecular recognition properties previously not seen.We report the development, analysis, and use of a new combinatorial approach to analyze the substrate sequence dependence of suicide inhibition, cyclization, and reverse cyclization …

Lives Under Construction: A Study Of College Sophomores, Elizabeth L. Morley

University of Kentucky Doctoral Dissertations

As individuals, college students make choices that both reflect their past lives and constitute their futures. In this research I examine the ways five college sophomores built their lives in the complex world of a research university campus. Using case study analysis I look at how the students negotiated the considerable academic and social demands of their daily lives. College impact models and literature about theories of practice and decision making inform the analysis. Human agency and fields of practice help to explain the behaviors of these students. The research reveals that students take a myriad of paths to negotiate …

Exogenous Enzymes And Organic Acids In The Nutrition Of Broiler Chicks: Effects On Growth Performance And In Vitro And In Vivo Digestion, Tuoying Ao

University of Kentucky Doctoral Dissertations

Studies were conducted to investigate the interactive effects of exogenous enzymes andorganic acids on in vitro and in vivo nutrient digestion and growth performance of broiler chicks.In Study 1, five exogenous enzyme products including ??-glucanase, xylanase, amylase, ??-galactosidase and protease, were assayed in triplicate at their optimum pH levels and at pH levelsof 3.0, 6.0, 6.5, 7.0 and 7.5, which were used to simulate pH levels found in the gizzard, the diet,the crop, and the proximal and distal parts of small intestine, respectively. The pH gradient wasobtained by dissolving the enzymes in different buffers. Results suggested that the pH levelscommonly …

In Vitro And In Vivo Characterization Of A Trans Excision-Splicing Ribozyme, Dana Ann Baum

University of Kentucky Doctoral Dissertations

Group I introns are catalytic RNAs with the ability to splice out of RNA transcripts, often without the aid of proteins. These self-splicing introns have been reengineered to create ribozymes with the ability to catalyze reactions. One such ribozyme, derived from a Pneumocystis carinii group I intron, has been engineered to sequence specifically remove a targeted segment from within an RNA substrate, which is called the trans excision-splicing reaction.The two catalytic steps of the trans excision-splicing reaction occur at positions on the substrate known as the 5' and 3' splice sites. Strict sequence requirements at these sites could potentially limit …

Genetic Diversity Of Bean Pod Mottle Virus (Bpmv) And Development Of Bpmv As A Vector For Gene Expression In Soybean, Chunquan Zhang

University of Kentucky Doctoral Dissertations

Bean pod mottle virus (BPMV), a member of the genus Comovirus in the family Comoviridae, is widespread in the major soybean-growing areas in the United States. The complete nucleotide sequences of the genomic RNAs of the naturally occurring partial diploid strain IL-Cb1 were determined. Intermolecular RNA1 recombinants were isolated from strain IL-Cb1 and characterized at the molecular level. Structurally similar recombinant RNA1 was also generated after four passages in soybean derived from plants previously inoculated with a mixture of infectious RNA1 transcripts from two distinct strains. BPMV was developed as a plant viral vector that is appropriate for gene expression …

Neural Network Applications In Agricultural Economics, Jianhua Chen

University of Kentucky Doctoral Dissertations

Neural networks have become very important tools in many areas including economic researches. The objectives of this thesis are to examine the fundamental components, concepts and theory of neural network methods from econometric and statistic perspective, with particular focus on econometrically and statistically relevant models. In order to evaluate the relative effectiveness of econometric and neural network methods, two empirical studies are conducted by applying neural network methods in a methodological comparison fashion with traditional econometric models.Both neural networks and econometrics have similar models, common problems of modeling and interference. Neural networks and econometrics/statistics, particularly their discriminant methods, are two …

Hierarchical Hybrid-Model Based Design, Verification, Simulation, And Synthesis Of Mission Control For Autonomous Underwater Vehicles, Siddhartha Bhattacharyya

University of Kentucky Doctoral Dissertations

The objective of modeling, verification, and synthesis of hierarchical hybrid mission control for underwater vehicle is to (i) propose a hierarchical architecture for mission control for an autonomous system, (ii) develop extended hybrid state machine models for the mission control, (iii) use these models to verify for logical correctness, (iv) check the feasibility of a simulation software to model the mission executed by an autonomous underwater vehicle (AUV) (v) perform synthesis of high-level mission coordinators for coordinating lower-level mission controllers in accordance with the given mission, and (vi) suggest further design changes for improvement. The dissertation describes a hierarchical architecture …

Role Of Matrix Metalloproteinase-2 In Therosclerosis And Abdominal Aortic Aneurysms In Apolipoprotein E Deficient Mice, Jing Huang

University of Kentucky Doctoral Dissertations

Matrix metalloproteinase-2 (MMP-2, gelatinase A, type IV collagenase) is a member of a family of zinc-dependent metalloendopeptidases that functions in the degradation of elastin, collagens, and other components of extracellular matrix (ECM). Both secretion and activation of MMP-2 are elevated in human atherosclerotic lesions and abdominal aortic aneurysms (AAA). In this dissertation project, we sought to test the hypothesis that MMP-2 plays a critical role in both atherosclerosis and AAA. We also sought to determine the detailed mechanism. We first examined the atherosclerosis and AngII-induced AAAs development in MMP-2-/- x apolipoprotein (apoE)-/- mice in vivo. It was surprising that MMP-2 …

Interactions Between Selenium And Polychlorinated Biphenyls (Pcbs), Divinia Nolasco Stemm

University of Kentucky Doctoral Dissertations

This study investigated the interaction between polychlorinated biphenyls (PCBs) and selenium to explain the mechanism involved that could affect selenium metabolism and its anti-cancer property. PCBs congeners and mixtures were previously found to reduce hepatic Se and Se-dependent glutathione peroxidase activity. I hypothesized that certain PCB congeners affect selenium metabolism in the rat liver resulting in diminished antioxidant capacity of selenoproteins, which could alter the ability of Se to protect against PCBs induced tumor promotion. In the first study, the influence of 3,3,4,4-tetrachlorobiphenyl (PCB 77) on hepatic Se and glutathione peroxidase (GPx1) activity as well as cytochrome P450 1A1 induction …