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Shelling Out For Genomics, Timothy S. Mcclintock, Charles D. Derby

Physiology Faculty Publications

A report on the symposium 'Genomic and Proteomic Approaches to Crustacean Biology' held as part of the Society for Integrative and Comparative Biology 2006 Annual Meeting, Orlando, USA, 4-8 January 2006.

Iodine Uptake Restoration In Thyroid Cancer, Kenneth B. Ain, Gopalakrishnan Venkataraman

Internal Medicine Faculty Patents

A method of expressing a tumor specific therapeutic response element in a cancerous cell is disclosed in which the response element was previously blocked from expression. The method comprises the step of administering an unblocking agent to the cancerous cell harboring a gene encoding the response element, thereby resulting in the expression of the response element.

Prenatal Cocaine Exposure Alters Alpha2 Receptor Expression In Adolescent Rats, Rosemarie M. Booze, David R. Wallace, Janelle M. Silvers, Barbara J. Strupp, Diane M. Snow, Charles F. Mactutus

Neuroscience Faculty Publications

BACKGROUND: Prenatal cocaine exposure produces attentional deficits which to persist through early childhood. Given the role of norepinephrine (NE) in attentional processes, we examined the forebrain NE systems from prenatal cocaine exposed rats. Cocaine was administered during pregnancy via the clinically relevant intravenous route of administration. Specifically, we measured alpha2-adrenergic receptor (alpha2-AR) density in adolescent (35-days-old) rats, using [3H]RX821002 (5 nM).

RESULTS: Sex-specific alterations of alpha2-AR were found in the hippocampus and amygdala of the cocaine-exposed animals, as well as an upregulation of alpha2-AR in parietal cortex.

CONCLUSION: These data suggest that prenatal cocaine exposure results in a persistent alteration …

Disclosure Interviews David Buss. Evolutionary Psychology And Intimacy: The Science Of Violence, Competition, And Sex

disClosure: A Journal of Social Theory

No abstract provided.

Artistic Expression, Intimacy, And The Primal Holon: Sam Shepard's Suicide In B-Flat And The Tooth Of Crime, William S. Haney Ii

disClosure: A Journal of Social Theory

No abstract provided.

Spontaneous Intracranial Arterial Dissection In The Young: Diagnosis By Ct Angiography, William C. Robertson, Curtis A. Given

Neurology Faculty Publications

BACKGROUND: Spontaneous carotid artery dissections have been rarely reported in children. Diagnosis has traditionally been confirmed by catheter arteriography. More recently diagnosis has been made by magnetic resonance imaging and magnetic resonance angiography; however the sensitivity of these techniques has yet to be determined. The authors are unaware of reports of carotid dissection confirmed by dynamic computed tomography (computerized tomographic arteriography) in the young.

CASE PRESENTATION: We recently evaluated a fourteen year-old male following the development of transient neurologic symptoms. There was no antecedent illness or trauma. Dynamic computed tomography revealed an intracranial dissection involving the supraclinoid segment of the …

Biodiesel Faq, Timothy S. Stombaugh, Czarena L. Crofcheck, Michael D. Montross

Agricultural Engineering Extension Publications

Biodiesel and other alternative fuels continue to gain popularity as petroleum fuel prices rise and we become more concerned about our environment. Introduction of these fuels raises many questions about actually using them in current equipment. The purpose of this factsheet is to address some of the common questions asked by those considering the use of biodiesel in existing diesel equipment.

Racial Disparity And Socioeconomic Status In Association With Survival In Older Men With Local/Regional Stage Prostate Cancer: Findings From A Large Community-Based Cohort, Xianglin L. Du, Shenying Fang, Ann L. Coker, Corinne Aragaki, Janice N. Cormier, Yan Xing, Beverly J. Gor, Wenyaw Chan

CRVAW Faculty Journal Articles

BACKGROUND

Few studies have examined the outcomes for Hispanic men with prostate carcinoma and incorporated socioeconomic factors in association with race/ethnicity in affecting survival, adjusting for factors on cancer stage, grade, comorbidity, and treatment.

METHODS

We studied a population-based cohort of 61,228 men diagnosed with local or regional stage prostate carcinoma at age 65 years or older between 1992 and 1999 in the 11 SEER (Surveillance, Epidemiology, and End Results) areas, identified from the SEER-Medicare linked data with up to 11 years of followup.

RESULTS

Low socioeconomic status was significantly associated with decreasing survival in all men with prostate carcinoma. …

Holistic Health: Does It Really Include Mental Health?, Kimberly K. Mcclanahan, Marlene B. Huff, Hatim A. Omar

Pediatrics Faculty Publications

Holistic health, incorporating mind and body as equally important and unified components of health, is a concept utilized in some health care arenas in the United States (U.S.) over the past 30 years. However, in the U.S., mental health is not seen as conceptually integral to physical health and, thus, holistic health cannot be realized until the historical concept of mind-body dualism, continuing stigma regarding mental illness, lack of mental health parity in insurance, and inaccurate public perceptions regarding mental illness are adequately addressed and resolved. Until then, mental and physical health will continue to be viewed as disparate entities …

Clinical Holistic Medicine: Holistic Sexology And Acupressure Through The Vagina (Hippocratic Pelvic Massage), Søren Ventegodt, Birgitte Clausen, Hatim A. Omar, Joav Merrick

Pediatrics Faculty Publications

Many gynecological and sexological problems (like urine incontinence, chronic pelvic pains, vulvodynia, and lack of lust, excitement, and orgasm) are resistant to standard medical treatment. In our work at the Research Clinic for Holistic Medicine in Copenhagen, we have found that vaginal acupressure, or Hippocratic pelvic massage, can help some of these problems. Technically, it is a very simple procedure as it corresponds to the explorative phase of the standard pelvic examination, supplemented with the patient's report on the feelings it provokes and the processing and integration of these feelings. Sometimes it can be very difficult to control the emotions …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Limestone-Based Material for Arsenic Removal from Drinking Water, Chelsea Campbell and others, Dept of Chemistry, WKU
• Opportunistic Water Education, Amanda Abnee Gumbert, Extension Associate for Environmental and Natural Resources Issues, UK
• Ten-Year Solute Concentration Patterns in Two Streams of Contrasting Land-Use in Western Kentucky and Tennessee, Susan P. Hendricks, Hancock Biological Station, Murray State University
• Pathogen TMDL Development using Load Duration Curves for Two Stream Segments in Rockcastle County, Kentucky, Joseph M. Ferguson, Kentucky DOW
• Beargrass Creek Water Quality Tool and TMDLs, Ward Wilson and others, Tetra Tech
• Pathogen and Sediment Transport in Muddy Creek …

Session 1d: Monitoring, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• An Examination of Statewide Watershed Watch Project Monitoring Data for 2005 Using GIS, Ken Cooke, Kentucky Division of Water, Frankfort
• Taking Watershed Watch to the Next Level: Grabbing the Attention of Local Elected Officials, Ken Cooke, Kentucky Division of Water, H. David Gabbard, LFUCG, Lexington
• Water Quality Data from Citizen Monitoring in the Licking River Region Kentucky 1998-2005: Trends and Issues, M.F. Hult, Daniel Carter Beard Environmental Center, and B.C. Reeder, Center for Environmental Education, Morehead State University
• Water Quality in the Upper Licking River Basin 2003-2005, Brian Reeder and others, Dept of Biological and Environmental …

Session 1c: Superfund, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• The University of Kentucky Superfund Basic Research Program: Overview and Examples of Research Projects, Bernhard Hennig, Leonidas Bachas, and Lindell Ormsbee, UK
• Reductive Dechlorination of Toxic Organics by Bimetallic Nanoparticles in Polyacrylic Acid (PAA) Functionalized MF Membranes, Jian Xu and Dibakar Bhattacharyya, Dept Chemical and Materials Engineering, UK
• Chlorinated Organic Compounds Destruction by Modified Fenton Reaction Involving Immobilized Iron-Chelate, YongChao Li, Leonidas Bachas, and Dibakar Bhattacharyya, Dept Chemical and Materials Engineering, UK
• An Outreach Program Translates Basic Research for Superfund Communities to Improve Health through Nutrition, Lisa Gaetke and Sandra Bastin, Dept Nutrition and Food Science, …

Session 1a: Water Quality, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Use of Sunfish and Stoneroller Minnows as Sentinel Monitors of PCB Contamination in Freshwater Streams in Kentucky, D.J. Price and W.J. Birge, Dept of Biology, UK
• Inferring Causes of Biological Impairment in Appalachian Streams: Watershed-Based Problem Formulation and Integration of Multiple Lines of Evidence, Jon Ludwig and others, Tetra Tech
• Relative Importance of Water and Dietary Cadmium: Toxicity to Ceriodaphnia Dubia, Agus Sofyan and W.J. Birge, Dept of Biology, UK
• The Graywater Story at Curtis Pike, Jack Kieffer and others, Appalachia - Science in the Public Interest, Mt. Vernon

Proceedings Of 2006 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This symposium was funded in part by the U.S. Environmental Protection Agency, with Clean Water Act, Section 319(h) grant money through the Kentucky Division of Water and the Kentucky Waterways Alliance, #C9994861-00.

Planning for this conference was conducted as part of the state water resources research annual program with the support and collaboration of the Department of Interior, U.S. Geological Survey and the University of Kentucky Research Foundation, under Grant Agreement No. 01HQGR0133.

The views and conclusions contained in this document are those of the abstract authors and should not be interpreted as necessarily representing the official policies, either expressed …

[Review Of] Stable Isotopes And Biosphere–Atmosphere Interactions: Processes And Biological Controls, Mark S. Coyne

Plant and Soil Sciences Faculty Publications

No abstract provided.

Length-Dependent Degradation Of Single-Stranded 3' Ends By The Werner Syndrome Protein (Wrn): Implications For Spatial Orientation And Coordinated 3' To 5' Movement Of Its Atpase/Helicase And Exonuclease Domains, Amrita Machwe, Liren Xiao, David K. Orren

Toxicology and Cancer Biology Faculty Publications

BACKGROUND: The cancer-prone and accelerated aging disease Werner syndrome is caused by loss of function of the WRN gene product that possesses ATPase, 3' to 5' helicase and 3' to 5' exonuclease activities. Although WRN has been most prominently suggested to function in telomere maintenance, resolution of replication blockage and/or recombinational repair, its exact role in DNA metabolism remains unclear. WRN is the only human RecQ family member to possess both helicase and exonuclease activity, but the mechanistic relationship between these activities is unknown. In this study, model single-stranded and 3' overhang DNA substrates of varying length and structure were …

Cbfbeta Is A Facultative Runx Partner In The Sea Urchin Embryo, Anthony J. Robertson, Carrie Dickey-Sims, Andrew Ransick, Dawn E. Rupp, John J. Mccarthy, James A. Coffman

Physiology Faculty Publications

BACKGROUND: Runx proteins are developmentally important metazoan transcription factors that form a heterodimeric complex with the non-homologous protein Core Binding Factor beta (CBFbeta). CBFbeta allosterically enhances Runx DNA binding but does not bind DNA itself. We report the initial characterization of SpCBFbeta, the heterodimeric partner of SpRunt-1 from the sea urchin Stronylocentrotus purpuratus.

RESULTS: SpCBFbeta is remarkably similar to its mammalian homologues, and like them it enhances the DNA binding of the Runt domain. SpCBFbeta is entirely of zygotic provenance and its expression is similar that of SpRunt-1, accumulating globally at late blastula stage then later localizing to endoderm and …

Loss Of Sparc-Mediated Vegfr-1 Suppression After Injury Reveals A Novel Antiangiogenic Activity Of Vegf-A, Miho Nozaki, Eiji Sakurai, Brian J. Raisler, Judit Z. Baffi, Jassir Witta, Yuichiro Ogura, Rolf A. Brekken, E Helene Sage, Balamurali K. Ambati, Jayakrishna Ambati

Ophthalmology and Visual Science Faculty Publications

VEGF-A promotes angiogenesis in many tissues. Here we report that choroidal neovascularization (CNV) incited by injury was increased by excess VEGF-A before injury but was suppressed by VEGF-A after injury. This unorthodox antiangiogenic effect was mediated via VEGFR-1 activation and VEGFR-2 deactivation, the latter via Src homology domain 2-containing (SH2-containing) tyrosine phosphatase-1 (SHP-1). The VEGFR-1-specific ligand placental growth factor-1 (PlGF-1), but not VEGF-E, which selectively binds VEGFR-2, mimicked these responses. Excess VEGF-A increased CNV before injury because VEGFR-1 activation was silenced by secreted protein, acidic and rich in cysteine (SPARC). The transient decline of SPARC after injury revealed a temporal …

Methods To Identify Plant Metabolites, Deane L. Falcone, John M. Littleton

Plant and Soil Sciences Faculty Patents

This invention provides materials and methods to manipulate the plant genome at the level of single plant cells in culture resulting in the ability to assign metabolic functionality to plant genes involved in the production of biologically active molecules and to create a means of product discovery based on the biosynthetic capacity of plants. The materials to create an activation mutagenesis include incorporation of enhancer sequences from a plant viral promoter at random places in the plant genome via Agrobacterium mediated DNA transfer (T-DNA). The usefulness is that genes in the immediate vicinity of the incorporation were activated which allows …

Competition Between The Dna Unwinding And Strand Pairing Activities Of The Werner And Bloom Syndrome Proteins, Amrita Machwe, Enerlyn M. Lozada, Liren Xiao, David K. Orren

Toxicology and Cancer Biology Faculty Publications

BACKGROUND: The premature aging and cancer-prone Werner and Bloom syndromes are caused by defects in the RecQ helicase enzymes WRN and BLM, respectively. Recently, both WRN and BLM (as well as several other RecQ members) have been shown to possess a strand annealing activity in addition to the requisite DNA unwinding activity. Since an annealing function would appear to directly oppose the action of a helicase, we have examined in this study the dynamic equilibrium between unwinding and annealing mediated by either WRN or BLM.

RESULTS: Our investigation into the competition between annealing and unwinding demonstrates that, under standard reaction …

Does Food Stamp Receipt Mediate The Relationship Between Food Insufficiency And Mental Health?, Colleen Heflin, James P. Ziliak

University of Kentucky Center for Poverty Research Discussion Paper Series

Although the Food Stamp Program is the largest entitlement program remaining in the social safety net, comparatively little is known about the potential benefits that the program may confer on recipients. In this paper we examine an important dimension of well being, mental health, and the extent to which participation in the Food Stamp Program may attenuate the effect of food insufficiency on levels of emotional distress. Using longitudinal data from a nationally representative sample of families in the Panel Study of Income Dynamics (PSID) we model emotional distress as a function of food insufficiency and other known risk factors …

A Case-Control Study Of Farming And Prostate Cancer In African-American And Caucasian Men, Tamra E. Meyer, Ann L. Coker, Maureen Sanderson, Elaine Symanski

CRVAW Faculty Journal Articles

Objective: To determine the risk of prostate cancer associated with farming by duration, recency and specific activities among African-Americans and Caucasians.

Methods: This population-based case–control study had information on farming-related activities for 405 incident prostate cancer cases and 392 controls matched for age, race and region in South Carolina, USA, from 1999 to 2001. Cases with histologically confirmed, primary invasive prostate cancer who were aged between 65 and 79 years were ascertained through the South Carolina Central Cancer Registry. Appropriately matched controls were identified from the Health Care Financing Administration Medicare Beneficiary File. Data were collected using computer-assisted telephone interviewing, …

Nitrapyrin (N-Serve) With Anhydrous Ammonia At/Near Corn Planting, John H. Grove

Agronomy Notes

Higher prices for energy are driving corn fertilizer nitrogen (N) prices higher as well. This increases interest in alternative management practices and products that optimize corn’s N nutrition, but maintain grower profit. Alternatives permitting fertilizer N rate reductions include: a) changes in N application timing and placement; b) using alternative sources of N (ex. poultry litter) to meet part of corn’s N need; and c) using fertilizer N additives that improve N use efficiency by inhibiting one or more N loss processes in the soil N cycle (biological N transformations).

Vertical Loads Due To Wheat On Obstructions Located On The Floor Of A Model Bin, Marek Molenda, Michael D. Montross, Sidney A. Thompson, Jozef Horabik

Biosystems and Agricultural Engineering Faculty Publications

Tests were conducted in a model grain bin to evaluate the vertical loads acting on differently shaped obstructions embedded in wheat during filling, detention, and discharge. The bin had corrugated galvanized steel walls with a 1.83 m diameter and a flat bottom. All tests were conducted in a bin that was centrically loaded and unloaded. Three differently shaped obstructions (disc, cone, and cylinder) were tested; each had a circular base equivalent to 6% of the bin floor area. The obstructions were supported in the bin using a three-legged support structure. Each leg of the support structure rested on a load …

Assessment Of Water Quality Trends In The Upper Cumberland River Basin: Focus On Pathogen Impairment, Kentucky Water Resources Institute

KWRRI Research Reports

This project consisted of analyzing existing water quality data for streams in the Upper Cumberland River Basin, with a particular focus on pathogen trends. According to the most recent 303(d) list of Kentucky waters, the majority of streams assessed in the Upper Cumberland Basin are impaired for primary recreational contact as a result of pathogens. The state pathogen standard for primary contact recreation is expressed in a dual form which specifies that the 30-day geometric mean of fecal coliform counts not exceed 200 colonies per 100 mL (on a minimum of five samples) and not more than 20 percent of …

Kentucky Water Resources Research Institute Annual Technical Report Fy 2005, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Annual Technical Reports (USGS’s 104b Grant Program)

The 2005 Annual Technical Report for Kentucky consolidates reporting requirements of the Section 104(b) base grant award in a single technical report that includes: 1) a synopsis of each research project supported during the period, 2) related reports and publications, 3) a description of information transfer activities, 4) a summary of student support during the reporting period, and 5) notable achievements and awards during the year.

Automatic Detection Of Sleep And Wake States In Mice Using Piezoelectric Sensors, Dharshan C. Medonza

University of Kentucky Master's Theses

Currently technologies such as EEG, EMG and EOG recordings are the established methods used in the analysis of sleep. But if these methods are to be employed to study sleep in rodents, extensive surgery and recovery is involved which can be both time consuming and costly. This thesis presents and analyzes a cost effective, non-invasive, high throughput system for detecting the sleep and wake patterns in mice using a piezoelectric sensor. This sensor was placed at the bottom of the mice cages to monitor the movements of the mice. The thesis work included the development of the instrumentation and signal …

Self Referencing Spr Sensor By Simultaneous Excitation Of Long And Short Range Surface Plasmon Modes, Raghunandan K. Donipudi

University of Kentucky Master's Theses

A novel surface plasmon resonance sensor is fabricated to evaluate its use in biochemical sensing. The sensor can differentiate between bulk refractive index changes and surface binding reactions of interest. There has been a great interest in developing sensors to differentiate biological or chemical agents from interfering effects, but they still remain in research phase. In this work, a prism coupler is used to simultaneously excite both long and short range surface plasmon modes of the sensor. The differing sensitivities of the long and short range modes allow one to distinguish surface binding reactions of interest from refractive index fluctuations. …

The Speciation Of Metals In Mammals Influences Their Toxicokinetics And Toxicodynamics And Therefore Human Health Risk Assessment, Robert A. Yokel, Stephen M. Lasley, David C. Dorman

Pharmaceutical Sciences Faculty Publications

Chemical form (i.e., species) can influence metal toxicokinetics and toxicodynamics and should be considered to improve human health risk assessment. Factors that influence metal speciation (and examples) include: (1) carrier-mediated processes for specific metal species (arsenic, chromium, lead and manganese), (2) valence state (arsenic, chromium, manganese and mercury), (3) particle size (lead and manganese), (4) the nature of metal binding ligands (aluminum, arsenic, chromium, lead, and manganese), (5) whether the metal is an organic versus inorganic species (arsenic, lead, and mercury), and (6) biotransformation of metal species (aluminum, arsenic, chromium, lead, manganese and mercury). The influence of speciation on metal …