Digital Commons Network^™

The Role Of Macrophages In Exercise And Insulin Resistance In Human Skeletal Muscle, Jason S. Groshong

Theses and Dissertations--Physiology

Muscle biopsies were taken at baseline, post eccentric exercise, post aerobic training, and after training followed by eccentric exercise from adults with different health status. In Cell Western analysis of pAkt/Akt ratio suggests that muscle cells isolated from baseline biopsies respond to insulin in a dose dependent manner that tracks with sensitivity to insulin of the host; however, this is uncoupled from glucose disposal in vitro. Nitrotyrosine (NY), a marker of free radical damage, was employed to assess the efficacy of the exercise paradigm. NY immunohistochemistry on muscle cross-sections revealed that eccentric exercise significantly increased damage in older ( …

Latexin’S Role In Regulating Hematopoietic Stem And Progenitor Cells, Yi Liu

Theses and Dissertations--Physiology

Previous studies in our lab identified a novel gene, latexin (Lxn), that regulates murine hematopoietic stem cells through balancing apoptosis, self-renewal and proliferation. In these dissertation studies, I performed a series of experiments to examine the function of Lxn using a Lxn conventional knockout mouse, and characterize Lxn’s role in the presence of hematopoietic stresses such as ionizing radiation, cytokines induced-mobilization, and hematopoietic malignancy.

The first series of experiments was designed to determine the role of Lxn in hematopoiesis under homeostatic conditions. I found that Lxn-/- mice exhibited hyperproliferative hematopoiesis, a repopulation advantage and elevated self-renewal capacity …

Preclinical Development Of Phytocannabinoid- And Endocannabinoid- Based Pharmacotherapies For The Treatment Of Ethanol-Induced Neurodegeneration, Daniel J. Liput

Theses and Dissertations--Pharmacy

Excessive ethanol consumption, characteristic of alcohol use disorders (AUDs), is associated with widespread neurodegeneration and cognitive and behavioral impairments that may contribute to the chronic and relapsing nature of alcoholism. Therefore, identifying novel targets that can afford neuroprotection will undoubtedly aid current treatment strategies for AUDs. The cannabinoids have been shown to provide neuroprotection in a variety of preclinical models of neurodegeneration; however minimal data is available regarding the use of cannabinoid-based pharmacotherapies for treating ethanol-induced neurodegeneration. Therefore, the current dissertation examined the overarching hypothesis: the cannabinoids are a therapeutic strategy to afford neuroprotection in the context of ethanol-induced neurodegeneration. …

Effects Of Family Structure On Educational Attainment And Health Insurance Coverage Of Youth In The Lower Mississippi Delta Region, Chaquenta L. Smith

Theses and Dissertations--Agricultural Economics

A large body of research, typically nationally focused, has examined the relationship between family structure, educational attainment, and healthcare access. Within this field of study, there is limited availability of regionally based studies, specifically the Lower Mississippi Delta (LMD) region. This exploratory study examines the effects of family structure on high school graduation rates and health insurance coverage within the LMD region. The objective is to determine if family structure has a direct impact on the educational attainment and health outcomes of a child within the region using concepts from nationally focused literature. Through the use of an OLS regression, …

Characterization And Distribution Of Novel Non-Ltr Retroelements Driving High Telomere Rflp Diversity In Clonal Lines Of Magnaporthe Oryzae, John H. Starnes

Theses and Dissertations--Plant Pathology

The filamentous ascomycete fungus Magnaporthe oryzae is a pathogen of over 50 genera of grasses. Two important diseases it can cause are gray leaf spot in Lolium perenne (perennial ryegrass) and blast in Oryza sativa (rice). The telomeres of M. oryzae isolates causing gray leaf spot are highly variable, and can spontaneously change during fungal culture. In this dissertation, it is shown that a rice-infecting isolate is much more stable at the telomeres than an isolate from gray leaf spot. To determine the molecular basis of telomere instability several gray leaf spot isolates telomeres were cloned, which revealed two non-LTR …

Blackops: Increasing Confidence In Variant Detection Through Mappability Filtering, Christopher R. Cabanski, Matthew D. Wilkerson, Matthew Soloway, Joel S. Parker, Jinze Liu, Jan F. Prins, J. S. Marron, Charles M. Perou, D. Neil Hayes

Computer Science Faculty Publications

Identifying variants using high-throughput sequencing data is currently a challenge because true biological variants can be indistinguishable from technical artifacts. One source of technical artifact results from incorrectly aligning experimentally observed sequences to their true genomic origin (‘mismapping’) and inferring differences in mismapped sequences to be true variants. We developed BlackOPs, an open-source tool that simulates experimental RNA-seq and DNA whole exome sequences derived from the reference genome, aligns these sequences by custom parameters, detects variants and outputs a blacklist of positions and alleles caused by mismapping. Blacklists contain thousands of artifact variants that are indistinguishable from true variants and, …

Diffsplice: The Genome-Wide Detection Of Differential Splicing Events With Rna-Seq, Yin Hu, Yan Huang, Ying Du, Christian F. Orellana, Darshan Singh, Amy R. Johnson, Anaïs Monroy, Pei-Fen Kuan, Scott M. Hammond, Liza M. Hammond, Scott H. Randell, Derek Y. Chiang, D. Neil Hayes, Corbin Jones, Yufeng Liu, Jan F. Prins, Jinze Liu

Computer Science Faculty Publications

The RNA transcriptome varies in response to cellular differentiation as well as environmental factors, and can be characterized by the diversity and abundance of transcript isoforms. Differential transcription analysis, the detection of differences between the transcriptomes of different cells, may improve understanding of cell differentiation and development and enable the identification of biomarkers that classify disease types. The availability of high-throughput short-read RNA sequencing technologies provides in-depth sampling of the transcriptome, making it possible to accurately detect the differences between transcriptomes. In this article, we present a new method for the detection and visualization of differential transcription. Our approach does …

Getting To The Other Side: An Exploration Of The Head And Neck Cancer Treatment Experience, Heather M. Wallace

Theses and Dissertations--Gerontology

Diagnosis of head and neck squamous cell carcinoma (HNSCC) presents a multifarious problem. Late stage diagnosis, uncertainty regarding appropriate clinical treatment, as well as the high potential for disfigurement and functional loss resulting in diminished quality of life, contributes to anxiety, stress, fear, and uncertainty throughout the cancer treatment experience. This qualitative study sought to explore the cancer treatment experience of adults with newly diagnosed HNSCC, including laryngeal, esophageal, and oral cancers. Study participants were recruited from the University of Kentucky Ear Nose and Throat Clinic in Lexington KY. Participants agreed to be interviewed after receipt of their cancer diagnosis …

The Relevance Of Martin Luther King, Jr.’S Nonviolence Philosophy To Diversity And Inclusion Efforts, Dorneshia L. Thomas

Gaines Fellow Senior Theses

No abstract provided.

Comparison Of Th1 Cytokines And T Cell Markers Gene Expressions Between Virulent And An Attenuated Eiav Vaccine Strain, Talia R. Henkle

Lewis Honors College Capstone Collection

The equine infectious anemia virus (EIAV) is closely related to HIV and has been used as a model to identify protective mechanisms against lentivirus infection. In horses, EIA infection progresses for about a year before infected horses manage to control virus replication. This naturally-gained protection is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. As the resolution of initial viremia correlates with the appearance of virus specific cytotoxic T lymphocytes (CTL), we believe that cellular immune responses play a key role in controlling EIAV in the horse. In …

Investigating Key Post-Pks Enzymes From Gilvocarcin Biosynthetic Pathway, Nidhi Tibrewal

Theses and Dissertations--Pharmacy

Gilvocarcin V (GV) belongs to the angucycline class of antibiotics that possesses remarkable anticancer and antibacterial activities with low toxicity. Gilvocarcin exhibits its light induced anticancer activity by mediating crosslinking between DNA and histone H3. When photo-activated by near-UV light, the C8 vinyl group forms a [2+2] cycloadduct with thymine residues of double stranded DNA. _D-fucofuranose is considered essential for histone H3 interactions. However, the poor water solubility has rendered it difficult to develop gilvocarcin as a drug. We aim to design novel gilvocarcin analogues with improved pharmaceutical properties through chemo-enzymatic synthesis and mutasynthesis. Previous studies have characterized many …

Comparing Clinical Profiles In Alzheimer's Disease And Parkinson's Disease Dementia, Martin R. Farlow, Frederick A. Schmitt, Dag Aarsland, George T. Grossberg, Monique Somogyi, Xiangyi Meng

Sanders-Brown Center on Aging Faculty Publications

BACKGROUND: Greater understanding of differences in baseline impairment and disease progression in patients with Alzheimer's disease (AD) and Parkinson's disease dementia (PDD) may improve the interpretation of drug effects and the design of future studies.

METHODS: This was a retrospective analysis of three randomized, double-blind rivastigmine databases (one in PDD, two in AD). Impairment on the Alzheimer's Disease Assessment Scale-cognitive subscale (ADAS-cog), Alzheimer's Disease Cooperative Study-Activities of Daily Living (ADCS-ADL) scale, 10-item Neuropsychiatric Inventory (NPI-10) and the ADCS-Clinical Global Impression of Change (CGIC) was compared [standardized difference (Cohen's d), similar if <0.1].

RESULTS: Patients with AD or PDD had similar levels …

Diagnosis Of Systemic Inflammation Using Transendothelial Electrical Resistance And Low-Temperature Co-Fired Ceramic Materials, William L. Mercke

Theses and Dissertations--Chemical and Materials Engineering

Systemic inflammation involves a complex array of cytokines that can result in organ dysfunction. Mortality remains high despite the vast amount of research conducted to find an effective biomarker. The cause of systemic inflammation can be broad and non-specific; therefore, this research investigates using transendothelial electrical resistance (TEER) measurements to better define systemic inflammatory response syndrome (SIRS)/sepsis within a patient. Results show a difference in TEER measurements between healthy individuals and SIRS-rated patients. This research also displays correlations between TEER measurements and biomarkers currently studied with systemic inflammation (tumor necrosis factor-α, C- reactive protein, procalcitonin). Furthermore, this research also presents …

Uses Of Phage Display In Agriculture: Sequence Analysis And Comparative Modeling Of Late Embryogenesis Abundant Client Proteins Suggest Protein-Nucleic Acid Binding Functionality, Rekha Kushwaha, A. Bruce Downie, Christina M. Payne

Horticulture Faculty Publications

A group of intrinsically disordered, hydrophilic proteins-Late Embryogenesis Abundant (LEA) proteins-has been linked to survival in plants and animals in periods of stress, putatively through safeguarding enzymatic function and prevention of aggregation in times of dehydration/heat. Yet despite decades of effort, the molecular-level mechanisms defining this protective function remain unknown. A recent effort to understand LEA functionality began with the unique application of phage display, wherein phage display and biopanning over recombinant Seed Maturation Protein homologs from Arabidopsis thaliana and Glycine max were used to retrieve client proteins at two different temperatures, with one intended to represent heat stress. From …

Exploring Biopsychosocial (Bps) Facets Of Chronic Obstructive Pulmonary Disease (Copd) In Patients In An Acute Inpatient Physical Rehabilitation Facility (Irf), Ronnetta Williams

Theses and Dissertations--Educational, School, and Counseling Psychology

From a BPS perspective, COPD and other chronic diseases may have a significant negative impact on those living with them and may be associated with higher rates of depression and anxiety and lower levels of health-related quality of life (HRQOL). Certain factors, such as spirituality, may influence the negative impact of chronic disease on the relationship between mood and functional independence and HRQOL. Also, gender may influence the relationship between mood, spirituality, and HRQOL for men and women living with chronic diseases. The current study included 136 patients undergoing physical rehabilitation at an IRF. Anxiety, depression, spirituality, HRQOL, and functional …

Task-Based Noise Exposures For Farmers Involved In Grain Production, Michael J. Humann, Wayne T. Sanderson, Kelley J. Donham, Kevin M. Kelly

Epidemiology and Environmental Health Faculty Publications

Few studies have been done examining noise exposures associated with agricultural tasks. This study was conducted to address that research gap by calculating the noise exposures for tasks and equipment associated with grain production and assessing the variability in those exposures. An additional aim of this study was to identify tasks and equipment that could be targeted for intervention strategies as a means toward reducing the total noise exposures of farmers and farm workers. Through the use of personal noise dosimetry and direct observation, over 30,000 one-minute noise exposure measurements and corresponding task and equipment data were collected on 18 …

Memory, Cognition, And The Effect Of A Music Intervention On Healthy Older Adults, Shannon L. Bowles

Theses and Dissertations--Gerontology

Music is a powerful modality that can bring about changes in individuals of all ages. This research employed both an experimental and quasi-experimental design to identify the effects of music as it influenced psychological well-being, memory, and cognition among older adults. Specifically, it addressed three aims: (a) To determine to what extent learning to play a music instrument later in life influenced psychological well-being and cognitive function of non-institutionalized healthy seniors, (b) To determine the effects of the amount of music involvement on psychological well-being and cognitive function (c) To determine the benefit of music for those with limited/no music …

Global Kentucky (Winter 2013), University Of Kentucky International Center

Global Kentucky

No abstract provided.

Compost Bedded Pack Barns: Management Practices And Economic Implications, Randi Alyson Black

Theses and Dissertations--Animal and Food Sciences

Compost bedded pack (CBP) barn design and pack maintenance procedures vary considerably, making advising and problem-solving challenging. One objective of this research was to characterize herd performance and management practices employed by Kentucky CBP managers (42 farms and 47 CBP facilities). Producer satisfaction, changes in historical bulk-tank somatic cell count, and improvement in herd performance parameters after transitioning to a CBP barn support reported CBP barn system benefits. Daily milk production increased from before moving into the CBP barn to the second year after (29.3 ± 0.3 vs. 30.7 ± 0.3 kg, respectively; P < 0.05) for farms using the CBP barn as the primary housing facility (n = 8). Increasing stirring frequency, stirring depth, and ambient temperatures increased pack temperature. Increased drying rate decreased CBP moisture. Increased 20.3 cm depth CBP temperature and ambient temperatures improved cow hygiene. Mastitis-causing bacteria thrive in conditions similar to optimal composting bacteria conditions, making reduction of these bacteria difficult in an active composting environment. Producers must pay attention to other management areas where preventive measures can be employed. The New Dairy Housing Investment Analysis Dashboard provides users an interactive and flexible decision tool to make more informed facility investment decisions.

Cooperative Semantic Information Processing For Literature-Based Biomedical Knowledge Discovery, Zhiguo Yu

Theses and Dissertations--Electrical and Computer Engineering

Given that data is increasing exponentially everyday, extracting and understanding the information, themes and relationships from large collections of documents is more and more important to researchers in many areas. In this paper, we present a cooperative semantic information processing system to help biomedical researchers understand and discover knowledge in large numbers of titles and abstracts from PubMed query results.

Our system is based on a prevalent technique, topic modeling, which is an unsupervised machine learning approach for discovering the set of semantic themes in a large set of documents. In addition, we apply a natural language processing technique to …

Heterogeneity In Platelet Exocytosis, Deepa Jonnalagadda

Theses and Dissertations--Molecular and Cellular Biochemistry

Platelet exocytosis is essential for hemostasis and for many of its sequelae. Platelets release numerous bioactive molecules stored in their granules enabling them to exert a wide range of effects on the vascular microenvironment. Are these granule cargo released thematically in a context-specific pattern or via a stochastic, kinetically-controlled process? My work describes platelet exocytosis using a systematic examination of platelet secretion kinetics. Platelets were stimulated for increasing times with different agonists (i.e. thrombin, PAR1-agonist, PAR4-agonist, and convulxin) and micro-ELISA arrays were used to quantify the release of 28 distinct α-granule cargo molecules. Agonist potency directly correlated with the speed …

Investigating Therapeutic Options For Lafora Disease Using Structural Biology And Translational Methods, Amanda R. Sherwood

Theses and Dissertations--Molecular and Cellular Biochemistry

Lafora disease (LD) is a rare yet invariably fatal form of epilepsy characterized by progressive degeneration of the central nervous and motor systems and accumulation of insoluble glucans within cells. LD results from mutation of either the phosphatase laforin, an enzyme that dephosphorylates cellular glycogen, or the E3 ubiquitin ligase malin, the binding partner of laforin. Currently, there are no therapeutic options for LD, or reported methods by which the specific activity of glucan phosphatases such as laforin can be easily measured. To facilitate our translational studies, we developed an assay with which the glucan phosphatase activity of laforin as …

Increases In Rops Pricing From 2006-2012 And The Impact On Rops Demand, Julie A. Sorensen, Paul L. Jenkins, Barbara Bayes, Erin Madden, Mark Purschwitz, John J. May

Biosystems and Agricultural Engineering Faculty Publications

In 2006, a social marketing campaign was developed to increase the installation of rollover protective structures (ROPS) on unprotected New York tractors. Using data gathered from the program's hotline, the impact of price increases on farmers' interest in ROPS is examined. Pricing data were obtained for all rigid ROPS kits commercially available in the U.S. since 2006. These data were stratified into two groups of ROPS suppliers: (1) tractor manufacturers that sell ROPS for their own tractors, referred to in this study as original equipment manufacturers (OEMs), and (2) aftermarket (AM) ROPS suppliers. The trend in price increases was contrasted …

Development Of A Semi-Automated Tobacco Stripping Machine Utilizing String Trimmers, Robert George Sperry, John H. Wilhoit, George A. Duncan

Biosystems and Agricultural Engineering Faculty Publications

Conventional stripping of burley tobacco is labor intensive and typically requires 50 to 75 worker hours per acre (wkr•h/acre). The goal of the project was to reduce labor by optimizing leaf removal by string trimmer heads using combinations of strings lengths and motor speeds. In tests conducted on a single grade, all leaves outside the grade were removed by hand. Plants were run through the machine for string lengths of 13, 18, and 23 cm (5, 7, and 9 in.) and associated motor speeds which were monitored and recorded. Stripping efficiencies were calculated for each plant and collectively for each …

Designing Contour Weep Berms To Reduce Agricultural Nonpoint Source Pollution, Richard C. Warner, Carmen T. Agouridis, Ross L. Guffey

Biosystems and Agricultural Engineering Faculty Publications

Nonpoint source pollution (NPS) of surface waters is a significant issue in agricultural lands, and best management practices (BMPs) are often used to reduce these impacts. Since the effectiveness of a BMP depends on a large number of widely varying factors, it is important to continue to develop BMPs in order to provide designers with more tools to use to maximize NPS removal. The contour weep berm is a new structural BMP constructed out of earth and subsequently vegetated. It is a linear BMP that is used in combination with a down-gradient vegetated filter strip or forested riparian buffer. Preliminary …

Influence Of Flue Gas Components On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus, Czarena Crofcheck, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to the increase in combustion of fossil fuels. One possible alternative strategy is the use of microalgae for CO₂ capture and recycling. Major components in coal-derived flue gas that may accumulate and effect algae growth include both sulfur oxides and fly ash. However, in practical application, sulfur oxides will be converted quickly to the acid product (H₂SO₄) in the aerobic aqueous conditions of algae cultivation. In this article, the influence of elevated H₂SO₄ levels and the presence of coal-derived fly ash …

A Correlation-Based Optical Flowmeter For Enclosed Flows, Naiqian Zhang, Joseph S. Dvorak, Yali Zhang

Biosystems and Agricultural Engineering Faculty Publications

A low-cost flowmeter would be very useful in a wide variety of monitoring situations. This article discusses the development of such a flowmeter based on optical components and its testing with water in an enclosed flow system. The sensor consisted of two sets of LEDs and phototransistors spaced 4 cm apart, monitoring the optical properties of the fluid at upstream and downstream locations, respectively. A small amount of dye was injected into the flow, which caused a change in the optical properties of the fluid at both locations. The time required for this change to move from the upstream to …

Mirror, Mirror In The Stall: Animal Cloning And Its Legal Implications For Equine Registry Associations, Mary W. Craig

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Multifactorial Modulation Of The Blood-Brain Barrier: Relationship To Stroke, Bei Zhang

Theses and Dissertations--Nutritional Sciences

The blood-brain barrier (BBB) is a dynamic interface, mainly consisting of highly specialized brain microvascular endothelial cells (BMECs) that segregate the central nervous system (CNS) from the peripheral circulation. Impairment of the BBB, due to disruption of tight junction (TJ) proteins and inflammatory responses, may initiate and/or contribute to the progress of CNS disorders, including stroke. Stroke is the second leading cause of death worldwide. It has been shown that aging and environmental pollutants can induce brain endothelium dysfunction, and are considered as risk factors for stroke.

Deficiency of telomerase is highly linked with aging-associated vascular diseases. Evidence indicates that …

Polychlorinated Biphenyl Ligands Of The Aryl Hydrocarbon Receptor Promote Adipocyte-Mediated Diabetes, Nicki A. Baker

Theses and Dissertations--Nutritional Sciences

Numerous epidemiology studies suggest a correlation between exposures to polychlorinated biphenyls (PCBs) and the development and severity of type 2 diabetes (T2D); however, mechanisms remain largely unknown. Previous studies demonstrated that PCBs that are ligands of the aryl hydrocarbon receptor (AhR) promote the expression of proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), that are linked to insulin resistance in adipocytes. To explore potential mechanisms linking PCB exposures to diabetes, we developed a mouse model of glucose and insulin intolerance induced by acute and chronic exposures to PCB-77. We hypothesized that PCB ligands of AhR result in adipocyte-specific elevations in TNF-α …