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Crystallization And Structure Of A Plant Peptide Deformylase, David W. Rodgers, Robert L. Houtz, Lynnette M. A. Dirk, Mark Williams

Horticulture Faculty Patents

This invention relates to the crystal structure of a plant peptide deformylase polypeptide and methods of using the structure to design compounds that modulate the activity of the polypeptide.

Salamander Hox Clusters Contain Repetitive Dna And Expanded Non-Coding Regions: A Typical Hox Structure For Non-Mammalian Tetrapod Vertebrates?, Stephen Randal Voss, Srikrishna Putta, John A. Walker, Jeramiah J. Smith, Nobuyasu Maki, Panagiotis A. Tsonis

Biology Faculty Publications

Hox genes encode transcription factors that regulate embryonic and post-embryonic developmental processes. The expression of Hox genes is regulated in part by the tight, spatial arrangement of conserved coding and non-coding sequences. The potential for evolutionary changes in Hox cluster structure is thought to be low among vertebrates; however, recent studies of a few non-mammalian taxa suggest greater variation than originally thought. Using next generation sequencing of large genomic fragments (>100 kb) from the red spotted newt (Notophthalamus viridescens), we found that the arrangement of Hox cluster genes was conserved relative to orthologous regions from other vertebrates, but the …

Svsxp: A Strongylus Vulgaris Antigen With Potential For Prepatent Diagnosis, Ulla V. Andersen, Daniel K. Howe, Sriveny Dangoudoubiyam, Nils Toft, Craig R. Reinemeyer, Eugene T. Lyons, Susanne N. Olsen, Jesper Monrad, Peter Nejsum, Martin K. Nielsen

Veterinary Science Faculty Publications

BACKGROUND: Strongyle parasites are ubiquitous in grazing horses. Strongylus vulgaris, the most pathogenic of the large strongyles, is known for its extensive migration in the mesenteric arterial system. The lifecycle of S. vulgaris is characterised by a long prepatent period where the migrating larvae are virtually undetectable as there currently is no test available for diagnosing prepatent S. vulgaris infection. Presence of S. vulgaris larvae in the arterial system causes endarteritis and thrombosis with a risk of non-strangulating intestinal infarctions. Emergence of anthelmintic resistance among cyathostomins has led to recommendations of reduced treatment intensity by targeting horses that exceed a …

The P38Α Mapk Regulates Microglial Responsiveness To Diffuse Traumatic Brain Injury, Adam D. Bachstetter, Rachel K. Rowe, Machi Kaneko, Danielle Goulding, Jonathan Lifshitz, Linda J Van Eldik

Sanders-Brown Center on Aging Faculty Publications

Neuropathology after traumatic brain injury (TBI) is the result of both the immediate impact injury and secondary injury mechanisms. Unresolved post-traumatic glial activation is a secondary injury mechanism that contributes to a chronic state of neuroinflammation in both animal models of TBI and human head injury patients. We recently demonstrated, using in vitro models, that p38α MAPK signaling in microglia is a key event in promoting cytokine production in response to diverse disease-relevant stressors and subsequent inflammatory neuronal dysfunction. From these findings, we hypothesized that the p38α signaling pathway in microglia could be contributing to the secondary neuropathologic sequelae after …

Thermodynamics Of Binding By Calmodulin Correlates With Target Peptide Α-Helical Propensity, Tori B. Dunlap, Jessime M. Kirk, Emily A. Pena, Meghan S. Yoder, Trevor P. Creamer

Center for Structural Biology Faculty Publications

In this work, we have examined contributions to the thermodynamics of calmodulin (CaM) binding from the intrinsic propensity for target peptides to adopt an α-helical conformation. CaM target sequences are thought to commonly reside in disordered regions within proteins. Using the ability of TFE to induce α-helical structure as a proxy, the six peptides studied range from having almost no propensity to adopt α-helical structure through to a very high propensity. This despite all six peptides having similar CaM-binding affinities. Our data indicate there is some correlation between the deduced propensities and the thermodynamics of CaM binding. This finding implies …

Editor’S Preface And Acknowledgments, Richard Parmer

disClosure: A Journal of Social Theory

No abstract provided.

Security Of The Nation: Why Do We Need ‘Mothers Of Martyrs’ In Turkey?, Esra Gedik

disClosure: A Journal of Social Theory

No abstract provided.

Climate Change Extension: Presenting The Science Is Necessary But Insufficient, Paul C. Vincelli, Rebecca L. Mcculley

Plant Pathology Presentations

Why Should We Consider How to Present Scientific Information?

To engage a wide spectrum of agricultural producers in the discussion of human-induced climate change and its mitigation.

What Did We Do?

Our initial Extension efforts on climate change in Kentucky were based on an information-deficit model, which assumes that citizens fail to accept climate change because they don’t understand the science. However, social science research indicates that this topic has cultural significance for many agricultural producers, suggesting that presentation of sound scientific information alone is likely to be unpersuasive. Based on social science research, we redesigned our outreach efforts to …

Effects Of Climate Change On Pasture Production And Forage Quality, Rebecca L. Mcculley, Jim A. Nelson, A. Elizabeth Carlisle

Plant and Soil Sciences Presentations

Why Study Climate Change and Pastures?

Pastures cover more than 14 million hectares in the eastern half of the United States and support grazing animal and hay production while also contributing to the maintenance of overall environmental quality and ecosystem services. Climate change is likely to alter the function of these ecosystems. This manipulative field experiment evaluated the effect of warming and additional precipitation on forage production and quality.

What Did We Do?

We initiated a multi-factor climate change study, elevating air temperature (+3º C) and increasing growing season precipitation (+30% of long-term mean annual), in a central Kentucky pasture …

Biodistribution And Biopersistence Of Ceria Engineered Nanomaterials: Size Dependence, Robert A. Yokel, Michael T. Tseng, Mo Dan, Jason M. Unrine, Uschi M. Graham, Peng Wu, Eric A. Grulke

Pharmaceutical Sciences Faculty Publications

The aims were to determine the biodistribution, translocation, and persistence of nanoceria in the brain and selected peripheral organs. Nanoceria is being studied as an anti-oxidant therapeutic. Five, 15, 30, or 55 nm ceria was iv infused into rats which were terminated 1, 20, or 720 h later. Cerium was determined in blood, brain, liver, and spleen. Liver and spleen contained a large percentage of the dose, from which there was no significant clearance over 720 h, associated with adverse changes. Very little nanoceria entered brain parenchyma. The results suggest brain delivery of nanoceria will be a challenge.

FROM THE …

Nutrition Intervention To Decrease Symptoms In Patients With Advanced Heart Failure, Terry A. Lennie, Debra K. Moser, Martha J. Biddle, Darlene Welsh, Geza G. Bruckner, D. Travis Thomas, Mary Kay Rayens, Alison L. Bailey

Nursing Faculty Publications

For a majority of patients with advanced heart failure, there is a need for complementary, non-pharmacologic interventions that could be easily implemented by health care providers to provide palliative care. Three major pathologic pathways underlying heart failure symptoms have been identified: fluid overload, inflammation, and oxidative stress. Prior research has demonstrated that three nutrients-sodium, omega-3 fatty acids, and lycopene-can alter these pathologic pathways. Therefore, the purposes of this study are to test the effects of a 6-month nutrition intervention of dietary sodium reduction combined with supplementation of lycopene and omega-3 fatty acids on heart failure symptoms, health-related quality of life, …

Derivation Of Multivariate Syndromic Outcome Metrics For Consistent Testing Across Multiple Models Of Cervical Spinal Cord Injury In Rats, Adam R. Ferguson, Karen-Amanda Irvine, John C. Gensel, Jessica L. Nielson, Amity Lin, Johnathan Ly, Mark R. Segal, Rajiv R. Ratan, Jacqueline C. Bresnahan, Michael S. Beattie

Physiology Faculty Publications

Spinal cord injury (SCI) and other neurological disorders involve complex biological and functional changes. Well-characterized preclinical models provide a powerful tool for understanding mechanisms of disease; however managing information produced by experimental models represents a significant challenge for translating findings across research projects and presents a substantial hurdle for translation of novel therapies to humans. In the present work we demonstrate a novel 'syndromic' information-processing approach for capitalizing on heterogeneous data from diverse preclinical models of SCI to discover translational outcomes for therapeutic testing. We first built a large, detailed repository of preclinical outcome data from 10 years of basic …

Corpse Management In Social Insects, Qian Sun, Xuguo Zhou

Entomology Faculty Publications

Undertaking behavior is an essential adaptation to social life that is critical for colony hygiene in enclosed nests. Social insects dispose of dead individuals in various fashions to prevent further contact between corpses and living members in a colony. Focusing on three groups of eusocial insects (bees, ants, and termites) in two phylogenetically distant orders (Hymenoptera and Isoptera), we review mechanisms of death recognition, convergent and divergent behavioral responses toward dead individuals, and undertaking task allocation from the perspective of division of labor. Distinctly different solutions (e.g., corpse removal, burial and cannibalism) have evolved, independently, in the holometabolous hymenopterans and …

High-Activity Mutants Of Butyrylcholinesterase For Cocaine Hydrolysis And Method Of Generating The Same, Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai

Pharmaceutical Sciences Faculty Patents

A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• The New Kentucky Nitrogen and Phosphorus Risk Assessment Tool to Protect Water Quality, Tibor Horvath and others, USDA-NRCS, Lexington, KY
• Groundwater Phosphorus in Kentucky Relative to Karst, Groundwater Sensitivity, and Groundwater Physiographic Region, Caroline Chan, Kentucky Division of Water, Frankfort, KY
• Comparison of Irrigation Scheduling Based on Daily Water Use or Plant Water Demand of Container Grown Nursery Plants, Susmitha Nambuthiri and others, Dept of Horticulture, UK
• Arsenic Species in Broiler (Gallus gallus domesticus) Litter, Soils, Maize (Zea mays L.), and Groundwater from Litter-Amended Fields, Georgia Zeigler and others, Dept Plant and Soil Science, UK
• Cyberinfrastructure …

Session 2d, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• A Stability Index for Northern Kentucky Streams, Bob Hawley and others, Sustainable Streams, LLC, Louisville, KY
• The Gunpowder and Woolper Creek Watershed Initiatives, Local Cases of a National Problem, Mark Jacobs and others, Boone County Conservation District, Burlington, KY
• Watershed-Scale Model of Carbon and Nitrogen Cycles in Streams, William Ford and Jimmy Fox, Dept of Civil Engineering, UK
• Sensor Network for Suspended Sediment Monitoring, Robert Stewart and others, Dept of Civil Engineering, UK

Session 2c, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Mercury Bioaccumulation and the Impact of Stream Basin Characteristics on Susceptible Populations: Policy Implications for Kentucky, Caroline Chan, Kentucky Division of Water, Frankfort, KY
• Asian Carp in Kentucky Waters: An Ecological Disaster? Ron Brooks, Kentucky Dept of Fish and Wildlife Resources, Frankfort, KY
• Effects of Streambed Sediments on the Fate of Selenium in Eastern Kentucky Watersheds Contaminated with Surface Coal Mining Operations, Edward Fisher and Yi-Tin Wang, Dept of Civil Engineering, UK
• Thermal Dependence of Solvation Geometries in Aqueous Nitrate Ion Solutions, Matthew Nee, Dept of Chemistry, WKU, Bowling Green, KY
• The Combined Effects of Atrazine and …

Session 2b, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Developing a Vegetation-Based Index of Biotic Integrity for Assessing the Ecological Condition of Wetlands in Kentucky, Tanner Morris and others, Dept of Biological Sciences, EKU, Richmond, KY
• Using Vegetation and Landscape Analysis to Validate a Wetland Rapid Assessment Method for Kentucky's Forested Riparian Wetlands, JohnRyan Polascik and others, Dept of Biological Sciences, EKU, Richmond, KY
• Improving Overall Stream Function Beyond the Channel (Opportunity Meets Necessity), Oakes Routt and Wanda Lawson, Stantec Consulting Services Inc., Louisville, KY
• The Effects of Prescribed Fire Restoration on Amphibian and Reptile Diversity, Robert Knopp and others, Murray State University, Murray, KY …

Session 1c, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Engaging Partners in the Cane Rune Watershed, Carol Hanley and others, College of Agriculture, UK
• Water Quality Monitoring Results from the McConnell Springs Stormwater Quality Wetland Pond and Gainseway Pond Retrofit Project, David Price and Susan Plueger, Lexington-Fayette County Government, Lexington, KY
• Hydrologic Characterization of a Tree- and Shrub-Vegetated Rain Garden in Central Kentucky, John McMaine and Carmen Agouridis, Dept Biosystems and Agricultural Engineering, UK
• Watershed Based Planning in the Urban Wolf Run Watershed, Steven Evans, Third Rock Consultants, LLC, Lexington, KY
• Estimating Load Reduction Costs for Assessing Stormwater Best Management Practice (BMP) Feasibility: A Case …

Session 1a, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Development and Testing of a New Phosphorus Index for Kentucky, Carl Bolster and others, USDA-ARS, Bowling Green, KY
• The New Kentucky Nitrogen and Phosphorus Risk Assessment Tool to Protect Water Quality, Tibor Horvath and others, USDA-NRCS, Lexington, KY
• Herbicide Transport Within Shallow Karst Groundwater on Kentucky's Pennyroyal Plateau Beneath Row Crop Agriculture, Chris Groves and others, Hoffman Environmental Research Institute, WKU, Bowling Green, KY
• Groundwater Tracing in the West Pennyrile Karst Region, Robert Blair and Jessica Moore, Kentucky Division of Water, Frankfort, KY

Loss Of Fbp1 By Snail-Mediated Repression Provides Metabolic Advantages In Basal-Like Breast Cancer, Chenfang Dong, Tingting Yuan, Yadi Wu, Yifan Wang, Teresa W-M Fan, Sumitra Miriyala, Yiwei Lin, Jun Yao, Jian Shi, Tiebang Kang, Pawel Lorkiewicz, Daret St. Clair, Mien-Chie Hung, B. Mark Evers, Binhua P. Zhou

Toxicology and Cancer Biology Faculty Publications

The epithelial-mesenchymal transition (EMT) enhances cancer invasiveness and confers tumor cells with cancer stem cell (CSC)-like characteristics. We show that the Snail-G9a-Dnmt1 complex, which is critical for E-cadherin promoter silencing, is also required for the promoter methylation of fructose-1,6-biphosphatase (FBP1) in basal-like breast cancer (BLBC). Loss of FBP1 induces glycolysis and results in increased glucose uptake, macromolecule biosynthesis, formation of tetrameric PKM2, and maintenance of ATP production under hypoxia. Loss of FBP1 also inhibits oxygen consumption and reactive oxygen species production by suppressing mitochondrial complex I activity; this metabolic reprogramming results in an increased CSC-like property and tumorigenicity by enhancing …

Sensory Systems And Environmental Change On Behavior During Social Interactions, Sonya M. Bierbower, J. Nadolski, Robin L. Cooper

Biology Faculty Publications

The impact of environmental conditions for transmitting sensory cues and the ability of crayfish to utilize olfaction and vision were examined in regards to social interactive behavior. The duration and intensity of interactions were examined for conspecific crayfish with different sensory abilities. Normally, vision and chemosensory have roles in agonistic communication of Procambarus clarkii; however, for the blind cave crayfish (Orconectes australis packardi), that lack visual capabilities, olfaction is assumed to be the primary sensory modality. To test this, we paired conspecifics in water and out of water in the presence and absence of white light to …

Bed Bugs Evolved Unique Adaptive Strategy To Resist Pyrethroid Insecticides, Fang Zhu, Hemant Gujar, Jennifer R. Gordon, Kenneth F. Haynes, Michael F. Potter, Subba R. Palli

Entomology Faculty Publications

Recent advances in genomic and post-genomic technologies have facilitated a genome-wide analysis of the insecticide resistance-associated genes in insects. Through bed bug, Cimex lectularius transcriptome analysis, we identified 14 molecular markers associated with pyrethroid resistance. Our studies revealed that most of the resistance-associated genes functioning in diverse mechanisms are expressed in the epidermal layer of the integument, which could prevent or slow down the toxin from reaching the target sites on nerve cells, where an additional layer of resistance (kdr) is possible. This strategy evolved in bed bugs is based on their unique morphological, physiological and behavioral characteristics …

Integrated Analysis Of Cytochrome P450 Gene Superfamily In The Red Flour Beetle, Tribolium Castaneum, Fang Zhu, Timothy W. Moural, Kapil Shah, Subba Reddy Palli

Entomology Faculty Publications

BACKGROUND: The functional and evolutionary diversification of insect cytochrome P450s (CYPs) shaped the success of insects. CYPs constitute one of the largest and oldest gene superfamilies that are found in virtually all aerobic organisms. Because of the availability of whole genome sequence and well functioning RNA interference (RNAi), the red flour beetle, Tribolium castaneum serves as an ideal insect model for conducting functional genomics studies. Although several T. castaneum CYPs had been functionally investigated in our previous studies, the roles of the majority of CYPs remain largely unknown. Here, we comprehensively analyzed the phylogenetic relationship of all T. castaneum CYPs …

Three-Dimensional Structure Of Victorivirus Hvv190s Suggests Coat Proteins In Most Totiviruses Share A Conserved Core, Sarah E. Dunn, Hua Li, Giovanni Cardone, Max L. Nibert, Said A. Ghabrial, Timothy S. Baker

Plant Pathology Faculty Publications

Double-stranded (ds)RNA fungal viruses are currently assigned to six different families. Those from the family Totiviridae are characterized by nonsegmented genomes and single-layer capsids, 300-450 Å in diameter. Helminthosporium victoriae virus 190S (HvV190S), prototype of recently recognized genus Victorivirus, infects the filamentous fungus Helminthosporium victoriae (telomorph: Cochliobolus victoriae), which is the causal agent of Victoria blight of oats. The HvV190S genome is 5179 bp long and encompasses two large, slightly overlapping open reading frames that encode the coat protein (CP, 772 aa) and the RNA-dependent RNA polymerase (RdRp, 835 aa). To our present knowledge, victoriviruses uniquely express their RdRps via …

Two Biochemically Distinct Lipophosphoglycans From Leishmania Braziliensis And Leishmania Infantum Trigger Different Innate Immune Responses In Murine Macrophages, Izabela Coimbra Ibraim, Rafael Ramiro De Assis, Natália Lima Pessoa, Marco Antônio Campos, Maria Norma Melo, Salvatore Joseph Turco, Rodrigo Pedro Soares

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: The dominant, cell surface lipophosphoglycan (LPG) of Leishmania is a multifunctional molecule involved in the interaction with vertebrate and invertebrate hosts. Although the role of LPG on infection has been extensively studied, it is not known if LPG interspecies variations contribute to the different immunopathologies of leishmaniases. To investigate the issue of interspecies polymorphisms, two Leishmania species from the New World that express structural variations of side chains of LPG repeat units were examined. In this context, the procyclic form of L. braziliensis LPG (strain M2903), is devoid of side chains, while the L. infantum LPG (strain BH46) has …

Effects Of Aging On Apoptosis Gene Expression In Oral Mucosal Tissues, Octavio A. Gonzalez, Michael John Novak, Sreenatha S. Kirakodu, Arnold J. Stromberg, Shu Shen, Luis Orraca, Janis Gonzalez-Martinez, Jeffrey L. Ebersole

Center for Oral Health Research Faculty Publications

Apoptotic processes are important for physiologic renewal of an intact epithelial barrier and contribute some antimicrobial resistance for bacteria and viruses, as well as anti-inflammatory effects that benefits the mucosa. The oral cavity presents a model of host-bacterial interactions at mucosal surfaces, in which a panoply of microorganisms colonizes various niches in the oral cavity and creates complex multispecies biofilms that challenge the gingival tissues. This report details gene expression in apoptotic pathways that occur in oral mucosal tissues across the lifespan, using a nonhuman primate model. Macaca mulatta primates from 2 to 23 years of age (n = …

Physicochemical Characterization And Aerosol Dispersion Performance Of Organic Solution Advanced Spray-Dried Cyclosporine A Multifunctional Particles For Dry Powder Inhalation Aerosol Delivery, Xiao Wu, Weifen Zhang, Don Hayes Jr., Heidi M. Mansour

Pharmaceutical Sciences Faculty Publications

In this systematic and comprehensive study, inhalation powders of the polypeptide immunosuppressant drug - cyclosporine A - for lung delivery as dry powder inhalers (DPIs) were successfully designed, developed, and optimized. Several spray drying pump rates were rationally chosen. Comprehensive physicochemical characterization and imaging was carried out using scanning electron microscopy, hot-stage microscopy, differential scanning calorimetry, powder X-ray diffraction, Karl Fischer titration, laser size diffraction, and gravimetric vapor sorption. Aerosol dispersion performance was conducted using a next generation impactor with a Food and Drug Administration-approved DPI device. These DPIs displayed excellent aerosol dispersion performance with high values in emitted dose, …

Population Impacts Of Wolbachia On Aedes Albopictus, James W. Mains, Corey L. Brelsfoard, Philip R. Crain, Yunxin Huang, Stephen L. Dobson

Entomology Faculty Publications

Prior studies have demonstrated that Wolbachia, a commonly occurring bacterium capable of manipulating host reproduction, can affect life history traits in insect hosts, which in turn can have population-level effects. Effects on hosts at the individual level are predicted to impact population dynamics, but the latter has not been examined empirically. Here, we describe a biological model system based on Aedes albopictus (Asian tiger mosquito) that allows for measurement of population dynamics, which has not been accomplished in prior field trials or laboratory designs. The results demonstrate the studied populations to be robust and allow for persistent, closed populations with …

Excitatory Amino Acids Display Compartmental Disparity Between Plasma And Synovial Fluid In Clinical Arthropathies, Terry A. Mcnearney, Karin N. Westlund

Physiology Faculty Publications

BACKGROUND: Previous studies have demonstrated elevated levels of excitatory amino acids (EAA) glutamate (Glu) and aspartate (Asp) in the synovial fluid (SF) of patients with active arthritis. The source of SF EAA concentrations are thought in large part to be secondary to passive diffusion from the plasma across synovial membranes and less so, reflective of local synovial pathology.

OBJECTIVE: This descriptive report assesses the hypothesis that the SF EAA levels reflect inflammatory processes of the joint and are not dependent on plasma levels.

METHODS: Simultaneously drawn plasma and SF samples were obtained from 14 recently deceased cadavers and 10 patients …