Digital Commons Network^™

From Citizenship To Custody: Unwed Fathers Abroad And At Home, Albertina Antognini

Law Faculty Scholarly Articles

The sex-based distinctions of the Immigration and Nationality Act (INA) have been remarkably resilient in the face of numerous equal protection challenges. In Miller v. Albright, Nguyen v. INS, and most recently United States v. Flores-Villar — collectively the "citizenship transmission cases" — the Supreme Court has upheld the constitutionality of the INA’s provisions that require unwed fathers, but not unwed mothers, to take a series of affirmative steps in order to transmit citizenship to their children born abroad.

The conventional account of these citizenship transmission cases is that the Court upholds sex-based distinctions that would otherwise fail …

Animals Represent The Past And The Future, Thomas R. Zentall

Psychology Faculty Publications

It has been proposed by some that only humans have the ability to mentallytravel back in time (i.e., have episodic memory) and forward in time (i.e., have the ability to simulate the future). However, there is evidence from a variety of nonhuman animals (e.g., primates, dolphins, scrub jays, rats, and pigeons) that they have some ability to recover personal memories of what-where-when an event occurred (an earlier requirement of the ability to recover an episodic memory) and answer unexpected questions (another requirement to distinguish between semantic and episodic memory). Also, perhaps more critically, according to Tulving’s more recent definition of …

Gene Transfer Of Mutant Mouse Cholinesterase Provides High Lifetime Expression And Reduced Cocaine Responses With No Evident Toxicity, Liyi Geng, Yang Gao, Xiabin Chen, Shurong Hou, Chang-Guo Zhan, Zoran Radic, Robin J. Parks, Stephen J. Russell, Linh Pham, Stephen Brimijoin

Pharmaceutical Sciences Faculty Publications

Gene transfer of a human cocaine hydrolase (hCocH) derived from butyrylcholinesterase (BChE) by 5 mutations (A199S/F227A/S287G/A328W/Y332G) has shown promise in animal studies for treatment of cocaine addiction. To predict the physiological fate and immunogenicity of this enzyme in humans, a comparable enzyme was created and tested in a conspecific host. Thus, similar mutations (A199S/S227A/S287G/A328W/Y332G) were introduced into mouse BChE to obtain a mouse CocH (mCocH). The cDNA was incorporated into viral vectors based on: a) serotype-5 helper-dependent adenovirus (hdAD) with ApoE promoter, and b) serotype-8 adeno-associated virus with CMV promoter (AAV-CMV) or multiple promoter and enhancer elements (AAV-VIP). Experiments on …

Does It Have To Taste Bad To Be Good? Leveraging Pleasure To Enhance Learning, Karen H. Dougherty M.D.

Kentucky Journal of Higher Education Policy and Practice

Emerging information from the field of neuroscience has illuminated the workings of the brain’s pleasure circuit as a powerful motivator of human behavior. While much of the research has been driven by an effort to uncover the roots of addiction, an understanding of the aspects of pleasure can be applied to the design of teaching strategies to engage college students and improve their retention and persistence.

Development Of Novel In Vivo Chemical Probes To Address Cns Protein Kinase Involvement In Synaptic Dysfunction, D Martin Watterson, Valerie L. Grum-Tokars, Saktimayee M. Roy, James P. Schavocky, Brinda Desai Bradaric, Adam D. Bachstetter, Bin Xing, Edgardo Dimayuga, Faisal Saeed, Hong Zhang, Agnieszka Staniszewski, Jeffrey C. Pelletier, George Minasov, Wayne F. Anderson, Ottavio Arancio, Linda J. Van Eldik

Sanders-Brown Center on Aging Faculty Publications

Serine-threonine protein kinases are critical to CNS function, yet there is a dearth of highly selective, CNS-active kinase inhibitors for in vivo investigations. Further, prevailing assumptions raise concerns about whether single kinase inhibitors can show in vivo efficacy for CNS pathologies, and debates over viable approaches to the development of safe and efficacious kinase inhibitors are unsettled. It is critical, therefore, that these scientific challenges be addressed in order to test hypotheses about protein kinases in neuropathology progression and the potential for in vivo modulation of their catalytic activity. Identification of molecular targets whose in vivo modulation can attenuate synaptic …

Use Of Parthenolide Derivatives As Antileukemic And Cytotoxic Agents, Peter A. Crooks, Craig T. Jordan, Xiaochen Wei

Pharmaceutical Sciences Faculty Patents

To view this abstract, please download this patent.

Voluntary Exercise Protects Against Methamphetamine-Induced Oxidative Stress In Brain Microvasculature And Disruption Of The Blood--Brain Barrier, Michal Toborek, Melissa J. Seelbach, Cetewayo S. Rashid, Ibolya E. András, Lei Chen, Minseon Park, Karyn A. Esser

Physiology Faculty Publications

BACKGROUND: There is no effective therapeutic intervention developed targeting cerebrovascular toxicity of drugs of abuse, including methamphetamine (METH). We hypothesize that exercise protects against METH-induced disruption of the blood--brain barrier (BBB) by enhancing the antioxidant capacity of cerebral microvessels and modulating caveolae-associated signaling. Mice were subjected to voluntary wheel running for 5 weeks resembling the voluntary pattern of human exercise, followed by injection with METH (10 mg/kg). The frequency, duration, and intensity of each running session were monitored for each mouse via a direct data link to a computer and the running data are analyzed by Clock labTM Analysis software. …

Using A “Train The Trainer” Model And Active Learning To Reach Biology Freshmen, Valerie Perry, Jason Keinsley, Renae Newhouse, Patricia Hartman, Meghan Moran

Library Presentations

In Spring 2013, we collaborated with the UK Biology Department to create, teach, and assess course-integrated information literacy sessions for 17 BIO 155 sections, reaching 462 students once a week for two weeks (thus 34 sessions reaching 934 attendees). We experimented with a “train-the-trainer” model, providing an introductory session to TAs, then alternating librarian-led sections and TA-led sections. We taught in a Macbook lab, using mini presentations mixed with active instruction and tools such as PollEverywhere and Course Guides. We assessed the information literacy concepts learned using a Google Docs survey. We will share our challenges and successes initiating this …

Functional Integration Of The Conserved Domains Of Shoc2 Scaffold, Myoungkun Jeoung, Lina Abdelmoti, Eun Ryoung Jang, Craig W. Vander Kooi, Emilia Galperin

Molecular and Cellular Biochemistry Faculty Publications

Shoc2 is a positive regulator of signaling to extracellular signal-regulated protein kinases 1 and 2 (ERK1/2). Shoc2 is also proposed to interact with RAS and Raf-1 in order to accelerate ERK1/2 activity. To understand the mechanisms by which Shoc2 regulates ERK1/2 activation by the epidermal growth factor receptor (EGFR), we dissected the role of Shoc2 structural domains in binding to its signaling partners and its role in regulating ERK1/2 activity. Shoc2 is comprised of two main domains: the 21 leucine rich repeats (LRRs) core and the N-terminal non-LRR domain. We demonstrated that the N-terminal domain mediates Shoc2 binding to both …

Comparative Feedstock Analysis In Setaria Viridis L. As A Model For C4 Bioenergy Grasses And Panicoid Crop Species, Carloalberto Petti, Andrew Shearer, Mizuki Tateno, Mathew J. Ruwaya, Sue E. Nokes, Tom Brutnell, Seth Debolt

Horticulture Faculty Publications

Second generation feedstocks for bioethanol will likely include a sizable proportion of perennial C4 grasses, principally in the Panicoideae clade. The Panicoideae contain agronomically important annual grasses including Zea mays L. (maize), Sorghum bicolor (L.) Moench (sorghum), and Saccharum officinarum L. (sugar cane) as well as promising second generation perennial feedstocks including Miscanthus × giganteus and Panicum virgatum L. (switchgrass). The underlying complexity of these polyploid grass genomes is a major limitation for their direct manipulation and thus driving a need for rapidly cycling comparative model. Setaria viridis (green millet) is a rapid cycling C4 panicoid grass with a relatively …

Polypeptides, Systems, And Methods Useful For Detecting Glucose, Sylvia Daunert, Kendrick Turner, Smita Joel, Laura Rowe

Chemistry Faculty Patents

The presently-disclosed subject matter provides biosensors for detecting molecules of interest. The biosensors include a polypeptide capable of selectively-binding glucose, wherein the polypeptide molecule is selected from: an unnatural analogue of wild type glucose binding protein; a fragment of wild type glucose binding protein; and an unnatural analogue fragment of wild type glucose binding protein.

Biomarkers For Ovarian Cancer, Eric T. Fung, Frederick Ueland, J. R. Van Nagell, Paul D. Depriest, Andre T. Baron

Epidemiology and Environmental Health Faculty Patents

The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying ovarian cancer status in a patient. In particular, the biomarkers of this invention are useful to classify a subject sample as ovarian cancer, ovarian cancer of low malignant potential, benign ovarian disease or other malignant condition. The biomarkers can be detected by SELDI mass spectrometry.

Reversion Of The Arabidopsis Rpn12a-1 Exon-Trap Mutation By An Intragenic Suppressor That Weakens The Chimeric 5' Splice Site, Jasmina Kurepa, Yan Li, Jan A. Smalle

Plant and Soil Sciences Faculty Publications

BACKGROUND: In the Arabidopsis 26S proteasome mutant rpn12a-1, an exon-trap T-DNA is inserted 531 base pairs downstream of the RPN12a STOP codon. We have previously shown that this insertion activates a STOP codon-associated latent 5' splice site that competes with the polyadenylation signal during processing of the pre-mRNA. As a result of this dual input from splicing and polyadenylation in the rpn12a-1 mutant, two RPN12a transcripts are produced and they encode the wild-type RPN12a and a chimeric RPN12a-NPTII protein. Both proteins form complexes with other proteasome subunits leading to the formation of wild-type and mutant proteasome versions. The net result …

Qualitative Data Archiving In The Digital Age: Strategies For Data Preservation And Sharing, Lisa Cliggett

Anthropology Faculty Publications

Given the combination of recent mandates from funding agencies for data management plans and data sharing, and the explosion of data in digital form over the past two decades, it is time for the qualitative social science community to embrace digital archiving as an inherent component of research methodology. Archiving digital data ensures, at the least, that an individual scholar’s data is preserved and accessible to the user many decades into his or her career. Digital archiving also has the potential to preserve for the broader scholarly community, the full range of social science knowledge far beyond an individual researcher’s …

Protection Of Dietary Polyphenols Against Oral Cancer, Yijian Ding, Hua Yao, Yanan Yao, Leonard Yenwong Fai, Zhuo Zhang

Toxicology and Cancer Biology Faculty Publications

Oral cancer represents a health burden worldwide with approximate 275,000 new cases diagnosed annually. Its poor prognosis is due to local tumor invasion and frequent lymph node metastasis. Better understanding and development of novel treatments and chemo-preventive approaches for the preventive and therapeutic intervention of this type of cancer are necessary. Recent development of dietary polyphenols as cancer preventives and therapeutic agents is of great interest due to their antioxidant and anti-carcinogenic activities. Polyphenols may inhibit carcinogenesis in the stage of initiation, promotion, or progression. In particular, dietary polyphenols decrease incidence of carcinomas and exert protection against oral cancer by …

Nfat5 Represses Canonical Wnt Signaling Via Inhibition Of Β-Catenin Acetylation And Participates In Regulating Intestinal Cell Differentiation, Qingding Wang, Yuning Zhou, Piotr G. Rychahou, Chunming Liu, Heidi L. Weiss, B. Mark Evers

Surgery Faculty Publications

The intestinal mucosa undergoes a continual process of proliferation, differentiation, and apoptosis, which is regulated by multiple signaling pathways. The Wnt/β-catenin pathway has a critical role in this process. Previously, we have shown that the calcineurin-dependent nuclear factor of activated T cell (NFAT) is involved in the regulation of intestinal cell differentiation, as noted by the alteration of brush-border enzyme intestinal alkaline phosphatase (IAP) activity. Here, we show that calcineurin-independent NFAT5 interacts with β-catenin to repress Wnt signaling. We found that overexpression of NFAT5 inhibits, whereas knockdown of NFAT5 increases, TOPflash reporter activity and the expression of …

Influence Of Media Composition On The Growth Rate Of Chlorella Vulgaris And Scenedesmus Acutus Utilized For Co2 Mitigation, Czarena L. Crofcheck, Xinyi E, Aubrey Shea, Michael D. Montross, Mark Crocker, Rodney Andrews

Biosystems and Agricultural Engineering Faculty Publications

Atmospheric carbon dioxide levels have increased since the industrial revolution due to increasing combustion of fossil fuels. One possible CO₂ mitigation strategy is the use of microalgae for mitigation of CO₂. This paper focuses on the influence of media composition on the growth rate of two microalgae strains, Chlorella vulgaris and Scenedesmus actus. A KNO₃ based medium was found to work better for Chlorella, while a urea based equivalent worked better for Scenedesmus. The urea based media investigated here resulted in growth similar to that found with previously reported KNO₃ based media. …

Envelope Determinants Of Equine Lentiviral Vaccine Protection, Jodi K. Craigo, Corin Ezzelarab, Sheila J. Cook, Chong Liu, David W. Horohov, Charles J. Issel, Ronald C. Montelaro

Veterinary Science Faculty Publications

Lentiviral envelope (Env) antigenic variation and associated immune evasion present major obstacles to vaccine development. The concept that Env is a critical determinant for vaccine efficacy is well accepted, however defined correlates of protection associated with Env variation have yet to be determined. We reported an attenuated equine infectious anemia virus (EIAV) vaccine study that directly examined the effect of lentiviral Env sequence variation on vaccine efficacy. The study identified a significant, inverse, linear correlation between vaccine efficacy and increasing divergence of the challenge virus Env gp90 protein compared to the vaccine virus gp90. The report demonstrated approximately 100% protection …

Assessing Insecticide Hazard To Bumble Bees Foraging On Flowering Weeds In Treated Lawns, Jonathan L. Larson, Carl T. Redmond, Daniel A. Potter

Entomology Faculty Publications

Maintaining bee-friendly habitats in cities and suburbs can help conserve the vital pollination services of declining bee populations. Despite label precautions not to apply them to blooming plants, neonicotinoids and other residual systemic insecticides may be applied for preventive control of lawn insect pests when spring-flowering weeds are present. Dietary exposure to neonicotinoids adversely affects bees, but the extent of hazard from field usage is controversial. We exposed colonies of the bumble bee Bombus impatiens to turf with blooming white clover that had been treated with clothianidin, a neonicotinoid, or with chlorantraniliprole, the first anthranilic diamide labeled for use on …

Molecular Remodeling Of Tip Links Underlies Mechanosensory Regeneration In Auditory Hair Cells, Artur A. Indzhykulian, Ruben Stepanyan, Anastasiia Nelina, Kateri J. Spinelli, Zubair M. Ahmed, Inna A. Belyantseva, Thomas B. Friedman, Peter G. Barr-Gillespie, Gregory I. Frolenkov

Physiology Faculty Publications

Sound detection by inner ear hair cells requires tip links that interconnect mechanosensory stereocilia and convey force to yet unidentified transduction channels. Current models postulate a static composition of the tip link, with protocadherin 15 (PCDH15) at the lower and cadherin 23 (CDH23) at the upper end of the link. In terminally differentiated mammalian auditory hair cells, tip links are subjected to sound-induced forces throughout an organism's life. Although hair cells can regenerate disrupted tip links and restore hearing, the molecular details of this process are unknown. We developed a novel implementation of backscatter electron scanning microscopy to visualize simultaneously …

Dynamic Functions Of Rhoa In Tumor Cell Migration And Invasion, Kathleen L. O'Connor, Min Chen

Markey Cancer Center Faculty Publications

RhoA is one of the more extensively studied members of the Rho family of small GTPase where it is most readily recognized for its contributions to actin-myosin contractility and stress fiber formation. Accordingly, RhoA function during cell migration has been relegated to the rear of the cell where it mediates retraction of the trailing edge. However, RhoA can also mediate membrane ruffling, lamellae formation and membrane blebbing, thus suggesting an active role in membrane protrusions at the leading edge. With the advent of fluorescence resonance energy transfer (FRET)-based Rho activity reporters, RhoA has been shown to be active at the …

Archaeal Nucleosome Positioning In Vivo And In Vitro Is Directed By Primary Sequence Motifs, Narasimharao Nalabothula, Liqun Xi, Sucharita Bhattacharyya, Jonathan Widom, Ji-Ping Wang, John N. Reeve, Thomas J. Santangelo, Yvonne N. Fondufe-Mittendorf

Molecular and Cellular Biochemistry Faculty Publications

Background: Histone wrapping of DNA into nucleosomes almost certainly evolved in the Archaea, and predates Eukaryotes. In Eukaryotes, nucleosome positioning plays a central role in regulating gene expression and is directed by primary sequence motifs that together form a nucleosome positioning code. The experiments reported were undertaken to determine if archaeal histone assembly conforms to the nucleosome positioning code.

Results: Eukaryotic nucleosome positioning is favored and directed by phased helical repeats of AA/TT/AT/TA and CC/GG/CG/GC dinucleotides, and disfavored by longer AT-rich oligonucleotides. Deep sequencing of genomic DNA protected from micrococcal nuclease digestion by assembly into archaeal nucleosomes has established that …

Single-Step Electrochemical Functionalization Of Double-Walled Carbon Nanotube (Dwcnt) Membranes And The Demonstration Of Ionic Rectification, Xin Zhan, Ji Wu, Zhiqiang Chen, Bruce J. Hinds

Chemistry Faculty Publications

Carbon nanotube (CNT) membranes allow the mimicking of natural ion channels for applications in drug delivery and chemical separation. Double-walled carbon nanotube membranes were simply functionalized with dye in a single step instead of the previous two-step functionalization. Non-faradic electrochemical impedance spectra indicated that the functionalized gatekeeper by single-step modification can be actuated to mimic the protein channel under bias. This functional chemistry was proven by a highly efficient ion rectification, wherein the highest experimental rectification factor of ferricyanide was up to 14.4. One-step functionalization by electrooxidation of amine provides a simple and promising functionalization chemistry for the application of …

Relationship Between Serum And Brain Carotenoids, Α-Tocopherol, And Retinol Concentrations And Cognitive Performance In The Oldest Old From The Georgia Centenarian Study, Elizabeth J. Johnson, Rohini Vishwanathan, Mary Ann Johnson, Dorothy B. Hausman, Adam Davey, Tammy M. Scott, Robert C. Green, L. Stephen Miller, Marla Gearing, John Woodard, Peter T. Nelson, Hae-Yun Chung, Wolfgang Schalch, Jonas Wittwer, Leonard W. Poon

Sanders-Brown Center on Aging Faculty Publications

Oxidative stress is involved in age-related cognitive decline. The dietary antioxidants, carotenoids, tocopherols, and vitamin A may play a role in the prevention or delay in cognitive decline. In this study, sera were obtained from 78 octogenarians and 220 centenarians from the Georgia Centenarian Study. Brain tissues were obtained from 47 centenarian decedents. Samples were analyzed for carotenoids, α-tocopherol, and retinol using HPLC. Analyte concentrations were compared with cognitive tests designed to evaluate global cognition, dementia, depression and cognitive domains (memory, processing speed, attention, and executive functioning). Serum lutein, zeaxanthin, and β-carotene concentrations were most consistently related to better cognition …

Juvenile Hormone And Insulin Regulate Trehalose Homeostasis In The Red Flour Beetle, Tribolium Castaneum, Jingjing Xu, Zhentao Sheng, Subba R. Palli

Entomology Faculty Publications

Insulin/IGF-1 signaling (IIS) has been well studied for its role in the control of life span extension and resistance to a variety of stresses. The Drosophila melanogaster insulin-like receptor (InR) mutant showed extended life span due to reduced juvenile hormone (JH) levels. However, little is known about the mechanism of cross talk between IIS and JH in regulation of life span extension and resistance to starvation. In the current study, we investigated the role of IIS and JH signaling in regulation of resistance to starvation. Reduction in JH biosynthesis, JH action, or insulin-like peptide 2 (ILP2) syntheses by RNA interference …

Currencies Of Mutualisms: Sources Of Alkaloid Genes In Vertically Transmitted Epichloae, Christopher L. Schardl, Carolyn A. Young, Juan Pan, Simona Florea, Johanna E. Takach, Daniel G. Panaccione, Mark L. Farman, Jennifer S. Webb, Jolanta Jaromczyk, Nikki D. Charlton, Padmaja Nagabhyru, Li Chen, Chong Shi, Adrian Leuchtmann

Plant Pathology Faculty Publications

The epichloae (Epichloë and Neotyphodium species), a monophyletic group of fungi in the family Clavicipitaceae, are systemic symbionts of cool-season grasses (Poaceae subfamily Poöideae). Most epichloae are vertically transmitted in seeds (endophytes), and most produce alkaloids that attack nervous systems of potential herbivores. These protective metabolites include ergot alkaloids and indole-diterpenes (tremorgens), which are active in vertebrate systems, and lolines and peramine, which are more specific against invertebrates. Several Epichloë species have been described which are sexual and capable of horizontal transmission, and most are vertically transmissible also. Asexual epichloae are mainly or exclusively vertically transmitted, and many are …

Green Tea Polyphenols And Sulfasalazine Have Parallel Anti-Inflammatory Properties In Colitis Models, Helieh S. Oz, Theresa Chen, Willem J.S. De Villiers

Internal Medicine Faculty Publications

Background: There is no cure for autoimmune chronic inflammatory bowel disease (IBD). IBD patients commonly use complementary and alternative medications of which the safety, efficacy, and interaction with standard-of-care therapies are not fully known. Thus the consequences can become life-threatening. Sulfasalazine commonly used in IBD, potentially has severe adverse effects, including infertility, pulmonary fibrosis, lack of response, and ultimately patients may require intestinal resection. We hypothesized that green tea polyphenols (GrTP, EGCG) and sulfasalazine have similar anti-inflammatory properties.

Methods: BALB/c mice received Dextran sodium sulfate (DSS) to induce colitis (ulcerative colitis model). Exposure of IL-10 deficient mice (BALB/c-background) to normal …

A New Approach To Light-Weight Ablators Analysis: From Micro-Tomography Measurements To Statistical Analysis And Modeling, Nagi N. Mansour, Francesco Panerai, Alexandre Martin, Dilworth Y. Parkinson, Alastair A. Macdowell, Abdelmoula Haboub, Timothy A. Sandstrom, Tony Fast, Gerard L. Vignoles, Jean Lachaud

Mechanical Engineering Faculty Publications

The morphology characteristics and ablation behavior of a highly porous carbon fiber preform are studied using a combined experimental/numerical approach. Morphological characterization of the three-dimensional structure of the material is performed by hard X-rays synchrotron micro-tomography at the Advanced Light Source of Lawrence Berkeley National Laboratory. The resulting micro-tomography voxels are used to compute geometrical properties of the carbon preform, like porosity, specific surface area and tortuosity, that are otherwise indirectly measured through experimental techniques. The reconstructed volumes are used to build a computational grid for numerical simulations of the fibers' ablation. By modeling the diffusion of oxygen through the …

Flow-Tube Oxidation Experiments On The Carbon Preform Of Pica, Francesco Panerai, Alexandre Martin, Nagi N. Mansour, Steven A. Sepka, Jean Lachaud

Mechanical Engineering Faculty Publications

Oxidation experiments on the carbon preform of a phenolic-impregnated carbon ablator were performed in the NASA Ames ow-tube reactor facility, at temperatures between 700 and 1300 K, under dry air gas at pressures between 103 and 104 Pa. Mass loss, volumetric recession and density changes were measured at different test conditions. An analysis of the diffusion/reaction competition within the porous material, based on the Thiele number, allowed us to identify low temperature and low pressure conditions to be dominated by in-depth volume oxidation. Experiments above 1000 K were found at transition conditions, where diffusion and reaction occur at similar scales. …

Block Copolymer Self-Assembled And Cross-Linked Nanoassemblies For Combination Delivery Of Iron Oxide And Doxorubicin, Daniel Scott, Yihwa Beabout, Robert J. Wydra, Mo Dan, Robert A. Yokel, J. Zach Hilt, Younsoo Bae

Pharmaceutical Sciences Faculty Publications

We describe the development of nanoscale polymer drug carriers for the combinational delivery of an anticancer drug (doxorubicin: DOX) along with super paramagnetic iron oxide nanoparticles (IONPs). The drug molecules were electrostatically loaded into both block copolymer self-assembled nanoassemblies (SNAs) and cross-linked nanoassemblies (CNAs). Both nanoassemblies entrapped DOX and IONPs either individually or in tandem, maintaining sub-100 nm diameter. The IONP-loaded nanoassemblies generated heat in the presence of an alternating magnetic field (AMF). Incorporation of the drug payload, DOX, showed no adverse effects on the heating profile. Drug release from the SNAs and CNAs was accelerated as temperature increased from …