Digital Commons Network^™

The Non-Steroidal Anti-Inflammatory Drugs-Myocardial Infarction Association: An Investigation Of Kentucky Medicaid Prescription Claims, Leonard A. Gordon

Theses and Dissertations--Epidemiology and Biostatistics

Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most widely used medications globally. There are generally two types: selective (COX-2) and traditional NSAIDs (COX-1). They are primarily used for the treatment of pain. They gained attention after a study about their basic mechanisms highlighted their toxicity.

Several studies have reported an association between NSAIDs and risk of myocardial infarction (MI). However, the direction of the relationship is not conclusive. Further studies are needed to ascertain the direction of this relationship and evaluate the present situation with available drugs. Due to the seriousness of cardiovascular diseases as one of the leading cause …

Incorporating Dynamic Flame Behavior Into The Scaling Laws Of Wildland Fire Spread, Brittany A. Adam

Theses and Dissertations--Mechanical and Aerospace Engineering

A challenge for fire researchers is obtaining data from those fires that are most dangerous and costly. While it is feasible to instrument test beds, test plots, and small prescribed burns for research, it is uncommon to successfully instrument an active wildland fire. With a focus on very specific facets of wildland fire, researchers have created many unique models utilizing matchsticks, cardboard, liquid fuel, excelsior, plywood, live fuels, dead fuels, and wood cribs of different packing densities. Such scale models, however, only serve as valid substitutes for the full-scale system when all functional relations of the scale model are made …

A Subsystem Identification Approach To Modeling Human Control Behavior And Studying Human Learning, Xingye Zhang

Theses and Dissertations--Mechanical and Aerospace Engineering

Humans learn to interact with many complex dynamic systems such as helicopters, bicycles, and automobiles. This dissertation develops a subsystem identification method to model the control strategies that human subjects use in experiments where they interact with dynamic systems. This work provides new results on the control strategies that humans learn.

We present a novel subsystem identification algorithm, which can identify unknown linear time-invariant feedback and feedforward subsystems interconnected with a known linear time-invariant subsystem. These subsystem identification algorithms are analyzed in the cases of noiseless and noisy data.

We present results from human-in-the-loop experiments, where human subjects in- teract …

Clinical And Pathologic Significance Of Integrin Α6Β4 Expression In Human Malignancies, Rachel L. Stewart

Theses and Dissertations--Clinical and Translational Science

Integrins are cellular adhesion molecules that bind cells to the extracellular matrix. The integrin α6β4, a receptor for laminins, is predominantly expressed on epithelial cells where it is present at the basal surface adjacent to the basement membrane. This integrin plays a critical role in maintaining normal cellular functions, yet has also been implicated in promoting invasion and metastasis in human malignancies. While overexpression of the integrin α6β4 has been detected in select human cancers, the clinical significance of integrin α6β4 expression in a number of malignancies has not been determined. The purpose of this study was to examine integrin …

The Metabolomic Effects Of Metformin On Colon Cancer, Jennifer W. Harris

Theses and Dissertations--Clinical and Translational Science

Metformin is an oral biguanide that is prescribed to over 120 million people worldwide for the treatment of conditions including type II diabetes mellitus, polycystic ovarian syndrome, and gestational diabetes. This hypoglycemic agent is rapidly emerging as a potential cost-effective anti-oncogenic agent. Over the past decade multiple epidemiologic studies have consistently associated metformin with decreased cancer incidence and cancer-related mortality. More recently numerous preclinical and clinical studies have demonstrated anti-cancer effects of metformin, leading to the proposal of numerous clinical trials to better understand this drug and its mechanism of action.

Previously experts believed metformin primarily targeted AMP-activated protein kinase …

The Role Of Apob-Containing Lipoproteins In Abdominal Aortic Aneurysm, Jing Liu

Theses and Dissertations--Pharmacology and Nutritional Sciences

Abdominal aortic aneurysm (AAA) is a devastating disease that exhibits permanent lumen expansion typically in the infrarenal aorta. AAA is prevalent among aged population, especially in males. Despite the incidence in women is lower, studies indicate the tortuosity is more severe and aortic rupture risk is higher in women. In most patients, AAA remains asymptomatic until it ruptures leading to sudden and fatal hemorrhage.

To date, there is no proven medical therapy that can prevent the expansion or rupture. Human observational studies implicate the presence of AAA is associated with both high plasma low-density lipoprotein-cholesterol (HDL-C) and low plasma high-density …

Tumor-Derived Proteins And Mitochondrial Dysfunction In Lung Cancer-Induced Cachexia, Julie B. Mclean

Theses and Dissertations--Physiology

Lung tumors secrete multiple factors that contribute to cachexia, a severe wasting syndrome that includes loss of muscle mass, weakness, and fatigue. 80% of advanced lung cancer patients experience cachexia, which cannot be reversed by nutritional interventions, diminishes response to and tolerance of cancer treatments, and increases morbidity and mortality. Despite a multitude of clinical trials, there are currently no approved treatments. This deficiency suggests that not all of the factors that contribute to cachexia have been identified.

Cancer is frequently accompanied by an increase in cyclooxygenase-2 (COX-2), a hallmark of inflammation. Clinical trials for COX-2 inhibitors have resulted in …

The Skeletal Muscle Molecular Clock Regulates The Timing Of Substrate Metabolism And The Circadian Expression Of Titin-Cap, Brian A. Hodge

Theses and Dissertations--Physiology

Skeletal muscle is a major contributor to whole-body metabolism as it serves as a depot for both glucose and amino acids, and is a highly metabolically active tissue. An intrinsic molecular clock mechanism exists within skeletal muscle that regulates the timing of physiological processes. A key function of the clock is to regulate the timing of metabolic processes to anticipate time of day changes in environmental conditions. The purpose of this study was to identify metabolic genes that are expressed in a circadian manner and determine if these genes are regulated downstream of the intrinsic molecular clock by assaying gene …

Global-Scale Analysis Of The Dynamic Transcriptional Adaptations Within Skeletal Muscle During Hypertrophic Growth, Tyler Kirby

Theses and Dissertations--Physiology

Skeletal muscle possesses remarkable plasticity in responses to altered mechanical load. An established murine model used to increase mechanical load on a muscle is the surgical removal of the gastrocnemius and soleus muscles, thereby placing a functional overload on the plantaris muscle. As a consequence, there is hypertrophic growth of the plantaris muscle. We used this model to study the molecular mechanisms regulating skeletal muscle hypertrophy.

Aged skeletal muscle demonstrates blunted hypertrophic growth in response to functional overload. We hypothesized that an alteration in gene expression would contribute to the blunted hypertrophic response observed with aging. However, the difference in …

Psychological Well-Being Among Breast Cancer Survivors: Factors That Influence Transition From Primary Treatment To Early Survivorship, Celestine G. Gochett

Theses and Dissertations--Nursing

Eudaimonic psychological well-being (PWB) refers to a lifelong process of purposeful engagement in goal-driven tasks or activities resulting in positive psychological functioning. These activities reflect autonomy, purpose in life, self-acceptance, personal growth, positive relations with others, and environmental mastery. While more women are surviving breast cancer, they face a multitude of late and long term physiopsychosocial challenges that result from being diagnosed and treated. Protective health effects associated with the experience of PWB can potentially mitigate ill-being and benefit overall health of breast cancer survivors (BCS). Adequate preparation by healthcare teams to transition them from primary treatment to early survivorship …

A Comparative Life Cycle Assessment Of Denim Jeans And A Cotton T-Shirt: The Production Of Fast Fashion Essential Items From Cradle To Gate, Tara Hackett

Theses and Dissertations--Retailing and Tourism Management

As a result of harmful textile production, sustainability has become the movement by which the apparel industry explores solutions to improve procedures in fashion design to maintain a healthy environment. However, the issue is consumers trust the sustainability claims and marketing materials of apparel products at face value without knowing its environmental impact. The overall purpose of this research was to compare the environmental implications of widely produced and owned apparel products through a life cycle assessment approach. This life cycle assessment study examines key environmental impact categories of the materials and production phase (cradle to gate) of a pair …

Efficacy Of Dormant Season Herbicide Application On Control Of Japanese Honeysuckle (Lonicera Japonica) For Habitat Restoration In Kentucky, Jason L. Weese

Theses and Dissertations--Forestry and Natural Resources

Kentucky’s disappearing native grassland communities provide habitat for native flora and fauna. A study was conducted to compare the efficacy of herbicides in control of the invasive Japanese honeysuckle (Lonicera japonica) applied at times when most native species are dormant. Six herbicide mixtures (glyphosate, glyphosate + imazapyr, glyphosate + imazapic, imazapyr, triclopyr + difluphenzopyr, and metsulfuron + difluphenzopyr) were applied in three seasons to assess the effect of application timing of each mixture on honeysuckle control. Herbicides were applied with a CO² pressurized sprayer at three sites in a randomized complete block design. Pretreatment sampling indicated that …

A Long-Term Investigation Of The Federally Threatened Desert Tortoise (Gopherus Agassizii) At A Wind Energy Facility In Southern California, Mirza Mickey Agha

Theses and Dissertations--Forestry and Natural Resources

With the recent increase in utility-scale wind energy development and current climate variation in the desert southwest US, researchers have become increasingly concerned with the reaction of wildlife and critical habitat. Understanding the relationships among monitoring efforts, climate, industrial landscapes and wildlife is critical to effective management. Given the need for information available on how these potential stressors affect terrestrial wildlife, my objective was to determine how climate variation, wind energy facilities (WEF) and monitoring efforts by researchers influence behavior and survivorship in a population of the federally threatened desert tortoise (Gopherus agassizii). Data were collected via surveys, …

An Investigation Into The Occurrence Of Batrachochytrium Dendrobatidis Infection In Plethodontid Salamander Communities Of Robinson Forest, Sarah H. Spaulding

Theses and Dissertations--Forestry and Natural Resources

Environmental and anthropogenic stressors negatively affect amphibians in a variety of ways, often increasing their vulnerability to pathogen infection and mortality. Sampling for the pathogenic fungus Batrachochytrium dendrobatidis (Bd) was conducted in order to: 1) determine the presence of chytrid infection in stream-associated plethodontid salamanders of southeastern Kentucky, and 2) evaluate differences in infection intensity between salamanders residing in intact forest streams, timber-harvested streams, and surface-mined streams. During 14 sampling sessions occurring between March, April and May of 2013, DNA samples from 306 individual salamanders within 8 species from the family Plethodontidae were collected; additional amphibians (i.e. frogs, newts) were …

Glucocorticoid-Induced Chondrocyte Cytotoxicity At Doses Recommended For Intra-Articular Therapy In Horses, Wenying Zhu

Theses and Dissertations--Veterinary Science

Intra-articular glucocorticoid injections are commonly used to treat synovitis and osteoarthritis in horses. These agents are highly effective at relieving pain, swelling, and other symptoms of joint inflammation. The drugs also have therapeutic benefits by down regulating the expression of cytokines and protease enzymes that participate in the degradation of articular cartilage. However, detrimental effects on chondrocyte function and cell viability that is independent of osteoarthritis pathogenesis have been described and linked to glucocorticoid use. These side effects are both drug- and dose-dependent. This study tested the hypothesis that manufacture recommended dosage levels of methylprednisolone, betamethasone, and triamcinolone that are …

Invasiveness Of An Exotic Gall Wasp Is Influenced By Interactions With Exotic And Endemic Organisms, Ignazio Graziosi

Theses and Dissertations--Entomology

American chestnut was once a crucial component of North American forests, but it was functionally eliminated by the introduction of the chestnut blight fungus. Chestnut is recently experiencing resurgence, but the introduced Asian chestnut gall wasp, a specialist herbivore, threatens chestnut recovery. I characterized this invasion and the interactions developing with host associates as the gall wasp spread in North America. Gall wasp dispersal is attributable to host plant distribution, effects of prevailing winds during adult insect emergence, and to topography. This knowledge may be useful to more accurately predict locations of future gall wasp infestations. Gall wasp invasiveness is …

Mechanical Exclusion And Biological Control Strategies For The Invasive Brown Marmorated Stink Bug, Halyomorpha Halys (Hemiptera: Pentatomidae), Rachelyn Dobson

Theses and Dissertations--Entomology

The brown marmorated stink bug (Halyomorpha halys) is an invasive, agriculturally destructive pest present in most of the United States. Because organic farmers have few effective control options to combat this pest, three potential control options were investigated. Screens of different mesh sizes (0.42cm, 0.32cm, and 0.10cm) that covered vegetable crops were evaluated for their ability to exclude H. halys, allow the entry of beneficial insects, and protect the crop from various types of damage. H. halys and native stink bug egg masses were placed in an organic field and evidence of predation and parasitism from native …

Taxonomic And Molecular Studies In Cleridae And Hemiptera, John Moeller Leavengood Jr.

Theses and Dissertations--Entomology

Taxonomic changes are made based on checkered beetle (Coleoptera: Cleridae) types of the Natural History Museum, London (BMNH).Lectotypes are designated (and holotypes and paralectotypes recognized) for 44 species of Hydnocerinae, including the type species for Isolemidia, Parmius, Paupris, Allelidea, Blaesiopthalmus and Lemidia, four species of Enoclerus (Clerinae), and 14 species of Cymatodera (Tillinae). Annotations include comments on additional type material, new type locality, previous (type series) locality, and questionable or missing types. Phyllobaenus pallipes(Gorham) and P. rufithorax (Gorham) are synonymized with P. flavifemoratus(Gorham), P. chapini (Wolcott) is synonymized under P. lateralis (Gorham), and P. villosus (Schenkling) …

Sex, Dementia, And Long-Term Care: Public Perspectives, Erin L. Yelland

Theses and Dissertations--Family Sciences

The current mixed methods study utilized an ecological framework to examine public perspectives toward sexual behaviors among long-term care residents with dementia. Analyzing attitudes of the public is an integral component of understanding the entire ecological system that affects the development and overall well-being of a long-term care resident. Attitudes were examined using a multiple segment factorial vignette with a probability sample of 329 respondents from a southern state. Results indicate that attitudes were not statistically affected by sex or the elapsed time since diagnosis, and age, spousal disposition, and degree of intimacy predicted attitudes. A respondent’s education level also …

Developing A Sustainable Approach To Emerald Ash Borer Management, William C. Davidson

Theses and Dissertations--Entomology

Emerald ash borer, Agrilus planipennis Fairmaire, is an invasive wood boring beetle native to eastern Asia which was first detected in North America in 2002. All North American Fraxinus (ash) species are suitable hosts and susceptible to attack. Emerald ash borer larvae feed on phloem beneath the bark of infested trees resulting in girdling and mortality in as little as five years following initial infestation. Since its introduction near Detroit, Michigan, emerald ash borer has rapidly spread throughout much of the United States and portions of Canada, threatening the persistence of ash in invaded regions.

I tested a management strategy …

Adhd Children And Mental Health Service Use: Maternal Determinants, Katarina Krizova

Theses and Dissertations--Family Sciences

The current study investigated maternal determinants of mental health service use, namely, individual child therapy, among preadolescent children diagnosed with ADHD. The Behavioral Model of Health Care Utilization (Andersen, 2008) was used as a theoretical framework for the study. Data from the last three rounds of ECLS-K dataset were employed to test a longitudinal model using Bayesian analysis. Socio-demographic variables and maternal mental health were tested as exogenous variables and mother-child relationship variables, discipline variables, and perceived maternal concern about child’s overall behavior and child’s emotional symptoms were tested as intervening variables. Results showed that only maternal mental health remained …

A Comparative Study Of Eastern And Western North American Populations Of Hippodamia Convergens (Coleoptera: Coccinellidae), Joshua S. Mccord

Theses and Dissertations--Entomology

Hippodamia convergens is a widely distributed insect predator in the United States and parts of Canada and Mexico. Several insectaries collect overwintering adults from aggregation sites in the Rocky Mountains during their winter dormancy. Collected beetles are then sold throughout the United States for augmentative biological control. This practice could have negative impacts on local populations of Hippodamia convergens in the Eastern United States. Intra-specific variation among H. convergens populations was examined for two characteristics of adults: photoperiodic induction of diapause and the presence of three known male-killing endosymbiont bacteria; Wolbachia, Spiroplasma, and Rickettsia. Four populations of H. …

New Insights Into The Function And Development Of The Soldier Caste In Termites, Li Tian

Theses and Dissertations--Entomology

The evolution of nonreproductive castes is a defining characteristic of eusociality. The function and developmental regulation of the altruistic worker and soldier caste is the central element contributing to major advantages of eusociality over solitary animals. The soldier caste is the first evolved sterile caste in termites. Their primary function is believed to be colony defense. However, the function and development of termite soldiers remains largely unknown. Because of their apparent morphological adaptation for fighting and their limited behavior repertoire, our understanding of colony defense by termite soldiers is limited to their physical defense. In addition, we know little about …

Impact Of The Healthy Hunger-Free Kids Act On Changes In The Phytochemical Content Of School Lunch Menus And Implications Of Science-Based Nutrition Education On Promoting Student Identification Of Foods High In Phytochemicals, Siddhi Lalit Shroff

Theses and Dissertations--Nutrition and Food Systems

Concern that youth do not have enough fruit and vegetable intake lead to two strategies implemented to influence intake in the school environment: the Healthy Hunger-Free Kids Act (HHFKA) and Fighting with Food: Battling Chemical Toxicity with Good Nutrition program (FF), which could influence phytochemical content and knowledge regarding phytochemicals, respectively. Individual food logs (n=468) were assessed upon completion of FF curriculum to determine whether students were able to correctly apply their nutrition knowledge regarding FF. Menus from one district were analyzed pre-HHFKA and post-HHFKA in elementary (n=156), middle (n=171), and high schools (n=171), for change in the frequency of …

The Synergistic Impact Of Income And Rurality On Timely Mammograms In A National Sample Of Women In The United States, Asri Mumpuni

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Introduction. Breast cancer is the cancer of highest incidence among women in the U.S., making it a significant public health problem. Currently, the U.S. Preventive Services recommends biennial screening mammography for women ages 50-74. This study examined the associations between socioeconomic status and rurality on breast cancer screening behavior. Methods. Data for this study come from the Centers for Disease Control and Prevention (CDC) Behavioral Risk Factor Surveillance System Survey (BRFSS). Breast cancer screening behavior was dichotomized into those who did not meet screening mammogram recommendations (2 years or more) and those who met screening mammogram recommendations (within the …

Novel Computational Methods For Transcript Reconstruction And Quantification Using Rna-Seq Data, Yan Huang

Theses and Dissertations--Computer Science

The advent of RNA-seq technologies provides an unprecedented opportunity to precisely profile the mRNA transcriptome of a specific cell population. It helps reveal the characteristics of the cell under the particular condition such as a disease. It is now possible to discover mRNA transcripts not cataloged in existing database, in addition to assessing the identities and quantities of the known transcripts in a given sample or cell. However, the sequence reads obtained from an RNA-seq experiment is only a short fragment of the original transcript. How to recapitulate the mRNA transcriptome from short RNA-seq reads remains a challenging problem. We …

Inducible Depletion Of Satellite Cells In Adult, Sedentary Mice Impairs Muscle Regenerative Capacity Without Affecting Sarcopenia, Christopher S. Fry, Jonah D. Lee, Jyothi Mula, Tyler J. Kirby, Janna R. Jackson, Fujun Liu, Lin Yang, Christopher L. Mendias, Esther E. Dupont-Versteegden, John J. Mccarthy, Charlotte A. Peterson

Physical Therapy Faculty Publications

A key determinant of geriatric frailty is sarcopenia, the age-associated loss of skeletal muscle mass and strength. Although the etiology of sarcopenia is unknown, the correlation during aging between the loss of activity of satellite cells, which are endogenous muscle stem cells, and impaired muscle regenerative capacity has led to the hypothesis that the loss of satellite cell activity is also a cause of sarcopenia. We tested this hypothesis in male sedentary mice by experimentally depleting satellite cells in young adult animals to a degree sufficient to impair regeneration throughout the rest of their lives. A detailed analysis of multiple …

Novel Citronellyl-Based Photoprobes Designed To Identify Er Proteins Interacting With Dolichyl Phosphate In Yeast And Mammalian Cells., Jeffrey S. Rush, Thangaiah Subramanian, Karunai Leela Subramanian, Fredrick O Onono, Charles J Waechter, H Peter Spielmann

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: Dolichyl phosphate-linked mono- and oligosaccharides (DLO) are essential intermediates in protein N-glycosylation, C- and O-mannosylation and GPI anchor biosynthesis. While many membrane proteins in the endoplasmic reticulum (ER) involved in the assembly of DLOs are known, essential proteins believed to be required for the transbilayer movement (flip-flopping) and proteins potentially involved in the regulation of DLO synthesis remain to be identified.

METHODS: The synthesis of a series of Dol-P derivatives composed of citronellyl-based photoprobes with benzophenone groups equipped with alkyne moieties for Huisgen "click" chemistry is now described to utilize as tools for identifying ER proteins involved in regulating …

Optimal Uses Of Biomass Resources In Distributed Applications, Joshua J. Jackson

Theses and Dissertations--Biosystems and Agricultural Engineering

Biomass production is spatially distributed resulting in high transportation costs when moving dedicated biomass crops and crop residues. A multifaceted approach was taken to address this issue as the low bulk and energy density of biomass limits transportation efficiency. Two systems were analyzed for the conversion of biomass into a denser feedstock applicable to on-farm use. Pelletization was able to densify the material into a solid fuel. Using a pilot scale flat ring pellet mill, the density of the material was able to be increased to at least 4.4 times that of uncompressed material. Pellet durability was found to be …

Characterization Of Two Biochars Derived From Horse Muck And Their Ability To Reduce Pathogen Transport In Soil, David Griffith

Theses and Dissertations--Biosystems and Agricultural Engineering

Biochars have been created and characterized from a variety livestock manure biomass including poultry, dairy, and swine. However, no research has been conducted on the physical and chemical makeup of biochar pyrolyzed from horse muck. Two horse muck derived biochars containing either straw (HS) or woodchip (HW) bedding were pyrolyzed at 700°C and characterized for their physical and chemical properties. Tests revealed both biochars had high alkalinity, moderate specific conductivity, and low surface area as compared to other biochars in the literature. HS contained more mineral structures than HW. Scanning electron microscopy presented differences in particle shape, size, and presence …