Digital Commons Network^™

Evolution Of The Vertebrate Claudin Gene Family: Insights From A Basal Vertebrate, The Sea Lamprey, Christian Mukendi, Nicholas Dean, Rushil Lala, Jeramiah J. Smith, Marianne E Bronner, Natalya V. Nikitina

Biology Faculty Publications

Claudins are major constituents of tight junctions, contributing both to their intercellular sealing and selective permeability properties. While claudins and claudin-like molecules are present in some invertebrates, the association of claudins with tight junctions has been conclusively documented only in vertebrates. Here we report the sequencing, phylogenetic analysis and comprehensive spatiotemporal expression analysis of the entire claudin gene family in the basal extant vertebrate, the sea lamprey. Our results demonstrate that clear orthologues to about half of all mammalian claudins are present in the lamprey, suggesting that at least one round of whole genome duplication contributed to the diversification of …

Social Media And Credibility Indicator: The Effects Of Bandwagon And Identity Cues Within Online Health And Risk Contexts, Xialing Lin

Theses and Dissertations--Communication

Three studies were conducted to investigate how social media affordances influence individuals’ source credibility perceptions in risk situations. The MAIN model (Sundar, 2008), warranting theory (Walther & Parks, 2002), and signaling theory (Donath, 1999) served as the theoretical framework to examine the effects of bandwagon cues and identity cues embedded in retweets and users’ profile pages for health and risk online information processing. Study One examines whether bandwagon heuristics triggered by retweets would influence individuals’ source credibility judgments. Study Two investigates how bandwagon heuristics interact with different identity heuristics in credibility heuristics on an individual level. Study Three explores bandwagon …

The Idea Model: Designing Earthquake Early Warning Messages Using Instructional Risk Communication, Nigel D. Haarstad

Theses and Dissertations--Communication

The goal of risk communication is to inform people about the risks they face and to encourage them to take appropriate action in response to that threat. To achieve this goal, risk communication scholars continuously examine the messages surrounding crises and disasters, and engage in message-testing to evaluate theory-driven message designs. Recent communication scholarship recommends that messages should including instructing information (Coombs, 2012), and should take into consideration established pedagogy based on instructional communication research (Sellnow & Sellnow, 2010). This dissertation continues to build on research which applies instructional communication scholarship to risk communication messaging. Using message-testing, this dissertation examined …

Chaos Theory And Emergent Behavior: How Ephemeral Organizations Function As Strange Attractors Through Information Communication Technologies, Morgan C. Getchell

Theses and Dissertations--Communication

Chaos theory holds that systems act in unpredictable nonlinear ways and that their behavior can only be observed, never predicted. This is an informative model for an organization in crisis. The West Virginia water contamination crisis, which began on January 9, 2014, fits the criteria of a system in chaos. Given the lack of appropriate response from the established organizations involved, many emergent organizations formed to help fill unmet informational and physical needs of the affected population. Crisis researchers have observed these ephemeral organizations for decades, but the recent proliferation of information communication technologies (ICT’s) have made their activities more …

Do Black Lives Matter In American Mainstream News Media? Two Case Studies Of Police-Involved Shootings Of Black Men Explaining A Racist Media Environment, Alfred J. Cotton Iii

Theses and Dissertations--Communication

This dissertation examines two cases of fatal police-involved shootings of Black men in order to expose the power structures perpetuated through racist media narratives assuming the officers’ behavior was justified and the unarmed men the officers killed somehow were complicit in their death. In reporting on police-involved shootings, mainstream media practices that privilege elites and officials as primary sources of information may produce a dominant media narrative that masks the marginalization and mistreatment of minorities at the hands of these officials and their institutions. The two cases under consideration here examine the “floating signifier” of race in media coverage of …

Communicating Social Support: Understanding Complexities Of Breastfeeding Communication Among African American Mothers, Nicole Rachael Peritore

Theses and Dissertations--Communication

Breast milk is the best choice for meeting the nutritional needs for an infant whenever possible. Despite the knowledge that this nutritional choice is the best choice for an infant, data demonstrates that there is a sharp decline in the rates of breastfeeding mothers. Among African Americans, breastfeeding rates are significantly lower than the national averages. Despite many of the applications of social support in communication research, there is a gap in knowledge on the social support systems in the context of breastfeeding, especially for African Americans. With the social ecological model as a framework, social support theory provides understanding …

The Link Between Smoking, Lung Cancer And Socioeconomic Factors In Kentucky, Shreya Berlia

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Background and Purpose

Lung cancer is the leading cause of cancer death and the second most diagnosed cancer in both men and women in the United States. An estimated 158,080 deaths from lung cancer are expected to occur in 2016, which will account for approximately 1 in 4 of all cancer deaths. The 1- and 5-year relative survival rates for lung cancer are 44% and 17%, respectively. The incidence rate for lung cancer has been declining since the mid-1980s in men, but only since the mid-2000s in women. Tobacco smoke is the leading cause of preventable disease and death in …

Investigations In Cryptic Species: Considerations And Applications For Estimating Detection, Occupancy, And Abundance Of Semi-Aquatic Snakes, Christian Robert Oldham

Theses and Dissertations--Forestry and Natural Resources

Snake species are notoriously difficult to study in the field due to their cryptic natural-histories and secretive behaviors. Difficulties associated with detection present challenges estimating parameters including occupancy and abundance, as well as responses to habitat degradation. Our objectives were to use Passive Integrated Transponder (PIT) telemetry to enhance detection of Queensnakes (Regina septemvittata) as compared to traditional capture-mark-recapture (CMR) survey techniques and to examine occupancy and abundance of Queensnakes and Northern Watersnakes (Nerodia sipedon) in streams of differing levels of anthropogenic impact within Central Kentucky. During 2013, we captured Queensnakes and implanted them with PIT …

A Branch Point Of Streptomyces Sulfur Amino Acid Metabolism Controls The Production Of Albomycin, Aditya Kulkarni, Yu Zeng, Wei Zhou, Steven Van Lanen, Weiwen Zhang, Shawn Chen

Pharmaceutical Sciences Faculty Publications

Albomycin (ABM), also known as grisein, is a sulfur-containing metabolite produced by Streptomyces griseus ATCC 700974. Genes predicted to be involved in the biosynthesis of ABM and ABM-like molecules are found in the genomes of other actinomycetes. ABM has potent antibacterial activity, and as a result, many attempts have been made to develop ABM into a drug since the last century. Although the productivity of S. griseus can be increased with random mutagenesis methods, understanding of Streptomyces sulfur amino acid (SAA) metabolism, which supplies a precursor for ABM biosynthesis, could lead to improved and stable production. We previously characterized the …

Effects Of Dietary Amino Acid Supplementation On Measures Of Whole-Body And Muscle Protein Metabolism In Aged Horses, Christine M. Latham

Theses and Dissertations--Animal and Food Sciences

Sarcopenia is a condition that is most common in aged animals, and is characterized by the loss of skeletal muscle mass and integrity, and can lead to physical disability and poor quality of life. Since skeletal muscle protein synthesis can be limited by the availability of amino acids, supplementation of limiting amino acids to ameliorate the progression of sarcopenia has become a topic of interest in companion animal research. Although there is some data to support the idea that amino acid supplementation improves maintenance of muscle mass in aged horses, the cellular mechanisms behind that improvement have yet to be …

Relationships Between Behavioral Measures And Productivity In Finishing Beef Cattle, Jasmyn Mimiko

Theses and Dissertations--Animal and Food Sciences

The relationship between measures of temperament, growth performance, and social hierarchy in finishing beef cattle were explored in two experiments. In experiment 1, high OCS (objective chute score) steers had periods of significantly higher ADG (P < 0.01), but OCS had no relationship with dominance ranking (P > 0.47). Conversely, slow exit velocity (EV) correlated with higher ranking (P ≤ 0.06), but EV had no relationship with performance (P > 0.37) in a competitive environment. Rank showed no relationship with performance (P > .58). In experiment 2, steers with fast EV had periods of decreased growth (P ≤ .06), intake (P ≤ .06), and gain:feed (G:F; P = 0.02). There were no interactions between …

Specialty Poultry Production: Impact Of Genotype, Feed Strategies, Alternative Feedstuffs, And Dietary Enzymes On The Growth Performance And Carcass Characteristics Of Heritage Breed Chickens, Tatijana Fisher

Theses and Dissertations--Animal and Food Sciences

There is a growing market for specialty poultry production using alternative genotypes and management systems. However, producers interested in specialty poultry production face several challenges. One challenge is that little published data exists regarding the growth and production parameters for alternative genotypes like slow-growing meat strains and heritage breeds. To address this challenge, research at the University of Kentucky examined the effect of feed strategies, alternative feedstuffs, and dietary enzymes on the growth and performance of heritage breeds of chicken used for either egg- or meat-production. The first trial documented the growth and nutrient intake of pullets from three heritage …

Posttraumatic Growth Among Older Adults With Late-Life Cancer Diagnoses, Aasha I. Hoogland

Theses and Dissertations--Gerontology

Gerontological scholarship has often focused on the ways older adults deteriorate or decline with time, but it is plausible that positive change continues into and throughout old age. Late-life psychosocial growth may even be facilitated by a life-altering event such as a cancer diagnosis, a phenomenon broadly termed posttraumatic growth (PTG). PTG has been examined in a variety of general population samples, but there is a noticeable lack of research on PTG that focuses on older adults—a population for whom cancer diagnoses are particularly prevalent. Using a mixed methods design, this dissertation was designed to advance our understanding of PTG …

Salvage Brachytherapy For Biochemically Recurrent Prostate Cancer Following Primary Brachytherapy, John M. Lacy, William A. Wilson, Raevti Bole, Li Chen, Ali S. Meigooni, Randall G. Rowland, William H. St. Clair

Urology Faculty Publications

Purpose. In this study, we evaluated our experience with salvage brachytherapy after discovery of biochemical recurrence after a prior brachytherapy procedure. Methods and Materials. From 2001 through 2012 twenty-one patients treated by brachytherapy within University of Kentucky or from outside centers developed biochemical failure and had no evidence of metastases. Computed tomography (CT) scans were evaluated; patients who had an underseeded portion of their prostate were considered for reimplantation. Results. The majority of the patients in this study (61.9%) were low risk and median presalvage PSA was 3.49 (range 17.41–1.68). Mean follow-up was 61 months. At last follow-up after reseeding, …

The Role Of Capillaries In The Lesser Ailments Of Old Age And In Alzheimer's Disease And Vascular Dementia: The Potential Of Pro-Therapeutic Angiogenesis, Charles T. Ambrose

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Apart from chronic diseases (arthritis, diabetes, etc.), old age is generally characterized by three lesser ailments: muscle weakness, minor memory lapses, and cold intolerance. This trio of complaints may have a common, underlying cause, namely, the age-associated reduced microcirculation in muscles, brain, skin, and elsewhere in the body. The Angiogenesis Hypothesis proposes that old age is in part a deficiency disease due to the decline in angiogenic (AG) factors, resulting in a reduced capillary density (CD) throughout the body. Over fifty published papers document waning levels of AG factors and/or decreased CD in various organ systems of aged animals and …

Role Of Sulfiredoxin Interacting Proteins In Lung Cancer Development, Hedy Chawsheen

Theses and Dissertations--Toxicology and Cancer Biology

Sulfiredoxin (Srx) is an antioxidant enzyme that can be induced by oxidative stress. It promotes oncogenic phenotypes of cell proliferation, colony formation, migration, and metastasis in lung, skin and colon cancers. Srx reduces the overoxidation of 2-cysteine peroxiredoxins in cells, in addition to its role of removing glutathione modification from several proteins. In this study, I explored additional physiological functions of Srx in lung cancer through studying its interacting proteins. Protein disulfide isomerase (PDI) family members, thioredoxin domain containing protein 5 (TXNDC5) and protein disulfide isomerase family A member 6 (PDIA6), were detected to interact with Srx. Therefore, I proposed …

Straight Time And Scandal: Travesti Urban Politics In São Paulo, Brazil, Christine L. Woodward

Theses and Dissertations--Geography

São Paulo, Brazil is currently pursuing a project of creative urbanism. Though city rhetoric insists this project is rooted in tolerance of sexual diversity, I suggest that city policy effectively perpetuates normative conceptions of family and respectability. Using data gathered through a series of qualitative interviews with transgender and travesti individuals living in São Paulo, I argue that the straight time of São Paulo’s creative urbanism generates exclusionary temporalities and spatialities in the city that render travestis out of time and out of place. Furthermore, I argue that travestis use their capacity to enact shame through scandals to generate temporalities …

Effects Of Feeding A Yeast-Based Supplement Containing Docosahexaenoic Acid (Dha) From A Heterotrophically Grown Microalgae, Vitamin E, And Selenium On Stallion Sperm Motion Characteristics, Lauren D. Goedde

Theses and Dissertations--Veterinary Science

The use of cooled and frozen stallion semen has become quite popular. However, there are some stallions that have sperm that are quite susceptible to cold shock. Thus, there is a need for techniques that will alter sperm so that they can withstand the stresses of cooling and freezing and thus improve pregnancy rates achieved with cooled or frozen spermatozoa. Studies have shown that a diet high in omega-3 (n-3) fatty acids can improve the motility of cooled and frozen/thawed sperm. Many of the omega-3 fatty acid products for stallions have low levels of docosahexaenoic acid (DHA) and are based …

Influence Of Structural Disturbance On Stream Function And Macroinvertebrate Communities In Upper Coastal Plain Headwater Streams, Richard Andrew Biemiller

Theses and Dissertations--Entomology

Freshwater is a resource under threat due to anthropogenic actions. Stream restoration is a common method for mitigating disturbance. Inconsistent methodologies used for evaluating restorations have drawn criticism. Limited use of baseline data for guiding stream restoration activities is of particular concern. This study was developed to elucidate metrics that differentiate reference and disturbed sites in Upper Coastal Plain streams. This information could improve resource use and successes of restorations. Structural and functional variables were examined in 10 reference and 10 streams that meet the traditional definition of disturbance and would be restoration priorities. Disturbed streams were classified into two …

Subacute Effects Of Prallethrin On Behavior Of Mosquitoes (Diptera: Culicidae) And Other Human Disease Vectors, Kyndall C. Dye

Theses and Dissertations--Entomology

The synthetic pyrethroid, prallethrin, is an active ingredient in a widely marketed ultralow volume (ULV) mosquito adulticide. Volatilized prallethrin is intended to stimulate mosquito flight, increasing overall effectiveness of the adulticide. However, field tests using volatilized prallethrin did not produce significant differences in various vector trap catches, suggesting prallethrin’s behavioral effects are not viable. Laboratory tests were conducted to evaluate prallethrin’s effect on flight behavior of adult female Asian tiger mosquitoes (Aedes albopictus). Mosquitoes were divided into three groups; a control, those exposed to volatilized prallethrin, and those exposed to a simulated ULV application at label rates. After …

Effects Of Landscape, Intraguild Interactions, And A Neonicotinoid On Natural Enemy And Pest Interactions In Soybeans, Hannah J. Penn

Theses and Dissertations--Entomology

Demand for food, fuel, and fiber has been increasing, escalating the intensification of agriculture during the past fifty years. A more comprehensive understanding of the impact of landscapes on sustainable agriculture production is required to meet the continual increase in human demand. This not only includes how chemical inputs are used but also how cultivated and surrounding landscapes are managed for ecosystem services. This research explains how land cover on landscape and farm scales impact ant and spider-mediated pest suppression. I successfully developed and optimized molecular methods to test ant gut contents from both laboratory and field-caught specimens. A multi-year …

Probing The Functional Relevance Of Reactive Hippocampal Neurogenesis In A Model Of An Alcohol Use Disorder, Chelsea Rhea Geil Nickell

Theses and Dissertations--Pharmacy

Alcoholism, or alcohol use disorders (AUDs), represent a major public health concern both locally and globally. Critically, excessive alcohol consumption results in neurodegeneration in brain regions such as the hippocampus which is known for its role in learning and memory. Recovery of hippocampal volume loss has been observed after prolonged abstinence, but the mechanisms underlying this process are not well understood. Adult neurogenesis is thought to contribute to this recovery since after alcohol exposure a reactive increase in adult neurogenesis is observed. This reactive neurogenesis (the process by which brain insult results in a compensatory increase in neurogenesis) may represent …

Breastfeeding Practices Among Native Hawaiians And Pacific Islanders, Ingrid K. Adams, Chizimuzo T.C. Okoli, Akilah Dulin Keita, Ana Maria Linares, Keiko Tanaka, Joshua R. Polanin, Annie Koempel

Dietetics and Human Nutrition Faculty Publications

Background. Breastfeeding is associated with a decreased risk of obesity in the early and adult years. Native Hawaiians and Pacific Islanders (NHPI) experience high rates of obesity which is often obfuscated with aggregated data. Using disaggregated data, we examined breastfeeding practices among NHPI. Methods. Seven databases and reference lists were searched. Two independent researchers extracted relevant studies based on predetermined criteria. Nine studies met our inclusion criteria and a meta-analysis was conducted using random-effects, inverse-various weighted models. Results. Few studies disaggregated NHPI populations when examining breastfeeding practices. Most studies were cross-sectional and our search yielded no randomized or quasirandomized control …

Risk Factors Associated With Severe Injuries In Inland Aquaculture Farms, Amah Martin

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Objective: The main objective of this study was to analyze risk factors associated with severe occupational injuries among inland aquaculture farms.

Methods: Survey results were compiled in a data set that consisted of qualitative data from 51 farmers who were interviewed between 2008 and 2011 in 10 states and the Canadian province of British Columbia. Chi-square and Fisher’s exact test were used to assess the differences in the dichotomous level of severity among several farm and injury variables. Logistic regression was used to predict the outcome of a severe injury event.

Results: Injury observations that indicated the use of raceway …

Recalibration Methodology To Compensate For Changing Fluid Properties In An Individual Nozzle Direct Injection System, Joe D. Luck, Scott A. Shearer, Brian D. Luck, Michael P. Sama

Biosystems and Agricultural Engineering Faculty Publications

Limited advancement of direct injection pesticide application systems has been made in recent years, which has hindered further commercialization of this technology. One approach to solving the lag and mixing issues typically associated with injection-based systems is high-pressure individual nozzle injection. However, accurate monitoring of the chemical concentrate flow rate can pose a challenge due to the high pressure, low flow, and changing viscosities of the fluid. A methodology was developed for recalibrating high-pressure chemical concentrate injectors to compensate for fluid property variations and evaluate the performance of this technique for operating injectors in an open-loop configuration. Specific objectives were …

Vocalization Subsystem Responses To A Temporarily Induced Unilateral Vocal Fold Paralysis, Daniel J. Croake

Theses and Dissertations--Rehabilitation Sciences

Healthy voicing is thought to be dependent on a dynamic balance of three interactive subsystems: respiration, phonation, and resonance. Theoretically, multiple patterns of subsystem interactions likely underlie healthy voice production; however surprisingly little quantitative data exists defining the nature of these subsystem production patterns and interactions across individuals.

The central aim of this study was to quantify the interactions of the vocalization subsystems in a non-perturbed and perturbed condition (induced unilateral vocal fold paralysis) in 10 vocally healthy participants. Respiratory inductance plethysmography, laryngeal aerodynamics, and acoustic formant data were used to measure the proportional contributions of, and changes to, the …

The Impact Of Food Recall On Third-Party Certification Adoption, Hongyi Zhang

Theses and Dissertations--Agricultural Economics

Food safety problems have gained national attention, and food recall is one of the most important indications of this concern. Third-party certifications have become a popular way to improve the safety and quality of products for consumers. Publications related to third-party certification usually focus on the motives and benefits of a particular certification. However, to date, no existing research investigates the effects of food recalls on certification adoption.

This study uses Probit models with a binary endogenous explanatory variable to examine the relationship between food recalls and third-party certification, based on recalls occurring between January 1, 2015 and February 18, …

Monitoring Yogurt Culture Fermentation And Predicting Fermentation Endpoint With Fluorescence Spectroscopy, Timothey P. Mains, Frederick Alan Payne, Michael P. Sama

Biosystems and Agricultural Engineering Faculty Publications

Determination of the endpoint of yogurt culture fermentation is a process parameter that could benefit from automation. The feasibility of using a fluorescence sensor technology based on 280 nm excitation and 350 nm emission to predict the endpoint of yogurt culture fermentation was investigated and compared with the endpoint prediction from a near-infrared (880 nm) light backscatter sensor. Yogurt cultures with three levels of milk solids (8%, 10%, and 12%) and three temperatures (40°C, 43°C, and 46°C) were tested with three replications in a 3 x 3 factorial design (n = 27). Prediction models were developed for each optical measurement …

Wetlands And Coastal Systems: Protecting And Restoring Valuable Ecosystems, Carmen T. Agouridis, Kyle R. Douglas-Mankin, Anna C. Linhoss, Aaron R. Mittelstet

Biosystems and Agricultural Engineering Faculty Publications

Wetlands and coastal systems are unique, highly productive, and often threatened landscapes that provide a host of services to both humans and the environment. This article introduces a five-article Wetlands and Coastal Systems Special Collection that evolved from a featured session at the 2015 ASABE Annual International Meeting in New Orleans, Louisiana. The Collection provides perspectives on tools and techniques for enhancing the protection and restoration of wetlands and coastal systems with emphasis on vegetation, hydrology, water quality, and planning. Topics span the Florida Everglades (two articles) and Virginia floodplain (one article) wetland systems and include remote sensing (one article) …

Hybrid Diesel-Electric Drivetrain For Small Agricultural Field Machines, Joseph Jackson, Joseph S. Dvorak

Biosystems and Agricultural Engineering Faculty Publications

In this project, a series electric drivetrain sized for small agricultural machinery was developed and tested. Electric drives have noted benefits in simplicity, controllability, integration with other electronics such as those that provide autonomous action, and in efficiency over a wide operating range. Their biggest drawback for agricultural use is the limited capacity of electrical energy storage. A series hybrid drivetrain provides a method to overcome these capacity constraints through the use of chemical energy storage. The series hybrid drivetrain in this research was designed using well-established components. It consisted of a diesel-electric generator, a lead acid battery pack, a …