Digital Commons Network^™

The Big "Blue" Nation: Expanding Aquaculture In Kentucky, K. Cassandra Keck

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Widespread Tau Seeding Activity At Early Braak Stages, Jennifer L. Furman, Jaime Vaquer-Alicea, Charles L. White, Nigel J. Cairns, Peter T. Nelson, Marc I. Diamond

Pathology and Laboratory Medicine Faculty Publications

Transcellular propagation of tau aggregates may underlie the progression of pathology in Alzheimer's disease (AD) and other tauopathies. Braak staging (B1, B2, B3) is based on phospho-tau accumulation within connected brain regions: entorhinal cortex (B1); hippocampus/limbic system (B2); and frontal and parietal lobes (B3). We previously developed a specific and sensitive assay that uses flow cytometry to quantify tissue seeding activity based on fluorescence resonance energy transfer (FRET) in cells that stably express tau reporter proteins. In a tauopathy mouse model, we have detected seeding activity far in advance of histopathological changes. It remains unknown whether individuals with AD also …

Risk Parameters And Assessment Of Dietary Dsrna Exposure In Folsomia Candida, Jeffrey Edward Noland

Theses and Dissertations--Entomology

Assessing the risk of transgenic crop products is essential when determining the safety of a crop for deregulation and commercialization. The Organization of Economic and Cooperative Development (OECD), International Standards Organization (ISO), and governmental regulatory agencies require a battery of tests to demonstrate the safety of a GM product against several surrogate species of organisms that perform various ecosystem services. Assays are performed using toxicology methods established for pesticides. These methods have been applied to testing the safety, specificity and fate of Bacillus thuringiensis (Bt) Cry protein toxins engineered into crop plants and information exists on the effects on non-target …

Ecological, Molecular, And Morphological Data: A Synergistic Approach To Resolve Species Limits Of Lytopylus From The Area De Conservación Guanacaste, Costa Rica (Hymenoptera: Braconidae: Agathidinae), Ilgoo Kang

Theses and Dissertations--Entomology

Lytopylus is species-rich genus of Agathidinae (Hymenoptera: Braconidae: Agathidinae), but limited information of the genus has been published. Morphological, molecular, and ecological data were compared to resolve the species limits of Lytopylus reared from caterpillars collected the Area de Conservación Guanacaste (ACG) in Costa Rica. Molecular data were initially used to hypothesize species limits, and morphological and host use data were employed to make a final decision when molecular data was indecisive, e.g., when the genetic difference between species was slight. Thirty-two new species are described with image plates of each species. Phylogenetic analyses of the barcode region of the …

“To Nurture Something That Nurtures You”: Care, Creativity, Class, And The Production Of Urban Environments In Deindustrial Michigan, Megan L. Maurer

Theses and Dissertations--Anthropology

In this dissertation I investigate how gardeners and beekeepers in a small, deindustrial city in Michigan used their activities to produce their environments. Drawing on fourteen months of ethnographic fieldwork, I consider what kind of labor gardening is. For residents of Elmwood, gardening was a way to care for households, communities, and ecosystems. Furthermore, this care was performed through a type of creative, material labor that served to address forms of alienation experienced by these individuals. While all sorts of Elmwoodites gardened, they did so in ways that were specific to their experiences of race and class. These experiences, in …

Cadmium Exposure And Residence-To-Road Proximity In Eastern Kentucky, Ellen Flora

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Background and Objectives

Cadmium is a carcinogenic and toxic metal. The general population can be exposed to cadmium from multiple sources. The purpose of this study is to evaluate the association of urinary cadmium and residence distance-to-road by performing landscape analyses in eastern Kentucky, a region where smoking prevalence is high, coal production has been a primary industry, and the landscape is predominantly undeveloped. Composite dust samples were also collected and analyzed for Cadmium (Cd), Manganese (Mn), and Zinc (Zn). The primary objective of this study is to ascertain if there is an association between participants with elevated accumulated cadmium …

A Retrospective Analysis Of Open Burning Activity In Kentucky, Jeffrey Scott Blakeman

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Objectives: To 1) summarize open burning activity observed by the Kentucky Division for Air Quality for a 12-month period and to 2) Identify distinguishing factors of areas having “high” levels of observed open burning compared to areas with “low” levels of open burning.

Methods: Data was collected by performing a records review of open burning investigation documents for the 554 investigation site visits performed by KY DAQ for the time period of January 1, 2015 through December 31, 2015.

Results: The average number of site visits per county was 4.66 visits. The source material with the highest …

Risk Factors For Self Reported Health Status In The National Health Interview Survey, Kayla Rhea Talbott

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Purpose: The aim of this study was to gain a better understanding of how individuals view and analyze their health by evaluating the relationship between self-reported health (SRH) status and several variables representing health and lifestyle characteristics. Assessing the ability of self-reported health status to measure overall health was an additional objective of the study.

Methods: A secondary, cross-sectional study was conducted using information from the 2014 National Health Interview Survey for adults 18 and older. Data were stratified and compared based on a self-rated health status of either excellent or very good, good, or fair and poor, …

Implementing Medicaid Health Homes To Provide Medication Assisted Treatment To Opioid Dependent Medicaid Beneficiaries, Page M. Smith

Kentucky Law Journal

No abstract provided.

Three Essays In Food Consumption And Health Related Issues, Fuad Mohammed Alagsam

Theses and Dissertations--Agricultural Economics

This dissertation consists of three essays that make contributions to the research on food consumption and health-related issues. Essay I elaborates on how the interactions of consumers’ beliefs and actions influence Food-Away-From-Home (FAFH) consumption and tests whether consumers compensate for the high caloric intake typically associated with FAFH by changing their behaviors during other meals. Essay II studies consumers’ choices related to time allocations for food consumption and tests how consumers’ lifestyles moderate the effect of secondary eating (eating while doing other activities) on obesity. Lastly, Essay III examines intertemporal choices, through which individuals make trade-offs between immediate gratifications and …

Further Evidence Of Early Development Of Attention To Dynamic Facial Emotions: Reply To Grossmann And Jessen, Alison Heck, Alyson J. Hock, Hannah B. White, Rachel L. Jubran, Ramesh S. Bhatt

Psychology Faculty Publications

Adults exhibit enhanced attention to negative emotions like fear, which is thought to be an adaptive reaction to emotional information. Previous research, mostly conducted with static faces, suggests that infants exhibit an attentional bias toward fearful faces only at around 7 months of age. In a recent study (Journal of Experimental Child Psychology, 2016, Vol. 147, pp. 100–110), we found that 5-month-olds also exhibit heightened attention to fear when tested with dynamic face videos. This indication of an earlier development of an attention bias to fear raises questions about developmental mechanisms that have been proposed to underlie this …

Iot Development For Healthy Independent Living, Shalom Greene

Theses and Dissertations--Electrical and Computer Engineering

The rise of internet connected devices has enabled the home with a vast amount of enhancements to make life more convenient. These internet connected devices can be used to form a community of devices known as the internet of things (IoT). There is great value in IoT devices to promote healthy independent living for older adults.

Fall-related injuries has been one of the leading causes of death in older adults. For example, every year more than a third of people over 65 in the U.S. experience a fall, of which up to 30 percent result in moderate to severe injury. …

Stored Grain Volume Measurement Using A Low Density Point Cloud, Aaron P. Turner, Joshua J. Jackson, Nicole K. Koeninger, Samuel G. Mcneill, Michael D. Montross, Mark E. Casada, Josephine M. Boac, Rumela Bhadra, Ronaldo G. Maghirang, Sidney A. Thompson

Biosystems and Agricultural Engineering Faculty Publications

This technical note presents the development of a new apparatus and data processing method to accurately estimate the volume of stored grain in a bin. Specifically, it was developed to account for the variability in surface topography that can occur in large diameter bins when partially unloaded. This was accomplished using a laser distance meter to create a low density point cloud, from which a surface was interpolated using ArcMap geoprocessing tools. The manually controlled and portable system was designed to hold the laser distance meter and provided a common reference point. The data from the laser distance meter was …

Fourth-Generation Fan Assessment Numeration System (Fans) Design And Performance Specifications, Michael P. Sama, George B. Day, Laura M. Pepple, Richard S. Gates

Biosystems and Agricultural Engineering Faculty Publications

The Fan Assessment Numeration System (FANS) is a measurement device for generating ventilation fan performance curves. Three different-sized FANS currently exist for assessing ventilation fans commonly used in poultry and livestock housing systems. All FANS consist of an array of anemometers inside an aluminum shroud that traverse the inlet or outlet of a ventilation fan. The FANS design has been updated several times since its inception and is currently in its fourth-generation (G4). The current design iteration (FANS-G4) is reported in this article with an emphasis on the hardware and software control, data acquisition systems, and operational reliability. Six FANS-G4 …

A Method For Reflectance Index Wavelength Selection From Moisture-Controlled Soil And Crop Residue Samples, Ali Hamidisepehr, Michael P. Sama, Aaron P. Turner, Ole O. Wendroth

Biosystems and Agricultural Engineering Faculty Publications

Reflectance indices are a method for reducing the dimensionality of spectral measurements used to quantify material properties. Choosing the optimal wavelengths for developing an index based on a given material and property of interest is made difficult by the large number of wavelengths typically available to choose from and the lack of homogeneity when remotely sensing agricultural materials. This study aimed to determine the feasibility of using a low-cost method for sensing the moisture content of background materials in traditional crop remote sensing. Moisture-controlled soil and wheat stalk residue samples were measured at varying heights using a reflectance probe connected …

Evaluating A New Shade For Feedlot Cattle Performance And Heat Stress, Morgan D. Hayes, Tami M. Brown-Brandl, Roger A. Eigenberg, Larry A. Kuehn, R. Mark Thallman

Biosystems and Agricultural Engineering Faculty Publications

Heat stress in cattle results in decreased feed intake, lower daily gain, and potentially death in susceptible animals under intense conditions. A study was carried out during the summer of 2013 at the USDA-ARS U.S. Meat Animal Research Center feedlot evaluating the impact of shade on environmental conditions and cattle performance. A novel two-tiered shade was used in half of the 14 pens, each holding 30 animals. The shades were designed to reduce solar heat load by 40% to 60% and to provide traveling shade across the pen, providing varied amounts of shade area as well as varied solar reduction …

Equilibrium Moisture Content Of Kabuli, Chickpea, Black Sesame, And White Sesame Seeds, Paul R. Armstrong, Elizabeth B. Maghirang, Bhadriraju Subramanyam, Samuel G. Mcneill

Biosystems and Agricultural Engineering Faculty Publications

Sesame and chickpea are important crops in Ethiopia because both are major export crops that generate much revenue for both small farmers and the country as a whole. However, there is a lack of information about the fundamental equilibrium moisture content (EMC) relationships among these crops, which would help facilitate better monitoring and storage. Therefore, EMC adsorption and desorption prediction models based on temperature (T) and relative humidity (RH) were developed for the modified Chung-Pfost and modified Henderson models for Kabuli chickpea (KC), black sesame (BS), and white sesame (WS) seeds. The samples for conducting the adsorption and desorption tests …

Toward Biochemical Conversion Of Lignocellulose On-Farm: Pretreatment And Hydrolysis Of Corn Stover In Situ, Alicia A. Modenbach, Sue E. Nokes, Michael D. Montross, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L^-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment …

Sex Differences In The Subjective Effects Of Oral Δ9-Thc In Cannabis Users, Jessica S. Fogel, Thomas H. Kelly, Philip M. Westgate, Joshua A. Lile

Behavioral Science Faculty Publications

Previous studies suggest that there are sex differences in endocannabinoid function and the response to exogenous cannabinoids, though data from clinical studies comparing acute cannabinoid effects in men and women under controlled laboratory conditions are limited. To further explore these potential differences, data from 30 cannabis users (N=18 M, 12 F) who completed previous Δ⁹-tetrahydrocannabinol (Δ⁹-THC) discrimination studies were combined for this retrospective analysis. In each study, subjects learned to discriminate between oral Δ⁹-THC and placebo and then received a range of Δ⁹-THC doses (0, 5, 15 and a “high” dose of …

Impulsivity Symptoms As Core To The Developmental Externalizing Spectrum, Michelle M. Martel, Cheri A. Levinson, Christine A. Lee, Tess E. Smith

Psychology Faculty Publications

Impulsivity is posited to be a key part of the externalizing spectrum during childhood, but this idea has received minimal empirical attention. The goal of the present investigation was to utilize network analysis to determine whether behavioral impulsivity symptoms are key components of the externalizing network across several developmental periods from preschool into adolescence. Participants were 109 preschoolers (64 % male) ages 3 to 6, 237 children (59 % male) ages 6 to 9, 372 children (59 % male) ages 10 to 13, and 357 adolescents (59 % male) ages 13 to 17 and their parents. Parents completed ratings of …

Nicotine Self-Administration Remodels Perineuronal Nets In Ventral Tegmental Area And Orbitofrontal Cortex In Adult Male Rats, Dolores B. Vazquez-Sanroman, Reyna D. Monje, Michael T. Bardo

Psychology Faculty Publications

Nicotine, a major psychoactive component of tobacco smoke, alters gamma-aminobutyric acid (GABA) modulation of dopamine neurons in the ventral tegmental area (VTA). Changes in structural neuroplasticity can occur in GABAergic parvalbumin (PRV) positive neurons, which are enveloped by structures of the extracellular matrix called perineuronal nets (PNNs). In the current study, rats were trained to self-administer intravenous nicotine (0.03 mg/kg/infusion) for 21 days in 1-hour daily sessions with an incrementing fixed ratio requirement; a control group received saline infusions. At either 45 minutes or 72 hours after the last session, immunofluorescence measurements for PNNs, PRV and c-Fos were conducted. In …

Cellular And Molecular Mechanisms Underlying Alcohol-Induced Aggressiveness Of Breast Cancer, Yongchao Wang, Mei Xu, Zun-Ji Ke, Jia Luo

Pharmacology and Nutritional Sciences Faculty Publications

Breast cancer is a leading cause of morbidity and mortality in women. Both Epidemiological and experimental studies indicate a positive correlation between alcohol consumption and the risk of breast cancer. While alcohol exposure may promote the carcinogenesis or onset of breast cancer, it may as well enhance the progression and aggressiveness of existing mammary tumors. Recent progress in this line of research suggests that alcohol exposure is associated with invasive breast cancer and promotes the growth and metastasis of mammary tumors. There are multiple potential mechanisms involved in alcohol-stimulated progression and aggressiveness of breast cancer. Alcohol may increase the mobility …

A Comparison Of Sex Differences In Psychotropic Medication Use In Older People With Alzheimer's Disease In The Us And Finland, Daniela C. Moga, Heidi Taipale, Anna-Maija Tolppanen, Antti Tanskanen, Jari Tiihonen, Sirpa Hartikainen, Qishan Wu, Gregory A. Jicha, Danijela Gnjidic

Pharmacy Practice and Science Faculty Publications

Aims—Given the high prevalence of psychotropic medication use in people with dementia and the potential for different prescribing practices in men and women, our study aimed to investigate sex differences in psychotropic medication use in older adults with Alzheimer’s disease (AD) living in the US and Finland.

Methods—We used data collected between 2005 and 2011 as part of the National Alzheimer’s Coordinating Center (NACC) in the US, and Medication use and Alzheimer’s disease (MEDALZ) cohorts in Finland. We evaluated psychotropic medication use (antidepressant, antipsychotic, anxiolytic, sedative, or hypnotic) in participants aged 65 years or older. We employed multivariable …

Metabolic Reprogramming Of Human Lung Cancer Cells And Ex Vivo Tissues Revealed By Uhr-Ftms Analysis Of Small Amino And Carboxyl Metabolites, Ye Yang

Theses and Dissertations--Toxicology and Cancer Biology

Studies were carried out to understand how human lung cancer cells and human ex vivo lung cancer tissues that are metabolically reprogramed compared with analogous non-cancer cells or non-cancer tissues. A Stable isotope resolved metabolomics (SIRM) approach was used to fulfill this aim by employing ¹³C, ²H or ¹⁵N labeled metabolic precursors like ¹³C₆-Glc, ¹³C₂-Gly, ²H₂-Gly, ²H₃-Ser, ¹³C₅, ¹⁵N₂-Gln to trace the flow of the labeled atoms into the down stream metabolic network. NMR and mass spectrometry are two …

Effects Of Chromium On Mouse Splenic T Lymphocytes And Effects Of Ethanol Exposure During Early Neurodevelopment On Behaviors In Mice, Lu Dai

Theses and Dissertations--Toxicology and Cancer Biology

The dissertation consists of three major projects with the focus on the immunotoxicity of chromium and the behavior disorders caused by early ETOH exposure respectively.

Hexavalent chromium [Cr(VI)] is widely used in various industrial processes and has been recognized as a carcinogen. As the first line of host defense system, the immune system can be a primary target of Cr(VI). T cell population represents a major arm of the immune system that plays a critical role in host anti-tumor immunity. Dysfunction of T cells compromises host anti-tumor immunity resulting in oncogenesis. Using mouse splenic T cells as an in vitro …

Nucleotide Excision Repair: Impacts Of Environmental Carcinogens And Its Role In Cancer Susceptibility In Appalachian Kentucky, Nathaniel C. Holcomb

Theses and Dissertations--Toxicology and Cancer Biology

Lung cancer is a particularly devastating disease, accounting for the most deaths among all cancer types in the United States. Despite a reduction in the country’s smoking rates, cigarette smoking remains the number one risk factor for lung cancer. Additionally arsenic exposure, which occurs primarily through contaminated drinking water in the U.S., is associated with increased lung cancer incidence. The nucleotide excision repair (NER) pathway is critical for maintenance of genomic fidelity, removing DNA lesions that could otherwise promote DNA mutations and drive carcinogenesis. Tobacco smoking introduces significant amounts of DNA damage and produces characteristic DNA mutations found in lung …

Respiratory Function And Associated Risk Factors In The Kentucky Women’S Health Registry, Negash Amsalu

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

From the University of Kentucky Women’s Health Registry Database (KWHRD) a total of 1838 women were voluntarily asked to undergo Spirometric respiratory function tests. These women came to the clinic for various reasons. Various independent variables (risk factors) were also recorded for these women. Among these risk factors were: age, BMI, smoking status, asthmatic status, educational status and ethnicity.

The purpose of the study was to investigate whether any of the risk factors had significant impacts on women’s respiratory health. Appropriate categorizations were tabulated for each risk factor so that comparisons would be possible and easier. The motivation of the …

The Effects Of Improved Water And Sanitation Access On Under Five Child Diarrhea In Peru, April Ballard

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Significance: Diseases related to poor or lack of access to clean water, sanitation, and hygiene negatively impact health, education, and development worldwide and cause millions of deaths in children each year. Health outcomes associated with lack of access to clean water and sanitation are under-evaluated.

Aim: To assess the effect of improved water and sanitation infrastructure development on child morbidity in Peru by investigating diarrhea in children under five years of age.

Methods: The child, family, and household data from the Peruvian Demographic and Health Surveys (DHS) were used to evaluate the association between access to water …

Dusty Occupations And Pulmonary Obstruction In Kentucky: A Proportionate Mortality Analysis, Neil Bradford Horsley

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Background

Chronic obstructive pulmonary disease (COPD) is the third leading cause of death in the United States. Occupational exposure to vapors, gases, dusts, or fumes (VGDFs), in combination with smoking, significantly increases the risk of developing COPD. Amongst agricultural workers, exposure to pesticides has been found to cause significantly elevated rates of annual decline in lung function, ultimately leading to a clinical diagnosis of pulmonary obstruction if the period of exposure is sufficient.

Purpose

It is hypothesized that, when compared to the standard population, workers in “dusty” occupations (where VGDF workplace exposure is significant) die at significantly elevated rates from …

Hepatitis C Screening And Treatment Of Prisoners: Analysis Of Policy And Practice In Kentucky, Charity Faith Kranz

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

INTRODUCTION

The United States and the Commonwealth of Kentucky are currently overwhelmed by a triad of complex epidemics—incarceration, opioid overdose deaths secondary to substance use disorders, and hepatitis C. Research has suggested hepatitis C screening and treatment of prisoners may be a cost-effective strategy to address the hepatitis C epidemic. Since Kentucky has been particularly impacted by these interrelated health threats, further exploration of hepatitis C in Kentucky prison populations and their potential role in addressing these epidemics is warranted.

Primary Objective

The main goal of this research was to examine hepatitis C screening and treatment policy and practice within …