Digital Commons Network^™

High Moisture Ryegrass Preservation With Ammonia, L R. Verma, B D. Nelson, C R. Montgomery

IGC Proceedings (1977-2023)

The high humidity in southeast Louisiana prohibits curing of rycgrass hay at its optirnu�� quality st��ge . Oft���� up to 10 days are required in curing rye grass cut before mid-May t':' reach a safe level ��or storage. R��mfall and ��•gh hunudity dunng_ this period increase the curing time and decrease the quality of rye?rass. Bali��g .of !ug�� mo1st':1re hay 1s one way to reduce the curing time and limit the weather related damage. However, microbial _ activity m high moisture hay reduces _its quahty_ due to loss of protein and energy and the formation of molds. Microbial activity may …

Flavor Constituents Of Italian Ryegrass And Rhodes Grass Hays, T All, M Yonaga, H Tanaka

IGC Proceedings (1977-2023)

SUMMARY In connection with the effect of flavors on feed intake and pasture selection by cattle and insect pests, the flavor components of Italian ryegrass (Lolium multiflorum Lamark) and Rhodes grass (Chloris gayana) were isolated by trapping ofheadspace volatiles on Tenax-GC and by steam distillation and identified by gas chromatography and gas chromatography-mass spectrometry. Both fresh grasses have a flavor very similar to odors of (Z)-3-hexen-1-ol and (Z)-3-hexenyl acetate. In the volatiles from the hays, however, the acetate was not detected and the amount of (Z)-3-hexen-1-ol was small, while aldehydes such as 2,4-hexadienal and 2,4-heptadienal were found along with volatile …

Physiological Effect Of Feeding With The Leguminous Shrub, Leucaena Leucocephala, On The Vole, Microtus Arvalis Pallas, And Mice, T Asada, T Arai, T Konno, Y Oki, K Ogimoto

IGC Proceedings (1977-2023)

The leguminous grass, Leucaena leucocephala, is a perennial plant rich in protein and vitamins. An experiment was carried out to obtain basic information required for the practical application of the grass to livestock feeding. In it, herbivorous voles of Microtus arvalis pallas and mice were fed, for 30 days, two basal diets of different composition to which the grass had been added to such extent that the mimosine content of the resulting diets might be about 1 %. As a result, an increase in body weight at the end of the experimental period was more remarkable in voles fed …

Influence Of Supplementation On Herbage Digestibility And On Faeces Characteristics With Sheep, M Chenost, Elisabeth Grenet, C Demarquilly, C Beranger

IGC Proceedings (1977-2023)

The objective of this study is to determine whether or not supplements e.g. concentrates, added at pasture modify the relationship between organic matter digestibility (OMD) of grazed herbage and faecal crude protein content (FCP). This would provide an answer whether or not the OMD of the fraction of herbage in the ration can be predicted from the FCP content of the faeces corresponding to the ration. We have utilized results of 2 digestibility measurements trials in which sheep were fed ad libitum herbage alone or supplemented either with barley (trial 1) or with a concentrate (trial 2). The supplementation leads …

A Simulation Model Of The Seasonal Pattern Of Herbage Digestibility And Its Interaction With Intensity Of Defoliation, A W. Illius

IGC Proceedings (1977-2023)

Despite the considerable efforts expended in studying the seasonal changes in the digestibility of herbage under different harvesting regimes, there has only been slow progress in the modelling of these processes. In this paper, a quantitative description of the factors underlying seasonal changes in herbage digestibility is developed and applied to sets of data from S24 and S23 perennial ryegrass. A new variable, relative maturity, is used to describe the effect of defoliation in interrupting the process of tiller maturation which leads to the decline in digestibility. For S24, the model explained 95.5% of variation in digestibility decline, allowing accurate …

The Effect Of Silage Quality And Level Of Concentrate On The Dry Matter Intake And Milk Production Of British Friesian Heifers, R H. Phipps, J A. Bines, J Thomas, A Cooper, R J. Wilkins

IGC Proceedings (1977-2023)

Trials have shown that both silage quality and level of concentrate are major factors influencing dry matter (DM) intake and milk production with adult dairy cows but there are few data relating to heifers.Seventy-two British Friesian heifers were divided into two groups during weeks 3-12 of lactation and received ad Iibitum either good quality (G) or poor quality (P) grass silage. The in vitro digestible organic matter in the dry matter was 68 and 60 for silages G and P, respectively. In addition, the heifers in each group consumed concentrate at either a low (4.3 kg DM/day), medium (6.4 kg …

Intake Of Grassland - Hay By Dairy Cows And Its Potential Meet Requirements Of Maintenance And Milk Production, J P. Dulphy, C Beranger, D Micol

IGC Proceedings (1977-2023)

There have been few recent studies on the use of grass-hays by dairy cows, probably as a result of the importance given to studies on silage. Therefore 9 grass-hays, harvested in good conditions, were prepared and fed to dairy cows. With a CP content of 133 g/kg DM and an O.M. digestibility of 64.4%, the intake of these hays was 2.07 % of live weight (12.65 kgDM/animal/day). Their energy supply was sufficient to cover the maintenance needs of the cows, and a mean theoretical daily production of 8.1 kg of milk. The real production was 20.1 kg, and the difference …

Barley And Protected Protein Concentrate As Supplements To Timothy Silage For Growing Bulls, H S. Hakkola

IGC Proceedings (1977-2023)

The objective of the experiment was to study the utilization of grass silage by growing bulls. Between the age of 68-434 days, 63 bulls took part in an experiment in which as supplements to timothy silage were 1) barley 2) barley+ protected protein concentrate and 3) protected protein concentrate only. The effects on· silage intake and live weight gain of the bulls were measured. In each feeding group there were 21 bulls. The concentrate contained 30% digestable crude protein of which 72% from soya-bean meal, 18% from rapeseed meal and 10% from milk powder. The protein of the concentrate was …

Evaluation Of Forage At The Level Of Voluntary Intake, M Okamoto, K I. Sung, N Hasegawa, N Yoshida

IGC Proceedings (1977-2023)

To revise the conventional method of forage evaluation, digestion trials were conducted at the level of voluntary intake. Dry matter (DM), crude protein and Neutral Detergent Fibre (NDF) contents in offered and residual feeds were measured, using suffolk wethers which were fed with grass silage and hay. A lower content of crude protein and a higher content ofNDF in residual feeds were detected when compared with those of offered feeds. Then, crude protein and NDF values of intake feeds were calculated from the composition of offered and residual feeds. It was suggested that the reason for different contents of crude …

The Effects Of Heat Treatment And Ruminal Degradation On Digestibility Of Rumen- Bypassed Protein And Total Digestibility, Y Maeda, T Masuko, K Awaya, K Sugimura

IGC Proceedings (1977-2023)

In order to investigate the protein digestibility of heated roughage, the effects of the extent of heat treatment and ruminal degradation on the post-ruminal digestibility of the rumen-bypassed protein and the total digestibility from rumen to post-ruminal tracts were studied. First cutting Italian ryegrass dried at four levels of temperature (freeze, 40, 80 and 120°C) were prepared. Materials were put into the rumen by nylon bag technique for 0, 2 and 6 hr., after rumen exposure, the residual protein was incubated by pepsin-pancreatin artificial digestion method. The total protein was partitioned using three fractions based on ruminal and pepsin-pan-creatin (P-P)degestibility. …

An Expert Nirs Forage System, John S. Shenk, M O. Westerhaus

IGC Proceedings (1977-2023)

Near infrared spectroscopy (NIRS) has become an accepted technique to analyze the nutritive value of forage and feedstuffs. Recent advancements have been made in developing universal calibration equations that can be transferred from instrument to instrument. This advancement has made it possible to use NIRS as the analytical component of expert system software in conjunction with livestock feeding models. The software is flexible to provide information for research, extension, and industry applications. The incorporation of NIRS into an expert system promises to make possible new advancements in efficiency of livestock production.

Near Infrared Reflectance Spectroscopy Analysis Of Quality Of Farm- Grown Alfalfa, N P. Martin, G C. Marten, D A. Schriever, J L. Halgerson, F R. Ehle

IGC Proceedings (1977-2023)

Successful use of near infrared reflectance spectroscopy (NIRS) analysis of crude protein (CP), acid detergent fiber (ADF) and neutral detergent fiber (NDF) of farm-grown alfalfa (Medicago sativa) is described. Calibration equations for CP, ADF, and NDF using the BEST/NeoTech 6100 IR spectro-computer system1 were developed from windrow alfalfa samples collected throughout Minnesota over three seasons. Standard errors of calibration were 0.57, 1.56 and 1.83 dag/kg for CP, ADF and NDF, respectively. Coefficients of multiple determination (R2) were 0.97, 0. 91 and 0. 93, respectively. Standard errors of NIRS analysis for CP, ADF, and NDF were 0.58, 1.48 and …

Development Of A National Alfalfa Quality Assessment Procedure In The United States, V L. Marble, W C. Templeton

IGC Proceedings (1977-2023)

Only a small fraction of the U.S. hay crop is evaluated for nutritive properties. Various states have developed alfalfa hay quality prediction systems based on chemical analysis. Confusion among users has resulted from nonuniformity among systems. Additional factors causing concern include: increased awareness among producers of higher income from the sale of high quality hay; increased demand among dairymen for a prediction system compatible with ration formulation; recognition of the inadequacy and nonuse of U.S. federal hay grades; increased movement of alfalfa hay across state borders; and lack of agreement on duplicate samples sent to different laboratories. After formation of …

The Cultivation And Feeding Value Of Persian Clover, P C. Flinn, K.F M. Reed, G R. Saul, G N. Ward, J G. Graham

IGC Proceedings (1977-2023)

Research with Persian clover (Trifolium resupinatum, ssp. majus) cv. Mara!, in south eastern Australia shows it to be productive and well adapted to waterlogged soils. It is particularly productive in spring when compared with other clovers. Winter production can be increased by sowing Persian clover with Westerwolds ryegrass or oats but the yield of clover in the spring is therby reduced. Persian clover conserved as hay was found to have a low neutral detergent fibre content (28.1 % ). In a 60 day feeding trial, lambs were fed Persian clover hay (78.5% digestible dry matter), lucerne hay (63.0% …

Voluntary Intake And Digestibility Of Two Selected Tropical Grasses By Swamp Buffaloes, M Wanapat, A Topark-Ngarm

IGC Proceedings (1977-2023)

A study on chemical compositions, voluntary feed intake and nutrient digestibility of Panicum maximum cv. Guinea and Brachiaria decumbens cv. Signal at three regrowth cuttings (week 4, 8, 12) by swamp buffalo steers, was conducted. Dry matter, acid detergent lignin contents increased whereby ash, crude protein and neutral detergent fiber contents decreased, as the plants matured. Acid detergent fiber fraction remained similar for all cuttings. Voluntary dry matter intake and digestion coefficients of nutrients were relatively high at week 4 and 8 and markedly low at week 12 for both species. Buffaloes fed with signal grass had higher intakes and …

Research For Selection Criteria For Grass Quality, Based Upon Animal References, M Gillet, J Scehovic, M Lila

IGC Proceedings (1977-2023)

In an effort to find selection criteria for intake and digestibility, based on correlations with the same characters measured on animals, we have compared 4 to 6 grasses of similar heading dates for intake, palatability, digestibility by sheep, intake by goats and many laboratory tests. 1979 results were published for Lexington Congress. Results of 1980 and 1981 springs bear them out in several points :

- palatability was a very precise and stable test

- palatability, digestibility and goat intake were stable and correlated with each other

- soluble phenols were able to predict palatability and part of digestibility

-sheep …

Using Grinding Energy Measurements For Predicting Crude Fibre Content In First Growths Of Permanent Grassland, Chr Paul, U V. Borstel, E Zimmer

IGC Proceedings (1977-2023)

The performance of a grinding energy test for the simple and rapid prediction of crude fibre content in spring growth of grassland was investigated during three successive years and its potential for deriving recommendations on appropriate cutting dates demonstrated. The grinding energy test appeared to achieve a precision comparable to that for near infrared based predictions of crude fibre reported from the literature. Prediction bias consisting in a mean 2% crude fibre underestimation was found to occur in one year presumably due to an anomalous _constellation of environmental conditions. This appeared to have affected the pattern of plant development and …

Cell Wall Composition And Rate Of Digestion Of Brown-Midrib Sorghum Internodes As Influenced By Maturity, J H. Cherney, J J. Volenec, K J. Moore

IGC Proceedings (1977-2023)

More information is needed on the growth of secondary plant cell walls in forage species, and the relationship of cell wall structure to rate and extent of digestion. Our overall objective was to relate changes in cell wall composition with maturation to rate and extent of cell wall digestion. The seventh intemode of normal and brown-midrib ( bmr) sorghum (Sorghum bicolor (L.) Moench.) was harvested at eight maturities beginning with the cessation of intemode elongation. Dry weight per segment was determined for each maturity stage. Lignin accumulation in lntemode 7 increased during maturation, with the rate of lignin synthesis being …

The Effect Of The Species, Stage Of Maturity And Level Of Intake Of Grass On The Efficiency Of Microbial Protein Synthesis In The Rumen Of Cattle, F J. Hart, Jane Leibholz

IGC Proceedings (1977-2023)

This study was undertaken because there is a paucity of information in the literature on the efficiency of microbial protein synthesis in the rumen of cattle when grass is fed as the sole diet. There is also little experimental data available on the factors which affect the efficiency of microbial protein synthesis. The work involved feeding six rumen fistulated Friesian steers with three species of grass which were harvested at various tages of maturity. In addition each maturity of forage was fed at three 15levels of orp5 nic matter ( OM) intake.

as sampled at the omasum and microbial protein …

The Influence Of Aging On Forage Quality Of Individual Switchgrass Leaves And Stems, B Anderson

IGC Proceedings (1977-2023)

Cattle selectrively graze switchgrass (Panicum virgatum L.) tops prior to the boot stage and prefer vegetative culms and new tillers following heading. Therefore, a study was conducted to evaluate the changes in forage composition of individual leaves and stems during growth to determine if grazing selection may be influenced by aging. Plants were cut periodically at ground level from collaring of the first leaf until anthesis. Leaves (leaf blade plus sheath) of different ages, stems, and the whorl were hand separated at each harvest. Crude protein, in vitro dry matter disappearance (IVDMD), neutral and acid detergent fiber, lignin, and …

Harvesting Alyceclover For Maximum Dry Matter Yield And Nutrient Value, D D. Baltensperger, W G. Blue, W H. Anderson

IGC Proceedings (1977-2023)

Limited information is available on the quality of alyceclover (Alysicarpus vaginalis) in relation to plant stress. This study was undertaken to clarify the relationship between plant maturity and forage yield, quality and mineral composition in order to optimize each at harvest. Alyceclover was grown for two summers and new plots were harvested weekly through the pod-fill stage of development beginning eight weeks after planting. Samples were analyzed for dry matter, organic matter, In vitro dry matter digestibility, nitrogen, phosphorus, potassium, calcium, magnesium, manganese, copper, iron and zinc. Dry matter yields increased through the flowering stage of development except …

Adaptation Of The Inra System Of Determination And Expression Of The Nutritive Value Of Forages To The Tropical Conditions In The Caribbean Island, A Xande, R Garcia-Trujillo

IGC Proceedings (1977-2023)

This program have undertaken a series of trials of systematic measure of nu tritive value on the tropical forages. The choice of the calculation and of the way of expression of energetic and nitrogen values required comparation measures in different systems as well as attempts in alimentation before adopting the new french INRA system of energy (UFL-UFV) and of the protein value (POI). The general results of this study is a preamble to the diffusion of the table of nutritive value of the tropical forages. For this purpose 450 samples of forages have been analysed on sheep in cage of …

Protein Structure Influences Redox Stability, Oxygen Affinity, And Heat-Induced Denaturation Properties Of Bovine Myoglobin, Hemoglobin, And Cytochrome C, Anuj Sharma, Runnan Li, Silan Bhandari, Surendranath P. Suman, Sadagopan Krishnan, Morgan Pfeiffer, Gretchen Mafi, Ranjith Ramanathan

Animal and Food Sciences Faculty Publications

While past research has predominantly focused on the role of myoglobin in meat color, investigations into the contributions of hemoglobin and cytochrome c are limited. Cytochrome c has the highest redox stability (P < 0.05), followed by myoglobin and hemoglobin. Electrochemistry analysis revealed that cytochrome c has a greater capacity (P < 0.05) to reduce than myoglobin and hemoglobin. Differential scanning calorimetry indicates that cytochrome c was most stable to heat (P < 0.05), while no differences were noted between myoglobin and hemoglobin. Structural analysis noted that greater heat and redox stability of cytochrome c might be attributed to fewer histidine residues (3) and more covalent bonds (4) than myoglobin (13), hemoglobin α (10), and hemoglobin β (6). This study suggests that the amino acid sequence and number of covalent bonds can impact the redox stability and heat-induced denaturation properties of myoglobin, hemoglobin, and cytochrome c.

The International Soft Tissue Sarcoma Consortium, Gianni Bisogno, Monika Sparber-Sauer, David Rodeberg, Johannes H.M. Merks, Ewa Koscielniak, Suzanne L. Wolden, Gian Luca De Salvo, Paola Del Bianco, Wei Xue, Martin Ebinger, Christian Vokuhl, Rajkumar Venkatramani, Samuel L. Volchenboum, Brian Furner, Veronique Minard Colin, Douglas S. Hawkins

Surgery Faculty Publications

Background: The International Soft Tissue Sarcoma Consortium (INSTRuCT) was established in 2017 to enhance international collaboration. This study describes the characteristics of rhabdomyosarcoma (RMS) patients in the INSTRuCT database and examines differences across contributing groups. Methods: INSTRuCT includes data Children’s Oncology Group (COG), Cooperative Weichteilsarkom Studiengruppe (CWS), and European Paediatric Soft Tissue Sarcoma Study Group (EpSSG) along with prior studies from Malignant Mesenchymal Tumour Committee (MMT) and Italian Soft Tissue Sarcoma Committee (STSC). Data standardization was supported by the University of Chicago’s Pediatric Cancer Data Commons. Pseudonymized patient-level data from clinical trials were harmonized. Differences across groups were assessed using …

The Kentucky Microbiome Dataset, Rachel L. Washburn, Douglas C. Curl

Research Data--KGS

The Kentucky Microbiome Dataset is a continuously updated geolocated dataset which describes the distribution and diversity of microorganisms across the Commonwealth of Kentucky. Each geolocated point represents a location reported in the peer-reviewed literature where microorganisms were sampled from soil, water, geologic substrates, or clinical settings. Environmental studies include only non-host-associated, non-clinical microorganisms sampled from natural environments. Clinical studies are mapped separately to highlight locations where investigations into host-associated or human health-related microbes have occurred.

For each location, associated metadata include study information, taxonomic classifications, sample type, sampling method, latitude and longitude, sampling date, publication reference, and a direct link …

Who Reconstitutes The Chemotherapy? The Educational And Practice Needs Of Oncology Pharmacists In West Africa: A Call For Multidisciplinary Team Approach In Oncology Care., Runcie C.W. Chidebe, Ramatu M. Alabelewe, Krishna Prasad Sapkota, Darlingtina K. Esiaka, Candidus C. Nwakasi, Gloria C. Okwu, Jacinta Emejulu, Adamu A. Umar, Ndifreke Okwuegbunam, Funsho David, Toyosi Adepoju, Agha A. Agha, Ejike S. Ugwu, Simeon C. Aruah, Richard Mshelia, Rob Duncombe, Noel N. Wannang, Peace C. Babalola, R. Donald Harvey, Cindy O'Bryant, Folakemi T. Odedina

Behavioral Science Faculty Publications

PURPOSE: Oncology pharmacists (OPs) play a crucial role in cancer care, treatment, survivorship, and multidisciplinary teams (MDTs). OPs have specialized training in designing, administering, monitoring, and modifying oncology chemotherapy; managing adverse events; and evaluating clinical trials and investigational drugs. Yet, the state of OP has remained largely unknown in the clinical oncology workforce of the West African region. Therefore, this study aimed to understand who reconstitutes chemotherapy and to explain the OP educational and practice needs, challenges, and solutions in Nigeria.

MATERIALS AND METHODS: Using a concurrent embedded mixed method design, 35 OPs completed a questionnaire, and 12 others responded …

Endothelium- And Fibroblast-Derived C-Type Natriuretic Peptide Prevents The Development And Progression Of Aortic Aneurysm, Aisah A. Aubdool, Amie J. Moyes, Cristina Perez-Ternero, Reshma S. Baliga, Jaspinder Singh Sanghera, M. Taaha Syed, Kareemah Jaigirdar, Anmolpreet K. Panesar, Janice C. Tsui, Yanming Li, Hernan G. Vasquez, Ying H. Shen, Scott A. Lemaire, Juliette Raffort, Ziad Mallat, Hong S. Lu, Alan Daugherty, Adrian J. Hobbs

Physiology Faculty Publications

BACKGROUND: Thoracic (TAA) and abdominal (AAA) aortic aneurysm are life-threatening diseases characterized by dilation, inflammation, and structural weakness; development of pharmacological therapies is desperately needed. CNP (C-type natriuretic peptide) plays a key role in vascular homeostasis, mediating vasodilator, anti-inflammatory, and antiatherogenic actions. Since such processes drive AA, we determined the role of endogenous CNP in offsetting pathogenesis. METHODS: Tissue from patients with AA was analyzed to determine the consequences on CNP signaling. Ascending and suprarenal aortic diameters were assessed at baseline and following Ang II (angiotensin II; 1.44 mg/kg per day) infusion in wild-type, endothelium-restricted (ecCNP^-/-), fibroblast-restricted (fbCNP …

At The Nexus Between Epigenetics And Senescence, Toby L. Chambers, Jaden Wells, Pieter Jan Koopmans, Francielly Morena, Zain B. Malik, Nicholas P. Greene, Antonio Filareto, Michael Franti, Patrizia Sini, Harald Weinstabl, Robert T. Brooke, Milda Milčiūtė, Juozas Gordevičius, Steve Horvath, Yuan Wen, Cory M. Dungan, Kevin A. Murach

Physiology Faculty Publications

Senescent cells emerge with aging and injury. The contribution of senescent cells to DNA methylation age (DNAmAGE) in vivo is uncertain. Furthermore, stem cell therapy can mediate “rejuvenation”, but how tissue regeneration controlled by resident stem cells affects whole tissue DNAmAGE is unclear. We assessed DNAmAGE with or without senolytics (BI01) in aged male mice (24–25 months) 35 days following muscle healing (BaCl₂-induced regeneration versus non-injured). Young injured mice (5–6 months) without senolytics were comparators. DNAmAGE was decelerated by up to 68% after injury in aged muscle. DNAmAGE was modestly but further significantly decelerated by injury recovery with …

Carbohydrate Metabolism In The Intercaruncular Endometrium Is Affected By Form Of Supplemental Selenium At Maternal Recognition Of Pregnancy In Beef Heifers, Sarah N. Carr, Benjamin R. Crites, Kwangwon Son, Phillip J. Bridges

Animal and Food Sciences Faculty Publications

Selenium (Se)-deficient soils result in Se-deficient forages that do not meet the nutritional requirement for this trace mineral in grazing cattle. Supplementation of Se conventionally occurs through a basal vitamin/mineral mix containing Se in an inorganic form (ISe), although organic Se (OSe) is available when cattle graze on forage. We previously reported that the provision of supplemental Se as a 1:1 mixture of ISe:OSe (MIX), in contrast to the industry standard of ISe alone, increases early luteal phase concentrations of systemic progesterone (P4) and increases the length of the conceptus at day 17 of pregnancy. The objectives herein were to …

Genetically Proxied Blood Pressure, Vascular Brain Injury, And Alzheimer's Disease Pathology, Iyas Daghlas, Michelle R. Caunca, Lincoln M.P. Shade, Malik Nassan, Kristine Yaffe, David W. Fardo, Dipender Gill

Biostatistics Faculty Publications

INTRODUCTION: Lower blood pressure (BP) is linked to reduced dementia risk, though it is uncertain whether this benefit stems solely from mitigating vascular brain injury (VBI) or also extends to directly influencing Alzheimer's disease (AD) pathology. We leveraged Mendelian randomization (MR) to assess whether lifelong lower BP is causally associated with neuropathological correlates of VBI and AD. METHODS: We identified genetic proxies for systolic and diastolic BP (n = 1,028,980) and applied them in MR analyses of post mortem neuropathological measures of VBI and AD (n = 6363–7786). RESULTS: Genetically proxied lower systolic BP associated with reduced risk of all …