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The Effect Of An Employee Recognition Program For Clinical Registered Nurse Retention At Eastern State Hospital, Kristi Richmond Jan 2024

The Effect Of An Employee Recognition Program For Clinical Registered Nurse Retention At Eastern State Hospital, Kristi Richmond

DNP Projects

Abstract

Background: Nurse executives want to build and retain teams capable of delivering high quality care. However, registered nurse (RN) turnover continues to jeopardize this goal. RN satisfaction has been associated with reduced turnover in the nursing field. Adequate staffing levels are linked to better patient outcomes, and employee recognition is a key factor in RN retention.

Purpose: The purpose of this study was to evaluate the effectiveness of utilizing an existing recognition tool for RNs at an inpatient state psychiatric hospital in the southeastern United States to determine level of job satisfaction, level of job loyalty, level of engagement, …


Improving Dental Students' Knowledge And Confidence In Treating Tobacco Use, Victoria Pierce Jan 2024

Improving Dental Students' Knowledge And Confidence In Treating Tobacco Use, Victoria Pierce

DNP Projects

Background: Tobacco use can lead to numerous chronic health conditions. Healthcare professionals in the dental field are in a unique position to broach this issue with patients. Research shows that dental students do not feel equipped to provide tobacco cessation care, citing lack of education on the subject among their top reasons for this.

Purpose: The purpose of this project was to evaluate changes in dental students’ knowledge and confidence related to treating tobacco use after participation in an educational intervention about the 5A’s, behavioral counseling, and first-line prescription medications.

Methods: This quality improvement initiative utilized a quasi-experimental survey design …


Improving Nutrition Screening Practice In The Hospitalized Heart Failure Patient Population, Amanda J. Bourgeois Jan 2024

Improving Nutrition Screening Practice In The Hospitalized Heart Failure Patient Population, Amanda J. Bourgeois

DNP Projects

Background: Heart Failure is a disease known to affect nearly 6.5 million adults in the United States. Characterized by recurrent hospitalizations, heart failure significantly contributes to morbidity, mortality, and healthcare costs in the United States and worldwide. Because malnutrition is prevalent in the heart failure population, healthcare providers must perform nutritional assessments on admission to intervene in the case of malnutrition, prevent deterioration, and improve patient prognosis. Without intervention and early identification of malnutrition, heart failure hospitalizations will remain a significant problem.

Purpose: The purpose of this study is to evaluate the effect of an evidence-based educational program for cardiac …


An Educational Intervention On Provider Knowledge Of Hypertension Guidelines And Home Blood Pressure Monitoring, Alison Claire Oesterritter Jan 2024

An Educational Intervention On Provider Knowledge Of Hypertension Guidelines And Home Blood Pressure Monitoring, Alison Claire Oesterritter

DNP Projects

Background: While hypertension is the leading preventable risk factor for cardiovascular disease, disease control remains suboptimal. In the U.S., 47% of adults have hypertension, but only 25% are controlled. Despite having quality evidence-based guidelines, many recommendations are not implemented due to clinical inertia, or the tendency to not change therapy when targets aren’t met. Research has shown that this can be overcome with educational programs. Purpose: To provide training on hypertension guidelines and home blood pressure monitoring (HBPM) to overcome clinical inertia and improve patient outcomes related to hypertension. Methods: The project was a quasi-experimental, pre- and post- …


Improving Knowledge, Confidence, And Competency Among Icu Nurses Concerning The Benefits Of Using The Uk Ventilator Separation Protocol, Brittany Monroe Jan 2024

Improving Knowledge, Confidence, And Competency Among Icu Nurses Concerning The Benefits Of Using The Uk Ventilator Separation Protocol, Brittany Monroe

DNP Projects

Background: The trauma intensive care unit (ICU) at University of Kentucky (UK) Healthcare uses a ventilator separation protocol which provides specific guidelines on the successful weaning of patients from mechanical ventilation. However, many of the nurses are not aware of this protocol as formal education is not included in their orientation or training. This lack of knowledge can lead to risks such as increased patient morbidity, mortality, ICU length of stay, and healthcare-related costs. Research shows that using structured mechanical ventilator weaning tools in the ICU can shorten the duration of mechanical ventilation, which in turn, lowers these associated risks. …


The Impact Of Lung Cancer Screening Education On Knowledge And Screening Rates In A Kentucky Cancer Survivorship Clinic, Alexandra Isler Jan 2024

The Impact Of Lung Cancer Screening Education On Knowledge And Screening Rates In A Kentucky Cancer Survivorship Clinic, Alexandra Isler

DNP Projects

Background: Lung cancer is a significant problem in Kentucky, which ranks fourth in the nation in adults who currently smoke. Early detection, through lung cancer screening, can prevent untimely deaths. Several barriers to screening exist including lack of knowledge and awareness among patients regarding lung cancer screening. By increasing knowledge, at risk patients can take the necessary steps to decrease incidence of late stage diagnoses.

Purpose: The purpose of this project was to evaluate patients’ knowledge of lung cancer screening and referral and screening rates before and after one-on-one patient education.

Methods: This was a quality improvement project that used …


Implementation Of An Education Intervention Aimed At Intensive Care Unit Providers To Improve Knowledge Of And Screening For Cognitive Impairment Related To Post-Intensive Care Syndrome, Terry Harrod Jan 2024

Implementation Of An Education Intervention Aimed At Intensive Care Unit Providers To Improve Knowledge Of And Screening For Cognitive Impairment Related To Post-Intensive Care Syndrome, Terry Harrod

DNP Projects

Abstract

Background: Post intensive care syndrome is a condition that can lead to a range of physical,

cognitive, and psychiatric issues and is frequently undiagnosed. Educating Nurse Practitioners,

Physician Assistants, and Physician Fellows who work in the ICU about post intensive care

syndrome and a cognitive screening tool like the MoCA can significantly enhance their

understanding and ability to diagnose a component of this syndrome.

Purpose: The purpose of this project was to evaluate the effect of an educational intervention

regarding post intensive care syndrome among ICU nurse practitioners, physician assistants, and

physician fellows treating patients within the ICU.

Methodology: …


Provider And Staff Education Surrounding Universal Hcv Screening And Linkage To Care In An Ambulatory Care Setting, Lauren Clifford Jan 2024

Provider And Staff Education Surrounding Universal Hcv Screening And Linkage To Care In An Ambulatory Care Setting, Lauren Clifford

DNP Projects

Abstract

Background: In the United States, it is estimated that there are more than 2 million cases of Hepatitis C (HCV), with over 66,000 new cases since 2020 However, an estimated 75% of those with HCV go undiagnosed and less than 35% receive treatment within a year of diagnosis. In 2018, UK Healthcare (UKHC) implemented a universal HCV screening method using a Best Practice Advisory (BPA) for anyone 18 and older who presented to the Emergency Department and had labs ordered. Patients who screened positive were then referred to outpatient clinics for HCV treatment. Approximately ten percent of positive patients …


The Effects Of Yoga On Anxiety And Sleep Quality Levels Among College Students, Larisa Lucas Mullins Jan 2024

The Effects Of Yoga On Anxiety And Sleep Quality Levels Among College Students, Larisa Lucas Mullins

DNP Projects

Background: College students have poor sleep quality due to a newfound sense of freedom, exposure to new influences, or poor sleep hygiene (Dinis & Barganca, 2018). Specifically, evidence supports a correlation between sleep latency in onset or lack of nighttime sleep and increased anxiety (Dinis & Barganca, 2018). Yoga has been shown to decrease anxiety, stress symptoms, and depression (Mullen et al., 2021). Teaching yoga techniques and encouraging routine yoga practice to college students may improve sleep quality and decrease anxiety.

Purpose: This project's purpose was to evaluate the effect of yoga education and videos on sleep quality and …


Electroconvulsive Therapy: Improving Psychiatric Rn Knowledge And Attitudes Using An Educational Module, Garrett Payne Jan 2024

Electroconvulsive Therapy: Improving Psychiatric Rn Knowledge And Attitudes Using An Educational Module, Garrett Payne

DNP Projects

Abstract

Background: Electroconvulsive Therapy (ECT) is a robust, versatile treatment option for many psychiatric disorders and diseases, but systemic low levels of knowledge and negative attitudes exist towards the procedure among healthcare workers, specifically among psychiatric RNs. The negative attitudes surrounding ECT are most attributable to a misunderstanding of its uses, efficacy, procedures, and safety. Psychiatric RNs hold more negative beliefs and misconceptions about ECT than any other group of healthcare workers who directly care for patients receiving the treatment. Psychiatric RNs also demonstrate poor understanding of ECT treatment procedures and outcomes. Current evidence-based practice shows that providing psychiatric RNs …


Nurse Management Competency Training For Frontline Nurse Leaders, Travis Drake Jan 2024

Nurse Management Competency Training For Frontline Nurse Leaders, Travis Drake

DNP Projects

Background: Growing evidence shows that front line nurse leaders take on their role with minimal education and training. Healthcare facilities are pressed for resources and time to allow for proper development of these leaders.

Purpose: The purpose of this project was to provide novice nurse leaders with a condensed management development program utilizing key American Organization of Nurse Leaders (AONL) nurse manager competencies. This abbreviated program was developed focusing on three key competencies of finance, innovation, and human resources. The impact of the program was measured by comparing self-assessment prior to any of the education and after the entire program …


An Educational Intervention To Improve Neonatal Icu Nurses’ Breastfeeding Support Knowledge, Self-Efficacy, And Breastfeeding Rates At Discharge, Sarah Money Jan 2024

An Educational Intervention To Improve Neonatal Icu Nurses’ Breastfeeding Support Knowledge, Self-Efficacy, And Breastfeeding Rates At Discharge, Sarah Money

DNP Projects

Background: The benefits of breastfeeding for both mother and baby are known. It is recommended that all infants be exclusively breastfed until six months (Meek & Noble, 2022; North et al., 2021). Despite this, breastfeeding rates remain low, especially in medically fragile infants requiring care in the neonatal intensive care unit (NICU) (Rollins et al., 2016). Receipt of evidence-based breastfeeding support by healthcare providers has been shown to improve breastfeeding success; however, many healthcare providers report not being comfortable or knowledgeable enough to offer support consistently (Mulcahy et al., 2022; Rollins et al., 2016).

Purpose: This study aims …


Utilizing Mixed Methods To Examine Black Graduate Students’ Experiences With Multicultural Supervision In School Psychology, Larraine Graham Jan 2024

Utilizing Mixed Methods To Examine Black Graduate Students’ Experiences With Multicultural Supervision In School Psychology, Larraine Graham

Theses and Dissertations--Educational, School, and Counseling Psychology

Multicultural supervision is essential in preparing psychologists-in-training to provide ethical, multiculturally competent practices to diverse populations. In the field of school psychology, Black graduate students remain underrepresented despite increasing diversity in schools. While there are many resources for establishing best practices in multicultural supervision in related fields, there is a lack of literature in school psychology, especially that examines Black graduate students’ experiences. Therefore, the purpose of the current study is to attend to the gap in the literature and better understand Black graduate students’ experiences with the supervisory relationship, satisfaction with supervision, and multicultural competency skill development. The current …


Building Children's Connection To Nature In The Schools: A Piloted Nature-Based Intervention, Alexis Bird Jan 2024

Building Children's Connection To Nature In The Schools: A Piloted Nature-Based Intervention, Alexis Bird

Theses and Dissertations--Educational, School, and Counseling Psychology

Nature-based interventions (NBI) have demonstrated positive effects on a range of student outcomes, including enhanced prosocial behaviors, decreased levels of stress, and improved mood and concentration. Further, school-aged children’s participation in NBIs supports students’ reconnection with the natural world. Although schools represent an ideal setting to engage students from diverse backgrounds in NBI activities, existing research is limited in understanding the effects and feasibility of integrating NBIs within school settings. This study aimed to explore the effects of NBI activities administered within a school program on students’ sense of connectedness with nature and their overall wellbeing. Additionally, participants were interviewed …


Exploring The Effect Of Casting Solution Composition, Additives, And Casting Conditions On The Morphology And Separation Performance Of Pvdf Membranes, Rachel Kaplan Jan 2024

Exploring The Effect Of Casting Solution Composition, Additives, And Casting Conditions On The Morphology And Separation Performance Of Pvdf Membranes, Rachel Kaplan

Theses and Dissertations--Chemical and Materials Engineering

Global water scarcity and quality concerns in the past decade have led to a looming global water crisis. Polymeric membranes have emerged as a potential solution due to their modularity, operational reliability, and wide range of contaminant removal. Polyvinylidene fluoride (PVDF) is often selected as a membrane material for its enhanced chemical resistance, thermal stability, and mechanical strength. However, these membranes often require more complex fabrication methods or support materials to achieve optimum performance. Flat-sheet unsupported PVDF membranes were fabricated via nonsolvent induced phase separation. The influence of solvent, polymer composition, pore forming additives, and casting conditions were explored with …


Sustained Release Systems For Endometrial Cancer, Claire E. Rowlands Jan 2024

Sustained Release Systems For Endometrial Cancer, Claire E. Rowlands

Theses and Dissertations--Chemical and Materials Engineering

Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is the most common cancer of female reproductive organs. From 2007-2016 there has been a global rise in incidence of EC of 1.3 %. Typically, EC is treated with surgery, but at later stages surgery is less effective requiring chemotherapy and/or radiation therapy. These treatment options are less effective than surgery and there are very few FDA approved chemotherapies for EC. The minuscule number of effective treatment options as well as an increase in incidence shows the need for creating improved treatments for EC. There has been …


Addressing The Effects Of Counterion Size On The Properties Of Electrochemically Doped Conjugated Polymers, Kyle Baustert Jan 2024

Addressing The Effects Of Counterion Size On The Properties Of Electrochemically Doped Conjugated Polymers, Kyle Baustert

Theses and Dissertations--Chemistry

Conjugated polymers (CPs) are an emerging class of materials that are attractive for many applications due to their low cost, flexibility, and ease of processing. Both chemical and electrochemical doping of CPs is done to alter important properties such as their electrical conductivities and optical absorbances. This ability to tune their properties allows for their use in devices such as organic electrochemical transistors (OECTs) for biosensing and bioelectronics, electrochromic devices for color changing windows, as well as wearable thermoelectrics to use body heat to power wearable electronics. There are several factors that can influence the properties of doped CPs including …


Synthetic Strategies Toward The Development Of Stable Gold-Based Anticancer Agents And Their Mechanistic Insight, Adedamola Arojojoye Jan 2024

Synthetic Strategies Toward The Development Of Stable Gold-Based Anticancer Agents And Their Mechanistic Insight, Adedamola Arojojoye

Theses and Dissertations--Chemistry

Despite notable advancements in the design and synthesis of novel chemotherapeutic drugs, cancer continues to be a leading cause of death in the United States. Metallodrugs, especially Au complexes, have played a prominent role in the search for the next generation of chemotherapeutic agents. Herein, I report on the synthetic strategies towards the rational design of stable Au (Au)-based agents, study their reactivity in biological environment, and offer insights into the cytotoxic mechanism of action in cancer. Chemotherapeutic Au compounds exist mainly in +I or +III oxidation states. Repurposing of the Au(I) FDA approved auranofin for the treatment of diseases …


Fundamental Studies Of Optical Properties And Electrocatalytic Properties Of Low Dimensional Nanomaterials Carbon Nano Dots And 2d Transition Metal Dichalcogenides, Nadeesha Kothalawala Jan 2024

Fundamental Studies Of Optical Properties And Electrocatalytic Properties Of Low Dimensional Nanomaterials Carbon Nano Dots And 2d Transition Metal Dichalcogenides, Nadeesha Kothalawala

Theses and Dissertations--Chemistry

Low dimensional nanomaterials are widely used in a variety of applications such as opto-electronics, energy production and storage, and bio-medical applications due to their unique optical, catalytic, electronic, and mechanical properties. Most of the nanomaterials offer a plethora of modifications in their structure, composition, morphology, and dimensionality that can lead to the manipulation of their physiochemical properties. This dissertation presents insight into two types of compelling nanomaterials: carbon nanodots (CND) and transition metal dichalcogenides (TMD), investigating their optical and electrocatalytic properties respectively.

Carbon nanodots (CND) are a promising class of photoluminescent nanomaterials that hold a significant potential in many optoelectronic …


Investigating The Behavior Of Redox–Active Organic Molecules In Electrochemical Energy Storage Through Experiment–Guided Computational Simulations, Anton Perera Jan 2024

Investigating The Behavior Of Redox–Active Organic Molecules In Electrochemical Energy Storage Through Experiment–Guided Computational Simulations, Anton Perera

Theses and Dissertations--Chemistry

The long–anticipated implementation of using renewable sources such as solar and wind to meet the world’s energy demand is still limited by not having appropriate grid–level energy storage systems on a global scale. When considering grid–scale energy storage solutions, environmental concerns, and techno–economic viability play a crucial role in the practical implementation of such technology. To address this, redox–active organic molecules (ROMs) have been explored for many uses including serving as redox–active molecules in redox flow batteries (RFBs) and providing overcharge protection in lithium–ion batteries (LIBs).

Nevertheless, in order to be a major player in electrochemical energy storage, further optimization …


Impacts Of Key Degradation Byproducts On Amine Scrubbing For Post-Combustion Carbon Capture, Thomas Jorgensen Jan 2024

Impacts Of Key Degradation Byproducts On Amine Scrubbing For Post-Combustion Carbon Capture, Thomas Jorgensen

Theses and Dissertations--Chemistry

The increasing atmospheric CO2 concentration causes significant concern about global warming and its environmental impact. Industrial sectors, including power generation, transportation, and production factories, contribute most of the anthropogenic sources of CO2 emissions. Researchers search for methods to mitigate these CO2 emissions without compromising the benefits of industrial processes, including CO2 capture, utilization, and sequestration (CCUS). Post-combustion carbon capture (PCCC) by amine scrubbing is the most technology ready CCUS, with various pilot-scale projects worldwide showing small-scale to fully operational plants. While researchers have made significant progress in amine scrubbing, they strive to improve the efficiency and …


Disrupted One-Carbon Metabolism In Heifers Negatively Affects Their Health And Physiology, Matthew S. Crouse, Ronald J. Trotta, Harvey C. Freetly, Amanda K. Lindholm-Perry, Bryan W. Neville, William T. Oliver, Carrie J. Hammer, Jessica G. Syring, Layla E. King, Tammi L. Neville, Lawrence P. Reynolds, Carl R. Dahlen, Joel S. Caton, Alison K. Ward, Robert A. Cushman Jan 2024

Disrupted One-Carbon Metabolism In Heifers Negatively Affects Their Health And Physiology, Matthew S. Crouse, Ronald J. Trotta, Harvey C. Freetly, Amanda K. Lindholm-Perry, Bryan W. Neville, William T. Oliver, Carrie J. Hammer, Jessica G. Syring, Layla E. King, Tammi L. Neville, Lawrence P. Reynolds, Carl R. Dahlen, Joel S. Caton, Alison K. Ward, Robert A. Cushman

Animal and Food Sciences Faculty Publications

The objective of this study was to determine the dose-dependent response of one-carbon metabolite (OCM: methionine, choline, folate, and vitamin B12) supplementation on heifer dry matter intake on fixed gain, organ mass, hematology, cytokine concentration, pancreatic and jejunal enzyme activity, and muscle hydrogen peroxide production. Angus heifers (n = 30; body weight [BW] = 392.6 ± 12.6 kg) were individually fed and assigned to one of five treatments: 0XNEG: total mixed ration (TMR) and saline injections at days 0 and 7 of the estrous cycle, 0XPOS: TMR, rumen-protected methionine (MET) fed at 0.08% of the diet dry matter, rumen-protected choline …


Ergot Alkaloid Consumption Alters Serotonin Receptor-Induced Vasoactivity In Ovine Umbilical Vasculature, James L. Klotz, Jessica L. Britt, Maslyn A. Greene, Coral Kent-Dennis, Susan K. Duckett Jan 2024

Ergot Alkaloid Consumption Alters Serotonin Receptor-Induced Vasoactivity In Ovine Umbilical Vasculature, James L. Klotz, Jessica L. Britt, Maslyn A. Greene, Coral Kent-Dennis, Susan K. Duckett

Animal and Food Sciences Faculty Publications

Consumption of ergot alkaloids during the second half of gestation has been shown to decrease umbilical artery vasoactivity resulting in decreased birth weights. Negative vascular effects of ergot alkaloids are mediated predominantly through serotonergic and adrenergic receptors in other tissues. Vasoactivity of serotonin (5-HT) receptors 5-HT2A and 5-HT1B/1D in umbilical artery and vein from ewes receiving endophyte-infected seed (E + 1.77 mg ergovaline/hd/d) or a control total mixed ration (CON; 0 mg ergovaline/hd/d) tall fescue seed at d-110 and d-133 of gestation was evaluated. Gravid reproduction tracts were collected from ewes. Two-mm sections of umbilical artery and vein …


Effect Of Chronic Cyclic Heat Stress And Supplemented Inorganic And Organic Zinc Source Levels On Grow-Finish Pig Growth Performance And Estimated Body Composition, Kayla M. Mills, Julie A. Mahoney, Alan W. Duttlinger, Sarah K. Elefson, John S. Radcliffe, Zachary J. Rambo, Brian T. Richert Jan 2024

Effect Of Chronic Cyclic Heat Stress And Supplemented Inorganic And Organic Zinc Source Levels On Grow-Finish Pig Growth Performance And Estimated Body Composition, Kayla M. Mills, Julie A. Mahoney, Alan W. Duttlinger, Sarah K. Elefson, John S. Radcliffe, Zachary J. Rambo, Brian T. Richert

Animal and Food Sciences Faculty Publications

Zinc (Zn) supplementation has proved to mitigate the effects of heat stress with varying effects evident with Zn source during acute heat events. However, the effects of Zn supplementation during long-term summer weather patterns have yet to be explored. Therefore, the objective of this study was to identify the effects of supplementation source and level of Zn to mitigate the negative effects of long-term, cyclic heat stress in finishing swine. Six hundred cross-bred pigs were housed under thermoneutral (TN) or cyclic heat (HS) conditions simulating summer heat with acute 3-d heat waves for a 70-d study. Thermoneutral conditions were 16.7 …


Effects Of Increasing Dietary Arginine Supply During The Three First Weeks After Weaning On Pig Growth Performance, Plasma Amino Acid Concentrations, And Health Status, Jorge Y. Perez-Palencia, Christian D. Ramirez-Camba, Keith Haydon, Kristine L. Urschel, Crystal L. Levesque Jan 2024

Effects Of Increasing Dietary Arginine Supply During The Three First Weeks After Weaning On Pig Growth Performance, Plasma Amino Acid Concentrations, And Health Status, Jorge Y. Perez-Palencia, Christian D. Ramirez-Camba, Keith Haydon, Kristine L. Urschel, Crystal L. Levesque

Animal and Food Sciences Faculty Publications

A total of 425 weaned pigs (Exp. 1: 225 pigs [5.8 ± 0.9 kg]; Exp. 2: 200 pigs [6.1 ± 1.2 kg]) were used to determine the optimal dietary standardized ileal digestible (SID) arginine (Arg) level in early nursery diets based on growth and health responses. The basal diet in Exp.1 was formulated to meet SID Arg recommendation (0.66%; NRC, 2012) and in Exp. 2, SID Arg was set to simulate current industry practices for feeding nursery pigs (1.15 %). Basal diets were supplemented with 0.3%, 0.6%, 0.9%, and 1.2% of l-arginine to provide five levels of dietary SID Arg. …


Essential Oil Supplementation Improves Insulin Sensitivity And Modulates The Plasma Metabolome Of Hyperinsulinemic Horses, Caroline M.M. Loos, Shuang Zhao, Liang Li, Janet Li, Wei Han, Eric S. Vanzant, Kyle R. Mcleod Jan 2024

Essential Oil Supplementation Improves Insulin Sensitivity And Modulates The Plasma Metabolome Of Hyperinsulinemic Horses, Caroline M.M. Loos, Shuang Zhao, Liang Li, Janet Li, Wei Han, Eric S. Vanzant, Kyle R. Mcleod

Animal and Food Sciences Faculty Publications

The objective of this study was to investigate the effect of essential oil (EO) supplementation on insulin sensitivity (IS) and the plasma metabolome in insulin dysregulated (ID) horses. Horses were blocked by degree of IS and assigned randomly to treatment: oral daily bolus (50 mL) of either a plant derived EO supplement or carrier (CON). Mares were housed in dry lots with ad libitum access to grass hay and supplemented individually twice daily with a concentrate to meet nutrient requirements for mature horses. Before and after 6 wks of treatment, mares underwent a combined glucose-insulin tolerance test (CGIT) and an …


Effects Of A Second Iron-Dextran Injection Administered To Piglets During Lactation On Differential Gene Expression In Liver And Duodenum At Weaning, James L. Pierce, J. Wesley Lyons, Tyler B. Chevalier, Merlin D. Lindemann Jan 2024

Effects Of A Second Iron-Dextran Injection Administered To Piglets During Lactation On Differential Gene Expression In Liver And Duodenum At Weaning, James L. Pierce, J. Wesley Lyons, Tyler B. Chevalier, Merlin D. Lindemann

Animal and Food Sciences Faculty Publications

Six female littermate piglets were used in an experiment to evaluate the mRNA expression in tissues from piglets given one or two 1 mL injections of iron dextran (200 mg Fe/mL). All piglets in the litter were administered the first 1 mL injection < 24 h after birth. On day 7, piglets were paired by weight (mean body weight = 1.72 ± 0.13 kg) and one piglet from each pair was randomly selected as control (CON) and the other received a second injection (+Fe). At weaning on day 22, each piglet was anesthetized, and samples of liver and duodenum were taken from the anesthetized piglets and preserved until mRNA extraction. differential gene expression data were analyzed with a fold change cutoff (FC) of |1.2| P < 0.05. Pathway analysis was conducted with Z-score cutoff of P < 0.05. In the duodenum 435 genes were significantly changed with a FC ≥ |1.2| P < 0.05. In the duodenum, Claudin 1 and Claudin 2 were inversely affected by + Fe. Claudin 1 (CLDN1) plays a key role in cell-to-cell adhesion in the epithelial cell sheets and was upregulated (FC = 4.48, P = 0.0423). Claudin 2 (CLDN2) is expressed in cation leaky epithelia, especially during disease or inflammation and was downregulated (FC = -1.41, P = 0.0097). In the liver, 362 genes were expressed with a FC ≥ |1.2| P < 0.05. The gene most affected by a second dose of 200 mg Fe was hepcidin antimicrobial peptide (HAMP) with a FC of 40.8. HAMP is a liver-produced hormone that is the main circulating regulator of Fe absorption and distribution across tissues. It also controls the major flows of Fe into plasma by promoting endocytosis and degradation of ferroportin (SLC4A1). This leads to the retention of Fe in Fe-exporting cells and decreased flow of Fe into plasma. Gene expression related to metabolic pathway changes in the duodenum and liver provides evidence for the improved feed conversion and growth rates in piglets given two iron injections preweaning with contemporary pigs in a companion study. In the duodenum, there is a downregulation of gene clusters associated with gluconeogenesis (P < 0.05). Concurrently, there was a decrease in the mRNA expression of genes for enzymes required for urea production in the liver (P < 0.05). These observations suggest that there may be less need for gluconeogenesis, and possibly less urea production from deaminated amino acids. The genomic and pathway analyses provided empirical evidence linking gene expression with phenotypic observations of piglet health and growth improvements.


The 1-Back Matching/Mismatching Paradigm With Pigeons (Columba Livia): Evidence For Explicit Learning?, Daniel Peng Jan 2024

The 1-Back Matching/Mismatching Paradigm With Pigeons (Columba Livia): Evidence For Explicit Learning?, Daniel Peng

Theses and Dissertations--Psychology

With humans, a distinction can be made between explicit and implicit learning. language can be used to describe rule-based learning commonly seen in explicit, but not implicit learning. Unfortunately, we cannot do so with animals. Therefore, a non-verbal paradigm is needed to differentiate the types of learning. One method to make such a distinction is by training animals under a conditional discrimination task such as matching-to-sample with reinforcement delayed until a choice is made on the next trial: otherwise known as a 1-back procedure. In this experiment an alternating matching/mismatching to sample task under a 1-back schedule of reinforcement was …


Neural Hyperactivity During Value-Based Decision-Making In People With Daily/Near Daily Cannabis Use, Miranda Ramirez Jan 2024

Neural Hyperactivity During Value-Based Decision-Making In People With Daily/Near Daily Cannabis Use, Miranda Ramirez

Theses and Dissertations--Psychology

Value-based decision-making involves the coordinated effort of multiple brain regions to guide future choices based on past experiences. These processes are disrupted in cannabis use disorder, where individuals continue to use cannabis despite negative consequences. Reinforcement learning (RL) paradigms can be used to capture changes in the value of available options and may inform how the brain is impacted by frequent cannabis use. This study combined fMRI with behavioral modeling of probabilistic choice task data to compare value-based choices between young adults reporting daily/near daily cannabis use (CAN) and controls (CTRL). Participants selected one of two options reinforced ($0.25) at …


It Takes A Village: Impact Of Lgbtq+ Community On The Relationship Between Parenting Stress, Parent Mental Health, And Child Adjustment, Kevin Mcaweeney Jan 2024

It Takes A Village: Impact Of Lgbtq+ Community On The Relationship Between Parenting Stress, Parent Mental Health, And Child Adjustment, Kevin Mcaweeney

Theses and Dissertations--Psychology

While LGBTQ+ parented families are largely the same as their cis-het parent counterparts, they still experience unique factors exclusive to them. One such factor is access to the LGBTQ+ community. While research has emphasized the importance of community within the family system, the role the LGBTQ+ community can play for LGBTQ+ parented families is largely unexplored. Additionally, LGBTQ+ research examining parents of adolescents is similarly lacking. Utilizing a sample of LGBTQ+ parents with adolescent children, and informed by ecological systems theory, self-determination theory, and the parenting stress model, I examine the influence parent’s sense of LGBTQ+ community has on the …