Digital Commons Network^™

New Voices For A Better Society, Stephanie J. Diem, Baindu L. Bayon, Hussam Mahmoud, Mary L. Garcia- Cazarin, Michael J. Martin, Clare C. Rittschof, Patricia Silveyra, Umut A. Gurkan, Alison Boland- Reeves, Dalal Najib, Flannery Wasson

Entomology Faculty Publications

No abstract provided.

Toward Transparent Taxonomy: An Interactive Web-Tool For Evaluating Competing Taxonomic Arrangements, Oksana V. Vernygora, Felix A. H. Sperling, Julian R. Dupuis

Entomology Faculty Publications

Informative and consistent taxonomy above the species level is essential to communication about evolution, biodiversity and conservation, and yet the practice of taxonomy is considered opaque and subjective by non-taxonomist scientists and the public alike. While various proposals have tried to make the basis for the ranking and inclusiveness of taxa more transparent and objective, widespread adoption of these ideas has lagged. Here, we present TaxonomR, an interactive online decision-support tool to evaluate alternative taxonomic classifications. This tool implements an approach that quantifies the criteria commonly used in taxonomic treatments and allows the user to interactively manipulate weightings for different …

The Nonvesicular Sterol Transporter Aster-C Plays A Minor Role In Whole Body Cholesterol Balance, Ranjan Banerjee, Rachel C. Hohe, Shijie Cao, Bryan M. Jung, Anthony J. Horak, Iyappan Ramachandiran, William J. Massey, Venkateshwari Varadharajan, Natalie I. Zajczenko, Amy C. Burrows, Sumita Dutta, Maryam Goudarzi, Kala Mahen, Abigail Carter, Robert N. Helsley, Scott M. Gordon, Richard E. Morton, Christopher Strauch, Belinda Willard, Camelia Baleanu Gogonea, Valentin Gogonea, Matteo Pedrelli, Paolo Parini, J. Mark Brown

Markey Cancer Center Faculty Publications

Introduction: The Aster-C protein (encoded by the Gramd1c gene) is an endoplasmic reticulum (ER) resident protein that has been reported to transport cholesterol from the plasma membrane to the ER. Although there is a clear role for the closely-related Aster-B protein in cholesterol transport and downstream esterification in the adrenal gland, the specific role for Aster-C in cholesterol homeostasis is not well understood. Here, we have examined whole body cholesterol balance in mice globally lacking Aster-C under low or high dietary cholesterol conditions.

Method: Age-matched Gramd1c^+/+ and Gramd1c^−/− mice were fed either low (0.02%, wt/wt) or high (0.2%, …

Patient Experience Of Head And Neck Surgery With Free Flap Reconstruction., Lillian W. Dattilo, Trinity I. Russell, Chloe B. Warinner, Heather Starmer, Donald J. Annino, Laura A. Goguen, Rosh K. V. Sethi, Ravindra Uppaluri, Melina Windon, Regan W. Bergmark, Eleni M. Rettig

Markey Cancer Center Faculty Publications

IMPORTANCE: Major head and neck surgery with microvascular free tissue transfer reconstruction is complex, with considerable risk of morbidity. Little is known about patients' experiences, including decision-making prior to, and regret following, free flap surgery.

OBJECTIVE: To characterize patient experiences and decision regret of patients undergoing head and neck reconstructive free flap surgery.

DESIGN, SETTING, AND PARTICIPANTS: This mixed-methods cohort study comprising semistructured interviews was conducted June to August 2021 at a single tertiary academic cancer center. Participants underwent head and neck reconstructive surgery with microvascular free tissue transfer (flap) more than 3 months before recruitment (range, 3 months to …

Antioxidant Properties Of Bovine Liver Protein Hydrolysates And Their Practical Application In Biphasic Systems, Betul Arslan, Youling L. Xiong, Ayla Soyer

Animal and Food Sciences Faculty Publications

BACKGROUND: The influence of protein hydrolysate produced from bovine liver protein hydrolysate (LPH) by enzymatic hydrolysis, using Alcalase/Protamex (1:1), on lipid dispersions was investigated. LPH production was optimized to maximize the antioxidant activity (at 45, 50, and 55 °C for 12, 18, and 24 h). Different concentrations of LPHs (1, 3, and 5 mg/g) were added to emulsions and to liposomes. Lipid oxidation level and particle size of the lipid dispersions were monitored for 14 days of storage at 25 °C. RESULTS: Radical scavenging activity and reducing power were the highest at 45 °C after 24 h of hydrolysis. Electrophoresis …

Colorectal Cancer Survival Disparities In The Five Regions Of Georgia, Meng-Han Tsai, Daramola N. Cabral, Caitlyn Grunert, Justin X. Moore

Markey Cancer Center Faculty Publications

Background/objective

The objective of this study was to examine 5-year colorectal cancer survival rates. We also determined whether demographics, tumor characteristics, and treatment modality were associated with 5-year CRC survival in the Clayton, West Central, East Central, Southeast, and Northeast Georgia regions because the significant higher CRC mortality rates in these regions in comparison to the overall rates in the State of Georgia.

Methods

We conducted a retrospective cohort analysis using data from the 1975–2016 Surveillance, Epidemiology, and End Results program aggregated CRC patients to these five regions. Five-year CRC survival was calculated and stratified by the five regions of …

Altered Lipid Metabolism In Apc-Driven Colorectal Cancer: The Potential For Therapeutic Intervention, Courtney O. Kelson, Yekaterina Y. Zaytseva

Markey Cancer Center Faculty Publications

Altered lipid metabolism is a well-recognized feature of solid cancers, including colorectal cancer. In colorectal cancer, upregulation of lipid metabolism contributes to initiation, progression, and metastasis; thus, aberrant lipid metabolism contributes to a poor patient outcome. The inactivating mutation of APC, a vital tumor suppressor in the Wnt signaling pathway, is a key event that occurs early in the majority of colorectal cancer cases. The potential crosstalk between lipid metabolism and APC-driven colorectal cancer is poorly understood. This review collectively highlights and summarizes the limited understanding between mutations in APC and the upregulation of Wnt/beta- catenin signaling and lipid metabolism. …

Morphological And Physiological Response Of Planeleaf Willow (Salix Planifolia Pursh.) To Simulated Browsing, L Xu, J L. Dodd, M A. Smith, Q D. Skinner, W A. Laycock

IGC Proceedings (1977-2023)

Morphological and physiological responses of planeleaf willow (Salix planefolia Pursh.) to simulated browsing were studied under controlled conditions. The treatments consisted of every combination of three clipping intensities (30%, 60% and 90% of current twigs length removal) and three clipping timings (late winter, early spring and mid-summer). Increased clipping intensity stimulated bud activation; increased total leaf area and leaf size and increased the length of current year’s twigs. Mid-summer browsing increased the total number of leaves, the number of current twigs and decreased the length of current twigs. Higher browsing intensity resulted in higher photosynthetic rate of recently matured …

Interferons In Viral Infections, Pracheta Sengupta, Saurabh Chattopadhyay

Markey Cancer Center Faculty Publications

No abstract provided.

Integration Of Pan-Omics Technologies And Three-Dimensional In Vitro Tumor Models: An Approach Toward Drug Discovery And Precision Medicine., Anmi Jose, Pallavi Kulkarni, Jaya Thilakan, Murali Munisamy, Anvita Gupta Malhotra, Jitendra Singh, Ashok Kumar, Vivek M. Rangnekar, Neha Arya, Mahadev Rao

Markey Cancer Center Faculty Publications

Despite advancements in treatment protocols, cancer is one of the leading cause of deaths worldwide. Therefore, there is a need to identify newer and personalized therapeutic targets along with screening technologies to combat cancer. With the advent of pan-omics technologies, such as genomics, transcriptomics, proteomics, metabolomics, and lipidomics, the scientific community has witnessed an improved molecular and metabolomic understanding of various diseases, including cancer. In addition, three-dimensional (3-D) disease models have been efficiently utilized for understanding disease pathophysiology and as screening tools in drug discovery. An integrated approach utilizing pan-omics technologies and 3-D in vitro tumor models has led to …

Selection Of Interspecific Hybrids Of Brachiaria-A Tropical Forage Grass, C B. Do Valle

IGC Proceedings (1977-2023)

The aim of the breeding program at the National Beef Cattle Research Center (CNPGC) of the Brazilian Corporation for Agricultural Research (EMBRAPA), is to produce vigorous apomictic hybrids with wide edaphic adaptation, good production and nutritive value combined with spittlebug (Homoptera:Cercopidea) resistance. Fiftysix superior hybrids were selected based on visual estimates of vigor, leafiness, tolerance to drought and natural spittlebug infestation in the field. These were characterized for mode of reproduction, spittlebug reaction under controlled conditions and for morphological diversity using nine selected traits. Multivariate analysis classified the population into seven groups where the hybrids were clearly intermediate in plant …

Western Science And Eastern Zen To Seek The Origin Of Truth: Philosophical Background Of Scale Modeling, Kozo Saito

Progress in Scale Modeling, an International Journal

This article was written to introduce philosophical background of scale modeling, where Zen philosophy was applied to overcome the limitation of logical thinking and hypotheses-driven deductive science. Three specific reasons are as follows. The first is related to the law approach in scale modeling; it uses the kufu principle, originated in Zen Buddhism, together with the other three scientific methods: experimental, theoretical, and computational. The second reason is because scale modeling seeks relativistic understanding by attempting to realize similarity; the concept is closer to Eastern philosophy rather than absolute understanding cultivated by deductive science. The third is in the educational …

Forage News [2024-03], University Of Kentucky

Forage News

No abstract provided.

Wastewater Surveillance For Identifying Sars-Cov-2 Infections In Long-Term Care Facilities, Kentucky, Usa, 2021–2022, James W. Keck, Reuben Adatorwovor, Matthew Liversedge, Blazan Mijotavich, Cullen Olsson, William D. Strike, Atena Amirsoleimani, Ann Noble, Soroosh Torabi, Alexus Lori Rockward, Mohammad Dehghan Banadaki, Ted Smith, Parker Lacy, Scott M. Berry

UK CARES Faculty Publications

Persons living in long-term care facilities (LTCFs) were disproportionately affected by COVID-19. We used wastewater surveillance to detect SARS-CoV-2 infection in this setting by collecting and testing 24-hour compos- ite wastewater samples 2–4 times weekly at 6 LTCFs in Kentucky, USA, during March 2021–February 2022. The LTCFs routinely tested staff and symptomatic and exposed residents for SARS-CoV-2 using rapid antigen tests. Of 780 wastewater samples analyzed, 22% (n = 173) had detectable SARS-CoV-2 RNA. The LTCFs reported 161 positive (of 16,905) SARS-CoV-2 clinical tests. The wastewater SARS-CoV-2 signal showed vari- able correlation with clinical test data; we observed the strongest …

Online Instructional Tools For Motor Carriers, Brian Howell, Jennifer Walton, Paul Ross, Andrew Martin

Kentucky Transportation Center Research Report

Commercial motor carrier customers on Kentucky highways must register and pay taxes for licensing and credentialing requirements. The Kentucky Transportation Cabinet (KYTC) provides an online Motor Carrier Portal that offers information and instructions on meeting these requirements, but customers have increasingly resorted to calling into KYTC’s motor carrier help desk, thereby straining limited personnel resources. The Kentucky Transportation Center (KTC) coordinated with KYTC to develop a series of online instructional videos that can better assist customers with applying for and meeting credentialing requirements.

KTC researchers used a three-phase approach to determine the feasibility and requirements for instructional videos. First, they …

Impact Of The New Context Functional Classifications For Kytc, Arlen Sandlin, Jill Asher, Nikiforos Stamatiadis, Jeff Jasper, Rachel Catchings, Chris Van Dyke

Kentucky Transportation Center Research Report

State transportation agencies are adopting an expanded context classification system to inform project development and delivery. This system classifies roadways into one of five categories based on factors such as level of development, building densities and setbacks, multimodal user patterns and requirements, network permeability, and speed. Compared to functional classification, context classification better captures the types of mobility, travel patterns, and user mixes observed in specific contexts. The expanded context classification system is found in AASHTO’s A Policy on Geometric Design of Highways and Streets (7^th Edition). The forthcoming 8^th edition will deepen integration of context classification throughout …

Chinese Digital Platform Companies’ Expansion In The Belt And Road Countries, Yujia He

Patterson School of Diplomacy and International Commerce Faculty Publications

The emergence of digital platforms is shifting the digital economy toward a platform economy, and Chinese platform-based businesses like Alibaba, Tencent, and JD are increasingly expanding in the Global South. Alongside this, the Chinese government has been promoting digital economy collaboration with emerging markets through high-level engagement under the banner of the Belt and Road Initiative (BRI) and its digital economy component the Digital Silk Road (DSR). Despite significant market interest and policy attention, grounded empirical analysis of Chinese digital platforms’ expansion within Belt and Road Initiative countries is scarce. This study employs a mixed-methods approach, drawing on both quantitative …

Adverse Childhood Experiences And Early Initiation Of Substance Use: A Survival Analysis., Amy L. Meadows, Justin C Strickland, S Maela Hyder, Rita C Basconi, Margaret E Stull, Frances P Wagner, Mai N Nguyen, Abner O. Rayapati, Craig R Rush

Psychiatry Faculty Publications

OBJECTIVE: Early adversity, such as adverse childhood experiences (ACEs), is a risk factor for the development of substance use disorder (SUD). ACEs are associated with earlier initiation of substance use. This study examined the relationship between ACEs and age of initiation of substance use using survival analysis. It is hypothesized that individuals with higher ACEs will have an earlier age of initiation.

METHOD: Participants were recruited from the University of Kentucky's Laboratory for Human Behavioral Pharmacology. Participants were 18 years or older, English speaking, and actively engaged in substance use. Participants were not in substance abuse treatment nor were they …

Strengthening The Deployment Of Uncrewed Aerial Systems (Uas) At Kytc, Rachel Catchings, Suzanne Smith, Gayle Marks, Candice Wallace, Christopher Van Dyke

Kentucky Transportation Center Research Report

The Kentucky Transportation Cabinet (KYTC) and transportation agencies around the United States increasingly depends on uncrewed aircraft systems (UAS — also commonly known as drones) to collect data and accelerate project development and delivery. Drones let transportation practitioners safely and efficiently perform many activities, including bridge and highway inspection, construction monitoring, surveying, emergency response, and measuring stockpiles. KYTC’s implementation of UAS has expanded rapidly over the past 10 years, however, the agency lacks well-coordinated procedures for connecting pilots with end data users, scheduling and conducting flights, developing and documenting best practices, and processing and storing data. Based on a review …

Best Practices For Recruitment And Retention Of Transportation Professionals, Chris Van Dyke, Bryan Gibson, Sarah Mccormack, Merl Hackbart, Doug Kreis

Kentucky Transportation Center Research Report

Many public sector agencies, including state departments of transportation (DOTs), have long found recruiting and retaining staff a daunting challenge. Lured by the promise of higher salaries in the private sector, many people are reluctant to sacrifice their long-term earnings potential to pursue public service. And with the quality of fringe benefits (e.g., retirement plans, health insurance) offered by public agencies having declined gradually, it is even more difficult to persuade prospective employees to accept a lower salary. Many studies over the past 15—20 years have looked at methods for solving the problem of recruitment and retention within the context …

Of Hearth And Home: Professional Wellness After Hours, Timothy J. Ainger, Meriem Bensalem-Owen, Joanna Fong-Isariyawongse, Matthew William Luedke

Neurology Faculty Publications

Burnout is a prevalent problem in the contemporary practice of medicine. Defined by the Agency for Healthcare Research and Quality as, “a long-term stress reaction marked by emotional exhaustion, depersonalization, and a lack of sense of personal accomplishment,” this multifactorial condition has significant implications for the clinicians who suffer it, their patients, and families. Neurologists suffer some of the highest rates of burnout. Burnout research on interventions often focus on the work environment. In this article, we will focus on burnout’s effects on home life and features of home life that can impact resiliency, specifically sleep hygiene.

Mnemonic Utilization In Stroke Education: Fast And Befast Adoption By Certified Comprehensive Stroke Centers, Christopher Hogge, Larry B. Goldstein, Sushanth R. Aroor

Neurology Faculty Publications

Introduction: Symptom recognition and timely access to treatment are critical components of acute stroke care systems. Two mnemonics widely used in public educational campaigns for recognizing stroke symptoms include FAST (Face-Arm-Speech-Time) and BEFAST (Balance-Eyes-Face-Arm Speech-Time). The FAST mnemonic can miss up to 14% of strokes. BEFAST includes common posterior circulation stroke symptoms and has been implemented by several Comprehensive Stroke Centers (CSCs).

Methods: We sought to analyze the pattern of public educational materials available on the websites of US CSCs. The Joint Commission (JC) quality check website compiles a list containing the names and locations of the country’s 217 JC-certified …

Equity Of Overdose Education And Naloxone Distribution Provided In The Kentucky Healing Communities Study, Douglas R. Oyler, Hannah K. Knudsen, Carrie B. Oser, Sharon L. Walsh, Monica Roberts, Shawn R. Nigam, Philip M. Westgate, Patricia R. Freeman

Biostatistics Faculty Publications

Background: Opioid overdoses differentially affect demographic groups. Strategies to reduce overdose deaths, specifically overdose education and naloxone distribution (OEND), are not consistently delivered equitably. Methods: The HEALing Communities Study^SM (HCS) is a cluster-randomized trial designed to implement evidence-based practices, including OEND, to reduce overdose deaths across communities. Individuals receiving OEND in eight Kentucky counties between January 2020 and June 2022 provided demographics and overdose history. Recipient characteristics were compared to opioid overdose decedent characteristics to evaluate whether OEND was equitably delivered to the target population. Recipient characteristics were also analyzed based on whether OEND was delivered in criminal justice, …

Older Adults Using Social Support To Improve Self-Care (Oasis), Brittany L. Smalls, Aaron Kruse-Diehr, Courtney L. Ortz, Key Douthitt, Christopher Mclouth, Rachel Shelton, Zoe Taylor, Edith Williams

Biostatistics Faculty Publications

Introduction The prevalence of type 2 diabetes (T2D) is 17% higher in rural dwellers compared to their urban counterparts, and it increases with age, with an estimated 25% of older adults (≥ 65 years) diagnosed. Appropriate self-care is necessary for optimal clinical outcomes. Overall, T2D self-care is consistently poor among the general population but is even worse in rural-dwellers and older adults. In rural Kentucky, up to 23% of adults in Appalachian communities have been diagnosed with T2D and, of those, 26.8% are older adults. To attain optimal clinical outcomes, social environmental factors, including social support, are vital when promoting …

Spontaneous Alignment Of Myotubes Through Myogenic Progenitor Cell Migration, Lauren E. Mehanna, Adrianna R. Osborne, Charlotte A. Peterson, Brad J. Berron

Chemical and Materials Engineering Faculty Publications

In large-volume muscle injuries, widespread damage to muscle fibers and the surrounding connective tissue prevents myogenic progenitor cells (MPCs) from initiating repair. There is a clinical need to rapidly fabricate large muscle tissue constructs for integration at the site of large volume muscle injuries. Most strategies for myotube alignment require microfabricated structures or prolonged orientation times. We utilize the MPC’s natural propensity to close gaps across an injury site to guide alignment on collagen I. When MPCs are exposed to an open boundary free of cells, they migrate unidirectionally into the cell-free region and align perpendicular to the original boundary …

Abdominal Aortic Aneurysms And Platelets: Infiltration, Inflammation, And Elastin Disintegration, Bowen Li, Hong S. Lu, Alan Daugherty

Saha Cardiovascular Research Center Faculty Publications

No abstract provided.

Financial-Legal Navigation Reduces Financial Toxicity Of Pediatric, Adolescent, And Young Adult Cancers, Jean Edward, Kimberly D. Northrip, Mary Kay Rayens, Andrea Welker, Rachel O'Farrel, Jennifer Knuf, Haafsah Fariduddin, Julia Costich, John A. D'Orazio

Markey Cancer Center Faculty Publications

Background: Pediatric, adolescent, and young adult patients with cancer and their caregivers are at high risk of financial toxicity, and few evidence-based oncology financial and legal navigation programs exist to address it. We tested the feasibility, acceptability, and preliminary effectiveness of Financial and Insurance Navigation Assistance, a novel interdisciplinary financial and legal naviga- tion intervention for pediatric, adolescent and young adult patients and their caregivers.

Methods: We used a single-arm feasibility and acceptability trial design in a pediatric hematology and oncology clinic and collected preintervention and postintervention surveys to assess changes in financial toxicity (3 domains: psychological response/ Comprehensive Score …

Staphylococcus Aureus Oleate Hydratase Produces Ligands That Activate Host Ppara, Christopher D. Radka, Matthew W. Frank, Tyler S. Simmons, Cydney N. Johnson, Jason W. Rosch, Charles O. Rock

Markey Cancer Center Faculty Publications

Commensal gut bacteria use oleate hydratase to release a spectrum of hydroxylated fatty acids using host-derived unsaturated fatty acids. These compounds are thought to attenuate the immune response, but the underlying signaling mechanism(s) remain to be established. The pathogen Staphylococcus aureus also expresses an oleate hydratase and 10- hydroxyoctadecanoic acid (h18:0) is the most abundant oleate hydratase metabolite found at Staphylococcal skin infection sites. Here, we show h18:0 stimulates the transcription of a set of lipid metabolism genes associated with the activation of peroxisome proliferator activated receptor (PPAR) in the RAW 264.7 macrophage cell line and mouse primary bone marrow-derived …

Associations Between Personality And Mental Health Among Royal Canadian Mounted Police Cadets, Katie L. Andrews, Laleh Jamshidi, Jolan Nisbet, Tracie O. Afifi, Shannon Sauer-Zavala, Gregory P. Kratzig, Taylor A. Teckchandani, J. Patrick Neary, R. Nicholas Carleton

Psychology Faculty Publications

Royal Canadian Mounted Police (RCMP) report frequent exposures to diverse potentially psychological traumatic events (PPTEs) that can lead to symptoms of posttraumatic stress disorder (PTSD) and other mental health disorders. Personality traits may partially inform the substantial mental health challenges reported by serving RCMP. The current study examines associations between HEXACO personality factor and facet-level dimensions and mental health disorders of RCMP cadets starting the Cadet Training Program (CTP). RCMP cadets (n = 772) starting the CTP self-reported sociodemographics, personality, and mental health disorder symptoms. Emotionality was associated with MDD, GAD, and SAD (AORs ranged from 6.23 to 10.22). Extraversion …

Eeg-Meta-Microstates, Thomas Koenig, Sarah Diezig, Sahana Nagabhushan Kalburgi, Elena Antonova, Fiorenzo Artoni, Lucie Brechet, Juliane Britz, Pierpaolo Croce, Anna Custo, Alena Damborská, Camila Deolindo, Markus Heinrichs, Tobias Kleinert, Zhen Liang, Michael M. Murphy, Kyle Nash, Chrystopher Nehaniv, Bastian Schiller, Una Smailovic, Povilas Tarailis, Miralena Tomescu, Eren Toplutaş, Federica Vellante, Anthony Zanesco, Filippo Zappasodi, Qihong Zou, Christoph M. Michel

Psychology Faculty Publications

Over the last decade, EEG resting-state microstate analysis has evolved from a niche existence to a widely used and well-accepted methodology. The rapidly increasing body of empirical findings started to yield overarching patterns of associations of biological and psychological states and traits with specific microstate classes. However, currently, this cross-referencing among apparently similar microstate classes of different studies is typically done by “eyeballing” of printed template maps by the individual authors, lacking a systematic procedure. To improve the reliability and validity of future findings, we present a tool to systematically collect the actual data of template maps from as many …