Digital Commons Network^™

Caspase-11 And Nlrp3 Exacerbate Systemic Klebsiella Infection Through Reducing Mitochondrial Ros Production, Yuqi Zhou, Zhuodong Chai, Ankit Pandeya, Ling Yang, Yan Zhang, Guoying Zhang, Congqing Wu, Zhenyu Li, Yinan Wei

Chemistry Faculty Publications

Introduction: Klebsiella pneumoniae is a Gram-negative bacterium and the third most commonly isolated microorganism in blood cultures from septic patients. Despite extensive research, the mechanisms underlying K. pneumoniae-induced sepsis and its pathogenesis remain unclear. Acute respiratory failure is a leading cause of mortality in systemic K. pneumoniae infections, highlighting the need to better understand the host immune response and bacterial clearance mechanisms. Method: To investigate the impact of K. pneumoniae infection on organ function and immune response, we utilized a systemic infection model through intraperitoneal injection in mice. Bacterial loads in key organs were quantified, and lung injury was assessed. …

Iodinated Disinfection Byproduct Formation From Iohexol In Sunlit And Chlorinated Urban Wastewaters, Reagan Patton Witt, Marcelo I. Guzman

Chemistry Faculty Publications

Iodinated disinfection by-products (I-DBPs) are of growing concern due to their elevated toxicity compared to their chlorinated counterparts, with links to adverse health effects such as bladder cancer and miscarriages. Medical imaging agents like iohexol, commonly used in healthcare facilities, introduce iodine into wastewater systems. This study investigates the photodegradation of iohexol and the subsequent formation of products, including I-DBPs, during simulated final wastewater treatment under chlorination and sunlight exposure. Experiments were conducted with solutions containing 30 μM iohexol, 3 mg L⁻¹ humic acids, and 5.5 mg L⁻¹ hypochlorite. Samples were irradiated at λ ≥ 295 nm and …

Aluminum In Beverages And Foods: A Comprehensive Compilation Of Regulations; Concentrations In Raw, Prepared, And Stored Beverages And Foods; And Intake, Robert Yokel

Pharmaceutical Sciences Faculty Publications

The history and controversy concerning aluminum use in beverage and food preparation and storage are reviewed. This review on aluminum in drinking water, foods, and beverages updates and exceeds prior reviews. Country and international organization regulations that permit incorporation of aluminum containing food additives, their use limit, and purposes are presented. The aluminum concentration in beverages and foods consumed worldwide was gleaned from approximately 2100 sources available to the author. The approximately 17700 values are compiled in 175 tables by beverage or food category that include the analytical method used, sample source, and reference. The tables are posted on the …

Effects Of Fentanyl-Adulterated Methamphetamine On Circulating Ghrelin In Rats, Huimei Wei, Elise C. Maul, Shawn Park, Kaniz Fatema, Daniel J. Peter, Chang-Guo Zhan, Fang Zheng

Pharmaceutical Sciences Faculty Publications

The appetite hormone ghrelin influences biological processes that are responsible for substance use disorder, which is related to alcohol and most abused drugs including cocaine, methamphetamine, nicotine, etc. In general, upregulation of the ghrelin system enhances drug cravings and substance use. Studies reported in the literature consistently demonstrated that the ghrelin system is associated with stimulants. However, research on opioids in combination with methamphetamine has not been reported. In this study, we examined the relationship of circulating ghrelin with the polydrug use of fentanyl and methamphetamine in male Sprague-Dawley rats, demonstrating for the first time that concurrent use of fentanyl …

Effects Of Fentanyl-Laced Cocaine On Circulating Ghrelin, Insulin, And Glucose Levels In Rats, Huimei Wei, Elise C. Maul, Annet Kyomuhangi, Shawn Park, Maddilynn L. Mutchler, Chang-Guo Zhan, Fang Zheng

Pharmaceutical Sciences Faculty Publications

Opioid mixed with cocaine has been increasingly implicated in overdose deaths, including both intentional co-use of opioid and cocaine and fentanyl-adulterated drug supply. As ghrelin plays an important role in drug reward and can also influence insulin, this study aimed to assess responses of the circulating ghrelin, insulin, and glucose levels to the combined use of fentanyl and cocaine (a polydrug) in rats by performing combined animal behavioral experiments and biochemical analysis. The experimental data consistently revealed that the fentanyl and cocaine co-use can significantly elevate both the acyl-ghrelin and desacyl-ghrelin levels and significantly decrease the insulin level without significant …

Fluorescence-Guided Resection Of Gl261 Red-Fluc And Trp-Mcherry-Fluc Mouse Glioblastoma Tumors, Louis T. Rodgers, Bryan J. Maloney, Anika M. S. Hartz, Björn Bauer

Pharmaceutical Sciences Faculty Publications

Background: Most preclinical studies on glioblastoma (GBM) fail to provide translational utility in the clinic. Fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA) improves tumor resection, disease prognosis, and, thus, patient outcomes. Given the critical role of surgery in managing recurrent GBM, it is essential to incorporate surgical elements into preclinical models to accurately reflect clinical scenarios and enhance translational success. However, existing protocols for 5-ALA-guided resection in preclinical models are limited and often lack clinical relevance.

Methods: To address this gap, we developed a novel protocol for the 5-ALA-guided resection in two mouse GBM models: TRP-mCherry-FLuc and GL261 Red-FLuc.

Results: The …

Association Between Lifetime Interpersonal Violence And Post– Covid-19 Condition Among Women In Kentucky, 2020-2022, Ayşe Güler, Heather M. Bush, Katie Schill, Nurlan Kussainov, Ann L. Coker

Biostatistics Faculty Publications

Objective: The COVID-19 pandemic increased the risk of interpersonal violence. We investigated the association between lifetime interpersonal violence experience and risk of post–COVID-19 condition (the persistence of symptoms of COVID-19 and severity of health problems associated with COVID-19 that last a few weeks, months, or years) among women with lifetime interpersonal violence experience.   Methods: Women participants aged ≥18 years in Kentucky’s Wellness, Health & You—COVID-19 study completed online quantitative surveys about the impacts of the pandemic, developing COVID-19, and symptoms of post–COVID-19 condition. We conducted cross-sectional analyses estimating rate ratios of developing COVID-19 and symptoms of post–COVID-19 condition during the …

Development And Clinical Validation Of The Sensor-E Exoskeleton For Objective Hand Function Assessment In Stroke Rehabilitation, Madison Bates

Theses and Dissertations--Biomedical Engineering

Hand function is often impaired following neurological trauma or in conditions such as stroke, limiting independence and the ability to perform activities of daily living (ADLs). Current clinical assessments rely heavily on subjective rating scales that lack the resolution to detect subtle changes in motor performance, which can limit access to rehabilitation. Recent advances in wearable technologies highlight the potential for hand-worn devices to provide objective, real-time measures of hand function. However, most systems focus solely on kinematics and rarely capture both movement and exerted force. Moreover, integration into rehabilitation frameworks and brain-computer interface (BCI) protocols remains limited. To address …

Image Processing And Machine Learning Using Lidar And 360 Camera In Underground Mines, Ana Fabiola Patricia Tejada Peralta

Theses and Dissertations--Mining Engineering

Underground mining environments present several safety challenges due to low visibility, poor lighting, and the absence of GPS coverage. These factors hinder situational awareness and delay hazard detection, particularly during post-disaster conditions. This research introduces an integrated framework that combines image processing and machine learning techniques applied to 360-degree camera and LiDAR data to enable automated object detection and remote monitoring in underground mines.

The study was divided into two main stages: 2D object detection and 3D object detection. The 2D component employed images captured with a Ricoh Theta Z1 360° camera and utilized YOLO-based deep learning models for object …

Development Of A Stable Isotope Method To Measure Protein Fractional Synthesis Rate In Equine Skeletal Muscle, Antoinette E. Foliart

Theses and Dissertations--Animal and Food Sciences

Muscle mass is regulated by the balance between muscle protein synthesis and degradation. Previous research in horses has shown that the signaling pathway that regulates protein synthesis is upregulated with increasing protein intake; however, whether this corresponds to an increase in muscle protein synthesis rates had not been previously explored. The objective of this study was to develop an isotopic method to measure muscle protein fractional synthesis rate (FSR) in horses following graded protein intake. Eight mature Thoroughbred horses were studied using a randomized cross-over design. Horses consumed meals containing 0 (No), 0.125 (Low), 0.25 (Med), or 0.5 (High) g …

Where Does The Money Go? Three Essays On Intergovernmental Transfers, Spending, And Poverty In Indonesia, Jacobus Cd Rijoly

Theses and Dissertations--Public Policy and Administration

Indonesia allocates substantial intergovernmental transfers to local governments; nonetheless, the decrease in poverty remains limited. This dissertation posits that the binding constraint is institutional, specifically the regulations and incentives that dictate the extent to which funds are allocated from the government to designated programs. Institutional pass-through refers to the fraction of a transfer that is allocated to a program expenditure. The three essays integrate estimation, budget composition evidence, and a formal model to explain both modest average effects and heterogeneity across regions and programs.

The first essay evaluates the impact of transfers on provincial poverty from 2001 to 2022 by …

Essays On Public And Nonprofit Financial Management In Times Of Crisis, Junoh Jeon

Theses and Dissertations--Public Policy and Administration

This dissertation comprises three essays that examine critical aspects of public and nonprofit financial management, focusing on interorganizational relationships, local government influence, and financial reserve management in times of crisis.

The first essay examines how interorganizational relationships, specifically nonprofit organizations’ board interlocks, relate to their financial distress, depending on the temporal and spatial contexts. Organization-level fixed-effects regression analysis with Kentucky nonprofits from 2018 to 2022 shows that nonprofits with board interlocks in urban areas were less likely to experience financial distress during the COVID-19 pandemic. I suggest that board interlock acts as an adaptive buffering tool that helps manage resource …

Daily Anxiety And Dual Use Of Cigarettes And Vaping Products: Disparities By Sex, Race, And Ethnicity In U.S. Adults, Maggie K. Richardson, Delvon T. Mattingly, Osayande Agbonlahor, Alison C. Mcleish, Joy L. Hart, Kandi L. Walker

Educational, School, and Counseling Psychology Faculty Publications

Objective:

Despite a decline in cigarette use, the dual use of cigarettes and electronic vaping products (EVPs) is a concerning pattern of nicotine consumption in the United States. Anxiety, a risk factor for tobacco use, may contribute to dual-use patterns; however, the association between daily anxiety symptoms and dual use of cigarettes and EVPs is not known. This study investigated associations between daily anxiety symptoms and dual cigarette and EVP use overall and by sex, race, and ethnicity.

Method:

Data are from the 2020–2022 National Health Interview Survey (n = 83,544). Multinomial logistic regression models, adjusted for sociodemographic factors, …

Sonic Photovoice And Critical Literacy: Listening To And Sounding What Is Me, What Is Not Me, And What Is Between, Alexander Chadwell

Theses and Dissertations--Curriculum and Instruction

This study examined sonic photovoice, a sound-based arts research method, as a tool for developing critical literacy in middle and high school students. Participants found and recorded sounds related to three categories, “what is me,” “what is not me,” and “what is between,” composed a sound collage using the sounds, and reflected on the experience. Framed by 4EA cognition, participatory sensemaking, and relational and everyday aesthetics, the study explored how listening and sounding shaped students’ conceptions of self and reality. Data from recordings, journals, and interviews suggest that the sonic engagement can foster relational, emergent understandings of self and …

Examining The Relationship Between Cognitive Auditory Processing And Sex Hormones, Trey A. Cline

Theses and Dissertations--Gerontology

Estrogen and progesterone have demonstrated modulatory effects on central auditory nervous system (CANS) function. Post-menopausal females often present to audiology clinics with complaints of difficulty hearing in background noise. However, these individuals often have normal peripheral hearing sensitivity on standard audiometry. Previous studies using the auditory brainstem response (ABR) in post-menopausal females demonstrated longer response latencies compared to their premenopausal counterparts, suggesting that sound does not transmit through the CANS as efficiently. However, to date, the available literature focused on sex hormones and the CANS relies mainly on objective measures, predominantly the ABR. The ABR reflects physiologic function through the …

High-Resolution Geospatial Database: National Criteria-Air-Pollutant Concentrations In The Contiguous U.S., 2016–2020, Tianjun Lu, Sun-Young Kim, Julian D. Marshall

Epidemiology and Environmental Health Faculty Publications

Concentration estimates for ambient air pollution are used widely in fields such as environmental epidemiology, health impact assessment, urban planning, environmental equity and sustainability. This study builds on previous efforts by developing an updated high-resolution geospatial database of population-weighted annual-average concentrations for six criteria air pollutants (PM_2.5, PM₁₀, CO, NO₂, SO₂, O₃) across the contiguous U.S. during a five-year period (2016–2020). We developed Land Use Regression (LUR) models within a partial-least-squares–universal kriging framework by incorporating several land use, geospatial and satellite–based predictor variables. The LUR models were validated using conventional …

Evaluating Impact Of Stress First Aid Programs In A Primary Care Setting, Ryan Marler

DNP Projects

Background: Prolonged stress in healthcare workers (HCWs) can lead to burnout and absenteeism. High stress also negatively impacts work productivity and healthcare quality. The Stress First Aid (SFA) program aims to improve recovery from stress among HCWs. Chronic stress is evident in primary care clinics, where absenteeism and burnout symptoms have increased.

Purpose: This project aimed to evaluate the SFA program's effectiveness in reducing burnout and absenteeism and improving healthcare workers (HCW) knowledge of stress and available resources at University of Kentucky Healthcare (UKHC).

Methods: A quasi-experimental quality improvement project included pre- and post-education surveys, an SFA education session, and …

Regulation Of Human Dna Mismatch Repair, Breanna Knicely

Theses and Dissertations--Toxicology and Cancer Biology

The DNA mismatch repair (MMR) pathway identifies and corrects misincorporations that arise during DNA replication. MMR is a pathway that is critical for maintaining genomic stability. Mutations within the MMR pathway, both germline and somatic, lead to the development of cancer. There are a variety of mechanisms that regulate the MMR pathway including protein-protein interactions, DNA damage response, and post-translational modifications. In this study, we aim to dive deeper into each of these mechanisms to obtain novel information regarding the mechanisms and regulation of human DNA MMR. In this study we generated hExo1 constructs mutating Exo1’s interactions with MSH2 and …

The Paradox Of Survival: Gendered Food Insecurity, Labor Inequalities, And Coping Strategies During The 2023/4 Drought In Eastern Ethiopia, Meron E. Lemma

Theses and Dissertations--Geography

In drought-prone regions of Eastern Ethiopia, recurring droughts profoundly reshape everyday life in ways that deepen existing gendered inequalities. Drawing on fieldwork conducted in Fedis district, including semi-structured interviews with women, project implementers, and government officials, as well as group discussions with community members, this study explores how women experience, navigate, and resist the gendered impacts of drought. By attending to the sociopolitical dynamics of drought and the development interventions that accompany it, I analyze how these forces restructure labor, land, food systems, and authority in ways that reproduce and exacerbate existing inequalities. Women’s coping strategies, ranging from begging and …

Commercial Attention Spaces: A Century Of Discourses, Measurements, And Models Of Attention In The Construction Of U.S. Advertising Markets, Jacob Saindon

Theses and Dissertations--Geography

A vast assemblage of networked devices populate our everyday spaces. Concomitant with the increasing ubiquity of media devices are advertisements. Indeed, many networked devices, specifically the “free-to-use” software applications, websites, or platforms displayed through them, are advertising-dependent. An assortment of firms within the commercial media industry—brands, agencies, publishers, vendors—work in concert to sustain a market for advertising. The core operation of the commercial media industry, I argue, is the production and exchange of attention as an economic object. In this study, I approach the production of attention and the framing of its markets as spatial processes. The continued growth of …

A Geopolitical Economy Analysis Of China And India’S Approaches To Transnational Data Governance, Yujia He, Ka Zeng

Patterson School of Diplomacy and International Commerce Faculty Publications

Recent literature on the behavior of rising powers in digital trade and data governance highlights their discourses of data sovereignty and desire to preserve domestic policy autonomy. This article contributes to the literature by employing a political economy lens that shifts the focus from the nation-state/inter-state framework towards the dynamics of state–capital relations, allowing for a more historical and contextual understanding of the geopolitics of data governance in emerging economies. Using China and India—two of the largest emerging economies—as comparative cases, and drawing on secondary data from government documents and other sources, the article argues that the interplay between the …

Igc Oral Presentation Summary: Xxv Igc Congress, S. Ray Smith, Echo Elizabeth Gotsick, J. R. Weinert-Nelson, Alayna Jacobs, Christopher D. Teutsch, Will Fleming, D. R. Woodfield, James L. Klotz, Jeff Lehmkuhler, Greg S. Halich, Brittany Davis, Brittany Hendrix, Krista Lea

IGC Proceedings (1977-2023)

This paper will give an overview of the XXV International Grassland Congress thematic, keynote, and plenary sessions. The theme of the XXV IGC Congress meeting was “Grasslands for Soil, Animal, and Human Health.” We had many people attending, presenting, and participating in all these sessions. Within a month after the conclusion of the Congress, the proceeding’s papers presented at the meeting will be available on the IGC app. By the end of 2023 all papers will be available for download through any search engine at internationalgrasslands.org.

“Single Girl, Married Girl:” Female Perspectives On Marriage, Maidenhood, And Motherhood In English-Language Folk Music, Avery Schanbacher

Libraries Award for Undergraduate Scholarship

In 1928, the Carter Family released “Single Girl, Married Girl.” The recording featured only Sara and Maybelle Carter, and in three short stanzas, highlighted the vastly differing lives of the single girl, who dressed fine and went where she pleased, and the married girl, with a baby on her knee, who rocked the cradle and cried. Sara and Maybelle Carter weren’t the only people who knew this song – before they recorded it for Ralph Peer at the famous Bristol sessions, it was alive as a part of musical traditions not just in their home in Scott County, Virginia, but …

Ioto: Never Open A Stiff Lid Again, Gabriel Portugal

Libraries Award for Undergraduate Scholarship

Background: From baby food to pickles, food jars are a common find in American homes. Unfortunately,  the task of opening a stiff jar lid can be very difficult for consumers with lower grip strength, parkinsons,  or arthritis. While there are a variety of products on the market to help make this easier, there exist few jar  openers that can be used by these consumers. Objective: This project aimed to research and develop  (R&D) an improved hands-free jar opener that is accessible for consumers with the most advanced  cases of limited arm strength. Methods: R&D followed a Design Thinking approach with …

Body Mass Index, Sleep, And Food Insecurity Predict Prevalence Of Anxiety And Depression In College Students, Makenzie Barr-Porter, Amelia Sullivan, Jade Mcnamara

Dietetics and Human Nutrition Faculty Publications

This study aimed to assess the relationship between mental health, health behaviors, and food security in young adults in a university environment. Young adults (N = 536) at two United States universities completed a survey assessing health behaviors and characteristics. Logistic regression determined the odds of anxiety and/or depression based on gender identity, body mass index (BMI), food security status (FSS), and sleep restfulness. Over one-third of students were food insecure (36.7%), and almost all reported anxiety (57%) and/or depression (39.1%). Students who identified as female, reported very low FSS, poor sleep, and had higher BMI were at greater odds …

Eco-Friendly Synthesis And Stability Analysis Of Cspbbr3 And Poly(Methyl Methacrylate)-Cspbbr3 Films, You-Lin Huang, Wei Li, Fuqian Yang

Mechanical Engineering Faculty Publications

This study presents an eco-friendly mechanochemical synthesis of cesium lead bromide (CsPbBr₃), eliminating the need of organic solvents and high temperatures. The synthesized CsPbBr₃ powder is used to fabricate poly(methyl methacrylate) (PMMA)-CsPbBr₃ films and CsPbBr₃ nanocrystals (NCs). The photoluminescence (PL) peaks of the emission light are centered at 541 nm, 538 nm, and 514 nm for the CsPbBr₃ powder, PMMA-CsPbBr₃ films, and CsPbBr₃ NCs, respectively, correlating with crystal sizes of 0.96, 0.56, and 0.12 μm, respectively. The PL lifetime analysis reveals decay times () of (4.18, 20.08), (5.7, 46.99), and (5.81, …

A Program Evaluation Case Study, Madelyne G. Culbertson

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This case study presents a program evaluation of a physical activity intervention implemented in Bourbon County, Kentucky, guided by the Centers for Disease Control and Prevention’s Framework for Program Evaluation in Public Health.

A Case Study In Program Evaluation, Alexis Strottman

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This paper presents a comprehensive evaluation plan for the Bourbon County Physical Activity Improvement Program, as prompted in the University of Kentucky's 2025 MPH-Health Management and Policy Capstone Case Study. The case study outlines a program developed in response to high obesity and inactivity rates in Bourbon County, Kentucky. Utilizing the CDC's 6-step program evaluation framework, the plan outlines engagement of a diverse group of stakeholders to assess the program's effectiveness in increasing physical activity among residents. The evaluation focuses on both process and outcome measures, examining the implementation of exercise facilities, community events, and an app designed to track …

A Case Study In Program Evaluation: Evaluation Of A Program Designed To Increase Activity Levels In Bourbon County Residents, Brooke M. Woodburn

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This paper provides recommendations to eliminate possible barriers in a program developed to reduce inactive levels across Bourbon County.

Increasing Hpv Vaccination Adherence In Floyd County, Ky: Give Teens Vaccines, Taylor M. Stumbo

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Cervical cancer remains a pressing public health issue, particularly in underserved and rural areas where access to preventive care is limited. This capstone addresses the urgent need to increase human papillomavirus (HPV) vaccination adherence in Floyd County, Kentucky—part of the central Appalachian region that experiences disproportionately high cervical cancer incidence and mortality. Despite the proven effectiveness of HPV vaccines in preventing the virus strains most responsible for cervical cancer, uptake in this region remains below national targets due to socioeconomic barriers, limited healthcare access, and lack of awareness. Give Teens Vaccines is a two component intervention leveraging existing healthcare systems …