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Coquia: An Operation For Forage Production, Manual Anaya-Garduno

IGC Proceedings (1977-2023)

The objective of this study was to show the potential of Coquia (Kochia scoparia L. Schrad var. esmeralda) as a good ooption for forage production in arid and semiarid zones of Mexico. This plant is drought resistant and it grows well in eroded soils. It can be a good complement in the ration of different animal species (bovines, ovines, caprines, rabbits and equines).

Rhizobial And Nutritional Responses Of Desmanthus Clay Soils Of Queensland, N J. Brandon, R A. Date, R M. Jones, R L. Clem, G A. Bahnisch, P R. Spies, A Becerra, B A. Robertson, T.W G. Graham

IGC Proceedings (1977-2023)

The objective of these studies was to determine the reasons for chlorosis of desmanthus observed at some sites in southeast Queensland. Inoculation with Rhizobium increased growth of desmanthus in 2 of 4 soils in the field and 4 of 8 soils in pots. Greatest responses occurred in soils with few or no effective native Rhizobium. Most soils however, contained native strains that were effective. Omission of sulphur significantly reduced dry weight of desmanthus grown in pots in 5 of 7 soils by 30-51% relative to plants supplied with all nutrients. Omission of P and Mo resulted in similar yield reductions …

Phosphorus Requirements In Legumes: Calibration Of A Rapid And Simple Tissue Test, A Moron

IGC Proceedings (1977-2023)

The objective of this research was to adjust and calibrate a phosphorus (P) tissue test to diagnose in a simple and rapid manner the nutritional status of commonly used legumes in Uruguay. Field experiments conducted during 1993-94 with four legumes, showed a significant yield response to increasing P fertilizer rates. A colorimetric sap analysis was used to determine P status in legumes. A strong relationship was found between P sap analysis and relative yields. A critical level of 90-100 μg P/ml in sap to attain 90% of the maximum yield was obtained.

Evaluation Of Different Nitrogen Sources On The Production And Chemical Properties Of C4-Sub-Tropic Pastures, J F. Reckling, G.H J. Kruger

IGC Proceedings (1977-2023)

Digitaria eriantha fertilised with limestone ammonium nitrate (LAN 28 % N), although not statistically significant, resulted in higher dry matter production in comparison to those plants fertilised with urea (U 46% N). Significant lower (P<0.05) soil calcium levels were found in plots fertilised with LAN. Plants fertilised with urea had double the amount of Aspartic and Glutamic acid, indicating that urea did not convert to NO3 -, but was taken up as ammonium.

Effect Of Nitrogen And Potash Fertilization On Dry Matter Yield And Mineral Composition Of Pangolagrass, J B. Andrade, C L. Justo, J.L. V. Coutinho, R M. Peres, E Ferrari Junior, V T. Paulino, W Henrique, J C. Werner, H B. Mattos

IGC Proceedings (1977-2023)

The effect of nitrogen and potash fertilizations on dry matter and protein yields and chemical composition of pangolagrass (D. decumbens Stent.) was studied in a randomized block design with four replications using a 2 x 2 factorial arrangement. Nitrogen and potash were applied after each cut as an equivalent to 3 and 2% of dry matter (65°C) yield, respectively. There were great increases in dry matter and protein yields when nitrogen alone or combined with potash was used. Potash fertilization alone had no effect on the variables described above. Nitrogen fertilization improved forage pangolagrass composition, being particularly effective on crude …

Effectivity Of Arbuscular Mycorrhizas In Sustainable Grassland Agriculture Following Conversion From Intensive Management, W R. Eason, J Scullion, E P. Scott

IGC Proceedings (1977-2023)

With increased interest in sustainable agriculture, attention has focused on the role of arbuscular mycorrhiza (AM) in the productivity of agricultural systems. AM spores were taken from grassland and grass-arable systems with either a history of high-input, conventional, or low-input, organic management, and used as a source of inoculum in host plants (Allium amelioprasum, Trifolium repens and Lolium perenne). Spores from organic management systems produced a greater yield response than those from conventional systems. Where various spore inoculum produced differences in host growth responses, it was observed that the infected root of these host plants also produced similar …

Reserving Pastures In Communal Grasslands By Agropastoralists Of Tanzania, M L. Kusekwa, A J. Mwilawa, J.K K. Msechu

IGC Proceedings (1977-2023)

Studies were conducted in “Maasai” and “Gogo” tribal communities to gain understanding of traditional management of reserved pastures. Survey techniques were used and forage production and chemical composition were estimated. Household heads were invariably old men who had no formal education. Household size averaged 14 and 11, in Maasai and Gogo tribes respectively. Most reserved pastures were privately owned. Over 90% of pastoralists preferred individual ownership of reserved grazing. Herbage production was 2510 kg DM/ha in Maasai and 3680 kg DM/ha in Gogo locations. Pastures and low protein content (5.1-6.5% CP). Constraints to reserve grazing strategies were land and water …

Influence Of Drying Method And Temperature On Ruminal Degradable Protein Of Switchgrass, D E. Farnham, K J. Moore, J R. George

IGC Proceedings (1977-2023)

The nutritional value of herbage protein fed to ruminant livestock can be influenced greatly by the extent to which it is degraded in the rumen. This study was conducted to determine if drying method and temperature alters measurements of in situ ruminal escape protein (EP) of switchgrass herbage. Switchgrass harvested at the pre-heading stage was either air dried, freeze dried, or oven dried at 38, 49, 60, or 710 C. Samples from each of the six drying treatments were digested in situ in Dacron bags for 4, 8, 12, or 16 h. Drying treatment had a significant impact on EP …

Forage News [2024-06], University Of Kentucky

Forage News

No abstract provided.

Association Of Circulating Markers Of Microbial Translocation And Hepatic Inflammation With Liver Injury In Patients With Type 2 Diabetes, Leila Gobejishvili, Vatsalya Vatsalya, Diana V. Avila, Yana B. Feygin, Craig J. Mcclain, Sriprakash Mokshagundam, Shirish Barve

Biostatistics Faculty Publications

Background: Virtually the entire spectrum of liver disease is observed in association with type 2 diabetes mellitus (T2DM); indeed, T2DM is now the most common cause of liver disease in the U.S. We conducted a pilot study to investigate the relevance of increased microbial translocation and systemic inflammation in the development of liver injury in patients with T2DM. Methods: Patients with T2DM (n = 17) and non-diabetic controls (NDC; n = 11) aged 25–80 yrs. participated in this study. Serum levels of endotoxin, calprotectin, soluble CD14 and CD163, and several inflammatory cytokines were measured. In addition to standard liver injury …

Androgen Aggravates Aortic Aneurysms Via Suppression Of Pd-1 In Mice, Xufang Mu, Shu Liu, Zhuoran Wang, Kai Jiang, Timothy S. Mcclintock, Arnold Stromberg, Alejandro V. Tezanos, Eugene S. Lee, John A. Curci, Ming C. Gong, Zhenheng Guo

Markey Cancer Center Faculty Publications

Androgen has long been recognized for its pivotal role in the sexual dimorphism of cardiovascular diseases, including aortic aneurysms (AAs), a devastating vascular disease with a higher prevalence and fatality rate in men than in women. However, the mechanism by which androgen mediates AAs is largely unknown. Here, we found that male, not female, mice developed AAs when exposed to aldosterone and high salt (Aldo-salt). We revealed that androgen and androgen receptors (ARs) were crucial for this sexually dimorphic response to Aldo-salt. We identified programmed cell death protein 1 (PD-1), an immune checkpoint, as a key link between androgen and …

Preference Of Dhurrin-Free Sorghum By Ewes, Shelby M. Gruss, Keith D. Johnson, John Scott Radcliffe, Ronald P. Lemenager, Mitchell R. Tuinstra

Animal and Food Sciences Faculty Publications

Sorghum [Sorghum bicolor (L.) Moench] is a resilient forage crop due to its drought tolerance and adaptation to low-N environments. Sorghum produces a cyanogenic glucoside called dhurrin. The breakdown of dhurrin leads to the release of hydrogen cyanide (HCN), which can cause cyanide toxicity in livestock. Dhurrin-free sorghum lines have been developed through chemical mutagenesis by mutagenizing the gene for the first enzyme, CYP79A1, in the biosynthetic pathway. The CYP79A1 mutation was bred into sorghum lines to create a dhurrin-free experimental hybrid. Grazing preference of ewes was assessed when allocated to the dhurrin-free hybrid and three commercial hybrids. Near isogenic …

Fasting And Glucose Metabolism Differentially Impact Peripheral Inflammation In Human Type 2 Diabetes, Gabriella H. Kalantar, Shubh Saraswat, Sara Santacruz-Calvo, Fatemeh Gholamrezaeinejad, Aida Javidan, Madhur Agrawal, Rui Liu, Philip A. Kern, Xiaohua Douglas Zhang, Barbara S. Nikolajczyk

Biostatistics Faculty Publications

Cytokines produced by peripheral T-helper 1/17 cells disproportionately contribute to the inflammation (i.e., metaflammation) that fuels type 2 diabetes (T2D) pathogenesis. Shifts in the nutrient milieu could influence inflammation through changes in T-cell metabolism. We aimed to determine whether changes in glucose utilization alter cytokine profiles in T2D. Peripheral blood mononuclear cells (PBMCs), CD4+ T-cells, and CD4+CD25- T-effector (Teff) cells were isolated from age-matched humans classified by glycemic control and BMI. Cytokines secreted by CD3/CD28-stimulated PBMCs and Teff were measured in supernatants with multiplex cytokine assays and a FLEXMAP-3D. Metabolic activity of stimulated CD4+ T-cells was measured by a Seahorse …

Optimization, Characterization, And Comparison Of Two Luciferase-Expressing Mouse Glioblastoma Models, Louis Rodgers, Julia A. Schulz Pauly, Bryan J. Maloney, Anika M. S. Hartz, Bjoern Bauer

UK CARES Faculty Publications

Glioblastoma (GBM) is the most aggressive brain cancer. To model GBM in research, orthotopic brain tumor models, including syngeneic models like GL261 and genetically engineered mouse models like TRP, are used. In longitudinal studies, tumor growth and the treatment response are typically tracked with in vivo imaging, including bioluminescence imaging (BLI), which is quick, cost-effective, and easily quantifiable. However, BLI requires luciferase-tagged cells, and recent studies indicate that the luciferase gene can elicit an immune response, leading to tumor rejection and experimental variation. We sought to optimize the engraftment of two luciferase-expressing GBM models, GL261 Red-FLuc and TRP-mCherry-FLuc, showing differences …

Are “10 Grams Of Protein” Better Than “Ten Grams Of Protein”? How Digits Versus Number Words Influence Consumer Judgments, Marisabel Romero, Adam W. Craig, Milica Mormann, Anand Kumar

Marketing & Supply Chain Faculty Publications

Numerical information can be communicated using different number formats, such as digits (“5”) or number words (“five”). For example, a battery product may claim to last for “5 hours” or “five hours.” And while these two formats are used interchangeably in the marketplace, it is not clear how they influence consumer judgments and behavior. Via six experimental studies, two online ad campaigns, and one large secondary dataset analysis, we find that digits, compared to number words, positively affect consumer behavior. We refer to this phenomenon as the number format effect. We further show that the number format effect occurs because …

Forage News [2024-05], University Of Kentucky

Forage News

No abstract provided.

Regulation Of Rorα Stability Through Prmt5-Dependent Symmetric Dimethylation, Gaofeng Xiong, Brynne Obringer, Austen Jones, Elise Horton, Ren Xu

Markey Cancer Center Faculty Publications

Retinoic acid receptor-related orphan receptor alpha (RORα), a candidate tumor suppressor, is prevalently downregulated or lost in malignant breast cancer cells. However, the mechanisms of how RORα expression is regulated in breast epithelial cells remain incompletely understood. Protein arginine N-methyltransferase 5 (PRMT5), a type II methyltransferase catalyzing the symmetric methylation of the amino acid arginine in target proteins, was reported to regulate protein stability. To study whether and how PRMT5 regulates RORα, we examined the direct interaction between RORα and PRMT5 by immunoprecipitation and GST pull-down assays. The results showed that PRMT5 directly bound to RORα, and PRMT5 mainly symmetrically …

Effect Of Simulated Gastrointestinal Digestion On Antioxidant, And Anti-Inflammatory Activities Of Bioactive Peptides Generated In Sausages Fermented With Staphylococcus Simulans Qb7, Hongying Li, Hongbing Fan, Zihan Wang, Qiujin Zhu, Jianping Wu

Animal and Food Sciences Faculty Publications

Dry-fermented sausages are a good source of bioactive peptides, whose stability against gastrointestinal (GI) digestion determines their bioaccessibility. This study focused on evaluating the effect of peptide extracts from sausages fermented with Staphylococcus simulans QB7 during in vitro simulated GI digestion, including peptide profi les and antioxidant and anti-infl ammatory activities. Peptides present in sausages were degraded during digestion, with molecular weight reduced from > 12 kDa to < 1.5 kDa. Besides, the content of amino acids increased from 381.15 to 527.07 mg/g, especially tyrosine being found only after GI digestion. The anti-infl ammatory activities were increased after GI digestion, however, the changes in antioxidant activities were the opposite. A total number of 255, 252 and 386 peptide sequences were identified in undigested, peptic-digested and GI-digested samples, respectively. PeptideRanker, BIOPEP-UWM and admetSAR were used to further predict the functional properties and intestinal absorption of the identified peptide sequences from GI digestion. Finally, 18 peptides were discovered to possess either antioxidant or anti-infl ammatory capacities.

Check With The Intended Audience First! Content Validation As A Method For Inclusive Research For Primary Care Engagement In Rural Appalachia, Sydeena E. Isaacs, Jennifer Schroeder Tyson, Ashley Parks, Danielle Adams

Journal of Appalachian Health

Introduction: To date, referral practices based on social determinants of health (SDOH) among primary care providers (PCPs) and clinic staff in rural regions, including Appalachian North Carolina (NC), are not well understood.

Purpose: This study aims to develop and content validate a primary care engagement (PCE) survey to assess (1) engagement and burnout; (2) referral practices; and (3) self-efficacy and confidence in making referrals based on SDOH among PCPs and clinic staff in Appalachian NC.

Methods: Using the Social-Ecological Model as a theoretical framework, researchers developed a 37-item PCE survey. Content validation was completed by a panel of experts recruited …

Influence Of Low-Level Condensed Tannins Concentrations In Temperate Forages On Sheep Performace, F Montossi, F Liu, J Hodgson, S T. Morris, T N. Barry, D F. Risso

IGC Proceedings (1977-2023)

Results from a series of six comparative lamb grazing trials with temperate forages are summarised, and compared with results from the literature. Condensed tannin (CT) concentrations in the diet ranged from 0.12% to 0.47% on a dry matter (DM) basis. Values were generally similar for Yorkshire fog (Holcus lanatus), perennial ryegrass (Lolium perenne) and annual ryegrass (L. multiflorum), each grown with white clover (Trifolium repens), in similar circumstances. Concentrations for tall fescue (Festuca arundinacea)/white clover pastures were relatively low, but may have been artefacts. Drenching with polyethylene glycol (PEG) to bind …

Tannin Contents Of Some Indigenous Browse Plants Of Botswana, A A. Aganga, C M. Tsopito, K M. Morake

IGC Proceedings (1977-2023)

A study was carried out to evaluate the tannin content and crude protein degradation of leaves and twigs from indigenous browsable trees which provide feed for grazing livestock on natural rangelands of Botswana. These browses include the families Leguminosae (Acacias), Capparidaceae (Boscias), Combretaceae (Combretum) and Tiliaceae (Grewias). These were found to be of good nutritive value, with the leaves having a high content of crude protein (9 - 16%) and a wide range of dry matter digestibility (37 - 69.82%). The correlation between crude protein disappearance and tannin content was found to be negative (-0.582) and significant (P<0.05).

Effect Of Tannin In Animal Diet On Nitrification Rate Of Pasture Soil Under Dung Patches, J R. Crush

IGC Proceedings (1977-2023)

The rate of formation of nitrate and ammonium in the soil under dung patches was compared for dung from animals fed either ryegrass (Lolium perenne)/white clover (Trifolium repens) or ryegrass/Lotus pedunculatus forage. Tannin derived from the Lotus did not inhibit ammonification in the soil under dung but nitrate formation was strongly inhibited. The results support the theory that polyphenolics may bind to nitrite in acid soils and cause immobilisation of mineral nitrogen. Apart from their effect of reducing the proportion of dietary nitrogen excreted in urine, tannins in animal diets may make a further contribution to …

Condensed Tannins In Tropical Legumes: Concentration, Astringency And Effects On The Nutrition Of Ruminants, R Barahona, C E. Lascano, R C. Cochran, J L. Morrill, E C. Titgemeyer

IGC Proceedings (1977-2023)

A feeding trial was carried out to determine the effect of extractable condensed tannins (ECT) concentration and tannin astringency in tropical legumes on nitrogen (N) digestion by sheep. Test legumes were Desmodium ovalifolium (Do) and Flemingia macrophylla (Fm) which had similar concentrations of Extractable CT (9% DM) but tannins with different degree of astringency (Do, 0.6 and Fm, 0.3 g protein bound/g of ECT). Chopped sun-dried forage of each legume was sprayed with either water (control) or polyethylene glycol (PEG, 3.5% DM) to reduce ECT and fed to 8 sheep with ruminal and duodenal canulas arranged in a replicated 4 …

Effect Of Condensed Tannin In Lotus Corniculatus And Lotus Pedunculatus On Digestion Of Rubisco In The Rumen, W C. Mcnabb, R J. Aerts, A Brand, J S. Peters, Y L. Foo, G C. Waghorn

IGC Proceedings (1977-2023)

The in vitro precipitation of ribulose-1,5-bisphosphate carboxylase (Rubisco) by condensed tannin (CT) extracted from Lotus corniculatus and Lotus pedunculatus and the effect of these CT on the in vitro rumen degradation of Rubisco was used to compare the reactivity of these CT. The chemical structure of CT from Lotus corniculatus was homogenous with epicatechin stereochemistry and mostly procyanidin units. The CT from Lotus pedunculatus was heterogenous with mostly prodelphinidin units. The amount of CT required to precipitate all the Rubisco when total soluble leaf protein was incubated with CT from Lotus corniculatus and Lotus pedunculatus was similar. Although CT from …

Nitrogen And Fiber Digestion In Sheep Fed Fresh-Frozen And Field-Dried High And Low Tannin Sericea Lespedeza, T H. Terrill, W R. Windham, C S. Hoveland, H E. Amos, B Kouakou, S Gelaye

IGC Proceedings (1977-2023)

Field-drying reduces extractable condensed tannin (CT) of sericea lespedeza [Lespedeza cuneata (Dum-Cours) G. Don], but effects on specific bound and unbound forms of CT and the relationship to protein and fiber digestion in ruminants are unclear. Intake and digestibility of fresh-frozen and field-dried high- and low-tannin sericea were investigated in a feeding trial with sheep (Ovis spp.). Field-drying had no effect on crude protein (CP) and fiber content of sericea, but shifted CT from extractable to bound forms. Digestion coefficients for CP, neutral detergent fiber (NDF), and acid detergent fiber (ADF) were lower for high-tannin sericea than the low-tannin …

Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani

IGC Proceedings (1977-2023)

The transformation of Lotus corniculatus plants with the maize gene Sn, reorganizes the tissue specificity of condensed tannins accumulation. In particular the transformed plants show an increase of tannin content in roots and a decrease in leaves. Molecular and enzymatic analyses suggest that the transgene can functionally substitute an endogenous unknown gene not expressed in roots and induces its silencing when it is expressed. These findings could have applications for reducing tannin content in unpalatable plants and for cloning genes involved in tannin synthesis.

Differences In Chemical Composition Among Provenances Of Tree Fodder Species In A Subhumid Environment: Relation To Use As Supplements, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Studies were initiated in a subhumid Southern African environment to assess the chemical composition and nutritive value of Acacia angustissima (Mill.) Kuntze, Calliandra calothyrsus Meiss. and Leucaena species. The objective was to determine variability in crude protein (CP) concentration, acid detergent fibre (ADF), neutral detergent fibre (NDF), and soluble and insoluble proanthocyanidins from fodder samples of species, subspecies and provenances. A wide range was found in these parameters. The implications of these chemical composition factors, especially proanthocyanidins, on the use of these browse fodders in livestock feeding systems are discussed.

Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker

IGC Proceedings (1977-2023)

The correlation between protein binding capacity of proanthocyanidin-rich neutral detergent fiber (NDF) and their proanthocyanidin (PAs) content was very low (r = -0.21; n = 6). These PAs-rich NDF did not affect growth of a tannin-sensitive strain of Clostridium perfringens. A substantial amount of bound PAs (75 to 92 %) disappeared from the NDF in 24 h of fermentation in an in vitro incubation medium containing rumen microbes. Addition of a tannin-binding agent, polyethylene glycol to the incubation medium containing rumen microbes and browses made free of extractable tannins or NDF rich in bound PAs increased the gas production up …

Plant Tannins- Their Role In Forage Legume Quality, J A. Alokan, V A. Aletor

IGC Proceedings (1977-2023)

Forage legumes, especially browse plants, are important in the maintenance and survival of ruminant animals. However many browse species contain high levels of polyphenolic compounds including tannins. The effects of tannins on the quality of forages are reviewed. These include reduction in palatability and intake of feeds, growth rate, digestion of fibre fractions and nitrogen utilization by animals.

The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka

IGC Proceedings (1977-2023)

Hatchling grasshoppers (Melanoplus sanguinipes Fab.) fed one accession of field grown big trefoil (Lotus uliginosis Schkuhr) containing a tannin content of 56 mg. g-1 FW, had a significantly lower mean weight than hatchlings fed field grown Lotus species and accessions with lower tannin content. Adult female grasshoppers fed L. uliginosis foliage with 32.3 mg.g-1 FW tannin also ate more feed and had a 2-fold higher gut trypsin and chymotrypsin activity than adults fed low tannin L. corniculatus foliage (0.13 mg.g-1 FW tannin). In more controlled experiments, growth at 20o C or 30o C was used to increase tannin …