Digital Commons Network^™

Fate Of Stable Isotope Label During Predation Of 15N-Tagged Wild-Type Escherichia Coli By Protozoa, Ashley M. Barton

University of Kentucky Master's Theses

Currently, bacterial movement in karst aquifers is not well understood. Use of stable isotopes to label non-pathogenic Escherichia coli as a particulate groundwater tracer in karst systems has been examined in previous studies. Loss of the stable isotope signal is anticipated in traces greater than 500 m in length. Potential loss of ¹⁵N due to predation by protozoa was examined. Filter-sterilized water from Royal Spring in Georgetown, Kentucky, was inoculated with a mixture of either Tetrahymena pyriformis or Colpoda steinii and ¹⁵N-enriched E. coli and stored in the dark at 14°C. Samples were analyzed for their nitrogen isotope …

Influence Of Fan Operation On Fan Assessment Numeration System (Fans) Test Results, Gabriela Munhoz Morello

University of Kentucky Master's Theses

The use of velocity traverses to measure in-situ air flow rate of ventilation fans can be subject to significant errors. The Fan Assessment Numeration System (FANS) was developed by the USD-ARS Southern Poultry Research Laboratory and refined at the University of Kentucky to measure air flow of fans in-situ. The procedures for using the FANS unit to test fans in-situ are not completely standardized. This study evaluated the effect of operating fan positions relative to the FANS unit for ten 1.22 m diameter fans in two types of poultry barns, with fans placed immediately next to each other and …

Lycopene And Its Potential Nutritional Role For Patients With Heart Failure, Martha J. Biddle

University of Kentucky Doctoral Dissertations

Lycopene is a antioxidant found in natural and processed foods. The connection between antioxidants and heart disease has been explored in several observational studies^1-4, yet very few investigators have examined the impact of dietary antioxidants in patient with advanced heart disease such as heart failure (HF). A novel strategy for preventing or delaying the complications of HF related to inflammation and oxidative stress may be to increase dietary lycopene.

The purpose of this dissertation was to test the impact of dietary intervention consisting of lycopene (V8® juice) on biomarkers of inflammation and oxidative stress in patients with HF; …

The Return Of The Black Bear To Eastern Kentucky: Conflict And Tolerance Between People And Wildlife, Hannah B. Harris

University of Kentucky Doctoral Dissertations

The black bear (Ursus americanus) has returned to Kentucky and is now part of a reproducing population in the southeastern Cumberland Mountain region. The broad objective of this project was to examine the interactions between people and bears, with the ultimate goal of improving bear management in a way that addresses stakeholder concerns. Using interviews of regional stakeholders, participant observation, and media reports collected between summer 2003 and fall 2006, I investigated how the presence of black bears in Harlan and Letcher counties in Kentucky has had an impact on area residents. I complemented this information with observations …

Characterization Of G10h Promoter And Isolation Of Wrky Transcription Factors Involved In Catharanthus Terpenoid Indole Alkaloid Biosynthesis Pathway, Nitima Suttpanta

University of Kentucky Doctoral Dissertations

Catharanthus roseus produces a large array of terpenoid indole alkaloids (TIAs) that are an important source of natural or semi-synthetic anticancer drugs. Biosynthesis of TIAs is tissue-specific and induced by certain phytohormones and fungal elicitors, indicating the involvement of a complex transcriptional control network. However, the transcriptional regulation of the TIA pathway is poorly understood. This study reports the isolation and characterization of the G10H promoter and two WRKY transcription factors regulating TIA biosynthesis.

Geraniol 10-hydroxylase (G10H) controls the first committed step in the biosynthesis of terpenoid indole alkaloids (TIA). The C. roseus G10H promoter sequence was isolated by a …

Forage News [2010-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Register Now for Grazing Conference
• Forage Spokesman Contest and KFGC Award Nominations
• PFGC Celebrates 50th Anniversary
• Forage Variety Test Reports
• Feed Costs and Meat Production
• Corn Nitrogen Fertilizer Replacement Value of Alfalfa in Northern Illinois
• Comparison of Seeding Rates and Coating on Seedling Count, Root Length, Root Weight and Shoot Weight of Crimson Clover
• Effect of Macronutrient Deficiency on the Antioxidant Capacity of Lespedeza Cuneata (cv. Au Grazer) and Lotus Corniculatus (cv. Pardee)

Cell Lines Having Enhanced Cell Longevity And Protein Expression, Bruce Allen Webb, Jeremy Kroemer

Entomology Faculty Patents

It is the object of the current invention to provide methods and compositions relating to the expression of vankyrin proteins in cell lines to increase their viability, longevity and capacity for protein production. The inventors have discovered that the expression of P-ank-1 and I²-ank-3 proteins in cell culture has increased the cells' longevity and capacity for endogenous and/or heterologous target protein production. Specifically, the present invention relates to the enhanced expression of endogenous and/or heterologous target proteins/polypeptides in recombinant cells that are also expressing P-ank-1 and/or I²-ank-3 protein compared to expression host cells that are not …

Adar2-Dependent Rna Editing Of Glur2 Is Involved In Thiamine Deficiency-Induced Alteration Of Calcium Dynamics, Shuchen Lee, Guang Yang, Yue Yong, Ying Liu, Liyun Zhao, Jing Xu, Xiaomin Zhang, Yanjie Wan, Chun Feng, Zhiqin Fan, Yong Liu, Jia Luo, Zun-Ji Ke

Internal Medicine Faculty Publications

BACKGROUND: Thiamine (vitamin B1) deficiency (TD) causes mild impairment of oxidative metabolism and region-selective neuronal loss in the central nervous system (CNS). TD in animals has been used to model aging-associated neurodegeneration in the brain. The mechanisms of TD-induced neuron death are complex, and it is likely multiple mechanisms interplay and contribute to the action of TD. In this study, we demonstrated that TD significantly increased intracellular calcium concentrations [Ca2+]i in cultured cortical neurons.

RESULTS: TD drastically potentiated AMPA-triggered calcium influx and inhibited pre-mRNA editing of GluR2, a Ca2+-permeable subtype of AMPA receptors. The Ca2+ permeability of GluR2 is regulated …

T-Lymphocyte Responses To Intestinally Absorbed Antigens Can Contribute To Adipose Tissue Inflammation And Glucose Intolerance During High Fat Feeding, Yuehui Wang, Jianing Li, Lihua Tang, Yu Wang, Richard Charnigo, Willem De Villiers, Erik Eckhardt

Internal Medicine Faculty Publications

BACKGROUND: Obesity is associated with inflammation of visceral adipose tissues, which increases the risk for insulin resistance. Animal models suggest that T-lymphocyte infiltration is an important early step, although it is unclear why these cells are attracted. We have recently demonstrated that dietary triglycerides, major components of high fat diets, promote intestinal absorption of a protein antigen (ovalbumin, "OVA"). The antigen was partly transported on chylomicrons, which are prominently cleared in adipose tissues. We hypothesized that intestinally absorbed gut antigens may cause T-lymphocyte associated inflammation in adipose tissue.

METHODOLOGY/PRINCIPAL FINDINGS: Triglyceride absorption promoted intestinal absorption of OVA into adipose tissue, …

Intestinal Epithelial Serum Amyloid A Modulates Bacterial Growth In Vitro And Pro-Inflammatory Responses In Mouse Experimental Colitis, Erik R.M. Eckhardt, Jassir Witta, Jian Zhong, Razvan Arsenescu, Violeta Arsenescu, Yu Wang, Sarbani Ghoshal, Marcielle C. De Beer, Frederick C. De Beer, Willem J.S. De Villiers

Internal Medicine Faculty Publications

BACKGROUND: Serum Amyloid A (SAA) is a major acute phase protein of unknown function. SAA is mostly expressed in the liver, but also in other tissues including the intestinal epithelium. SAA reportedly has anti-bacterial effects, and because inflammatory bowel diseases (IBD) result from a breakdown in homeostatic interactions between intestinal epithelia and bacteria, we hypothesized that SAA is protective during experimental colitis.

METHODS: Intestinal SAA expression was measured in mouse and human samples. Dextran sodium sulfate (DSS) colitis was induced in SAA 1/2 double knockout (DKO) mice and in wildtype controls. Anti-bacterial effects of SAA1/2 were tested in intestinal epithelial …

The Endogenous Soluble Vegf Receptor-2 Isoform Suppresses Lymph Node Metastasis In A Mouse Immunocompetent Mammary Cancer Model, Masa-Aki Shibata, Jayakrishna Ambati, Eiko Shibata, Romulo J. C. Albuquerque, Junji Morimoto, Yuko Ito, Yoshinori Otsuki

Ophthalmology and Visual Science Faculty Publications

BACKGROUND: Cancer metastasis contributes significantly to cancer mortality and is facilitated by lymphangiogenesis and angiogenesis. A new splicing variant, endogenous soluble vascular endothelial growth factor receptor-2 (esVEGFR-2) that we recently identified is an endogenous selective inhibitor of lymphangiogenesis. To evaluate the antimetastatic potential of esVEGFR-2, gene therapy with vector expressing esVEGFR-2 (pesVEGFR-2) or endostatin (pEndo) as a positive control was conducted on murine metastatic mammary cancer.

METHODS: Syngeneic inoculated metastatic mammary cancers received direct intratumoral injection of pesVEGFR-2, pEndo or pVec as control, once a week for six weeks. In vivo gene electrotransfer was performed on the tumors after each …

Forage News [2010-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Dr. Norman Taylor 1926-2010
• Registration Now Open for Grazing Conference
• Forage Spokesman Contest and KFGC Award Nominations - Deadline Dec. 3
• Effects of Ergot-Endophyte-Infected Fescue Seed on Physiological Parameters in Mature Female Meat Goats
• Chaparral® Herbicide Application for Suppression of Seedhead Emergence in Tall Fescue Pastures and Possible Alleviation of Fescue Toxicosis
• Science Shows Beef Production is Earth Friendly
• Use of Novel Endophyte Fescue for Cows
• Evaluation of Annual Ryegrass (Lolium Multiflorum) in Two Fall Grazing Systems on Forage Quality and Stock Calf Performance in Northern Minnesota
• Using Mature Hay for Bedding: Potential for Tall Fescue Toxicity

Cyanidin-3-Glucoside Inhibits Ethanol-Induced Invasion Of Breast Cancer Cells Overexpressing Erbb2, Mei Xu, Kimberly A. Bower, Siying Wang, Jacqueline A. Frank, Gang Chen, Min Ding, Shiow Wang, Xianglin Shi, Zunji Ke, Jia Luo

Internal Medicine Faculty Publications

BACKGROUND: Ethanol is a tumor promoter. Both epidemiological and experimental studies suggest that ethanol may enhance the metastasis of breast cancer cells. We have previously demonstrated that ethanol increased the migration/invasion of breast cancer cells expressing high levels of ErbB2. Amplification of ErbB2 is found in 20-30% of breast cancer patients and is associated with poor prognosis. We sought to identify agents that can prevent or ameliorate ethanol-induced invasion of breast cancer cells. Cyanidin-3-glucoside (C3G), an anthocyanin present in many vegetables and fruits, is a potent natural antioxidant. Ethanol exposure causes the accumulation of intracellular reactive oxygen species (ROS). This …

Plant Phenolics: Extraction, Analysis And Their Antioxidant And Anticancer Properties, Jin Dai, Russell J. Mumper

Pharmaceutical Sciences Faculty Publications

Phenolics are broadly distributed in the plant kingdom and are the most abundant secondary metabolites of plants. Plant polyphenols have drawn increasing attention due to their potent antioxidant properties and their marked effects in the prevention of various oxidative stress associated diseases such as cancer. In the last few years, the identification and development of phenolic compounds or extracts from different plants has become a major area of health- and medical-related research. This review provides an updated and comprehensive overview on phenolic extraction, purification, analysis and quantification as well as their antioxidant properties. Furthermore, the anticancer effects of phenolics in-vitro …

The Kentucky Food Consumer, Timothy A. Woods

Agricultural Economics Presentations

No abstract provided.

Economic Outlook 2011: U.S. Economy And Agriculture, Craig Infanger

Agricultural Economics Presentations

No abstract provided.

The Appalachian Regional Development Act And Economic Change, James P. Ziliak

University of Kentucky Center for Poverty Research Discussion Paper Series

The Appalachian Regional Development Act of 1965 is one of the longest serving place-based regional development programs in the U.S., and is the largest in terms of geographic scope. I use county-level data from the 1960 thru 2000 Decennial Censuses to evaluate the effect of ARDA on poverty rates and real per capita incomes in Appalachia. The intent to treat parameter is identified in a difference-in-difference-in-difference framework by comparing outcomes in Appalachia to her border counties. Additional knowledge of which counties were solely eligible for highway development funds under ARDA from those counties eligible for both highway as well as …

Deficient Liver Biosynthesis Of Docosahexaenoic Acid Correlates With Cognitive Impairment In Alzheimer's Disease, Giuseppe Astarita, Kwang-Mook Jung, Nicole C. Berchtold, Vinh Q. Nguyen, Daniel L. Gillen, Elizabeth Head, Carl W. Cotman, Daniele Piomelli

Sanders-Brown Center on Aging Faculty Publications

Reduced brain levels of docosahexaenoic acid (C22:6n-3), a neurotrophic and neuroprotective fatty acid, may contribute to cognitive decline in Alzheimer's disease. Here, we investigated whether the liver enzyme system that provides docosahexaenoic acid to the brain is dysfunctional in this disease. Docosahexaenoic acid levels were reduced in temporal cortex, mid-frontal cortex and cerebellum of subjects with Alzheimer's disease, compared to control subjects (P = 0.007). Mini Mental State Examination (MMSE) scores positively correlated with docosahexaenoic/α-linolenic ratios in temporal cortex (P = 0.005) and mid-frontal cortex (P = 0.018), but not cerebellum. Similarly, liver docosahexaenoic acid content was lower in Alzheimer's …

Insulin Resistance In African-American And Caucasian Women: Differences In Lipotoxicity, Adipokines, And Gene Expression In Adipose Tissue And Muscle, Latasha M. Smith, Aiwei Yao-Borengasser, Tasha Starks, Mark Tripputi, Philip A. Kern, Neda Rasouli

Clinical and Translational Science Faculty Publications

Objectives: We tested whether African-American (AA) women are different from Caucasian women in regard to lipotoxicity, adipokines, and gene expression in adipose tissue and muscle.

Design: Insulin sensitivity (S_I), plasma adipocytokine levels, intramyocellular lipid (IMCL), and the expression of candidate genes in adipose tissue and muscle were measured in AA and Caucasian women.

Setting: This study was performed in an ambulatory general clinical research center.

Subjects: Subjects were healthy, nondiabetic AA and Caucasian women.

Interventions: There were no interventions.

Main Outcome Measures: Comparison of S_I, IMCL, plasma adiponectin, and the expression of candidate genes regulating adipogenesis, …

Forage News [2010-07], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Wins Awards at Recent American Forage & Grassland Council Annual Meeting
• Kentucky Host International Meeting
• Dr. Smith Becomes Professor Smith
• Tom Keene Re-elected to Forage Foundation Executive Committee
• Getting through the Summer Slump
• National Hay Association Convention
• Heart of America Grazing Conference Coming to Kentucky
• Clover Coming up in Alfalfa Fields
• Selecting Summer Annual Forage Grasses
• Abundance of Clover in 2010
• Heat Damage to Moist Hay
• The Economics of Grazing, Organic, and Confinement Dairy Farms

Recent Trends In Exposure To Secondhand Smoke In The United States Population, Chieh-I Chen, Tanya Burton, Christine L. Baker, Vera Mastey, David Mannino

Preventive Medicine and Environmental Health Faculty Publications

BACKGROUND: Previous research using the National Health and Nutrition Examination Surveys (NHANES) data documented a significant downward trend in secondhand smoke (SHS) exposure between 1988 and 2002. The objective of this study was to assess whether the downward trend in exposure continued from 2001 through 2006.

METHODS: We analyzed data from the 2001-2006 NHANES to estimate exposure of nonsmokers to SHS. Geometric means of serum cotinine levels for all nonsmokers were computed.

RESULTS: Overall serum cotinine levels (95% Confidence Intervals) in 2001-2002, 2003-2004, and 2005-2006 were 0.06 ng/mL (0.05-0.07), 0.07 ng/mL (0.06-0.09), and 0.05 ng/mL (0.05-0.06), respectively. Subgroup analysis by …

Policy Analysis: Is There A Relationship Between Required Physical Education In Lower Grades And Adolescent Obesity Rates? A Kentucky Case Study, David A. Gross

Center of Excellence in Rural Health Presentations

Childhood obesity has emerged as a national epidemic − and one with serious short- and long-term personal health, medical system and economic consequences. The prevalence of obesity more than doubled among U.S. children ages 6 to 11 between 1980 and 2006, while the rate for those from ages 12 to 19 more than tripled. This study analyzes whether required physical education in elementary and/or middle school is associated with lower rates of obesity among U.S. high school students, with an emphasis on those living in Kentucky.

Socio-Environmental And Endocrine Influences On Developmental And Caste-Regulatory Gene Expression In The Eusocial Termite Reticulitermes Flavipes, Matthew R. Tarver, Xuguo Zhou, Michael E. Scharf

Entomology Faculty Publications

BACKGROUND: Strict regulation of caste differentiation, at the molecular level, is thought to be important to maintain social structure in insect societies. Previously, a number of extrinsic and intrinsic factors have been shown to influence caste composition in termite colonies. One important factor is the influence of nestmates; in particular, soldier termites are known to inhibit hormone-dependent worker-to-soldier differentiation. However, soldier influences on nestmates at the molecular level are virtually unknown. Here, to test the hypothesis that soldiers can influence nestmate gene expression, we investigated the impact of four treatments on whole-body gene expression in totipotent Reticulitermes flavipes workers: (i) …

Forage News [2010-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 31st Kentucky Alfalfa Conference Date Set
• 30th Alfalfa Conference Proceedings and Photos
• Warm Season Grass Variety Reports
• WEG Forage Supplies
• Kentucky Grazing School April 14-15
• Windrow Spacing and Conditioners Aid in Curing Process
• Preseason Hay Equipment Maintenance
• Overview of BMR Sudangrasses and Sorghums
• Switchgrass Moved by Barge to Power Plan

Bodies Of Knowledge: Considerations Of Science, Exercise, Food And Body Politics, Ben Agger

disClosure: A Journal of Social Theory

No abstract provided.

Much Ado About Mutton: An Interview With Deborah Gewertz And Frederick Errington, Rebecca Lane, Christine Smith

disClosure: A Journal of Social Theory

No abstract provided.

Care, Cure, And Control: A Politics Of Dietetics, Rick Dolphijn

disClosure: A Journal of Social Theory

No abstract provided.

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• River Basins of Kentucky, Dan Carey, Kentucky Geological Survey
• An Initial Prioritization Approach for Potential Agricultural Best Management Practice Implementation Based on Subwatershed Indicators and Expert Knowledge, Brian Lee and Corey Wilson, Dept Landscape Architecture, UK
• Locating Karst Conduits in Cane Run Watershed of Central Kentucky Using Electrical Resistivity Methods, Junfeng Zhu and others, Kentucky Geological Survey
• Identification of DNA Biomarkers for Determining Sources of Fecal Pollution in Water, Rick Fowler and others, WATERS Laboratory, WKU
• Integrating Participatory Communication and Structured Public Involvement Processes to Better Address Superfund Issues: The Paducah Gaseous Diffusion Plant Future State …

Eicosapentaenoic Acid Preserves Diaphragm Force Generation Following Endotoxin Administration, Gerald S. Supinski, Jonas Vanags, Leigh Ann Callahan

Internal Medicine Faculty Publications

INTRODUCTION: Infections produce severe respiratory muscle weakness, which contributes to the development of respiratory failure. An effective, safe therapy to prevent respiratory muscle dysfunction in infected patients has not been defined. This study examined the effect of eicosapentaenoic acid (EPA), an immunomodulator that can be safely administered to patients, on diaphragm force generation following endotoxin administration.

METHODS: Rats were administered the following (n = 5/group): (a) saline, (b) endotoxin, 12 mg/kg IP, (c) endotoxin + EPA (1.0 g/kg/d), and (d) EPA alone. Diaphragms were removed and measurements made of the diaphragm force-frequency curve, calpain activation, caspase activation, and protein carbonyl …

Forage News [2010-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Alfalfa Conference Reflections After 30 Years
• Kentucky Alfalfa Awards
• Hay Winners
• UK Grazing Alfalfa DVD on YouTube
• Kentucky Grazing School April 14-15
• USDA Issues Final Rule on Organic Access to Pasture
• UK Forage Variety Test Available
• Points to Ponder