Digital Commons Network^™

Insights Into Key Gene Regulatory Networks In Borrelia Burgdorferi, William Kenneth Arnold

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Gene regulatory networks are composed of interconnected regulatory nodes created by regulatory factors of multiple types. All organisms finely tune gene expression in order to adapt to and survive within their current niche. Obligate parasitic bacteria are under extreme pressure to quickly and appropriately adapt their gene regulatory programs in order to survive within their given host. Borrelia burgdorferi is one such organism and persists in nature by alternating between two hosts; Ixodes spp. ticks and small vertebrate animals. These two hosts represent drastically different environments; requiring a unique gene regulatory program to survive and transmit between them. Microbiologists have …

Emerald Ash Borer (Coleoptera: Buprestidae) Host Expansion; Trading An Optimal Host For Enemy Free Space?, David G. Olson

Theses and Dissertations--Entomology

Emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae), is an aggressive invader from Asia that has killed millions of trees in North America. Recently EAB has been documented developing in a novel host, white fringetree, Chionanthus virginicus. I evaluated larval performance in two common ash species and white fringetree by infesting excised bolts with emerald ash borer eggs. In addition I evaluated several plant characteristics to determine which most influence larval development. I also conducted choice and no choice assays using the classical biological control agent, Tetrastichus planipennisi (Hymenoptera: Eulophidae), to assess its ability to locate larval EAB in …

Analyzing Physical Education Teacher Behavior Using Systematic Observation, Seth T. Eckler

Theses and Dissertations--Education Sciences

Introduction: Experts in the field have advocated that quality physical education (QPE) is the centerpiece of a comprehensive school physical education program (CSPAP). Evidence-based programs and instructional models have shown great promise in increasing the physical activity (PA) of today’s youth. However, little is known about the specific impact of teaching behaviors (TB) used within these programs and their impact on student outcomes. Therefore, the aim of this study was to use a systematic observation method that is primarily focused on analyzing TBs that are displayed in class, identify TBs that may contribute to positive student activity outcomes, and make …

Physio-Chemical And Functional Properties Of Nine Proso Millet Cultivars, Manjot Singh, Akinbode A. Adedeji, Dipak Santra

Biosystems and Agricultural Engineering Faculty Publications

Evaluation of the postharvest properties of nine proso millet cultivars was carried out to determine their physical and engineering properties, which are very useful for designing appropriate systems for process operations such as sorting, drying, heating, cooling, and milling. Nine cultivars of proso millet comprising waxy and non-waxy types, namely Cope, Earlybird, Huntsman, Minco, Plateau, Sunrise, Rise, Dawn, and Panhandle, were obtained from the Panhandle Research and Extension Center, University of Nebraska, Scottsbluff. Results showed significant (p < 0.05) differences in their physical properties, such as sphericity, volume, bulk density, porosity, and angle of repose, which ranged from 0.86 to 0.91, from 3.94 to 5.14 mm³, from 765.49 to 809.67 kg m^-3, from 42.49% to 44.20%, and from 22.98° to 25.74°, respectively. The cultivars were …

Effects Of Very Short-Term Dynamic Constant External Resistance Exercise On Strength And Barbell Velocity In Untrained Individuals, M. Travis Byrd, Haley C. Bergstrom

Kinesiology and Health Promotion Faculty Publications

This study examined the effect of an upper body dynamic constant external resistance (DCER) exercise (barbell bench press [BP]), using the very short-term training (VST) model on strength and barbell velocity. Ten (5 females, 5 males) subjects (mean ± SD age: 21.4 ± 2.8 yrs; height: 1.75 ± 0.12 m; body mass: 83 ± 8.8 kg) completed two pre-test visits (pre-test 1 and pre-test 2) to serve as the within subjects control, three training visits, and one post-test visit. The subject’s 1 repetition maximum (1RM) for the BP as well as the mean (BP_MV) and peak (BP_{PV …}

The Opioid Epidemic And Rural American: Why The Usda Should Lead The Response, Lexy Gross

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Investigating The Effects Of Homocysteine As An Agonist On Invertebrate Glutamatergic Synapses, Elizabeth Grau, Alexandra E. Stanback, Alec Bradley, Danielle Cantrell, Samantha Eversole, Carolyn Grachen, Kaylee Hall, Danielle Hawthorne, Claire Kinmon, Paula Ortiz Guerrero, Bhavik Patel, Kaitlyn Samuels, Chinni Suryadevara, Gia Valdes, Samuel Wycoff, Robin L. Cooper

Biology Faculty Publications

Hyperhomocysteinemia (HHcy) in mammals can produce neurological deficits, such as memory loss. The cause of the neurological issues is assumed to be due to homocysteine (HCY) binding to glutamatergic receptors in the central nervous system (CNS). High levels of HCY in the CNS are also associated with Amyotrophic Lateral Sclerosis (ALS) and Parkinson’s disease. Thus, understanding the detailed mechanisms of HCY in model preparations could be useful in developing potential treatments to neurodegenerative diseases with overlapping symptoms to HHcy. The aim of this study is to investigate the efficacy of HCY as an agonist at glutamatergic synapses in invertebrates. The …

Mom To Mom: Online Breastfeeding Advice, Jennifer D. Furkin

Theses and Dissertations--Communication

Exploring online support groups has gained more and more popularity in the last decade. Investigating the type of support messages users send each other has broadened the already extensive social support framework built in the last forty years. Mothers utilize online support for various topics, and a very common topic is breastfeeding. The perception of breastfeeding has changed throughout history with shifting beliefs and societal norms coupled with solid facts about its importance in the sustaining of infants. Online breastfeeding support has been previously explored through the categorization of types of support and themes within the interactions. This study extended …

Quitting Together: Formative Research To Develop A Social Marketing Plan For Smoking Cessation Among Women In A Residential Treatment Facility For Substance Abuse Recovery, August D. Anderson

Theses and Dissertations--Communication

Both smoking addiction and illicit substance abuse are prevalent issues in the United States today. Furthermore, these are issues that have significant impact on women’s health and mental state. Despite research that shows that smoking cessation coupled with substance abuse recovery can decrease likelihood of relapse post-recovery, few substance abuse recovery facilities today offer smoking cessation programming options. To address the issue of smoking addiction on top of substance abuse recovery, formative research was conducted through this study to determine the underlying causes of smoking habits coupled with recovery efforts and the attitudes. Through focus group sessions with women in …

Not Your Grandma’S Iud: Identifying Memorable Messages About Larcs, Carina Mazariegos Zelaya

Theses and Dissertations--Communication

Reducing the percentage of unwanted pregnancies has been one of the top objectives of the national health promotion "Healthy People" since the early 1980s. As the increase of unwanted pregnancies continues, research is necessary to uncover factors that influence the decisions women make about their contraceptive methods. The current study examines the topics and the sources of memorable messages regarding LARCs. To address the research questions, this study will analyze interviews conducted with young women about memorable messages regarding LARC methods. This study is a formative research of memorable messages in the context of contraceptive methods, specifically long-acting reversible contraceptives …

The Impact Of Insulin Dysregulation On Protein Metabolism In Horses, Caroline Margot Marcelle Loos

Theses and Dissertations--Animal and Food Sciences

Insulin plays a vital role in whole-body metabolism and provides a major anabolic stimulus for cellular signaling pathways, including those involved in the metabolism of glucose and protein. Consequently, insulin dysregulation (ID) is known to alter molecular signal transduction in insulin-sensitive tissues such as skeletal muscle, thereby disrupting glucose metabolism and compromising protein synthetic capacity. Our first objective was to induce ID in healthy horses by administering dexamethasone (DEX), a potent glucocorticoid, for 21 days. We evaluated the effects on insulin-stimulated muscle protein signaling components involved in the mammalian target of rapamycin (mTOR) pathway. DEX-induced ID reduced insulin-stimulated activation of …

Factors Influencing Phosphorus Excretion By Horses, Ashley Lauren Fowler

Theses and Dissertations--Animal and Food Sciences

Excessive phosphorus (P) excreted by animals can affect water quality and cause eutrophication. Better understanding of factors that influence P utilization and excretion in horses may reduce the environmental impact of P. Two animal experiments were conducted that examined P excretion by horses. The efficacy of titanium dioxide as an external marker to calculate digestibility was studied concurrently with both animal experiments. Additionally, pasture P concentrations were evaluated over the growing season using near-infrared spectroscopy (NIRS). Experiment 1 examined P excretion by post-lactational mares fed a low P diet immediately prior to weaning compared to non-lactating controls fed an adequate …

On-Farm Utilization Of Precision Dairy Monitoring: Usefulness, Accuracy, And Affordability, Elizabeth A. Eckelkamp

Theses and Dissertations--Animal and Food Sciences

Precision dairy monitoring is used to supplement or replace human observation of dairy cattle. This study examined the value dairy producers placed on disease alerts generated from a precision dairy monitoring technology. A secondary objective was calculating the accuracy of technology-generated disease alerts compared against observed disease events. A final objective was determining the economic viability of investing in a precision dairy monitoring technology for detecting estrus and diseases.

A year-long observational study was conducted on four Kentucky dairy farms. All lactating dairy cows were equipped with a neck and leg tri-axial accelerometer. Technologies measured eating time, lying time, standing …

Dairy Cattle Hoof Disease Costs And Considerations For Prevention, Karmella A. Dolecheck

Theses and Dissertations--Animal and Food Sciences

Lameness is considered one of the most important health and welfare issues in the dairy industry. Understanding the total cost per case of disease-specific lameness can help producers select better treatment, prevention, and control strategies for their herds. The first objective of our research was to calculate the costs associated with 3 lameness causing hoof diseases: digital dermatitis, sole ulcer, and white line disease. To accomplish this, a survey of hoof health professionals (hoof trimmers and veterinarians) was conducted to identify treatment related expenditures per case. Data from the hoof trimmer responses to the survey and previously published research were …

An Evaluation Of Physiological And Behavioral Indicators Of Postpartum Diseases And Heat Stress In Dairy Cows, Amanda Renee Lee

Theses and Dissertations--Animal and Food Sciences

Precision dairy monitoring technologies can be used to monitor changes in physiology and behavior associated with transition period postpartum diseases and heat stress. Research Objective One was to evaluate how traditional visual examination, body condition, and locomotion with and without blood, milk, and urine variables and precision dairy monitoring technologies determine variable association with hyperketonemia, metritis, mastitis, hypocalcemia and retained placenta,. This was accomplished by monitoring cows 2 weeks before calving to 3 weeks after calving for any postpartum diseases using daily visual examination and automatically detected variables including activity, milk yield, milk components, lying behavior, feeding behavior, rumination time, …

The Effects Of Housing On Dairy Cow Comfort, Immune Function, Stress, Productivity, And Milk Quality, Matthew Richard Borchers

Theses and Dissertations--Animal and Food Sciences

Mastitis and milk quality affect every dairy farmer across the globe. Sand bedded freestalls are the industry standard for cow comfort, welfare, and the control of environmental mastitis. Compost bedded packs may be a viable alternative to the sand bedded freestall.

Compost bedded packs are maintained at a consistent level of moisture, nutrients, and aeration to favor compost microorganisms. Greater bacteria counts in bedding have traditionally been associated with increased mastitis rates and mastitis pathogens can be found in the pack and on the teats of cattle housed in even well managed compost bedded pack barns. In spite of this, …

Assessment Of The Serum Amyloid A Assay For Diagnosing Disease In Neonatal Foals, Samantha W. Strouss

Theses and Dissertations--Animal and Food Sciences

Diagnosing disease in equine neonates poses a challenge for the equine industry because of the nonspecific manifestations of many diseases and the rapid deterioration that occurs. The differential diagnostic procedure requires many laboratory tests, whose results take days to receive. Serum amyloid A (SAA) is the only major acute phase protein identified in the horse; it exists in low levels in the healthy horse and increases over 100 fold in response to inflammatory stimulus 6-8 hours post stimulus. A point of care test allows veterinarians to obtain a SAA concentration within minutes that indicates the existence of infection. Being able …

Whole-Body Protein Metabolism In Mature And Growing Horses Receiving Predominantly Forage Diets, Sophie A. Stratton

Theses and Dissertations--Animal and Food Sciences

There has been limited investigation as to whether a predominantly forage-based diet can provide adequate amounts of limiting amino acids (AA) to horses. The first objective was to determine if AA supplementation of AA believed to be limiting to protein synthesis in forage-based diets would affect measures of whole-body protein metabolism in sedentary mature horses. The effect of forage type (timothy or alfalfa) and AA supplementation (lysine, threonine or histidine) on plasma urea nitrogen (PUN) and AA concentrations and measures of whole-body phenylalanine kinetics were evaluated. There was no effect of either forage type or AA supplement on rates of …

Music Training As A Neuro-Cognitive Protector For Brain Aging: Cognitive And Neuropsychological Profiles In Professional Musicians, Catherine E. Schneider

Theses and Dissertations--Gerontology

The proportion of older adults living with cognitive impairments is increasing rapidly. This shift will likely increase mortality rates, reduce perceived quality of life, and cause economic burden to patients and health care systems. Currently evidence of highly effective and noninvasive interventions that prevent or slow the onset of cognitive impairment are limited. This study aims to better understand what drives cognitive aging variability among musicians versus non-musicians. Music playing has been shown to improve brain and cognitive functions by engaging networks of brain areas, simultaneously involving cortical mechanisms associated with executive, high-level cognitive and motor functions, and multiple sensory …

The Impacts Of Honey Bee Queen Stress On Worker Behavior And Health, Sarah R. Preston

Theses and Dissertations--Entomology

Pesticides, poor nutrition, parasites and diseases work synergistically to contribute to the decline of the honey bee. Heritable sub-lethal behavior/immune effects may also contribute to the decline. Maternal stress is a common source of heritable immune/behavior deficits in many species. A stressed honey bee queen has the potential to pass such deficits on to worker bees. Using a repeated measures design, this study will determine whether the health of worker bee is reduced by a cold stress on the queen by analyzing egg hatch rate and protein content, emergence rate, and adult aggression and immune function for offspring laid before …

There’S Nowhere To Hide: Artificial Intelligence And Privacy In The Fourth Industrial Revolution, Sarah Denton, Eleonore Pauwels, Yujia He, Walter G. Johnson

Patterson School of Diplomacy and International Commerce Faculty Publications

At its core, artificial intelligence (AI) optimizes data. Machine-learning algorithms, one component of AI, are trained using massive datasets curated by humans to predict various aspects of our daily lives. Such predictive intelligence could be a positive force amplified by continued decentralization of the technology. Or, ubiquitous cognition and surveillance could be a disruptive force amplified by the unregulated proliferation of AI technologies. Yet, though we will certainly see more and more AI systems being integrated into every facet of our infrastructures, homes, and bodies, the proliferation of AI technology isn’t the problem. A privacy-security quagmire arises from the interconnectivity …

Implementation Of An Evidenced-Based Opioid Treatment Program To Improve Maternal-Child Outcomes In Eastern Kentucky, Jordan Rice

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The Kentucky River Area District Health Department proposes implementation of CHARM-Kentucky, a treatment program for pregnant women with opioid use disorder (OUD), in eastern Kentucky. Over 115 Americans die each day from an opioid overdose, resulting in approximately 42,000 deaths in 2016. In addition to an overall increase in opioid use in recent years, the use of opioids in pregnancy has also increased from 1.19 per 1,000 hospital births in 2000 to 5.63 in 2009. Opioid use during pregnancy results in poor neonatal outcomes including: low birth weight, preterm delivery, small head circumference, increased child maltreatment, and risk for long-term …

Epidemiology Of Young Adult Renal Cell Carcinoma In Kentucky: Incidence, Clinicopathologic Features, And Cancer-Specific Survival, Jason R. Bylund

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Introduction:

Renal cell carcinoma (RCC) is an important cause of morbidity and mortality in our country. Previously reported data suggest that the overall incidence of RCC continues to rise and may be accelerating even faster among younger adult patients.

Methods:

Data for analysis included the combined NPCR-SEER USCS public use database and the Kentucky Cancer Registry dataset. Incidence rates and trends by age group and location were calculated and tested. Clinicopathologic features of disease among Kentucky cases were analyzed by age group. Cancer-specific survival (CSS) analysis was performed, along with Cox proportional hazards regression to test for association of younger …

An Assessment Of Agreement In Detection Methods For Histophilus Somni In Bovine Lungs, Laura Michelle Arnold

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Objectives: To examine the inter-laboratory agreement between multiplex realtime quantitative polymerase chain reaction (qPCR) assay results and aerobic culture results on bovine lung samples for detection of Histophilus somni (HS), and to assess associations of laboratory-derived factors on test agreement.

Methods: A survey of records from the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) was conducted to evaluate test results from grossly pneumonic bovine lung samples submitted during the period April 1, 2015 through August 31, 2018. Cohen’s kappa coefficient with 95% confidence interval (CI) was calculated to describe the extent of agreement. Animal, environmental and laboratory factors were examined …

The 2015 University Of Missouri Protests And Their Lessons For Higher Education Policy And Administration, Ben Trachtenberg

Kentucky Law Journal

No abstract provided.

Serum Tumor Necrosis Factor-Alpha Associates With Myocardial Oxygen Demand And Exercise Tolerance In Postmenopausal Women, Stephen J. Carter, David R. Bryan, William H. Neumeier, Stephen P. Glasser, Gary R. Hunter

Internal Medicine Faculty Publications

The functional implications of serum tumor necrosis factor-alpha (TNF-α), a marker of oxidative stress, on hemodynamic parameters at rest and during physical exertion are unclear. The aims of this investigation were to examine the independent associations of TNF-α on myocardial oxygen demand at rest and during submaximal exercise, while also evaluating the association of TNF-α on exercise tolerance. Forty, postmenopausal women, provided blood samples and completed a modified-Balke protocol to measure maximal oxygen uptake (VO_2max). Large artery compliance was measured by pulse contour analyses while rate-pressure product (RPP), an index of myocardial oxygen demand, was measured at rest …

Categorizing A Continuous Predictor Subject To Measurement Error, Betsabé G. Blas Achic, Tianying Wang, Ya Su, Victor Kipnis, Kevin Dodd, Raymond J. Carroll

Statistics Faculty Publications

Epidemiologists often categorize a continuous risk predictor, even when the true risk model is not a categorical one. Nonetheless, such categorization is thought to be more robust and interpretable, and thus their goal is to fit the categorical model and interpret the categorical parameters. We address the question: with measurement error and categorization, how can we do what epidemiologists want, namely to estimate the parameters of the categorical model that would have been estimated if the true predictor was observed? We develop a general methodology for such an analysis, and illustrate it in linear and logistic regression. Simulation studies are …

Evaluation Of Barriers For Small-Scale Fruit And Vegetable Growers In Kentucky, Amanda Schroeder Hege

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The food system in the United States has witnessed significant challenges resulting in food security and safety concerns, environmental damage, economic distress, and a decline in our population’s health. While the last fifty years showed a drop in land and workforce dedicated to farming, industrialized farms are producing an overabundance of cheap corn that directly supplies inexpensive, unhealthy foods leading to American’s diets falling short of recommendations for good health, thus contributing to the obesity epidemic. This study utilizes an upstream approach to learn from farmers’ ability to grow good food that promotes healthy people, environments, and communities. Specifically, the …

The Relationship Between Access To Healthcare And Heart Disease Mortality In Kentucky, 2012-2014, Nima Farsi

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Cardiovascular disease is the cause for a large portion of the mortalities in the United States. Furthermore, Kentucky has one of the highest heart disease mortality rates out of all 50 U.S. States. This study hypothesized that heart disease mortality in Kentucky counties has a positive relationship with lack of accessibility to healthcare. Furthermore, this ecological study hypothesized that because there is no available usual source of a healthcare providers and ultimately a lack of accessibility to receive diagnostic/preventative services for this population, the high rate of cardiovascular disease mortality in Kentucky is dependent on access to healthcare services.

This …

Implementing The National Diabetes Prevention Program In Mason County, Kentucky, Cassandra Yelton

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The National Diabetes Prevention Program (DPP) is sponsored by the Centers for Disease Control and Prevention (CDC). The program’s goal is to reduce the incidence of diabetes through dietary modification and physical activity. On one hand, the DPP is currently offered by various organizations through the country. On the other hand, the DPP is mostly unavailable in rural areas, especially the Diabetes Belt. The Diabetes Belt is in the eastern Appalachian region of the United States. People who live in this area of the country are more likely to develop diabetes than those who do not. Mason County, Kentucky has …