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Siderophore-Mediated Zinc Acquisition Enhances Enterobacterial Colonization Of The Inflamed Gut, Judith Behnsen, Hui Zhi, Allegra T. Aron, Venkateswaran Subramanian, William Santus, Michael H. Lee, Romana R. Gerner, Daniel Petras, Janet Z. Liu, Keith D. Green, Sarah L. Price, Jose Camacho, Hannah Hillman, Joshua Tjokrosurjo, Nicola P. Montaldo, Evelyn M. Hoover, Sean Treacy-Abarca, Benjamin A. Gilston, Eric P. Skaar, Walter J. Chazin, Sylvie Garneau-Tsodikova, Matthew B Lawrenz, Robert D Perry, Sean-Paul Nuccio, Pieter C. Dorrestein, Manuela Raffatellu

Pharmacy Nuclear Magnetic Resonance Center Faculty Publications

Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or “Nissle”) exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin’s affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the …

Forage Production From Perennial Vs. Annual Crop R~ On In Sandy Soils In Egypt, Z. Abdelhalim, O. Niemeläinen, I. A. Hanna, A. M. Rammah

IGC Proceedings (1977-2023)

Forage production was studied on newly reclaimed sandy soil in Egypt trom perennial lucerne (Medicago sativa L.) and two annual crop rotations. The cropping systems were: A) lucerne, B) berseem clover (Trifolium alexandrinum L.) in the winter followed by pearl millet (Pennisetum glaucum R. Br. Emend. Stantz.) in the summer and C) triticale (XX Triticosecale Wittm.) (forage cut + grain harvest) in the winter followed by maize (Zea mays L.) (grain + stover) in the summer. Mean annual dry matter yields (t ha^-1 ) were 20.65, 26.59 and 27.48 from A, B and C, respectively. However, …

Forage Quality Evaluation Of Chopped Elephantgrass Hay Through Intake And Digestibility Trial, E. M. De Aguiar, G. F. Da C. Lima, L. G. Da Paz

IGC Proceedings (1977-2023)

The research was carried out to evaluate forage quality of chopped elephantgrass (Pennisetum purpureum Schum.) hay and to define a proper stage for cutting and haymaking of this grass. Chemical composition, voluntary intake, and apparent digestibility of the hays were studied at five different growth stages (30, 48, 63, 76, and 91 days). Twenty-five common wethers were utilized in a completely randomized experimental design. Dry matter (DM) voluntary intake was not different (P> 0.05) from 30 to 63 days of regrowth and from 63 to 91 days and comprised 3.9; 3.5; 3.2; 2.1; and 2.4% of animal liveweight, respectively …

Berseem Clover In Binary Mixtures With Oats, Triticale Or Barley For Silage And Late Season Grazing, S. M. Ross, J. R. King

IGC Proceedings (1977-2023)

An experiment was conducted at northerly latitudes with berseem clover (Trifolium alexandrinum L) in binary mixtures with oats (Avena sativa L.), barley (Hordeum vulgare L.) and triticale (X Triticosecale Wittmack). The effects of cereal species, variety and density were assessed for a two cut harvest. Total dry matter yields were 11.7 to 14.5 t ha^-1, with 7.8 to 10.2 t ha^-1 as silage, and 2.0 to 6.0 t ha^-1 as berseem regrowth. Berseem regrowth added an average of 4.0 t ha^-1 in forage yield. Total yields did not differ with cereal …

Exclusive Breastfeeding And Professionals From The Family Health Strategy, Marialda Moreira Christoffel, Ana Leticia Monteiro Gomes, Cássia Leoneuza Augusto Julio, Julia Florentino De Barros, Elisa Da Conceição Rodrigues, Fernanda Garcia Bezerra Góes, Ana Maria Linares

Nursing Faculty Publications

OBJECTIVES: to analyze the perception of health professionals about exclusive breastfeeding in Family Health Strategy units in the city of Macaé.

METHODS: qualitative study carried out in four units of the Family Health Strategy in the city of Macaé, Rio de Janeiro. Thirty health professionals were interviewed from March to May 2019. Textual contents were processed in the IRaMuTeQ^® software by the Descending Hierarchical Classification.

RESULTS: professionals use different strategies for actions to promote, protect and support breastfeeding in prenatal consultations, but social and cultural determinants are important issues that interfere in this process, the involvement of the family …

Transgenic Maize Hybrid Crop Residues: Nutritive Value And Their Effects On Performance Of Grazing Beef Cows, S. K. Barnhart, J. R. Russell, M. J. Hersom, D. E. Farnham

IGC Proceedings (1977-2023)

Several maize (Zea mays) hybrids, including three transgenic hybrids containing Bacillus thuringiensis (Bt) enzymes (Bt-maize hybrids), were planted in a field study. Mature, midgestation, beef cows (Bos tarus) strip-grazed post-harvest residues as paddocks over 126 days. Body condition-scores and weights were monitored. Forage yield, weathering losses and forage composition were monitored on grazed and ungrazed areas. Forage selection and intake were estimated. Grain yields, dropped grain, initial crop residue concentrations of dry matter (DM), organic matter (OM) and in vitro digestible organic matter (IVDOM) yields were similar among hybrids. Hybrids varied in infestation of maize borers, …

Supplementation Of Temperate Pastures, D. H. Rearte, G. A. Pieroni

IGC Proceedings (1977-2023)

Generally in temperate regions, beef and milk were produced on extensive systems with forage from perennial pasture and natural grassland being the main component of animal diet. Supplementation on pasture was usually applied strategically to supply nutrients to grazing cattle only when forage availability was not enough to satisfy animal’s requirements. At present beef and dairy production systems have become more intensive and even when forage from pasture continue being the main component of the diet higher level of different type of supplement are fed to the animals. Intensive systems are characterized by a higher stocking rate capable of consuming …

Feeding Of Conserved Forage - Implications To Grassland Management And Production, C. W. Holmes, P. N. P. Matthews

IGC Proceedings (1977-2023)

This review will focus on the use of conserved forages in pastoral dairy farm systems; especially on their conservation as an aid to good grazing management during periods of rapid pasture growth, and on their consumption as an aid to good feeding management and grazing management during periods of slow pasture growth. Conservation of pasture, in order to move it from one time to another later time, always involves “costs”, either financial expenditure or physical losses (of dry matter/quality) or both, which must be evaluated in the whole system.

Most of the data is drawn from New Zealand and Australia, …

High Moisture Sorghum Grain Silage: Effects Of Tannin Content And Urea Treatment On The Performance Of Dairy Cows, L. A. Romero, E. A. Comerón, O. A. Bruno, A. R. Castillo, M. C. Gaggiotti

IGC Proceedings (1977-2023)

Grain sorghum silage tannin content effect was evaluated on milk production and chemical composition of Argentinean Holstein bred cows, and it are described dry matter (DM) and crude protein (CP) in situ digestion parameters and effective degradability. The base diet was constituted by alfalfa pasture, maize silage, and a protein – mineral supplement, differing in three treatments based on high moisture grain silage characteristic: LTS= Low tannin grain sorghum, HTS= High tannin grain sorghum, and HTSu= High tannin sorghum plus the addition of urea. Grain sorghum silage tannin content affects milk production, without significant alteration of chemical composition. Beside the …

Comparative Growth Performance Of West African Dwarf Goat Supplemented With Calliandra Calothyrsus, Leucaena Leucocephala, Or Cotton Seed Cake In West Cameroon, E. Tedonkeng Pamo, G. D. Assontia, C. Njehoya

IGC Proceedings (1977-2023)

A comparative growth performance study of African Dwarf Goats supplemented with Calliandra calothyrsus, Leucaena leucocephala or cotton seed cake was carried out on farm around Dschang and in the University Experimental Farm. Goats between 12 and 16 months of age were divided into 4 groups of 11 goats each with one group kept as a control. The supplemented animals received respectively 390 g of L. leucocephala, 380 g of C. calothyrsus (fresh matter) and 88.8 g of cotton seed cake each, corresponding to 6 g of nitrogen per animal per day. The animals were weighed every 21 days …

The Effect Of Browse Species When Fed As A Supplement To Low Quality Native Grass Hay On Animal Performance, A. Elgersma, J. F. Oerlemans, D. B. Coates

IGC Proceedings (1977-2023)

A pen trial was conducted to measure the impact of some browse species when fed as a supplement on the nitrogen (N) and dry matter intake of cattle receiving low quality native grass hay. There were 8 treatments consisting of a nil-supplement control treatment, a urea supplement control, a green oaten hay control and 5 treatment groups based on browse supplements (Albizia lebbeck, Carissa spp. and Bursaria spp.). Treatment effect on increased native pasture intake was related to the amount of supplementary nitrogen (N), such that there was a significant linear relationship between dry matter intake of native …

Effects Of Different Levels Of Energy Supplementation On Growth Of Grazing Holstein Heifers, L. P. Novaes, C. E. Polan

IGC Proceedings (1977-2023)

Growth of Holstein heifers was studied for 1987 grazing season to evaluate pasture-drylot systems for raising dairy replacements. Orchardgrass (Dactylis glomerata L), tall fescue (Festuca arundinacea Schreb), bluegrass (Poa pratensis L) and white (Trifolium repens L) and red clover (Trifolium pratense, L) were the principal components of the grass-legume mixture in six paddocks of 1.5 ha each. Carrying capacity was estimated at six animals/ha (3.1 animal units) and excess forage was harvested as hay to control forage growth and quality. Grazing days were 140 days. None or 2.0 kg/d …

Fatty Acid Composition And Eating Quality Of Muscle From Steers Offered Grazed Grass, Grass Silage Or Concentrate-Based Diets, A. P. Moloney, P. French

IGC Proceedings (1977-2023)

The effects of grazed grass, grass silage or concentrates on the eating quality and fatty acid composition of intra-muscular fat of steers fed to achieve similar carcass growth rates were investigated. Fifty steers were assigned to one of five dietary treatments. The experimental rations offered daily for 85 days pre-slaughter were (a) grass silage plus 4 kg concentrate, (b) 8 kg concentrate plus 1 kg hay, (c) 6 kg grazed grass dry matter (DM) plus 5 kg concentrate, (d) 12 kg grazed grass DM plus 2.5 kg concentrate or (e) 22 kg grazed grass DM. Decreasing the proportion of concentrate …

Supplementation Of Lactating Cows Grazing Stargrass (Cynodon Nlenfuensis Var. Nlenfuensis) With Conjugated Linoleic Acid: Effects On Persistency And Estimated Net Energy Balance, S. R. Medeiros, D. E. Oliveira, L. J. M. Aroeira, M. Mcguire, D. P. D. Lanna

IGC Proceedings (1977-2023)

Thirty crossbread cows grazed stargrass (Cynodon nlenfuensis var. nlenfuensis) receiving 4kg/d of a supplement formulated to provide 115% of metabolizable protein requirements from the 4^th to the 11^th week of lactation. The cows received either 150g/head/day of Megalac (Control) or 150g/head/day of a Ca-protected conjugated linoleic acid (CLA, Church & Dwight). Residual effects of treatment were evaluated in weeks 12^th and 13^th. Milk production increased in CLA treated cows (P< 0.05). CLA decreased milk fat content (P< 0.0001) and yield (P< 0.01). Protected CLA increased protein content (P< 0.01) and yield (P< 0.01). The CLA residual effect on milk fat ends within one week after withdrawal but the benefits on milk yield and total solids production continued, due to the increased persistency. The estimated energy balance and intake were not greatly affected by treatment, as treated cows had higher milk volume and had higher protein productions.

Use Of Supplements For Maintenance Of Nelore Bullocks In Brachiaria Decumbens, During The Dry Season In Acre, Brazilian Amazon, J. C. Carneiro, Judson F. Valentim, L. A. R. Valle

IGC Proceedings (1977-2023)

This study had the objective of evaluating animal performance and net economic returns of Nelore bullocks receiving: 1) mineral mixture (MM), 2) mineral mixture + urea (MMU), and 3) multiple supplement (MS), during the dry season of 1999 in Acre, Brazilian Amazon. The animals were maintained in pastures of Brachiaria decumbens, and liveweight gains, forage availability and chemical composition were assessed during the experimental period. Liveweight gains of 0.58, 0.65 and 0.60 kg/day, respectively, for MM, MMU and MS, were not different (P> 0.05). The use of MMU provided additional net economic return of 10.8% in comparison with the …

Roots And Potatoes As Supplements To An Alfalfa – Grass Diet To Dairy Cows, T. Eriksson, P. Ciszuk, E. Burstedt

IGC Proceedings (1977-2023)

Fourteen multiparous dairy cows were used in 3 x 3 change-over experiment designed to study effects of replacing barley by roots and potatoes in diets based on leguminous forage on feed intake, milk production and utilisation of feed nitrogen. The cows received a mixture of alfalfa (Medicago L.) and grass silage ad libitum and an isoenergetic quantity of a supplement based on either barley and potatoes (Ba/P), fodder beets (Beta vulgaris L.) and potatoes (Be/P) or barley (Ba). The grass in grass silage was dominated by timothy (Phleum pratense L.). Total DM intake as well as yield …

Agronomic Evaluation Of Forage Grasses Under Mature Rubber Plantation, N. L. Costa, Valdinei T. Paulino, A. C. M. Igreja, C. R. Townsend, J. A. Magalhães, R. G. De A Pereira, T. S. Paulino

IGC Proceedings (1977-2023)

The agronomic performance of seven forage grasses, under a mature rubber plantation, was assessed in a cutting trial carried out at Porto Velho, Rondônia. During the rainy season, the higher dry matter yields were obtained with B. brizantha, P. atratum BRA-9610 and B. humidicola. During the dry season, the grasses more productive were B. brizantha and P. atratum BRA-9610. In both seasons, magnesium and potassium contents did not vary among grasses. During the rainy season, the higher nitrogen and phosphorus contents were obtained with P. regnelli BRA-0159 and, P. guenoarum BRA-3824 and B. humidicola, respectively, while P. atratum …

Constraints And Opportunities For Development Of Grasslands In Urban Areas Of The Niayes Zone In Senegal, S. T. Fall, I. Cissé

IGC Proceedings (1977-2023)

A survey was carried out in urban areas of the Niayes zone in Senegal to explore grasslands development in agropastoral systems around towns. Rapid rural appraisal and farms multiple visits were done to describe the forage cropping system, identify constraints and opportunities of developing grasslands in urban areas. In Niayes urban areas, forage production system is being developed based on the cropping of several grass and tree species. Only a minority of farmers is involved in that activity. Level of grass production is rather low. Weak development of grasslands is observed. Main reasons are: farmer poor willingness to crop for …

Nutritive Quality Of Tropical Grasses Sown Under A Pine Plantation, D. Gutmanis, Antonio João Lourenço, V. B. G. Alcantara, Maria T. Colozza

IGC Proceedings (1977-2023)

A trial was conducted in Brazil to evaluate the nutritive quality of 6 tropical grasses: tanzania (Panicum maximum cv. Tanzania), green-panic (P. maximum var. Trichoglume), aruana (P. maximum cv. Aruana), brizanta (Brachiaria brizantha), humidicola (B. humidicola) and tifton-85 (Cynodon dactylon cv. Tifton 85), planted under two different densities of pines (Pinus elliottis): 200 and 400 stems/ha, as well as in full sunlight. The results showed a significant increase in protein content and a significant decrease in NDF and cellulose contents as tree density increased. Comparing the values obtained under shading conditions …

Production And Mineral Composition Of Tropical Grasses Sown Under A Pine Plantation, D. Gutmanis, V. B. G. Alcantara, Maria T. Colozza, Antonio João Lourenço

IGC Proceedings (1977-2023)

A trial was conducted in Brazil to evaluate the performance of 6 tropical grasses: tanzania (Panicum maximum cv. Tanzania), green-panic (P. maximum var. Trichoglume), aruana (P. maximum cv. Aruana), brizanta (Brachiaria brizantha), humidicola (B. humidicola) and tifton-85 (Cynodon dactylon cv. Tifton 85), planted under two different densities of pines (Pinus elliottis): 200 and 400 stems/ha, as well as in the full sunlight. The results showed that the dry matter yield decreased as shading increased. The grasses tanzania and brizanta were the most tolerant to shading. Although N concentration increased with …

Dry Matter Production And Nutritive Value Of Alfalfa (Medicago Sativa L.) And Orchardgrass (Dactylis Glomerata L.) Under Different Light Regimes, A. C. Varella, P. L. Peri, R. J. Lucas, D. J. Moot, D. L. Mcneil

IGC Proceedings (1977-2023)

Spring and summer alfalfa dry matter and nitrogen production rates were greater than orchardgrass in open pasture and under three shade levels. Ten years old Pinus radiata allowed 60% light transmissivity. The addition of artificial shade resulted in 45% and 25% transmissivity. Orchardgrass pastures were nitrogen deficient and water stressed. In contrast, alfalfa was not stressed and it was more light responsive than orchardgrass. Differences in nutritive value were relatively small between species and between light regimes. Sheep liveweight gain on alfalfa was greater than on orchardgrass. Orchardgrass demonstrated shade tolerance, but alfalfa was more productive even at 25% transmissivity. …

Optimizing Yield And Quality Of Orchardgrass Pasture In Temperate Silvipastoral Systems, P. L. Peri, R. J. Lucas, D. J. Moot, A. C. Varella, D. L. Mcneil

IGC Proceedings (1977-2023)

The objective of this study was to provide grazing management recommendations for a silvipastoral system by determining the effects of shade and nitrogen (N) on the pattern of dry matter accumulation and nutritive value of orchardgrass (Dactylis glomerata) in a subhumid temperate environment. Orchardgrass in open pasture (100% transmittance) and under 10 year old Pinus radiata tree shade (60% transmittance) at 200 stems/ha was used as the main plot and nitrogen (0 and 300 kg N/ha as synthetic urine) was the subplot factor. Dry matter (DM) production of orchardgrass in the first 60-day spring rotation was similar in …

Herbage Characteristics As Affected By The Canopies Of Dominant Trees In A Savanna Of Southern Mozambique, James P. Muir, A. Alage, Inacio C. Maposse

IGC Proceedings (1977-2023)

This study surveyed the species composition, forage yield and forage nitrogen concentration of the herbaceous layer 50, 100 and 200% of the canopy radius from the trunks of many-stemmed false thorn, knob thorn, scented thorn and marula trees in a southern Mozambican savanna. Tree species did not affect herbaceous DM yield or species diversity. Neither direction nor distance from the tree trunk affected DM or specific diversity. Crude protein percentages were 15% higher for legumes and 9% higher for grasses at 50% canopy than at 200% canopy. Grasses under the marulas, the only non-legume tree in the study, had 18% …

A Simulation Model To Evaluate Supplementation Of Tropical Forage Diets For Dairy Cows, A. G. Assis, O. F. Campos, J. Dijkstra, J. France

IGC Proceedings (1977-2023)

A dynamic model of digestion kinetics has been built to evaluate dairy cattle diets based on tropical feeds and to estimate the potential of tropical forages for milk production associated with available supplements. Results of simulation were very consistent showing that grazed elephant grass alone can supply nutrients for cow maintenance and yield of 7.10 kg milk/day. Nevertheless, to produce 25 kg/day on grazed elephant grass, a dairy cow would need to be supplemented with 5.85 kg/day of a mixture of cottonseed meal (50%) plus ground maize (50%), while on maize silage it would be necessary 4.15 kg of the …

Performance Of Beef Heifers Of Various Genetic Groups, Supplemented Or Not, In Coastcross Pastures, Armando De A. Rodrigues, Geraldo M. Da Cruz, Rogério T. Barbosa, Maurício M. De Alencar, Luciano De A. Corrêa, Gilson P. De Oliveira

IGC Proceedings (1977-2023)

The objective of this study was to determine whether the performance of beef heifers of different genetic groups was affected by breed x nutritional environment interactions. Sixty four weaned heifers, 16 per genetic group: ½ Angus + ½ Nellore (AN), ½ Canchim + ½ Nellore (CN), ½ Simmental + ½ Nellore (SN) and pure Nellore (NE), were used with or without 3.0 kg of concentrate.animal^-1 .day^-1 in a fertilized coastcross pasture under rotational grazing system. There were effects of genetic group and supplementation (P< 0.05) on the weight and age at first estrus, but there was no interaction between them. In a rotational grazing system with 4000 kg of available dry matter per hectare with 13% of crude protein, the crossbred AN, supplemented or not, was more precocious (111 days) than Nellore heifers, showing the first estrus at 356 days of age and 324 kg of live weight.

Urine Calcium But Not Plasma Calcium Or Urine Hydroxyproline Is Increased By A Systemic Acidosis In The Dairy Cow, J. R. Roche, Dawn E. Dalley, F. O’Mara, E. S. Kolver

IGC Proceedings (1977-2023)

Eight non-lactating, pregnant Holstein-Friesian cows were allocated to two treatments and individually offered diets differing in dietary cation-anion difference. Decreasing the dietary cation-anion difference reduced the urine pH within hours of anionic salt supplementation. Plasma calcium concentration was unaffected by dietary cation-anion difference but urine calcium concentration was significantly increased within 10 days of including anionic salts in the diet. Faecal calcium concentration was significantly reduced, indicating increased calcium absorption. Dietary calcium concentration or dietary cationanion difference did not significantly affect urinary hydroxyproline.

Dry Matter Intake Of Periparturient Cows On A Fresh Pasture/Pasture-Hay Diet, J. R. Roche, Dawn E. Dalley, F. O’Mara, E. S. Kolver

IGC Proceedings (1977-2023)

Reports of the depression in dry matter intake in the periparturient period have been inconsistent and little dry matter intake data is available for an all-forage diet prior to calving. Data to examine intake depression prior to calving was obtained from two experiments. In experiment 1, sixteen non-lactating, periparturient cows ate 1.3% of pre-calving body weight of grass-hay and freshly cut grass for two weeks pre-calving (restricted). Experiment 2 comprised thiry-six cows that ate 1.6% of pre-calving body weight of grass-hay and freshly cut grass for the final two weeks of pregnancy (ad libitum). Individual dry matter intakes …

Effectt Of Supplements On Forage Degradability Of Brachiaria Brizantha Cv. Marandu Grazed By Steers, J. R. A. Pereira, Ricardo A. Reis, L. R. De A. Rodrigues, D. Freitas

IGC Proceedings (1977-2023)

The supplementation effect was evaluated using crossbred rumen-fistulated steers maintained in a Brachiaria brizantha cv. marandu pasture. The animals received energy supplementation (corn-COR); low degradability protein supplementation (corn gluten-GLU); and high degradability protein supplementation (soybean-SBM) at two levels N1 (± 0.5 kg/an/day) and N2 (± 1.5 kg/an/day); or no supplementation (Control). The factorial (3x2), plus the control, was studied according to complete randomized block design with three replications. The supplementation did not affect (P> 0.05) the potential degradability (POD), the effective degradability (EFD), and the degradation rates (DR) of the forage (extrusa). The GLU supplementation provided higher (P< 0.05) EFD of the NIDN fraction than the SBM, and both did not differ (P> 0.05) of …

Use Of Supplements For Increasing Performances Of Suckling Martinik Ewes When Fed Tropical Forages, E. O. Jimenez, G. Alexandre, H. Archimède, P. Despois, M. Jean-Bart, C. Jacquet

IGC Proceedings (1977-2023)

Experiments have been carried out to determine the effects of supplementation upon milk production and growing performances with hair sheep Martinik ewes weighing 48 ± 4 kg liveweight (LW). In a first trial, 6 single bearing ewes (group GS) were offered individually a daily 0.5 kg of commercial pellets. They were compared to the control group (GN; 6 ewes) with no supplement during 10 weeks of lactation. Basal diet was chopped Digitaria decumbens grass ad libitum. In a second trial, level of supplementation was adapted to the ewes’ litter size: 6 twins bearing ewes (TW) and 6 single (SI). …

Mineral Concentration Of Herbage From Three Paulownia Species Used For Goat Browse, J-M. Luginbuhl, J. P. Mueller, B. A. Bergmann

IGC Proceedings (1977-2023)

Goats naturally prefer a high proportion of browse in their diets. Therefore, research was initiated to investigate various silvo-pastoral production systems. In May 1997, six Paulownia treatments were planted in a randomized complete block experiment with six replications. Trees were between 6 and 12 cm in height at planting. Treatments included: P. fortunei seedlings, P. tomentosa seedlings, P. elongata seedlings, and three P. elongata clones. Each 4-m wide plot contained a single row of 12 trees with an intra-row spacing of 1 m. Leaf samples were analyzed from all six replications to determine mineral concentration in October 1997, and June …