Digital Commons Network^™

Screening And Identification Of Antioxidant Peptides From Sea Cucumber Gonad Proteins And Their Activation Of Superoxide Dismutase, Zhiqin Zhang, Jingxuan Wang, Yongke Deng, Yugui Wang, Peipei Dou, Hongbing Fan, Xiangquan Zeng, Xinguang Fan, Lili Zhang, Haimei Liu, Qin Zhao

Animal and Food Sciences Faculty Publications

The gonad is one of the major byproducts of sea cucumber. Four novel antioxidant peptides (NPWGQ, PGHPF, VPYPR and ATGPQGPAGQRGPAGPTGPTGPAG) were isolated and identified from sea cucumber gonad proteins through enzymatic hydrolysis and antioxidant activity-guided fractionation, bioinformatics approaches and in silico screening. These peptides demonstrated great free radical (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS))-scavenging activity and notable superoxide dismutase (SOD)-activation capacity. Molecular docking and molecular dynamics simulation data suggested that these peptides could form strong binding with SOD through hydrogen bonding, electrostatic interactions, and hydrophobic interactions. Among these peptides, NPWGQ displayed the most potent antioxidant and SOD-activating effects. Through searching …

The Performance, Energy And Nutrient Utilization, And Bone Mineralization Of Broiler Chickens Fed Corn-Soybean Meal-Based Diets With Reduced Metabolizable Energy, Calcium, And Available Phosphorus Supplemented With Exogenous Enzymes, Megan M. Bauer, Tuoying Ao, Jacqueline P. Jacob, Michael J. Ford, Anthony J. Pescatore, Ronan F. Power, Sunday A. Adedokun

Animal and Food Sciences Faculty Publications

This study evaluated the performance and energy and nutrient utilization of broiler chickens fed corn-soybean meal-based diets supplemented with a naturally fermented enzyme complex containing xylanase and phytase. This was evaluated using 300 one-day-old male Cobb broiler chicks in a randomized complete block design with five dietary treatments replicated 10 times with 6 chicks per replicate. The treatments consisted of a positive control (PC) group containing commercially recommended energy and nutrient levels, a negative control (NC) group with reduced metabolizable energy, calcium, and available phosphorus, and three levels of exogenous enzyme supplementation to the NC diet at 150, 200, 250 …

Leucaena - A Permanent Dry Season Forage In Australia, J H. Wildin

IGC Proceedings (1977-2023)

Leucaena (Leucaena Jeucocephala) can play an important role in alleviating the general low pasture quality during the dry season. Extensive commercial use of leucaena for cattle grazing commenced in central Queensland in 1980 when the grazing management of this tree legume was modified to suit Australian beef producers. Plantings increased from 30 ha in 1979 to 3000 ha in central Queensland by February 1985. This rapid expansion can be attributed to beef producers being more aware ofleucaena management and benefits based on several commercial successes. Mimosine toxicity has not been a problem when cattle have access to tree …

Effect Of Some Agronomic Practices On Productivity And Nutritive Analysis Of Aquatic Grass Echinochloa Stagnina, K M. Ghobrial, M A. Gabra, M A. Noreldin, M A. Salem

IGC Proceedings (1977-2023)

Results indicated that the higher green, dry and protein yields increased with increasing plant density up to 105,000 plant per feddan that were fertilized with 45 kg N/fed. The average of the obtained green, dry and protein yields were 14.16, 4.24 and 0.30 ton/fed. respectively. The nutritive analysis on DM basis was 7.13 % crude protein (CP), 2.12% ether extract (EE), 31.56% crude fidre (CF), 47.97% nitrogen free extract (NFE) and 11.22% ash. The calculated starch value (SV) as a feed unit was 1.34 ton/feddan.

The Role Of Temperature Pastures Species In Beef Farming In The Humaid Sub-Tropics Of South America, Y E. Kawabe, E Hayashi

IGC Proceedings (1977-2023)

Attempts were made to identify and solve the problems which a beef farming operation in the humid sub-tropics of South America was facing. A total of 370ha of pasture at C.A.O.S.A. was established using temperate species, Lolium perenne, L. multiflorum, Trifolium repens, T. subterraneum and Medicago sativa, both to establish new and also to improve existing tropical pasture in order to overcome: 1. Cool season feed shortage., 2. Low nutritional value of tropical grasses. A rotational grazing system was introduced and the yield and feed value analysis showed a substantial increase in cool season feed while the …

The Effect Of Row Width And Intrarow Spacing On The Productivity And Nutritional Quality Of Silage Corn, Y Kishida, S Uchida

IGC Proceedings (1977-2023)

This study was conducted to determine the effect of planting density on silage corn production and silage quality. Dent corn cv. Pioneer 3424 was planted at different or the same densities by changing row width or intrarow spacing or the both. The dry matter yield and silage quality were determined at different stages of ripening and the results are summarized as follows: When intrarow spacing was changed (under different intrarow spacings), the rate of increase of dry matter per plant was high at the low and intermediate planting densities and the dry matter yield per 10 are was high at …

Effects Of Climatic Zone, Topography, Land Utilization And Soil Condition On The Mineral Content Of Natural Grasses In Bali, K Lana, I M. Nitis, S Uchida

IGC Proceedings (1977-2023)

To evaluate nutritional quality of the natural grasses fed to livestock, grasses grown in different climatic zones (zones B, C, D, E and F), different topographies ( coast, hills and higher altitude), various forms of land utilization (rice field, dry land and plantation) and different soil conditions, allocated in 15 districts of 8 regencies in Bali were analyzed for their macro and micro element contents. Although P, K, Mn and Mo contents were higher and Mg, Na, Fe and Zn contents were lower in wet season than in dry season, Ca and Cu contents were constant throughout the seasons. The …

Potential Role For Stylo In Traditional Crop And Livestock Production Systems In Nigerian Subhumid Zone, M A. Mohamed Saleem, R Von Kaufman, R M. Otsynia, H Suleiman

IGC Proceedings (1977-2023)

The typical soils (Alfisols) of the subhumid zone of Nigeria deteriorate under continuous cropping and grazing. Due to increasing demand for land, soil recovery through natural fallowing is too time consuming. Because other reclamation techniques are expensive forage legumes that rapidly confer most fallow benefits and improve livestock nutrition have an important role in traditional crop-livestock production systems. In its experiments ILCA subhumid zone team used Stylosanthes spp. (stylo) in the following way: - (i) undersown or sown on alternate ridges with sorghum, (ii) sorghum transplanted into it, and (iii) the succeeded by maize after varying years of its growth. …

Effects Of The Association Corn-Soybean On Dry Matter Production And Nutritional Silage Value, R Garcia, A R. Evangelista, J D. Galvao

IGC Proceedings (1977-2023)

Experiments were conducted to study the influence of corn-soybeans plant population in five cropping systems (four com-soybean associations and single corn) on the dry matter production and nutritional silage value. Variation in corn plant populations did not influenced green matter production, however, dry matter production increased as population increase. The cropping systems influenced both green and dry matter production. Plant population influenced on the nutritional silage value. The protein content and dry matter digestibility was higher for 30 thousand plants of corn per hectare. Two rows of corn to four of soybean resulted in superior protein content. The association com-soybean …

Managerial Characteristics Of The Grassland Type Dairy Farming In Hokkaido, Japan, Y Miyazawa

IGC Proceedings (1977-2023)

Hokkaido is the northernmost island of Japan with an area of about 78,500 km2 and lies between 41 ° and 46°N. Dairy farming in Hokkaido, being endowed with vast land resources, has overcome severe climatic conditions and expanded its scale of operations through such measures as developing and improving grassland. Its main type of dairy farming can be said to be of the-grassland-type and the feeding resources of which depends mainly on pastures. The dairy farming in Hokkaido, therefore, is represented by this grassland-type that is one of the alternative types to be developed under comparatively unfavorable land conditions, such …

Weight Gains And Nutrition Of Cattle Grazing On Grasslands Of Central Interior British Columbia, Canada, D A. Quinton, L Stroesser, H Douwes

IGC Proceedings (1977-2023)

Average daily gains and nutrient deficiencies of cattle grazing three grassland zones in British Columbia are shown. Cattle production was greatest on the rough fescue (Festuca scabrella) -bluebunch wheatgrass (Agropyron spicatum) association and least on the big sagebrush (Artemisia tridentata) -bluebunch wheatgrass association. Nitrogen (N), phosphorus (P) and magnesium (Mg) concentrations in cattle diets generally paralleled cattle gains. Increases in these nutrients in forages correlate to increased cattle gains. Depressed weight gains, poor growth of calves, late estrous cycling of cows, and white muscle disease of calves are all livestock symptoms associated with nutrient deficiencies of cattle …

The Effect Of Fertilization With N-P-K In Yield Of Natural Pasture Grazed By Sheep On Southern Italy, L Postiglione, F Basso, T Di Ella, F Carone

IGC Proceedings (1977-2023)

In order to raise the very low pasture yield in the dry regions of Southern Italy, a research was undertaken on the efficiency of mineral fertilization, owing to the variability of climatic conditions. Two levels of N (0-50 Kg ha-1), P (0-100 Kg ha-1) and K (0-100 Kg ha-1) arranged in factorial combinations were compared with a pasture grazed by sheep in a hilly area of Basilicata region (550 m s.l.} since 1982. During the spring of the first two years rainfalls amounted respectively to 79 mm and 146 mm while maximum temperature reached 30°C and 22°C in May, therefore …

Influence Of Stocking Rate On The Plant-Animal Interface In Rotational Grazed Mixed Swards, J W. Stuth, J R. Brown, P D. Olson, M R. Araujo

IGC Proceedings (1977-2023)

The impacts of brief periods of intense grazing in multipasture-single herd grazing systems on critical plant and animal parameters in natural mixed swards are not well understood. It was our objective to determine the interrelationship and nutritional consequence of tiller defoliation intensity, selection ratio and diet composition of two codominant species with contrasting phenology and growth habit as stocking rate was increased. A 16-pasture, 1-herd short-duration grazing regime was simulated at four stocking rates on a little bluestem (Schizachyr­ium scoparium)-brownseed paspalum (Paspalum plicatulum) savannah. Variables measured included morphology and defoliation intensity of marked tillers, diet composition …

The Effect Of Fertilization With N-P-K On Utilization And Nutritive Value Of Natural Pasture Grazed By Sheep In Southern Italy, B Ferrara, V Piccolo, F Basso, A Nizza

IGC Proceedings (1977-2023)

Results of four grazing periods carried-out in 1983 and 1984 in a hilly area of Basilicata region (550 m. above s.I.) on a natural pasture utilized by sheep are reported here. Two levels of N(0-50 kg ha-1), P(0-100 kg ha-1) and K(0-100 kg ha-1) arranged in eight factorial combinations were compared. The utilization coefficients of dry matter (DM), crude protein and milk-FU (MFU) were affected by the climatic conditions and grazing period, whereas fertilization effect was low. The influence of treatments was not very emphasized during the first experimental phase also on the nuttritive value of total and utilized forage.

Vegetational Changes In The Sasa Nipponica Grassland Used By Winter Grazing For Successive Years, Y Ogawa, T Mitamura, K Okamoto

IGC Proceedings (1977-2023)

The influence of winter grazing by beef cattle on yearly yield and flora changes in Sasa (Sasa nipponica) grassland was examined from 1974 to 1982. This Sasa sward was established semi-naturally on cutover land following deciduous broadleaved forest at an altitude of 1,100 m on Mt. Asama in Japan. Sasa kept its foliage green and nutritive all year round, so that in this experimental grassland a large quantity of its foliage supplied sufficient nutrients for the maintenance of beef cows during winter grazing periods. Foliage weight was 2.6 tons DW/ha before winter grazing use and this value was maintained for …

Grassland Resources And Future Development Of Grassland Farming In Temperate China, Zhu Tingcheng, Li Jiandong, Zu Yuangang

IGC Proceedings (1977-2023)

In the first part of the paper the grassland resources in the semiarid area of China are descrived. Grasslands cover 2.86 million km2 of the total territory and 100 million livestock are being raised there. Steppes and meadows are classified into several types according to the ecological types of the dominant species and environments, etc. Area, geographical distribution, community structure, species composition, dynamics, nutritive value of herbages, primary production and utilization of each grassland type are outlined. In the second part, the necessity to promote grassland farming and the possibility of its development are discussed based upon the land, population, …

Do Honey Phytochemicals Modulate Forager Aggression And The Gut Microbiome In The Honey Bee (Apis Mellifera L.)?, Wade A. Pike, Jaesylin Stephens, Mariah Donohue, Katsuri Rajandran, Erin D. Tranore, Abdallah Sher, Emily Croteau, Clare C. Rittschof

Biology Faculty Publications

Plant phytochemicals found in nectar impact bee learning and memory and plant pollination success. Especially for generalist pollinators, dietary changes that alter phytochemical consumption could be common sources of behavioral variation. For honey bee (Apis mellifera L.) foragers, a major potential change in phytochemical consumption occurs when individuals switch from collecting nectar from flowers to collecting honey from neighboring colonies, a phenomenon known as honey robbing. In this study we investigated whether phytochemicals dominant in honey compared to nectar act as a short-term trigger of robbing behaviors in honey bee, which include increased aggression. We fed forager honey bees sucrose …

An Animal Interest Approach Method As A Gateway To A One Health Educated Pipeline, Meghan C. Wulster-Radcliffe, Sarah A. Reed, James N. Macleod, Rebekah B. Epps, J. Scott Radcliffe, Robert J. Coleman, Steven A. Zinn

Animal and Food Sciences Faculty Publications

No abstract provided.

Outcomes Of Liver Transplantation In Patients With Severe Obesity, Alejandro Cracco, Mackenzie Donoho, Siddharth Desai, Meera Gupta, Alexandre Ancheta, Malay B. Shah, Xiaonan Mei, Francesc Marti, Roberto Gedaly

Surgery Faculty Publications

Background. – This study aims to evaluate the impact of obesity on post–liver transplant outcomes stratified by low- and high-performance status. Methods. – Patients undergoing liver transplantation between 2006 and 2023 were identified in the United Network for Organ Sharing data set. Patients with severe obesity body mass index ([BMI] ≥40 kg/m²) and super obesity (BMI >50 kg/m²) were compared with those with lower BMI on perioperative and long-term outcomes stratified by preoperative performance status. Results. – Severe obesity has a significant negative impact on perioperative and long-term outcomes on the low-performance group (LPG). This effect …

Abnormal Invasive Hemodynamics In Heart Transplant Recipients, Navin Rajagopalan, Donna R. Dennis, Julia Akhtarekhavari, Kenneth Campbell

Physiology Faculty Publications

BACKGROUND Few studies have quantified invasive hemodynamic parameters in post heart transplant recipients. AIM To report the incidence of abnormal hemodynamics in heart transplant recipients at 1-year and 3-year post-transplant and determine if there was any correlation with recipient and donor characteristics. METHODS Data from 279 consecutive heart transplant recipients from 2007 through 2020 were analyzed. Clinical variables regarding both recipients and donors as well as hemodynamic variables obtained via right heart catheterization during 1-year and 3-year annual testing were recorded. Simple and multiple linear regression tests were used to determine how recipient and donor variables influenced hemodynamic parameters at …

Evidence-Based Best Practices For Diabetes Education And Self-Care In Appalachia: A Literature Review, Angela J. Occidental, Inga M. Zadvinskis, Jacqueline Hoying

Journal of Appalachian Health

Introduction: The Appalachian Region has a higher prevalence of type 2 diabetes mellitus (T2DM) and associated adverse health outcomes. Although numerous papers report best practices for diabetes care and education, a clinician-friendly, synthesized summary of best practices tailored to Appalachian cultural preferences is lacking.

Purpose: This paper uses the Melnyk & Fineout-Overholt evidence-based framework to identify the best practices for T2DM care and education for Appalachian residents.

Methods: A comprehensive literature search using the databases CINAHL, Academic Search Complete, and PubMed was guided by a PICOT question. Quality appraisals were completed for fifty-two articles; thirty papers were selected to synthesize …

Radon Environmental Health Literacy Among Health Council Participants In Northeast Tennessee, Kawther Al Ksir, Stacy Stanifer, Hadii M. Mamudu, Megan Quinn, Deborah L. Slawson

Journal of Appalachian Health

Introduction: Radon, a radioactive gas, poses significant health risks, including lung cancer, and is prevalent in indoor environments. Understanding radon testing behavior and Environmental Health Literacy (EHL) is crucial for individuals to take appropriate preventive measures.

Purpose: This study assessed radon-related EHL among Health Council participants in seven Northeast Tennessee (NETN) counties and examined associations between sociodemographic factors and EHL levels. Understanding radon knowledge and behaviors within this influential group is critical, as they often serve as trusted public health advocates.

Methods: A cross-sectional survey was conducted from September – November 2023 among Health Council participants across seven counties in …

Prevalence Of Screening Patients For Alcohol Use In West Virginia: A Cross-Sectional Observational Study, Bayan Abuhalimeh, Christopher Waters, R. Constance Wiener

Journal of Appalachian Health

Introduction: Consequences of alcohol consumption are well-established and compounded by physiological changes of aging (increased sensitivity; medication interactions; etc.). Alcohol screening for all ages is recommended, especially as most Americans believe moderate drinking is acceptable. Screening is important in high alcohol use regions, such as rural areas where isolation is more likely, especially for older adults. The Appalachian Region has a significant rural population of older adults. West Virginia (WV) is representative of Appalachia. It is a rural state, has many older adults, and all counties are Appalachian.

Purpose: The purpose of this cross-sectional study is to determine the likelihood …

Addressing The Root Causes Of Population Health In Central Appalachian Virginia, David L. Driscoll, Kol Gold

Journal of Appalachian Health

Introduction: The Southwest Virginia Health Authority (SWVHA) recently commissioned a community health needs assessment (CHNA) to reduce the high rate of preventable health problems in Virginia’s (VA) Appalachian Region. The CHNA took place over a two-year period from 2023 – 2025.

Purpose: This iterative, mixed-method CHNA informed the development of a new iteration of the regional Blueprint for Health Improvement and Health-Enabled Prosperity identifying and prioritizing health issues and develop strategic planning to address them in VA’s three westernmost regional health districts.

Methods: The CHNA followed a sequential mixed methods design to assess the regional health status, the contextual factors …

Lung Cancer Prevention Among Appalachian Kentucky Women, Jessica R. Thompson, Nancy E. Schoenberg, Pamela C. Hull

Anthropology Faculty Publications

Purpose: Appalachian Kentucky (KY) residents experience the highest lung cancer rates in the US with declines lagging among women; we sought to uncover barriers and facilitators to lung cancer prevention for Appalachian KY women and to identify community-specific interventions. Approach: We utilized concept mapping, a participatory mixed method, to generate consensus on perceived barriers and facilitators. Setting/Participants: We recruited 71 adult women from Appalachian KY counties. Method: After collecting online concept mapping data, we used multidimensional scaling to generate a point map of perceived similarities and hierarchical cluster analysis to create a thematic cluster map. We compared average cluster ratings …

Multidimensional Biocircuitry Of Exercise Adaptation, Jeremy S. Mcadam, Zachary A. Graham, S. Craig Tuggle, Kaleen M. Lavin, Marcas M. Bamman, Jeremy S. Mcadam, Zachary A. Graham, Brandon Peoples, Regina S. Seay, Kaleen M. Lavin, Amber B. Gargus, Sufen Yang, Samia M. O’Bryan, Devin J. Drummer, Christian J. Kelley, Margaret B. Bell, Inmaculada Aban, Gary R. Cutter, Marcas M. Bamman, Michael P. Craig, Jin Zhang, Akshay Hira, Timothy J. Broderick, Madhavi P. Kadakia, Zachary A. Graham, Kalyani Peri, Margaret B. Bell, Inmaculada Aban

Physiology Faculty Publications

In a randomized, dose-response trial, we used molecular and phenomic profiling to compare responses with traditional moder ate-intensity endurance and resistance training (TRAD) versus high-intensity tactical training (HITT) that encompassed explosive whole-body interval training and high-intensity resistance training. Ninety-four participants (18–27 yr) completed 12 wk of TRAD or HITT followed by 4 wk of detraining. Although similar performance and body composition improvements were observed in response to HITT and TRAD, some dose-dependent differences were observed for: 1) ex vivo muscle tissue changes in myofiber size, capillarization, satellite cell frequency, and mitochondrial function and 2) differential gene expression (DGE) of muscle …

Satellite Cells Choreograph An Immune Cell-Fibrogenic Cell Circuit During Mechanical Loading In Geriatric Skeletal Muscle, Nicholas T. Thomas, Camille R. Brightwell, Allison M. Owen, Alexander R. Keeble, Sabin Khadgi, Yuan Wen, Christopher S. Fry, Kevin A. Murach

Physiology Faculty Publications

Muscle stem cells, or satellite cells (SCs), decline in number throughout the lifespan and may become senescent in very old age. Whether and how remaining SCs contribute to muscle adaptation in the oldest-old is unclear. Using acute mechanical overload in geriatric SC replete and depleted mice (28-month-old) combined with single-cell RNA-sequencing, we show: (i) subsets of geriatric SCs display signs of senescence as well as normal fate progression during overload, (ii) SCs express markers that may contribute to the regulation of innervation, (iii) the presence of SCs during overload enhances global intercellular communication and increases mRNA levels of the cell …

Identification Of Metabolic Pathways And Hub Genes Associated With Ultrasound Subcutaneous Fat And Muscle Depth Of The Longissimus Muscle In Cull Beef Cows Using Gene Co-Expression Analysis, Harshraj Shinde, Kyle R. Mcleod, Jeffrey W. Lehmkuhler

Animal and Food Sciences Faculty Publications

Beef production is an important component of the world’s food supply, with production being near 59 million tons in 2023 (USDA, 2023). Enhancing our understanding of the factors influencing metabolism will lead to improvements in production efficiency. Using RNA-seq and WGCNA of longissimus muscle samples, gene expression and metabolic pathway analyses were performed to examine relationships with ultrasound and body mass variables. In this study, body weight (BW), ultrasound back fat (BF), ultrasound muscle depth (MD), and body condition score (BCS) were traits recorded for 18 cull beef cows. As expected, all production-related traits monitored (WT, BF, MD, and BCS) …

“Literally Made Me Cry”: The Impact Of Course Material Costs On University Of Kentucky Students, Stephen Krueger

Library Reports and White Papers

The Student Course Materials Cost Survey was administered September 16 – October 6, 2024. It was open to current students at the University of Kentucky (UK). The goals were to help UK Libraries better understand how the cost of course materials affects students at UK and to provide a snapshot of student spending across the university. The questions were based on similar research done at other institutions in Virginia (Virginia Course Materials Survey, 2021) and Florida (2022 Student Textbook and Instructional Materials Survey, 2022). Other work based on those surveys includes the 2023 Illinois Course Materials Survey (CARLI, 2023) and …

Making Sense Of Myc In Skeletal Muscle, Ronald G. Jones, Sebastian Edman, Nathan Serrano, Yuan Wen, John J. Mccarthy, Christopher S. Fry, Ferdinand Von Walden, Kevin A. Murach

Physiology Faculty Publications

No abstract provided.